Leica CM1100 User Manual
Leica CM1100
Cryostat
Instructions for use
Leica CM1100 V1.3 RevB English – 09/2012
Order-No.: 14 0469 80101, RevB
Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
1. Important information
Serial No. .....................................................................
Year of manufacture: .................................................
Manufactured: ........ Federal Republic of Germany
Until the ratification of the guideline for in-vitro diagnostic instruments this product is categorized
according to the MedGV (National Regulations for
Medical Appliances) as a Class 3 equipment.
The information, numerical data, notes and value judgments contained in this manual represent the current
state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional
copies, updates etc. of this manual.
For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability as far as permissible according to the national
legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or
related to compliance with statements or other information in this manual.
Statements, drawings, illustrations and other information as regards contents or technical details of the
present manual are not to be considered as warranted
characteristics of our products. These are determined
only by the contract provisions agreed between ourselves and our customers.
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17-19
D-69226 Nussloch
Germany
Telephone: 062 24 /1 43-0
Telefax: 0 62 24/143-2 68
web: http://www.LeicaBiosystems.com
Leica reserves the right to change technical specifications as well as manufacturing processes without
prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products.
This document is protected under copyright laws. Any
copyrights of this document are retained by Leica
Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any
parts thereof) by means of print, photocopy, microfiche,
web cam or other methods – including any electronic
systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the name plate at the back of
the instrument.
© Leica Biosystems Nussloch GmbH
Leica CM1100 – Cryostat
3
2. Table of contents
1. Important information ....................................................................................................................................................3
2. Table of contents ............................................................................................................................................................ 4
3. Safety instructions for handling the instrument........................................................................................................5
3.1 Safety features ............................................................................................................................................................................................ 5
3.1.1 Locking the handwheel .............................................................................................................................................................................. 5
3.1.2 Knife guard ................................................................................................................................................................................................... 5
3.2 General hazards .......................................................................................................................................................................................... 5
4. Technical data ................................................................................................................................................................ 8
5. General description .......................................................................................................................................................9
5.1 Leica CM 1100 Overview ............................................................................................................................................................................ 9
5.2 Product description .................................................................................................................................................................................. 10
5.3 Standard delivery range........................................................................................................................................................................... 10
6. Unpacking and installation .................................................................................................................................... 11
6.1 Unpacking................................................................................................................................................................................................... 11
6.2 Site requirements ..................................................................................................................................................................................... 12
6.3 Transport to the installation site ............................................................................................................................................................. 12
7. First use of the instrument ....................................................................................................................................... 13
7.1 Connection to mains power .................................................................................................................................................................... 13
7.2 Installing the accessories........................................................................................................................................................................ 13
7.3 Switching on .............................................................................................................................................................................................. 14
7.4 Functions of the control unit ................................................................................................................................................................... 14
7.4.1 LEDs ............................................................................................................................................................................................................. 14
7.4.2 Function keys ............................................................................................................................................................................................. 14
7.4.3 Setting user definable parameters ........................................................................................................................................................ 15
7.4.4 Starting and terminating a manual defrost cycle ............................................................................................................................... 15
7.5 Temperature selection chart (in minus °C) .......................................................................................................................................... 16
8. Daily operation ........................................................................................................................................................... 17
8.1 Specimen discs ......................................................................................................................................................................................... 17
8.1.1 Specimen freezing.................................................................................................................................................................................... 17
8.1.2 Fixing the specimen disc in the specimen head ................................................................................................................................. 17
8.2 Knife holder CE .......................................................................................................................................................................................... 18
8.2.1 Changing the rear pressure plate .......................................................................................................................................................... 18
8.2.2 Adjusting the rear pressure plate .......................................................................................................................................................... 18
8.2.3 Adjusting the front pressure plate .......................................................................................................................................................... 19
8.2.4 Inserting the blade .................................................................................................................................................................................... 19
8.2.5 Lateral displacement ................................................................................................................................................................................ 20
8.2.6 Adjusting the anti-roll plate..................................................................................................................................................................... 20
8.2.7 Replacing the glass anti-roll plate......................................................................................................................................................... 21
8.3 Coarse feed wheel.................................................................................................................................................................................... 22
8.4 Trimming .................................................................................................................................................................................................... 22
8.5 Sectioning .................................................................................................................................................................................................. 23
8.6 Section transfer ......................................................................................................................................................................................... 23
8.7 Defrosting ................................................................................................................................................................................................... 24
8.7.1 Programming an automatic defrost cycle ............................................................................................................................................ 24
8.7.2 Starting a manual defrost cycle ............................................................................................................................................................. 24
9. Trouble sh ooting ....................................................................................................................................................... 25
10. Cleaning and disinfection ....................................................................................................................................... 28
10.1 Cleaning...................................................................................................................................................................................................... 28
10.2 Spray disinfection with Leica Cryofect ................................................................................................................................................. 28
10.3 Removal of the microtome ...................................................................................................................................................................... 29
10.4 Reassembly of the microtome ................................................................................................................................................................ 30
11. Maintenance............................................................................................................................................................... 31
11.1 General maintenance............................................................................................................................................................................... 31
11.2 Fuse replacement ..................................................................................................................................................................................... 31
12. Optional accessories ............................................................................................................................................... 32
12.1 Mobile heat extractor............................................................................................................................................................................... 32
12.2 Thermal block............................................................................................................................................................................................ 32
13. Warranty and service ............................................................................................................................................... 33
14. Decontamination Certificate
16. Information for the People´s Republic of China ................................................................................................ 35
(master) ...................................................................................................................... 34
4 Instructions for use V1.3 RevB – 09/2012
3. Safety instructions for handling the instrument
3.1 Safety features
The instrument design incorporates the following
safety features: handwheel lock, glass anti-roll guide
of the knife holder CE that has the protective function
of a knife guard.
3.1.1 Locking the handwheel
Prior to manipulating the knife holder and
specimen, or changing the specimen or knife,
and during breaks, always lock the
handwheel!
The handwheel can be locked in the upper turning
point.
2
1
3.1.2 Knife guard
Prior to manipulating the knife holder and
specimen, or changing the specimen or knife,
and during breaks, always cover the cutting
edge with the anti-roll guide!
The knife holder CE for disposable blades has a glass
anti-roll guide which is positioned over the knife edge
while sectioning to prevent injury thus acting as a knife
guard.
3.2 General hazards
Despite the safety features provided for operator
safety, the use of the instrument and the applications
for which is designed involve certain risks that cannot
be totally eliminated:
• The handling of disposable blades involves a considerable risk of injury.
Fig. 5.1
• Rotate the handwheel until the handle (1) is positioned in the upper turning point.
• To lock, push the lever (2) towards the housing.
• To unlock, pull the lever (2) away from the housing.
• Extended skin contact with cold parts of the instrument can cause frostbite!
• Sectioning infectious and/or radioactive materials
constitutes a hazard.
Leica CM1100 – Cryostat
5
3. Safety instructions for handling the instrument
Transport and installation
• The instrument must be transported in an
upright position only, or at an angle of max.
30 °!
• Do not carry or reposition the instrument
by holding it at parts of the housing or at
the handwheels! Only use the carrying
handles at the front and rear for carrying!
• Prior to transporting the instrument, pull
out the retractable handles of the handwheel and coarse feed wheel and place
them in the depression provided in the
center of the wheel!
• Do not operate in rooms with explosion
hazard!
• To ensure an adequate cooling capacity,
the instrument must be set up with at least
10 cm distance from walls and furniture!
• Do not place anything next to the compressor ventilation grids to ensure adequate
ventilation at all times!
Connection to mains power
• Please refer to the 'Technical data'!
• The instrument must be connected to a
grounded mains power outlet socket.
• During the start-up of the compressor the
nominal voltage must not drop below the
values specified in the 'Technical data'.
Please note that the compressor requires
a start-up current between 10 and 25 A.
Therefore, the electric circuit at the instal-
lation site must be inspected by an electrical engineer to ensure that it meets the
requirements for a smooth operation of the
instrument.
A constant adequate power supply to the
instrument must be ensured at all times.
Failure to comply with the above will
cause severe damage to the instrument.
• Caution:
240 V units may be operated only with the
step-up transformer supplied with the instrument!
Failure to comply with this will cause se-
vere damage to the instrument!
• After transporting, wait at least 4 hours before turning the instrument on.
This waiting period is necessary to allow
the compressor oil, which may have been
displaced during transport, to return to its
original position.
Failure to comply with this can cause severe damage to the instrument.
6 Instructions for use V1.3 RevB – 09/2012
3. Safety instructions for handling the instrument
Sectioning
• Take care when handling disposable
blades.
The cutting edge is extremely sharp and
can cause severe injury!
• Never leave the knife holder with a blade
mounted lying around!
• Dispose of used blades by inserting them
into the receptacle provided at the bottom
of the disposable blade dispenser!
• Always clamp the specimen before the
blade!
• Take care when removing the section - the
cutting edge is exposed!
• Prior to manipulating the knife holder and
specimen, or changing the specimen or
blade, and during breaks, always lock the
handwheel and cover the cutting edge
with the anti-roll guide!
Removal of the microtome
• Before removing the microtome, turn the
instrument off with the mains switch!
• Wear appropriate protective gloves to remove the cold microtome from the cryochamber! Risk of frost bite!
Or wait until the microtome has reached
room temperature.
• The microtome must be completely dry before reinstallation. Humidity inside will
condense and freeze in the cold cryostat
and thus may cause malfunctions or damage.
Maintenance
• Only qualified and authorized service personnel may access the internal components of the instrument for service and repair.
• Avoid extended skin contact with cold
parts of the instrument as this can cause
frostbite!
Cleaning and disinfection
• Do not use organic solvents or any other
aggressive substances for cleaning and
disinfection!
We strongly recommend the use of Leica
Cryofect disinfectant spray!
Only use alcohol or common disinfectants
based on alcohol!
• For spray disinfection follow the instructions for use supplied with the disinfectant!
• Do not use external heaters for drying the
cryochamber. This can cause damage to
the slot cover!
Fuse replacement
• Only use a fuse of the same specification!
For the required value, please refer to
Chapter 4. 'Technical data' or to the fuse
specification label at the rear of the instrument.
Leica CM1100 – Cryostat
7
4. Technical data
Operating temperature range: 18 °C to 35 °C.
All specifications related to temperature are valid only for an ambient temperature up to 22 °C and for an air
humidity lower than 60%!
Type CM 1100-1 CM 1100-3 CM 1100-11
Nominal voltage 230 V AC ±10% 120 V AC ±10% 100 V AC ±10%
Nominal frequency 50 Hz 60 Hz 50/60 Hz
Power draw
during refrigeration 170 VA 170 VA 170 VA
during defrosting for 10 min. 320 VA 400 VA 650 VA
Maximum start-up current for 5 sec. 10 A
Protective class I I I
eff.
25 A
eff.
Mains fuse 2-pole, T 10 A 2-pole, T 10 A 2-pole, T 10 A
Pollution degree 2 2 2
Overvoltage installation category II II II
Heat output
during refrigeration 170 J/s 170 J/s 170 J/s
during defrosting for 10 min. 320 J/s 400 J/s 650 J/s
Transformer fuse protection F3 T 4 A T 4 A T 4 A
acc. to DIN-IEC 127-II UL-listed UL-listed
25 A
eff.
Refrigeration
Temperature range 0 °C to -30 °C ±10%
at an ambient temperature of 22 °C
_<
and air humidity
60% when lid is closed;
adjustable in 1 K increments
Defrosting automatic hot gas defrosting,
defrost time freely programmable; 1 defrost cycle/ 24 h,
manual defrosting
duration: 20 min.
Power draw 226 W 204 W 235 W /269 W
Refrigerating output
1)
193 W 185 W 190 W
Maximum nominal pressure 15 bar 15 bar 15 bar
Safety factor 3 3 3
Refrigerant 170 g ± 5 g R134a * 170 g ± 5 g R134a * 155 g ± 5 g R134a *
Compressor oil 0.4 l alpha 22, Kyodo * 0.4 l alpha 22, Kyodo * 0.4 l alpha 22, Kyodo
* Refrigerant and compressor oil must be replaced by authorized service personnel!
1)
Rating acc. to ASHRAE: Evaporating temperature -23.3 °C
Liquefier temperature 54.4 °C
Ambient temperature 32.2 °C
Microtome
*
Type Rotary microtome
Section thickness setting range 0 - 20 μm, continuously adjustable
Division of the scale 2 μm
Horizontal specimen feed 15 mm ± 1 mm
Vertical stroke 46 mm ± 1 mm
Maximum specimen size 36 mm
Cryocabinet
Dimensions (W x D x H) 570 x 780 x 380 mm
Weight (including microtome) 50 kg
8 Instructions for use V1.3 RevB – 09/2012
5.1 Leica CM 1100 Overview
Cryochamber
lid
Carrying
handles
5. General description
Control unit
Handwheel
with retractable
countersunk handle
Fig. 9.1
Compressor
ventilation
grids
Coarse feed
wheel with
retractable
countersunk
handle
Lever
for locking the handwheel
Mains switch
Cryochamber
Fig. 9.2
Leica CM1100 – Cryostat
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5. General description
5.2 Product description
The Leica CM1100 is a portable compact cryostat for rapid freezing and manual sectioning of tissue specimens.
Two carrying handles on the front and rear of the housing make it easy for two persons
to transport the instrument. The counterbalanced handwheel is lockable in the top position of the handle.
The corrosion-proof cryochamber is easy to clean and easily accessible after removing the lid. The cryochamber temperature is selectable between 0°C and -30 °C.
The stainless steel rotary microtome is virtually maintenance-free in operation. It is designed for sections in a range of 3μm or 4 μm up to 20μm.
All push button controls and the display are integrated in the control unit.
Important parameters such as set temperature and defrost time can be programmed.
Cryochamber defrosting takes 20 minutes. It is possible to program an automatic de-
frost cycle for a certain time of the day and to activate a manual defrost cycle when required.
5.3 Standard delivery range
Standard delivery includes:
1 Basic instrument
1 Accessory-kit consisting off:
1 Brush, fine .....................................................................................................................................................14 0183 28642
1 Leica-brush ...................................................................................................................................................14 0183 30751
1 Bottle of OCT-compound, mounting medium for cryosectioning, 125ml ............................................ 14 0201 08926
1 Allen key, size 4 ............................................................................................................................................14 0222 04139
1 Bottle of cryostat oil, type 407, 50 ml ......................................................................................................... 14 0336 06098
1 Replacement glass anti roll guide for CE-holder, 70mm wide ..............................................................14 0419 33813
2 Specimen discs, 20mm ............................................................................................................................... 14 0370 08636
1 Section waste tray, large............................................................................................................................ 14 0469 31779
1 Section waste tray, smal ............................................................................................................................ 14 0469 31780
1 Freezing shelf ............................................................................................................................................... 14 0469 31782
1 Knife holder CE for low profile blades ...................................................................................................... 14 0504 33996
1 Pressure plate for high profile blades, 22° ..............................................................................................14 0504 32199
2 Fuses 5x 20 T 4.0A........................................................................................................................................ 14 6943 04000
1 Pair of cut resistant gloves, size S ............................................................................................................ 14 0340 40859
1 Instructions for use "Leica CM1100" -G/E/F/S .................................................................................... 14 0469 80101
10 Instructions for use V1.3 RevB – 09/2012
6. Unpacking and installation
6.1 Unpacking
3
Check the transport crate for external damage on arrival of the shipment.
If damages are obvious, please make a complaint to your carrier.
• Remove 2 screws (1) at the top and bottom on all of
2
the four sides of the crate (2).
• Remove the lid (3).
• Lift the body of the crate (2) straight up.
1
1
1
1
• Take the accessory box (4) from the pallet (5).
• Remove the foam plate (6).
• Remove the inner cardboard box (7) by lifting it upwards.
6
• Unscrew the 4 angle brackets (8) by loosening 2
screws each.
Do not carry or reposition the instrument by
holding it at parts of the housing or at the
handwheels!
Only use the carrying handles at the front and
rear for carrying!
7
• Hold the instrument at the carrying handles (9) at
the front and rear and lift it from the pallet.
10
4
Fig. 11
• Take the insert (10) out of the accessory box (4) and
remove all accessories.
9
8
8
5
Leica CM1100 – Cryostat
11
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