Leica CM1510-S User Manual
Instruction manual
Leica CM1510 S – Cryostat
V1.4 English – 04/2010
Always keep this manual near the instrument.
Read carefully prior to operating the instrument.
/HLFD&0 6
Cryostat
NOTE
The information, numerical data, notes and
value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we
understand it following thorough investigation
in this field.
We are under no obligation to update the
present manual according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of
this manual.
For erroneous statements, drawings, technical illustrations etc. contained in this manual
we exclude liability as far as permissible according to the national legal system applicable
in each individual case. In particular, no liability whatsoever is accepted for any financial
loss or consequential damage caused by or
related to compliance with statements or other
information in this manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present manual are not to be
considered as warranted characteristics of
our products.
These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is
it possible to continuously improve the technology and manufacturing techniques used in
our products.
This document is protected under copyright
laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy, microfiche, web cam or other methods –
including any electronic systems and media –
requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: +49 (0)62 24 143-0
Fax: +49 (0)62 24 143-268
eMail: histo_info@leica-microsystems.com
Internet: http://www.leica-microsystems.com
Leica CM1510 S – Cryostat
3
Table of contents
1. Important information ................................................................................................................ 6
1.1 Symbols used in the text and their meaning .......................................................................................... 6
1.2 Instrument type ............................................................................................................................................. 6
1.3 Designated use ............................................................................................................................................. 6
1.4 Qualification of personnel .......................................................................................................................... 6
2. Safety ............................................................................................................................................ 7
2.1 Safety instructions ....................................................................................................................................... 7
2.2 Dangers, warnings and cautions .............................................................................................................. 7
2.3 Safety features............................................................................................................................................11
2.3.1 Locking the handwheel ............................................................................................................................. 11
2.3.2 Knife guard ...................................................................................................................................................11
3. Instrument components and specifications ........................................................................ 12
3.1 Overall view - instrument parts ................................................................................................................12
3.2 Technical data ............................................................................................................................................ 14
4. Setup ........................................................................................................................................... 16
4.1 Installation site requirements .................................................................................................................. 16
4.2 Standard delivery - packing list ............................................................................................................... 17
4.3 Unpacking and installation ...................................................................................................................... 18
4.4 Fastening the handwheel ......................................................................................................................... 19
4.5 Electrical connection ................................................................................................................................20
4.6 Prior to operation ........................................................................................................................................20
5. Operation .................................................................................................................................... 22
5.1 Control panel ............................................................................................................................................... 22
5.2 Controller ...................................................................................................................................................... 23
5.3 Setting user-definable parameters.........................................................................................................24
6. Daily work with the instrument ............................................................................................. 26
6.1 Specimen freezing ...................................................................................................................................... 26
6.1.1 Quick-freeze shelf.......................................................................................................................................26
6.2 Inserting the specimen discs .................................................................................................................. 26
6.2.1 Fixing the specimen discs in the specimen head ............................................................................... 27
6.2.2 Specimen orientation ................................................................................................................................ 27
6.3 Inserting the knife or blade into the knife holder ............................................................................... 27
6.4 Sectioning / trimming ................................................................................................................................. 28
6.4.1 Moving the specimen with the coarse / precision feed ....................................................................29
6.4.2 Section thickness setting ......................................................................................................................... 29
6.5 Defrosting ..................................................................................................................................................... 30
6.5.1 Automatic defrosting of the cryochamber ............................................................................................ 30
6.5.2 Manual defrosting of the cryochamber ................................................................................................ 30
6.5.3 Manual defrosting of the quick-freeze shelf ....................................................................................... 31
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Instruction manual V1.4 – 04/2010
Table of contents
6.6 Finishing work..............................................................................................................................................31
6.6.1 Finishing the daily work .............................................................................................................................31
6.6.2 Shutdown for a longer period ..................................................................................................................32
6.7 Temperature selection chart ................................................................................................................... 33
7. Troubleshooting ....................................................................................................................... 34
7.1 Possible causes and remedies ............................................................................................................ 34
7.2 Display error messages ......................................................................................................................... 38
7.3 Thermal circuit breaker (120V model only)....................................................................................... 3 8
8. Cleaning and maintenance .................................................................................................... 39
8.1 General maintenance instructions ..................................................................................................... 39
8.2 Automatic circuit breaker...................................................................................................................... 40
8.3 Cleaning ...................................................................................................................................................... 4 1
8.3.1 Spray disinfection with Leica Cryofect.............................................................................................. 4 1
8.4 Removing the microtome........................................................................................................................ 4 3
8.5 Removing the microtome housing ....................................................................................................... 4 4
8.6 Reinstalling the microtome .................................................................................................................... 45
8.7 Replacing the fuses ................................................................................................................................. 46
8.8 Replacing the lamp .................................................................................................................................. 47
9. Optional accessories ..............................................................................................................48
9.1.1 Mobile heat extractor ............................................................................................................................. 4 8
9.1.2 Stationary heat extractor ...................................................................................................................... 48
9.1.3 Thermal block............................................................................................................................................ 4 9
10. Warranty and service.............................................................................................................. 53
11. Decontamination Certificate (master) ...................................................................................54
12. Information for the People´s Republik of China ................................................................ 56
Leica CM1510 S – Cryostat
5
1. Important information
1.1 Symbols used in the text and their meaning
Dangers, warnings and cautions appear in a gray box and are marked by
a warning triangle .
Notes, i.e. important user information appear in a gray box and are
marked by an information symbol
.
(5)
Figures in brackets refer to item nos.
in drawings.
1.2 Instrument type
All information provided in this manual applies
only to the instrument type indicated on the title
page.
1.3 Designated use
The Leica CM1510S is a powerful cryostat for
routine and research applications in biology,
medicine and industry, where it serves for
quick-freezing and subsequent sectioning of
specimen material.
The instrument is not designed for unattended
storage of specimen material.
The instrument must be operated only according to its designated use (see description
above) and according to the instructions contained in this manual.
Any other use of the instrument will be considered improper.
1.4 Qualification of personnel
• Der Leica CM1510 S may be operated only
by trained laboratory personnel.
• All laboratory personnel designated to operate the Leica CM1510 S must read this instruction manual carefully and must be familiar with all technical features of the
instrument before attempting to operate the
Leica CM1510 S.
A name plate indicating the serial number of
the instrument is attached to the back of the
instrument.
Fig. 1
6
Instruction manual V 1.4 – 04/2010
Be sure to comply with the safety instructions and warnings in this chapter. Be sure to read
these instructions, even if you are already familiar with the operation and use of other
Leica products.
2.1 Safety instructions
2. Safety
This instruction manual contains important instructions and information regarding the operational safety and maintenance of the instrument.
The instruction manual is an important part of
the product, which must be read carefully prior to setup and use and must always be kept
near the instrument.
If additional requirements on accident prevention and environmental
protection exist in the country of operation, this instruction manual must
be supplemented by appropriate instructions to ensure compliance
with such requirements.
This instrument was built and tested in accordance with the safety regulations for electrical
measuring, control, regulating and laboratory
devices.
For current information about applicable standards, please refer to the CE declaration of
conformity on our Internet site:
www.leica-microsystems.com
In order to maintain this condition and ensure
safe operation, the operator must observe all
the instructions and warnings contained in
this instruction manual.
2.2 Dangers, warnings and cautions
The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention. Primarily responsible for accident-free operation is above all
the institution which owns the instrument and, in addition, the designated personnel who
operates, services or repairs the instrument. To ensure trouble-free operation of the instrument, be sure to comply with the following instructions and warnings.
The protective devices on both instrument and accessories must neither be removed nor
modified.
Only authorized and qualified service personnel may repair the instrument and access the
instrument’s internal components.
Leica CM1510 S – Cryostat
7
2. Safety
Warnings - Transport and installation
The instrument must be transported either in an upright position or, if inclined, at an angle
of max. 30 degrees!
Do not operate in rooms with explosion hazard!
To ensure the instrument works trouble-free, the instrument must be set up with a least 10
cm distance from all walls and furniture.
Do not place anything next to the compressor ventilation grid (right side of the cabinet) to
ensure adequate ventilation!
The protective foam parts between microtome and compressor MUST be removed prior to
operating the instrument!
Warnings - Electrical connection
Please refer to and comply with the ‘Technical data‘!
The instrument must be connected to a grounded mains power outlet socket!
During the start-up of the compressor the nominal voltage must not drop below the values
specified in the ‘Technical Data‘ chapter!
The compressor requires a start-up current between 45 and 50 A.
Therefore, the electric circuit at the installation site must be inspected by a qualified electrician to ensure that it meets the requirements for a smooth operation of the instrument.
For trouble-free operation, a constant power supply according to specification must be ensured at all times. Failure to comply with the above will cause damage to the instrument.
After transporting, wait at least 4 hours before switching the instrument on!
This waiting period is necessary to allow the compressor oil, which may have been displaced during transport, to return to its original position.
Failure to comply with this can cause severe damage to the instrument.
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Instruction manual V1.4 – 04/2010
Warnings - sectioning
Be careful when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause severe injury!
Never leave knives/blades or knife holders with a knife/blade mounted lying around!
Always store knives in the knife box when not in use!
Never place a knife anywhere with the cutting edge facing upwards!
NEVER try to catch a falling knife!
Always clamp the specimen before the knife!
Be careful when picking up the sections! The cutting edge is exposed!
When working inside the cryochamber, keep hands away from gilled evaporator – high risk
of injury from sharp edges!
Prior to manipulating knife and specimen, when changing the specimen or during breaks,
always lock the handwheel and cover the cutting edge with the knife guard!
2. Safety
Avoid skin contact with cold parts of the instrument as this can cause frostbite!
Cleaning and disinfection
Do not use organic solvents or any other aggressive substances for cleaning and disinfection! The instrument is suitable for spray disinfection with Leica Cryofect!
Only use alcohol or commercial disinfectants based on alcohol for disinfection!
For spray disinfection follow the instructions for use supplied with the disinfectant!
Do not use any external heaters for drying the cryochamber. This can cause severe damage
to the heating system!
Leica CM1510 S – Cryostat
9
2. Safety
Warnings - maintenance
Only qualified and authorized service personnel may access the internal components of the
instrument for service and repair.
Clean the compressor ventilation grid at least once a year or more often if required!
Replacing the fuses:
Before replacing the fuses, turn off the instrument mains switch and pull the mains plug!
Only use replacement fuses of the same specification. For the required values, please refer
to chapter 3.2 ‘Technical Data‘!
Replacing the fluorescent light lamp:
Before replacing the lamp, turn off the instrument mains switch and pull the mains plug!
If the lamp is broken, it must be replaced by the technical service, as the replacement procedure involves a high risk of injury!
Do not use any lamp type other than the one inserted by the manufacturer!
Removing the microtome from the cryochamber
Prior to removing the microtome turn off the instrument mains switch and pull the mains
plug.
Before removing the microtome, rotate the handwheel until the specimen cylinder is positioned in the lowest position. Otherwise, when removing the microtome, the specimen head
will rapidly fall down and might injure the operator’s hands!
Wear appropriate protective gloves to remove the cold microtome from the cryochamber.
Prolonged skin contact with cold parts of the instrument can cause frost bite!
The microtome must be completely dry before reinstallation. Humidity inside the microtome
will condense and freeze in the cold cryostat and thus may cause malfunctions or damage!
10
Instruction manual V 1.4 – 04/2010
2.3 Safety features
The instrument is equipped with the following safety features: handwheel locking mechanism and knife guard on knife holder.
The consistent use of these safety features and strict observation of
the safety instructions and warnings in this manual will safeguard the
operator from accidents and/or personal injury to a great extent.
2.3.1 Locking the handwheel
2
1
To lock the handwheel, rotate the handwheel until the handle is in the
upper position and the metal pin (1) is located opposite the black dot (2).
Push the metal pin outward into the recess at the handwheel; if necessary move the handwheel slightly back and forth, until you feel that the
locking mechanism engages.
To unlock the handwheel, push the metal pin (1) out of the recess at the
handwheel, towards the cryostat housing.
2. Safety
2.3.2 Knife guard
Leica CM1510 S – Cryostat
Prior to manipulating knife and specimen, when changing the
specimen or during breaks, always lock the handwheel and
cover the cutting edge with the knife guard!
Fig. 2
Knife holder CN is equipped with a knife guard; on the knifeholder CE, the
glass plate of the anti-roll guide constitutes the knife guard (see separate instruction manual for your knife holder).
11
3. Instrument components and specifications
3.1 Overall view - instrument parts
Specimen
disc
Section
waste tray
Brush shelf
Fig. 3
12
Knife holder CN
Knife holder CE
Knife holder base
Instruction manual V 1.4 – 04/2010
3. Instrument components and specifications
Motorized coarse feed
forward or
backward
- slow
Use the motorized coarse feed to rapidly move
the specimen head towards or away from the
knife.
Carefully observe the specimen
when moving it towards the cutting
edge to avoid an accidental collision of the specimen with the knife
edge, which can cause severe damage to both knife and specimen.
forward
or backward
- fast
Display indicating:
- Set / actual temperature
- Real time
- Defrost time
Fig. 4
Control
panel
On/Off switch
for cryochamber
illumination
Heat extractor
Thermal
block
(optional
accessory)
Leica CM1510 S – Cryostat
Quick-freezing shelf
13
3. Instrument components and specifications
3.2 Technical data
Operating temperature range from +18 °C to +35 °C. All specifications related to temperature
are based on an ambient temperature of +22 °C and maximum air humidity of 60%!
Type CM1510S CM1510 S CM1510S
Nominal voltage 100 V AC ±10% 120 V AC ±10% 230 V AC ±10%
Nominal frequency 50/60 Hz 60 Hz 50 Hz
Power draw 2000 VA 1300 VA 1500 VA
Protective class I I I
Mains fuses Automatic circuit Automatic circuit Automatic circuit
breaker T 20 A breaker T 15 A breaker T 10 A
Pollution degree 2 2 2
Overvoltage inst. category II II II
Power draw 495 W / 850 W 850 W
Refrigerating output
at evaporator temperature 470 W / 780 W 780 W
of -25 °C
Nominal pressure 25 bar (maximum value)
Safety factor 3
Refrigerant 215 g ± 5 g R404A *
Compressor oil 0.6 l EMKARATE RL244b, ICI *
Refrigeration system 50 Hz 60 Hz
Temperature setting range 0 °C to -30 °C ±10%
at an ambient temperature of 22°C
_<
and air humidity
60 %
when sliding window is closed;
adjustable in 1-degree increments
Temperature of quick-freezing shelf Max. -44 °C
- at a temperature of -30 °C
Defrosting Automatic hot gas defrosting,
defrost time freely programmable,
manual defrosting of quick-freeze shelf and evaporator
duration: 8 min.
* Refrigerant and compressor oil must be replaced by authorized service personnel only!
495 W
470 W
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Instruction manual V 1.4 – 04/2010
3. Instrument components and specifications
Microtome
Rotary microtome
Section thickness setting 1 -60 µm; in 1-µm steps from 1 to 10 µm
in 2-µm steps from 10 to 20 µm
in 5-µm steps from 20 to 60 µm
Total horizontal specimen feed 25 mm
Vertical stroke 59 mm
Maximum specimen size 55 x 55 mm
Specimen orientation 8° (x-, y-, z-axis)
Electrical coarse feed
slow 0.2 µm/s
fast 0.7 µm/s
Cryocabinet
Dimensions
Width (w/o handwheel) 880 mm
Width (with handwheel) 680 mm
Depth 680 mm
Height (arm rests) 1010 mm
Height (overall) 1175 mm
Please refer to chapter 4.1 - ‘Installation site requirements’!
Weight (incl. microtome) approx. 135 kg
Lamp
50 Hz version: Osram Dulux S 11 W/21, light color LUMILUX bright white
60 Hz version: Osram Dulux S 13 W/21, light color LUMILUX bright white
All CM1510S instrument types require the following secondary fuses:
F4: T0.5 A 6.3 x 32 mm; Fa. Schurter, Typ FST; Fa. Littlefuse, Typ 313
F5: T1.0 A 6.3 x 32 mm; Fa. Schurter, Typ FST; Fa. Littlefuse, Typ 313
F6: T2.0 A 6.3 x 32 mm; Fa. Schurter, Typ FST; Fa. Littlefuse, Typ 313
F7: T4.0 A 6.3 x 32 mm; Fa. Schurter, Typ FST; Fa. Littlefuse, Typ 313
F8: T2.0 A 6.3 x 32 mm; Fa. Schurter, Typ FST; Fa. Littlefuse, Typ 313
Leica CM1510 S – Cryostat
15
4. Setup
4.1 Installation site requirements
Do not operate in rooms with explosion hazard!
To ensure the instrument works trouble-free, the instrument must be set up with at least 10
cm distance from all walls and furniture.
Do not place anything next to the compressor ventilation grid (right side of the cabinet) to
ensure adequate ventilation!
The place of installation must meet the following requirements:
• no direct sunlight,
• mains power socket at a distance no greater than 2.5 m (length of
power cord = 2.5 m) - no extension cords must be used!
• no drafts (air condition outlets etc.),
• even floor,
• virtually vibration-free floor,
• handwheel freely and easily accessible,
• room temperature maximum 35 °C, (< 22 °C recommended),
• relative humidity max. 60 %,
• only for indoor use.
16
High room temperature and excessive air humidity at the installation site affect the cooling
capacity of the cryostat.
Instruction manual V1.4 – 04/2010
4.2 Standard delivery - packing list
1 Basic instrument .................................................................... 14 0436 38461
1 Stationary heat extractor ..................................................... 14 0369 11197
1 Handwheel with marking, antibacterial ........................... 14 0471 42558
1 Optional clamping lever for object head .......................... 14 0112 28806
1 Set of specimen discs ........................................................... 14 0470 43550
-4 Specimen discs, 25 mm .................................................... 14 0416 19275
-4 Specimen discs, 30 mm .................................................... 14 0370 08587
1 Adapter for Miles Tissue Tek specimen discs ................ 14 0436 26747
1 Section waste tray ................................................................. 14 0471 30787
1 Storage shelf, right ................................................................. 14 0471 30789
1 Storage shelf, left ................................................................... 14 0436 38503
1 Brush shelf............................................................................... 14 0398 13088
1 Tool set: .................................................................................... 14 0436 43463
-1 Brush, fine ........................................................................... 14 0183 28642
-1 “Leica” brush w/magnet ................................................. 14 0183 40426
-1 Allen key, size 1.5 ............................................................... 14 0222 10050
-1 Allen key, size 2.5 ............................................................... 14 0222 04137
-1 Allen key, size 3.0 ............................................................... 14 0222 04138
-1 Allen key, size 4.0 ............................................................... 14 0222 04139
-1 Allen key with spherical head, size 4.0 ......................... 14 0222 32131
-1 Allen key, size 5.0 ............................................................... 14 0222 04140
-1 Allen key with handle, size 5.0 ........................................ 14 0194 04760
-1 Allen key, size 6.0 ............................................................... 14 0222 04141
-1 Single-head wrench, size 13 .......................................... 14 0330 33149
-1 Single-head wrench, size 16 .......................................... 14 0330 18595
1 Bottle of cryostat oil, type 407, 50 ml .................................. 14 0336 06098
1 Bottle of OCT-Compound, mounting medium
for cryosectioning, 125 ml .................................................... 14 0201 08926
1 Instruction manual Leica CM1510 S - G/E/F/S ................. 14 0436 80001
4. Setup
Order no.
Leica CM1510 S – Cryostat
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