Leica M80 B User Manual
Living up to Life
User Manual
Leica M60 B
Leica M80 B
Copyright
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method
(including electronic systems) is not allowed
without express written permission from Leica
Microsystems CMS GmbH.
The instructions contained in the following
documentation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible. Still, we are
always grateful for comments and suggestions
regarding potential mistakes within this documentation.
The information included in this manual may
be changed without prior notice.
Revision 1.0, published March 14, 2013 by:
Leica Microsystems Wetzlar GmbH
Ernst Leitz-Straße 17-37
D-35578 Wetzlar (Germany)
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS
Leica M series User Manual 2
Function of the Microscopes
The Leica M60 B and Leica M80 B microscopes,
for which this User Manual has been written,
are designed for routine examinations of cell
and tissue cultures, liquids and sediments. This
includes examining specimens taken from the
human body for the purpose of gaining information about physiological or pathological
conditions or inborn anomalies, or testing for
safety and compatibility for potential recipients, or for monitoring therapeutic measures.
IVD
The above-named microscopes comply with
the Council Directive 98/79/EC concerning in
vitro diagnostics.
The manufacturer assumes no liability for damage caused by, or any
risks arising from, using the microscope for
other purposes than those for which they are
intended or not using them within the specifications of Leica Microsystems CMS GmbH.
In such cases, the Declaration of Conformity
shall be invalid.
Leica M60 B nameplate
These (IVD) instruments are not
intended for use in the patient environ-
ment defined by DIN VDE 0100-710. Nor are
they designed to be combined with medical
instruments in accordance with EN 60601-1. If a
microscope is electrically connected to a medical
instrument in accordance with EN 60601-1, the
requirements listed in EN 60601-1-1 shall apply.
Not suitable for examining potentially infectious specimens. This type of instrument may
be operated by trained laboratory personnel
only.
Leica M80 B nameplate
Leica M series User Manual 3
General Notes
Use in clean rooms
The Leica M series can be used in clean rooms
without any problems.
Cleaning
★ Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
★ Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and products could be contaminated by
abraded particles.
★ In most cases, we can provide special solu-
tions on request. Some products can be
modified, and we can offer other accessories for use in clean rooms.
★ The cleaning of glass surfaces and objec-
tives in particular should be carried out
exclusively as outlined in the brochure
"Cleaning of Microscope Optics". The information can be downloaded at:
http://www.leica-microsystems.com/prod-
ucts/
. Select your product and go to the "Down-
load" page.
For additional information, refer to page 55.
Servicing
★ Repairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
★ Ensure that the Leica stereomicroscope
is operated, maintained and repaired by
authorized and trained personnel only.
Leica M series User Manual 4
Important Safety Notes
User Manual
This User Manual describes the special functions of the individual modules of the Leica M
stereomicroscopy series and contains important instructions for their operational safety,
maintenance, and accessories.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the User Manual
and the safety instructions from the supplier.
Before installing, operating or using the instruments, read the user manuals listed above. In
particular, please follow all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
We guarantee the quality of our products.
Our guarantee covers all faults in materials
and manufacture. It does not, however, cover
damage resulting from careless or improper
handling.
Leica M series User Manual 5
Symbols Used
Warning! Safety hazard!
This symbol indicates especially important information that is mandatory to
read and observe.
Failure to comply can cause the following:
★ Hazards to personnel
★ Functional disturbances or damaged
instruments
Warning of hazardous electrical voltage
This symbol indicates especially important information that is mandatory to
read and observe.
Failure to comply can cause the following:
★ Hazards to personnel
★ Functional disturbances or damaged
instruments
Danger due to hot surface.
This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional information or explanations that are intended
to provide clarity.
Explanatory notes
★ This symbol within the text stands for addi-
tional information and explanations.
Figures
(1) Numbers in parentheses within the
descriptions relate to the figures and the
items within those figures.
Disposal
Notes on how to dispose of the microscope, its components and expend-
ables.
China RoHS 50 year EFUP (Environment-Friendly Use Period)
IVD labeling
nstrument for in vitro diagnostics.
IVD
I
IVD manufacturing date,
for example 11 / 2011 for
MM/YYYY
November 2011.
Leica M series User Manual 6
Safety Instructions
Description
★ The individual modules fulfill the highest
requirements for observation and documentation of Leica stereomicroscopes of
the M series.
Intended use of instrument
★ Leica Microsystems microscopes are opti-
cal instruments for improving the visibility
of objects or specimens through magnification. Accessories such as optical accessories, stands, illumination, cameras etc.
supplement the equipment configuration.
Non-intended use
★ Using the instrument in any way contrary
to the specifications in the User Manual can
lead to bodily harm and damage to objects.
Never use microscopes for in vivo examinations or eye surgery if they are not expressly
intended for such use. Never install any
other plug or unscrew optical systems
and mechanical parts unless expressly
instructed to do so in the instructions.
The instruments and accessories described in
this User Manual have been tested for safety and
potential hazards. The responsible Leica affiliate
must be consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual!
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims as well as product liability and
the Declaration of Conformity.
Place of use
★ Only use the instruments in enclosed,
dust-free rooms and between +10 °C and
+40 °C. Protect the devices from oil, chemicals and extreme humidity. If using the
devices outdoors, protect them from dust
and moisture. Never use electrical devices
outdoors.
★ Electrical components must be placed at
least 10 cm away from the wall and away
from flammable substances.
★ Avoid large temperature fluctuations,
direct sunlight and vibrations. These conditions can distort micrographic images, for
example.
★ In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.
Leica M series User Manual 7
Safety Instructions (continued)
Responsibilities of person in charge of
instrument
★ These Safety Instructions must be available
at the workplace.
Ensure that:
★ The M series of stereomicroscopes and
accessories are operated, maintained and
repaired by authorized and trained personnel only.
★ All operators have read, understood and
observe this User Manual, and particularly
the safety regulations.
Repairs, service work
★ Repairs may only be carried out by Leica
Microsystems-trained service technicians.
★ Only original Leica Microsystems spare
parts may be used.
★ Before opening the instruments, switch off
the power and unplug the power cable.
★ Avoid contact with powered electrical
circuits, which can lead to injury.
Transport
★ Use the original packaging for shipping or
transporting the individual modules of the
Leica M stereomicroscopy series and the
accessory components.
★ In order to prevent damage from vibrations,
disassemble all moving parts that (according to the user manual) can be assembled
and disassembled by the customer and
pack them separately.
Integration in third-party products
★ When installing Leica products into third-
party products, the manufacturer of the
complete system or its dealer is responsible
for following all applicable safety instructions, laws and guidelines.
Disposal
★ Once the product has reached the end of its
service life, please contact Leica Service or
Sales about disposal.
★ Please observe and comply with the
national and federal laws and regulations
that are equivalent to EC directives such as
WEEE.
Like all electronic devices, the microscope, its accessory components and
consumables must never be disposed of with
general household waste.
Leica M series User Manual 8
Safety Instructions (continued)
Legal regulations
★ Observe the generally applicable statu-
tory and country-specific regulations for
accident prevention and environmental
protection.
EC Declaration of Conformity
★ Electrically operated accessories are
constructed based on the state of the art
of technology and are provided with an EC
Declaration of Conformity. See page 54.
Health risks
Workplaces with stereomicroscopes facilitate
and improve the viewing task, but they also
impose high demands on the eyes and holding
muscles of the user. Depending on the duration of uninterrupted work, asthenopia and
musculoskeletal problems may occur. For this
reason, appropriate measures for reduction of
the workload must be taken:
Optimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently). Thorough training of the personnel, giving consideration to ergonomic and
organizational aspects.
The ergonomic design and construction of the
Leica M stereomicroscopy series are intended to
reduce the exertion of the user to a minimum.
Direct contact with eyepieces can be a potential
transmission path for bacterial and viral infections of the eye.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.
Careful handling
★ Exercise particular care when setting up
the instruments. If it is specified that two or
more people are required for setup, compliance with this is mandatory.
★ Never spill any liquids on electrical instru-
ments. This could cause the stereomicroscope and other equipment to become
electrically live and damage people and
instruments.
★ Never clean instruments using corro-
sive cleaning agents or those containing
acetone. For detailed information about
care, refer to the User Manual for the instrument.
★ Check the power cables regularly. Defective
power cables can cause injuries.
★ Wait for bulbs to cool off before changing
them. Touching hot bulbs can cause burns.
Leica M series User Manual 9
Safety Instructions (continued)
Light sources: safety regulations
★ Light sources pose a potential irradia-
tion risk (glare, UV radiation, IR radiation).
Therefore, lamps have to be operated in
closed housings and in installed condition.
★ Never look directly into the beam path
(blinding hazard).
★ Do not select a white, strongly reflective
background for the specimen.
External power supply for the TL5000 Ergo
(Leica M80 B)
Permitted power supply:
SINPRO SPU130-110
Specifications:
Input: 100-240 V AC
47-63 Hz
3.2 A
Output: 33 V DC
3.93 A
max. 130 W
For indoor use only.
Use only the power supply specified
above. Other power supplies must not
be used. If the original power supply fails or
is damaged, it must be replaced. Repair is not
permitted. Original power supplies are available from your Leica branch office or Leica
dealer.
Leica M series User Manual 10
Contents
Copyright 2
Function of the Microscopes 3
General Notes 4
Important Safety Notes 5
Symbols Used 6
Safety Instructions 7
Leica M Series
Congratulations! 14
Maximum Compatibility 15
On We Go 16
Assembly
Base and Focusing Column 18
Microscope Carrier 19
Optics Carrier 20
Tube 21
Eyepieces 22
Objective 23
TL ST Transmitted Light Base (for Leica M60 B) and
TL5000 Ergo Transmitted Light Base (for Leica M80 B) 24
Quick Start Guide
The Reliable Route to Success 26
Overview of an M Series Microscope 27
The Correct Interpupillary Distance 28
Using the Eyepieces 29
Focusing 30
Adjusting the Resistance of the Focus Drive 31
Changing the Magnification (Zoom) 32
Click Stops and Magnification Levels 33
Eyepieces
Magnification Factor of the Eyepieces 35
Health Notes 36
Dioptric Correction 37
Dioptric Correction and Parfocality 38
Graticules 39
Objectives and
Optical Accessories
The Different Types of Objectives 41
Leica M series User Manual 11
Bases
Leica TL ST Transmitted Light Base: Controls (Leica M60 B) 43
Leica TL ST Transmitted Light Base: Operation 44
Leica TL ST Transmitted Light Base: Lamp Replacement 45
Leica TL5000 Ergo Transmitted Light Base: Controls (Leica M80 B) 46
Leica TL5000 Ergo Transmitted Light Base: Operation 47
Specifications for the Bases
Leica TL ST Transmitted Light Base 50
Leica TL5000 Ergo Transmitted Light Base 51
Appendix
Calculating the Total Magnification and Field of View Diameter 53
Care, Maintenance, Contact Persons 54
Leica M series User Manual 12
Leica M Series
Leica M series User Manual 13
Congratulations!
The New Leica M Series
Leica Microsystems introduces the Leica M60 B and M80 B, two new highquality routine stereomicroscopes of the CMO product line from Leica
Microsystems. The optical brilliance and wide range of accessories make
them the ideal stereomicroscopes for quality assurance and for in-vitro
diagnostics examinations.
The Leica M60 B and M80 B stereomicroscopes can be used for a wide
range of routine applications with the 6:1 and 8:1 zoom and engaged,
precise click-stops at predetermined magnifications. The large working
distance and brilliant imaging power show the finest details of your specimens without losing the field of view over large specimens.
Common to all three microscopes is the Leica range of accessories.
Whether the work requires a variety of illumination types, a wide selection of objectives, or the Leica swing-arm system – Leica Microsystems has
a solution for everything!
Do you already own stereomicroscopic equipment and are thinking of
switching to Leica? No problem! The Leica M60B and M80 B fit into the
same 76 mm diameter interface microscope carriers as with previous
models and are therefore compatible with many suppliers. They adapt
easily to existing components and add the high-quality imaging power of
Leica stereomicroscopes to existing inspection processes.
Leica M series User Manual 14
Maximum Compatibility
Leica engineers were careful to ensure that
the new Leica M series – like its predecessors
– remains compatible with existing series. This
means that objectives, bases, tubes and so on
can be reused.
Objectives
All objectives of this new generation have the
same reference focal length as the objectives of
the MZ generation. If you prefer, you can therefore continue to use the previous Leica objective series.
Tubes
The interface between the optics carrier and
the tube has remained the same, so existing tubes fit the new M series. The new tubes
are designed for eyepieces with field number
23, while the predecessor models were only
designed for field number 21, resulting in a
smaller object field.
Eyepieces
The new M series eyepieces have an audible
and tangible click to provide immediate feedback in case of accidental adjustment.
Leica M series User Manual 15
On We Go
If your new Leica microscope has already
been assembled and commissioned by your
Leica consultant, click here to skip through
the installation instructions and go directly
to the Quick Start Guide on page 25
.
If, on the other hand, you are assembling your
Leica microscope yourself, continue with the
"Assembly" chapter, which begins on page
17.
Leica M series User Manual 16
Assembly
Leica M series User Manual 17
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