Bio-Rad BioLogic Sample Loading Loops User Manual

0

5

10 15 20

25

01015 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90

5

DynaLoop

™

25

and

DynaLoop 90

Instruction

Manual

Catalog Numbers

750-0451 (25 ml)
750-0452 (90 ml)

For technical support, call your local Bio-Rad office, or in the U.S. call 1-800-4BIORAD (1-800-424-6723)

Table of Contents

Page

Section 1 Introduction.....................................................................................1

1.1 DynaLoop Sample Loop Description............................................................1

1.2 Operation ....................................................................................................1

Section 2 Installation.......................................................................................3

2.1 Unpacking ...................................................................................................3

2.2 Precautions and Recommendations ............................................................3

2.3 Installation ...................................................................................................4

Section 3 Using the DynaLoop.......................................................................9

3.1 Injecting Sample Volume .............................................................................9

Section 4 Care and Maintenance..................................................................11

4.1 Troubleshooting.........................................................................................11

4.2 Cleaning and Storage................................................................................12

4.3 Replacing the Filter....................................................................................13

4.4 Replacing O-Rings ....................................................................................14

Section 5 Appendix A Replacement Parts...................................................15

Section 6 Appendix B Solvent Compatibility...............................................15

List of Figures

Page

Figure 1 DynaLoop 25 and DynaLoop 90 Sample Loops ..........................1

Figure 2 Sliding Seal Assembly (Load Phase) ...........................................2

Figure 3 Sliding Seal Assembly (Flush Phase) ..........................................3

Figure 4 DynaLoop Plumbing Connections ...............................................5

Figure 5 Injection Valve Plumbing Connections........................................5

Figure 6 Injection Valve Positions ..............................................................6

Figure 7 Rear Panel of BioLogic Workstation with AUX Connector .........7

Figure 8 Peristaltic Pump Tubing Connections .........................................8

Figure 9 DynaLoop Cleaning ....................................................................12

Figure 10 Removing the Filter.....................................................................13

Tab l es

Table 1 Troubleshooting the DynaLoop..................................................11

Table 2 DynaLoop Replacement Parts....................................................15

Section 1
Introduction

1.1 DynaLoop Sample Loop Description

The DynaLoop sample loop is a large-volume sample injection loop for use with the

BioLogic DuoFlow

™

system. Available in 25 ml and 90 ml sample capacities, the DynaLoop

is used with the automated AVR7-3 injection valve.

1.2 Operation

The DynaLoop consists of five major components (Figure 1):

• Sample end assembly

• Buffer end assembly

• Glass tube graduated in 1 ml increments

• Sliding seal assembly

• Protective plastic jacket

Fig. 1. DynaLoop 25 and DynaLoop 90 sample loops.

The sliding seal assembly divides the glass tube into two distinct chambers:

• Sample chamber
This end connects to port 3 on the AVR7-3 injection valve.

• Buffer chamber
This end connects to port 6 on the AVR7-3 injection valve.

The DynaLoop sample loop delivers sample to the column in a four-phase process:

• Load phase

• Injection phase

• Flush phase

• Reload phase

1

Buffer end

Sliding seal
assembly

5

0 10 15 20 25 30 35 40 45 50 55 60 657075 80 85 90

Glass tube

10 1

5

0

5 20

assembly

25

Sample end
assembly

Plastic jacket

2

Load phase With the AVR7-3 injection valve in the LOAD position, sample is injected

into the sample chamber end of the DynaLoop via port 3 on the injection
valve using a peristaltic pump or a syringe. As the DynaLoop fills with sample,
the sliding seal moves toward the buffer end assembly. The sliding seal
O-ring and closed check valve within the sliding seal assembly prevent
mixing of buffer and sample (Figure 2).

Fig. 2. Sliding seal assembly. (Check valve is closed.)

Injection phase With the AVR7-3 injection valve in the INJECT position, buffer delivered from

the BioLogic gradient pump pushes against the sliding seal assembly, and
delivers sample to the column. No buffer passes through the seal assembly
into the sample chamber while the seal assembly is moving.

Flush phase When the sliding seal assembly contacts the sample end cap, the check

valve opens and buffer passes through the sliding seal. Sample is com­pletely flushed from the DynaLoop (Figure 3) onto the column in a concen­trated band. When the sample is loaded onto the column and sufficient
buffer has flushed all sample from the loop and check valve, the AVR7-3
injection valve will automatically switch to LOAD after the programmed
sample load volume is injected. The gradient pump is now
connected directly to the column, facilitating accurate gradient elutions.

O-ring seals

BUFFER

Check valve

(closed)

Sliding seal assembl

separates buffer from

sample

Sample end
connector

y

SAMPLE