Bio-Rad C-P Lift Membranes User Manual

C/P Lift®Membranes

Instruction Manual

Catalog Numbers

162-0162
162-0163

Table of Contents

Section 1 Introduction.............................................1

Section 2 Colony Screening ....................................1

2.1 Direct Lifts................................................1

2.2 Replica Lifts..............................................3

Section 3 Plaque Screening.....................................5

Section 4 Hybridization Protocol...........................5

4.1 Prehybridization........................................5

4.2 Hybridization ............................................6

4.3 Washes......................................................7

Section 1
Introduction

C/P Lift membranes were developed for colony and
plaque screening. When handling the membranes,
always wear gloves or use forceps. After blotting, do not
allow wet membranes to come in contact with each
other. Contact may result in the transfer of blotted
nucleic acids from one membrane to the other.

Section 2
Colony Screening

2.1 Direct Lifts

1. Plate cells containing recombinant plasmids onto the

appropriate selective agar. Incubate plated cells until
the colonies are 1-2 mm in diameter.

2. Place a circle of C/P Lift membrane onto the surface

of the plate as follows. Hold the membrane at
opposite edges with forceps, bending it slightly into
a ‘U’-shape, and then lower the membrane until the
fold makes contact with the center of the plate.

1