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Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547
M1250131 Rev B
Standard
Cartridge Holder and
Deashing Holder
Instruction Manual
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Warranty
Holders received damaged in shipment or defective in workmanship will be
replaced if returned within 60 days of the
invoice date. No other warranty as to end
use, seal life, or performance is made.
Introduction
The Standard Cartridge Holder is a
convenient, reusable holder that will
accommodate any of Bio-Rad’s 30 x
4.6 mm HPLC cartridges. Its function is to
connect the cartridge to the HPLC system.
The Deashing Holder accommodates
two 30 x 4.6 mm cartridges in series. It is
typically used with the Cation H
+
and
CO
3
-
carbohydrate deashing cartridges.
Installation
The inlet and outlet connections on
the holders are zero dead volume connections which use compression type reverse
nuts and ferrules for attachment to the
HPLC system (see Figure 1). Nuts and
ferrules are provided with the holders. To
achieve the best results, new connections
should be swaged. This is mandatory
when replacing cartridge holders manufactured prior to March 1987. If tubing
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should be set at 0.1-0.2 ml/minute while
the cartridge is being inserted. To fit the
cartridge into the holder, remove one end
of the holder. Loosen the other end a few
turns. Insert the cartridge into the holder
with firm pressure. The cartridge will fit
into the holder securely. Fit the free end of
the holder onto the cartridge and screw it
down. With the Deashing Holder, the center fitting should be placed between the
two cartridges in the holder. The holder
ends need only be screwed down fingertight. Further tightening is unnecessary,
and may damage the seal or holder. Never
use tools to tighten this seal.
Temperature and
Pressure Limits
The maximum temperature for the
holder is 60 °C. System pressure should
not exceed 4,000 psi.
Leakage
If the cartridge holder leaks at less
than its rated pressure limit, do not try to
force a seal. A leak indicates that the seals
are either dirty, scratched, or damaged. If
the cartridge holder leaks, the seals should
be cleaned or replaced (Catalog number
125-0147).
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must be cut, file or grind the tubing end as
necessary so that the end cut is perpendicular to the tubing axis, and so that the
internal diameter has not been constricted.
Fig. 1. Connections to the holder.
Use 1/16" 316 stainless steel tubing,
0.010" ID, to connect the holder. See
Figure 1. Tighten the assembly of tubing,
ferrule, and nut finger-tight. Be sure the
tubing end bottoms squarely in the holder
fitting. Using a wrench, tighten the nut 3/4
turn past finger-tight. The ferrule will be
permanently set against the tubing. The
connection only needs to be tight enough
to seal, and should not be over-tightened.
Repeat this procedure for the other end of
the cartridge holder.
Cartridge Installation
Prior to inserting the cartridge, all air
should be removed from the tubing. After
all air bubbles are removed, the flow rate
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Nut Ferrule
Holder
1/16" stainless steel tubing
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When using a buffer as mobile phase,
rinse the holder’s end fittings with water
after removing the cartridge. This will
prevent salt precipitation which would
scratch or damage the seals.
Specifications
Materials in contact with the mobile
phase are 316 stainless steel and Kel-F
®
fluorocarbon.
Ordering Information
Catalog
Number Product Description
125-0131 Standard Cartridge Holder
125-0139 Deashing Holder
125-0028 1/16” Reverse Nuts with Ferrules, Parker
type (2 each)
125-0046 1/16” Reverse Nuts with Ferrules, Valco
type (2 each)
125-0027 HPLC Connector, 1/16” LDV Reverse Nut
Union (1)
125-0040 Stainless Steel Tubing, 1/16” x .010” ID x 3”
length, pkg. 5
125-0041 Stainless Steel Tubing, 1/16” x 0.10” ID x 6”
length, pkg. 5
125-0147 Seal Replacement Kit
125-0148 Replacement Seals
Kel-F is a registered trademark of the 3M Company.
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