Leica DM1000, DM2000, DM2500, DM3000LED User Manual

Leica DM1000–3000

Technical Information

Microscopy Tailored to You!

MYcroscopy: Designed to adapt to an individual user’s daily routines

Leica Microsystems presents microscopes that are the best in their
class, the Leica DM Digital Microscope Series. Leica has turned
innovative ideas into reality to create a microscope that seamlessly
adapts to the physique and workflow of every user. With the Leica
DM Series, work at the microscope is not only efficient, but also
comfortable.

Leica DM Microscopes are completely adjustable. With just a few
hand movements, the microscope easily adapts to individual pref­erences and work routines. In this way, effortless work combines
with high-performance, and the microscope meets the user’s
needs down to the smallest detail. The Leica DM Series is one of
the most ergonomic microscope lines on the market today – and
the first choice for fatigue-free work; fast, reliable results; and
excellent cost-effectiveness – the benefits of a microscope tai­lored to individual user routines.

MYcroscopy:

Leica DM Microscopes are designed for applications in medicine,
clinical laboratories, pathology, and biology; from routine tasks to
research.

• The Leica DM1000 provides comfort as well as optical brilliance,
and is ideal for the clinical laboratory, in particular histology and
cytology applications.

• The Leica DM1000 LED provides additional convenience with con­stant-color LED illumination and an optional, portable power supply.

• In addition, the Leica DM2000 features a sophisticated focus mech­anism with five focus functions – either 2-gear or 3-gear focusing,
with torque adjustment and adjustable stage height stop.

• The Leica DM2500 also offers powerful 100 W illumination and is
well-suited for pathology and biomedical research that require
specialized contrast methods such as differential interference
contrast (DIC).

• The “intelligent automation” of the Leica DM3000 supports
greater intuitive efficiency and enhanced user comfort. The
microscope is designed for clinical laboratories, all biomedical
routine and research applications, and is ideal for pathology
applications where the user spends hours at a time at the micro­scope.

Configured for specific and universal applications

Cytology

High-performance HI PLAN 10x CY SL screening objec­tive, light intensity synchronized with HI PLAN 40x.

Pathology

Ultra-hard ceramic stage surface ensures long product
life. The 1.25x objective provides a clear overview.

Hematology

Optionally, all five versions can be equipped with fluorescence
contrast. The Leica DM Digital Microscope Series can be uniquely
tailored to specific laboratory applications.

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Easy, single-handed operation (for x/y/z) when simulta­neous counting or written notation is required.

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Effortless Microscopy Means Comfort

The Leica DM Digital Microscope Series makes routine work as comfortable and effortless
as possible. Only a few hand movements are required to adjust the microscope to the user’s
seated height, head posture, arm length, and hand size. This adaptability provides reliable
protection from muscular tension, poor posture, and long term, detrimental health effects.
Microscopy has never been more comfortable and pleasant.

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1. Height-adjustable
Focus Knobs

A technological first!

No two hands are alike. And so the Leica
DM Series enables every hand to rest on the
focus knobs in complete comfort. The focus
knobs can be precisely adjusted to fit the
user’s hand. This prevents hand, arm, and
shoulder tension and ensures a comfort­able, fatigue-free grip – without additional
arm supports.

2. Adjustable Eyetubes

Relaxed head posture eases tension

Proper arrangement of the eyetubes pro­motes good posture while sitting at the
microscope. The Leica DM Series offers a
wide range of products to customize the
eyetube settings. Choose from an
ergonomic viewing angle of 15° or ad­justable variotubes for a relaxed head posi­tion while viewing. Customized eyetube
positioning prevents neck and back muscle
strain, and shoulder and lower neck muscle
tension, even over lengthy work sessions.
Leica’s optional ErgoLift even adjusts the
viewing height and angle of the entire
microscope stand to adapt to the user’s
body.

3 True Symmetrical

Operation

Comfortable shoulder posture provides comfort

The stage drive and focus knob are
arranged at the same height on the micro­scope, forming a straight line and positioned
at an equal distance from the user. Because
of this symmetry both hands are comfortably
aligned during work, and the shoulders
automatically assume a right-angle position
to the body’s axis. The result: the user sits at
the microscope in a straight, relaxed pos­ture.

4 Fast Changeover from

Right- to Left-handed
Operation

One-of-a-kind!

The user can set up Leica DM Microscopes
for either right- or left-handed operation as
the default. This feature is especially bene­ficial when alternating users on one micro­scope.

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5. Unique

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7. Ergonomic

Ultra-hard Surface

Designed to last a lifetime

The microscope’s stage surface is made of a
new, hard ceramic that makes the stage
resistant to scratches and chemicals, and is
completely durable for years, even under
the most rigorous usage. The light beige
color of the ceramic provides an ideal sur­face on which to identify specimens by out­line and coloring before placing under the
objective.

6 New Design

More work space

The design of the stage is rounded, and the
entire microscope is compactly designed,
with no protruding parts. The compact
design frees additional work space around
the microscope.

Control Knobs

Comfort is at hand

As an alternative to the standard knobs,
Leica’s rubberized ErgoKnobs are available
to provide a comfortable, secure grip for the
focus drive. For the x-y drive knobs, replace­able sleeves made of soft, yet firm rubber
are included with the stage drive. Simply
pull the sleeves over the knobs to enhance
the feeling of sensitive control.

8 Comfortable ErgoLift

Variable height adjustment

Leica’s optional ErgoLift is a special,
adjustable plate that raises or lowers the
entire microscope to the seated height of
each user with just a few hand movements.

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Differential
Interference Contrast Fluorescence

Optical contrast methods such as Darkfield, Polarization,
Phase or DIC provide brilliant images. Ready for a universal
condenser and any 6- or 7-position objective turret.

High-intensity fluorescence with zero pixel shift for all kinds
of probes and labels. Five positions are available.

Histochemistry

Best contrast for viewing autoimmune
reactions Brightness can be reduced with
the integrated N4 filter.

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Elegant Design for Efficient,
High-quality Work

Better performance, higher throughput, and faster workflow: three important benefits that pro­vide maximum efficiency to today’s laboratories. Microscopes with well-engineered functions
down to the smallest hand movements allow users to work quickly and smoothly. The Leica DM
Digital Microscopes are thoughtfully and elegantly designed to provide these benefits.

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9. Simultaneous Focus and
Stage Control

One-handed adjustment

The focus and stage can be adjusted with
just one hand to allow faster, more efficient
workflow. While one hand (left or right)
focuses the specimen, the other hand is free
for other tasks, such as note taking.

10. New Slide Holders

Ergonomic design means efficient work

Leica’s new slide holders help make micro­scope work easier. The slide holders are
constructed so that specimens can be
changed with one hand – and one hand
movement. The specimen is inserted into
the holder and removed from it using just
two fingers. This ensures a fast, fluid work­flow.

11. Premiere: Color-coded
Diaphragm Settings

Faster identification and adjustment

The aperture diaphragm’s scale (on the con­denser) features color-coded markings,
which correspond to the standardized color
codes of the objectives. The user can iden­tify, at a glance, the diaphragm that best
matches the currently selected objective,
and then quickly and correctly configure
the settings.

12. Fast

Lamp Replacement

Efficient maintenance

The lamps can be replaced easily and in
very little time. Just remove the socket from
the side of the microscope, replace the
lamp, and reinsert the socket into the micro­scope – and the task is done. The micro­scope does not need to be moved to replace
the lamp.

Another option: the new Leica DM1000 LED
with long-lasting LED illumination means
lamp changes are a thing of the past.

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Excellent Image Quality: Optical Brilliance

Leica is the well-known brand for excellent optics. Users can expect Leica’s renowned excellent
image quality and exceptional viewing comfort. Leica DM Microscopes feature a host of innovative

functions that enable the user to quickly and reliably configure the optical settings for the best viewing
conditions. Leica’s objectives provide brilliant, high-contrast images. The optical system is designed to
reduce eyestrain and ensure fatigue-free work at the microscope.

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13 Leica’s Renowned,

High-quality Optics

The best brightness, brilliance, contrast

The Leica DM Series features spectacular
optical quality. Brilliantly detailed images
with razor-sharp contrast await the viewer.
Leica’s high-quality optics clearly reveal the
finest cellular structures, which makes
identification easy. Leica’s HI PLAN plana­chromat objectives offer improved field-flat­tening and color correction. The HI PLAN
10x objective is particularly well-suited for
cytology applications. Its long working dis­tance of 12mm allows specimens to be
marked at 10x magnification.

14. Brightness-matched
Objectives

No need to adjust light intensity

Leica’s new HI PLAN SL (Synchronized
Light) objective series with 4x, 10x, and 40x
magnification is particularly easy on the
eyes. Leica’s SL objectives are matched to
each other so that brightness always
remains constant for the user, regardless of
the selected magnification. This eliminates
the need to continuously adjust the bright­ness and reduces the eyestrain that can
occur due to large fluctuations in light inten­sity. The preferred color impression remains
the same for each objective.

15. Planachromat Objective

Excellent field-flattening and color correction

Leica’s special Planachromat HI PLAN CY
10x/0.25 objective provides excellent field­flattening and color correction, and offers a
long working distance of 12mm for clinical
applications. This objective is also available
in the SL version.

16. Overview Objective

1.25x objective – for screening

Specifically designed for the clinical labora­tory, Leica offers a screening objective with

1.25x magnification. Specimens can be sur­veyed and recorded quickly and easily.

17. The New
Fluorescence Axis

Five filter block positions provide
greater flexibility

Leica’s stable, high-quality fluorescence
axis features five filter block positions.
Users can rapidly switch back and forth
between them. Leica’s extensive range of
filters can be used with a wide variety of flu­orochromes. Leica’s filter blocks feature
"zero pixel shift" technology to prevent the
image from shifting when changing filter
blocks, so that images are correctly super­imposed.

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Intelligent and Innovative:
The Automated Leica DM3000

Even faster, more comfortable, and more efficient: the Leica DM3000 Microscope combines oper­ating convenience with an ergonomic design. The automated Leica DM3000 optimizes work pro­cesses while adapting to each user’s physical requirements. With its unique toggle mode for
changing objectives and automated condenser, the intuitive Leica DM3000 provides the greatest
speed and precision while maintaining reliability for all clinical, as well as routine biomedical and
research applications.

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18 Change Objectives at

the Touch of a Button

Eyes stay focused and hands remain
in place

Leica’s motorized objective turret allows
magnification to be changed in only half a
second. Two buttons, conveniently located
behind the focus knobs, control the turret.
Leica’s unique toggle mode: any two of the six
objectives can be assigned to these buttons.
The remaining four objectives are available at
the touch of a button whenever a different
magnification is required. Six buttons on the
base of the microscope are assigned to the
six objectives. An optional foot pedal is also
available, which frees the user’s hands for
activities such as note taking.

19. Automated
Condenser Head

Intuitive and efficient work

The automated condenser head automati­cally swings out when using objectives with
less than 10x magnification and swings back
into position when a higher magnification is
selected. The microscope knows the best
condenser position for every objective. For
special applications, the user can manually
adjust the condenser position by assigning
this position to a control button. Leica’s
automated condenser head accelerates
workflow in the laboratory.

20. Automatic Light

Intensity

The best light for any magnification

Whenever the objective is changed, the
Leica DM Microscope automatically adjusts
the light intensity to best illuminate a speci­men at any given magnification. The user’s
most recently used light values are stored
for each objective. And the brightness
remains constant, which prevents sudden
light intensity changes that can cause eye­strain.

Adjustment

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Microscopy in a New Light
The Leica DM1000 LED – with Constant-color Illumination
and Optional Solar Panel

The new Leica DM1000 LED is equipped with long-life LED illumination, which replaces the
conventional halogen bulb. Leica also offers a portable, solar-powered version for field­based deployment. Users of the Leica DM1000 LED also benefit from the same ergonomic,
performance-enhancing advantages of the Leica DM1000.

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21. Bulb Replacement
Is History

The LEDs have an extremely long service
life of approximately 100,000 hours. Assum­ing six hours of use per day, the bulb’s ser­vice life is 50 years. This saves laboratories
both time and money.

22. Comfortable,
Pleasant Light

LED illumination provides a cool color
impression. The light contains a relatively
high blue cast, which closely approximates
daylight. The color temperature also
remains constant at different illumination
intensities and is easy on the eyes.

23. Energy Savings

24. Portability

Leica offers an optional, portable power sup­ply for the new Leica DM1000 LED. When
using the microscope for field-based applica­tions, the flexible solar panel delivers power
to the rechargeable battery, which is inte­grated with the stand. The microscope can
then be used for approximately eight hours on
a fully charged battery.

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The Leica DM1000 LED features a 4 W LED
bulb. Because of its low power consump­tion, the system is energy efficient.

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Easy Image Archiving

The Leica Image Organizer with integrated database is an easy-to-use software tool for
image archiving, particularly for clinical applications, and is also ideal for any field in which
simple image storage and search options are required.

Save and Retrieve Data
with Just One Click

With the Leica Image Organizer, a user can
save image information, including micro­scope setting, camera setting, specimen
type, and patient data. All data is linked to
the image and can be retrieved at one click
of a button.

Easy Storage and Fast
Search Options

Images are easily saved under multiple cat­egories using the Leica Image Organizer. A
sophisticated search function utilizes vari­ous search criteria to quickly find images
and associated data.

Overview or Detailed
Image Viewing

The Leica Image Organizer displays images
either in an overview format in the gallery or
as single exposures via the magnification
function. The user can quickly find the
desired images in the gallery. The magnifica­tion function then reveals the image with the
finest details in razor-sharp clarity.

Easy Image Archiving

The Leica Image Organizer features an inte­grated database, which is an easy-to-use
archiving tool that is particularly useful for
clinical laboratory applications. The archiv­ing feature is ideal for any application that
requires simple, easy-to-use image storage
and search options.

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Leica Design by Christophe Apothéloz

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Leica DM1000, Leica DM1000 LED

Leica DM2000, Leica DM2500, Leica DM3000

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Specifications

* New unique features

Stand Power supply External 4 W/90–250 V

Integrated power supply, 30 W stabilized / 90–250 V auto. adjustment
Integrated power supply, 100 W stabilized / 90–250 V auto. adjustment

Koehler Variable Koehler illumination

Prefixed Koehler (option)

Eyetubes Ergotube 15° viewing angle FOV 22*

Standard tube 30° viewing angle FOV 22
Ergo tilting eyetube FOV 25 (long)
Ergo tilting eyetube FOV 22 (short)
Advanced ergo tilting eyetube FOV 22

Phototubes Trinocular ergo tilting phototube 50/50 FOV 22

Trinocular ergo tilting phototube 100/100 FOV 25
Trinocular ergo tilting phototube 50/50 FOV 25
BDT25 0/50/100 dual port option FOV 25
EDT22 50/50 FOV 22

Ergomodules 30 mm

60 mm

Operation

TL axis

Fluo-axis

Focus Height adjustable focus knobs*

2-gear focusing: coarse, fine, focus stop

5 focus functions: 2- or 3-gear focusing, focus-stop,
adjustable torque*

Objective turret 5-fold

6-fold
7-fold

Automatic 6-position objective turret with additional toggle mode*

Objective Brightness synchronized objective series 4x, 10x, 40x*
Light intensity Automatic light intensity adaptation for varying
adjustment magnifications*
Stage Ceramic-coated (ultra hard ceramic)*

Left/right (exchangeable by user)*

Rackless
T

elescopic drive
Adjustable torque
Rotatable / stage for two slides (option)

illumination TL illumination: LED

12 V/30 W halogen lamp, easy bulb-exchange
(with special drawer)*

12 V/100 W halogen lamp with lamp house

Filtering Flip-out blue filter

Filter holder for 2 filters
Filter magazine for 3 filters
Built-in filter magazine for 3 filters

Condenser – Standard condenser CL/PH color coding* (2.5x–100x)

– Achr

. Apl. Flip top condenser color coding* (1.25x–100x)

– Automated Achr. Apl. Flip top condenser

with color coding (1.25x–100x)*

– Universal condenser UCL BF/Phase/DF
– Universal condenser UCA BF/Phase/DF/DIC

Contrast methods BF

DF, PH, POL
DIC

Illumination 50 W Hg

75 W Xe
100 W Hg
12 V/100 W halogen lamp

Filter cube Slider with 3 positions for filter cubes
changer 5-position filter cube turret

DM1000

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DM2500

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DM3000

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“With the user, for the user”
Leica Microsystems

Leica Microsystems operates internationally in four divi­sions, where we rank with the market leaders.

Life Science Division

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The Leica Microsystems Life Science Division supports the
imaging needs of the scientific community with advanced
innovation and technical expertise for the visualization,
measurement, and analysis of microstructures. Our strong
focus on understanding scientific applications puts Leica
Microsystems’ customers at the leading edge of science.

Industry Division

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The Leica Microsystems Industry Division’s focus is to
support customers’ pursuit of the highest quality end result.
Leica Microsystems provide the best and most innovative
imaging systems to see, measure, and analyze the micro­structures in routine and research industrial applications,
materials science, quality control, forensic science inves­tigation, and educational applications.

Biosystems Division

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The Leica Microsystems Biosystems Division brings his­topathology labs and researchers the highest-quality,
most comprehensive product range. From patient to pa­thologist, the range includes the ideal product for each
histology step and high-productivity workflow solutions
for the entire lab. With complete histology systems fea­turing innovative automation and Novocastra™ reagents,
Leica Microsystems creates better patient care through
rapid turnaround, diagnostic confidence, and close cus­tomer collaboration.

Surgical Division

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The Leica Microsystems Surgical Division’s focus is to
partner with and support surgeons and their care of pa­tients with the highest-quality, most innovative surgical
microscope technology today and into the future.

The statement by Ernst Leitz in 1907, “with the user, for the user,” describes the fruitful collaboration
with end users and driving force of innovation at Leica Microsystems. We have developed five
brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to
Science, and Continuous Improvement. For us, living up to these values means: Living up to Life.

Active worldwide

Australia: North Ryde Tel. [phone] Fax [phone]
Austria: Vienna Tel. [phone] Fax [phone]
Belgium: Groot Bijgaarden Tel. [phone] Fax [phone]
Canada: Richmond Hill/Ontario Tel. [phone] Fax [phone]
Denmark: Herlev Tel. [phone] Fax [phone]
France: Rueil-Malmaison Tel. [phone] Fax [phone]
Germany: Wetzlar Tel. [phone] Fax [phone]
Italy: Milan Tel. [phone] Fax [phone]
Japan: Tokyo Tel. [phone] Fax [phone]
Korea: Seoul Tel. [phone] Fax [phone]
Netherlands: Rijswijk Tel. [phone] Fax [phone]
People’s Rep. of China: Hong Kong Tel. [phone] Fax [phone]
Portugal: Lisbon Tel. [phone] Fax [phone]
Singapore Tel. [phone] Fax [phone]
Spain: Barcelona Tel. [phone] Fax [phone]
Sweden: Kista Tel. [phone] Fax [phone]
Switzerland: Heerbrugg Tel. [phone] Fax [phone]
United Kingdom: Milton Keynes Tel. [phone] Fax [phone]
USA: Bannockburn/lllinois Tel. [phone] Fax [phone]

and representatives in more than 100 countries

XII/08/AX/BR.H.

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Part-No. 501-327

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Italian 914 589

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Spanish 914 588

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French 914 587

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German 914 585

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Order nos. of the editions in: English 914 586

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