Eppendorf 5418 R User Manual [en, es]

www.eppendorf.com/myeppendorf

Centrifuge 5418 / 5418 R

Operating Manual Manual de instrucciones

2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior

permission of the copyright owner.

Trademarks

Eppendorf

Microtainer

and the Eppendorf logo are registered trademarks of Eppendorf AG.

is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.

Trademarks are not marked in all cases with ™ or

in this manual.

Operating manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Manual de instrucciones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

5401 900.022-07/102013

Table of contents

Centrifuge 5418 / 5418 R

English (EN)

Operating manualCentrifuge 5418 / 5418 Rseeon p.Fig.Tab.p.

English (EN)Operating manual

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.1 Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.2.1 Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.2.2 Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.4 Rotor FA-45-18-11. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.4.1 rcf display and calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3 Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3.1 Declaration concerning the ATEX directive (94/9/EC) . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.5.2 Incorrect handling of the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.5.4 Extreme strain on the centrifuging tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.5.5 Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.6 Safety instructions located on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

4.2 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

4.3 Installing the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

5 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.1 Overview of operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.2 Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.2.1 Switching on the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.2.2 Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.2.3 Loading the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.2.4 Closing the rotor lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

5.2.5 Closing the centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Table of contents

Centrifuge 5418 / 5418 R

English (EN)

5.3 Cooling (only 5418 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5.3.1 Temperature adjustment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5.3.2 Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5.3.3 Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5.3.4 FastTemp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.3.5 Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.4 Centrifuging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.4.1 Centrifugation with time setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.4.2 Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

5.4.3 Short-spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

5.4.4 Removing the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.5 Standby mode (only 5418) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.6 Special functions (only 5418 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

6.1 Prepare cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

6.2 Cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

6.2.1 Cleaning and disinfecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

6.2.2 Cleaning and disinfecting the rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

6.3 Additional service instructions for Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

6.4 Glass breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

6.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

6.6 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

7.3 Emergency lid release. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

7.3.1 Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

7.3.2 Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

9 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

9.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

9.4 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

10.1 Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

10.2 Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

10.3 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

10.4 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

10.4.1 Fuses for Centrifuge 5418. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

10.4.2 Fuses for Centrifuge 5418 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Operating instructions

Centrifuge 5418 / 5418 R

English (EN)

1 Operating instructions

1.1 Using this manual

 Read this operating manual completely before using the device for the first time. Please also note the

operating instructions for the accessories, if applicable.

 This operating manual is part of the product. Thus, it must always be easily accessible.  Enclose this operating manual when transferring the device to third parties.  If this manual is lost, please request another one. You will find the current version on our webpage

www.eppendorf.com/worldwide.

1.2 Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1 Danger symbols

Biohazard Explosion

Electric shock Crushing

Hazard point Material damage

1.2.2 Danger levels

DANGER Will lead to severe injuries or death. WARNING May lead to severe injuries or death. CAUTION May lead to light to moderate injuries. NOTICE May lead to material damage.

1.3 Symbols used

Symbol Meaning

 Handling

• List

Text Name of fields in the software

Actions in the specified order

Useful information

Operating instructions

Centrifuge 5418 / 5418 R

English (EN)

1.4 Abbreviations used

PCR

Polymerase chain reaction

RZB/rcf

Relative centrifugal force – g-force in m/s

rpm

Revolutions per minute – in rpm

Ultraviolet radiation

2 Product description

2.1 Main illustration

Abb. 2-1: Front and rear view of the Centrifuge 5418

1 2

3 4

Fig. 2-1: Front and rear view of the Centrifuge 5418

Abb. 2-2: Front and rear view of the Centrifuge 5418 R

Product description

Centrifuge 5418 / 5418 R

English (EN)

1 2

7 8 9

1 2

3 4

Fig. 2-2: Front and rear view of the Centrifuge 5418 R

1 Centrifuge lid

2 Monitoring glass

6 Name plate

7 Mains/power switch

Visual control for rotor stop or speed control option using stroboscope

3 Display

Depiction of the current centrifuging parameters

8 Mains connection

and device settings.

1 2

6 9

7 8

Switch for switching the device on and off. Switch position 0: The device is switched off. Switch position I: The device is switched on.

Connection socket for the mains cable supplied.

4 Control panel

For operating the centrifuge.

5 Emergency lid release

9 Fuse holder

10 Condensation water tray (only Centrifuge 5418

Product description

Centrifuge 5418 / 5418 R

English (EN)

2.2 Delivery package

2.2.1 Centrifuge 5418

Quantity Order no.

(International)

1 - - See chapter Ordering Information for corresponding

1 5425 351.003 022668188 2.5 A T (230 V), set of 2 or 5425 353.006 022668226 5 A T (120 V), UL, set of 2

1 5416 301.001 022634305 Standard

1- -

1 5401 900.022 5401900022 Languages: EN, DE, FR, ES, IT, PT 1 5401 900.030 5401900030 Languages: DA, FI, EL, NL, SV (230 V devices only)

Order no. (North America)

Description

Centrifuge 5418

device version, equipment and order number

Fuse

Rotor key

Power supply device

Operating manual Centrifuge 5418/5418 R

2.2.2 Centrifuge 5418 R

Quantity Order no.

(International)

1 - - See chapter Ordering Information for corresponding

1 5425 351.003 022668188 2.5 A T (230 V), set of 2 or 5426 355.100 022668200 6.25 AT (100 V/120 V), set of 2

1 5416 301.001 022634305 Standard

1 5401 850.076 5401850076

1- -

1 5401 900.022 5401900022 Languages: EN, DE, FR, ES, IT, PT 1 5401 900.030 5401900030 Languages: DA, FI, EL, NL, SV (230 V devices only)

Order no. (North America)

Description

Centrifuge 5418 R

device version, equipment and order number

Fuse

Rotor key

Tray for condensation water

Power supply device

Operating manual Centrifuge 5418/5418 R

Product description

Centrifuge 5418 / 5418 R

English (EN)

2.3 Features

The compact and easy-to-use Centrifuge 5418 / 5418 R has a capacity of 18 x 2 ml and reaches a maximum of 16,873 x g / 14,000 rpm. The microcentrifuge is equipped with an aerosol-tight standard rotor for centrifugation of the following tubes:

• Micro test tubes (0.2 to 2.0 ml)

• Microtainers (0.6 ml)

• Spin columns (1.5/2.0 ml)

The Centrifuge 5418 R has an additional temperature control function for centrifugation between 0°C and +40°C. The fast temp function can be used to start a temperature control run without samples to adjust the rotor chamber incl. rotor and adapters quickly to the set target temperature.

2.4 Rotor FA-45-18-11

Before using tubes, observe the manufacturer's recommended specifications on resistance to centrifugation (max. g-force).

Max. capacity Max.

g-force (rcf) or

Max. load per rotor

(1)

bore

Notes

speed (rpm) without adapter

Acceleration/deceleration

(2)

time

Rotor FA-45-18-11

18 micro test tubes of 1.5/

2.0 ml each or spin columns. With adapters:

• 0.2 mL PCR tubes

16,873 x g /

3.75 g

14,000 rpm 5418: 16 s / 18 s

5418 R: 13 s / 13 s

• Aerosol-tight rotor lid (aluminum).

(3)

• 0.4 ml/0.5 ml micro test tubes

• 0.6 mL Microtainers

(1) Maximum load per rotor bore for adapter + tube + contents.

(2) According to DIN 58 970 (device version: 230 V, 50 to 60 Hz).

(3) Aerosol tightness tested and certified by the Centre of Emergency Preparedness and Response, Health Protection Agency, Porton Down (UK) (see certificates at the end of this operating manual).

Rotor code:

All Eppendorf® rotors are identiﬁed using a simple, alphanumeric format that represents the technical speciﬁcations in a uniform series of letters and numbers.

Fixed-angle

rotor

Aerosol-tight

version

Angle of

bores

Ø of

bores (mm)

A 45 30 11

Number of

bores

Swing-bucket

rotor

4 81

Number of

buckets

Ø of

buckets (mm)

Product description

Centrifuge 5418 / 5418 R English (EN)

2.4.1 rcf display and calculation

Use the rpm/rcf key to switch the display of centrifugation speed between rcf and rpm. Only 5418 R: For speeds ≤ 800 rpm only the lowest adjustable g-force (100 x g) is displayed upon switching. The exact g-force (rcf) can be determined using the formula given below. Ensure that the g-force (rcf) displayed upon switching is standardized to suit the rotor without an adapter. You can achieve the following maximum g-forces (rcf) at maximum speed (rpm) when adapters are used:

Adapter Max. centrifugation

radius r

max

[cm]

Max. g-force (rcf)

Without adapter 7.7 16,873 for 0.2 mL PCR tubes 5.6 12,271 for 0.4 mL micro test tubes 7.7 16,873 for 0.5 mL micro test tubes 6.6 14,462 for 0.6 mL Microtainers 7.7 16,873

To determine the g-force (rcf) for a special adapter, you can perform a calculation according to DIN 58 970 with the following formula:

-5

rcf = 1.118 · 10

· n2 · r

max

n: Revolutions per minute (rpm)

: Max. centrifugation radius in cm

max

Example:

The 0.2 ml adapter has a maximum radius of 5.6 cm. At 5,000 rpm a maximum g-force of 1,565 x g is reached.

Safety

Centrifuge 5418 / 5418 R

English (EN)

3 Safety

3.1 Intended use

The Centrifuge 5418 / 5418 R is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes.

3.2 User profile

This device may only be operated by trained specialist staff. They must have carefully read the operating manual and be familiar with the function of the device.

3.3 Application limits

3.3.1 Declaration concerning the ATEX directive (94/9/EC)

DANGER! Risk of explosion.

 Do not operate the device in areas where work is completed with explosive substances.  Do not use this device to process any explosive or highly reactive substances.  Do not use this device for processing any substances which could generate an explosive

atmosphere.

Due to its design and the environmental conditions inside the device, the Centrifuge 5418 / 5418 R is not suitable for use in a potentially explosive atmosphere.

The device only must be used in a safe environment, such as the open environment of a ventilated laboratory or fume hood. The use of substances which could create a potentially explosive atmosphere is not permitted. The final decision on the risks associated with the use of these types of substances is the responsibility of the user.

3.3.2 Maximum service life for accessories

WARNING! Risk of injury from chemically or mechanically damaged accessories.

Even minor scratches and cracks can lead to serious internal material amage.

 Protect all accessory parts from mechanical damage.  Inspect the accessories for damage before each use. Replace any damaged accessories.  Do not use rotors or rotor lids with signs of corrosion or mechanical damage (e.g.

deformations).

 Do not use any accessories which are past their use-by date.

Safety

Centrifuge 5418 / 5418 R English (EN)

Accessories Maximum service from the first

commissioning onward

Rotor lid 3 years

Requirement: The "click" is still audible when tightening the rotor lid screw.

Plastic adapters 1 year

For the rotor described there is no limit on the service life as long as the following prerequisites are met:

• Proper use,

• Recommended maintenance

• Undamaged condition

The date of manufacture is stamped on the rotors in the format 03/10 (= March 2010). This is for information only and does not have any reference to the service life.

To ensure aerosol tightness, the following applies:

• Replace aerosol-tight rotor lids and caps after 50 autoclaving cycles.

• Replace the seal of QuickLock rotor lids after 50 autoclaving cycles.

3.4 Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

• The device is not used in accordance with the operating manual.

• The device is used outside of its intended use.

• The device is used with accessories or consumables which are not recommended by Eppendorf.

• The device is maintained or repaired by people not authorized by Eppendorf.

• The user makes unauthorized changes to the device.

3.5 Warnings for intended use

Read the operating manual and observe the following general safety instructions before using the Centrifuge 5418 / 5418 R.

3.5.1 Personal injury or damage to the equipment

WARNING! Electric shock due to damage to device or mains cable.

 Only switch on the device if the device and mains cable are undamaged.  Only use devices that have been properly installed or repaired.  In case of danger, disconnect the device from the mains supply by pulling the power plug

from the device or the mains socket or, by using the isolating device intended for this purpose (e.g., emergency stop switch in the laboratory).

Safety

Centrifuge 5418 / 5418 R

English (EN)

WARNING! Lethal voltages inside the device.

 Ensure that the housing is always closed and undamaged so that no parts inside the device

can be contacted by accident.

 Do not remove the housing of the device.  Do not allow any liquids to penetrate the inside of the housing.  Do not allow the device to be opened by anyone except service personnel who have been

specifically authorized by Eppendorf.

WARNING! Risk from incorrect supply voltage

 Only connect the device to voltage sources that match the electrical requirements listed on

the name plate.

 Only use sockets with a protective earth (PE) conductor and suitable power cable.

WARNING! Damages to health due to infectious liquids and pathogenic germs.

 When handling infectious liquids and pathogenic germs, observe the national regulations,

the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes.

 Use aerosol tight sealing systems for the centrifugation of these substances.  When working with pathogenic germs belonging to a higher risk group, more than one

aerosol-tight bioseal must be used.

 Wear personal protective equipment.  Consult the "Laboratory Biosafety Manual" (Source: World Health Organization,

Laboratory Biosafety Manual, as amended) for comprehensive regulations on the handling of risk group II germs or biological materials).

WARNING! Crushing of the fingers with the centrifuge lid.

 When opening or closing the device lid, do not reach between the lid and device or into the

latching mechanism of the lid.

 Always open the centrifuge lid completely to prevent it from falling.

CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts, or from the improper use of such equipment.

 Only use accessories and original spare parts recommended by Eppendorf.

Safety

Centrifuge 5418 / 5418 R English (EN)

NOTICE! Damage to device due to spilled liquids.

1. Switch the device off.

2. Disconnect the device from the power supply.

3. Carefully clean the device and the accessories in accordance with the cleaning and disinfection instructions in the operating manual.

4. If a different cleaning and disinfecting method is to be used, contact Eppendorf AG to ensure that the intended method will not damage the device.

NOTICE! Damage to electronic components due to condensation.

Condensation can form inside the device after the device has been moved from a cool to a warmer environment.

 5418: Wait for at least 3 hours before connecting the device to the power supply.  Only 5418: Alternative: Let the device heat up for 30 minutes right before a brief transport.  5418 R: Wait for at least 4 hours before connecting the device to the power supply.

3.5.2 Incorrect handling of the centrifuge

NOTICE! Damage from knocking against or moving the device during operation.

If the rotor bangs against the rotor chamber wall, it will cause considerable damage to the device and rotor.

 Do not move or knock against the device during operation.

3.5.3 Incorrect handling of the rotors

WARNING! Risk of injury from improperly attached rotors and rotor lids.

 Only centrifuge with rotor and rotor lid firmly tightened.  If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be

properly secured. Immediately press the start/stop key to stop centrifuging.

CAUTION! Risk of injury due to asymmetric loading of a rotor.

 Load rotors symmetrically with identical tubes.  Only load adapters with suitable tubes.  Always use the same type of tubes (weight, material/density and volume).  Check symmetric loading by balancing the adapters and tubes used with scales.

CAUTION! Risk of injury from overloaded rotor.

The Centrifuge 5418 / 5418 R is designed for the centrifugation of centrifugation material with a max. density of 1.2 g/mL at maximum speed and volume.

 Please note the information on each rotor on the maximum load (adapter, tube and

contents) per rotor bore and do not exceed it.

NOTICE! Damage to rotors from aggressive chemicals.

Rotors are high-quality components which withstand extreme stresses. This stability can be impaired by aggressive chemicals.

 Avoid the use of aggressive chemicals, including strong and weak alkali, strong acids,

solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons, concentrated saline solutions and phenol.

 Due to the manufacturing process, color variations may occur on rotors marked "coated".

These color variations do not effect service life or resistance to chemicals.

3.5.4 Extreme strain on the centrifuging tubes

CAUTION! Risk of injury from overloaded tubes.

 Note the loading limits specified by the tube manufacturer.  Only use tubes which are approved by the manufacturer for the required rcf.

Safety

Centrifuge 5418 / 5418 R

English (EN)

NOTICE! Risk from damaged tubes.

Damaged tubes must not be used, as this could cause further damage to the device and the accessories and loss of the samples.

 Before use, visually check all of the tubes for damage.

NOTICE! Risk from open tube lids.

Open tube lids can break off during centrifugation and damage both the rotor and the centrifuge.

 Carefully seal all tube lids before centrifuging.

NOTICE! Hazard to plastic tubes from organic solvents.

The density of plastic tubes is reduced when organic solvents (e.g., phenol, chloroform) are used, i.e. the tubes could become damaged.

 Note the manufacturer’s information on the chemical resistance of the tubes.

NOTICE! Sample tubes heat up.

In uncooled centrifuges, the temperature in the rotor chamber, rotor and sample can increase to above 40 °C, based on the run time, g-force (rcf)/speed and ambient temperature.

 Note that this can reduce the centrifugation resistance of the sample tubes.  Please note the temperature resistance of the samples.

Safety

Centrifuge 5418 / 5418 R English (EN)

3.5.5 Aerosol-tight centrifugation

WARNING! Risk to health due to limited aerosol tightness with incorrect rotor/rotor lid combination.

Aerosol-tight centrifugation is guaranteed only if the rotors and rotor lids intended for this purpose are used. For fixed-angle rotors the labeling always begins with FA, swing-bucket rotors are labeled with AT (aerosol tight). The aerosol-tight rotors and rotor lids of this centrifuge are additionally marked with a red ring on the rotor and a red rotor lid screw.

 For aerosol-tight centrifugation, always simultaneously use rotors and rotor lids which are

marked as aerosol-tight in the centrifuge intended for the corresponding purpose. The details specifying in which centrifuge you may use the aerosol-tight rotors and rotor lids can be found on the rotor and, beginning from production date of October 2003, on the upper side of the rotor lid.

 Only use aerosol-tight rotor lids in combination with rotors which are marked on the rotor

lid.

WARNING! Health hazard from limited aerosol-tightness due to incorrect use.

Autoclaving, mechanical stresses and contamination by chemicals or other aggressive solvents can impair the aerosol-tightness of the rotors and rotor lid.

 Check the integrity of the seals of the aerosol-tight rotor lids or caps before each use.  Only use aerosol-tight rotor lids or caps if the seals are undamaged and clean.  Thinly brush the threads of the rotor lid screw with pivot grease (order no. Int.

5810 350.050, North America 022634330). Do not apply the pivot grease to the seals.

 Replace aerosol-tight rotor lids and caps after 50autoclaving cycles.  For QuickLock rotor lids, the seal must be replaced after 50 autoclaving cycles.  Never store aerosol-tight rotors or buckets closed.

3.6 Safety instructions located on the device

Display Meaning Location

Follow the instructions in the operating manual. 5418: Rear of the device

CAUTION

Always tighten the rotor using the supplied rotor key.

CAUTION

Close all tubes and use a rotor lid.

5418 R: Right side of the device

Top of device, below the centrifuge lid

4 Installation

4.1 Selecting the location

NOTICE! If an error occurs, the objects in the immediate proximity of the device will be damaged.

 In accordance with recommendations in EN 61010-2-020, leave a safety clearance of 30

cm around the device during operation.

 Please remove all materials and objects from this area.

NOTICE! Damage from overheating.

 Do not install the device near to any heat sources (e.g., heating, drying cabinet).  Do not expose the device to direct sunlight.  Ensure unobstructed air circulation. Keep free a clearance of at least 33 cm around all

ventilation grilles.

Installation

Centrifuge 5418 / 5418 R

English (EN)

Select the location for the device according to the following criteria:

• Suitable power connection as per the name plate (230 V/120 V/100 V).

• Stable, horizontal and resonance-free lab bench. Weight of the device: 7.7 kg (5418) or 22 kg (5418 R).

• A well ventilated environment which is protected from direct sunlight to prevent the device from heating up more.

4.2 Preparing installation

Prerequisites The weight of the Centrifuge 5418 is 7.7 kg or the Centrifuge 5418 R 22 kg. For unpacking and installing

the Centrifuge 5418 R, you require the assistance of another person.

Perform the following steps in the sequence described.

1. Open the packaging board.

2. Remove the accessories.

3. Lift the Centrifuge 5418 / 5418 R out of the carton. Lift the Centrifuge 5418 R out of the carton with the help of another person.

4. Remove the front and back transport protection pads.

5. Place the device on a suitable lab bench.

6. Remove the plastic sleeve.

Installation

Centrifuge 5418 / 5418 R English (EN)

4.3 Installing the instrument

NOTICE! Damage to electronic components due to condensation.

Condensation can form inside the device after the device has been moved from a cool to a warmer environment.

NOTICE! Centrifuge 5418 R: compressor damage after improper transport.

 Only switch on the centrifuge 4 hours after installation.

Perform the following steps in the sequence described.

1. Let the device warm up to ambient temperature.

2. Check that the mains voltage and frequency match the requirements on the device type plate.

3. Connect the centrifuge to the mains and switch it on using the mains/power switch.

• Display is active.

• Lid opens automatically

4. Loosen and remove the rotor using the supplied rotor key.

5. Remove the transport protection pad from the motor shaft.

6. Use the details included in the scope of delivery to check that the delivery is complete.

7. Check all parts for any transport damage. Contact your dealer if any damage is found.

8. Only 5418 R: Insert the condensation water tray at the side of the device into the holder provided.

Retain the packaging material and the transport protection device for subsequent transport or storage. See also the instructions relating to transport.

Operation

Centrifuge 5418 / 5418 R

English (EN)

5 Operation

5.1 Overview of operating controls

Familiarize yourself with the operating controls and the display before using the Centrifuge 5418 / 5418 R for the first time.

Abb. 5-1: Control panel of Centrifuge 5418 and Centrifuge 5418 R.

5418

4 5

4 (9)

5418 R

Fig. 5-1: Control panel of Centrifuge 5418 and Centrifuge 5418 R.

1 Switch the displayed speed of centrifugation

(rpm/rcf)

2 Display

3 Release lid

4 Set centrifugation time

6 Adjust temperature (only 5418 R)

7 Start the temperature control run fast temp

(only 5418 R)

8 Short-spin centrifugation

9 Start and stop centrifugation

Only 5418 R: Press the time dial to start and stop

centrifugation.

2 5 (1)

5 Set speed of centrifugation

Only 5418 R: Press the speed dial to switch the

displayed centrifugation speed (rpm/rcf).

Operation

Centrifuge 5418 / 5418 R English (EN)

Abb. 5-2: Display of Centrifuge 5418 and Centrifuge 5418 R.

1 2

5418

5418 R

Fig. 5-2: Display of Centrifuge 5418 and Centrifuge 5418 R.

1 Centrifugation time

2 g-force (rcf) / speed (rpm)

5418 R: Set value x 1000

3 Status of centrifuge

: Centrifuge lid unlocked. : Centrifuge lid locked. (Flashing): Centrifuging in progress.

4 At set rpm (only 5418 R)

: Start of run time when reaching 95% of the

preset g-force (rcf) / speed (rpm).

: Immediate start of run time.

5 Temperature (only 5418 R)

6 Status of the key lock (only 5418 R)

: Centrifuging parameters cannot be modified

unintentionally.

: No key lock.

7 Status of centrifugation speed display

rcf: g-force (relative centrifugal force, RCF) rpm: speed (revolutions per minute)

8 Status of the loudspeaker (only 5418 R)

: Switched on.

No symbol: Switched off.

5.2 Preparing for centrifugation

5.2.1 Switching on the centrifuge

1. Switch on the centrifuge using the mains switch. The centrifuge lid opens automatically after switching on using the mains switch.

2. Open the closed centrifuge lid by pressing the open key. The parameter settings of the last run are displayed.

5.2.2 Inserting the rotor

NOTICE! If handled incorrectly, the rotor can fall over.

The rotor lid screw may become loose if it is used to retain the rotor.

 Always grasp the rotor with both hands for holding or transport.

1. Place the rotor on the motor shaft.

2. Insert the delivered rotor key in the rotor nut.

3. Turn rotor key clockwise until the rotor nut is firmly tightened.

Centrifuge 5418 / 5418 R

5.2.3 Loading the rotor

CAUTION! Risk of injury due to asymmetric loading of a rotor.

CAUTION! Risk from damaged or overloaded tubes.

 When loading the rotor, observe the safety precautions on dangers as a result of

overloaded or damaged tubes (see Warnings for intended use on p. 12).

To load the rotor, proceed as follows:

1. Check the maximum load (adapter, tube and contents) per rotor bore. The maximum load is 3.75 g per rotor bore. This information can also be found on the rotor.

Operation

English (EN)

2. Load rotors and adapters only with the tubes intended for them.

3. Insert tubes opposite each other in pairs into the rotor bores. For symmetric loading, tubes that face each other must be the same type and have the same filling quantity.

To keep the weight differences between the filled tubes low, we recommend taring with a balance. This is gentle on the drive and reduces the operating noises.

5.2.4 Closing the rotor lid

With the rotor FA-45-18-11 centrifugation is also possible without a rotor lid.

Please also note:

• The tube lids must be closed securely.

• Without the rotor lid, the rotor is not aerosol-tight.

• The centrifugation is slightly louder.

• Spin columns must always be centrifuged with a rotor lid.

Operation

Centrifuge 5418 / 5418 R English (EN)

1. Check to see if the outer sealing ring is correctly positioned in the groove.

2. Fit the rotor lid vertically onto the rotor.

3. Lock the rotor by turning the red rotor lid screw clockwise beyond an audible click until it can be turned no further.

The rotor is correctly locked after the audible "click" is heard!

5.2.5 Closing the centrifuge lid

WARNING! Crushing of the fingers with the centrifuge lid.

 When opening or closing the device lid, do not reach between the lid and device or into the

latching mechanism of the lid.

 Always open the centrifuge lid completely to prevent it from falling.

1. Check correct attachment of rotor and rotor lid.

2. Push down the centrifuge lid until the lid latch engages and the lid is automatically closed.

The centrifuge will close automatically.

The display shows the symbol.

Only 5418: The open key lights blue.

5.3 Cooling (only 5418 R)

5.3.1 Temperature adjustment

 Set the temperature using the temp arrow keys from 0°C to +40°C.

5.3.2 Temperature display

If the rotor is stopped: Set temperature During centrifugation: Actual temperature

5.3.3 Temperature monitoring

After the set temperature has been reached the centrifuge responds as follows to temperature fluctuations during centrifugation:

Deviation from the target value Action

ΔT > 3°C Temperature display flashes. ΔT > 5°C Periodic warning tone and display Error 18.

Centrifugation is stopped automatically.

Operation

Centrifuge 5418 / 5418 R

English (EN)

5.3.4 FastTemp

This function can be used to start a temperature control run directly without samples with a rotor and temperature-specific speed in order to quickly adjust the rotor chamber, incl. rotor and adapters, up to the set target temperature.

Prerequisites

• The centrifuge is switched on.

• Rotor and rotor lid are correctly mounted.

• The centrifuge lid is closed.

• The temperature and g-force (rcf) or speed (rpm) are set for the subsequent centrifugation (see Centrifuging on p. 24).

1. Press the fast temp key. The display shows FT as well as the current temperature and g-force (rcf) or speed (rpm).

The cooling time from room temperature (≈23 °C) to 4 °C takes Approx. 16 min. The temperature control cycle ends automatically when the target temperature has been reached. A periodic signal tone sounds.

2. Press the start/stop key to end the temperature control run early.

After the set temperature has been reached and the temperature control run is complete, the centrifuge keeps the rotor chamber, with the centrifuge lid closed, at the set temperature, if the temperature is below the ambient temperature. Irrespective of the target temperature, however, this continuous cooling does not go below 4°C to prevent the rotor chamber from freezing.

The centrifuge stops the run automatically when the rotor has reached the set temperature. Therefore, there may be a delay between the display of the achieved set temperature and the automatic end of the temperature control run.

5.3.5 Continuous cooling

When the rotor is stopped the rotor chamber is kept at the target temperature when the following prerequisites are met:

• The centrifuge is switched on.

• The centrifuge lid is closed.

• The target temperature is below the ambient temperature.

During continuous cooling the following applies:

• The target temperature is displayed.

• Irrespective of the target temperature, continuous cooling does not go below 4°C to prevent the rotor chamber and the samples from freezing and increased condensation in the device.

• The temperature adjustment takes longer because the rotor is not rotating.

Open the centrifuge lid to end continuous cooling.

If the centrifuge is not used for more than 8 hours, the continuous cooling is switched off automatically. This protects against ice formation in the rotor chamber and the tubes, and against increased condensation in the device.

The display shows the actual temperature of the rotor chamber.

With fast temp you can quickly reach the desired temperature again (see p. 23).

Operation

Centrifuge 5418 / 5418 R English (EN)

5.4 Centrifuging

CAUTION! Risk from incorrectly-loaded rotors and damaged/overloaded tubes!

 Before commencing centrifugation, follow the safety instructions relating to risks from

asymmetrically loaded and/or overloaded rotors and from overloaded, damaged and/or open tubes (see Warnings for intended use on p. 12).

WARNING! Risk of injury from improperly attached rotors and rotor lids.

 Only centrifuge with rotor and rotor lid firmly tightened.  If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be

properly secured. Immediately press the start/stop key to stop centrifuging.

Before using the Centrifuge 5418 / 5418 R for the first time, familiarize yourself with the operating controls and the display (see Overview of operating controls on p. 19).

Each of the centrifuging variants described here must be preceded by the preparation described above (see Preparing for centrifugation on p. 20).

Only 5418 R: Please also note the instructions on cooling (see p. 22).

5.4.1 Centrifugation with time setting

Perform the following steps in the sequence described.

1. Use time to set the run time.

2. Only 5418 R: Use temp to set the temperature.

3. Use speed to set the g-force (rcf) or speed (rpm).

4. Press start/stop to start centrifuging.

During centrifugation:

• In the display flashes while the rotor is running.

• Only 5418 R: The current temperature will be displayed.

• The fast temp (only 5418 R), open and short keys are blocked during centrifugation.

• During the run you can modify the total run time, the temperature (only 5418 R), the speed and the

rpm/rcf display. Only 5418 R: To change the centrifugation parameters, press the short key first. The values flash in the display during the change. The new parameters are adopted immediately. When the time is changed during a run, the time which has already elapsed is taken into account. Please note that the shortest new total run time that can be set is the elapsed time plus 2 minutes.

• You can also terminate the centrifugation before the set run time has elapsed by pressing the start/

stop key.

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