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Centrifuge 5418 / 5418 R
Operating Manual
Copyright
©
2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior
permission of the copyright owner.
Trademarks
®
Eppendorf
Microtainer
and the Eppendorf logo are registered trademarks of Eppendorf AG.
®
is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.
Trademarks are not marked in all cases with ™ or
®
in this manual.
5401 900.022-07/102013
Table of contents
Centrifuge 5418 / 5418 R
English (EN)
Operating manualCentrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.
English (EN)Operating manual
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.1 Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.2 Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.4 Rotor FA-45-18-11. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.4.1 rcf display and calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3
3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.3 Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.3.1 Declaration concerning the ATEX directive (94/9/EC) . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.5.2 Incorrect handling of the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.5.4 Extreme strain on the centrifuging tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
3.5.5 Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.6 Safety instructions located on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.2 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.3 Installing the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.2 Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.2.1 Switching on the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.2.2 Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.2.3 Loading the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.2.4 Closing the rotor lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.2.5 Closing the centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Table of contents
Centrifuge 5418 / 5418 R
4
English (EN)
5.3 Cooling (only 5418 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.3.1 Temperature adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.3.2 Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.3.3 Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.3.4 FastTemp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.3.5 Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.4 Centrifuging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.4.1 Centrifugation with time setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.4.2 Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.4.3 Short-spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.4.4 Removing the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.5 Standby mode (only 5418) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.6 Special functions (only 5418 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.1 Prepare cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.2 Cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.2.1 Cleaning and disinfecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
6.2.2 Cleaning and disinfecting the rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
6.3 Additional service instructions for Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.4 Glass breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6.6 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
7.3 Emergency lid release. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
7.3.1 Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
7.3.2 Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
8.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
8.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
8.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
9 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
9.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
9.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
9.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
10 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
10.1 Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
10.2 Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
10.3 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
10.4 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
10.4.1 Fuses for Centrifuge 5418 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
10.4.2 Fuses for Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Operating instructions
Centrifuge 5418 / 5418 R
English (EN)
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time. Please also note the
operating instructions for the accessories, if applicable.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. You will find the current version on our webpage
www.eppendorf.com/worldwide.
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and danger levels:
5
1.2.1 Danger symbols
Biohazard Explosion
Electric shock Crushing
Hazard point Material damage
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
1.3 Symbols used
Symbol Meaning
Handling
1.
2.
• List
Text Name of fields in the software
Actions in the specified order
Useful information
Operating instructions
Centrifuge 5418 / 5418 R
6
English (EN)
1.4 Abbreviations used
PCR
Polymerase chain reaction
RZB/rcf
Relative centrifugal force – g-force in m/s
rpm
Revolutions per minute – in rpm
UV
Ultraviolet radiation
2
2 Product description
2.1 Main illustration
Abb. 2-1: Front and rear view of the Centrifuge 5418
1
2
3
4
5
Fig. 2-1: Front and rear view of the Centrifuge 5418
Abb. 2-2: Front and rear view of the Centrifuge 5418 R
Product description
Centrifuge 5418 / 5418 R
7
English (EN)
1
2
6
7
8
9
1
2
3
4
10
10
Fig. 2-2: Front and rear view of the Centrifuge 5418 R
1 Centrifuge lid
2 Monitoring glass
6 Name plate
7 Mains/power switch
Visual control for rotor stop or speed control
option using stroboscope
3 Display
Depiction of the current centrifuging parameters
8 Mains connection
and device settings.
1
2
5
6
9
7
8
Switch for switching the device on and off.
Switch position 0: The device is switched off.
Switch position I: The device is switched on.
Connection socket for the mains cable supplied.
4 Control panel
For operating the centrifuge.
5 Emergency lid release
9 Fuse holder
10 Condensation water tray (only Centrifuge 5418
R)
Product description
Centrifuge 5418 / 5418 R
8
English (EN)
2.2 Delivery package
2.2.1 Centrifuge 5418
Quantity Order no.
(International)
1 - - See chapter Ordering Information for corresponding
1 5425 351.003 022668188 2.5 A T (230 V), set of 2
or 5425 353.006 022668226 5 A T (120 V), UL, set of 2
1 5416 301.001 022634305 Standard
1- -
1 5401 900.022 5401900022 Languages: EN, DE, FR, ES, IT, PT
1 5401 900.030 5401900030 Languages: DA, FI, EL, NL, SV (230 V devices only)
Order no.
(North America)
Description
Centrifuge 5418
device version, equipment and order number
Fuse
Rotor key
Power supply device
Operating manual Centrifuge 5418/5418 R
2.2.2 Centrifuge 5418 R
Quantity Order no.
(International)
1 - - See chapter Ordering Information for corresponding
1 5425 351.003 022668188 2.5 A T (230 V), set of 2
or 5426 355.100 022668200 6.25 AT (100 V/120 V), set of 2
1 5416 301.001 022634305 Standard
1 5401 850.076 5401850076
1- -
1 5401 900.022 5401900022 Languages: EN, DE, FR, ES, IT, PT
1 5401 900.030 5401900030 Languages: DA, FI, EL, NL, SV (230 V devices only)
Order no.
(North America)
Description
Centrifuge 5418 R
device version, equipment and order number
Fuse
Rotor key
Tray for condensation water
Power supply device
Operating manual Centrifuge 5418/5418 R
Product description
Centrifuge 5418 / 5418 R
English (EN)
2.3 Features
The compact and easy-to-use Centrifuge 5418 / 5418 R has a capacity of 18 x 2 ml and reaches a maximum
of 16,873 x g / 14,000 rpm. The microcentrifuge is equipped with an aerosol-tight standard rotor for
centrifugation of the following tubes:
• Micro test tubes (0.2 to 2.0 ml)
• Microtainers (0.6 ml)
• Spin columns (1.5/2.0 ml)
The Centrifuge 5418 R has an additional temperature control function for centrifugation between 0°C and
+40°C. The fast temp function can be used to start a temperature control run without samples to adjust the
rotor chamber incl. rotor and adapters quickly to the set target temperature.
2.4 Rotor FA-45-18-11
Before using tubes, observe the manufacturer's recommended specifications on resistance to
centrifugation (max. g-force).
9
Max. capacity Max.
g-force (rcf)
or
Max. load
per rotor
(1)
bore
Notes
speed (rpm)
without
adapter
Acceleration/deceleration
(2)
time
Rotor
FA-45-18-11
18 micro test tubes of 1.5/
2.0 ml each or spin columns.
With adapters:
• 0.2 mL PCR tubes
16,873 x g /
3.75 g
14,000 rpm
5418: 16 s / 18 s
5418 R: 13 s / 13 s
• Aerosol-tight
rotor lid
(aluminum).
(3)
• 0.4 ml/0.5 ml micro test
tubes
• 0.6 mL Microtainers
(1) Maximum load per rotor bore for adapter + tube + contents.
(2) According to DIN 58 970 (device version: 230 V, 50 to 60 Hz).
(3) Aerosol tightness tested and certified by the Centre of Emergency Preparedness and Response, Health
Protection Agency, Porton Down (UK) (see certificates at the end of this operating manual).
Rotor code:
All Eppendorf® rotors are identified
using a simple, alphanumeric
format that represents the technical
specifications in a uniform series of
letters and numbers.
Fixed-angle
rotor
F
Aerosol-tight
version
Angle of
bores
Ø of
bores (mm)
A 45 30 11
Number of
bores
Swing-bucket
rotor
A
4 81
Number of
buckets
Ø of
buckets (mm)
10
Product description
Centrifuge 5418 / 5418 R
English (EN)
2.4.1 rcf display and calculation
Use the rpm/rcf key to switch the display of centrifugation speed between rcf and rpm.
Only 5418 R: For speeds ≤ 800 rpm only the lowest adjustable g-force (100 x g) is
displayed upon switching. The exact g-force (rcf) can be determined using the formula
given below.
Ensure that the g-force (rcf) displayed upon switching is standardized to suit the rotor
without an adapter. You can achieve the following maximum g-forces (rcf) at maximum
speed (rpm) when adapters are used:
Adapter Max. centrifugation
radius r
max
[cm]
Max. g-force (rcf)
Without adapter 7.7 16,873
for 0.2 mL PCR tubes 5.6 12,271
for 0.4 mL micro test tubes 7.7 16,873
for 0.5 mL micro test tubes 6.6 14,462
for 0.6 mL Microtainers 7.7 16,873
To determine the g-force (rcf) for a special adapter, you can perform a calculation according to DIN 58 970
with the following formula:
-5
rcf = 1.118 · 10
· n2 · r
max
n: Revolutions per minute (rpm)
r
: Max. centrifugation radius in cm
max
Example:
The 0.2 ml adapter has a maximum radius of 5.6 cm. At 5,000 rpm a maximum g-force of 1,565 x g is
reached.
Safety
Centrifuge 5418 / 5418 R
English (EN)
3 Safety
3.1 Intended use
The Centrifuge 5418 / 5418 R is intended exclusively for indoor use and for separating aqueous solutions
and suspensions of various densities in approved test tubes.
3.2 User profile
This device may only be operated by trained specialist staff. They must have carefully read the operating
manual and be familiar with the function of the device.
3.3 Application limits
3.3.1 Declaration concerning the ATEX directive (94/9/EC)
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DANGER! Risk of explosion.
Do not operate the device in areas where work is completed with explosive substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device for processing any substances which could generate an explosive
atmosphere.
Due to its design and the environmental conditions inside the device, the Centrifuge 5418 / 5418 R is not
suitable for use in a potentially explosive atmosphere.
The device only must be used in a safe environment, such as the open environment of a ventilated
laboratory or fume hood. The use of substances which could create a potentially explosive atmosphere is
not permitted. The final decision on the risks associated with the use of these types of substances is the
responsibility of the user.
3.3.2 Maximum service life for accessories
WARNING! Risk of injury from chemically or mechanically damaged accessories.
Even minor scratches and cracks can lead to serious internal material amage.
Protect all accessory parts from mechanical damage.
Inspect the accessories for damage before each use. Replace any damaged accessories.
Do not use rotors or rotor lids with signs of corrosion or mechanical damage (e.g.
deformations).
Do not use any accessories which are past their use-by date.
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Safety
Centrifuge 5418 / 5418 R
English (EN)
Accessories Maximum service from the first
commissioning onward
Rotor lid 3 years
Requirement: The "click" is still
audible when tightening the rotor lid
screw.
Plastic adapters 1 year
For the rotor described there is no limit on the service life as long as the following prerequisites are met:
• Proper use,
• Recommended maintenance
• Undamaged condition
The date of manufacture is stamped on the rotors in the format 03/10 (= March 2010). This is for
information only and does not have any reference to the service life.
To ensure aerosol tightness, the following applies:
• Replace aerosol-tight rotor lids and caps after 50 autoclaving cycles.
• Replace the seal of QuickLock rotor lids after 50 autoclaving cycles.
3.4 Information on product liability
In the following cases, the designated protection of the device may be compromised. Liability for any
resulting property damage or personal injury is then transferred to the operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
3.5 Warnings for intended use
Read the operating manual and observe the following general safety instructions before using the
Centrifuge 5418 / 5418 R.
3.5.1 Personal injury or damage to the equipment
WARNING! Electric shock due to damage to device or mains cable.
Only switch on the device if the device and mains cable are undamaged.
Only use devices that have been properly installed or repaired.
In case of danger, disconnect the device from the mains supply by pulling the power plug
from the device or the mains socket or, by using the isolating device intended for this
purpose (e.g., emergency stop switch in the laboratory).
Safety
Centrifuge 5418 / 5418 R
English (EN)
WARNING! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside the device
can be contacted by accident.
Do not remove the housing of the device.
Do not allow any liquids to penetrate the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who have been
specifically authorized by Eppendorf.
WARNING! Risk from incorrect supply voltage
Only connect the device to voltage sources that match the electrical requirements listed on
the name plate.
Only use sockets with a protective earth (PE) conductor and suitable power cable.
WARNING! Damages to health due to infectious liquids and pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the national regulations,
the biological security level of your laboratory, the material safety data sheets, and the
manufacturer's application notes.
Use aerosol tight sealing systems for the centrifugation of these substances.
When working with pathogenic germs belonging to a higher risk group, more than one
aerosol-tight bioseal must be used.
Wear personal protective equipment.
Consult the "Laboratory Biosafety Manual" (Source: World Health Organization,
Laboratory Biosafety Manual, as amended) for comprehensive regulations on the handling
of risk group II germs or biological materials).
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WARNING! Crushing of the fingers with the centrifuge lid.
When opening or closing the device lid, do not reach between the lid and device or into the
latching mechanism of the lid.
Always open the centrifuge lid completely to prevent it from falling.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or
accept any liability for damage resulting from the use of incorrect or non-recommended
accessories and spare parts, or from the improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
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Safety
Centrifuge 5418 / 5418 R
English (EN)
NOTICE! Damage to device due to spilled liquids.
1. Switch the device off.
2. Disconnect the device from the power supply.
3. Carefully clean the device and the accessories in accordance with the cleaning and
disinfection instructions in the operating manual.
4. If a different cleaning and disinfecting method is to be used, contact Eppendorf AG to
ensure that the intended method will not damage the device.
NOTICE! Damage to electronic components due to condensation.
Condensation can form inside the device after the device has been moved from a cool to a
warmer environment.
5418: Wait for at least 3 hours before connecting the device to the power supply.
Only 5418: Alternative: Let the device heat up for 30 minutes right before a brief transport.
5418 R: Wait for at least 4 hours before connecting the device to the power supply.
3.5.2 Incorrect handling of the centrifuge
NOTICE! Damage from knocking against or moving the device during operation.
If the rotor bangs against the rotor chamber wall, it will cause considerable damage to the
device and rotor.
Do not move or knock against the device during operation.
3.5.3 Incorrect handling of the rotors
WARNING! Risk of injury from improperly attached rotors and rotor lids.
Only centrifuge with rotor and rotor lid firmly tightened.
If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be
properly secured. Immediately press the start/stop key to stop centrifuging.
CAUTION! Risk of injury due to asymmetric loading of a rotor.
Load rotors symmetrically with identical tubes.
Only load adapters with suitable tubes.
Always use the same type of tubes (weight, material/density and volume).
Check symmetric loading by balancing the adapters and tubes used with scales.
CAUTION! Risk of injury from overloaded rotor.
The Centrifuge 5418 / 5418 R is designed for the centrifugation of centrifugation material with
a max. density of 1.2 g/mL at maximum speed and volume.
Please note the information on each rotor on the maximum load (adapter, tube and
contents) per rotor bore and do not exceed it.
NOTICE! Damage to rotors from aggressive chemicals.
Rotors are high-quality components which withstand extreme stresses. This stability can be
impaired by aggressive chemicals.
Avoid the use of aggressive chemicals, including strong and weak alkali, strong acids,
solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons,
concentrated saline solutions and phenol.
Due to the manufacturing process, color variations may occur on rotors marked "coated".
These color variations do not effect service life or resistance to chemicals.
3.5.4 Extreme strain on the centrifuging tubes
CAUTION! Risk of injury from overloaded tubes.
Note the loading limits specified by the tube manufacturer.
Only use tubes which are approved by the manufacturer for the required rcf.
Safety
Centrifuge 5418 / 5418 R
English (EN)
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NOTICE! Risk from damaged tubes.
Damaged tubes must not be used, as this could cause further damage to the device and the
accessories and loss of the samples.
Before use, visually check all of the tubes for damage.
NOTICE! Risk from open tube lids.
Open tube lids can break off during centrifugation and damage both the rotor and the
centrifuge.
Carefully seal all tube lids before centrifuging.
NOTICE! Hazard to plastic tubes from organic solvents.
The density of plastic tubes is reduced when organic solvents (e.g., phenol, chloroform) are
used, i.e. the tubes could become damaged.
Note the manufacturer’s information on the chemical resistance of the tubes.
NOTICE! Sample tubes heat up.
In uncooled centrifuges, the temperature in the rotor chamber, rotor and sample can increase
to above 40 °C, based on the run time, g-force (rcf)/speed and ambient temperature.
Note that this can reduce the centrifugation resistance of the sample tubes.
Please note the temperature resistance of the samples.
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