Bio-Rad Biotinylated Standards User Manual

Ordering Information

Catalog
Number Product Description

161-0319 Biotinylated SDS-PAGE Standards, Broad Range,250 µl
161-0306 Biotinylated SDS-PAGE Standards, Low Range, 250 µl
161-0311 Biotinylated SDS-PAGE Standards, High Range, 250 µl
161-0322 Biotinylated SDS-PAGE Standards Kit, Broad Range, AP*
161-0321 Biotinylated SDS-PAGE Standards Kit, Broad Range, HRP*
161-0307 Biotinylated SDS-PAGE Standards Kit, Low Range, HRP*
161-0308 Biotinylated SDS-PAGE Standards Kit, Low Range, AP*
161-0312 Biotinylated SDS-PAGE Standards Kit, High Range, HRP*
161-0313 Biotinylated SDS-PAGE Standards Kit, High Range, AP*
170-6528 Avidin-HRP, 2 ml
170-6533 Avidin-AP, 1 ml
170-6431 Horseradish Peroxidase Conjugate Substrate Kit
170-6432 Alkaline Phosphatase Conjugate Substrate Kit
* Each kit contains 250 µl Biotinylated Standards, 1 ml

Avidin-AP or 2 ml Avidin-HRP, and complete instructions.

Biotinylated SDS-PAGE

Standards, Low, High,

and Broad Range

Catalog Numbers

161-0306
161-031 1
161-0319

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547

LIT395 Rev D

Biotinylated SDS-PAGE
Standards, Low , High,
and Broad Range

Bio-Rad’s Biotinylated SDS-PAGE Standards are
a mixture of biotinylated proteins that can be used for
accurate molecular weight determinations of immune
detected proteins. The standards have been blended to
give equal intensities when detected with avidin-HRP
and HRP Color Development Reagent.

The Biotinylated SDS-PAGE Standards and the
sample proteins are run on an SDS polyacrylamide gel
and electrophoretically transferred to nitrocellulose,
PVDF, or Zeta-Probe®membranes. The protein sam­ples and standards are exposed to the same reagents
during detection. This prevents distortions that may
occur if the standards lane is treated separately.

The molecular weights of the standards are not sig­nificantly altered by biotinylation. The consistent
molecular weights of the standards give accurate
molecular weight determinations every time without
increasing the number of steps or length of time of
immune detection.

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Specifications

MW Ranges Low 14,400–97,000 daltons

Protein MW (daltons) Range Range Range

Myosin 200,000 X X
β-galactosidase 116,250 X X
Phosphorylase b 97,400 X X X
Bovine serum albumin 66,200 X X X
Ovalbumin 45,000 X X X
Carbonic anhydrase 31,000 X X
Soybean trypsin inhibitor 21,500 X X
Lysozyme 14,400 X X
Aprotinin 6,500 X

Contents Approximately 130 µg total protein in a buffer

Volume 250 µl
Storage -20 °C
Shelf life 1 year at -20 °C
Applications Dilute 1:4 for HRP color development; 60–100

High 45,000–200,000 daltons
Broad 6,500–200,000 daltons

Low High Broad

of 50% glycerol, 150 mM NaCl, 3 mM NaN

applications per vial
Dilute 1:20 for AP color development; 300–500

applications per vial

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