Leica M60, M80 User Manual

Leica M50

Leica M60

Leica M80

User Manual

General Instructions

Safety concept

Before using your microscope for the first time, please read the "Safety concept" brochure included with your instrument. It contains additional information about handling and care.

Use in clean rooms

The Leica M series can be used in clean rooms without any problems.

Cleaning

Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning.

Never use chemicals to clean colored surfaces or accessories with rubberized parts. This may damage surfaces and abraded particles may contaminate preparations.

In most cases, we can provide special solutions on request. Some products can be modified, and we can offer other accessories for use in clean rooms.

Servicing

Repairs may only be carried out by Leica Microsystems-trained service technicians. Only original Leica Microsystems spare parts may be used.

Responsibilities of person in charge of instrument

Ensure that the Leica stereomicroscope is operated, maintained and repaired by authorized and trained personnel only.

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Important Safety Notes

User Manual

The individual modules of the Leica M stereomicroscopy series include an interactive CD-ROM with all relevant user manuals in 20 other languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.stereomicroscopy.com.

This operating manual describes the special functions of the individual modules of the Leica M stereomicroscopy series and contains important instructions for their operational safety, maintenance, and accessories.

The"Safetyconcept"bookletcontainsadditional safety information regarding the service work, requirements and the handling of the stereomicroscope, accessories and electrical accessories as well as general safety instructions­ .

You can combine individual system articles with articles from external suppliers (e.g. cold light sources, etc.). Please read the user manual and the safety requirements of the supplier.

Before installing, operating or using the instruments, read the user manuals listed above. In particular, please observe all safety regulations.

To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.

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Symbols Used

Warning of a danger

•This symbol indicates especially important information that must be read and

complied with.

Failure to comply can cause the following:Hazards to personnel

Functional disturbances or damaged ­instruments

Warning of hazardous electrical voltage

This symbol indicates especially important information that is mandatory to

read and observe.

Failure to comply can cause the following:Hazards to personnel

Functional disturbances or damaged instruments­

Danger due to hot surface.

This symbol warns against touching hot surfaces, e.g. those of light bulbs.

Important information

This symbol indicates additional information or explanations that are intended to

provide clarity.

Explanatory notes

This symbol within the text stands for additional information and explanations.

Figures

(1)Numbers in parentheses within the descriptions relate to the figures and the items within those figures.

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Safety Regulations

Description

The individual modules fulfill the highest requirements for observation and documentation of Leica stereomicroscopes of the M series.

Designated use

Refer to "Safety Concept" booklet

Non-intended use

Refer to "Safety Concept" booklet

Never use M series microscopes or their components for surgical procedures (e.g. on the eye) unless they are specifically intended for that purpose.

The instruments and accessories described in this operating manual have been tested for safety and potential hazards. The responsible Leica affiliate must be consulted whenever the instrument is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual.

Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims!

Place of use

Refer to "Safety Concept" booklet

Set up the electrical components at least 10 cm from walls and combustible objects.

Avoid large temperature fluctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images.

In warm and warm-damp climatic zones, the individual components require special care in order to prevent the buildup of fungus.

Responsibilities of person in charge of instrument

Refer to "Safety Concept" booklet

Ensure that:

The M series stereomicroscopes and accessories are operated, maintained and repaired by authorized and trained personnel only.

All operators have read, understood and observe this User Manual, and particularly the safety regulations.

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Safety Regulations (cont'd.)

Repairs, service work	Installation in third-party products	assignments and work flow (changing tasks
Refer to "Safety Concept" booklet	Refer to "Safety Concept" booklet	frequently), thorough training of the personnel,
		giving consideration to ergonomic and organi-
Only original Leica Microsystems spare	Disposal	zational aspects.
parts may be used.	Refer to "Safety Concept" booklet	The ergonomic design and construction of the
		Leica M stereomicroscopy series are intended to
Before opening the instruments, switch off	Legal requirements	reduce the exertion of the user to a minimum.
the power and unplug the power cable.	Refer to "Safety Concept" booklet
		Direct contact with eyepieces can be a potential
Touching the live circuit can cause injury.	EC Declaration of Conformity	transmission path for bacterial and viral infec-
	Refer to "Safety Concept" booklet	tions of the eye.
Transport		The risk can be kept to a minimum by using
Use the original packaging for shipping or	Health risks	personal eyepieces for each individual or
transporting the individual modules of the	Workplaces equipped with stereomicroscopes	detachable eyecups.
Leica M stereomicroscopy series and the	facilitate and improve the viewing task, but
accessory components.	they also impose high demands on the eyes
	and holding muscles of the user. Depending
In order to prevent damage from vibrations,	on the duration of uninterrupted work, asthe-
disassemble all moving parts that (accord-	nopia and musculoskeletal problems may
ing to the user manual) can be assembled	occur. For this reason, appropriate measures
and disassembled by the customer and	for reduction of the workload must be taken:
pack them separately.	optimal arrangement of workplace, work

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Safety Regulations (cont'd.)

Light sources: safety regulations

Protective measures of the manufacturer:

UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays.

Dummy filter carriers in the free positions of the rapid filter changer prevent direct UV radiation from reaching the eyes.

UV filters are installed in the observation beam paths to protect the eyes.

The stray-light protection on the lamp housing prevents irradiation of the hands.

Warning

UV radiation could damage the eyes. ­Therefore:

Never look into the light spot on the specimen plane without a UV protection screen.

Never look into the eyepieces if no excitation filter is in the beam path.

Fill empty filter positions with dummy filter carriers (e.g. M165 FC).

Do not select a white, strongly reflective background for the specimen.

Supply unit

Always unplug the supply unit from the power supply:

When installing and disassembling the lamp housing

Before opening the lamp housing

When replacing the high-pressure mercury lamp and other parts, such as the heatabsorbing filter or the collector

During maintenance work on the supply unit

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Safety Regulations (cont'd.)

Lamp housing

Never open the lamp housing while the lamp is switched on. Risk of explosion, UV exposure, blinding!

Before opening the lamp housing, allow it to cool off for at least 15 minutes. Danger of explosion!

Never cover the air duct on the lamp housing. Danger of fire!

Mercury lamp

Follow the user manual and safety regulations provided by the lamp manufacturer, and, in particular, the section on how to proceed if a lamp breaks and releases mercury.

For transport, remove the mercury lamp, transport it in its original packaging and protect moving parts in the lamp housing using the transport anchors.

When it has reached the end of its rated life (follow the manufacturer's specifications and the minute meter on the supply unit).

To minimize the risk of explosion, replace a discolored mercury lamp promptly.

Leica Microsystems assumes no liability for damage caused by exploding, incorrectly installed or improperly used mercury lamps.

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Table of Contents

Inhaltsverzeichnis

General Instructions	2
Important Safety Notes	3
Symbols Used	4
Safety Regulations	5
Safety Regulations (cont'd.)	6
Safety Regulations (cont'd.)	7
Safety Regulations (cont'd.)	8
M series
Congratulations!	12
The Modular Design: Everything is Relative	13
Maximum Compatibility	14
On We Go	15
Assembly
Base and Focusing Column	17
Microscope Carrier	18
Optics Carrier	19
Tube	20
Eyepieces	21
Objective	22
Leica LED5000 CXI (Coax Illumination)	23

Leica LED5000 CXI (cont'd.)	24
Leica LED3000 NVI™ (Near Vertical Illumination)	25
Leica LED3000 NVI™ (cont'd.)	26
Quick Start Guide
The Reliable Route to Success	28
Overview of an M Series Microscope	29
The Correct Interpupillary Distance	30
Using the Eyepieces	31
Focusing	32
Adjusting the Resistance of the Focus Drive	33
Changing the Magnification (Zoom)	34
Ratchet Steps and Magnification Levels	35
Eyepieces & Tubes
Magnification Factor of the Eyepieces	37
Health Notes	38
Dioptric Correction	39
Dioptric Correction and Parfocality	40
Graticules	41
ErgoTube 10° to 50°	42

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Photography & Video
Photography & Video	44
Photo Tubes and C-mounts	45
Video/Phototubes HD F & HD V	46
Optical Accessories
The Different Types of Objectives	48
System Illumination
Leica LED3000 NVI™ (Near Vertical Illumination)	50
Leica LED5000 CXI (Coax Illumination)	51
Dimensional Drawings
Leica M50	53
Leica M50	54
Leica M50	55
Leica M60	56
Leica M80	57
Leica M80	58
Leica M80	59
Leica M80	60
Leica M80	61

Appendix
Calculating the Total Magnification and Field of View Diameter		63
Care, Maintenance, Contact Persons		64
Care, Maintenance, Contact Persons (cont'd.)		65

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The Leica M series

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Congratulations!

The New Leica M Series

The introduction of the Leica M50, M60 and M80 adds three new highquality routine stereomicroscopes to the proven M-Series range from Leica ­Microsystems. The optical brilliance and the wide range of accessories make them the ideal microscope for quality assurance and similar industrial applications.

Magnification in stages or zoom

The Leica M50 stereomicroscope includes precisely reproducible magnification levels for repeated examinations, measurements, drawing or photography of objects under absolutely identical conditions. The five easily selectable positions can be set without removing the eyes from the eyepieces. This ensures that your results remain comparable at all times without great efforts.

The Leica M60 and M80 stereomicroscopes can be used for a wide range of routine applications with the 6:1 / 8:1 zoom and switchable ratchet steps. The large working­ distance and the brilliant imaging power shows you the finest details of your specimens without losing your overview of large workpieces.

Common to all microscopes is the Leica range of accessories. Whether you need a variety of illumination types, a wide selection of objectives or the Leica swinging-arm system – there is a solution for everything there!

Do you already own stereomicroscopic equipment and are thinking of switching to Leica? No problem! Leica M50, M60 and M80 fit into microscope carrier with a diameter of 76 mm and are therefore compatible with many suppliers. You can simply continue to use your components and add the proven Leica stereomicroscopes.

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The Modular Design: Everything is Relative

The Leica M series provides maximum flexibility in choosing equipment, thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades. The optics carriers, eyepieces, bases and more can be combined in any way you choose, allowing you to create the microscope that best suits your needs.

Despite this, you will notice that the controls and individual components do not differ significantly. Whichever configuration you choose, you will quickly feel right at home.

Have a special request? Let us know!

Leica Microsystems enjoys an exceptional reputation when it comes to devising customer-

specific solutions. If you have a special request that cannot be met with standard parts, contact your Leica consultant. We have a solution for every problem.

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Maximum Compatibility

Leica engineers were careful to ensure that the new Leica M series—like its predecessors— remains compatible with existing series. This means that objectives, bases, tubes and so on can be reused.

Objectives

All objectives of this new generation have the same reference focal length as the objectives of the MZ generation. If you prefer, you can therefore continue to use the previous Leica objective series.

Tubes

The interface between the optics carrier and the tube has remained the same, so existing tubes fit the new M series. The new tubes are designed for eyepieces with field number 23, while the predecessor models were only designed for field number 21, resulting in a smaller object field.

Eyepieces

The new M-series eyepieces have an audible and tangible click to provide immediate feedback in case of accidental adjustment.

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On We Go

If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on Page 29.

If, on the other hand, you are assembling the microscope yourself, continue with the "Assembly" chapter, which begins on Page 16.

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Assembly

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Base and Focusing Column

The first step is to connect the focusing column	Assembling the column adapter	2. Install the base on the column using the
of the M series to the corresponding base.	1. Remove the three screws provided from the	three screws.
	column.
Tools used
Hex socket screwdriver, 3 mm

Tip: Place the column on the edge of a table to attach the base and have another person help you.

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Microscope Carrier

Tools	Alternative installation options
Allen screwdriver provided	Depending on the desired working distance, the
	microscope carrier can be installed in the stan-
	dard way or upside down (see figure below).
Assembly
The microscope carrier is fastened to the column
using the screw provided.

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Optics Carrier

Tools used	Assembling the optics carrier
None	1. Unscrew the clamping screw on the holder
	for the optics carrier.

2. Place the optics carrier into the holder and align it in such a way that the notch in the optics carrier and the bottom clamping screw are on top of each other.

2.Tighten the clamping screw below the optics carrier.

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Tube

All intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner.

Tools used

No tools required.

Preparation

1.Unscrew the positioning screw and remove the protective cover.

Assembling the tube

2.Push the tube (for example, the inclined binocular tube) into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove.

3. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position­ .

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