Leica M125 C, M165 C, M205 C, M205 A User Manual
Leica M125 C
Leica M165 C
Leica M205 C
Leica M205 A
User Manual
Leica M Series User Manual 2
General Instructions
Safety concept
Before using your microscope for the first
time, please read the "Safety concept" booklet
included with your instrument. It contains additional information about handling and care.
Use in clean rooms
The Leica M series can be used in clean rooms
without any problems.
Cleaning
O Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
O Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and specimens could be contaminated by
abraded particles.
O In most cases, we can provide special solu-
tions on request. Some products can be
modified, and we can offer other accessories for use in clean rooms.
Servicing
O Repairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
O Ensure that the Leica stereo microscope
is operated, maintained and repaired by
authorized and trained personnel only.
Leica M Series User Manual 3
Safety Concept
The individual modules of the Leica M stereo
microscopy series include an interactive
CD-ROM with all relevant user manuals in 20
other languages. Keep it in a safe place, and
readily accessible to the user. User manuals and updates are also available for you
to download and print from our web site at
www.stereomicroscopy.com.
This operating manual describes the special
functions of the individual modules of the Leica
M stereo microscopy series and contains important instructions for their operational safety,
maintenance, and accessories.
The "Safety concept" booklet contains additional safety information regarding the service
work, requirements and the handling of stereo
microscope, accessories and electrical accessories as well as general safety instructions.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Before installing, operating or using the instruments, read the user manuals listed above. In
particular, please observe all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Leica M Series User Manual 4
Symbols Used
Read and understand user manual
before using this device.
Warning of a danger
•
This symbol indicates especially impor-
tant information that must be read and
complied with. Failure to comply can cause the
following:
O Hazards to personnel
O Functional disturbances or damaged
instruments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that, if not observed,
can cause the following:
O Hazards to personnel
O Functional disturbances or damaged
instruments
Danger due to hot surface
This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional information or explanations that intend to
provide clarity.
Action
O This symbol refers to actions described in
the text that are to be carried out.
Figures
(1) Numbers in brackets within the descrip-
tions relate to the figures and the items
within those figures.
Leica M Series User Manual 5
Safety Instructions
Description
The individual modules fulfill the highest
requirements for observation and documentation of Leica stereo microscopes of the Mseries.
Intended use
O Refer to "Safety Concept" booklet
Non-intended use
O Refer to "Safety Concept" booklet
Never use M series microscopes or their components for surgical procedures (e.g. on the eye)
unless they are specifically intended for that
purpose.
The instruments and accessories described in
this operating manual have been tested for
safety and potential hazards. The responsible
Leica affiliate must be consulted whenever
the instrument is altered, modified or used in
conjunction with non-Leica components that
are outside of the scope of this manual.
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims.
Place of use
O Refer to "Safety Concept" booklet
O Electrical components must be placed at
least 10 cm away from the wall and from
flammable substances.
O Avoid large temperature fluctuations,
direct sunlight and vibrations. These conditions can distort measurements and micrographic images.
O In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.
Responsibilities of person in charge of
instrument
O Refer to "Safety Concept" booklet
Ensure that:
O The M series stereo microscopes and
accessories are operated, maintained and
repaired by authorized and trained personnel only.
O All operators have read, understood and
observe this User Manual, and particularly
the safety instructions.
Leica M Series User Manual 6
Safety Instructions (Continued)
Repairs, service work
O Refer to "Safety Concept" booklet
O Only original Leica Microsystems spare
parts may be used.
O Before opening the instruments, switch off
the power and unplug the power cable.
Touching the live circuit can cause
injury.
Transport
O Use the original packaging for shipping or
transporting the individual modules of the
Leica M stereo microscopy series and the
accessory components.
O In order to prevent damage from vibrations,
disassemble all moving parts that (according to the user manual) can be assembled
and disassembled by the customer and
pack them separately.
Integration in third-party products
O Refer to "Safety Concept" booklet
Disposal
O Refer to "Safety Concept" booklet
Legal requirements
O Refer to "Safety Concept" booklet
EC Declaration of Conformity
O Refer to "Safety Concept" booklet
Health risks
•
Workplaces with stereo microscopes
facilitate and improve the viewing task,
but they also impose high demands on the eyes
and holding muscles of the user. Depending on
the duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
O Optimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently).
O Thorough training of the personnel, giving
consideration to ergonomic and organizational aspects.
O The ergonomic design and construction of
the Leica M stereo microscopy series are
intended to reduce the exertion of the user
to a minimum.
Leica M Series User Manual 7
Safety Instructions (Continued)
•
Direct contact with eyepieces is a
potential transmission method for
bacterial and viral infections of the eye.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.
Technical safety
To connect the Leica M Series microscopes to
power, use a power cable that meets the power
cable specifications in your country. The power
socket used has to be equipped with a 16 A or
10 A fuse. Only connect the instrument to a
grounded power socket that has been installed
according to regulations. The power supply
voltage has to correspond to the voltage specified on the identification label. Make sure that
the ground connection is neither defective
nor interrupted, since otherwise a malfunction
poses a risk of fatal injury. Never unplug the
power plug with moist or wet hands. There is
danger of an electric shock.
Instrument safety and EMC
Our device has been designed, produced and
tested in compliance with
O IEC EN 61010-1: Electrical equipment for
measurement, control and laboratory use
– Safety – Part one: general requirements
O EN 60950-1: Information technology
equipment – Safety – Part one: general
requirements
O Radio interference suppression in compli-
ance with EN 55011 class B
O Noise immunity in compliance with
DIN EN 61326-1.
The device meets the requirements of EU
directives
O 2014/35/EU Low voltage directive
O 2014/30/EU EMC directive
O 2011/65/EU RoHS directive
and carries the CE mark.
The device must be disposed of in
accordance with 2012/19/EU WEEE
directive.
Intended for indoor use only in all EU member
states, EFTA states and Switzerland.
Leica M Series User Manual 8
Safety Instructions (Continued)
Handling electrical components
Never install any other plug (NEMA
5-15P) or unscrew any mechanical
components unless expressly instructed to do
so in the instructions.
The microscope must be connected to a
grounded outlet.
Always position the microscope so that
you can disconnect it from the power
supply at any time. The power cable is provided
as the power disconnect device.
Leica M Series User Manual 9
Contents
Leica IsoPro Manual XY Stage: Assembly 31
Cables: Connections 34
Cables: Cable Duct 35
Cables: Diagram 36
Leica LED5000 MCI 37
Leica LED5000 MCI: Alternative Assembly 38
Leica LED5000 RL: Assembly 39
Leica LED5000 RL: Installing Optional Accessories 41
Leica LED5000 RL: Use 42
Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes
(Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A) 44
Leica LED5000 NVI: Installing Optional Accessories 47
Quick Start Guide
The Fastest Route to Success 50
Overview of an M Series Microscope 51
The Correct Interpupillary Distance 52
Using the Eyepieces 53
Focusing 54
Adjusting the Resistance of the Focus Drive 55
Changing the Magnification (Zoom) 56
Ratchet Steps and Magnification Levels 57
Parfocality: More Comfort and Convenience for Your Work 58
Iris Diaphragm 59
General Instructions 2
Safety Concept 3
Symbols Used 4
Safety Instructions 5
Congratulations! 11
A Step Towards Infinity 12
The Electronics: Comfort, Convenience and Safety for Your Experiments 13
The Modular Design: Everything is Relative 14
Maximum Compatibility 15
On We Go 16
Assembly
Base and Focusing Column 18
Assembling the Focusing Column for TL Bases 19
Assembling the Focusing Column With an Incident-light Base 20
Optics Carrier 21
Tube 22
Eyepieces 23
Objective 24
Objective Nosepiece – Assembly 25
Objective Nosepiece – Adjusting Parfocality 26
AX Carrier – Preparation 27
AX Carrier – Assembly 28
Transmitted-light Base Leica TL BFDF: Before First Use 29
Transmitted-light Base Leica TL BFDF 30
Leica M Series User Manual 10
Eyepieces
Magnification Factors of the Eyepieces 61
Health Notes 62
Dioptric Correction 63
Dioptric Correction and Parfocality 64
Graticules 65
Photography & Video
Photography & Video 67
Photo Tubes and C-mounts 68
Trinocular Video/Phototube 50 % 69
Trinocular Video/Phototube 100 % 70
Trinocular Video/Phototube 100 %: ErgoTube 5°– 45° 71
Microscope Carrier
The Microscope Carrier AX 73
The Objective Nosepiece 75
Objectives and Optical Accessories
The Different Types of Objectives 77
Bases
Transmitted-light Base Leica TL BFDF: Controls 79
Transmitted-light Base Leica TL BFDF: Operation 80
Leica IsoPro (Non-motorized): Controls 81
System Illumination
Leica LED5000 MCI 83
Leica LED5000 RL 85
Accessories
Leica Hand Wheel and Foot Switch 87
Leica SmartTouch 88
Dimensional Drawings
Leica M125 C / Leica M165 C 90
Leica LED5000 MCI 92
Leica LED5000 RL 93
Leica LED5000 NVI 94
Leica MATS TPX 95
Specifications
Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A 97
Transmitted-light Base Leica TL BFDF 101
Motorized Focus Drives With "Zoom" Carrier
(M125 C, M165 C, M205 C, M205 A) 102
Appendix
Calculating the Total Magnification and Field of View Diameter 104
Care, Maintenance, Contact Persons 105
Contents (Continued)
Leica M Series User Manual 11
Congratulations!
Congratulations on obtaining your new Leica M series stereo microscope.
We are convinced it will exceed your expectations, as never before have
we applied our decades of experience in the areas of optics, mechanical
engineering and ergonomics in such an uncompromising manner.
The M series embodies all the qualities you associate with the name Leica
Microsystems: excellent objectives, high-quality engineering, and reliability. Furthermore, the modular design ensures that the M series adapts
perfectly to your needs – no matter which accessories you require for your
tasks.
Though the reliability and robustness of Leica stereo microscopes is
legendary, like any high-tech product, the M series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereo microscope will continue
to work as smoothly and reliably as on the very first day.
We wish you the best of success in your work – after all, you are now
equipped with the best tool!
Leica M Series User Manual 12
A Step Towards Infinity
Ever since their introduction by Horatio S.
Greenough, stereo microscopes have worked
according to the optical principles based
primarily on Ernst Abbe's research. For over a
century, ingenious optics designers and engineers have worked to push magnification, resolution and image fidelity to the limit permitted
by optics.
In doing so, they have always been constrained
by the interrelation between three factors: the
higher a microscope's resolution, the lower the
available working distance. If one increases the
distance of the optical axes, the three-dimensional image seen by the observer becomes
distorted – a sphere becomes an ellipse, a flat
surface curves toward the observer.
Limits are made to be broken.
The Leica M205 C is the world's first stereo
microscope with a zoom range of 20.5:1. This
accomplishment, however, was not enough for
Leica's engineers. With the new FusionOptic in
the M205 C, they have succeeded in going yet
another step beyond previous limits. In addition
to the increase in magnification, the resolution,
too, has been increased to up to 1,050 lp / mm*,
which corresponds to a resolved structure size
of 472 nm.
Of course, this performance increase benefits
your everyday work. Set up your specimens
on the microscope with comfortable freedom
of movement and discover details in stereo
microscopy that you could never see before.
* Leica M205 C with 1.0× planapochromat and 10× eyepieces
Leica M Series User Manual 13
The Electronics: Comfort, Convenience and Safety for Your Experiments
Never before have electronics been used as
extensively in a Leica series as in the new
M series. Optics carrier, tripod, base and illuminator are all connected using electrical contacts –
which provides a number of advantages.
Reliability for your experiments
Exact reproducibility of results is an increasingly common demand, particularly in research
and development. The continuous encoding
captures parameters such as the magnification, the illumination, the position of the iris
diaphragm and more to the Leica LAS X software. Thus you always know the conditions
under which an image was acquired.
Fewer cables
A large part of the cables have been routed in
the interior of the column. Data are also transferred via the interface between the column,
the optics carrier and the carrier. The direct
result is that you need fewer cables – this not
only makes your workstation neater and more
comfortable, it even makes it appear larger.
Contacts not only transmit data, but also supply
the power.
Leica Application Suite X (LAS X) evaluates the
transmitted data and can restore the test
situation later.
Leica M Series User Manual 14
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional reputation when it comes to devising customerspecific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. We have a solution for
every problem.
The Modular Design: Everything is Relative
The Leica M series provides maximum flexibility in choosing equipment, thanks primarily to
the modular configuration and the compatibility that Leica has painstakingly maintained for
decades. The optics carriers, eyepieces, bases
and more can be combined in any way you
choose, allowing you to create the microscope
that best suits your needs.
Despite this, you will notice that the controls
and individual components do not differ significantly. Whichever configuration you choose,
you will quickly feel right at home.
Leica M Series User Manual 15
Leica engineers were careful to ensure that the
new M series – like its predecessors – remains
compatible with existing series. This means
that objectives, bases, tubes and so on can be
reused.
Objectives
All new objectives of the M series are parfocal,
meaning that when used with the objective
nosepiece, they can be replaced while keeping
the specimen in perfect focus.
If you prefer, you can continue to use the previous objective series. In this case, parfocality is
no longer guaranteed.
Tubes
The interface between the optics carrier and
the tube has remained the same, so existing tubes fit the new M series. The new tubes
are designed for eyepieces with field number
23,
while the predecessor models were only
designed for field number 21, resulting in a
smaller object field.
Eyepieces
However, the new M-series eyepieces models
have an audible and tangible click to provide
immediate feedback in case of accidental
adjustment.
Maximum Compatibility
Leica M Series User Manual 16
On We Go
If your new Leica microscope has already been
assembled and commissioned by your Leica
consultant, click here to skip through the installation instructions and go directly to the Quick
Start Guide on page 49.
If, on the other hand, you are assembling
the microscope yourself, continue with the
"Assembly" chapter, which begins on page 17.
Leica M Series User Manual Assembly 17
Assembly
Leica M Series User Manual Assembly 18
The first step is to connect the focusing column
of the M series to the corresponding base.
Tools used
O Hex socket screwdriver, 3 mm
Assembling the column adapter
1. Securely install the column adapter on the
column using the four included screws.
Assembling the focusing drive
2. Securely screw the focusing column to the
base using the six included screws.
Base and Focusing Column
Leica M Series User Manual Assembly 19
The first step is to connect the focusing column
of the M series to the corresponding base.
Tools used
O Hex socket screwdriver, 3 mm
Assembling the column adapter
1. Securely install the column adapter on the
column using the four included screws.
Assembling the focusing column
2. Securely screw the focusing column to the
base using the six included screws.
Assembling the Focusing Column for TL Bases
Leica M Series User Manual Assembly 20
Assembling the Focusing Column With an Incident-light Base
When using an incident-light base, the focusing column and motorized focus are installed
directly on the base; no extension plate is
required.
Tools used
O Hex socket screwdriver, 3 mm
Assembly
1. Place the focusing column on the side.
2. Insert the four screws provided into the
outer holes of the base.
3. Screw the base securely onto the focusing
drive.
Leica M Series User Manual Assembly 21
Tools used
O Hex socket screwdriver, 4 mm
Set the on/off switch to "0" to switch off
the device.
Assembling the optics carrier
1. Place the optics carrier on the focusing
column so that the screw fit into the thread
provided and the lug fits into the groove.
2. Press the optics carrier backwards to the
focusing column and screw it in place using
your other hand.
Optics Carrier
Leica M Series User Manual Assembly 22
Tube
All intermediate tubes that fit between the
optics carrier and the binocular tube are fitted
in the same manner.
Tools used
O No tools required.
Preparations
1. Unscrew the positioning screw and remove
the protective cover.
Assembling the tube
2. Push the tube (for example, the inclined
binocular tube) into the dovetail ring and
rotate it slightly in both directions until the
positioning screw meshes with the guide
groove.
3. While holding the tube only slightly, carefully tighten the positioning screw. It
is automatically brought to the correct
position.
Leica M Series User Manual Assembly 23
Eyepieces
Tools used
O No tools required.
Magnification range
You can extend the overall magnification range
using available 10×, 16×, 25× and 40× widefield eyepieces for persons wearing glasses.
Preparation
1. If you want to use an optional graticule,
insert it now (page 65).
2. Remove the plastic tube guard.
Inserting the eyepieces
3. Push the eyepieces into the tubes as far as
they will go and check to ensure that they
fit tightly and accurately.
4. Securely tighten the clamping screws.
Leica M Series User Manual Assembly 24
Objective
Tools used
O No tools required.
Preparation
1. Remove the protective cap on the optics
carrier by turning it.
Attaching the objective
•
Hold the objective firmly during assem-
bly and disassembly so that it does not
fall onto the stage plate. This applies particularly to the 2× planapochromatic objective,
which is very heavy. Remove all specimens from
the stage plate first.
2. Screw the objective clockwise into the
optics carrier.
Alternative fastening options
O If using the objective nosepiece, read the
instructions on page 25.
O If using the AX carrier, read the instructions
on page 27.
Leica M Series User Manual Assembly 25
Preparations
•
Hold the objectives firmly during
assembly and disassembly so that they
do not fall onto the stage plate.
O Move the drive housing all the way upwards
and remove the optics carrier, if the carrier
has already been installed.
Assembly
1. Remove the transport anchor from the
objective nosepiece.
2. Rotate the moving part by 90° and attach
the objective nosepiece to the drive housing from the front (!). Screw the objective
nosepiece firmly into place.
3. Unscrew the three Phillips screws on the
objective mount of the optics carrier and
remove the intermediate ring.
4. Screw the optics carrier onto the objective
nosepiece.
5. Screw both objectives onto the objective
nosepiece. It makes no difference which
position an objective occupies.
6. Unscrew the locking screws on both sides
of the objective nosepiece.
You can now adjust the parfocality (see instructions on the next page).
Objective Nosepiece – Assembly
Leica M Series User Manual Assembly 26
Objective Nosepiece – Adjusting Parfocality
The following procedure only has to be carried
out once. Afterwards, both objectives are parfocal, meaning that the specimen remains in
focus when the objective nosepiece is rotated.
This procedure must be repeated if you replace
either of the two objectives with another.
The following example assumes the combination of the 1× and 2× planapochromats. If
you are using another objective combination,
replace the 2× objective in the description with
the objective with the stronger magnification.
Preparation
O Open the iris diaphragm.
O Set the dioptric correction of the eyepieces
to "0".
Adjustment
1. Rotate the 2× objective into the beam path
and set it to the lowest magnification.
2. Focus on the specimen.
3. Rotate the 1× objective into the beam path.
4. Turn the objective on the thread in both
directions until the specimen appears
sharp.
5. Toggle to the 2× objective.
6. Select the strongest magnification and
refocus until the specimen appears absolutely sharp.
7. Toggle to the 1× objective.
8. Turn the objective on the thread in both
directions until the specimen appears
absolutely sharp.
By means of zooming, check that the behavior
of the objective is parfocal. Repeat the check
with the other objective. If it is not parfocal,
repeat the procedure.
9. Tighten the locking screws.
Leica M Series User Manual Assembly 27
Tools
O Hex socket screwdriver, 2.5 mm
Preparing the AX carrier
Before the AX carrier is installed, it may first
have to be prepared for the optics carrier to
be used (Leica M125 C, M165 C, and M205 C /
M205 A).
1. Check the switch position.
O If the switch position corresponds to the
optics carrier used, skip the following steps.
Otherwise, proceed as follows:
2. Unscrew the two screws that hold the
switch in position.
3. Pull out the switch, rotate it into the desired
position and reinsert it.
4. Important: push the switch all the way into
the mount while reinserting the screws.
Only this ensures that the switch is positioned correctly.
AX Carrier – Preparation
Leica M Series User Manual Assembly 28
Tools
O Hex socket screwdriver, 4 mm
O Philips screwdriver
Once the AX carrier has prepared for the corresponding optics carrier, it can be installed.
1. Move the slide of the AX carrier into mid
position.
2. Install the AX carrier on the focusing
column.
3. Screw the objective to the AX carrier.
4. Unscrew the three holding screws and
remove the adapter ring on the optics
carrier.
5. Install the optics carrier on the AX carrier.
AX Carrier – Assembly
Wrong Right
Leica M Series User Manual Assembly 29
Removing the transport anchors
•
Before you can use the transmitted-
light base for the first time, it is absolutely necessary to remove the two transport
anchors as follows.
Transmitted-light Base Leica TL BFDF: Before First Use
Anchor of switching slide
Anchor of mirror
Leica M Series User Manual Assembly 30
Transmitted-light Base Leica TL BFDF
Standard delivery
The base is delivered with the adapter plate
installed. The selected stage (XY stage or
standard stage 10 450 562), and the focusing
drive will have to be mounted later.
Stage assembly
The TL BFDF transmitted-light base can be
equipped with different stages.
O Leica IsoPro manual XY stage
O Standard stage 10 450 562
The selected stage is mounted on the base
before commissioning. You can switch between
the stages at any time with just a few hand
movements.
The following paragraph assumes use of the
base without the stage mounted. Disassembly
is performed in reverse order of the following
steps.
Standard stage
1. Take the glass plate from the rectangular
gap in the standard stage.
2. Position the stage on the transmitted-light
base in such way that the four holes align
over those in the base.
3. Attach the stage to the base using the four
Allen screws provided.
4. Insert the glass plate back into the standard
stage.
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