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USER MANUAL
4501-1671 - C - en
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bioMérieux S.A. 69280 Marcy l'Etoile / France |
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29730 |
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RCS Lyon 673 620 399 |
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Version C |
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Tel. 33 (0)4 78 87 20 00 - Fax 33 (0)4 78 87 20 90 |
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http://www.biomerieux.com |
03/2009 |
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Revisions
The list of revisions below summarizes replacements or additional pages in your User Manual.
Version |
Date of printing |
Modifications |
Pages modified |
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A |
10/2008 |
Creation |
All |
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B |
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Not used |
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C |
03/2009 |
− New software version |
All |
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− Deletion of Modify button |
2-24 |
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− Addition of information for loading labels |
4-16 |
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− Modification to Figure 4-21 : Barcode (DOB removed) |
4-25 |
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− Correction of folder name in Instrument Log Files section |
7-6 |
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− New Maintenance Menu screenshot |
8-2 |
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− New order for performing maintenance operations |
8-3 to 8-20 |
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− Deletion of Label roll length sensing procedure |
8-28 |
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− Deletion of severity column in List of Error Messages |
9-1 to 9-9 |
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− Addition of Event no. 20110 in List of Error Messages |
9-6 |
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− Addition of Event no. 35026 in List of Error Messages |
9-9 |
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− Additions to Error, Cause and Recovery table |
9-11 to 9-14 |
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− Updated procedure for replacing the PREVITM Isola HEPA |
9-18 to 9-22 |
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filter |
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− New procedure for pipettor decontamination |
9-23 to 9-24 |
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− New procedure for reinitializing the instrument after the |
9-25 |
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pipettor descends into a plate on the process station |
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4501-1618 B en
W a r n i n g
The contents of this manual are based on the software release V 1.1.
This manual is periodically updated. The updates shall be included in the new releases of the Software.
Information supplied in this manual may be subject to modifications before the products described become available.
This manual may contain information or references relating to certain bioMérieux S.A. products, software or services which are not available in the country of release; this shall not mean that bioMérieux S.A. intends to market such products, software or services in such country
To request copies of publications or for any technical request, contact bioMérieux S.A. or your local distributor.
IMPORTANT! |
Use of the instrument and manual implies acceptance of the clauses below and the |
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clauses set out in the regulatory booklet. Users are invited to refer to these clauses. |
T r a d e m a r k s
bioMérieux, the blue logo, PREVI, VITEK and DENSICHEK are used, pending and/or registered trademarks belonging to bioMérieux S.A. or one of its subsidiaries.
Windows is a trademark belonging to Microsoft.
Monovette is a trademark of Sarstedt AG & Co.
Vacutainer is a trademark of Becton, Dickinson and Company.
ATCC is a trademark of American Type Culture Collection.
Sterilin is a trademark of Bibby Sterilin Ltd.
Microstreak is a trademark of Lab Tech.
Any other name or trademark is the property of its respective owner.
© 2008 - 2009 bioMérieux S.A.
Photos: bioMérieux / Printed in France / bioMérieux S.A. RCS Lyon 673 620 399
4501-1577 B en
|
SOFTWARE LICENSE AGREEMENT |
User: |
No.: |
Software: |
Release: |
Computer: |
Brand name: |
Printer: |
Brand name: |
Disk player: |
Brand name: |
The use of this Software is strictly governed by the following terms and conditions.
I - Purpose of this Agreement / Rights granted
bioMérieux S.A. hereby grants, to the User who, by using this manual, accepts a non-exclusive right to use the Software. The license is personal, non transferable, non assignable and does not comprise the right to grant sub-licenses.
The User expressly agrees, in its own name and behalf as well as in the name and on behalf of its employees, agents and collaborators, not to make any copy of the Software-except one single backup copy for archival purposesdisplay the Software on any computer other than the Computer, reproduce, sell, rent, lease, modify, adapt, translate or otherwise dispose of, all or part of the Software, the equipment, the user manual and its related documentation.
Specifically, the User agrees not to decompile, reverse engineer or disassemble all or part of the Software.
II - Intellectual Property
All intellectual property rights including patrimonial rights, in and to the Software are and shall remain bioMérieux S.A. exclusive property, subject to possible third party rights. Consequently, the User is not entitled to copy or reproduce the Software except as set forth above.
Except as expressly specified above, nothing contained herein shall be construed as conferring to the User any right, title or interest in and to the Software.
III - Warranties
Provided that this Agreement duly signed is received by bioMérieux S.A. within ten (10) business days from the delivery, bioMérieux S.A. warrants that the Software is free from defect in materials and workmanship under normal conditions of use during three (3) months from the delivery. Should any defect occur during such period, bioMérieux S.A. or one of its authorized distributors in the country of sale shall replace the Software provided that the User makes its request in writing together with copy of the Software invoice and, as the case may be, the defective media.
Apart from the foregoing, the Software is provided "AS IS" and no warranty, whether express or implied, of merchantability, safety, quality, or fitness for a particular purpose is given hereunder nor any warranty that the Software shall not infringe upon the intellectual property rights of any third party.
The warranty provided herein shall apply provided that Software is used under normal conditions and to the exclusion of any replacement caused by accidental or willful damage, or misuse of the software or accident whatsoever.
It is expressly understood that, according to this License, bioMérieux S.A. shall not perform any service related to the training, assistance or maintenance of the Software unless otherwise agreed upon between the parties through a separate written agreement setting forth the terms and conditions (in particular, financial conditions) of such training, assistance or maintenance.
IV - Liability
In no event shall bioMérieux S.A. be held liable for any consequences that may arise from any modifications made to the Software without its intervention, or, should the software be connected to the user’s network or system, for any malfunctions or damage to the software caused directly or indirectly by the network or system, in particular in the event of failure of the security system, or the consequences of installing software on the same hardware as this Software without the intervention and/or authorisation of bioMérieux S.A.
In no event shall bioMérieux S.A. be liable for any direct, indirect, special, consequential or nonconsequential, incidental, material or immaterial damage (including loss of goodwill, profits, data or any other economic advantage) related to, arising out of or in connection with, any use of the Software and/or its results by the User and/or any third party, including without limitation, its clients, customers and sub-contactors. User hereby agrees to indemnify, defend and hold bioMérieux S.A. harmless from all claims, damages, expenses, suits, losses or liabilities relating to, arising out of or in connection with User or such parties as well for any action of such third parties against bioMérieux S.A.
Should this provision be held unenforceable or void, the User irrevocably agrees that bioMérieux S.A. liability and expenses for all causes shall not exceed the total amount actually paid by the User hereunder for the software release concerned by the claim.
V - Applicable Law and settlement of disputes
This agreement is governed and construed in accordance with French Law.
Parties shall make their best efforts to settle any dispute through amicable discussions. In the event no amicable settlement is reached in the period of three (3) months after communication of the claim, any litigation shall be held in the exclusive jurisdiction of the courts of Lyon, France.
Done ..............................................................................
This…….....................day of..........................................
Signature and User's stamp:
COPY TO BE RETURNED TO bioMérieux S.A.
4501-1577 B en
|
SOFTWARE LICENSE AGREEMENT |
User: |
No.: |
Software: |
Release: |
Computer: |
Brand name: |
Printer: |
Brand name: |
Disk player: |
Brand name: |
The use of this Software is strictly governed by the following terms and conditions.
I - Purpose of this Agreement / Rights granted
bioMérieux S.A. hereby grants, to the User who, by using this manual, accepts a non-exclusive right to use the Software. The license is personal, non transferable, non assignable and does not comprise the right to grant sub-licenses.
The User expressly agrees, in its own name and behalf as well as in the name and on behalf of its employees, agents and collaborators, not to make any copy of the Software-except one single backup copy for archival purposesdisplay the Software on any computer other than the Computer, reproduce, sell, rent, lease, modify, adapt, translate or otherwise dispose of, all or part of the Software, the equipment, the user manual and its related documentation.
Specifically, the User agrees not to decompile, reverse engineer or disassemble all or part of the Software.
II - Intellectual Property
All intellectual property rights including patrimonial rights, in and to the Software are and shall remain bioMérieux S.A. exclusive property, subject to possible third party rights. Consequently, the User is not entitled to copy or reproduce the Software except as set forth above.
Except as expressly specified above, nothing contained herein shall be construed as conferring to the User any right, title or interest in and to the Software.
III - Warranties
Provided that this Agreement duly signed is received by bioMérieux S.A. within ten (10) business days from the delivery, bioMérieux S.A. warrants that the Software is free from defect in materials and workmanship under normal conditions of use during three (3) months from the delivery. Should any defect occur during such period, bioMérieux S.A. or one of its authorized distributors in the country of sale shall replace the Software provided that the User makes its request in writing together with copy of the Software invoice and, as the case may be, the defective media.
Apart from the foregoing, the Software is provided "AS IS" and no warranty, whether express or implied, of merchantability, safety, quality, or fitness for a particular purpose is given hereunder nor any warranty that the Software shall not infringe upon the intellectual property rights of any third party.
The warranty provided herein shall apply provided that Software is used under normal conditions and to the exclusion of any replacement caused by accidental or willful damage, or misuse of the software or accident whatsoever.
It is expressly understood that, according to this License, bioMérieux S.A. shall not perform any service related to the training, assistance or maintenance of the Software unless otherwise agreed upon between the parties through a separate written agreement setting forth the terms and conditions (in particular, financial conditions) of such training, assistance or maintenance.
IV - Liability
In no event shall bioMérieux S.A. be held liable for any consequences that may arise from any modifications made to the Software without its intervention, or, should the software be connected to the user’s network or system, for any malfunctions or damage to the software caused directly or indirectly by the network or system, in particular in the event of failure of the security system, or the consequences of installing software on the same hardware as this Software without the intervention and/or authorisation of bioMérieux S.A.
In no event shall bioMérieux S.A. be liable for any direct, indirect, special, consequential or nonconsequential, incidental, material or immaterial damage (including loss of goodwill, profits, data or any other economic advantage) related to, arising out of or in connection with, any use of the Software and/or its results by the User and/or any third party, including without limitation, its clients, customers and sub-contactors. User hereby agrees to indemnify, defend and hold bioMérieux S.A. harmless from all claims, damages, expenses, suits, losses or liabilities relating to, arising out of or in connection with User or such parties as well for any action of such third parties against bioMérieux S.A.
Should this provision be held unenforceable or void, the User irrevocably agrees that bioMérieux S.A. liability and expenses for all causes shall not exceed the total amount actually paid by the User hereunder for the software release concerned by the claim.
V - Applicable Law and settlement of disputes
This agreement is governed and construed in accordance with French Law.
Parties shall make their best efforts to settle any dispute through amicable discussions. In the event no amicable settlement is reached in the period of three (3) months after communication of the claim, any litigation shall be held in the exclusive jurisdiction of the courts of Lyon, France.
Done ..............................................................................
This…….....................day of..........................................
Signature and User's stamp:
COPY TO BE KEPT BY USER
Table of Contents
1 How to use this Manual
Finding topics and procedures .................................................................................................................................. |
1-1 |
Warnings................................................................................................................................................................... |
1-1 |
2 Description
Intended Use............................................................................................................................................................. |
2-1 |
System Capabilities................................................................................................................................................... |
2-1 |
Workflow Principle..................................................................................................................................................... |
2-2 |
System Architecture.................................................................................................................................................. |
2-3 |
System Components................................................................................................................................................. |
2-4 |
Workstation ............................................................................................................................................................... |
2-5 |
Description......................................................................................................................................................... |
2-5 |
Instrument ................................................................................................................................................................. |
2-6 |
Sample racks ..................................................................................................................................................... |
2-9 |
Sample tubes................................................................................................................................................... |
2-10 |
Tubes compatible for use with the PREVI Isola ....................................................................................... |
2-10 |
Fill heights................................................................................................................................................. |
2-10 |
Prepoured media (Plates) ................................................................................................................................ |
2-10 |
Plates compatible for use with the PREVI Isola ....................................................................................... |
2-10 |
Input cassettes................................................................................................................................................. |
2-11 |
Output cassettes .............................................................................................................................................. |
2-12 |
Touch-screen................................................................................................................................................... |
2-12 |
PREVI Isola Waste Paper Bin ......................................................................................................................... |
2-13 |
Waste drawer................................................................................................................................................... |
2-13 |
Interlocks................................................................................................................................................................. |
2-14 |
Consumables .......................................................................................................................................................... |
2-15 |
PREVI Isola Applicators .................................................................................................................................. |
2-15 |
PREVI Isola Applicator cartridge ..................................................................................................................... |
2-15 |
PREVI Isola Tips ............................................................................................................................................. |
2-16 |
PREVI Isola Labels ......................................................................................................................................... |
2-16 |
PREVI Isola biohazardous Waste Bin ............................................................................................................. |
2-16 |
PREVI Isola HEPA filter .................................................................................................................................. |
2-17 |
Instrument Operating Principle................................................................................................................................ |
2-18 |
Index next sample............................................................................................................................................ |
2-19 |
Read sample barcode...................................................................................................................................... |
2-19 |
Retrieve sample data ....................................................................................................................................... |
2-20 |
Pick up tip ........................................................................................................................................................ |
2-20 |
Aspirate sample ............................................................................................................................................... |
2-20 |
Perform plate workflow .................................................................................................................................... |
2-21 |
Retrieve input plate................................................................................................................................... |
2-21 |
Transfer to plate transfer robot ................................................................................................................. |
2-21 |
Transfer to process station ....................................................................................................................... |
2-21 |
Measure agar height, dispense sample .................................................................................................... |
2-21 |
Pick up applicator ..................................................................................................................................... |
2-21 |
Streak plate............................................................................................................................................... |
2-21 |
Discard applicator ..................................................................................................................................... |
2-21 |
If Bi – plate................................................................................................................................................ |
2-21 |
Transfer to transfer robot .......................................................................................................................... |
2-21 |
Label plate ................................................................................................................................................ |
2-22 |
Transfer to output robot ............................................................................................................................ |
2-22 |
Dispose of tip ................................................................................................................................................... |
2-22 |
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PREVITM Isola User Manual |
I-1 |
Table of Contents
Workstation Software .............................................................................................................................................. |
2-23 |
Principle ........................................................................................................................................................... |
2-23 |
General screen layout...................................................................................................................................... |
2-23 |
Menu bar.......................................................................................................................................................... |
2-24 |
Title and button bar .......................................................................................................................................... |
2-24 |
Working area ................................................................................................................................................... |
2-24 |
Status bar ........................................................................................................................................................ |
2-24 |
Instrument Software................................................................................................................................................ |
2-25 |
Main screen and general layout....................................................................................................................... |
2-25 |
Alarm zone....................................................................................................................................................... |
2-26 |
Title bar............................................................................................................................................................ |
2-26 |
Status bar ........................................................................................................................................................ |
2-26 |
Navigation/action bar ....................................................................................................................................... |
2-27 |
Working area ................................................................................................................................................... |
2-28 |
3 Getting Started
Assembly and Installation ......................................................................................................................................... |
3-1 |
Choosing a location ........................................................................................................................................... |
3-1 |
Electrical connections ........................................................................................................................................ |
3-1 |
Hazardous Magnetic Fields Precautions................................................................................................................... |
3-1 |
Moving the Instrument............................................................................................................................................... |
3-1 |
Barcode Reader Installation...................................................................................................................................... |
3-1 |
Installation of the PREVI Isola and BCI .................................................................................................................... |
3-2 |
Checking installation .......................................................................................................................................... |
3-2 |
Powering up the Workstation .................................................................................................................................... |
3-3 |
Connecting to BCI NET ..................................................................................................................................... |
3-3 |
Connecting to BCI RS232.................................................................................................................................. |
3-3 |
Starting the Workstation Software............................................................................................................................. |
3-4 |
Entry of an incorrect user name or password .................................................................................................... |
3-4 |
Changing work sessions .................................................................................................................................... |
3-5 |
Inactivity timeout ................................................................................................................................................ |
3-5 |
Defining Plate Panels................................................................................................................................................ |
3-6 |
Creating a plate panel........................................................................................................................................ |
3-7 |
Modifying a plate panel ...................................................................................................................................... |
3-9 |
Deleting a plate panel ........................................................................................................................................ |
3-9 |
Printing a plate panel ....................................................................................................................................... |
3-10 |
Defining Plate Types ............................................................................................................................................... |
3-11 |
Creating a plate type........................................................................................................................................ |
3-11 |
Modifying a plate type ...................................................................................................................................... |
3-12 |
Deleting a plate type ........................................................................................................................................ |
3-12 |
Defining Incubation Types....................................................................................................................................... |
3-13 |
Creating an incubation type ............................................................................................................................. |
3-13 |
Modifying an incubation type ........................................................................................................................... |
3-14 |
Deleting an incubation type.............................................................................................................................. |
3-14 |
Assigning Plate Panels to Specimen Types............................................................................................................ |
3-15 |
Printing plate panels assigned to specimen types ........................................................................................... |
3-17 |
Automatically Assigning Plate Panels to Samples .................................................................................................. |
3-18 |
Defining Plate Cassettes Configurations................................................................................................................. |
3-19 |
Creating a plate cassettes configuration .......................................................................................................... |
3-19 |
Modifying a plate cassettes configuration ........................................................................................................ |
3-21 |
Deleting a plate cassettes configuration .......................................................................................................... |
3-21 |
Assigning Plate Panels to Samples ........................................................................................................................ |
3-22 |
Viewing and Printing Worklists................................................................................................................................ |
3-24 |
General Settings ..................................................................................................................................................... |
3-26 |
I-2 |
PREVITM Isola User Manual |
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Table of Contents |
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4 Using the System |
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Basic Workflow ......................................................................................................................................................... |
4-1 |
Starting the System................................................................................................................................................... |
4-2 |
Powering up the instrument ............................................................................................................................... |
4-2 |
Screensaver....................................................................................................................................................... |
4-2 |
Prerequisites for Sample Processing ........................................................................................................................ |
4-4 |
Pre-start Checks ....................................................................................................................................................... |
4-4 |
Selecting Plates ........................................................................................................................................................ |
4-5 |
Loading Plates .......................................................................................................................................................... |
4-7 |
Loading plates during processing ...................................................................................................................... |
4-7 |
Loading plates without removing the input cassette........................................................................................... |
4-8 |
Plate cassettes status ........................................................................................................................................ |
4-9 |
Loading PREVI Isola Applicators............................................................................................................................ |
4-10 |
Loading PREVI Isola Tips....................................................................................................................................... |
4-13 |
Loading PREVI Isola Labels................................................................................................................................... |
4-15 |
Monitoring Consumables and Waste Bins Status ................................................................................................... |
4-17 |
Consumables................................................................................................................................................... |
4-17 |
PREVI Isola Waste Bins.................................................................................................................................. |
4-18 |
Sample Preparation ................................................................................................................................................ |
4-19 |
Recommended procedures.............................................................................................................................. |
4-19 |
Urine ......................................................................................................................................................... |
4-19 |
Feces........................................................................................................................................................ |
4-19 |
Dry mono swab......................................................................................................................................... |
4-19 |
Swab in a liquid transport medium............................................................................................................ |
4-19 |
Swab in gel transport medium .................................................................................................................. |
4-19 |
Blood ........................................................................................................................................................ |
4-19 |
Liquid respiratory samples........................................................................................................................ |
4-19 |
Placing Sample Tubes in Racks ............................................................................................................................. |
4-20 |
Loading Sample Racks ........................................................................................................................................... |
4-21 |
Processing Samples ............................................................................................................................................... |
4-23 |
Processing hints............................................................................................................................................... |
4-27 |
Pausing the Instrument ........................................................................................................................................... |
4-28 |
Instrument-generated pause............................................................................................................................ |
4-28 |
User-generated pause ..................................................................................................................................... |
4-28 |
Stopping the Instrument .......................................................................................................................................... |
4-29 |
Instrument-generated stop (Error case) ........................................................................................................... |
4-29 |
Resuming operations after stopping ......................................................................................................... |
4-29 |
User-generated stop ........................................................................................................................................ |
4-31 |
Resuming operations after stopping ......................................................................................................... |
4-31 |
Unloading Processed Plates ................................................................................................................................... |
4-32 |
Unloading processed plates on completion of processing ............................................................................... |
4-32 |
Unloading processed plates during processing ............................................................................................... |
4-33 |
Remove a full output cassette................................................................................................................... |
4-33 |
Remove plates from the cassettes in situ ................................................................................................. |
4-33 |
Unloading unprocessed plates......................................................................................................................... |
4-33 |
Unloading Sample Tubes........................................................................................................................................ |
4-34 |
Removing Waste..................................................................................................................................................... |
4-35 |
PREVI Isola biohazardous Waste Bin ............................................................................................................. |
4-35 |
PREVI Isola Waste Paper Bin ......................................................................................................................... |
4-36 |
Powering Down the Instrument ............................................................................................................................... |
4-37 |
Examples of Correct Streaking Patterns ................................................................................................................. |
4-38 |
PREVITM Isola User Manual |
I-3 |
Table of Contents
5 Quality Control |
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Intended Use............................................................................................................................................................. |
5-1 |
Material ..................................................................................................................................................................... |
5-1 |
Workstation Preparation............................................................................................................................................ |
5-1 |
Instrument Preparation.............................................................................................................................................. |
5-2 |
Sample Tube Preparation ......................................................................................................................................... |
5-2 |
Sample Run and Plate Incubation............................................................................................................................. |
5-2 |
Results ...................................................................................................................................................................... |
5-2 |
Score determination.................................................................................................................................................. |
5-3 |
Reference Appearance ............................................................................................................................................. |
5-3 |
Results ...................................................................................................................................................................... |
5-4 |
6 Information Technology Security |
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User Management..................................................................................................................................................... |
6-1 |
User groups ....................................................................................................................................................... |
6-1 |
Creating a new user account ............................................................................................................................. |
6-2 |
Assigning user permissions and rights............................................................................................................... |
6-5 |
Disabling a user account.................................................................................................................................. |
6-10 |
Unlocking a user account................................................................................................................................. |
6-11 |
Changing of a password by a user................................................................................................................... |
6-12 |
Antivirus .................................................................................................................................................................. |
6-13 |
Windows Update..................................................................................................................................................... |
6-13 |
IT Verification Protocol ............................................................................................................................................ |
6-13 |
Printer Verification Protocol..................................................................................................................................... |
6-14 |
7 Data Backup, Restore and Audit Trail |
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Presentation.............................................................................................................................................................. |
7-1 |
Manual data backup.................................................................................................................................................. |
7-1 |
Data restoration from a removable medium .............................................................................................................. |
7-1 |
Audit trail data viewing using the event viewer.......................................................................................................... |
7-2 |
Saving the Audit Trail................................................................................................................................................ |
7-4 |
Instrument and Workstation Log File Retrieval ......................................................................................................... |
7-6 |
Instrument log files............................................................................................................................................. |
7-6 |
Workstation and data server log files ................................................................................................................. |
7-6 |
Full System Backup and Restoration ........................................................................................................................ |
7-7 |
Full System Backup ........................................................................................................................................... |
7-7 |
Full System Restoration..................................................................................................................................... |
7-7 |
8 Maintenance
Introduction ............................................................................................................................................................... |
8-1 |
Safety Precautions and Controls............................................................................................................................... |
8-1 |
Biological safety requirements ........................................................................................................................... |
8-1 |
Recommended maintenance product ................................................................................................................ |
8-1 |
Presentation of the Maintenance Menu..................................................................................................................... |
8-2 |
Maintenance Logs..................................................................................................................................................... |
8-3 |
Daily Maintenance Procedures ................................................................................................................................. |
8-4 |
Clean the tray under input cassettes.................................................................................................................. |
8-4 |
Clean the waste chute top, the area around the waste chute top and the waste chute chimney ....................... |
8-5 |
Clean the process station drip tray..................................................................................................................... |
8-6 |
Clean the pipettor, drip tray and area under and around the drip tray................................................................ |
8-7 |
Clean the sample racks ..................................................................................................................................... |
8-8 |
Clean the sample rack tray and sample spill tray............................................................................................... |
8-9 |
Clean the tray under output cassettes ............................................................................................................. |
8-11 |
Clean the waste chute deflector....................................................................................................................... |
8-12 |
Check, replace or empty the waste bins, and clean the deflector .................................................................... |
8-13 |
I-4 |
PREVITM Isola User Manual |
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Table of Contents |
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Weekly Maintenance Procedures ........................................................................................................................... |
8-14 |
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Clean the inside of input and output cassettes ................................................................................................ |
8-14 |
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Clean the area around the applicator cartridge and the tip tray ....................................................................... |
8-15 |
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Clean the process station, reference block, transfer robot suction cups, and agar sensor .............................. |
8-15 |
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Clean the printer and the printer platen............................................................................................................ |
8-18 |
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Clean the print head......................................................................................................................................... |
8-19 |
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Clean the touch-screen.................................................................................................................................... |
8-19 |
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Clean the external surfaces of the instrument.................................................................................................. |
8-20 |
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Clean the waste drawer ................................................................................................................................... |
8-20 |
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Maintenance Operations Performed by bioMérieux ................................................................................................ |
8-21 |
|
Spill Management ................................................................................................................................................... |
8-21 |
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Materials required ............................................................................................................................................ |
8-21 |
|
Decontamination Procedures.................................................................................................................................. |
8-21 |
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Basic decontamination..................................................................................................................................... |
8-21 |
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Removal and Disposal of Materials and Waste....................................................................................................... |
8-22 |
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Disposal of used consumables ........................................................................................................................ |
8-22 |
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Loading PREVI Isola Label Rolls............................................................................................................................ |
8-23 |
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Replacing Fuses ..................................................................................................................................................... |
8-29 |
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Replacing the PREVI Isola HEPA Filter.................................................................................................................. |
8-29 |
9 |
Troubleshooting |
|
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Introduction ............................................................................................................................................................... |
9-1 |
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List of Error Messages .............................................................................................................................................. |
9-2 |
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Event Log................................................................................................................................................................ |
9-10 |
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Error, Cause and Recovery..................................................................................................................................... |
9-11 |
|
Recovery from events with “Warning” severity........................................................................................................ |
9-15 |
|
Recovery from events with “Error” severity ............................................................................................................. |
9-15 |
|
Recovery from Events with “Fatal” Severity ............................................................................................................ |
9-16 |
|
Restarting the Instrument Software......................................................................................................................... |
9-16 |
|
PREVI Isola HEPA Filter ........................................................................................................................................ |
9-17 |
|
Replacing the PREVI Isola HEPA filter............................................................................................................ |
9-18 |
|
Pipettor.................................................................................................................................................................... |
9-23 |
|
Clean and decontaminate the pipettor if it descends into a sample tube ......................................................... |
9-23 |
|
Material required....................................................................................................................................... |
9-23 |
|
Procedure ................................................................................................................................................. |
9-23 |
|
Possible issues after cleaning................................................................................................................... |
9-25 |
|
Reinitialize the instrument after the pipettor descends into a plate on the process station .............................. |
9-25 |
|
Procedure ................................................................................................................................................. |
9-25 |
|
Examples of Incorrect Inoculation and Streaking Patterns...................................................................................... |
9-26 |
|
Back up Instrument Log Files.................................................................................................................................. |
9-27 |
|
Anonymization of Patient Data................................................................................................................................ |
9-27 |
10 |
General System Characteristics |
|
|
Dimensions ...................................................................................................................................................... |
10-1 |
|
Weight.............................................................................................................................................................. |
10-1 |
|
Electrical specifications.................................................................................................................................... |
10-1 |
|
Environmental considerations.......................................................................................................................... |
10-1 |
PREVITM Isola User Manual |
I-5 |
Table of Contents
11 |
Appendices |
|
|
Appendix A: Packing list.......................................................................................................................................... |
11-1 |
|
Instrument kit ................................................................................................................................................... |
11-1 |
|
User Manual kit................................................................................................................................................ |
11-1 |
|
Workstation PC kit ........................................................................................................................................... |
11-1 |
|
Appendix B: Stopping and Restarting the Data Server ........................................................................................... |
11-2 |
|
Stopping the data server.................................................................................................................................. |
11-2 |
|
Restarting the data server................................................................................................................................ |
11-4 |
12 |
Glossary |
|
I-6 |
PREVITM Isola User Manual |
List of Figures
2 Description |
|
Figure 2-1 : Workflow Principle ................................................................................................................................. |
2-2 |
Figure 2-2 : System Architecture............................................................................................................................... |
2-3 |
Figure 2-3 : System Components ............................................................................................................................. |
2-4 |
Figure 2-4 : Front view of the PREVI Isola with covers on........................................................................................ |
2-6 |
Figure 2-5 : Front view of the PREVI Isola – front covers removed.......................................................................... |
2-7 |
Figure 2-6 : Back view of the PREVI Isola with covers on ........................................................................................ |
2-8 |
Figure 2-7 : Sample racks ......................................................................................................................................... |
2-9 |
Figure 2-8 : Fill heights............................................................................................................................................ |
2-10 |
Figure 2-9 : Input cassette – front ........................................................................................................................... |
2-11 |
Figure 2-10 : Input cassette – back......................................................................................................................... |
2-11 |
Figure 2-11 : Output cassette - front ....................................................................................................................... |
2-12 |
Figure 2-12 : Output cassette - back....................................................................................................................... |
2-12 |
Figure 2-13: Touch-screen...................................................................................................................................... |
2-12 |
Figure 2-14 : PREVI Isola Waste Paper Bin ........................................................................................................... |
2-13 |
Figure 2-15 : Waste Drawer .................................................................................................................................... |
2-13 |
Figure 2-16 : PREVI Isola Applicator...................................................................................................................... |
2-15 |
Figure 2-17 : PREVI Isola Applicator cartridge ....................................................................................................... |
2-15 |
Figure 2-18 : PREVI Isola Tips in tray .................................................................................................................... |
2-16 |
Figure 2-19 : Simplified sample workflow................................................................................................................ |
2-18 |
Figure 2-20 : Positioning a barcode label on a tube................................................................................................ |
2-19 |
Figure 2-21 : General screen layout........................................................................................................................ |
2-23 |
Figure 2-22 : Main screen layout............................................................................................................................. |
2-25 |
Figure 2-23 : Working area ..................................................................................................................................... |
2-28 |
3 Getting Started
Figure 3-1 : Login pop-up window............................................................................................................................. |
3-4 |
Figure 3-2 : “Invalid user name / password” window................................................................................................. |
3-4 |
Figure 3-3 : Workstation software shutdown window ................................................................................................ |
3-5 |
Figure 3-4 : “Plate Panels” screen ............................................................................................................................ |
3-6 |
Figure 3-5 : Create a plate panel .............................................................................................................................. |
3-7 |
Figure 3-6 : “Plate Panel” error message .................................................................................................................. |
3-8 |
Figure 3-7 : “Invalid entry” error message ................................................................................................................. |
3-8 |
Figure 3-8 : “Delete a plate panel” confirmation message......................................................................................... |
3-9 |
Figure 3-9 : “Plate Panel Printing” selection window............................................................................................... |
3-10 |
Figure 3-10 : Plate panel “Print Preview” window ................................................................................................... |
3-10 |
Figure 3-11 : “Plate Panels” plate types screen ...................................................................................................... |
3-11 |
Figure 3-12 : Define incubation types...................................................................................................................... |
3-13 |
Figure 3-13 : Incubation types................................................................................................................................. |
3-14 |
Figure 3-14 : “Specimen Types” screen .................................................................................................................. |
3-15 |
Figure 3-15 : Specimen types “Print Preview” window............................................................................................ |
3-17 |
Figure 3-16 : “Plate Cassettes Configuration” screen ............................................................................................. |
3-19 |
Figure 3-17 : New plate cassettes configuration ..................................................................................................... |
3-20 |
Figure 3-18 : “Invalid Input” error message ............................................................................................................. |
3-20 |
Figure 3-19 : “Delete a plate cassettes configuration” confirmation message......................................................... |
3-21 |
Figure 3-20 : “Sample Assignment“ screen............................................................................................................. |
3-22 |
Figure 3-21 : “Sample Assignment“ screen with sample ......................................................................................... |
3-23 |
Figure 3-22 : “Worklist” screen................................................................................................................................ |
3-24 |
Figure 3-23 : Worklist “Print Preview” window......................................................................................................... |
3-25 |
Figure 3-24 : “General Settings” screen .................................................................................................................. |
3-26 |
|
|
PREVITM Isola User Manual |
II-1 |
List of Figures
4 Using the System |
|
Figure 4-1 : Basic workflow diagram ......................................................................................................................... |
4-1 |
Figure 4-2 : Powering up the instrument ................................................................................................................... |
4-2 |
Figure 4-3 : Main screen ........................................................................................................................................... |
4-3 |
Figure 4-4 : “Select Plates” screen............................................................................................................................ |
4-5 |
Figure 4-5 : Load plates agar-side up ....................................................................................................................... |
4-7 |
Figure 4-6 : Loading plates without removing the input cassette .............................................................................. |
4-8 |
Figure 4-7 : Main screen – “Cassettes status” .......................................................................................................... |
4-9 |
Figure 4-8 : “Load Applicators and Tips” screen ..................................................................................................... |
4-10 |
Figure 4-9 : Pulling the top lock............................................................................................................................... |
4-11 |
Figure 4-10 : Inserting the cartridge ........................................................................................................................ |
4-11 |
Figure 4-11 : “Load Applicators and Tips” screen ................................................................................................... |
4-13 |
Figure 4-12 : Inserting the PREVI Isola Tip tray ..................................................................................................... |
4-14 |
Figure 4-13 : “Load Labels” screen ......................................................................................................................... |
4-15 |
Figure 4-14 : "Main screen – consumable status"................................................................................................... |
4-17 |
Figure 4-15 : Sample tubes in racks........................................................................................................................ |
4-20 |
Figure 4-16 : Correct position of tubes in rack ........................................................................................................ |
4-20 |
Figure 4-17 : “Load Samples” screen...................................................................................................................... |
4-21 |
Figure 4-18 : Load sample racks............................................................................................................................. |
4-22 |
Figure 4-19 : “Preparing to start processing …” screen .......................................................................................... |
4-23 |
Figure 4-20 : Processing screen ............................................................................................................................. |
4-24 |
Figure 4-21 : Barcode ............................................................................................................................................. |
4-25 |
Figure 4-22 : Sample processing status.................................................................................................................. |
4-26 |
Figure 4-23 : “Processing Complete” screen........................................................................................................... |
4-27 |
Figure 4-24 : “Fatal Error” screen............................................................................................................................ |
4-29 |
Figure 4-25 : “Shutdown Requested” window – instrument processing .................................................................. |
4-30 |
Figure 4-26 : Opening guard and removing output cassette ................................................................................... |
4-32 |
Figure 4-27 : Correct streaking patterns.................................................................................................................. |
4-38 |
5 |
Quality Control |
|
|
Figure 5-1 : Score determination.............................................................................................................................. |
5-3 |
|
Figure 5-2 : Reference appearance ......................................................................................................................... |
5-3 |
6 |
Information Technology Security |
|
|
Figure 6-1 : Opening the Computer Management window........................................................................................ |
6-2 |
|
Figure 6-2 : "Computer Management" screen........................................................................................................... |
6-2 |
|
Figure 6-3 : "New User" screen................................................................................................................................. |
6-3 |
|
Figure 6-4 : "New User" creation box ........................................................................................................................ |
6-3 |
|
Figure 6-5 : "New User" creation box with data entered............................................................................................ |
6-4 |
|
Figure 6-6 : "User Rights Management" screen........................................................................................................ |
6-5 |
|
Figure 6-7 : User “Properties" screen........................................................................................................................ |
6-5 |
|
Figure 6-8 : "User properties" – " Member Of “ tab screen........................................................................................ |
6-6 |
|
Figure 6-9 : "Select Groups" screen.......................................................................................................................... |
6-6 |
|
Figure 6-10 : "Select Groups" screen (cont’d)........................................................................................................... |
6-7 |
|
Figure 6-11 : "Select Groups" screen (cont’d)........................................................................................................... |
6-7 |
|
Figure 6-12 : "Select Groups" screen (cont’d)........................................................................................................... |
6-8 |
|
Figure 6-13 : "Select Groups" screen (cont’d)........................................................................................................... |
6-8 |
|
Figure 6-14 : "Properties" screen – "Member Of" tab screen .................................................................................... |
6-9 |
|
Figure 6-15 : "User Rights Management" screen.................................................................................................... |
6-10 |
|
Figure 6-16 : "User Properties" - "General" tab screen ........................................................................................... |
6-10 |
|
Figure 6-17 : "User Rights Management" screen.................................................................................................... |
6-11 |
|
Figure 6-18 : " Windows Security" window................................................................................................................ |
6-12 |
|
Figure 6-19 : "Change Password" window .............................................................................................................. |
6-12 |
II-2 |
PREVITM Isola User Manual |
|
|
List of Figures |
|
|
|
7 Data Backup, Restore and Audit Trail |
|
|
|
Figure 7-1 : Event viewer shortcut ............................................................................................................................ |
7-2 |
|
Figure 7-2 : “Event viewer” window........................................................................................................................... |
7-2 |
|
Figure 7-3 : Event viewer “AS_Audit” window ........................................................................................................... |
7-3 |
|
Figure 7-4 : “Event Properties” window..................................................................................................................... |
7-3 |
|
Figure 7-5 : “Save Log File AS…” window ................................................................................................................ |
7-4 |
|
Figure 7-6 : Save “AS_Audit” As… window............................................................................................................... |
7-5 |
8 |
Maintenance |
|
|
Figure 8-1 : Maintenance Menu.................................................................................................................................. |
8-2 |
|
Figure 8-2 : Removing the tray under the input cassettes ............................................................................................ |
8-4 |
|
Figure 8-3 : Removing the waste chute top................................................................................................................. |
8-5 |
|
Figure 8-4 : Removing the process station drip tray..................................................................................................... |
8-6 |
|
Figure 8-5 : Cleaning the pipettor ............................................................................................................................... |
8-7 |
|
Figure 8-6 : Drip tray to the right of the process station................................................................................................ |
8-8 |
|
Figure 8-7 : Sample rack tray presented for cleaning................................................................................................... |
8-9 |
|
Figure 8-8 : Applying cleaning solution ....................................................................................................................... |
8-9 |
|
Figure 8-9 : Removing the sample spill tray .............................................................................................................. |
8-10 |
|
Figure 8-10 : Lifting up the sample spill tray .............................................................................................................. |
8-10 |
|
Figure 8-11 : Removing the tray under output cassettes............................................................................................ |
8-11 |
|
Figure 8-12 : Removing the waste chute deflector ................................................................................................... |
8-12 |
|
Figure 8-13 : Waste chute deflector ........................................................................................................................ |
8-12 |
|
Figure 8-14 : PREVI Isola Waste Bins in drawer....................................................................................................... |
8-13 |
|
Figure 8-15 : Cleaning inside cassettes .................................................................................................................... |
8-14 |
|
Figure 8-16 : Wiping inside cassettes ..................................................................................................................... |
8-14 |
|
Figure 8-17 : Cleaning the process station.............................................................................................................. |
8-15 |
|
Figure 8-18 : Reference block................................................................................................................................. |
8-16 |
|
Figure 8-19 : Robot suction cups ............................................................................................................................ |
8-16 |
|
Figure 8-20 : Cleaning the agar sensor................................................................................................................... |
8-17 |
|
Figure 8-21 : Cleaning the platen............................................................................................................................ |
8-18 |
|
Figure 8-22 : Print head .......................................................................................................................................... |
8-19 |
|
Figure 8-23 : Raising the roller arms....................................................................................................................... |
8-23 |
|
Figure 8-24 : Raising the release latches................................................................................................................ |
8-23 |
|
Figure 8-25 : Removing the empty roll .................................................................................................................... |
8-23 |
|
Figure 8-26 : Correct position of new roll ................................................................................................................ |
8-24 |
|
Figure 8-27 : Using the guides ................................................................................................................................ |
8-24 |
|
Figure 8-28 : Feeding label backing through the printer.......................................................................................... |
8-25 |
|
Figure 8-29 : Instructions for loading labels on instrument touch-screen ................................................................ |
8-25 |
|
Figure 8-30 : Pull mechanism slot (top view) .......................................................................................................... |
8-26 |
|
Figure 8-31 : Label backing correctly pulled into the slot (top view)........................................................................ |
8-26 |
|
Figure 8-32 : Lowering the roller arms .................................................................................................................... |
8-26 |
|
Figure 8-33 : Turning the label roll .......................................................................................................................... |
8-27 |
|
Figure 8-34 : Metal plate not to be removed ........................................................................................................... |
8-27 |
|
Figure 8-35 : Label backing coming out of deflector ............................................................................................... |
8-28 |
|
Figure 8-36 : Main power inlet fuses ....................................................................................................................... |
8-29 |
9 |
Troubleshooting |
|
|
Figure 9-1 : Event Log screen................................................................................................................................. |
9-10 |
|
Figure 9-2 : Warning Message................................................................................................................................ |
9-15 |
|
Figure 9-3 : Error Message ..................................................................................................................................... |
9-15 |
|
Figure 9-4 : Fatal Message ..................................................................................................................................... |
9-16 |
|
Figure 9-5 : PREVI Isola HEPA filter error message .............................................................................................. |
9-17 |
|
Figure 9-6 : Unlocking the latch .............................................................................................................................. |
9-18 |
|
Figure 9-7 : Unhooking the latch ............................................................................................................................. |
9-18 |
|
|
|
PREVITM Isola User Manual |
II-3 |
|
List of Figures
Figure 9-8 : Preparing to place the PREVI Isola HEPA filter in a plastic bag.......................................................... |
9-19 |
|
Figure 9-9 : Sliding the PREVI Isola HEPA filter out of its housing......................................................................... |
9-19 |
|
Figure 9-10 : Removing the PREVI Isola HEPA filter from its housing ................................................................... |
9-20 |
|
Figure 9-11 : Covering the PREVI Isola HEPA filter as it comes out of its housing ................................................ |
9-20 |
|
Figure 9-12 : Covering the PREVI Isola HEPA filter until fully extracted................................................................. |
9-20 |
|
Figure 9-13 : PREVI Isola HEPA filter in the plastic bag......................................................................................... |
9-21 |
|
Figure 9-14 : New PREVI Isola HEPA filter in place............................................................................................... |
9-21 |
|
Figure 9-15 : Unscrew the pipettor barrel................................................................................................................ |
9-23 |
|
Figure 9-16 : Cleaning the black pipettor piston...................................................................................................... |
9-24 |
|
Figure 9-17 : Cleaning the inside of the pipettor barrel ........................................................................................... |
9-24 |
|
Figure 9-18 : Cleaning the outisde of the pipettor barrel ......................................................................................... |
9-24 |
|
Figure 9-19 |
: Pipettor that has descended into a plate............................................................................................ |
9-25 |
Figure 9-20 |
: Incorrect inoculation and streaking patterns ...................................................................................... |
9-26 |
Figure 9-21 |
: “Database Anonymiser” screen ......................................................................................................... |
9-27 |
Figure 9-22 |
: Database successfully anonymized................................................................................................... |
9-27 |
11 Appendices
Figure 11-1 : Opening the “Services” window ......................................................................................................... |
11-2 |
|
Figure 11-2 |
: “Services” window.............................................................................................................................. |
11-2 |
Figure 11-3 |
: “DataServer” selection window .......................................................................................................... |
11-3 |
Figure 11-4 |
: “Service Control” dialog box............................................................................................................... |
11-3 |
Figure 11-5 |
: Selecting the “Start” link..................................................................................................................... |
11-4 |
Screenshots and figures are given for information purposes only.
II-4 |
PREVITM Isola User Manual |
List of Tables
2. Description
Table |
2-1 |
: Workstation software buttons............................................................................................................... |
2-24 |
Table |
2-2 |
: Alarm types .......................................................................................................................................... |
2-26 |
Table |
2-3 |
: Navigation/action bar buttons............................................................................................................... |
2-27 |
4. Using the System
Table |
4-1 |
: Plate cassettes status icons................................................................................................................... |
4-9 |
Table |
4-2 |
: Consumables status icons ................................................................................................................... |
4-17 |
Table |
4-3 |
: Waste bin filling level icons .................................................................................................................. |
4-18 |
Table |
4-4 |
: Sample processing status icons........................................................................................................... |
4-26 |
PREVITM Isola User Manual |
III-1 |
1 How to use this Manual
This manual provides the appropriate instructions to install, operate and maintain the
PREVI Isola.
CAUTION! The user is advised to read and understand all the instructions in this manual to be able to derive the best performance from the PREVI Isola.
F i n d i n g t o p i c s a n d p r o c e d u r e s
|
This manual is divided into 12 chapters. |
Table of contents |
The Table of Contents of the manual is located on pages I-1 to I-6. It lists each chapter and |
|
the procedures and/or topics contained in the chapter. |
List of figures |
Pages II-1 to II-4 contain a list of the figures in this manual. |
Page headers and |
Apart from the first page of every chapter, each page of this manual includes a page header |
page footers |
and a footer. |
|
Each page header includes the chapter title and the title of a procedure or its corresponding |
|
description. |
|
The footers contain the name of the product, the title of the manual, and a page number. |
Glossary |
The glossary is located in Chapter 12. It gives the definition of the main technical terms used |
|
in the manual. |
W a r n i n g s
Different types of warnings are used throughout the manual:
− For safety reasons (DANGER!).
− To ensure that the instrument is maintained in good working condition (CAUTION!).
− For regulatory reasons (WARNING!) or,
− For optimum performance of operations, procedures, etc. (IMPORTANT!).
Please also read the “General safety and regulatory information” booklet provided with the instrument.
PREVITM Isola User Manual |
1-1 |
2 Description
I n t e n d e d U s e
The PREVI Isola is an automated device intended to perform the inoculation and streaking of Prepoured Media (PPM) with liquid microbiological samples.
S y s t e m C a p a b i l i t i e s
The PREVI Isola has been designed to allow the user:
−to load between 1 and 114 samples for processing,
−to load up to 150 plates.
The PREVI Isola:
−provides automated PPM inoculation,
−provides automated streaking of PPM,
−delivers 180 plates per hour of operation, based on a protocol of 2 plates/sample,
−ensures there is no possibility of cross-contamination during the process,
−is equipped with a PREVI Isola HEPA filter to avoid contamination in the laboratory during processing.
PREVITM Isola User Manual |
2-1 |
Description
Workflow Principle
W o r k f l o w P r i n c i p l e
Specimen collection
|
|
Specimen reception & |
|
Sample |
sample preparation |
|
Sample data |
|
|
|
|
|
|
LIS |
User |
|
Sample data |
Sample |
|
Configuration |
|
|
Laboratory Manager |
Sample data
Incubation
Figure 2-1 : Workflow Principle
2-2 |
PREVITM Isola User Manual |
Description
System Architecture
S y s t e m A r c h i t e c t u r e
PREVI Isola |
PREVI Isola |
PREVI Isola |
PREVI Isola |
Instrument 1 |
Instrument 2 |
Instrument 3 |
Instrument 4 |
Data Server
WORKSTATION
BCI
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
LIS |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Figure 2-2 : System Architecture |
||
|
LIS |
|
Laboratory Information System. |
|
|
BCI |
|
Bi-directional Computer Interface, a bridge to LIS. |
|
|
Workstation |
|
User interface application which runs on a PC that is separate |
|
|
|
|
from the instrument. |
|
|
Data Server |
|
The database holding amongst other things, the plate panel |
|
|
|
|
definitions, and the sample assignments. |
|
|
PREVI Isola |
|
The plate processing instrument. |
|
Note: |
Four PREVI Isola instruments can be connected to a workstation. |
|||
PREVITM Isola User Manual |
2-3 |
Description
System Components
S y s t e m C o m p o n e n t s
The main system components are:
−an instrument 
with an internal computer and a touch-screen
−a standalone computer - workstation 
−a UPS (optional)
−two types of cassettes (input 
, output 
)
−five types of sample racks 
−consumables (PREVI Isola Applicators, PREVI Isola Tips, PREVI Isola Labels,
PREVI Isola Waste Bins)
−a waste paper bin (for label backing) 
Figure 2-3 : System Components
2-4 |
PREVITM Isola User Manual |
Description
Workstation
W o r k s t a t i o n
Description
|
The workstation is composed of: |
|
|
− |
1 computer |
|
− |
1 screen |
|
− |
1 keyboard |
|
− |
1 mouse |
|
− |
1 barcode reader |
|
− |
1 printer (optional) |
Note: |
Since the PREVI Isola system operates on a Microsoft Windows OS, it can be connected to |
|
|
an external printer compatible with Microsoft Windows. |
|
To validate this printer, please perform the Printer Verification Protocol, page 6-14.
PREVITM Isola User Manual |
2-5 |
Description
Instrument
I n s t r u m e n t
Figure 2-4 : Front view of the PREVI Isola with covers on
Consumable lid
Provides access to the consumables tray for loading/unloading of tips and applicators.
Process station lid (nose cone and center cover).
Provides access to the processing area for wiping down the process stations.
Printer access door
Provides access for loading labels into the printer.
Output cassette guard
Sample lid
Provides access to the sample loading bay for loading/unloading of sample racks.
Waste drawer
CAUTION! The PREVI Isola contains moving mechanisms. Do not remove the process station lid while the instrument is in operation.
2-6 |
PREVITM Isola User Manual |
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