Jenway 6715, 6705, 6700 Instruction Manual

67 Series Spectrophotometers Models 6700, 6705 & 6715

Operating Manual

670 005/REV D/11-11

1

Contents

Page Safety 7

Guarantee 8

SECTION 1 - Introduction

1.1 Instrument Description 9

1.2 Instrument Speciﬁcations 10-11

1.3 Good Practice Guidelines 11-12

SECTION 2 - Getting Started

2.1 Unpacking 13-14

2.2 Installation 14-15

2.2.1 Location 14

2.2.2 Supply voltage 14

2.2.3 Mains connections 14

2.2.4 Touch screen functions 15

2.2.5 Toolbar icons 16-20

2.2.6 Rear panel layout 21

2.2.7 Power on and self tests 22

SECTION 3 - Systems of Operation

3.1 Multi-User and Free Operation 23

3.2 Memory Structure 23

3.2.1 Memory selection 23

3.2.2 Memory Hierarchy 24

3.3 Creating Methods 24-25

3.4 File and Data Management 25-26

SECTION 4 - Instrument Settings

4.1 Menu Options 27

4.2 Set Time & Date 27

4.3 User Preferences 27

4.3.1 Adjust User PIN 28

4.3.2 Language 28

4.3.3 Method View Settings 28

4.3.4 Touch Screen Click 29

4.3.5 Brightness 29

4.3.6 Adjust Date & Time 29

4.4 About 30

4.5 Form Feed 30

4.6 Administration Functions 30

4.6.1 Instrument ID 31

4.6.2 Screen Dump 31

4.6.3 Modify Users 31

4.6.3.1 Create New User 32

4.6.3.2 Delete User 32

4.6.3.3 Delete All Users 32

4.6.3.4 Reset User PIN 33

4.7 Memory Manager 33

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SECTION 5 - Photometrics Mode

5.1 Principles of Measurement 34

5.2 Free Operation 34-37

Setting Method ID 35 Setting Wavelength 36 Setting Method Security 36 Setting Batch ID 36 Auto Log Settings… 37

5.3 Logged In Measurement 38-41

Creating a New Method 39 Setting Method ID 39 Setting Wavelength 39 Setting Method Security 40 Setting Batch ID 40 Auto Log Settings… 41

File & Data Management 42-43

5.4 Performing Measurements – all users 44

Zeroing the Instrument 44 Measuring the Sample 44

SECTION 6 - Spectrum Mode

6.1 Principles of Measurement 45

6.2 Free Operation 45-52

Setting Method ID 46 Selecting Measurement Mode 46 Setting Wavelengths (Start & End) 47 Selecting Plot Interval 48 Setting Method Security 49 Setting Additional Set-up Parameters using the Toolbar Icons 49-52 Setting Analysis Points 49 Setting Auto-Scale 50 Selecting Plot Colour 50 Selecting Axis Colour 51 Setting Batch ID 51 Setting Graph Data Points 51 Selecting Printer Media 52

Auto Log Settings… 52

6.3 Logged In Measurement 53-56

Creating a New Method 54 Setting Method ID 54 Selecting Measurement Mode 54 Setting Wavelengths (Start & End) 55 Selecting Plot Interval 56 Setting Method Security 56 Setting Additional Set-up Parameters using the Toolbar Icons 57-60 Setting Analysis Points 57 Setting Auto-Scale 58 Selecting Plot Colour 58 Selecting Axis Colour 59 Setting Batch ID 59

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Setting Graph Data Points 59 Selecting Printer Media 60 Auto Log Settings… 60 File & Data Management 61-62

6.4 Performing Measurements – all users 63-69 Performing a Scan 63 Peforming a Baseline 63 Scanning the Sample 63-64 Analysing the Scan 64-65 Area Under Curve 65 Co-ordinate Tagging 66 Auto Peak & Valley Tagging 66-67 Obtaining Derivative Spectra 67 Overlaying Scans 68 Adding or Subtracting Spectra 69

SECTION 7 - Multi-Wavelength Mode

7.1 Principles of Measurement 70

7.2 Free Operation 70-75

Setting Method ID 71 Selecting Measurement Mode 72 Selecting Sample Style 72 Setting Method Security 72 Setting Wavelengths 73 Calculations 73 Sum 73-74 Setting Batch ID 74 Selecting Printer Media 74 Auto Log Settings... 75

7.3 Logged In Measurement 76-83

Creating a New Method 77 Setting Method ID 77 Selecting Measurement Mode 77 Selecting Sample Style 78 Setting Method Security 78 Setting Wavelengths 79 Calculations 79 Sum 79-80 Setting Batch ID 80 Selecting Printer Media 80 Auto Log Settings… 81 File & Data Management 82-83

7.4 Performing Measurements – all users 84

SECTION 8 - Kinetics Mode

8.1 Principles of Measurement 85

8.2 Free Operation 85-93

Setting Method ID 86 Setting Wavelength 87 Selecting Measurement Mode 87 Run Settings… 87-89

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Setting Run Time 88 Setting Lag Time 88 Setting Start On Level 88 Setting Start Level 89 Setting Method Security 89 Standard 89 Factor 90 Selecting Resolution 90 Selecting Units 90 Setting Auto-Scale 91 Selecting Axis Colour 91 Setting Batch ID 92 Setting Graph Data Points 92 Selecting Printer Media 92 Auto Log Settings… 93

8.3 Logged In Measurement 94-104

Creating a New Method 95 Setting Method ID 95 Setting Wavelength 95 Selecting Measurement Mode 96 Run Settings… 96-97 Setting Run Time 96 Setting Lag Time 97 Setting Start On Level 97 Setting Start Level 97 Setting Method Security 98 Setting Concentration Parameters 98-99 Standard 98 Factor 99 Selecting Resolution 99 Selecting Units 99 Setting Auto-Scale 100 Selecting Axis Colour 100 Setting Batch ID 101 Setting Graph Data Points 101 Selecting Printer Media 101 Auto Log Settings… 102 File & Data Management 103-104

8.4 Performing Measurements – all users 105

SECTION 9 - Quantitation Mode

9.1 Principles of Measurement 106

9.2 Free Operation 106-113

Setting method ID 107 Selecting Measurement Mode 108 Setting Wavelength 108 Selecting Resolution 108 Selecting Units 109 Replicates Setup… 109 Setting Method Security 109 Selecting Calibration Standards 110

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Setting Curve Fit 110 Setting Auto Scale 111 Selecting Plot Colour 111 Selecting Axis Colour 112 Setting Batch ID 112 Setting Graph Data Points 112 Selecting Printer Media 113 Auto Log Settings… 113

9.3 Logged In Measurement 114-123

Creating a New Method 115 Setting Method ID 115 Selecting Measurement Mode 115 Setting Wavelength 116 Selecting Resolution 116 Selecting Units 116 Replicates Setup… 117 Setting Method Security 117 Selecting Calibration Standards 118 Setting Curve Fit 118 Setting Auto Scale 119 Selecting Plot Colour 119 Selecting Axis Colour 120 Setting Batch ID 120 Setting Graph Data Points 120 Selecting Printer Media 121 Auto Log Settings… 121 File & Data Management 122-123

9.4 Performing Measurements – all users 124 Post Quantitation Tools 125

SECTION 10 - Fitting & Using Alternative Modules/Accessories

10.1 Integral Printer Unit 126

10.2 Cell Holder Modules 127

10.3 Automatic 8 & 6 Cell Changer Modules 128-132

10.4 Sipper Pump Module 133-139

10.5 Peltier Module 140-142

10.6 Sipper/Peltier Module 143-151

SECTION 11 - Maintenance & Troubleshooting

11.1 General 152

11.2 Light Source Replacement 152-155

Tungsten lamp – Model 6700 153

Flash lamp module – Models 6705/6715 154-155

SECTION 12 - Declaration of Conformity 156-158

Glossary of

Icons 159-161

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7

Safety

This is important information; please read carefully before installing or using this instrument.

1. The 67 Series Spectrophotometers are designed for operation by trained personnel who are

aware of the principles and applications involved. For further help and advice please contact your local distributor, e-mail sales@jenway.com or visit www.jenway.com

2. The spectrophotometer is a sensitive electronic and optical instrument designed for use in a laboratory environment. Careful adherence to the installation instructions must be observed. If in doubt contact a relevant and competent authority for advice before proceeding.

3. In addition to observing the instructions detailed in the Operating Manual and Service Manual for this instrument all installation, operating and service personnel must be aware of, and employ, a safe

system of work.

4. Voltage levels hazardous to life are present in this instrument, for personal safety only trained engineers aware of the risk and avoidance of electric shock should remove protective covers from the

instrument.

5. This instrument is designed for minimal maintenance, which must be carried out carefully following the procedures detailed in this manual. All safety instructions in these procedures, as well as those deﬁned locally for the area or environment where the work is being carried out must be observed.

6. Other than for those items deﬁned in the maintenance procedures herein there are no user serviceable items in this instrument. Removal of covers and attempted adjustment or service by

unqualiﬁed personnel will invalidate any warranty and incur additional charges for repair.

7. Reference should always be made to the Health and Safety Data for any chemicals or reagents used. All available information, advice and warnings on the handling, storage, use and disposal of such must be carefully observed. When not available this data must be requested from the supplier before proceeding in any way.

8. It is important that good laboratory practice is observed when handling samples, chemicals, reagents and ancillary equipment in order to carry out measurement and analysis with this instrument. Suitable safety and personal protective equipment must be used at all times.

9. If it is suspected that safety protection has been impaired in any way, the spectrophotometer must be made inoperative and secured against any intended operation. The fault condition must be reported to the appropriate servicing authority. In all such reports the model number and serial

number of the spectrophotometer must be quoted.

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Guarantee

Please read this important guarantee information:

Notwithstanding the description and speciﬁcation(s) of the instruments contained in the Operating Manual, Jenway hereby reserves the right to make changes as it sees ﬁt to the instruments or to any components of the instruments.

This Manual has been prepared solely for the convenience of Jenway customers and nothing in this Manual shall be taken as a warranty, condition or representation concerning the description, merchantability, ﬁtness for purpose, or otherwise of the instruments or components.

The 67 Series spectrophotometers are guaranteed for a period of 3 years from the date of purchase.

Within this period we undertake to supply replacements free of charge for parts that may on examination prove to be defective, provided that the defect is not the result of misuse, accident or negligence.

On all correspondence, please quote the model and serial number in full and/or the sales order number.

Any instrument requiring service under this guarantee should be taken to the supplier through whom it was purchased, or, in the case of difﬁculty, it should be carefully packed in its original packaging and consigned, carriage paid, to us. Jenway takes no responsibility for returned goods damaged in transit.

Returned goods will not be processed without a Returns Authorisation Number.

Call the Service Administrator +44 (0) 1785 810475 for the relevant documentation.

Please write the Returns Number on the outside of any packaging and ensure that a copy of a Decontamination Certiﬁcate is visible.

Please register online www.jenway.com or complete and return the Registration Document by fax or mail.

The Guarantee will be rendered invalid if any speciﬁed non-serviceable parts within the instrument are tampered with (i.e. the monochromator).

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SECTION 1 - Introduction

1.1 INSTRUMENT DESCRIPTION

The 67 Series offer a range of unique features and functions to help in the management of methods and data at both individual and multi-user levels.

These units have a specially designed user interface based on a high quality, colour TFT LCD with touch screen technology and QWheel™ support to provide ease-of-use for all operations from set-up to measurement and data handling.

Five main measuring modes are available: Photometrics, Spectrum, Kinetics, Quantitation and

Multi-Wavelength.

The 67 Series have dual memory options that enable you to store settings and data to either internal memory or on a removable SD/Multi-Media memory card. By saving to the removable SD card all settings, methods and results speciﬁc to the user can be retained by the individual, offering additional security and allowing easy transfer of data to a PC for storage, manipulation or off-line review. The SD card can also be used in any similar model, giving complete ﬂexibility to use any available instrument. A simple back-up procedure enables easy transfer of all information from the internal memory to the removable media while quickly enabling a group of instruments to be cloned with identical settings.

It is possible for any user to perform simple measurements (Free Operation) at any time, however, logging-in with your own PIN code will allow you to create methods, change settings or store results. Each method can be designated as Public (for open user access), Read-Only (available for all users as read-only information) or Personal (only accessed through your PIN code).

A number of sampling accessories are available as complete, easily interchangeable modules. These include a Sipper Pump, Sipper/Peltier, Automatic Cell Changer, plus a comprehensive range of passive cuvette and test tube holders, which can be ﬁtted in the single cell holder accessory.

The PC Software supplied enables the transfer of data from the instrument or SD card to the PC with rapid copy and paste into spreadsheets or other computer programmes.

QWheel is a registered trademark of Quantum Research

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1.2 INSTRUMENT SPECIFICATIONS

Technical Speciﬁcation

6700 6705 6715

Light Source Tungsten Halogen Xenon Xenon

Spectral Bandwidth 4nm 4nm 1.5nm

Stray Light <0.1% at 340nm <0.05% at 220nm <0.05% at 220nm

Wavelength Range 320-1100nm 190-1100nm 190-1100nm

Common Speciﬁcations

Optics Sealed, MgF Coated, Split Beam

Wavelength Resolution 0.1nm

Wavelength Accuracy ±1.0nm

Wavelength Repeatability ±0.2nm

Photometric Ranges -0.300 to 3.000A & 0 to 199.9%T

Photometric Resolution 0.001A & 0.1%T

Photometric Accuracy ±0.005 at 1A

Photometric Stability <0.001A per hour

Quantitation Range -99999 to +99999

Number of Standards 20 with up to 5 replicates of each

Curve Fit Algorithms Linear, Quadratic and Cubic Functions

Multi-wavelength Data Points Up to 4 wavelengths

Calculations Difference and ratio

Kinetics Time Limits 30 to 9999 seconds

Kinetics Calibration Standard or factor

Scan Speed 1500nm/minute at 0.1nm data steps

Post Scan Analysis Peak/Valley pick, Peak Ratios, Area, Zoom, Wavelength Table,

Removable Media MM/SD Memory Card or SD/USB memory card

Interface USB, Centronics, Analogue

PC Software Supplied on CD-ROM with USB interface cable

Mains Supply 100 to 230V ac 50 or 60Hz

Sample Compartment 150mm (max. height) x 130mm (w) x 210mm (d)

Size 490x390x220mm

Weight 7.5Kg

Derivatives, Smoothing Conﬁguration Secure Multi-User and Free Access

Number of Users 10 + Supervisor

Number of Methods >500 (on internal ﬂash memory or removable media)

Results Storage >500 (on internal ﬂash memory or removable media)

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Environmental Operating Conditions:

The 67 Series is designed to work safely under the following conditions:

Temperature 15 to 40ºC

Humidity 0 to 90%RH

Accessory Speciﬁcations

Peltier

Temperature Range: 20 to 50° C or 68 to 122° F

Temperature Resolution: 0.1° C or 1° F

Accuracy: ±0.5° C

Stability: ±0.3° C

Red/Green Window: ±0.5° C

Sipper Pump

Modes:

Continuous Aspiration Flow rate dependent on tube ID. (12ml/min with supplied tube)

Timed aspiration sample/air-gap/wash sample volumes 75µl min / 9.5ml max. segment run time 48 secs max.

Memory Non-volatile

1.3 GOOD PRACTICE GUIDELINES

1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free

atmosphere. When in use ambient temperature and light levels should remain as constant as possible.

2. Adherence to Standard Operating Procedures (SOP) and Good Laboratory Practice (GLP) should be monitored with regular calibration checks and a suitable Quality Control (QC) programme.

3. The sample chamber lid must be fully closed during measurement and before any readings are recorded or printed.

4. The correct selection of sample containers is imperative for accurate and reproducible results:

a) Check that the material of the sample container is compatible with the wavelengths to be used

for measurement. In general glass can only be used down to 360nm or 320nm depending on quality. Standard plastic cuvettes can be used down to 320nm. Special UV versions can be used down to 260nm. Below this level quartz cuvettes must be used.

b) Plastic disposable cuvettes should be used ONCE only.

c) Glass cuvettes should be thoroughly cleaned after use. Discard when scratches become evident in

optical surfaces.

d) Care should be taken when selecting semi-micro or micro cuvettes. The cuvette window on the inner chamber (the area ﬁlled with sample) must be wider than the aperture in the sample holder or light will reach the detector without passing through the sample. In this case, semi-micro or micro cuvettes with self-screening black surrounds must be used or alternative holders for these cuvettes ﬁtted.

e) Glass test tubes and other sample tubes should be used with care. Where possible, matched tubes should be used and any index mark set to the correct position before measurements are made.

f) Ensure any sample containers used are compatible with the constituents of both the samples and standards they are to hold. Plastic cuvettes are not compatible with organic solvents.

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g) All sample containers must be handled with care; by the top and non-optical surfaces only. Any

ﬁnger marks evident must be removed using a suitable cleaning process.

h) Flow-through cuvettes must be selected with care and consideration for the sample type, sample volume, pumping system, rinse, sample and waste handling to be used.

5. Samples and standards should not be stored in open cuvettes or sample containers as evaporation will change the value and lead to staining of the walls which may be irreversible. If stored in stoppered and sealed cuvettes, they should be ﬁlled with little or no air space and the values regularly checked against a reference standard or quality control material.

6. Cold samples should be allowed to equilibrate to ambient temperature before measurement (unless a suitable temperature controlled sample holder is in use). Temperature change during measurement may cause air bubbles to form on the walls of the sample holder. This is a common cause of drift during measurement.

7. In the preparation of samples and standards high grade borosilicate glass and AR grade chemicals and reagents must be used. Good quality deionised water or other suitable solvent must be used for dissolving or diluting samples, chemicals and reagents.

8. All measurements require calibration to a blank, for maximum accuracy this should be prepared with care using the same deionised water or solvent used for dissolving or diluting the sample. Where reagents are added to the sample to produce a colour proportional to its concentration a ‘sample based’ blank should be used. In this case the blank should consist of the sample plus any reagents or chemicals to be used, except those that produce the colour to be measured.

9. Deviations from the Beer-Lambert Law may occur at high and low concentrations giving non-linear response during sample concentration measurements. For all new methods a linear range should be deﬁned by the preparation of a calibration curve. The quantitation mode may be used to construct such a curve against which sample results are automatically measured.

10. Cuvettes and sample holders must be ﬁlled to a minimum level which covers the light path.

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SECTION 2 - Getting Started

2.1 UNPACKING INSTRUCTIONS

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Refer to label 1 on carton exterior and ensure instrument type and options/ accessories supplied are correct. Refer to Distributor in the event of any discrepancy.

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Check each item as it is removed from the packaging to ensure it is correct and undamaged. Refer to Distributor if any item is missing or damaged.

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Remove Documentation carton 2. Note that this contains the Instruction Manual and other important documents, which MUST be retained for future reference. Other small items (CD ROM, SD Card etc) will also be found inside this carton.

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Please take some time to familiarise yourself with the contents of the Instruction Manual before using the instrument for the ﬁrst time.

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Remove the cardboard packing piece 3 and place to one side.

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Remove the two foam packing pieces 4 and place these to one side.

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Grasp the instrument 5 (which will be sealed in a polythene bag) ﬁrmly at each end, and lift out of the carton to place on an adjacent ﬂat, ﬁrm and clean surface.

The instrument weighs approximately 10 Kgs.

PLEASE TAKE CARE WHEN LIFTING.

•

Remove the Accessory carton 6. Note that this contains the Power and USB cables together with any sample cells or other items that may have been ordered. Refer to the Instruction Manual to ensure accessories are installed correctly before using the instrument

for the ﬁrst time.

Disposal of Packaging

It is recommended that the Instrument packaging be retained for possible future long-term storage or transportation. Please note that the Manufacturer or Distributor cannot be held responsible for any damage incurred as a result of transportation of an inadequately packed instrument.

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3

4

5

6

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If you wish to dispose of the instrument packaging, please do so in an environmentally responsible manner. Please refer to the following guidelines: -

Cardboard packing items are made from paper sourced from recycled ﬁbres or managed forests and can be 100% recycled where appropriate facilities exist. Ensure cartons are crushed or ﬂattened before disposal.

Foam packing pieces are manufactured with a reprocessed polyethylene content and can be easily recycled with other low-density polyethylene (LDPE) materials. Polyethylene foam is manufactured by a CFC and HCFC free process and contains less than 100ppm heavy metal content. The European Union Packaging Directive conﬁrms that recovery by means of wasteto-energy is a sensible waste management alternative. The Polyethylene foam burns cleanly and contributes a high caloriﬁc value.

2.2 INSTALLATION

2.2.1 LOCATION

In ideal circumstances the installation environment will be clean, dry and dust free with the instrument protected from extreme variations in ambient lighting and temperature change. Ensure the unit is positioned so that the mains on/off switch is accessible. If a safety problem should be encountered, switch off at the mains socket and remove the plug from the supply.

Where conditions are less than ideal, maintenance and cleaning must be carried out regularly and additional protection offered where possible.

The optional dust cover should be used to protect the instrument when not in use.

2.2.2 SUPPLY VOLTAGE

The 67 series spectrophotometers are powered by a universal switch-mode power supply that operates from a 90-264Vac mains supply. The correct lead for your supply should be selected.

2.2.3 MAINS CONNECTIONS

The leads supplied have a moulded on plug. However, if this is removed for any reason the wires in the mains lead are colour coded to conform to the internationally recognised standard such that:

UK CONNECTIONS US CONNECTIONS

Brown Live Black Live Blue Neutral White Neutral Green/Yellow Earth Green Earth

Safety

When disposing of any removed plug the connectors must be removed or made incapable of insertion into a mains socket.

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2.2.4 TOUCH SCREEN FUNCTIONS

1. Standby – this key can be used to enter the standby mode during operation.

2. Instrument Settings – this key can be used to access instrument set up parameters

including user creation, PIN codes, language options, date and time settings, administrative functions and instrument identiﬁcation details.

3. Back key – this is used to return to a previous menu level.

4. Save key – pressing this key saves methods and/or data. If an external card is not ﬁtted

then methods/data will automatically be saved to internal memory. If an SD card is inserted or removed during operation relevant messages and options are given to select the desired media.

5. Toolbar key – this key is only functional on the completion of measurements and gives access to the available data manipulation options. The function of this key is context sensitive, enabling different sets of tools depending on the mode of operation in use.

6. Printer key – this key will initiate a print out to either the internal or external printer unit via the selected settings options in each mode of operation.

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3

6

5

4

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2.2.5 TOOLBAR ICONS

The spectrophotometer can be used for making measurements at any time without the need for logging in; but many beneﬁts will be lost and results cannot be saved, only printed.

The Method Screen options will only be displayed if the user logs in. If the user is not logged in the instrument will automatically display the main measurement screen, with settings at their last used levels.

Method Screens

Methods are stored sequentially by measurement mode. Once the ﬁrst page is full (8 methods for the selected mode) cursor arrows are displayed enabling the user to browse to subsequent or previous pages of 8 methods.

Alternatively, pressing one of the alphanumeric keys along the bottom of the screen will display all available methods with the initial character that is highlighted.

Note: Repeated pressing of a key sequentially highlights the characters between those displayed.

Create a New Method

For the following functions ﬁrst touch a method or result to select it.

Open the Selected Method

Erase the Selected Method/Result

Browse Results – linked to the selected method

Open speciﬁc results in the selected batch

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Photometrics Mode

Mode settings – method name, wavelength, method security (if logged in)

Accessory options – varies with type of accessory module ﬁtted

Allows selection of internal or external printer

Allows set-up of batch ID and the Auto Log options

Press to accept settings entered

Spectrum Mode

Mode settings – method name, measurement mode, wavelength range, plot interval, method security (if logged in)

Analysis Points – up to 30 wavelengths at which absorbance will be reported

Measurement display – auto scaling, axis setting and colour selection

Accessory options – varies with type of accessory module ﬁtted

Allows selection of internal or external printer, graph details, batch ID and enables or disables the Auto Log feature

Press to accept settings entered

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Multi-Wavelength Mode

Mode settings – method name, measurement type, reading type, sample style, method security (if logged in)

Setting wavelengths – allows from 2 to 4 wavelengths to be set

Calculations - allows the selection of calculations and constants

Accessory options – varies with type of accessory module ﬁtted

Allows selection of internal or external printer, graph details, batch ID and the Auto Log options

Press to accept settings entered

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Kinetics Mode

Mode settings - method name, wavelength, measurement mode, run settings, method security (if logged in)

Calibration – allows setting of Concentration cal standard, factor, resolution and units of measurement

Measurement display – allows auto scaling, axis setting and colour selection

Accessory options – varies with type of accessory module ﬁtted

Allows selection of internal or external printer, graph details, batch ID and the Auto Log options

Press to accept settings entered

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Quantitation Mode

Mode settings – method name, measurement mode, wavelength, resolution, units of measurement, replicates set up, method security (if logged in)

Calibration – allows the number and levels of standards to be set

Measurement display – allows selection of type of curve ﬁt, auto scaling, axis setting and colour selection

Accessory options – varies with type of accessory module ﬁtted

Allows selection of internal or external printer, graph details, batch ID and the Auto Log options

Press to accept settings entered

Status Bar

To view the set parameters in any of the measurement modes press the status bar once and a drop down menu will appear. Pressing this bar again will return it to its original status.

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2.2.6 REAR PANEL LAYOUT

Power Switch On/Off switch for the unit.

Power In Socket IEC type connection socket for the mains supply cable.

Printer Socket 25 way Centronics parallel output socket compatible with Postscript printers.

Analogue Output 2 x 4mm pin sockets for analogue devices.

USB Socket type B connector for communication to PC.

SD Card Socket will accept 128MB to 2GB SD or Multi-media memory cards.

Printer socket

USB SocketAnalogue O/P SD Card Socket

Power switch

Power in socket

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2.2.7 POWER ON AND SELF-TESTS

response

Dark test… Checks detector and light seal on sample chamber

Wavelength calibration… Performs a wavelength calibration

followed by the Main Menu Screen:

This screen gives you access to the ﬁve operating modes that can be used directly for making measurements without logging in. It should be noted that if the user is not logged in results can only be viewed and printed but not stored. In addition, methods cannot be created or retrieved.

Note: For the Model 6700 allow 30 minutes warm up prior to use.

For all models the LCD display may take up to 10 minutes to reach full brightness.

Connect the mains supply cable to the rear panel mains input socket and plug the other end into a suitable mains supply socket.

Lift the sample chamber lid on the instrument and ensure that there is no sample or other item present in the sample holder, close the lid.

Switch on the supply socket, then the instrument, using the Power switch located on the rear panel.

The instrument will then perform the power on self-test protocol. The power on and self-test screens will be shown:

System test… Checks internal connections / Checks internal and external memory status

Accessory test… Checks for ﬁtted ‘active’ accessories / Veriﬁes communication and

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SECTION 3 - Systems of Operation

3.1 MULTI-USER AND FREE OPERATION

The 67 Series spectrophotometers focus on secure, multi-user operation. But to ensure that anyone

can acquire quality results in an emergency or when otherwise required (stat or free operation), they may also be used without the operator having to log in. Such free operation is restricted to making measurements with no access to methods while results will only be displayed or printed.

Secure, multi-user operation requires each designated user to enter a PIN code to access methods and results. When creating methods a user can opt to share them with other users, as Public (can be modiﬁed) or Read-Only (can be used but not modiﬁed), or to keep them Personal. Up to 10 users and a Supervisor with administrative rights can be set up on each instrument but by using the removable media (see below) an almost inﬁnite number of users can be securely accommodated.

3.2 MEMORY STRUCTURE

The 67 Series spectrophotometers have a dual memory structure based on removable media and ﬁxed internal memory. The removable media is based on SD memory cards with a wide range of

capacities available.

The following table gives details of the removable media available (subject to change by manufacturers range consolidation):

Card Capacity

2 GB

3.2.1

MEMORY SELECTION

When the power is turned on the internal memory is selected as the default memory location, even if a removable memory card is ﬁtted in the instrument. If the user inserts a removable media memory card during use a message will be displayed giving the user the option to switch the default memory location to the removable media memory card so that any methods and results that are saved are sent to this card. Should the card be removed during use a warning message will be displayed and the internal memory will then be set as the default location.

SD Card Part Number

019 133

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3.2.2 MEMORY HIERARCHY

The top level of the memory hierarchy is the user, deﬁned by a user name and recognised by their unique PIN code. In general it is considered that this will be an individual but in practice this can be made more ﬂexible by considering the user as a group sharing the same PIN code. For instance different departments or shifts could be identiﬁed by their own PIN codes.

Different types of tests could also be separated by PIN codes with only users trained in carrying out speciﬁc tests given the relevant PIN codes to access the tests for which they have been trained. In education a PIN code for each semester or part of the curriculum would restrict students or year groups access to only the current work, protecting that to be used in the future. The implementation and beneﬁts of this function are only limited by the imagination.

Below the user level are the Methods created by that user or for that group, and those with Public or Read-Only status that all users can access, below each method are the results stored by the user for THAT METHOD.

3.3 CREATING METHODS

The ability to create and re-call methods is only available to logged in users.

To log in touch the Log In… key on the bottom right-hand side of the Main Menu screen that is displayed after completion of the start-up tests.

A table of users is then displayed, touching your name (department, user group or other identiﬁer) then brings up a numeric entry screen for inputting the four digits of the relevant PIN code.

For ﬁrst time use the only user in the table will be the Supervisor with a default PIN Code of 1234 (once logged in this can subsequently be changed to a number combination of the Supervisor’s choice).

By pressing the Settings key on the display surround and selecting the Administrative Functions option the Supervisor can create new users. On creation a new user has a default PIN Code of 0000 that, like the Supervisor’s, can be changed by selecting User Preferences, then Adjust PIN Code.

NOTE: Once the Supervisor’s PIN CODE is changed it must be kept safe as there is no other way of accessing this function – however the Supervisor does have the right to re-set other users’ PIN Codes back to the default.

For further information on these functions see the Section on Instrument Settings and Administrative Functions.

Read-Only

Methods

User deﬁned

by PIN Code

Public

Methods

Created by User

Results appended

to user accessible

methods

25

When successfully logged in the user is returned to the main menu screen from which the required mode of measurement can be selected.

The Browse screen is then displayed from which an existing method can be re-called using the alphanumeric search keys along the bottom menu bar. If insufﬁcient methods have been created to ﬁll the ﬁrst page all available methods will be shown.

3.4 FILE & DATA MANAGEMENT

Save Tools Print Settings Erase Search Open Save Results Results

Saving Methods

Having entered all your required settings on the tabbed pages the method can be saved by simply pressing the Save key on the display surround. If you do not save at this point but continue to make measurements using the method, it will automatically be saved when you save the ﬁrst result.

If you continue without saving a result you will be prompted to save the method as you exit the operating mode or return to the settings options. Alternatively, when the Auto Log option is set to On or Timed and the destination option is Memory all results will be saved automatically.

Sharing Methods

Methods can be shared with other users by setting the security level at either Read-Only where other users can use but not modify the method or, Public where they can use and modify the method. Other users must then ensure that under Method View Settings (Settings/User Preferences/Method View Settings) they have enabled Public and/or Read-Only methods or turned All Methods on.

Recalling Methods

Following selection of the operating mode from the main menu the method browse screen is displayed. This will show all methods that the current user has access to, based on their selections in the Method View Settings (refer to Sharing Methods). If the ﬁrst page is full, cursor arrows will be displayed to enable navigation to and from additional pages.

Alternatively, selection may be made using the alphanumeric menu bar at the bottom of the screen.

Repeated pressing of each character set will display the full alphanumeric range and the screen will show all methods starting with the highlighted character.

Touch the required method when it is displayed on the screen to highlight it, touch the Open File icon to display the main measurement screen for that method.

Editing Methods

Use the Recalling Methods procedure to recall the required method. With the measurement screen displayed touch the Settings option. Adjust the settings as required and touch the Enter icon on completion. The modiﬁed method can then be saved by pressing the Save key on the display surround. If you do not save at this point but continue to make measurements using the method, it will automatically be saved when you save the ﬁrst result. If you continue without saving a result you will be prompted to save the method as you exit the operating mode or return to the settings options.

Note: If the Method name was not changed during editing it will be saved with the same name but with a new date and time to ensure traceability. If the old method is no longer required it should be deleted as detailed in Deleting Methods.

26

Deleting Methods

To delete methods highlight the required method in the Browse screen as described in Recalling Methods and then touch the Erase icon.

A warning message will be displayed to ensure this action is required. On conﬁrmation the selected ﬁle will be deleted.

If the current user does not have the required privileges to delete the selected method then an information message will be displayed advising that the method cannot be deleted.

Privileges required for deleting designated methods:

Public Methods – only the Supervisor and Originator can delete these.

Read-Only Methods – only the Supervisor and Originator can delete these.

Personal Methods – Only the Originator can delete these methods. (The Supervisor can delete these by re-

setting the Originator’s PIN code and then logging in as the Originator).

Saving Results

After completion of a measurement the result can be saved by simply pressing the Save key on the display surround. The result is saved under the method that created it, with the entered Batch ID and an incremental number along with the time and date of the measurement. Results can also be saved as part of the Auto Log function by selecting Memory under the Auto Log option, which will vary depending on the type of sampling accessory ﬁtted.

Printing Results

After completion of a measurement the result can be printed, by simply pressing the Print key on the display surround. The result will be printed to either the internal or external printer, as selected by the user in the Printer Settings option.

The ﬁrst result of any new batch is preceded by a print header, which gives details of the method settings and Batch ID. Results can also be printed as part of the Auto Log function, which will vary depending on the type of sampling accessory ﬁtted.

Recalling Results

Stored results are always directly linked to the method that created them. To access results ﬁrst recall the method as described in Recalling Methods. With the method highlighted touch the Search Results icon. This will open a screen detailing all results available to the current user. Touch the required result or batch (depending on the mode) and then the Open Speciﬁc Result icon. This will display the results on the screen. The Tools option can then be used to work on these results (depending on mode). It is also possible to print the result by simply pressing the Print key on the display surround. Options to print to the Internal or External printer or to the CSV ﬁle will be displayed. Printing to the CSV (Comma Separated Values) ﬁle will save the data in CSV format on the external data card. (If a card is not ﬁtted the instrument will display a prompt). This is in text format and can be viewed and printed in Excel

®

.

In Kinetics mode an additional option is available (Analogue Mode). This allows results to be output via the rear panel analogue sockets to a chart recorder or similar device.

Sharing Results

Results attached to Personal Methods cannot be accessed by any other user.

Results attached to Read-Only and Public Methods can be accessed by all users, based on their current Method View Settings.

27

SECTION 4 - Instrument Settings

4.1 MENU OPTIONS

A number of settings can be stored for each signed in user including language, method view settings, display brightness and time/date display choices so that these do not have to be reset each time a new user logs in. These can be found under User Preferences.

Note: Administration Functions and Memory Manager options will be greyed out and unavailable unless the Supervisor has logged in.

– When switching on the product for the ﬁrst time, or if the previously entered settings need to be checked, this key should be selected to give access to the following display:

4.2 SET TIME & DATE

To set the correct date and time select the Set Time & Date key. The time and date can be reset using the up and down arrows. To accept the new settings select the Enter key. Touching the Cancel key will return you to the main menu without accepting any alterations to the current settings.

4.3 USER PREFERENCES

Selecting User Preferences gives access to the following options:

These settings are saved for each user and the individual’s selections are retained when that user logs in. Adjust User PIN and Method View Settings options are only available to the Supervisor.

Note: Adjust User PIN and Method View Settings will only be available to the Supervisor.

28

4.3.1 ADJUST USER PIN – available to logged in users only

To alter your User PIN select the Adjust User PIN key. A warning message will be shown prior to the numeric keypad. Enter your new 4 digit code using the numeric keypad as shown below. The Del key allows individual digits to be changed. The Cancel key will return you to the previous menu without altering the original PIN code. The Clear key will clear all digits entered from the screen. The Enter key accepts the new code and returns you to the previous menu.

4.3.2 LANGUAGE

The preferred language can be selected using the Language key. Select the preferred language from the options available and press the OK key and the will update to show all screens in English, French, German, Italian or Spanish.

4.3.3 METHOD VIEW SETTINGS – available to logged in users only

To simplify operation it is possible to restrict the methods displayed by their allocated category. Selecting Method View Settings enables My Methods (Personal) to be hidden (Off) or displayed (On). Similarly, Read Only Methods and Public Methods can be hidden or displayed. To simply display all method categories toggle All Methods to the On status.

Please ensure you remember your PIN code or store it in a secure location, or access to your personal data will be lost.

OK

29

4.3.4 TOUCH SCREEN CLICK

The touch screen can be operated with or without an audible click each time a key is touched. The click can be toggled on or off via the Touch Screen Audible Click: Off key.

4.3.5 BRIGHTNESS

Selecting the Brightness key and then using the left and right arrows on the display shown below can adjust the brightness of the display. Selecting the Enter key will update the display setting and return the instrument to the previous display.

4.3.6 ADJUST DATE & TIME

Selecting the Date & Time Preferences key allows the user to display either the time or date. In addition the date format can be set to either DD/MM/YY or MM/DD/YY. Once the preferred option is selected, pressing the Back key on the display surround once will return you to the previous menu. Pressing the Back key twice returns the instrument to the Main Menu.

30

4.4 ABOUT

Selecting the About key provides information relating to instrument ID and signed in user details. Selecting the OK key returns the instrument to the previous menu.

4.5 FORM FEED

Selecting the Form Feed key will feed additional paper through the mechanism of the connected printer to separate results or enable a clean area for tearing off.

4.6 ADMINISTRATION FUNCTIONS

This function is only available to the Supervisor. It gives access to all user related information. It gives access to Instrument ID, screen dump control and Modify Users sub-menu.

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4.6.1 INSTRUMENT ID

Selecting the Instrument Id: 67XXCVS key allows the supervisor to alter the instrument identiﬁcation code. This is entered by use of the alphanumeric keypad. The Cancel key will return you to the previous menu without altering the original ID. The Del key allows individual letters or digits to be removed. The Clear key will clear all digits entered from the screen. The Enter key accepts the new ID and returns you to the previous menu. For laboratories with more than one 67 series spectrophotometer it is useful to change this ID to the serial number, department or other unique identiﬁer for easy recognition of different instruments.

4.6.2 SCREEN DUMP

The screen dump function can be enabled or disabled.

4.6.3 MODIFY USERS

This gives access to the sub-menu which allows the supervisor to modify user parameters.

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4.6.3.1 CREATE NEW USER

Selecting the Create New User key allows the supervisor to enter an additional user on to the instrument using the alphanumeric keypad as shown. The Clear key will clear all information entered from the screen. The Del key allows individual letters or digits to be removed. The Cancel key will return you to the previous menu without adding a new user. The Enter key accepts the new ID and returns you to the previous menu.

4.6.3.2 DELETE USER

Selecting the Delete User option allows the supervisor to delete a speciﬁc user from the instrument. When the user name is selected from the list the following warning message is displayed:

If Yes is selected the instrument will update and the user will be removed from the instrument.

If Cancel is selected the user will not be deleted from the instrument.

4.6.3.3 DELETE ALL USERS

Selecting this option allows the supervisor to delete all current users (except the supervisor) from the instrument. The following warning message is displayed:

If Yes is selected the instrument will update and all users will be removed from the instrument. If Cancel is selected all users will remain on the instrument.

6715 CVS

All users will be permanently deleted from the instrument. Are you sure you wish to continue?

Yes Cancel

6715 CVS

This user will be permanently deleted from the instrument. Are you sure you wish to continue?

Yes Cancel

33

4.6.3.4 RESET USER PIN

This option allows the supervisor to reset an individual users PIN code.

Select the Reset User PIN option from the screen. The list of current users will then be displayed on screen. Select the user. The warning message: ‘Are you sure you wish to continue’ will be displayed with the options to accept or cancel.

If Yes is selected the user PIN will be reset to the default value and the supervisor has access to all information relating to that user.

If Cancel is selected no changes will be made to the user codes and the instrument will return to the Select User Menu.

4.7 MEMORY MANAGER

This option is available only to the Supervisor and allows transfer of data, methods and user preferences to and from the instrument memory. This enables backup of information stored in the instrument memory and on any removable media used. Both memory locations can also be formatted.

WARNING – this will permanently delete all information stored in the location being formatted.

34

SECTION 5 - Photometrics Mode

5.1 PRINCIPLES OF MEASUREMENT

The simplest mode of the spectrophotometer is Photometrics. A measurement is made of either the absorbance or transmittance of a sample. The measurement is at a single wavelength, at one point in time, with no additional calculations.

Select Photometrics from the Main Menu:

If the user is not logged in (Free Operation) the main measurement screen will be displayed (refer Section 5.2).

5.2 FREE OPERATION

Settings

If the user is not logged in then the screen will show the following message when the Photometrics Mode key is selected from the Main Menu:

If Yes is selected the instrument will go to the main measurement screen.

If No is selected the instrument will return to the main menu where the user has the option to Log In.

As you are not logged in you will not be able to save results. Are you sure you want to continue?

Yes No

35

To enter the appropriate parameters for the sample(s) to be tested select the Settings key and the instrument will display the following screen:

Mode settings - method ID, wavelength, method security (if logged in)

Accessory options - varies with type of accessory module ﬁtted

Allows selection of internal or external printers

Allows set-up of batch ID and enables or disables or disables the Auto Save feature

Press to accept settings entered

Setting Method ID

To allocate a Method name select the Method key and enter the preferred name using the alphanumeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual letters or digits to be removed. The Cancel key will return you to the previous menu and the method name will remain as Default Method. The Enter key accepts the new method ID and returns you to the previous menu.

Note: The new Method ID can only be used to identify the method on a printout to the internal or external printer module. The generic user has no additional facility to store or recall results or methods.

36

Setting Wavelength

Select the Wavelength key and enter the wavelength using the numeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual digits to be removed. The Cancel key will return you to the previous menu and the wavelength will not alter. The Enter key accepts the new wavelength and returns you to the previous menu. (At certain wavelengths order selecting ﬁlters may be heard switching in or out). Select the Enter key to conﬁrm and the display will update to show the new wavelength.

Using the Back key to escape settings will display:

If Yes is selected all set information will be lost and the instrument will return to the main measurement screen.

If No is selected the instrument returns the Settings screen as shown.

To accept all entered information touch the Enter key to conﬁrm. The instrument will show the main measurement screen with the updated information.

Setting Method Security

This option is only applicable to logged in users.

Batch ID and Auto Logging can be entered via the Save icon.

Setting Batch ID

Select the Batch ID key and enter the Batch code using the alphanumeric keypad. Select the Enter key to accept or Cancel to remain as a default.

6715 CVS

Continue? , if so you will lose any changes you have made.

Yes Cancel

37

Auto Log Settings…

Selecting Auto Log Settings… opens a dedicated sub-menu.

This option can be toggled between On, Off or Timed.

Auto Log Settings

This option can be toggled between Off/On/Timed. When Auto Log option is selected to On the user can set:

the destination, (toggles between internal or external printer and memory).

When the Auto Log option is set to Timed the user can set:

the timed interval between 3 and 9999 seconds using the numeric keypad;

the destination (toggles between internal or external printer and memory);

the number of repetitions from 2 to 999 using the numeric keypad.

To view the set parameters touch the status bar once and a drop down menu will appear giving this detail. Touching the bar again will return it to its original position.

Note: If the Auto Log Timed option is selected the Read key will be replaced with Start/Stop keys.

38

5.3 LOGGED IN MEASUREMENT

PIN Codes – each user is allocated a 4 digit PIN code that is required when logging in.

Select Log In… from the Main Menu and a list of users will be shown. Select the appropriate user name and a numeric keypad will be displayed.

Enter your 4 digit PIN code and touch the Enter key to conﬁrm.

Once logged in method screen options will be displayed:

Methods are stored sequentially by measurement mode. Once the ﬁrst page is full (8 methods for the selected mode) cursor arrows are displayed in the top corners enabling the user to browse to subsequent or previous pages of 8 methods.

Alternatively, pressing one of the alphanumeric keys along the bottom of the screen will display all available methods with the initial character that is highlighted.

Note: Repeated pressing of a key sequentially highlights the characters between those displayed.

Create a New Method

For the following functions ﬁrst touch a method or result to select it.

Open the Selected Method

Erase the Selected Method

Browse Results – linked to the selected method

Open speciﬁc results in the selected batch

39

Creating a New Method

Settings

To enter the Method ID, appropriate wavelength for the sample(s) to be tested and the level of Method Security required for the method being created select the New File icon

Setting Method ID

To allocate a Method name select the Method key and enter the preferred name using the alphanumeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual letters or digits to be removed. The Cancel key will return you to the previous menu and the method name will remain as Default Method. The Enter key accepts the new method ID and returns you to the previous menu.

Setting Wavelength

Select the Wavelength key and enter the wavelength using the numeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual digits to be removed. The Cancel key will return you to the previous menu and the wavelength will not alter. The Enter key accepts the new wavelength and returns you to the previous menu. (At certain wavelengths order selecting ﬁlters may be heard switching in or out). Select the Enter key to conﬁrm and the display will update to show the new wavelength.

Using the Back key to escape settings will display:

If Yes is selected all set information will be lost and the instrument will return to the main measurement screen. If No is selected the instrument returns the Settings screen as shown.

6715 CVS

Continue? , if so you will lose any changes you have made.

Yes Cancel

40

Setting Method Security

The 67 Series spectrophotometers can support up to 10 individual users plus one Supervisor who has full administrative rights.

Logged in users can create methods with three levels of security options:

Personal – these methods are only accessible by the originator.

Public – these methods are available for use and modiﬁcation by any logged in user.

Read-Only – these methods can be accessed by all logged in users, but can only be modiﬁed by the

originator.

The preferred level of protection can be achieved by selecting the Method Security key that toggles between Personal, Public and Read-Only options.

To accept the selected options touch the Enter key to conﬁrm. The instrument will show the main measurement screen with the updated information.

Setting Batch ID

Select the Batch ID key and enter the batch code using the alphanumeric keypad. Select Enter to accept or Cancel to remain as a default.

41

Auto Log Settings…

Selecting Auto Log Settings… opens a dedicated sub-menu.

This option can be toggled between On, Off or Timed.

Auto Log Settings

This option can be toggled between Off/On/Timed. When Auto Log option is selected to On the user can set:

the destination, (toggles between internal or external printer and memory).

When the Auto Log option is set to Timed the user can set:

the timed interval between 3 and 9999 seconds using the numeric keypad;

the destination (toggles between internal or external printer and memory);

the number of repetitions from 2 to 999 using the numeric keypad.

To view the set parameters touch the status bar once and a drop down menu will appear giving this detail. Touching the bar again will return it to its original position.

Note: If the Auto Log Timed option is selected the Read key will be replaced with Start/Stop keys.

42

File & Data Management

Save Tools Print Settings Erase Search Open Save Results Results

Saving Methods

Having entered all your required settings on the tabbed pages the method can be saved by simply pressing the Save key on the display surround. If you do not save at this point but continue to make measurements using the method, it will automatically be saved when you save the ﬁrst result.

If you continue without saving a result you will be prompted to save the method as you exit the operating mode or return to the settings options. Alternatively, when the Auto Log option is set to On or Timed and the destination option is Memory, all results will be saved automatically.

Sharing Methods

Methods can be shared with other users by setting the security level at either Read-Only where other users can use but not modify the method or, Public where they can use and modify the method. Other users must then ensure that under Method View Settings (Settings/User Preferences/Method View Settings) they have enabled Public and/or Read-Only methods or turned All Methods on.

Recalling Methods

Following selection of the operating mode from the main menu the method browse screen is displayed. This will show all methods that the current user has access to, based on their selections in the Method View Settings (refer to Sharing Methods). If the ﬁrst page is full, cursor arrows will be displayed to enable navigation to and from additional pages. Alternatively, selection may be made using the alphanumeric menu bar at the bottom of the screen.

Repeated pressing of each character set will display the full alphanumeric range and the screen will show all methods starting with the highlighted character.

Touch the required method when it is displayed on the screen to highlight it, touch the Open File icon to display the main measurement screen for that method.

Editing Methods

Use the Recalling Methods procedure to recall the required method. With the measurement screen displayed touch the Settings option. Adjust the settings as required and touch the Enter icon on completion. The modiﬁed method can then be saved by pressing the Save key on the display surround. If you do not save at this point but continue to make measurements using the method, it will automatically be saved when you save the ﬁrst result. If you continue without saving a result you will be prompted to save the method as you exit the operating mode or return to the settings options.

Note: If the Method name was not changed during editing it will be saved with the same name but with a new date and time to ensure traceability. If the old method is no longer required it should be deleted as detailed in Deleting Methods.

Deleting Methods

To delete methods highlight the required method in the Browse screen as described in Recalling Methods and then touch the Erase icon. A warning message will be displayed to ensure this action is required. On conﬁrmation the selected ﬁle will be deleted.

If the current user does not have the required privileges to delete the selected method then an information message will be displayed advising that the method cannot be deleted.

43

Privileges required for deleting designated methods:

Public Methods – only the Supervisor and Originator can delete these.

Read-Only Methods – only the Supervisor and Originator can delete these.

Personal Methods – Only the Originator can delete these methods. (The Supervisor can delete these

by re-setting the Originator’s PIN code and then logging in as the Originator).

Saving Results

After completion of a measurement the result can be saved by simply pressing the Save key on the display surround. The result is saved under the method that created it, with the entered Batch ID and an incremental number along with the time and date of the measurement. Results can also be saved as part of the Auto Log function by selecting Memory under the Auto Log option, which will vary depending on the type of sampling accessory ﬁtted.

Printing Results

After completion of a measurement the result can be printed, by simply pressing the Print key on the display surround. The result will be printed to either the internal or external printer, as selected by the user in the Printer Settings option.

The ﬁrst result of any new batch is preceded by a print header, which gives details of the method settings and Batch ID. Results can also be printed as part of the Auto Log function, which will vary depending on the type of sampling accessory ﬁtted.

Recalling Results

Stored results are always directly linked to the method that created them. To access results ﬁrst recall the method as described in Recalling Methods. With the method highlighted touch the Search Results icon. This will open a screen detailing all results available to the current user. Touch the required result or batch (depending on the mode) and then the Open Speciﬁc Result icon. This will display the results on the screen. The Tools option can then be used to work on these results (depending on mode). It is also possible to print the result by simply pressing the Print key on the display surround. Options to print to the Internal or External printer or to the CSV ﬁle will be displayed. Printing to the CSV (Comma Separated Values) ﬁle will save the data in CSV format on the external data card. (If a card is not ﬁtted the instrument will display a prompt). This is in text format and can be viewed and printed in Excel

®

.

In Kinetics mode an additional option is available (Analogue Mode). This allows results to be output via the rear panel analogue sockets to a chart recorder or similar device.

Sharing Results

Results attached to Personal Methods cannot be accessed by any other user.

Results attached to Read-Only and Public Methods can be accessed by all users, based on their current Method View Settings.

5.4 PERFORMING MEASUREMENTS – all users

Once the required settings have been conﬁrmed the main Photometrics measurement screen will be shown. This screen shows the absorbance value, followed by the Transmittance value and then the selected wavelength. At the top of the screen Method ID and Batch number, time or date is displayed.

Zeroing the Instrument

Insert a cuvette containing a blank solution into the sample holder and close the sample chamber lid. (Test tubes or other sample containers may be used depending on the sample holder accessory ﬁtted). Select the Zero key. The messages Calibrating Dark Level… and Calibrating Light Level...’ are displayed momentarily. Once the measurement is completed the screen will update to show:

Note: In general the blank solution should contain everything that is in the sample except the colour-producing component. For speciﬁc information reference should be made to the procedure or application being followed. For enhanced reproducibility matched cuvettes should be used.

Measuring the Sample

Remove the cuvette containing the blank solution and insert the ﬁrst sample into the sample holder and close the sample chamber lid. Select the Read key.

The screen will momentarily display ‘Taking reading. Please wait…’ Once the measurement is completed the screen will update to show the measured value.

Additional individual samples can be measured by inserting them in the single sample holder and selecting Read. Alternatively, multiple samples can be measured with the optional 8 or 6 cell changers

or sequentially with the sipper pump accessory.

44

45

Section 6 - Spectrum Mode

6.1 PRINCIPLES OF MEASUREMENT

By examining the absorbance or transmittance of the sample over a wavelength range we can partially characterise the sample. Readings of the sample over a range of wavelengths are made and a graph is plotted of the absorbance or transmittance at each wavelength. As well as observing good practice (see Good Practice Guidelines) it must be ensured that the sample is stable for the period of the scan (maximum 70 seconds).

Select the Spectrum Mode from the Main Menu options.

The method screen options will only be displayed if the user logs in. If the user is not logged in the instrument will automatically display the main instrument screen, with settings at their last used levels.

6.2 FREE OPERATION

Settings

If the user is not logged in then the main measurement screen will automatically be displayed when the Spectrum mode is selected from the Main Menu:

To enter the required parameters for the sample(s) under test, select the Settings key and the instrument will display the following screen:

46

Mode settings – method name, measurement mode, wavelength range, plot interval, method security (if logged in)

Analysis Points – up to 30 wavelengths at which absorbance will be reported

Measurement display – auto scaling, axis setting, colour selection

Accessory options – varies with type of accessory module ﬁtted

Allows selection of internal or external printer, graph details, batch ID and enables or disables the Auto Log feature

Press to accept settings entered

Setting Method ID

To allocate a Method name select the Method key and enter the preferred name using the alphanumeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual letters or digits to be removed. The Cancel key will return you to the previous menu and the method name will remain as Default Method. The Enter key accepts the new method ID and returns you to the previous menu.

Note: The new Method ID can only be used to identify the method on a printout to the internal or external printer module. The generic user has no additional facility to store or recall results or methods.

Selecting Measurement Mode

The Measurement Mode key toggles between Absorbance and Transmittance.

47

Setting Wavelengths

Note: If the start or end wavelength is adjusted so that they are closer than twice the plot interval

then the previously set wavelength will be automatically adjusted to ensure a minimum of two measurement points.

Setting Start Wavelength

Select the Start Wavelength key and enter the wavelength using the numeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual digits to be removed. The Cancel key will return you to the previous menu and the wavelength will not alter. The Enter key accepts the new wavelength and returns you to the previous menu. (At certain wavelengths order selecting ﬁlters may be heard switching in or out). Select the Enter key to conﬁrm. The display will show the entered wavelength.

Setting End Wavelength

Select the End Wavelength key and enter the wavelength using the numeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual digits to be removed. The Cancel key will return you to the previous menu and the wavelength will not alter. The Enter key accepts the new wavelength and returns you to the previous menu. (At certain wavelengths order selecting ﬁlters may be heard switching in or out). Select the Enter key to conﬁrm. The display will show the entered wavelength.

48

Selecting Plot Interval

Selecting the Plot Interval key allows the user to toggle between 1.0nm, 5.0nm, 0.1nm and 0.5nm.

Using the Back key to escape settings will display:

If Yes is selected all set information will be lost and the instrument will return to the main measurement screen. If No is selected the instrument returns the Settings screen as shown.

To accept the entered information at any time, select the Enter key to conﬁrm. The instrument will show the main measurement screen with the updated information.

To view the set parameters touch the status bar once and a drop down menu will appear. Touching this bar again will return it to its original status.

6715 CVS

Continue? , if so you will lose any changes you have made.

Yes Cancel

49

Setting Method Security

This option is only applicable to logged in users.

Setting Additional Set-up Parameters using the Toolbar Icons

Setting Analysis Points

Up to 30 analysis points can be set be selecting the Analysis Points keys and entering the required wavelength value via the numeric keypad.

It should be noted that a warning message will be displayed if the analysis point(s) set are outside the spectrum scan range and a warning symbol will be shown next to the entered value.

The Clear Analysis Points option will clear all previously set analysis points.

To delete or edit individual analysis points select the required analysis point to display:

If Del is selected the analysis point is deleted.

If Edit is selected a new value can be entered via the numeric keypad.

If an attempt is made to set an analysis point outside the start and end wavelength values the error message ‘Wavelength for analysis out of spectrum scan range’ will be displayed momentarily and a triangular symbol will be shown to the side of the incorrect value. This value can be deleted or edited by selecting the analysis point (in this example 1) and entering a new value within the speciﬁed limits.

The analysis points will appear on tags at the relevant point on the spectrum display. They will also be printed with any printout to the internal or external printer if the Print Data Points option has been selected.

6715 CVS

What would you like to do to this analysis point?

Del Edit

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Setting Auto-Scale

The Auto-Scale key toggles between On and Off. With the Auto-Scale function On the instrument will automatically set the Y-axis maxima to a level that will ﬁt the largest peak and lowest valley in the display area. This is the best option to choose if the user is uncertain of the limits of the scan.

When set to On the manual settings for Y-axis maximum and minimum are non-functional. These settings can be changed with Post Scan analysis tools to enable alternative views and printouts after the scan has been completed.

With the Auto-Scale function set to Off the Y-Axis Minimum enables the lowest displayed level for the Y-axis to be manually set. Select the Y-Axis Minimum key and a numeric keypad will be displayed for entry of this value. This value can be between –0.301 and 2.999.

The Y-Axis Maximum enables the highest displayed level for the Y-axis to be manually set. Select the Y-Axis Maximum key and a numeric keypad will be displayed for entry of this value. This value can be between 3.000 and –0.300.

Selecting Plot Colour

Selecting the Plot Colour key displays a colour selection screen. Touch any one of the eight colours to select the preferred plot colour. Once selected, the screen will update to show the selected colour.

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Selecting Axis Colour

Selecting the Axis Colour key displays a colour selection screen. Touch any one of the eight colours to select the preferred colour. Once selected, the screen will update to show the selected colour.

Setting Batch ID

Select the Batch ID key and enter the batch code using the alphanumeric keypad. Select Enter to accept or Cancel to remain as a default.

Setting Graph Data Points

The option to print graphs on a full page is only available if the External printer is selected. If available and set to On the scan will be printed on one page (typically A4 landscape format) with the data on following pages.

If available and set to Off the scan will be printed with the data below it (typically top half A4 portrait).

The Print Graph Data Points key toggles between On and Off.

With this key set to On all the data points that make up the graph will be printed in a table following the scan.

Note: For a long scan at 0.1nm resolution this can result in many pages of data. It is recommended that careful consideration should be given to this and that this feature should be set to the Off position in this instance.

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Selecting Printer Media

The Printer Media options toggle between Internal and External Printers.

Auto Log Settings…

Selecting Auto Log Settings… opens a dedicated sub-menu.

This option can be toggled between Off, On and Timed.

When the Auto Log option is selected to On the user can set:

the destination, (toggles between internal or external printer and memory).

When the Auto Log option is set to Timed the user can set:

the timed interval between 3 and 9999 seconds using the numeric keypad;

the destination, (toggles between internal or external printer and memory);

the number of repetitions from 2 to 999 using the numeric keypad.

Selecting the Enter key accepts the entered information and returns the instrument to the main measurement screen.

To view the set parameters touch the status bar once and a drop down menu will appear. Touching this bar again will return it to its original status.

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6.3 LOGGED IN MEASUREMENT

PIN Codes – each user is allocated a 4 digit PIN code that is required when logging in.

Select Log In… from the Main Menu and a list of users will be shown. Select the appropriate user name and a numeric keypad will be displayed.

Enter your 4 digit PIN code and touch the Enter key to conﬁrm.

Once signed in method screen options will be displayed:

Methods are stored sequentially by measurement mode. Once the ﬁrst page is full (8 methods for the selected mode) cursor arrows are displayed in the top corners enabling the user to browse to subsequent or previous pages of 8 methods.

Alternatively, pressing one of the alphanumeric keys along the bottom of the screen will display all available methods with the initial character that is highlighted.

Note: Repeated pressing of a key sequentially highlights the characters between those displayed.

Create a New Method

For the following functions ﬁrst touch a method or result to select it.

Open the Selected Method

Erase the Selected Method

Browse Results – linked to the selected method

Open speciﬁc results in the selected batch

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Creating a New Method

To enter the Method ID, appropriate measurement parameters and the level of Method Security required for the method being created select the New File icon.

Setting Method ID

To allocate a Method name select the Method key and enter the preferred name using the alphanumeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual letters or digits to be removed. The Cancel key will return you to the previous menu and the method name will remain as Default Method. The Enter key accepts the new method ID and returns you to the previous menu.

Selecting Measurement Mode

The Measurement Mode key toggles between Absorbance and Transmittance.

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Setting Wavelengths

Note: If the start or end wavelength is adjusted so that they are closer than twice the plot interval then

the previously set wavelength will be automatically adjusted to give a minimum of two measurement points.

Setting Start Wavelength

Select the Start Wavelength key and enter the wavelength using the numeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual digits to be removed. The Cancel key will return you to the previous menu and the wavelength will not alter. The Enter key accepts the new wavelength and returns you to the previous menu. (At certain wavelengths order selecting ﬁlters may be heard switching in or out). Select the Enter key to conﬁrm. The display will show the entered wavelength.

Setting End Wavelength

Select the End Wavelength key and enter the wavelength using the numeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual digits to be removed. The Cancel key will return you to the previous menu and the wavelength will not alter. The Enter key accepts the new wavelength and returns you to the previous menu. (At certain wavelengths order selecting ﬁlters may be heard switching in or out). Select the Enter key to conﬁrm. The display will show the entered wavelength.

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Selecting Plot Interval

Selecting the Plot Interval key allows the user to toggle between 1.0nm, 5.0nm, 0.1nm and 0.5nm.

Using the Back key to escape settings will display:

If Yes is selected all set information will be lost and the instrument will return to the main measurement screen. If No is selected the instrument returns to the Settings screen as shown.

Setting Method Security

The 67 Series spectrophotometers can support up to 10 individual users plus one Supervisor who has full administrative rights.

Logged in users can create methods with three levels of security options:

Personal – these methods are only accessible by the originator.

Public – these methods are available for use and modiﬁcation by any logged in user.

Read-Only – these methods can be accessed by all logged in users, but can only be modiﬁed by the

originator.

The preferred level of protection can be achieved by selecting the Method Security key that toggles between Personal, Public and Read-Only options.

To accept the selected options touch the Enter key to conﬁrm. The instrument will show the main measurement screen with the updated information.

To view the set parameters touch the status bar once and a drop down menu will appear. Touching this bar again will return it to its original status.

6715 CVS

Continue? , if so you will lose any changes you have made.

Yes No

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Setting Additional Set-up Parameters using the Toolbar Icons

Setting Analysis Points

Up to 30 analysis points can be set be selecting the Analysis Points keys and entering the required wavelength value via the numeric keypad.

The Clear Analysis Points option will clear all previously set analysis points.

To delete or edit individual analysis points select the required analysis point to display:

If Del is selected the analysis point is deleted.

If Edit is selected a new value can be entered via the numeric keypad.

If an attempt is made to set an analysis point outside the start and end wavelength values the error message ‘Wavelength for analysis out of spectrum scan range’ will be displayed momentarily and a triangular symbol will be shown to the side of the incorrect value. This value can be deleted or edited by selecting the analysis point (in this example 1) and entering a new value within the speciﬁed limits.

The analysis points will appear on tags at the relevant point on the spectrum display. They will also be printed with any printout to the internal or external printer if the Print Data Points option has been selected.

6715 CVS

What would you like to Do to this analysis point?

Del Edit

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Setting Auto-Scale

The Auto-Scale key toggles between On and Off. With the Auto-Scale function On the instrument will automatically set the Y-axis maxima to a level that will ﬁt the largest peak and lowest valley in the display area. This is the best option to choose if the user is uncertain of the limits of the scan.

When set to On the manual settings for Y-axis maximum and minimum are non-functional. These settings can be changed with Post Scan analysis tools to enable alternative views and printouts after the scan has been completed.

With the Auto-Scale function set to Off the Y-Axis Minimum enables the lowest displayed level for the Y-axis to be manually set. Select the Y-Axis Minimum key and a numeric keypad will be displayed for entry of this value. This value can be set between –0.301 and 2.999.

The Y-Axis Maximum enables the highest displayed level for the Y-axis to be manually set. Select the Y-Axis Maximum key and a numeric keypad will be displayed for entry of this value. This value can be set between 3.000 and –0.300.

Selecting Plot Colour

Selecting the Plot Colour key displays a colour selection screen. Touch any one of the eight colours to select the preferred plot colour. Once selected, the screen will update to show the selected colour.

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Selecting Axis Colour

Selecting the Axis Colour key displays a colour selection screen. Touch any one of the eight colours to select the preferred colour. Once selected, the screen will update to show the selected colour.

Setting Batch ID

Select the Batch ID key and enter the batch code using the alphanumeric keypad. Select Enter to accept or Cancel to remain as a default.

Setting Graph Data Points

The option to print graphs on a full page is only available if the External printer is selected. If available and set to On the scan will be printed on one page (typically A4 landscape format) with the data on the following pages.

If available and set to Off the scan will be printed with the data below it (typically top half A4 portrait).

The Print Graph Data Points key toggles between On and Off.

With this key set to On all the data points that make up the graph will be printed in a table following the scan.

Note: For a long scan at 0.1nm resolution this can result in many pages of data. It is recommended that careful consideration should be given to this and that this feature should be set to the Off position in this instance.

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Selecting Printer Media

The Printer Media options toggle between Internal and External Printers.

Auto Log Settings…

Selecting Auto Log Settings… opens a dedicated sub-menu. This option can be toggled between Off, On and Timed.

When the Auto Log option is selected to On the user can set the destination, (toggles between internal or external printer and memory).

When the Auto Log option is selected to Timed the user can set:

the timed interval between 3 and 9999 seconds using the numeric keypad;

the destination (toggles between internal or external printer and memory);

the number of repetitions from 2 to 999 using the numeric keypad.

To view the set parameters touch the status bar once and a drop down menu will appear. Touching this bar again will return it to its original status.

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File & Data Management

Save Tools Print Settings Erase Search Open Save Results Results

Saving Methods

Having entered all your required settings on the tabbed pages the method can be saved by simply pressing the Save key on the display surround. If you do not save at this point but continue to make measurements using the method, it will automatically be saved when you save the ﬁrst result.

If you continue without saving a result you will be prompted to save the method as you exit the operating mode or return to the settings options. Alternatively, when the Auto Log option is set to On or Timed and the destination option is Memory all results will be saved automatically.

Sharing Methods

Methods can be shared with other users by setting the security level at either Read-Only where other users can use but not modify the method or, Public where they can use and modify the method. Other users must then ensure that under Method View Settings (Settings/User Preferences/Method View Settings) they have enabled Public and/or Read-Only methods or turned All Methods on.

Recalling Methods

Following selection of the operating mode from the main menu the method browse screen is displayed. This will show all methods that the current user has access to, based on their selections in the Method View Settings (refer to Sharing Methods). If the ﬁrst page is full, cursor arrows will be displayed to enable navigation to and from additional pages.

Alternatively, selection may be made using the alphanumeric menu bar at the bottom of the screen.

Repeated pressing of each character set will display the full alphanumeric range and the screen will show all methods starting with the highlighted character.

Touch the required method when it is displayed on the screen to highlight it, touch the Open File icon to display the main measurement screen for that method.

Editing Methods

Use the Recalling Methods procedure to recall the required method. With the measurement screen displayed touch the Settings option. Adjust the settings as required and touch the Enter icon on completion. The modiﬁed method can then be saved by pressing the Save key on the display surround. If you do not save at this point but continue to make measurements using the method, it will automatically be saved when you save the ﬁrst result. If you continue without saving a result you will be prompted to save the method as you exit the operating mode or return to the settings options.

Note: If the Method name was not changed during editing it will be saved with the same name but with a new date and time to ensure traceability. If the old method is no longer required it should be deleted as detailed in Deleting Methods.

Deleting Methods

To delete methods highlight the required method in the Browse screen as described in Recalling Methods and then touch the Erase icon.

A warning message will be displayed to ensure this action is required. On conﬁrmation the selected ﬁle will be deleted.

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If the current user does not have the required privileges to delete the selected method then an information message will be displayed advising that the method cannot be deleted.

Privileges required for deleting designated methods:

Public Methods – only the Supervisor and Originator can delete these.

Read-Only Methods – only the Supervisor and Originator can delete these.

Personal Methods – Only the Originator can delete these methods. (The Supervisor can delete these

by re-setting the Originator’s PIN code and then logging in as the Originator).

Saving Results

After completion of a measurement the result can be saved by simply pressing the Save key on the display surround. The result is saved under the method that created it, with the entered Batch ID and an incremental number along with the time and date of the measurement. Results can also be saved as part of the Auto Log function by selecting Memory under the Auto Log option, which will vary depending on the type of sampling accessory ﬁtted.

Printing Results

After completion of a measurement the result can be printed, by simply pressing the Print key on the display surround. The result will be printed to either the internal or external printer, as selected by the user in the Printer Settings option.

The ﬁrst result of any new batch is preceded by a print header, which gives details of the method settings and Batch ID. Results can also be printed as part of the Auto Log function, which will vary depending on the type of sampling accessory ﬁtted.

Recalling Results

Stored results are always directly linked to the method that created them. To access results ﬁrst recall the method as described in Recalling Methods. With the method highlighted touch the Search Results icon. This will open a screen detailing all results available to the current user. Touch the required result or batch (depending on the mode) and then the Open Speciﬁc Result icon. This will display the results on the screen. The Tools option can then be used to work on these results (depending on mode). It is also possible to print the result by simply pressing the Print key on the display surround. Options to print to the Internal or External printer or to the CSV ﬁle will be displayed. Printing to the CSV (Comma Separated Values) ﬁle will save the data in CSV format on the external data card. (If a card is not ﬁtted the instrument will display a prompt). This is in text format and can be viewed and printed in Excel

®

.

In Kinetics mode an additional option is available (Analogue Mode). This allows results to be output via the rear panel analogue sockets to a chart recorder or similar device.

Sharing Results

Results attached to Personal Methods cannot be accessed by any other user.

Results attached to Read-Only and Public Methods can be accessed by all users, based on their current Method View Settings.

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6.4 PERFORMING MEASUREMENTS – all users

Performing a Scan

Once the required settings have been conﬁrmed the main Spectrum measurement screen will be shown with the Scan key inactive (blanked out):

Performing a Baseline

Insert a cuvette containing a blank solution into the sample holder and close the sample chamber lid. (Test tubes or other sample containers may be used depending on the sample holder accessory ﬁtted). Select the Baseline key. The messages ‘Performing baseline scan. Please wait…’ are displayed momentarily. Once the baseline measurement and storage is completed the screen will update to show:

Note: In general the blank solution should contain everything that is in the sample except the colour-producing component. For speciﬁc information reference should be made to the procedure or application being followed. For enhanced reproducibility matched cuvettes should be used.

Scanning the Sample

Once the baseline has been performed the Scan key will be active. Remove the cuvette containing the blank solution and insert the sample into the sample holder and close the sample chamber lid. Select the Scan key, if Auto Scale has been selected once the scan is completed, the screen will blank and the scan will be re-drawn to the optimum scaling.

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Additional individual samples can be measured by inserting them in the single sample holder and touching Scan. Alternatively, multiple samples can be measured with the optional 8 or 6 cell changers or sequentially with the sipper pump accessory.

Analysing the Scan

Selecting the icon gives access to post scan analysis options, as detailed below:

Note: All tools functions are available for use on recalled results in exactly the same manner as described hereafter.

Toolbar Icons

Display Settings

Toggles Auto Scale (Y Axis) On or Off. If Off Y axis defaults to manual settings entered in the method.

Cycles round Plot Interval settings of

0.1, 0.5, 1.0 and 5.0nm

Zoom

Activates the zoom area select cursors that can be positioned using the QWheel™ over the area required. Repeated presses increase the zoom up to 5 times.

Returns the zoom to the next lower level. At x1 this icon is disabled.

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Accepts and re-scales the selected area to the current zoom setting. Selecting the Back key or the Exit Door once maintains the zoom display and gives access to the tools for manipulation and analysis of the zoomed portion. A further press of the Back key returns the original scan.

Area Under Curve

Toolbar Icons

Area Under Curve Cursor Select Calculator

Selecting the Area Under Curve icon displays the area select cursors. This can be carried out on zoomed or recalled spectra as well as the current result. Initially both cursors are active.

Use the QWheel™ to position the right hand cursor at the right hand side of the curve to be analysed.

Press the Cursor Select icon to ﬁx this position, enabling the QWheel™ to move the left hand cursor to the other side of the required curve.

A further press of the Cursor Select icon ﬁxes this position but frees the right hand cursor for further ﬁne adjustment. (A further press enables both cursors again and so on round the cycle).

When the required area has been deﬁned selecting the Calculator icon displays the area under the curve, both as the displayed tangent (Tang.) to the valleys, and as a continuum to the X-axis (Base). The wavelengths at the start and end of the curve are also displayed.

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Co-ordinate Tagging

Toolbar Icons

Co-ordinate Tagging Tag Delete Tag

Peaks, valleys or any other points of interest can be selected (on the current, zoomed or recalled spectra) and have a co-ordinate tag added for future reference.

Selecting the Co-ordinate Tag icon enables the QWheel™ to control the displayed cursor. With the cursor on the required point pressing the Tag icon will add a tag at this point. All tags can be removed by selecting the Delete Tag icon.

Auto Peak & Valley Tagging

Toolbar Icons

Peak & Valley Calculate Find Peaks Display spectrum Scroll Up Scroll Down Tagging & Valleys Table with Peaks & Valleys

Peaks and valleys can be automatically identiﬁed on current, zoomed or recalled spectra. Selecting the Peak and Valley icon will cause the scan to be re-drawn with all peaks and valleys identiﬁed with co-ordinate tags. The initial display is with a setting of 20% (deﬁned as any turning point where the maximum or minimum value reached is 20% greater or less than the previous turning point). The sensitivity can be adjusted by selecting the Calculate icon and choosing the required sensitivity.

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Selecting the Find Peaks & Valleys Table icon displays the peaks and valleys in a table.

Scrolling through the table can be carried out using the Scroll Down and Scroll Up icons.

Selecting the Display Spectrum with Peaks and Valleys icon at any time returns to the spectrum display.

Obtaining Derivative Spectra

Toolbar Icon

Select Derivative

To obtain a derivative spectra select the Select Derivative icon and choose which derivative is required (the range is from None to 4

th

Derivative). The spectrum derivative display is updated to show

the spectrum.

Selecting the Back key returns to the original spectrum.

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Overlaying Scans

Toolbar Icon

Spectral Overlay Add Scans Information Display Display To Overlay More Icons Original Icons

Note: Scans can only be overlaid on Recalled Methods and the user has logged in, as access to stored data is required.

To overlay scans ﬁrst measure and store the scans for each sample required in the overlay, to a maximum of 8 scans.

Selecting the Spectral Overlay icon displays the overlay screen with the chosen recalled method displayed (the Spectral Overlay icon is displayed by selecting the Display More Icons icon from the Toolbar).

Selecting the Add Scans To Overlay icon opens a window detailing all available scans in the current batch.

Selecting the key detailing the required scan will re-draw the screen with the two scans overlaid. Further scans from the batch can be added in a similar way.

Selecting the Information icon opens a window enabling the colours of each displayed scan to be changed; by touching the colour swatch next to the details of each scan it will cycle through the 8 available alternatives. Similarly, any overlaid scan can be removed by touching the Delete icon alongside it.

Select the Back key to exit from either screen.

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Adding or Subtracting Spectra

Toolbar Icon

Add/Subtract Display Display Add Delete Return to Scans More Icons Original Icons Scans Scans Original Scan

Note: Scans can only be added or subtracted on Recalled Methods and the user has logged in, as access to stored data is required.

To add or subtract scans, ﬁrst measure and store the scans for each sample required.

Selecting the Add /Subtract icon displays the chosen recalled method displayed (the Add/Subtract icon is displayed by selecting the Display More Icons icon from the Toolbar).

Selecting either the Add Scans or Subtract Scans icon opens a window detailing all available scans in the current batch.

Selecting the key detailing the required scan will re-draw the screen with the two scans added or subtracted.

Selecting the Return to Original Scan icon updates the display to show the original method.

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SECTION 7 - Multi-Wavelength Mode

7.1 PRINCIPLES OF MEASUREMENT

The multi-wavelength mode is used to measure absorbance or transmittance at up to four distinct wavelengths. There are two ways in which this mode can then be used, one sample at four wavelengths or four samples each at a different wavelength when the eight position cell holder is ﬁtted. This mode is used for speciﬁc tests when one sample is examined and a ratio of absorbance values (or difference between absorbance values) at different wavelengths can reveal the purity or the composition of the sample. The ratio or difference calculations are automatically made by the spectrophotometer. The measurements are made almost simultaneously so the sample must be stable. Easily constructed methods for DNA or RNA purity/concentration can be implemented, with options for automatic or manual calibration and measurement.

Select the Multi-Wavelength Mode from the Main Menu options.

7.2 FREE OPERATION

Settings

If the user is not logged in then the main measurement screen will automatically be displayed when the Multi-Wavelength mode is selected from the Main Menu:

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To enter the required parameters for the sample(s) under test, select the Settings key and the instrument will display the following screen:

Mode settings – method name, measurement mode, sample style, method security (if logged in)

Setting wavelengths – allows 2 to 4 wavelengths to be set

Calculations - allows the selection of calculations and constants

Accessory options – varies with type of accessory module ﬁtted

Allows selection of internal or external printer, batch ID and the Auto Log options

Press to accept settings entered

Setting Method ID

To allocate a Method name select the Method key and enter the preferred name using the alphanumeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual letters or digits to be removed. The Cancel key will return you to the previous menu and the method name will remain as Default Method. The Enter key accepts the new method ID and returns you to the previous menu.

Note: The new Method ID can only be used to identify the sample or batch on a printout to the internal or external printer module as a single reading or a set of multiples if the Auto Log facility is used. The generic user has no additional facility to store or recall these results or the method settings.

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Selecting Measurement Mode

The Measurement Mode key toggles between Absorbance and Transmittance.

Selecting Sample Style

This option allows the user to select to measure one sample at up to four wavelengths, or to measure up to four samples at different wavelengths when the 8-position cell holder is ﬁtted.

Single – allows a single sample to be measured at up to 4 wavelengths.

Multiple – allows up to 4 samples to be measured at single different wavelengths.

This parameter is used in conjunction with setting wavelengths; i.e. in multiple option Sample 1 = Wavelength 1, Sample 2 = Wavelength 2, etc, with Position 0 reserved for a common blank.

Setting Method Security

This option is only applicable to logged in users.

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Setting Wavelengths

Up to four wavelengths can be set for each measurement parameter. The number of wavelengths to be measured can be set at 2, 3 or 4 by successive presses of the wavelength key. Values can be set by selecting the appropriate wavelength (#1-4) and entering the new value via the numeric keypad.

Note: The primary and secondary wavelengths can be selected using any combination e.g. Wavelength #1 as the primary and Wavelength #3 as the secondary.

Calculations

The calculations option allows the user to specify which calculations they would like to see performed with the readings taken at selected wavelengths and with speciﬁc samples.

Sum allows the user to specify which of the following sums is performed:

A1/A2 & A1-A2 ratio and difference will allow the user to see the ratio between any two readings – in this case the primary value divided by the secondary value, and the difference between two readings – the primary value minus the secondary value.

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(K1A1 + K2A2 + K3A3 + K4A4) x K5

K5 x (K1A1 + K2A2) / (K3A3 + K4A4)

Primary Wavelength allows the user to select which of the (up to) four wavelengths selected is used as the primary wavelength in the Ratio and Difference calculation.

Secondary Wavelength allows the user to select which of the (up to) four wavelengths selected is used as the secondary wavelength in the Ratio and Difference calculation. It is not possible to set the primary and secondary wavelengths to the same value.

K1 to K5 allow the user to set the required constants for the calculations.

Setting Batch ID

Select the Batch ID key and enter the batch code using the alphanumeric keypad. Select Enter to accept or Cancel to remain as a default.

Selecting Printer Media

The Printer Media key toggles between Internal and External Printer options.

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Auto Log Setting…

Selecting the Auto Log Settings… opens a dedicated sub-menu.

This option can be toggled between Off, On and Timed. When the Auto Log option is selected to On the user can set the destination (toggles between internal or external printer and memory);

When the Auto Log option is set to Timed the user can set:

the timed interval between 3 and 9999 seconds using the numeric keypad;

the destination (toggles between internal or external printer and memory);

the number of repetitions from 2 to 999 using the numeric keypad.

To view the set parameters touch the status bar once and a drop down menu will appear. Touching this bar again will return it to its original status.

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7.3 LOGGED IN MEASUREMENT

PIN Codes – each user is allocated a 4 digit PIN code that is required when logging in.

Select Log In… from the Main Menu and a list of users will be shown. Select the appropriate user name and a numeric keypad will be displayed.

Enter your 4 digit PIN code and touch the Enter key to conﬁrm.

Once logged in method screen options will be displayed:

Methods are stored sequentially by measurement mode. Once the ﬁrst page is full (8 methods for the selected mode) cursor arrows are displayed enabling the user to browse to subsequent or previous pages of 8 methods.

Alternatively, pressing one of the alphanumeric keys along the bottom of the screen will display all available methods with the initial character that is highlighted.

Note: Repeated pressing of a key sequentially highlights the characters between those displayed.

Create a New Method

For the following functions ﬁrst touch a method or result to select it.

Open the Selected Method

Erase the Selected Method

Browse Results – linked to the selected method

Open speciﬁc results in the selected batch

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Creating a New Method

Settings

To enter the Method ID, appropriate wavelength for the sample(s) to be tested and the level of Method Security required for the method being created select the New File icon

Setting Method ID

To allocate a Method name select the Method key and enter the preferred name using the alphanumeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual letters or digits to be removed. The Cancel key will return you to the previous menu and the method name will remain as Default Method. The Enter key accepts the new method ID and returns you to the previous menu.

Selecting Measurement Mode

The Measurement Mode key toggles between Absorbance and Transmittance.

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Selecting Sample Style

This option allows the user to select to measure one sample at up to four wavelengths, or to measure up to four samples at different wavelengths when the 8-position cell holder is ﬁtted. This parameter is used in conjunction with setting wavelengths; i.e. in multiple option Sample 1 = Wavelength 1, Sample 2 = Wavelength 2, etc, with position 0 reserved for a common blank.

Single – allows a single sample to be measured at up to 4 wavelengths.

Multiple – allows up to 4 samples to be measured at single different wavelengths.

Method Security

The 67 Series spectrophotometers can support up to 10 individual users plus one Supervisor who has full administrative rights.

Logged in users can create methods with three levels of security options:

Personal – these methods are only accessible by the originator.

Public – these methods are available for use and modiﬁcation by any logged in user.

Read-Only – these methods can be accessed by all logged in users, but can only be modiﬁed by the

originator.

The preferred level of protection can be achieved by selecting the Method Security key that toggles between Personal, Public and Read-Only options.

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Setting Wavelengths

Up to four wavelengths can be set for each measurement parameter. The number of wavelengths to be measured can be set at 2, 3 or 4 by successive presses of the wavelength key. Values can be set by selecting the appropriate wavelength (#1-4) and entering the new value via the numeric keypad.

Note: The primary and secondary wavelengths can be selected using any combination e.g. Wavelength #1 as the primary and Wavelength #3 as the secondary.

Calculations

The calculations option allows the user to specify which calculations they would like to see performed with the readings taken at selected wavelengths and with speciﬁc samples.

Sum allows the user to specify which of the following sums is performed:

A1/A2 & A1-A2 ratio and difference will allow the user to see the ratio between any two readings – in this case the primary value divided by the secondary value, and the difference between two readings – the primary value minus the secondary value.

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(K1A1 + K2A2 + K3A3 + K4A4) x K5

K5 x (K1A1 + K2A2) / (K3A3 + K4A4)

Primary Wavelength allows the user to select which of the (up to) four wavelengths selected is to be used as the primary wavelength in the Ratio and Difference calculation.

Secondary Wavelength allows the user to select which of the (up to) four wavelengths selected is to be used as the secondary wavelength in the Ratio and Difference calculation. It is not possible to set the primary and secondary wavelengths to the same value.

K1 to K5 allow the user to set the required constants for the calculations.

Setting Batch ID

Select the Batch ID key and enter the batch code using the alphanumeric keypad. Touch Enter to accept or Cancel to remain as a Default.

Selecting Printer Media

The Printer Media key toggles between Internal and External Printer options.

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Auto Log Settings…

Selecting the Auto Log Settings… opens a dedicated sub-menu.

This option can be toggled between Off, On and Timed .

When the Auto Log option is selected to On the user can set the destination (toggles between internal or external printer and memory);

When the Auto Log option is set to Timed the user can set:

the timed interval between 3 and 9999 seconds using the numeric keypad;

the destination (toggles between internal or external printer and memory);

the number of repetitions from 2 to 999 using the numeric keypad.

To view the set parameters touch the status bar once and a drop down menu will appear. Touching this bar again will return it to its original status.

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File & Data Management

Save Tools Print Settings Erase Search Open Save Results Results

Saving Methods

Having entered all your required settings on the tabbed pages the method can be saved by simply pressing the Save key on the display surround. If you do not save at this point but continue to make measurements using the method, it will automatically be saved when you save the ﬁrst result.

If you continue without saving a result you will be prompted to save the method as you exit the operating mode or return to the settings options. Alternatively, when the Auto Log option is set to On or Timed and the destination option is Memory all results will be saved automatically.

Sharing Methods

Methods can be shared with other users by setting the security level at either Read-Only where other users can use but not modify the method or, Public where they can use and modify the method. Other users must then ensure that under Method View Settings (Settings/User Preferences/Method View Settings) they have enabled Public and/or Read-Only methods or turned All Methods on.

Recalling Methods

Following selection of the operating mode from the main menu the method browse screen is displayed. This will show all methods that the current user has access to, based on their selections in the Method View Settings (refer to Sharing Methods). If the ﬁrst page is full, cursor arrows will be displayed to enable navigation to and from additional pages.

Alternatively, selection may be made using the alphanumeric menu bar at the bottom of the screen.

Repeated pressing of each character set will display the full alphanumeric range and the screen will show all methods starting with the highlighted character.

Touch the required method when it is displayed on the screen to highlight it, touch the Open File icon to display the main measurement screen for that method.

Editing Methods

Use the Recalling Methods procedure to recall the required method. With the measurement screen displayed touch the Settings option. Adjust the settings as required and touch the Enter icon on completion. The modiﬁed method can then be saved by pressing the Save key on the display surround. If you do not save at this point but continue to make measurements using the method, it will automatically be saved when you save the ﬁrst result. If you continue without saving a result you will be prompted to save the method as you exit the operating mode or return to the settings options.

Note: If the Method name was not changed during editing it will be saved with the same name but with a new date and time to ensure traceability. If the old method is no longer required it should be deleted as detailed in Deleting Methods.

Deleting Methods

To delete methods highlight the required method in the Browse screen as described in Recalling Methods and then touch the Erase icon.

A warning message will be displayed to ensure this action is required. On conﬁrmation the selected ﬁle will be deleted.

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If the current user does not have the required privileges to delete the selected method then an information message will be displayed advising that the method cannot be deleted.

Privileges required for deleting designated methods:

Public Methods – only the Supervisor and Originator can delete these.

Read-Only Methods – only the Supervisor and Originator can delete these.

Personal Methods – Only the Originator can delete these methods. (The Supervisor can delete these

by re-setting the Originator’s PIN code and then logging in as the Originator).

Saving Results

After completion of a measurement the result can be saved by simply pressing the Save key on the display surround. The result is saved under the method that created it, with the entered Batch ID and an incremental number along with the time and date of the measurement. Results can also be saved as part of the Auto Log function by selecting Memory under Auto Log options, which will vary depending on the type of sampling accessory ﬁtted.

Printing Results

After completion of a measurement the result can be printed, by simply pressing the Print key on the display surround. The result will be printed to either the internal or external printer, as selected by the user in the Printer Settings option.

The ﬁrst result of any new batch is preceded by a print header, which gives details of the method settings and Batch ID. Results can also be printed as part of the Auto Log function, which will vary depending on the type of sampling accessory ﬁtted.

Recalling Results

Stored results are always directly linked to the method that created them. To access results ﬁrst recall the method as described in Recalling Methods. With the method highlighted touch the Search Results icon. This will open a screen detailing all results available to the current user. Touch the required result or batch (depending on the mode) and then the Open Speciﬁc Result icon. This will display the results on the screen. The Tools option can then be used to work on these results (depending on mode). It is also possible to print the result by simply pressing the Print key on the display surround. Options to print to the Internal or External printer or to the CSV ﬁle will be displayed. Printing to the CSV (Comma Separated Values) ﬁle will save the data in CSV format on the external data card. (If a card is not ﬁtted the instrument will display a prompt). This is in text format and can be viewed and printed in Excel

®

.

In Kinetics mode an additional option is available (Analogue Mode). This allows results to be output via the rear panel analogue sockets to a chart recorder or similar device.

Sharing Results

Results attached to Personal Methods cannot be accessed by any other user.

Results attached to Read-Only and Public Methods can be accessed by all users, based on their current Method View Settings.

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7.4 PERFORMING MEASUREMENTS – all users

Place the zero solution in the sample chamber and close the lid.

Selecting the Zero key will start the cycle, setting zero at each wavelength in turn. A series of information boxes will advise of the progress.

When completed all displayed absorbance values will be set to zero (or 100% Transmittance).

Replace the zero solution with the sample to be measured and close the sample chamber lid.

Selecting the Read key will start the measure cycle, measuring at each wavelength in turn. A series of information boxes will advise of the progress.

When completed the measured absorbances will be displayed.

The results of the selected calculation will be displayed below the Wavelength/Absorbance data.

Saving a method allows access to the table through Recalling a Method.

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SECTION 8 - Kinetics Mode

8.1 PRINCIPLES OF MEASUREMENT

A number of tests run on a spectrophotometer involve an active ingredient. On addition of this active ingredient, the absorbance or the transmittance of the sample, will be seen to either increase or decrease. The absorbance or transmittance is recorded at regular intervals at a preset wavelength for a set time.

A graph is then plotted to show how the absorbance or transmittance changes over time. From the graph, the concentration of the sample can be calculated. This mode is ideal for continuous monitoring as well as the calculation of concentration in enzyme activity studies, with calibration possible against a standard or known factor.

Select the Kinetics Mode from the Main Menu options:

8.3 FREE OPERATION

Settings

If the user is not logged in then the main measurement screen will automatically be displayed when the Kinetics mode is selected from the Main Menu.

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To enter the required parameters for the sample(s) under test, select the Settings key and the instrument will display the following screen:

Mode settings - method name, wavelength, measurement mode, run settings, method security (if logged in)

Calibration – allows setting of Concentration cal standard, factor, resolution and units of measurement

Measurement display – allows auto scaling, axis setting and colour selection

Accessory options – varies with type of accessory module ﬁtted

Allows selection of internal or external printer, graph details, batch ID and the Auto Log options

Press to accept settings entered

Setting Method ID

To allocate a Method name select the Method key and enter the preferred name using the alphanumeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual letters or digits to be removed. The Cancel key will return you to the previous menu and the method name will remain as Default Method. The Enter key accepts the new method ID and returns you to the previous menu.

Note: The new Method ID can only be used to identify the method on a printout to the internal or external printer module. The generic user has no additional facility to store or recall these results or the method settings.

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Setting Wavelength

Select the Wavelength key and enter the wavelength using the numeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual digits to be removed. The Cancel key will return you to the previous menu and the wavelength will not alter. The Enter key accepts the new wavelength and returns you to the previous menu. (At certain wavelengths order selecting ﬁlters may be heard switching in or out).

Selecting Measurement Mode

The Measurement Mode key toggles between Absorbance and Transmittance.

Run Settings…

Selecting Run Settings… opens a dedicated sub-menu.

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Setting Run Time

Selecting the Run Time key displays a numeric keypad. Clear the current setting and enter the required time, in seconds, for the measurement period. The limits for this setting are from 30 to 9999 seconds (2.75 hours).

Setting Lag Time

Selecting the Lag Time key displays a numeric keypad. Clear the current setting and enter the required lag time in seconds. The lag time is a portion of the run time over which no data is collected, starting from the beginning of the run time. This allows for any latency or non-linear reaction to be disregarded. Setting this to zero means data will be collected over the full run time. The limits for this setting are from 0 to 9999 seconds.

Setting Start On Level

Selecting the Start On Level key disables the lag time option. Repeated presses of this key cycle through the Greater Than, Less Than and Disabled options. With Greater Than or Less Than selected data collection will start when the measured value (Absorbance or Transmittance) is above or below the value entered in the Start Level setting.

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Setting Start Level

Selecting the Start Level key displays a numeric keypad. Clear the current setting and enter the required level above or below which data collection is required.

Limits for this setting are – 0.299 to 2.999Abs and 0.1 to 199.1%T.

Setting Method Security - This option is only applicable to logged in users.

Setting Concentration Parameters

The rate of change of absorbance can be converted to concentration using a known factor or reference concentration value. Whichever is not required should be set to unity so that it is ignored in the calculation.

Standard

Select the Standard key and enter the concentration value of the calibration standard. Values from

–9999.99 to 9999.99 can be entered. If a concentration value is not to be used then this should be set to 1.00.

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Factor

This is the value by which the rate of change is multiplied to give the concentration. Select the Factor key and enter the factor value. Values from –9999.99 to 9999.99 can be entered. If a Factor is not to be used this value should be set to 1.00.

Selecting Resolution

This enables the resolution of the result to be set. Resolution can be set using the Resolution key to scroll through the available options.

Resolution options are: 1, 0.1, 0.01 and 0.001.

Selecting Units

Selection of units is made using the Units key to scroll through the available measurement options. The following units are available:- mEq/l, ppm, mg/l, g/l, %, µg/ml, mg/ml, g/dl, mg/dl, µg/l, ng/l, µg/dl, M, mM, µM/l, U/l, mU/l, U/ml, blank.

Note: The unit is simply a name tag added to the result. Due to the variety of samples that may be measured it is impossible to enable any direct conversion between units.

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Setting Auto-Scale

The Auto-Scale key toggles between On and Off. With the Auto-Scale function On the instrument will automatically set the Y-axis maxima to a level that will ﬁt the graph of the kinetics run. When set to Off the manual settings for Y-axis maximum and minimum are non-functional. These settings can be changed with post run analysis tools to enable alternative views and printouts after the run has been completed.

With the Auto-Scale function set to Off the Y-Axis Minimum enables the lowest displayed level for the Y-axis to be manually set. Select the Y-Axis Minimum key and a numeric keypad will be displayed for entry of this value. This value can be between –0.301 and 2.999.

The Y-Axis Maximum enables the highest displayed level for the Y-axis to be manually set. Select the Y-Axis Maximum key and a numeric keypad will be displayed for entry of this value. This value can be between 3.000 and –0.300.

Selecting Axis Colour

Selecting the Axis Colour key displays a colour selection screen. Touch any one of the eight colours to select the preferred colour. Once selected, the screen will update to show the selected colour.

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Setting Batch ID

Select the Batch ID key and enter the batch code using the alphanumeric keypad. Select Enter to accept or Cancel to remain as a default.

Setting Graph Data Points

The option to print graphs on a full page is only available if the External printer is selected. If available and set to On the graph will be printed on one page (typically A4 landscape format) with the data on following pages.

If available and set to Off the graph will be printed with the data below it (typically top half A4 portrait).

The Print Graph Data Points key toggles between On and Off.

With this key set to On all the data points that make up the graph will be printed in a table following the graph.

Selecting Printer Media

The Printer Media options toggle between Analog, Internal and External Printers.

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Auto Log Settings…

Selecting Auto Log Settings… opens a dedicated sub-menu.

This option can be toggled between Off, On and Timed.

When the Auto Log option is selected to On the user can set the destination (toggles between internal and external printer, analog and memory);

When the Auto Log option is set to Timed the user can set;

timed interval between 3 and 9999 seconds using the numeric keypad;

the destination (toggles between internal or external printer and memory);

the number of repetitions from 2 to 999 using the numeric keypad.

To view the set parameters touch the status bar once and a drop down menu will appear. Touching this bar again will return it to its original status.

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8.3 LOGGED IN MEASUREMENT

PIN Codes – each user is allocated a 4 digit PIN code that is required when logging in.

Select Log In… from the Main Menu and a list of users will be shown. Select the appropriate user name and a numeric keypad will be displayed.

Enter your 4 digit PIN code and press the Enter key to conﬁrm.

Once logged in method screen options will be displayed:

Methods are stored sequentially by measurement mode. Once the ﬁrst page is full (8 methods for the selected mode) cursor arrows are displayed in the top corners enabling the user to browse to subsequent or previous pages of 8 methods.

Alternatively, pressing one of the alphanumeric keys along the bottom of the screen will display all available methods with the initial character that is highlighted.

Note: Repeated pressing of a key sequentially highlights the characters between those displayed.

Create a New Method

For the following functions ﬁrst touch a method or result to select it.

Open the Selected Method

Erase the Selected Method

Browse Results – linked to the selected method

Open speciﬁc results in the selected batch

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Creating a New Method

To enter the Method ID, appropriate measurement parameters and the level of Method Security required for the method being created select the New File icon.

Setting Method ID

To allocate a Method name select the Method key and enter the preferred name using the alphanumeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual letters or digits to be removed. The Cancel key will return you to the previous menu and the method name will remain as Default Method. The Enter key accepts the new method ID and returns you to the previous menu.

Setting Wavelength

Select the Wavelength key and enter the wavelength using the numeric keypad. The Clear key will clear all information entered from the screen. The Del key allows individual digits to be removed. The Cancel key will return you to the previous menu and the wavelength will not alter. The Enter key accepts the new wavelength and returns you to the previous menu. (At certain wavelengths order selecting ﬁlters may be heard switching in or out).

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Selecting Measurement Mode

The Measurement Mode key toggles between Absorbance and Transmittance.

Run Settings…

Selecting Run Settings… opens a dedicated sub-menu.

Setting Run Time

Selecting the Run Time key displays a numeric keypad. Clear the current setting and enter the required time, in seconds, for the measurement period. The limits for this setting are from 30 to 9999 seconds (2.75 hours).

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Setting Lag Time

Selecting the Lag Time key displays a numeric keypad. Clear the current setting and enter the required lag time in seconds. The lag time is a portion of the run time over which no data is collected, starting from the beginning of the run time. This allows for any latency or non-linear reaction to be disregarded. Setting this to zero means data will be collected over the full run time. The limits for this setting are from 0 to 9999 seconds.

Setting Start On Level

Selecting the Start On Level key disables the lag time option. Repeated presses of this key cycle through the Greater Than, Less Than and Disabled options. With Greater Than or Less Than selected data collection will start when the measured value (Absorbance or Transmittance) is above or below the value entered in the Start Level setting.

Setting Start Level

Selecting the Start Level key displays a numeric keypad. Clear the current setting and enter the required level above or below which data collection is required.

Limits for this setting are – 0.299 to 2.999Abs and 0.1 to 199.1%T.

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Setting Method Security

The 67 Series spectrophotometers can support up to 10 individual users plus one Supervisor who has full administrative rights.

Logged in users can create methods with three levels of security options:

Personal – these methods are only accessible by the originator.

Public – these methods are available for use and modiﬁcation by any logged in user.

Read-Only – these methods can be accessed by all logged in users, but can only be modiﬁed by the

originator.

The preferred level of protection can be achieved by selecting the Method Security key that toggles between Personal, Public and Read-Only options.

Setting Concentration Parameters

The rate of change of absorbance can be converted to concentration using a known factor or reference concentration value. Whichever is not required should be set to unity so that it is ignored in the calculation.

Standard

Select the Standard key and enter the concentration value of the calibration standard. Values from –9999.99 to 9999.99 can be entered. If a concentration value is not to be used then this should be set to 1.00.

Jenway 6715, 6705, 6700 Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual