BioMerieux mini APT Service manual

mini API®

Service Manual

Product No. 99 819

Version C

01/2007

bioMérieux sa 69280 Marcy l'Etoile / France

Tel. [phone] - Fax [phone]

http://www.biomerieux.com

Printed in France / 673 620 399 RCS Lyon

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Distribution in over 130 countries

V.B 02/2005

Information supplied in this manual may be subject to modifications before
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®

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TABLE OF CONTENTS

LIST OF FIGURES.........................................................................................................V-1

1 HOW TO USE THIS MANUAL................................................................................. 1-1

1.1 Introduction..................................................................................................................................... 1-1

1.2 Scope of the manual ...................................................................................................................... 1-2

1.3 Finding topics and procedures ....................................................................................................... 1-3

1.4 How the manual is organised......................................................................................................... 1-4

2 FUNCTIONAL DESCRIPTION................................................................................. 2-1

2.1 Introduction..................................................................................................................................... 2-1

2.2 Presentation ................................................................................................................................... 2-2

2.3 Introduction to identification and susceptibility testing ................................................................... 2-3

2.4 Description/Aim .............................................................................................................................. 2-4

2.5 General description ........................................................................................................................ 2-5

2.5.1 Configuration components ...................................................................................................... 2-5

2.6 Principle of operation ..................................................................................................................... 2-7

2.6.1 Functions ................................................................................................................................ 2-7

2.6.2 Presentation............................................................................................................................ 2-7

2.6.3 Characteristics ........................................................................................................................ 2-7

2.7 General characteristics .................................................................................................................. 2-9

2.7.1 Environmental conditions........................................................................................................2-9

2.7.2 Physical features..................................................................................................................... 2-9

2.7.3 Electrical characteristics ....................................................................................................... 2-10

2.7.4 Characteristics of optical components .................................................................................. 2-10

2.8 Computer...................................................................................................................................... 2-11

2.9 Central processing unit ................................................................................................................ 2-11

2.10 Software ....................................................................................................................................... 2-11

2.11 Consumables ............................................................................................................................... 2-12

3 PRELIMINARY INSTRUCTIONS ............................................................................. 3-1

3.1 Recommendations for installation and use .................................................................................... 3-2

3.2 Unpacking the mini API................................................................................................................. 3-2

3.3 Assembly and installation............................................................................................................... 3-3

3.3.1 Choosing a location ................................................................................................................ 3-3

3.3.2 Setting up the strip tray ........................................................................................................... 3-5

3.3.3 Connections ............................................................................................................................ 3-5

3.3.4 Preparing for operation ........................................................................................................... 3-5

3.3.5 Adjusting the height of the mini API ......................................................................................... 3-5

mini API Service Manual IV-1

4 DESCRIPTION OF MODULES.................................................................................4-1

4.1 Introduction..................................................................................................................................... 4-1

OVERVIEW.....................................................................................................................4-3

4.2 Precautions..................................................................................................................................... 4-5

4.3 Tools 4-5

4.4 Disassembly and assembly ............................................................................................................ 4-6

mini API..........................................................................................................................4-7

4.5 Cable connections .......................................................................................................................... 4-8

4.6 Principle of operation.................................................................................................................... 4-10

4.6.1 Colorimetric reading .............................................................................................................. 4-10

4.6.2 Nephelometric reading .......................................................................................................... 4-11

4.7 Block diagram of analogue digital converter................................................................................. 4-12

4.7.1 Removing the casing (Casing ASSY) = 452 1040A .............................................................. 4-14

POWER SUPPLY BLOCK ...........................................................................................4-15

4.8 Cable connections ........................................................................................................................ 4-16

4.9 Equipment .................................................................................................................................... 4-17

4.10 Removing the power supply block (451 1324A) .......................................................................... 4-17

4.11 Board power (450 0628A) ............................................................................................................ 4-18

4.11.1 Location................................................................................................................................. 4-18

4.11.2 Removing the board power ................................................................................................... 4-18

4.12 Principle of operation (power supply block).................................................................................. 4-20

4.12.1 Power supply circuit operation .............................................................................................. 4-20

4.12.2 Reader power supply ............................................................................................................ 4-20

4.12.3 Computer power supply ........................................................................................................ 4-20

4.13 Principle of operation (board power) ............................................................................................4-21

4.13.1 Filter motor drive circuit ......................................................................................................... 4-21

4.13.2 Tray motor drive circuit.......................................................................................................... 4-22

4.14 Testing and adjustment ................................................................................................................ 4-23

4.14.1 Reader power supply ............................................................................................................ 4-23

4.14.2 Board power .......................................................................................................................... 4-24

4.14.3 Procedure.............................................................................................................................. 4-25

OPTICAL BLOCK.........................................................................................................4-27

4.15 Optical block cable connections ................................................................................................... 4-28

4.16 Equipment .................................................................................................................................... 4-29

4.17 Removing the optical block........................................................................................................... 4-29

4.18 Condenser (P/N 452 0409A) ........................................................................................................ 4-30

4.18.1 Removing the condenser ...................................................................................................... 4-30

4.19 Lamp (P/N 451 0293A)................................................................................................................. 4-31

4.19.1 Removing the Lamp .............................................................................................................. 4-31

4.19.2 Removing the lamp (cont'd) .................................................................................................. 4-32

4.20 Board optic acquisition (P/N 450 0631A)...................................................................................... 4-33

4.20.1 Location................................................................................................................................. 4-33

4.20.2 Removing the board optic acquisition ................................................................................... 4-33

4.21 Board detection tray (Sync Assy) (P/N 450 0630A) ..................................................................... 4-34

4.21.1 Location................................................................................................................................. 4-34

4.21.2 Removing the board detection tray ....................................................................................... 4-34

IV-2 mini API Service Manual

4.22 Board detection filter holder (P/N 450 0629A) ............................................................................. 4-35

4.22.1 Location ................................................................................................................................ 4-35

4.22.2 Removing the board..............................................................................................................4-35

4.23 Principle of operation ................................................................................................................... 4-36

4.23.1 Lamp states .......................................................................................................................... 4-36

4.23.2 Board optic acquisition.......................................................................................................... 4-37

4.23.3 Board detection tray.............................................................................................................. 4-38

4.23.4 Board detection filter holder .................................................................................................. 4-38

4.24 Testing and alignment.................................................................................................................. 4-39

4.24.1 Lamp and condenser ............................................................................................................ 4-39

4.24.2 Board optic acquisition.......................................................................................................... 4-43

4.24.3 Board detection tray.............................................................................................................. 4-44

MANAGEMENT BLOCK.............................................................................................. 4-47

4.25 Cable connections........................................................................................................................ 4-48

4.26 Equipment .................................................................................................................................... 4-49

4.27 Removing the board COM GEST2............................................................................................... 4-49

4.28 Principle of operation ................................................................................................................... 4-50

4.28.1 RS 232 serial communication ............................................................................................... 4-52

4.28.2 EPROM version communication ........................................................................................... 4-52

4.28.3 Checking the position of the protection rail ........................................................................... 4-52

4.28.4 Initialisation of the reader module ......................................................................................... 4-52

4.28.5 Reading mode....................................................................................................................... 4-52

4.28.6 Reading................................................................................................................................. 4-53

4.28.7 Alarm messages ................................................................................................................... 4-55

4.28.8 Management card .................................................................................................................4-57

BOARD INTERFACE SERIAL LCD CPU .................................................................... 4-59

4.29 Removing the board interface (450 0625A) ................................................................................. 4-61

4.30 Cable connections........................................................................................................................ 4-62

4.31 Principle of operation ................................................................................................................... 4-63

COMPUTER BLOCK WITH BOARD PCM4862 .......................................................... 4-65

4.32 Cable connections........................................................................................................................ 4-67

4.33 Central processing unit ................................................................................................................ 4-68

4.34 Board CPU PCM 4862 (450 0572A) ............................................................................................ 4-69

4.35 Introduction................................................................................................................................... 4-70

4.35.1 Safety precautions ................................................................................................................ 4-70

4.35.2 Removing the board CPU PCM 4862 (450 0572A) .............................................................. 4-70

4.36 Specification................................................................................................................................. 4-71

4.36.1 Standard SBC functions........................................................................................................ 4-71

4.37 Jumpers and connectors.............................................................................................................. 4-74

4.38 SIMM memory modules ............................................................................................................... 4-78

4.39 Detailed information on serial ports.............................................................................................. 4-79

4.39.1 RS-232 port connector (CN13) ............................................................................................. 4-79

4.39.2 RS-232/422/485 Serial Port (CN8) ....................................................................................... 4-80

4.40 Jumper settings............................................................................................................................ 4-81

mini API Service Manual IV-3

COMPUTER BLOCK WITH BOARD PCM4860...........................................................4-85

4.41 Cable connections ........................................................................................................................ 4-87

4.42 Central processing unit................................................................................................................. 4-88

4.43 Board CPU PCM 4860 ................................................................................................................. 4-89

4.44 Introduction................................................................................................................................... 4-90

4.44.1 Safety precautions................................................................................................................. 4-90

4.44.2 Removing the board CPU PCM 4860 ................................................................................... 4-90

4.45 Specifications ............................................................................................................................... 4-91

4.45.1 Standard SBC functions........................................................................................................ 4-91

4.46 Jumpers and connectors .............................................................................................................. 4-94

4.47 SIMM memory modules................................................................................................................ 4-97

4.48 Detailed information on serial ports .............................................................................................. 4-98

4.48.1 RS-232 port connector (JP14)............................................................................................... 4-98

4.48.2 RS-232/422/485 Serial Port (JP9)......................................................................................... 4-99

4.49 Jumper settings .......................................................................................................................... 4-100

4.50 Floppy drive ................................................................................................................................ 4-101

4.50.1 Features .............................................................................................................................. 4-101

4.50.2 Removing the floppy drive and hard disk ............................................................................ 4-101

4.51 Hard disk .................................................................................................................................... 4-102

4.51.1 Features .............................................................................................................................. 4-102

4.52 Display........................................................................................................................................ 4-104

4.52.1 Mechanical data .................................................................................................................. 4-104

4.52.2 Removing the display (451 1023A) ..................................................................................... 4-104

4.53 Cable connections ...................................................................................................................... 4-105

4.53.1 Electrical absolute maximum ratings................................................................................... 4-106

4.53.2 Environmental absolute maximum ratings .......................................................................... 4-106

4.53.3 Electrical characteristics of backlight .................................................................................. 4-107

4.53.4 Precautions for use ............................................................................................................. 4-107

4.53.5 Storage................................................................................................................................ 4-107

4.53.6 Safety .................................................................................................................................. 4-107

PRINTER BLOCK.......................................................................................................4-109

4.54 Outline ........................................................................................................................................ 4-111

4.54.1 Removing the printer ........................................................................................................... 4-111

4.55 Part functional description .......................................................................................................... 4-112

4.56 General specifications ................................................................................................................ 4-113

4.57 Connectors and signals .............................................................................................................. 4-115

4.58 Setting of DIP switches............................................................................................................... 4-116

4.59 mini API printer setting .............................................................................................................. 4-117

4.60 Printer interface box (451 1024a) ............................................................................................... 4-117

4.61 Printer cable connections ........................................................................................................... 4-118

IV-4 mini API Service Manual

5 PREVENTIVE MAINTENANCE ............................................................................... 5-1

5.1 Introduction..................................................................................................................................... 5-1

5.2 Testing the mini API ...................................................................................................................... 5-2

5.3 Preventive maintenance................................................................................................................. 5-3

5.3.1 Cleaning the mini API®........................................................................................................... 5-3

5.3.2 Changing the ribbon................................................................................................................ 5-9

5.3.3 Changing the roll of paper..................................................................................................... 5-10

5.4 Changing the fuse ........................................................................................................................ 5-12

5.5 Field engineer preventive maintenance ....................................................................................... 5-13

6 TROUBLESHOOTING GUIDE................................................................................. 6-1

6.1 Introduction..................................................................................................................................... 6-1

6.2 Overview ........................................................................................................................................ 6-2

6.2.1 Determining the problem.........................................................................................................6-2

6.2.2 Solving the problem ................................................................................................................ 6-2

6.3 Error message troubleshooting ...................................................................................................... 6-3

6.3.1 Error messages appearing in the mini API software.............................................................. 6-3

6.3.2 Reference alarm ..................................................................................................................... 6-4

6.4 Repair by unit replacement ............................................................................................................ 6-6

6.4.1 Flow chart (1) abnormal operation at power on ...................................................................... 6-7

6.4.2 Flow chart (2) abnormal strip tray operation ........................................................................... 6-8

6.4.3 Flow chart (3) incorrect data sent to the computer ................................................................. 6-9

7 MODULE SPARE PARTS........................................................................................ 7-1

7.1 Introduction..................................................................................................................................... 7-1

7.2 List of tools ..................................................................................................................................... 7-2

7.3 Electronic board ............................................................................................................................. 7-2

7.4 Electrical parts................................................................................................................................ 7-3

7.5 Mechanical parts ............................................................................................................................ 7-4

7.6 Cables ............................................................................................................................................ 7-4

8 DIAGRAMS .............................................................................................................. 8-1

8.1 Introduction..................................................................................................................................... 8-1

9 APPENDIX - MAINTENANCE SOFTWARE .......................................................... 9-13

9.1 Introduction................................................................................................................................... 9-13

9.2 Getting started.............................................................................................................................. 9-14

9.3 Overview ...................................................................................................................................... 9-15

9.3.1 Function "[F4] Filter Values" ................................................................................................. 9-15

9.3.2 Function "[F1] Config" ........................................................................................................... 9-15

9.3.3 Function "[F2] Adjustment" ................................................................................................... 9-16

9.3.4 Function "[F3] Reading" ........................................................................................................ 9-27

10 GLOSSARY ........................................................................................................ 10-1

11 INDEX.................................................................................................................. 11-1

mini API Service Manual IV-5

LIST OF FIGURES

Page

2. FUNCTIONAL DESCRIPTION

Figure 2–1: Using mini API®............................................................................................2-3

Figure 2–2: Description of the spectral zone ....................................................................2-6

Figure 2–3: Optics layout.................................................................................................. 2-6

Figure 2–4: Filter wheel .................................................................................................... 2-6

Figure 2–5: Configuration components.............................................................................2-8

3. PRELIMINARY INSTRUCTIONS

Figure 3–1: Setting up the strip tray.................................................................................. 3-4

Figure 3–2: Connections...................................................................................................3-4

4. DESCRIPTION OF MODULES

Figure 4–1: Cable connections ......................................................................................... 4-8

Figure 4–2: Calculation of numerical values .....................................................................4-9

Figure 4–3: Transmission graph ..................................................................................... 4-10

Figure 4–4: Filter wheel .................................................................................................. 4-10

Figure 4–5: Analogue-digital converter........................................................................... 4-12

Figure 4–6: Position of casing screws ............................................................................ 4-13

Figure 4–7: Power supply cable connections .................................................................4-16

Figure 4–8: Removing the power supply block ...............................................................4-17

Figure 4–9: Removing the board power .........................................................................4-18

Figure 4–10: Power supply circuit block diagram ...........................................................4-19

Figure 4–11: Filter motor drive circuit ............................................................................. 4-21

Figure 4–12: Tray motor drive circuit .............................................................................. 4-22

Figure 4–13: Switching power supply card testing and adjustment ................................ 4-23

Figure 4–14: Board power .............................................................................................. 4-24

Figure 4–15: Detection of the tray dt = 200ms (tray speed)............................................ 4-25

Figure 4–16: Optical block cable connections ................................................................4-28

Figure 4–17: Removing the optical block........................................................................4-29

Figure 4–18: Removing the condenser........................................................................... 4-30

Figure 4–19: Removing the lamp.................................................................................... 4-31

Figure 4–20: Handling the lamp...................................................................................... 4-32

mini API Service Manual V-1

Figure 4–21: Removing the board optic acquisition ....................................................... 4-33

Figure 4–22: Removing the board detection tray ........................................................... 4-34

Figure 4–23: Removing the board detection filter holder................................................ 4-35

Figure 4–24: Lamp control drive circuit .......................................................................... 4-36

Figure 4–25: Analogical switching.................................................................................. 4-37

Figure 4–26: Board detection tray circuit........................................................................ 4-38

Figure 4–27: Board detection filter holder circuit............................................................ 4-38

Figure 4–28: Beam alignment ........................................................................................ 4-39

Figure 4–29: Strip code reading..................................................................................... 4-41

Figure 4–30: Oscillogram and holes on the tray............................................................. 4-41

Figure 4–31: Position of the beams ............................................................................... 4-43

Figure 4–32: Alignment of the board detection tray ....................................................... 4-45

Figure 4–33: Management block cable connections ...................................................... 4-48

Figure 4–34: Removing the board COM GEST2............................................................ 4-49

Figure 4–35: Management block diagram...................................................................... 4-51

Figure 4–38: Removing the board interface ................................................................... 4-60

Figure 4–39: Board interface serial LCD CUP cable connections.................................. 4-62

Figure 4–40: Computer cable connections..................................................................... 4-67

Figure 4–41: Removing the board CPU ......................................................................... 4-69

Figure 4–42: PCM-4862 jumpers ................................................................................... 4-73

Figure 4–43: PCM-4862 connectors .............................................................................. 4-76

Figure 4–44: PCM-4862 BIOS Settings ......................................................................... 4-83

Figure 4–45: Computer cable connections..................................................................... 4-87

Figure 4–46: Removing the board CPU ......................................................................... 4-89

Figure 4–47: PCM-4860 jumpers ................................................................................... 4-93

Figure 4–48: PCM-4860 connectors .............................................................................. 4-95

Figure 4–49: Display .................................................................................................... 4-103

Figure 4–50: Display cable connections....................................................................... 4-105

Figure 4–51: Printer ..................................................................................................... 4-110

Figure 4–52: Power supply connector.......................................................................... 4-115

Figure 4–53: DIP switch setting ................................................................................... 4-117

Figure 4–54: Printer cable connections........................................................................ 4-118

Figure 4–55: Printer interface circuit ............................................................................ 4-119

V-2 mini API Service Manual

5. PEVENTIVE MAINTENANCE

Figure 5–1: Strip tray ........................................................................................................ 5-4

Figure 5–2: Preparing the photodiode cleaning tool ......................................................... 5-6

Figure 5–3: Cleaning the photodiodes.............................................................................. 5-6

Figure 5–4: Changing the ribbon and the roll of paper .....................................................5-8

Figure 5–5: Printer cover .................................................................................................. 5-8

Figure 5–6: Paper cutter................................................................................................... 5-9

Figure 5–7: Loading the paper........................................................................................ 5-11

Figure 5–8: Back panel of the mini API .........................................................................5-12

6. TROUBLESHOOTING

Figure 6–1: Optical interrupt of the tray ............................................................................ 6-5

TABLES

Table A: Recommended tools ..........................................................................................4-5

Table B: Equipment required for maintenance .................................................................4-6

Table C: Limits................................................................................................................4-42

Table D: Symptom and flow chart references................................................................... 6-6

mini API Service Manual V-3

1 HOW TO USE THIS MANUAL

1.1 INTRODUCTION

This chapter explains how to use the mini API® Service Manual.

It is structured as follows:

SCOPE OF THE MANUAL.......................................................................... 1-2 ♦

♦

FINDING TOPICS AND PROCEDURES..................................................... 1-3

♦

HOW THE MANUAL IS ORGANISED.........................................................1-4

mini API Service Manual 1-1

1.2 SCOPE OF THE MANUAL

The mini API® Service Manual is designed to enable the Field Service Engineers
involved in maintenance tasks to accurately diagnose problems in order to repair
the mini API.

This chapter explains how the manual is organised and how it should be used.

The Table of Contents lists all the chapters the manual deals with. Each chapter is
subdivided into sections corresponding to a procedure or a precise description.

The reply to a question can readily be found by referring to the Table of Contents
or the Index at the back of the manual.

1-2 mini API Service Manual

1.3 FINDING TOPICS AND PROCEDURES

The mini API® Service Manual is divided into 12 chapters to help you find what
you are looking for.

Table of contents

The main table of contents of the manual is located on pages IV-1 to IV-5. It
lists each chapter and the procedures or topics they contain.

List of figures

Pages V-1 to V-3 contain a list of the figures of the manual.

Chapter contents

Each chapter starts with a specific table of contents. Each table of contents
shows the page from which the different sub-chapters start, on the right of
the page.

Page footers

Each page of the manual has a page footer.

The footers contain the title of the manual and the page number.

Index

A standard topical index is located at the back of the manual in chapter 11. It
is used to locate a particular description or procedure.

mini API Service Manual 1-3

1.4 HOW THE MANUAL IS ORGANISED

This manual is designed to provide you with step-by-step procedures for proper
maintenance of your mini API

®

.

Use this Service Manual when you want background or more detailed information
on the mini API components.

This chapter explains how the Service Manual is organised and how it should be
used.

FUNCTIONAL DESCRIPTION

Chapter 2 gives an overview of the mini API.

− Instrument functions

− Instrument operating principle

− Characteristics and specifications

PRELIMINARY INSTRUCTIONS

Chapter 3 describes the installation procedure and the procedure for
preliminary controls.

DESCRIPTION OF THE MODULES

Chapter 4 gives for each module:

− Its function.

− The position on the instrument.

− A block diagram.

− Diagrams and technical drawings.

− Procedures allowing you to assemble/disassemble the module.

− Procedures to maintain and repair the instruments.

PREVENTIVE MAINTENANCE

Chapter 5 describes the customer's schedule for maintenance as well as the
ATI maintenance.

TROUBLESHOOTING GUIDE

Chapter 6 describes all possible failures and gives the decision tree.

1-4 mini API Service Manual

MODULE SPARE PARTS

Chapter 7 gives the list of specific tools required to perform the adjustments
and carry out repairs on the instruments.

It also gives you the list of spare parts.

DIAGRAMS

Chapter 8 gives you the block diagrams for the mini API

®

boards.

MAINTENANCE SOFTWARE

Chapter 9 contains the description of the maintenance software used for the
reader.

GLOSSARY

Chapter 10 contains an alphabetical list of terms used in this manual and
their definitions.

INDEX

A standard topical index is located at the back of the manual in chapter 11.

mini API Service Manual 1-5

2 FUNCTIONAL DESCRIPTION

2.1 INTRODUCTION

The following topics are presented in this chapter:

PRESENTATION......................................................................................... 2-2 ♦

♦

INTRODUCTION TO IDENTIFICATION AND

SUSCEPTIBILITY TESTING ....................................................................... 2-3

♦

DESCRIPTION............................................................................................ 2-4

♦

GENERAL DESCRIPTION.......................................................................... 2-5

- Configuration components

- Hardware

- Software

- Consumables

♦

PRINCIPLE OF OPERATION .....................................................................2-7

- Functions

- Presentation

- Characteristics

♦

GENERAL CHARACTERISTICS................................................................. 2-9

- Environmental conditions

- Physical features

- Electrical characteristics

- Characteristics of optical components

♦

COMPUTER.............................................................................................. 2-11

♦

CENTRAL PROCESSING UNIT................................................................ 2-11

♦

SOFTWARE .............................................................................................. 2-11

♦

CONSUMABLES .......................................................................................2-12

mini API Service Manual 2-1

2.2 PRESENTATION

The mini API® is an automatic system for bacterial identification and susceptibility
testing. It can be divided into four categories of elements:

 hardware

 software

 consumables

 user's manual

2-2 mini API Service Manual

A

TM

A

2.3 INTRODUCTION TO IDENTIFICATION AND SUSCEPTIBILITY TESTING

Isolation medium

orientation tests

utomated technique

Standardised
inoculation using
the electronic
pipette

Standardisation

of the inoculum

using the

DENSIMAT

Choice of strips

Manual technique

ID 32 or rapid ID 32 strips ATB

utomatic reading

and interpretation.

or rapid ATB strips

mini API

Visual reading.

Entry of biochemical

profiles via the keyboard.

Automatic interpretation.

Figure 2–1: Using mini API®

mini API Service Manual 2-3

2.4 DESCRIPTION/AIM

The mini API® is designed for the automatic identification and susceptibility testing
of ID 32, rapid ID 32, ATB

It also enables computer assisted interpretation of API

TM

and rapid ATB strips.

®

strips read visually.

2-4 mini API Service Manual

2.5 GENERAL DESCRIPTION

2.5.1 Configuration components

The mini API® is an automated instrument for identification and susceptibility
testing. It consists of:

− hardware,

− software,

− consumables.

2.5.1.1 Hardware

The package includes:

 The mini API, a self-contained analytical module enabling:

− reading of test strips,

− management of results and data,

− printing of results obtained.

 a DENSIMAT densitometer to standardise the turbidimetry of the bacterial

suspension (please refer to the manual supplied with the densitometer).

 an electronic pipette to dispense the required amount of bacterial suspension

into each cupule of the identification and susceptibility test strips (55 µl or
135 µl) (please refer to the manual supplied with the pipette).

2.5.1.2 Software

The mini API software enables:

− interpretation of data,

− interpretation of strip results (identification or susceptibility tests),

− expert analysis of susceptibility tests,

− storage of results on the hard disk,

− printing of results,

− extraction of data,

− connection with a central computer.

2.5.1.3 Consumables

The mini API consumables include:

− strips with associated media and reagents

− ribbons and paper for the printer,

− pipette tips.

(see package inserts),

mini API Service Manual 2-5

transmission %

Wavelength in mm

Figure 2–2: Description of the spectral zone

Figure 2–3: Optics layout

Figure 2–4: Filter wheel

2-6 mini API Service Manual

2.6 PRINCIPLE OF OPERATION

2.6.1 Functions

− Strip identification by strip decoding

− Two types of readings:

− turbinephelometric

− colorimetric

− Communication with the computer.

2.6.2 Presentation

− Black strip carriage.

− Blue protection rail.

2.6.3 Characteristics

− Description of the spectral zone.

− Reading principle of the test strip reader:

 Turbinephelometric reading

Turbinephelometric reading is used for test strips containing assimilation tests:

Example: ID 32 GN, ID 32 C, ATB UR.

A turbinephelometric reading cycle occurs in two stages:

1- Entry of the strip carriage.

Detection of the strip code.

2- Measurement without a filter.

Exit of the strip carriage.

At the end of the cycle, the result is transmitted to the computer.

 Colorimetric reading

Colorimetric reading is used for strips containing chromogenic substrates:

Example: ID 32 STAPH, rapid ID 32 A, rapid ID 32 E

A colorimetric reading cycle occurs in 4 stages:

1- 1st entry of the strip carriage:

Detection of the strip code,
Measurement under a K60 filter.

2- 1st exit of the strip carriage:

Measurement under a K40 filter.

3- 2nd entry of the strip carriage:

Measurement under a DTb (blue) filter.

4- 2nd exit of the strip carriage:

Measurement under a DTv (green) filter.

At the end of the reading cycle, the result is transmitted to the computer.

mini API Service Manual 2-7



1- mini API®
2- Keyboard
3- Electronic pipette
4- DENSIMAT Densitometer

Figure 2–5: Configuration components







2-8 mini API Service Manual