Eppendorf Top Buret M- H User Manual
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Top Buret
Operating manual
™
Copyright
©
2013 Eppendorf AG, Hamburg. No part of this publication may be
reproduced without the prior permission of the copyright owner.
Eppendorf and the Eppendorf logo are a registered trademark of Eppendorf AG.
®
Trademarks are not marked in all cases with ™ or
in this manual.
4965 900.018-03/032013
Table of contents
Top Bu ret
English (EN)
Operating manual Top Buret™seeon p.Fig.Tab.p.
English (EN)Operating manual
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
2.4 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
3.3 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
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4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 Mounting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
4.2 Inserting the batteries. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.3 Mounting the telescopic aspirating tube . . . . . . . . . . . . . . . . . . . . . 18
4.3.1 Extending the telescopic aspirating tube . . . . . . . . . . . . . . 18
4.3.2 Shortening the telescopic aspirating tube . . . . . . . . . . . . . 18
4.3.3 Mounting the telescopic aspirating tube . . . . . . . . . . . . . .19
4.4 Mounting the Top Buret on the bottle . . . . . . . . . . . . . . . . . . . . . . . 19
4.5 Connecting the drying tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.1 Dispensing settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.2 Deairing the Top Buret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
5.3 Titrating liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24
5.4 Changing the supply bottles during titration . . . . . . . . . . . . . . . . . .25
5.5 Removing the bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26
5.6 Emptying the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26
Table of contents
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Top Buret
English (EN)
™
6 Calibration and adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
6.1 Calibrating the Top Buret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.1.1 Measuring the testing volume . . . . . . . . . . . . . . . . . . . . . . 27
6.1.2 Calculating the error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.1.3 Evaluating the calibration . . . . . . . . . . . . . . . . . . . . . . . . . .28
6.2 Adjusting the Top Buret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
6.2.1 Adjustment to distilled water . . . . . . . . . . . . . . . . . . . . . . .29
6.2.2 Adjustment to a liquid with a different density. . . . . . . . . . 29
6.3 Resetting the Top Buret to the factory settings . . . . . . . . . . . . . . . .30
7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.1 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
8 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32
8.1 Cleaning the Top Buret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32
8.1.1 Standard cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
8.1.2 Intensive cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8.2 Disassembling the Top Buret . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
8.3 Replacing the batteries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
9 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
9.1 Ambient conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
9.2 Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
9.3 Parameters for titratable media . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
9.4 Conditions for calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
10 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
10.1 Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
10.2 Spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
10.3 Threaded adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
11 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
11.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
11.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
11.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
12 Measurement report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Operating instructions
Top Bu ret
English (EN)
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first
time. Please also note the operating instructions for the accessories, if
applicable.
This operating manual is part of the product. Thus, it must always be easily
accessible.
Enclose this operating manual when transferring the device to third parties.
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols
and danger levels:
1.2.1 Danger symbols
Biohazard Explosion
Tox ic subst ances Hazard point
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Material damage
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WARN ING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
Operating instructions
6
Top Buret
English (EN)
™
1.3 Symbols used
Symbol Meaning
Handling
1.
Actions in the specified order
2.
• List
Text Name of fields in the software
Useful information
1.4 Abbreviations used
ETFE
Ethylene-tetrafluoroethylene copolymer
FEP
Tetrafluoroethylene-perfluoropropylene copolymer
FKM
Fluororubber
PFA
Perfluoroalkoxy copolymer
PP
Polypropylene
PTFE
Polytetrafluorethylene
PVDF
Polyvinylidene fluoride
2 Product description
Start
Pause
ml
Calibrate
Reset
Top Buret M
1
5
4
3
2
8
7
6
2.1 Main illustration
Abb. 2-1: Top Buret M/H
Product description
Top Bu ret
English (EN)
™
7
Fig. 2-1: Top Buret M/H
1 Control panel
2 Discharge tube
3 Canula holder
4 Canula arm
5 Discharge valve toggle
6 Telescopic aspirating tube
7 Bottle thread
8 Dispensing wheels
Product description
Top Buret H
1
2
3
7
8
9
6
5
4
8
Top Buret
English (EN)
Abb. 2-2: Operating controls
™
Fig. 2-2: Operating controls
1Calibration mode
2Volume display
3Arrow keys
4 Opening symbol
5Start key
6Pause key
7 Battery flat symbol
8 Titration mode symbol
9 Pause symbol
2.2 Delivery package
Number Description
1 Eppendorf Top Buret M 25 mL
or Eppendorf Top Buret H 50 mL
1 Canula arm
1 Discharge tube with knurled nut
1 Canula holder
1 Telescopic aspirating tube
1 Bottle thread adapter
From GL 45 to S 40 (buttress thread), PP
From GL 45 to GL 38, PP
From GL 38 to GL 32, PP
1 Battery
LR03/AAA, 1.5 V, set of 2
1 Eppendorf Top Buret M/H operating manual
2.3 Features
Product description
Top Bu ret
English (EN)
™
9
The Top Buret is a bottle-top buret which features a continuous, pulse-free
dispensing technique. The Top Buret can dispense volumes between 10 μL and
999.9 mL at a maximum concentration of 1 mol/L.
When the dispensing wheels are fully rotated, the following volumes are
dispensed:
• Top Buret M: 2.5 mL
• Top Buret H: 5.0 mL
The Top Buret has two dispensing settings. The dispensing settings are set with
the toggle on the canula arm.
• Titration
Titrate liquid from the bottle to a destination vessel.
• Deairing
Rinse liquid through the discharge valve into the bottle, e.g., to remove air
bubbles.
Product description
10
Top Buret
English (EN)
™
2.4 Materials
NOTICE! Aggressive substances may damage the Top Buret and
accessories.
Check the chemical resistance before using organic solvents or
aggressive chemicals.
Follow the cleaning instructions.
The assembly parts of the Top Buret consist of the following materials:
Assembly parts that come into
contact with liquid
• Borosilicate glass 3.3 (Boro 3.3)
• Ethylene-tetrafluoroethylene
copolymer (ETFE)
• Perfluoroalkoxy copolymer (PFA)
• Tetrafluoroethylene-
perfluoropropylene copolymer (FEP)
• Platinum iridium (Pt-Ir)
• Polytetraflourethylene (PTFE)
Assembly parts that do not come into
contact with liquid
• Stainless steel
• Fluororubber (FKM)
•Polyester
•Polypropylene (PP)
• Polyvinylidene fluoride (PVDF)
• Polyamide (PA)
• Polyoxymethylene (POM)
• Polyphenylene sulfide (PPS)
Safety
Top Bu ret
English (EN)
3 Safety
3.1 Intended use
The Top Buret is a laboratory device for dispensing aqueous solutions in a
volume range between 10 μL and 999.9 mL. Applications in or on the human
body (in vivo applications) are not permitted.
The device must not be exposed to an aggressive atmosphere, e.g., HCI vapors.
The device must not be autoclaved.
The device is intended for dispensing liquids with the following properties:
• Maximum concentration: 1 mol/L
• Maximum density: 2.2 g/cm
• Maximum vapor pressure: 50 kPa
The following reagents must not be titrated with the device:
• Liquids which attack PFA, ETFE, PTFE, Boro 3.3 or FEP
• Solutions containing hydrofluoric acid, since they attack borosilicate glass
• Unstable substances because they react catalytically with platiniridium, e.g.,
hydrogen peroxide
• Solutions prone to crystallization
• Suspensions containing solid particles
• Fuming acids or highly concentrated alkaline solutions
• Solutions which decompose and form solid particles, e.g., Biuret reagent
• Carbon disulfide, as it is highly flammable
3
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The Top Buret is suitable for dispensing the following titration solutions up
to a concentration of 1 mol/L:
• Ammonium iron(II) sulfate solution
• Ammonium thiocyanate solution
• Barium chloride solution
• Bromid bromate solution
• Cerium(IV) sulfate solution
• EDTA solution
• Iron(II) sulfate solution
• Acetic acid
Safety
12
Top Buret
English (EN)
™
• Iodine solution
• Caustic potash solution
• Potassium bromate solution
• Potassium bromide bromate solution
• Potassium dichromate solution
• Potassium iodate solution
• Potassium permanganate solution
• Potassium thiocyanate solution
• Sodium arsenite solution
• Sodium carbonate solution
• Sodium chloride solution
• Sodium nitrite solution
• Sodium thiosulfate solution
• Caustic soda
•Oxalic acid
•Perchloric acid
• Nitric acid
•Hydrochloric acid
•Sulfuric acid
•Silver nitrate solution
• Tetra-n-butylammonium hydroxide solution
• Zinc sulfate solution
Refer to the information provided by the reagent manufacturers. If you have any
questions, contact Eppendorf Service.
3.2 User profile
The device and accessories may only be operated by trained and skilled
personnel.
Before using the device, read the operating manual carefully and familiarize
yourself with the device's mode of operation.
3.3 Warnings for intended use
DANGER! Risk of explosion.
Do not operate the device in areas where work is completed with
explosive substances.
Do not use this device to process any explosive or highly reactive
substances.
Do not use this device for processing any substances which could
generate an explosive atmosphere.
WARNING! Damages to health due to infectious liquids and
pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the
national regulations, the biological security level of your laboratory,
the material safety data sheets, and the manufacturer's application
notes.
Wear personal protective equipment.
Consult the "Laboratory Biosafety Manual" (Source: World Health
Organization, Laboratory Biosafety Manual, as amended) for
comprehensive regulations on the handling of risk group II germs or
biological materials).
Safety
Top Bu ret
English (EN)
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13
WARNING! Damage to health due to toxic, radioactive or aggressive
chemicals.
Wear personal protective equipment.
Observe the national regulations for handling these substances.
Observe the material safety data sheets and manufacturer's
application notes.
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