Eppendorf 5418 R, 5418 User Manual

5401 900.022-00/0909

Centrifuge 5418/5418 R

Operating manual

Copyright

©

2009 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of

the copyright owner.

Trademarks

eppendorf® is a registered trademark of Eppendorf AG, Hamburg, Germany.
Microtainer® is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.

Trademarks are not marked in all cases with ™ or

®

in this manual.

5401 900.022-02/0710

You will find a detailed description of these figures in your language in Chapters 2.1 and 4.1/5.1.

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Abb. 1: Front and rear view of the Ce ntrifuge 5418

Fig. 1: Front and rear view of the Centrifuge 5418

Abb. 2: Front and rear view of the Ce ntrifuge 5418 R

Fig. 2: Front and rear view of the Centrifuge 5418 R

1 Centrifuge lid 2 Monitoring glass

3 Display 4 Control panel

5 Emergency lid release 6 ID plate

7 Mains switch 8 Mains connection

9 Fuse holder 10 Condensation water tray

Abb. 3: Control panel of the Centr ifuge 5418 and Ce ntrifuge 5418 R.

Fig. 3: Control panel of the Centrifuge 5418 and Centrifuge 5418 R.

1 Switch the displayed centrifuging speed (rpm/rcf) 2 Display

3 Release lid 4 Adjust the centrifuging duration

Only 5418 R: Press the time dial to start and stop

centrifugation.

5 Set the speed of centrifugation

6 Adjust the temperature (only 5418 R)

Only 5418 R: Press the speed dial to switch the

displayed centrifugation speed (rpm/rcf).

7 Start the temperature control run fast temp 

8 Short spin centrifugation

(only 5418 R)

9 Start and stop centrifugation

Abb. 4: Display of the Centrifug e 5418 and Centrifug e 5418 R.

Fig. 4: Display of the Centrifuge 5418 and Centrifuge 5418 R.

1 Centrifuging duration 2 g-force (rcf) or speed (rpm)

3 Status of the centrifuge 4 At set rpm (only 5418 R)

5 Temperature (only 5418 R) 6 Status of the key lock (only 5418 R)

7 Status of the centrifugation speed display 8 Status of the loudspeaker (only 5418 R)

Table of contents

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

Operating manual

Table of contents

1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Operating manual

1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2 Warning signs and hazard icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.3 Symbols used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.4 Abbreviations used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2.1 Centrifuge 5418 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2.2 Centrifuge 5418 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.4 Rotor FA-45-18-11 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.4.1 Rcf display and calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3 Application limits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3.1 Declaration concerning the ATEX directive (94/9/EC). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.5.2 Incorrect handling of the centrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.5.4 Extreme strain on the centrifuging tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.5.5 Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.6 Safety instructions on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

4.2 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.1 Overview of operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.2 Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.2.1 Switching on the centrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.2.2 Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.2.3 Loading the rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5.2.4 Closing the rotor lid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.2.5 Closing the centrifuge lid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.3 Cooling (only 5418 R). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.3.1 Temperature adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.3.2 Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.3.3 Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.3.4 Fast Temp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.3.5 Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.4 Centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.4.1 Centrifuging with time preset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.4.2 Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5.4.3 Short spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.4.4 Removing the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.5 Standby mode (only 5418) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.6 Special functions (only 5418 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

EN

Operating manual

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EN

Operating manual

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

Table of contents

6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.1 Prepare cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.2 Perform cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.2.1 Clean/disinfect device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.2.2 Clean/disinfect rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.3 Additional service instructions for Centrifuge 5418 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

6.4 Glass breakage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

6.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

6.6 Decontamination before shipping. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

7.3 Emergency lid release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.3.1 Centrifuge 5418 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.3.2 Centrifuge 5418 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

8.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

8.2 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

8.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

9.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

9.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

9.3 Weight / dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

9.4 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

10 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.1 Centrifuge 5418 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.2 Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.3 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.4 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.4.1 Fuses for Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10.4.2 Fuses for Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6

Centrifuge 5418 / 5418 R — Operating manual

1 User instructions

1 User instructions

1.1 Using this manual

1.2 Warning signs and hazard icons

1.2.1 Hazard symbols

Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

Please read this operating manual completely before using the device for the first time.


 Please view this operating manual as part of the product and keep it somewhere easily

accessible.

 When passing the device on to third parties, be sure to include this operating manual.
 If this manual is lost, please request another one. The current version can be found on our

website www.eppendorf.com.

Biological hazard Danger of explosion

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EN

Operating manual

Danger of electric shock Danger of crushing

General danger Material damage

1.2.2 Degrees of danger

The degree of danger is a part of a safety note and distinguishes the possible results of
non-observance from each other.

DANGER Will lead to severe injuries or death.
WARNING Can lead to severe injuries or death.
CAUTION Can lead to light to moderate injuries.
NOTICE Can lead to material damages.

1.3 Symbols used

Depiction Meaning

 You are requested to perform an action.

1.

2.

Perform these actions in the sequence described.

• List.

Text Terms and key names from the software.

References useful information.

1.4 Abbreviations used

PCR Polymerase chain reaction

rcf Relative centrifugal force

rpm Revolutions per minute

UV Ultraviolet radiation

7

1

3
4

2

1

6

7
8
9

2

5

1

3

4

10

2

1

5

6

9

7

10

8

2

EN

Operating manual

Centrifuge 5418 / 5418 R — Operating manual

2 Product description

2 Product description

2.1 Main illustration

Abb. 1: Front and rear view of the Ce ntrifuge 5418

Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

The depiction of the Centrifuge 5418 / 5418 R can also be found on the front fold-out page 
(see Fig. 1 + 2).

finale Korrektur

Fig. 1: Front and rear view of the Centrifuge 5418

Abb. 2: Front and rear view of the Ce ntrifuge 5418 R

Fig. 2: Front and rear view of the Centrifuge 5418 R

1 Centrifuge lid 2 Monitoring glass

Visual control for rotor stop or option for speed control
via stroboscope.

3Display

Depiction of the current centrifuging parameters and

4 Control panel

For operating the centrifuge (see Fig. 3 on p. 17).

device settings (see Fig. 4 on p. 17).

5 Emergency lid release

(see Emergency lid release on p. 29)

7 Mains switch

Switch for switching the device on and off.
Switch position 0: The device is switched off.
Switch position I: The device is switched on.

9 Fuse holder 10 Condensation water tray (only 5418 R)

6ID plate

8 Mains connection

Connection socket for the mains cable supplied.

8

Centrifuge 5418 / 5418 R — Operating manual

2 Product description

2.2 Delivery package

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EN

2.2.1 Centrifuge 5418

Quantity Order No.

(International)

1- -

1
or

1 5416 301.001 022634305

1 5703 350.102 022639609
1- - Mains cable

1
1

2.2.2 Centrifuge 5418 R

Quantity Order No.

1- -

1
or

1 5416 301.001 022634305
1 5401 850.076 5401850076 Tray for condensation water
1- - Mains cable

1
1

5425 351.003
5425 353.006

5401 900.022
5401 900.030

(International)

5425 351.003
5426 355.100

5401 900.022
5401 900.030

Order No.
(North America)

022668188
022668226

5401900022
5401900030

Order No.
(North America)

022668188
022668200

5401900022
5401900030

Description

Centrifuge 5418

See the Ordering Information chapter for corresponding device
version, equipment and order number

Fuses

2.5 AT (230 V), 2 pieces
5 AT UL (100 V/120 V), 2 pieces

Rotor key

Standard

Captain Eppi rotor key holder

1 piece

Operating manual Centrifuge 5418/5418 R

Languages: EN, DE, FR, ES, IT, PT
Languages: DA, FI, EL, NL, SV (230 V devices only)

Description

Centrifuge 5418 R

See the Ordering Information chapter for corresponding device
version, equipment and order number

Fuses

2.5 AT (230 V), 2 pieces

6.25 AT (100 V/120 V), 2 pieces

Rotor key

Standard

Operating manual Centrifuge 5418/5418 R

Languages: EN, DE, FR, ES, IT, PT
Languages: DA, FI, EL, NL, SV (230 V devices only)

Operating manual

2.3 Features

The compact and easy-to-use Centrifuge 5418 / 5418 R has a capacity of 18 x 2 mL and reaches
a maximum of 16,873 x g / 14,000 rpm. The microcentrifuge is equipped with an aerosol-tight
standard rotor for centrifugation of the following tubes:

• Micro test tubes (0.2 to 2.0 mL)

• Microtainers (0.6 mL)

• Spin columns (1.5/2.0 mL)

The Centrifuge 5418 R has an additional temperature control function for centrifugation between
0°C and +40°C. The fast temp function can be used to start a temperature control run without
samples to adjust the rotor chamber incl. rotor and adapters quickly to the set target temperature.

9

EN

Centrifuge 5418 / 5418 R — Operating manual

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2 Product description

2.4 Rotor FA-45-18-11

Before use of sample tubes, please note the manufacturer's specifications with regard to
centrifugation resistance (max. rcf).

Operating manual

Rotor
FA-45-18-11

Max. capacity Max. g-force (rcf)

or speed (rpm)
without adapter

Acceleration / deceleration

(2)

time

18 micro test tubes of 1.5/2.0 mL
each or spin columns.

With adapters:

• 0.2 mL PCR tubes

• 0.4 mL/0.5 mL micro test tubes

16,873 x g /
14,000 rpm

5418: 16 s / 18 s
5418 R: 13 s / 13 s

• 0.6 mL Microtainers

(1) Maximum load per rotor bore for adapter + tube + content.
(2) According to DIN 58 970 (device version: 230 V, 50 to 60 Hz).
(3) Aerosol tightness tested and certified by the Centre for Emergency Preparedness and
Response, Health Protection Agency, Porton Down (UK) (see certificate at the end of this
operating manual).

Max. load
per rotor

(1)

bore

3.75 g

Notes

• Aerosol-tight

(aluminum).

(3)

rotor lid

2.4.1 Rcf display and calculation

Use the rpm/rcf key to switch the display of centrifugation speed between rcf and rpm. Only
5418 R: For speeds  800 rpm only the lowest adjustable g-force (100 x g) is displayed upon

switching. The exact g-force (rcf) can be determined using the formula given below.
Ensure that the g-force (rcf) displayed upon switching is standardized to suit the rotor without an

adapter. When adapters are used, you can achieve the following maximum g-forces (rcf) at
maximum speed (rpm):

Adapters Max. centrifugation

without adapter 7.7 16,873
for 0.2 mL PCR tubes 5.6 12,271
for 0.4 mL micro test tubes 7.7 16,873
for 0.5 mL micro test tubes 6.6 14,462
for 0.6 mL Microtainers 7.7 16,873

To determine the g-force (rcf) for a specific adapter, you can calculate per DIN 58 970 using the
following formula:

rcf = 1.118 · 10
n: revolutions per minute (rpm)
r

: max. centrifuging radius in cm

max

Example

The 0.2 mL adapter has a maximum radius of 5.6 cm. At 5,000 rpm a maximum g-force of 1,565
x g is reached.

-5

· n2 · r

max

radius r

max

Max. g-force (rcf)

[cm]

10

3 Safety

3Safety

3.1 Intended use

CAUTION!

3.2 User profile

Centrifuge 5418 / 5418 R — Operating manual

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Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

The Centrifuge 5418 / 5418 R is intended exclusively for indoor use and for separating aqueous
solutions and suspensions of various densities in approved test tubes.

Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, function and precision of the device. Eppendorf cannot be held liable or accept
any liability for damage resulting from the use of incorrect or non-recommended accessories and
spare parts or from the improper use of such equipment.

 Only use accessories and original spare parts recommended by Eppendorf.

This device may only be operated by correspondingly trained specialist staff. This staff must have
carefully read the operating manual and be familiar with the function of the device.

EN

Operating manual

3.3 Application limits

3.3.1 Declaration concerning the ATEX directive (94/9/EC)

Danger of explosion!

 Do not operate the device in areas where work is completed with explosive substances.
 Do not use this device to process any explosive, radioactive or highly reactive substances.

DANGER!

3.3.2 Maximum service life for accessories

 Do not use this device to process any substances which could create an explosive

atmosphere.

Due to its design and the environmental conditions on the inside of the device, the Centrifuge
5418 / 5418 R is not suitable for use in a potentially explosive atmosphere.

The device must therefore only be used in a safe environment, such as in the open environment
of a ventilated laboratory or an extractor hood. The use of substances which may contribute to a
potentially explosive atmosphere is not permitted. The final decision with regard to the risks
connected with the use of such substances is the responsibility of the user.

Risk of injury from chemically or mechanically damaged accessories.

Even minor scratches and cracks can result in serious internal material damage.

 Protect all parts from mechanical damage.

CAUTION!

 Check accessories regularly.
 Do not use rotors or buckets with signs of corrosion or mechanical damage (e.g.

deformations).

 Do not use accessories whose maximum service life has been exceeded.

Accessory Maximum service life from first commissioning

Rotor lid 3 years

Requirement: The "click" when turning the rotor lid screw is still audible.
Plastic adapters 1 year
Outer sealing ring of the aerosol-tight rotor 50 autoclave cycles

For the rotor described here there is no limit for its service life, as long as the following conditions
are met: proper use, recommended maintenance and undamaged condition.

The date of manufacture is stamped on the rotors in the format 03/07 (= March 2007). This is for
information only and does not have any reference to the service life.

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Centrifuge 5418 / 5418 R — Operating manual

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3 Safety

3.4 Information on product liability

In the following cases, the protection provided by the device may be impaired. The liability for the
function of the device passes to the operator if:

• The device is not used in accordance with the operating manual.

• The device is used outside of the range of application described in the preceding chapters.

• The device is used with accessories or consumables (e.g., tubes) which are not

recommended by Eppendorf.

• The device is maintained or repaired by persons not authorized by Eppendorf.

• The owner has made unauthorized modifications to the device.

Operating manual

3.5 Warnings for intended use

Read the operating manual first and observe the following general safety instructions before
using the Centrifuge 5418 / 5418 R.

3.5.1 Personal injury or damage to the equipment

WARNING!

WARNING!

WARNING!

WARNING!

Electric shock from damage to device or mains cable.

 Only switch on the device if the device and the mains cable are undamaged.
 Only use devices that have been properly installed or repaired.

Lethal voltages inside the device.

 Ensure that the housing is always closed and undamaged so that no parts inside the device

can be contacted by accident.

 Do not remove the housing of the device.
 Do not allow any liquids to enter the inside of the housing.
 Do not allow the device to be opened by anyone except service personnel who have been

specifically authorized by Eppendorf.

Risk from incorrect supply voltage

 Only connect the device to voltage supplies which correspond with the electrical requirements

on the nameplate.

Damage to health due to handling infectious liquids and pathogenic germs.

 Observe the national regulations for handling these substances, the biological security level

of your laboratory, the material safety data sheets and the manufacturer's application notes.

 For the centrifugation of these substances, use suitable aerosol-tight closure systems.
 When working with pathogenic germs belonging to a higher risk group, more than one

aerosol-tight bioseal must be used.

 Wear your personal protective equipment (PPE).
 Follow the instructions regarding hygiene, cleaning and decontamination.
 For complete instructions regarding the handling of germs or biological material of risk group

II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health
Organization, current edition of the Laboratory Biosafety Manual).

12

WARNING!

Centrifuge lid can crush. Keep hands clear.

 When opening or closing the device lid, do not reach between the lid and device or into the

latching mechanism of the lid.

 Always open the centrifuge lid completely to prevent it from falling.

3 Safety

NOTICE!

NOTICE!

Centrifuge 5418 / 5418 R — Operating manual

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Damage to device by spilling liquids in the rotor or rotor chamber

1. Switch the device off.

2. Disconnect the device from the power supply.

3. Clean the device and the accessories carefully in accordance with the cleaning and
disinfection instructions in the operating manual.

4. If a different cleaning and disinfecting method is to be used, contact Eppendorf to ensure that
the intended method will not damage the device.

Damage to electronic components through formation of condensation.

After the device has been moved from a cool to a warmer environment, condensation can form
inside the device.

 Wait at least three hours before connecting it to the power supply.
 Only 5418: Alternative: let the device run for half an hour just before a short transport.

EN

Operating manual

3.5.2 Incorrect handling of the centrifuge

Damage from knocking against or moving the device during operation.

A rotor banging against the rotor chamber wall can cause considerable damage to the device and
rotor.

NOTICE!

3.5.3 Incorrect handling of the rotors

 Do not move or knock against the device during operation.

Risk of injury from improperly attached rotors and rotor lids.

 Centrifuge only with the rotor and rotor lid firmly tightened.
 If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be

CAUTION!

properly secured. Stop centrifugation immediately by pressing the start/stop key.

Risk of injury from asymmetric loading of rotors.

 Load rotors symmetrically with identical tubes.
 Only load adapters with suitable tubes.

CAUTION!

 Always use tubes of the same type (weight, material/density and volume).
 Check symmetric loading by balancing the adapters and tubes used with scales.

Risk of injury from overloaded rotor.

The Centrifuge 5418 / 5418 R is designed for the centrifugation of material with a max. density of

1.2 g/mL at maximum speed and volume and/or load.

CAUTION!

 Observe the information on each rotor relating to maximum load (adapter, tube and contents)

per rotor bore and make sure it is not exceeded.

NOTICE!

Damage to rotors from aggressive chemicals.

Rotors are high-quality components which withstand extreme stresses. This stability can be
impaired by aggressive chemicals.

 Avoid the use of aggressive chemicals, including strong and weak alkali, strong acids,

solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons,
concentrated saline solutions and phenol.

 If the rotor is contaminated by aggressive chemicals, clean it immediately using a neutral

cleaning agent. This applies to the rotor bores in particular.

13

EN

Operating manual

Centrifuge 5418 / 5418 R — Operating manual

3 Safety

3.5.4 Extreme strain on the centrifuging tubes

Risk of injury from overloaded tubes.

 Note the loading limits specified by the tube manufacturer.

CAUTION!

NOTICE!

NOTICE!

 Only use tubes which are approved by the manufacturer for the required rcf.

Risk from damaged tubes.

Damaged tubes must not be used, as this could cause further damage to the device and the
accessories and loss of the samples.

 Before use, carry out a visual check of all tubes for any damage.

Risk from open tube lids.

Open tube lids can break off during centrifugation and cause damage to the rotor as well as to
the centrifuge.

 Carefully close all tube lids before centrifuging.

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Hazard to plastic tubes from organic solvents.

When using organic solvents (e.g. phenol, chloroform) the density of plastic tubes is reduced,
i.e., the tubes could get damaged.

NOTICE!

NOTICE!

 Follow the manufacturer's information about the chemical resistance of tubes.

Sample tubes heat up.

In uncooled centrifuges the temperature in the rotor chamber, rotor and sample can rise to above
40°C dependent on the run time, g-force (rcf) or speed (rpm) and ambient temperature.

 Note that this can reduce the centrifugation resistance of the sample tubes.
 Please note the temperature resistance of the samples.

3.5.5 Aerosol-tight centrifugation

Risk to health due to limited aerosol tightness with incorrect rotor/rotor lid combination.

Aerosol-tight centrifugation is guaranteed only if the rotors and rotor lids intended for this purpose
are used. These are always indicated by the prefix FA.

WARNING!

 Always use rotors and rotor lids marked aerosol-tight together for aerosol-tight centrifugation.
 Only use aerosol-tight rotor lids in combination with rotors which are marked on the rotor lid.

Risk to health due to limited aerosol tightness in the event of incorrect use.

Autoclaving, mechanical stresses and contamination by chemicals or other aggressive solvents
can impair the aerosol-tightness of the rotors and rotor lid.

WARNING!

 Regularly check that the seals of aerosol-tight rotor lids are undamaged.
 Only use aerosol-tight rotor lids with undamaged and clean gaskets.
 Thinly brush the threads of the rotor lid screw with pivot grease (order no. Int.: 5810 350.050 /

North America: 022634330) after every proper autoclaving (121°C, 20 min.). Do not apply
the pivot grease to the gaskets.

 Replace the outer sealing ring of the rotor after 50 autoclave cycles.
 Aerosol-tight rotors should never be stored with rotor lids screwed on.

14

Centrifuge 5418 / 5418 R — Operating manual

3 Safety

3.6 Safety instructions on the device

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EN

Depiction Meaning Location

WARNING

General hazard point. Follow the operating
manual.

CAUTION

Always tighten up the rotor using the rotor key
supplied.

CAUTION

Close all tubes and use a rotor lid.

5418: Rear of the device
5418 R: Right side of the device

Top of device, below the centrifuge lid.

Top of device, below the centrifuge lid.

Operating manual

15

EN

Operating manual

4 Installation

4Installation

4.1 Selecting the location

NOTICE!

NOTICE!

Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

If a fault occurs, objects in the immediate vicinity of the devices could get damaged.

 In accordance with the recommendations of EN 61010-2-020, leave a safety distance of 

30 cm clear around the device during operation.

Damage from overheating.

 Do not place the device close to sources of heat (e.g., radiator, drying cabinet).
 Do not expose the device to direct sunlight.
 Ensure free circulation of air by maintaining a distance of at least 30 cm on all sides of the

device from adjacent devices or the wall and keep the underside of the device clear.

 Make sure that the vents in the device are always free of obstruction.

Select the location for the device according to the following criteria:

• Suitable power connection as per the name plate (230 V/120 V/100 V).

• Stable, horizontal and resonance-free lab bench. Weight of the device: 7.7 kg (5418) or 22 kg

(5418 R).

• A well ventilated environment which is protected from direct sunlight to prevent the device

from heating up more.

Centrifuge 5418 / 5418 R — Operating manual

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4.2 Installing the instrument

Centrifuge 5418 R: Compressor damage after improper transport.

 Only switch on the centrifuge 4 hours after installation.

NOTICE!

Perform the following steps in the sequence described.

1. Place the device on a suitable lab bench.

2. Allow the device to warm up for at least 3 hours (5418) or 4 hours (5418 R) to the ambient
temperature to prevent damage to the electronic components from condensation and damage
to the compressor (only 5418 R).

3. Check that the mains voltage and frequency match the requirements on the device type plate.

4. Connect the centrifuge to the mains and switch it on using the mains power switch on the
right side of the device.

• The display is active.

• Lid opens automatically

5. Use the details included in the scope of delivery to check that the delivery is complete.

6. Check all parts for any transport damage. Contact your dealer if any damage is found.

7. Only 5418 R: Insert the condensation water tray at the side of the device into the holder
provided (see Fig. 2 on p. 8).

Retain the packaging material and the transport protection device for subsequent transport or

Hint!

storage. See also the instructions relating to transport (see p. 30).

16

Centrifuge 5418 / 5418 R — Operating manual

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5 Operation

5Operation

5.1 Overview of operating controls

Abb. 3: Control panel of the Centr ifuge 5418 and Ce ntrifuge 5418 R.

Fig. 3: Control panel of the Centrifuge 5418 and Centrifuge 5418 R.

Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

Before using the Centrifuge 5418 / 5418 R for the first time, familiarize yourself with the operating
controls and the display.

EN

Operating manual

1 Switch the displayed centrifuging speed (rpm/rcf)

2Display

(see Rcf display and calculation on p. 10)

3 Release lid 4 Adjust the centrifuging duration

Only 5418 R: Press the time dial to start and stop

centrifugation.

5 Set the speed of centrifugation

6 Adjust the temperature (only 5418 R)

Only 5418 R: Press the speed dial to switch the

displayed centrifugation speed (rpm/rcf).

7 Start the temperature control run fast temp 

(only 5418 R)

8 Short spin centrifugation

(see Short spin centrifugation on p. 23)

9 Start and stop centrifugation

Abb. 4: Display of the Centrifug e 5418 and Centrifug e 5418 R.

Fig. 4: Display of the Centrifuge 5418 and Centrifuge 5418 R.

1 Centrifuging duration 2 g-force (rcf) or speed (rpm)

5418 R: Set value x 1000

3 Status of the centrifuge

: Centrifuge lid unlocked.
: Centrifuge lid locked.
(Flashing): centrifuging in progress.

4 At set rpm (only 5418 R)

: Start of run time when reaching 95% of the preset

g-force (rcf) or speed (rpm).

: Start of run time immediately.

5 Temperature (only 5418 R) 6 Status of the key lock (only 5418 R)

: Centrifuging parameters cannot be modified

unintentionally.

7 Status of the centrifugation speed display

rcf: g-force (relative centrifugal force)
rpm: revolutions per minute

: No key lock.

8 Status of the alarm (only 5418 R)

: Switched on.

No symbol: switched off.

17

EN

Operating manual

Centrifuge 5418 / 5418 R — Operating manual

5 Operation

5.2 Preparing for centrifugation

5.2.1 Switching on the centrifuge

1. Switch the centrifuge on, using the mains switch.
After switching on at the mains power switch, the centrifuge lid opens automatically.

2. Open the closed centrifuge lid by pressing the open key.
The parameter settings of the last run are displayed.

5.2.2 Inserting the rotor

If handled incorrectly, the rotor can fall over.

The rotor lid screw may become loose if it is used to retain the rotor.

NOTICE!

 Always grasp the rotor with both hands for holding or transport.

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1. Place rotor vertically onto the motor shaft.

2. Insert the rotor key supplied into the rotor nut.

3. Turn rotor key clockwise until the rotor nut is firmly tightened.

5.2.3 Loading the rotor

Risk of injury from asymmetric loading of rotors.

 Load rotors symmetrically with identical tubes.

CAUTION!

 Only load adapters with suitable tubes.
 Always use tubes of the same type (weight, material/density and volume).
 Check symmetric loading by balancing the adapters and tubes used with scales.

Risk from damaged or overloaded tubes!

 When loading the rotor, note the safety instructions with regard to hazards from overloaded or

CAUTION!

To load the rotor, proceed as follows:

1. Check the maximum load (adapter, tube and contents) per rotor bore.

2. Load rotor and adapters only with the tubes intended for them.

3. Insert tubes opposite each other in pairs into the rotor bores. For symmetrical loading, tubes

damaged tubes (see Warnings for intended use on p. 12)

The maximum load is 3.75 g per rotor bore. This information can also be found on the rotor.

that are opposite each other must be of the same type and contain the same filling quantity.

18

In order to minimize weight differences between filled sample tubes, we recommend taring
with a scale. This will reduce wear on the drive and cut running noise.

5 Operation

5.2.4 Closing the rotor lid

Centrifuge 5418 / 5418 R — Operating manual

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EN

With the rotor FA-45-18-11 centrifugation is also possible without a rotor lid.

Hint!

Please also note:

Operating manual

• The tube lids must be closed securely.

• Without the rotor lid, the rotor is not aerosol-tight.

• The centrifugation is slightly louder.

• Spin columns must always be centrifuged with a rotor lid.

1. Check that the outer sealing ring is fitted properly in the
groove.

2. Fit the rotor lid vertically on the rotor.

3. Lock the rotor by turning the red rotor lid screw clockwise
beyond an audible click until it can be turned no further.

The rotor is not properly closed until an audible click
is heard!

5.2.5 Closing the centrifuge lid

Centrifuge lid can crush. Keep hands clear.

 When opening or closing the device lid, do not reach between the lid and device or into the

latching mechanism of the lid.

WARNING!

 Always open the centrifuge lid completely to prevent it from falling.

1. Check correct attachment of rotor and rotor lid.

2. Push down the centrifuge lid until the lid latch engages and the lid is automatically closed.

The centrifuge will close automatically.
The display shows the symbol .
Only 5418: The open key lights blue.

19

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Centrifuge 5418 / 5418 R — Operating manual

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5 Operation

5.3 Cooling (only 5418 R)

5.3.1 Temperature adjustment

 Set the temperature using the temp arrow keys from 0°C to +40°C.

5.3.2 Temperature display

If the rotor is stopped: Set temperature
During centrifugation: Actual temperature

Operating manual

5.3.3 Temperature monitoring

After the set temperature has been reached the centrifuge responds as follows to temperature
fluctuation during centrifugation:

Deviation from the target value Action

ΔT > 3°C Temperature display flashes.
ΔT > 5°C Periodic warning sound and visual indication

Error 18. Centrifugation is stopped
automatically.

5.3.4 Fast Temp

This function can be used to start a temperature control run directly without samples with a rotor
and temperature-specific speed in order to quickly adjust the rotor chamber, incl. rotor and
adapters, up to the set target temperature.

Requirement

• The centrifuge is switched on.

• The rotor and rotor lid are properly attached.

• The centrifuge lid is closed.

• The temperature and g-force (rcf) or speed (rpm) are set for the subsequent centrifugation

(see Centrifugation on p. 21).

1. Press the fast temp key.
The display shows FT as well as the current temperature and g-force (rcf) or speed (rpm).

The temperature control run ends automatically when the target temperature has been
reached. A periodic signal tone sounds.

The cooling time from room temperature (~ 23 °C) to 4°C takes approx. 16 min.

2. Press the start/stop key to terminate the temperature control run early.

After the set temperature has been reached and the temperature control run is complete, the
centrifuge keeps the rotor chamber, with the centrifuge lid closed, at the set temperature, if the
temperature is below the ambient temperature. Irrespective of the target temperature, however,
this continuous cooling does not go below 4°C to prevent the rotor chamber from freezing.

20

The centrifuge stops the run automatically when the rotor has reached the set temperature.

Hint!

Therefore, there may be a delay between the display of the achieved set temperature and the
automatic end of the temperature control run.

5 Operation

5.3.5 Continuous cooling

When the rotor is stopped the rotor chamber is kept at the target temperature when the following
prerequisites are met:

• The centrifuge is switched on.

• The centrifuge lid is closed.

• The target temperature is below the ambient temperature.

During continuous cooling the following applies:

• The target temperature is displayed.

• Irrespective of the target temperature, continuous cooling does not go below 4°C to prevent

• Because the rotor does not rotate during this process the temperature adjustment is slower.

To end continuous cooling, open the centrifuge lid.
If the centrifuge is not used for more than 8 hours, the continuous cooling is switched off

automatically. This protects against ice formation in the rotor chamber and in the tubes as well as
increased condensation in the device.

The display shows the set temperature of the rotor chamber.
With fast temp you can quickly reach the desired temperature again (see p. 20).

Centrifuge 5418 / 5418 R — Operating manual

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EN

Operating manual

the rotor chamber and the samples from freezing and increased condensation in the device.

5.4 Centrifugation

Risk from incorrectly-loaded rotors and damaged/overloaded tubes!

 Before commencing centrifugation, follow the safety instructions relating to hazards from

asymmetrically loaded and/or overloaded rotors and from overloaded, damaged and/or open

CAUTION!

tubes (see Warnings for intended use on p. 12).

Risk of injury from improperly attached rotors and rotor lids.

 Centrifuge only with the rotor and rotor lid firmly tightened.

CAUTION!

5.4.1 Centrifuging with time preset

 If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be

properly secured. Stop centrifugation immediately by pressing the start/stop key.

Before using the Centrifuge 5418 / 5418 R for the first time, familiarize yourself with the operating
controls and the display (see Overview of operating controls on p. 17).

Each of the centrifuging variants described here must be preceded by the preparation described
above (see Preparing for centrifugation on p. 18).

Only 5418 R: Please also note the instructions on cooling (see p. 20).

Perform the following steps in the sequence described.

1. Use time to set the run time.

2. Only 5418 R: Use temp to set the temperature.

3. Use speed to set the g-force (rcf) or speed (rpm).

4. Press start/stop to start centrifuging.

21

EN

Operating manual

Centrifuge 5418 / 5418 R — Operating manual

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5 Operation

During centrifugation

• In the display flashes while the rotor is running.

• The remaining run time is displayed in minutes, in 30s increments below ten minutes. The

last minute is counted down in seconds.

• Only 5418 R: The actual temperature is displayed.

• The current g-force (rcf) or rotor speed (rpm) is displayed.

• The fast temp (only 5418 R), open and short keys are blocked during centrifugation.

• During the run you can modify the total run time, the temperature (only 5418 R), the speed

and the rpm/rcf display. Only 5418 R: To change the centrifugation parameters, press the
short key first.

The values flash in the display during the change. The new parameters are adopted
immediately. When the time is changed during a run, the time which has already elapsed
is taken into account. Note that the shortest new total run time which can be set is the time
which has already elapsed plus 2 minutes.

• You can also terminate the centrifugation before the set run time has elapsed by pressing

the start/stop key.

End of centrifugation

• After completion of the set time, the centrifuge stops automatically. During braking the

elapsed centrifugation time is displayed flashing. When the rotor stops a signal tone is
sounded.

• Only 5418: The centrifuge lid opens automatically. The display shows the symbol .

• Only 5418 R: The centrifuge lid remains closed to maintain the sample temperature. You

can open it by pressing the open key.

5. Remove centrifuge contents.

5.4.2 Centrifuging in continuous operation

Perform the following steps in the sequence described.

1. Use time to set the continuous run.
The continuous run function can be set above 9:59 h or under 30 s (5418) or above 99 min or

under 0.5 min (5418 R). The timer shows oo to indicate continuous operation.

2. Only 5418 R: Use the temp arrow keys to adjust the temperature.

3. Use speed to set the g-force (rcf) or speed (rpm).

4. Press start/stop to start centrifuging.
In the display flashes while the rotor is running.

Time is counted upwards, first in 30-second increments and then in minute increments from
ten minutes.

5. Press start/stop to end centrifuging after the desired time period.

• During the braking process, centrifuging duration flashes in the display.

• When the rotor stops a signal tone is sounded.

• Only 5418: The centrifuge lid opens automatically. The display shows the symbol .

• Only 5418 R: The centrifuge lid remains closed to maintain the sample temperature. You

can open it by pressing the open key.

6. Remove centrifuge contents.

22

5 Operation

5.4.3 Short spin centrifugation

5418: You can carry out a short spin run with the maximum g-force (rcf) or speed (rpm).
5418 R: You can carry out a short spin run with the currently set, or maximum, g-force (rcf) or

speed (rpm). Set this short spin mode as described in the following section.

Selecting short spin mode (only 5418 R)

1. Press the short key while the centrifuge lid is open.
The current mode is displayed:

• Display 1 – 14 t (run at preselected speed)

• Display 14 t (run at maximum speed of 14,000 rpm)

2. When the centrifuge lid is open, press the short key for longer than 2 s to switch between
these modes.

Performing short spin centrifugation

1. Only 5418 R: A short run at preselected g-force (rcf) or speed (rpm) can be set directly using
the speed dial.

2. Only 5418 R: Use the temp arrow keys to adjust the temperature.

3. Start short spin run: Hold down the short key.

• In the display flashes while the rotor is running.

• The time is counted upwards in seconds.

• During short run centrifuging all other keys are blocked.

4. End short spin run: Release the short key.

• During the braking process, centrifuging duration flashes in the display.

• Only 5418: The centrifuge lid opens automatically. The display shows the symbol .

• Only 5418 R: The centrifuge lid remains closed to maintain the sample temperature. You

can open it by pressing the open key.

5. Remove centrifuge contents.

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

EN

Operating manual

During the braking process, centrifuging can be restarted up to two more times by pressing the

Hint!

5.4.4 Removing the rotor

short key again.

1. Turn the rotor nut counterclockwise using the rotor key supplied.

2. Remove rotor by lifting vertically.

3. Only 5418 R: Switch off the centrifuge after use and empty the condensation water tray (pull
out from the left or right side of the device). Leave centrifuge lid fully opened and protect it
against closing.

5.5 Standby mode (only 5418)

If the centrifuge has not been used for 15 min, it switches to standby mode. The EP logo then
appears in the display. When a button or knob is used or the centrifuge lid is closed, the
centrifuge is reactivated and ready for operation.

5.6 Special functions (only 5418 R)

Function Status of centrifuge lid Press > 2 s key Display

Modify parameter during the cycle. closed short Flashes 5 s
Enable/disable signal tone.

Enable/disable key lock.
At set rpm (Fig. 4 on p. 17)

open

open
open

open

short + open
time

/

/

23

EN

Centrifuge 5418 / 5418 R — Operating manual

6 Maintenance

6 Maintenance

6.1 Prepare cleaning / disinfection

Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

Clean at least once a week and clean if the accessible surfaces of the device and its accessories
are acutely contaminated.

Clean the rotor regularly. This way the rotor is protected and the durability is prolonged.
Furthermore, observe the notes on decontamination (see Decontamination before shipping on

p. 26) when the device is sent to the authorized Technical Service for repairs.
The procedure described in the following chapter applies for the cleaning as well as for the

disinfection or decontamination. The additional required steps are described in the following
table:

finale Korrektur

Operating manual

Cleaning Disinfecting / decontamination

1. For cleaning the accessible surfaces of the
device and the accessories use a mild
cleaning fluid.

2. Carry out the cleaning as described in the
following chapter.

If you have any further questions regarding the cleaning and disinfection or decontamination or

Hint!

regarding the cleaning fluid to be used, contact the Eppendorf AG Application Support. The
contact details are provided on the back of this manual.

6.2 Perform cleaning / disinfection

Electric shock as a result of penetration of liquid.

 Switch off the device and disconnect it from the power supply before starting cleaning or

disinfecting.

DANGER!

 Do not allow any liquids to enter the inside of the housing.
 Do not complete a spray disinfection on the housing.
 Only reconnect the device to the power supply once it is completely dry.

1. Choose the disinfection method which
corresponds to the legal regulations and
guidelines in place for your range of
application. For example, use alcohol
(ethanol, isopropanol) or alcohol-based
disinfectants.

2. Carry out the disinfection or
decontamination as described in the
following chapter.

3. Then clean the device and the accessories.

24

NOTICE!

NOTICE!

NOTICE!

Damage from the use of aggressive chemicals.

 Do not use any aggressive chemicals on the device or its accessories, such as strong and

weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.

 If the device becomes contaminated with aggressive chemicals, clean it immediately with a

mild cleaning agent.

Corrosion from aggressive cleaning agents and disinfectants.

 Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes.
 Do not incubate the accessories in aggressive cleaning agents or disinfectants for prolonged

periods.

Damage from UV and other high-energy radiation.

 Do not use UV, beta, or gamma radiation or any other high-energy radiation source for

disinfecting.

6 Maintenance

Autoclave

Hint!

Hint!

Rotor, rotor lid and adapter can be autoclaved (121°C, 20 min).
After a maximum of 50 autoclave cycles, replace the sealing ring in the lid groove of the

aerosol-tight rotor lid.

Aerosol-tightness

Check that the seals are intact before use.
Replace the sealing ring in the lid groove of the aerosol-tight rotor lid when worn. Replace the

rotor lids of aerosol-tight rotors when the sealing ring on the lid screw is worn. The sealing rings
require regular care to protect the rotors.

Aerosol-tight rotors should never be stored with lids screwed on!
In order to prevent damage, lightly lubricate the lid thread of the aerosol-tight rotors with pivot

grease (order no. Int.: 5810 350.050 / North America: 022634330) on a regular basis.

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

EN

Operating manual

6.2.1 Clean/disinfect device

1. Switch off the device from the mains power switch while the lid is opened and remove the
mains power switch from the power supply.

2. Unscrew the rotor nut by turning it counterclockwise with the rotor key.

3. Remove rotor.

4. Use the agents specified above (see p. 24) to clean and disinfect the device and the rotor
chamber.

5. Wipe all accessible surfaces of the device and the accessories, including the power cable,
with a moist cloth.

6. Thoroughly clean the rubber seals of the rotor chamber with water.

7. Rub the dry rubber seals with glycerine or talcum powder to prevent them from becoming
brittle. Other components of the device, such as the lid latch, must not be lubricated.

8. Clean the motor shaft with a soft, dry and lint-free cloth.

9. Check device and accessories for corrosion and damage.

6.2.2 Clean/disinfect rotor

1. Clean rotor, rotor lid and adapters with the aforementioned agents and disinfect them
(see p. 24).

2. Use a bottle brush to clean and disinfect the rotor bores.

3. In order to clean and disinfect the rotor lid, remove the sealing ring. Clean the groove and the
sealing ring below it as well.

4. Rinse rotor, rotor lid and adapters thoroughly with water. Pay particular attention to the rotor
bores of the fixed-angle rotors.

5. Place rotor and accessories onto a cloth to dry. Place fixed angle rotors with the rotor bores
facing downwards to allow the bores to dry as well.

6. Correctly reinsert the sealing ring of the rotor lid in the clean and dry groove.

7. Clean the rotor cone with a soft, dry and lint-free cloth and inspect for damage.

8. Place the dry rotor onto the motor shaft.

9. Tighten the rotor nut firmly by turning it clockwise with the rotor key.

25

EN

Centrifuge 5418 / 5418 R — Operating manual

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6 Maintenance

6.3 Additional service instructions for Centrifuge 5418 R

Empty and clean the condensation water tray regularly and especially after liquid spillage in



the rotor chamber. Pull out the condensation water tray from the left or right side of the
centrifuge.

 Clear the rotor chamber regularly of ice formations by thawing, either by leaving the centrifuge

lid open or carrying out a brief temperature control run at approx. 30 °C.

 Wipe up condensate in the rotor chamber. To do so, use a soft absorbent cloth.
 Remove dust deposits from the ventilation slits of the centrifuge using a brush or swab at the

latest every six months. First switch off the device and remove the power plug.

Operating manual

6.4 Glass breakage

Note when using glass tubes that the danger of breakage of glass increases with an increasing

Hint!

g-force (rcf) or speed (rpm). Please note the manufacturer's information on the recommended
centrifugation parameters (loading and speed).

Broken glass scratches the surfaces of the rotor chamber and the accessories (rotor, rotor lid and
adapter) so that their chemical resistance is reduced. Therefore, a fine, black metal abrasion
develops in the rotor chamber due to the air turbulence which, in addition to causing damage to
the rotor chamber and the accessories, can also contaminate the samples.

 Carefully remove all splinters and glass powder from the rotor chamber and accessories

 If required, replace the adapters to prevent any further damage.
 Check rotor bores regularly for residues or damage.

6.5 Fuses

The fuse holder is located under the mains power socket (5418) or on the left next to the mains
switch (5418 R).

1. Disconnect the mains plug.

2. Remove the fuse holder.

(rotor, rotor lid and adapter) when breakage of glass occurs.

Both fuses are now accessible and can be replaced.

6.6 Decontamination before shipping

If you are shipping the device to the authorized Technical Service for repairs or to your authorized
dealer for disposal please note the following:

Risk to health from contaminated device

1. Follow the instructions in the decontamination certificate. It is available in PDF format on our

WARNING!

26

homepage (www.eppendorf.com/decontamination)

2. Decontaminate all the parts you want to dispatch.

3. Enclose the fully-completed decontamination certificate for returned goods (including the
serial number of the device) with the dispatch.

.

Centrifuge 5418 / 5418 R — Operating manual

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7 Troubleshooting

7 Troubleshooting

7.1 General errors

Symptom / message Cause Remedy

No display. No mains connection.  Check the mains power connection.
No display. Power failure.

Lid of the device cannot
be opened.

Lid of the device cannot
be opened.

Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

If the suggested measures fail repeatedly, please contact Technical Service. You can find the
contact addresses at the end of this operating manual or on the Internet under
www.eppendorf.com

.

 Check the mains fuse for the device

(see Fuses on p. 26).

 Check the mains fuse for the laboratory.

Rotor is still running.

Power failure. 1. Check the mains fuse for the device

 Wait for the rotor to stop.

(see Fuses on p. 26).

2. Check the mains fuse for the laboratory.

3. Activate the emergency lid release
(see p. 29).

EN

Operating manual

Device cannot be started. Lid of the device is not closed.
Device shakes when it

starts up.

Centrifuge brakes during
a short run centrifugation,
although the short key is
pressed.

Temperature display
flashes.

(only 5418 R)

Rotor is unsymmetrically loaded. 1. Stop the device and load symmetrically.

The short key was released briefly more than
twice (protective function for the drive).

Temperature variation from nominal value:
ΔT > 3°C.

 Close the lid of the device.

2. Restart the device.

 Press the short key continuously during a

short run centrifugation.

 Check the settings.
 Check unhindered air circulation through

the vents.

 Thaw ice or switch off device and allow it

to cool down.

7.2 Error messages

If one of the following error messages appears, proceed as follows:

1. Remove fault (see Remedies).

2. If necessary, repeat centrifugation.

Symptom / message Cause Remedy
LID ERROR (5418) /

LID (5418 R)
LID ERROR (5418) /

LID (5418 R)

Centrifuge lid cannot be locked.

Centrifuge lid cannot be released. 1. Switch the device off and back on.

 Try to close the centrifuge lid again.

2. Press the open key.

If the error occurs again:

LID ERROR (5418) /
LID (5418 R)

Centrifuge lid must not be released during a
run.

1. Switch off the centrifuge.

2. Activate the emergency lid release
(see Emergency lid release on p. 29).

 Wait for the rotor to stop.

27

EN

Operating manual

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

7 Troubleshooting

Symptom / message Cause Remedy
INT Mains power failure during a run.  Check the mains connection.
NO RPM (5418) /

Error 3 (5418 R)
Error 5

(only 5418 R)

Err 6 (5418) /
Error 6 (5418 R)

Error in speed measuring system or drive
overheated.

Prohibited opening of lid or lid switch is
defective during a run.

Drive error.

 Leave the device switched on until the

error message disappears (10 s or 6 min).

1. Wait for the rotor to stop.

2. Open and close again the lid of the
device.

3. Repeat the run.

 Repeat the run.
 If this error message appears again,

switch centrifuge off and back on again
after > 20 s.

Error 6

(only 5418 R)

Err 7 (5418) /
Error 7 (5418 R)

Err 8 (5418) /
Error 8 (5418 R)

Err 9 to 17 (5418) /
Error 9 to 17 (5418 R)

Error 18

(only 5418 R)

Error 19

(only 5418 R)

Error 20

(only 5418 R)

Error 21

(only 5418 R)

Error 24

(only 5418 R)

Drive overheated.

Major deviation in the speed control. 1. Wait for the rotor to stop.

Drive error. 1. Wait for the rotor to stop.

Electronics error.

Too high temperature variation from nominal
value in the rotor chamber.

 Allow the drive to cool down for at least 15

min.

2. Tighten the rotor.

2. Repeat the run.

 Switch the centrifuge off and back on

again after > 20 s.

 Check the settings.
 Check unhindered air circulation through

the vents.

 Thaw ice or switch off device and allow it

to cool down.

Cooling circuit is overheated.

Temperature sensor in rotor chamber faulty.

Temperature sensor at capacitor faulty.

Cooling unit fault, e.g., overheating.

 Check unhindered air circulation through

the vents and allow device to cool down.

 Switch the centrifuge off and back on

again after > 20 s.

 Switch the centrifuge off and back on

again after > 20 s.

 Allow the centrifuge to cool down and

repeat the run.

28

7 Troubleshooting

7.3 Emergency lid release

If the centrifuge lid cannot be opened, you can activate the emergency lid release manually.

Risk of injury from rotating rotor.

 Wait for the rotor to stop before activating the emergency lid release.

WARNING!

7.3.1 Centrifuge 5418

 To check, look through the inspection glass in the centrifuge lid.

1. Disconnect the mains plug.

2. Loosen the plastic cover for the emergency lid release.
This is located behind the front left device foot in the base plate (Fig. 1 on p. 8).

3. Pull the cord vertically downwards.
The centrifuge lid is unlocked.

4. Before closing the centrifuge lid again: push the cord completely into the housing and insert
the plastic cover in the base plate.

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

EN

Operating manual

7.3.2 Centrifuge 5418 R

1. Disconnect the mains plug.

2. Rotate the plastic cover of the emergency lid release 45° counterclockwise with a suitable

3. Enter the centrifuge rotor key into the hexagonal opening at rear until some resistance can be

4. Slightly press and turn the rotor key clockwise.

5. Open the centrifuge lid.

6. Remove the rotor key and put the plastic cover back on rotating it 45° clockwise.

tool (e.g. screwdriver) and remove it.
The plastic cover is located on the right side of the device (Fig. 2 on p. 8).

felt.

This will release the centrifuge lid.

29

EN

Centrifuge 5418 / 5418 R — Operating manual

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8 Transport, storage and disposal

8 Transport, storage and disp osal

8.1 Transport

Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

Only transport the device in the original packaging.


 Use a transport aid for transporting over longer distances.

Air temperature Rel. humidity Air pressure

General transportation -25 to 60 °C 10 to 75% 30 to 106 kPa
Air freight -20 to 55 °C 10 to 75% 30 to 106 kPa

Operating manual

8.2 Storage

8.3 Disposal

Air temperature Rel. humidity Air pressure

In transport packaging -25 to 55 °C 10 to 75% 70 to 106 kPa
Without transport

packaging

In case the product is to be disposed of, the relevant legal regulations must be observed.

Information on the disposal of electrical and electronic devices in the European
Community:

The disposal of electrical devices is regulated within the European Community by national
regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic
equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005 on a
business-to-business basis, to which this product is assigned, may no longer be disposed of in
municipal or household waste. To document this they have been marked with the following
identification:

-5 to 45 °C 10 to 75% 70 to 106 kPa

30

Because disposal regulations may differ from one country to another within the EU please
contact your supplier if necessary.

9 Technical data

9 Technical data

9.1 Power supply

Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

Tab. 1: Centrifuge 5418

Mains power connection 230 V, 50 to 60 Hz

Current consumption: 1.4 A (230 V)

Power consumption: max. 170 W
EMC: Interference emission (radio

interference)
EMC: Noise immunity EN 61326 - performance characteristic B
Overvoltage category: II
Fuses: 2.5 AT (230 V)

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

EN

Operating manual

120 V, 50 to 60 Hz
100 V, 50 to 60 Hz

2.8 A (120 V)

3.4 A (100 V)

EN 61326 - category B

5.0 AT (120 V / 100 V)

Tab. 2: Centrifuge 5418 R

Mains power connection 230 V, 50 to 60 Hz

Current consumption: 1.4 A (230 V)

Power consumption: max. 320 W
EMC: Interference emission (radio

interference)
EMC: Noise immunity EN 61326 - performance characteristic B
Overvoltage category: II
Fuses: 2.5 AT (230 V)

9.2 Ambient conditions

Environment: For indoor use only.
Ambient temperature: Centrifuge 5418: 2 to 40°C

Max. relative humidity: 75 %, non-condensing humidity.

120 V, 50 to 60 Hz
100 V, 50 to 60 Hz

2.8 A (120 V)

3.0 A (100 V)

EN 61326 - category B

6.25 AT (120 V / 100 V)

Centrifuge 5418 R: 15 to 35°C

Atmospheric pressure: Use up to an altitude of 2000 m above MSL.
Degree of contamination: 2

31

EN

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

9 Technical data

9.3 Weight / dimensions

Tab. 3: Centrifuge 5418

Dimensions: Width: 208 mm (8.19 in.)

Depth: 300 mm (11.8 in.)

Height: 210 mm (8.27 in.)
Weight without rotor: 7.7 kg
Noise level: < 51 dB(A) *

Operating manual

Tab. 4: Centrifuge 5418 R

Dimensions: Width: 298 mm (11.73 in.)

Weight without rotor: 22 kg
Noise level: < 57 dB(A) *

*) The noise level was measured frontally in a sound measuring room with accuracy class 1 at a
distance of 1 m from the device and at lab bench height.

9.4 Application parameters

Rotor Acceleration and deceleration times

FA-45-18-11 5418:

These values were calculated at 23°C.

Run time:
(5418)

Depth: 463 mm (18.23 in.)

Height: 250 mm (9.84 in.)

according to DIN 58 970

5418 R:
230 V: 16 s / 18 s
120 V: 16 s / 18 s
100 V: 18 s / 18 s

30 s to 9:59 h, as well as infinity (oo),
adjustable up to 10 min in 30 s increments,

thereafter in 1 min increments.

230 V: 13 s / 13 s

120 V: 13 s / 13 s

100 V: 13 s / 13 s

32

Run time:
(5418 R)

Temperature:
(only 5418 R)

Relative centrifugal force (RZB or rcf): 1 to 16,873 x g,

Speed: 100 to 14,000 rpm,

Max. load: 18 micro test tubes of 2.0 mL each.
Max. kinetic energy: 2,600 Nm
Test log mandatory: No
Permitted density of the material for

centrifuging (at max. g-force (rcf) or
speed (rpm) and max. load):

0.5 min to 99 min, as well as infinity (oo),
adjustable up to 10 min. in 0.5 min.

increments, thereafter in 1 min. increments.
0°C to 40°C

adjustable in 100 x g increments.

adjustable in 100 rpm increments.

1.2 g/mL

Centrifuge 5418 / 5418 R — Operating manual

10 Ordering information

10 Ordering information

10.1 Centrifuge 5418

Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

finale Korrektur

EN

Order No.
(International)

5418 000.017

-

Order No.
(North America)

022620321
022620304

Description

Centrifuge 5418

with rotor FA-45-18-11 incl. rotor lid
230 V / 50 - 60 Hz
120 V / 50 - 60 Hz, with US-plug

10.2 Centrifuge 5418 R

Order No.
(International)

5401 000.013

-

Order No.
(North America)

5401000013
5401000137

Description

Centrifuge 5418 R

with rotor FA-45-18-11 incl. rotor lid
230 V / 50 Hz
120 V / 50 - 60 Hz, with US-plug

10.3 Accessories

Order No.
(International)

5418 707.005 022652061

5418 708.001 022652087

5418 709.008 022652109

5425 715.005
5425 717.008
5425 716.001

5416 301.001 022634305

5703 350.102 022639609
5401 850.076 5401850076 Tray for condensation water (only 5418 R)

*) Aerosol tightness tested and certified by the Centre of Emergency Preparedness and Response, Health Protection
Agency, Porton Down (UK).

Order No.
(North America)

022636260
022636243
022636227

Description

Rotor FA-45-18-11

aerosol-tight*, angle 45°, 18 places, max. tube diameter 11 mm, incl. rotor lid
(aluminum)

Rotor lid for FA-45-18-11

aerosol-tight*, aluminum

Seal for rotor lid FA-45-18-11

5 pieces

Adapter

used in FA-45-18-11
for 0.2 mL PCR tubes, set of 6
for 0.4 mL tubes, set of 6
for 0.5 mL tubes and 0.6 mL Microtainer, set of 6

Rotor key

Standard

Captain Eppi rotor key holder

1 piece

Operating manual

10.4 Fuses

10.4.1 Fuses for Centrifuge 5418 Order No.

(International)

5425 351.003
5425 353.006

10.4.2 Fuses for Centrifuge 5418 R Order No.

(International)

5425 351.003
5426 355.100

Order No.
(North America)

022668188
022668226

Order No.
(North America)

022668188
022668200

Description

Fuses

2.5 AT (230 V), 2 pieces
5 AT UL (100 V/120 V), 2 pieces

Description

Fuses

2.5 AT (230 V), 2 pieces

6.25 AT (100 V/120 V), 2 pieces

33

Centrifuge 5418 / 5418 R — Operating manual

EG-Konformitätserklärung

EC Conformity Declaration

Das bezeichnete Produkt entspricht den einschlägigen grundlegenden Anforderungen der

aufgeführten EG-Richtlinien und Normen. Bei einer nicht mit uns abgestimmten Änderung des

Produktes oder einer nicht bestimmungsgemäßen Anwendung verliert diese Erklärung ihre Gültigkeit.

The product named below fulfills the relevant fundamental requirements of

the EC directives and standards listed. In the case of unauthorized modifications to the product

or an unintended use this declaration becomes invalid.

Produktbezeichnung, Product name:

Produkttyp, Product type:

Einschlägige EG-Richtlinien/Normen, Relevant EC directives/standards:

Vorstand, Board of Management: Projektmanagement, Project Management:

Hamburg, Date:

Eppendorf AG · Barkhausenweg 1 · 22339 Hamburg · Germany

0015 033.509-02

Laborzentrifuge / Laboratory Centrifuge

Centrifuge 5418 / 5418 R

einschließlich Zubehör / including accessories

2006/95/EG, EN 61010-1, EN 61010-2-20

2004/108/EG, EN 55011/B, EN 61000-6-1, EN 61000-3-2, EN 61000-3-3, EN 61326-1

98/79/EG, EN 14971, EN 61010-2-101, EN 61326-2-6

08.10.2009

5418 900.301-03

finale Korrektur

11 Declarations and Certificates

8 Declarations and Certificates

Operating manual — Centrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

125

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

11 Declarations and Certificates

126

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

11 Declarations and Certificates

127

Centrifuge 5418 / 5418 R — Operating manual

finale Korrektur

11 Declarations and Certificates

&HUWLILFDWH1XPEHU
5HSRUW5HIHUHQFH
,VVXH'DWH

2009 October 23



This is to certify that

representative samples of



Standard(s) for Safety:

Additional Information:

20091023-E215059
E215059, 2001 April 5

Issued to:

EPPENDORF A G

BARKHAUSENWEG 1
22339 HAMBURG GERMANY

LABORATORY USE ELECTRICAL EQUIPMENT

5415R, 5418R

Have been investigated by Underwriters Laboratories Inc.® (UL) or any authorized
licensee of UL in accordance with the Standard(s) indicated on this Certificate.

UL 3101-1, First Edition
UL 3101-2-20, First Edition
CAN/CSA-C22.2 No. 1010.1-92
CAN/CSA-C22.2 No.1010.2.020-92, CAN/CSA-C22.2 No. 1010.2.020A-97

See UL On-line Certification Directory at WWW.UL.COM for additional
information

3DJHRI

128

Only those products bearing the UL Listing Mark for the US and Canada should be considered as being covered by
UL's Listing and Follow-Up Service meeting the appropriate requirements for US and Canada.

The UL Listing Mark for the US and Canada generally includes: the UL in a circle symbol with “C” and “US” identifiers:

the word “LISTED”; a control number (may be alphanumeric) assigned by UL; and the product category name (product

identifier) as indicated in the appropriate UL Directory.

Look for the UL Listing Mark on the product

Issued by: Reviewed by:

Thomas Huber, Engineer Walter Hofmair, Staff Engineer

Underwriters Laboratories Inc. Underwriters Laboratories Inc.

Centrifuge 5418 / 5418 R — Operating manual

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11 Declarations and Certificates

129

Eppendorf offices

AUSTRALIA & NEW ZEALAND

Eppendorf South Pacific Pty. Ltd.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.com.au

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Eppendorf offices

AUSTRIA

Eppendorf Austria GmbH
Phone: [phone] - 0
Fax: [phone] - 20
E-mail: [e-mail]
Internet: www.eppendorf.at

BRAZIL

Eppendorf do Brasil Ltda.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.com.br

CANADA

Eppendorf Canada Ltd.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorfna.com

FRANCE

Eppendorf France S.A.R.L.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.fr

ITALY

Eppendorf s.r.l.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.it

CHINA

Eppendorf China Ltd.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.cn

GERMANY

Eppendorf Vertrieb
Deutschland GmbH
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.de

JAPAN

Eppendorf Co. Ltd.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.jp

CZECH REP. & SLOVAKIA

Eppendorf Czech & Slovakia s.r.o.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.cz /
www.eppendorf.sk

INDIA

Eppendorf India Limited
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.co.in

NORDIC

Eppendorf Nordic Aps
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.dk

SOUTH & SOUTHEAST ASIA

Eppendorf Asia Pacific Sdn. Bhd.
Phone: [phone]
Fax: [phone]
E-mail:
[e-mail]
Internet: www.eppendorf.com.my

UNITED KINGDOM

Eppendorf UK Limited
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.co.uk

SPAIN

Eppendorf Ibérica S.L.U.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.es

USA

Eppendorf North America, Inc.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorfna.com

SWITZERLAND

Vaudaux-Eppendorf AG
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.eppendorf.ch

OTHER COUNTRIES

Internet: www.eppendorf.com/
worldwide

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Your local distributor: www.eppendorf.com/worldwide

Eppendorf AG · 22331 Hamburg · Germany · Tel: [phone] · Fax: [phone] · E-Mail: [e-mail]

Eppendorf North America, Inc. · 102 Motor Parkway, Suite 410 · Hauppauge, NY 11788-5178 · USA

Tel: [phone] · Toll free phone: [phone] · Fax: [phone] · E-Mail: [e-mail]

Application Support

Europe, International: Tel: [phone] · E-Mail: [e-mail]

North America: Tel: [phone] ext. 2258 · E-Mail: [e-mail]

Asia Pacific: Tel: [phone] · E-Mail: [e-mail]