bioMerieux Air IDEAL 3P User Manual

Air IDEAL® 3P

User's Manual

161150-208 – I – en - 2012/10 — P/N 161150-208

LCB 71260 La Salle FRANCE

bioMérieux SA Chemin de l'Orme

69280 Marcy-l'Étoile - France

RCS Lyon 673 620 399 Tel. 33 (0)4 78 87 20 00 Fax 33 (0)4 78 87 20 90

www.biomerieux.com

Revisions

The list of revisions below summarizes replacements or additional pages in your

Air IDEAL 3P User’s Manual.

Version

A 01/2001 Bilingual

B 04/2001 Multilingual

C 11/2005 Update of the manual

D 03/2006 Update of the manual

E 03/2008 Environmental conditions

F - Not used -

G - Not used -

Date of

printing

Modifications Page (s) modified

All

(FR / EN)

All

(FR / EN / ES / IT / DE / PT)

All

(FR / EN / ES)

All

(FR / EN / ES / IT / DE / PT)

2-12

(FR / EN / ES / IT / DE / PT)

H - Not used -

I 10/2012 Addition of figure "Diameter of Petri dishes".

Modification of section:

- "Characteristics of materials".

Modification of sections:

- "Cleaning and decontamination"

- "Decontamination of the external part".

Airflow control

3-5

3-7

5-1

5-2

Air IDEAL® 3P User’s Manual Revisions-1

4501-1618 B en

Warning

IMPORTANT! Use of the instrument and manual implies acceptance of the clauses below and the

The content of this manual is based on the Software release V2.

This manual is periodically updated. The updates shall be included in the new releases of the Software.

Information supplied in this manual may be subject to modifications before the products described become available.

This manual may contain information or references relating to certain bioMérieux SA products, software or services which are not available in the country of release; this shall not mean that bioMérieux SA intends to market such products, software or services in such country.

To request copies of publications or for any technical request, contact bioMérieux SA or your local distributor.

clauses set out in the regulatory booklet. Users are invited to refer to these clauses.

Trademarks

BIOMERIEUX, the blue logo, 3P, Air IDEAL 3P and Count-Tact are used, pending and/or registered trademarks belonging to bioMérieux, or one of its subsidiaries, or one of its companies.

ClearKlens IPA is a trademark belonging to Johnson Diversey.

Any other name or trademark is the property of its respective owner.

List of figures

Note: Screen captures and figures are given as examples only.

2. Functional description

Figure 2-1: Principle of the impaction biocollector.......................................................................................................2-1

3. Using Air IDEAL 3P

Figure 3-1: Identification of the instrument..................................................................................................................3-3

Figure 3-2: Front face .................................................................................................................................................3-3

Figure 3-3: Rear face ..................................................................................................................................................3-4

Figure 3-4: Keypad .....................................................................................................................................................3-4

Figure 3-5 : Diameter of Petri dishes ..........................................................................................................................3-5

Figure 3-6: Sampling head and grid – 90 mm Petri dishes .........................................................................................3-6

Figure 3-7: Tripod assembly .......................................................................................................................................3-6

Figure 3-8: Air IDEAL 3P in its carrying case............................................................................................................3-10

Figure 3-9: Sampling positions .................................................................................................................................3-12

Figure 3-10: Screwing on the sampling grid..............................................................................................................3-13

V-3 Air IDEAL

®

3P User’s Manual

1 How to use this manual

IMPORTANT! Please read the "General safety and regulatory information" booklet provided with the

instrument.

Finding topics and procedures

This manual is divided into 7 chapters.

Table of contents

The main table of contents of the manual is located on pages V-1 to V-2.

It lists each chapter and the procedures and/or topics contained in the manual.

List of figures

Page V-3 contains the list of figures found in this manual.

Warnings

Different types of warnings are used throughout the manual:

- for safety reasons (DANGER!),

- to ensure that the instruments are maintained in good working condition (CAUTION!),

- for regulatory reasons (WARNING!) or,

- for optimum performance of operations, procedures, etc. (IMPORTANT!).

Page headers and page footers

Apart from the first page of every chapter, each page of the manual includes a page header and a footer.

Each page header includes the chapter title and the title of a procedure or its corresponding description.

These titles are located on the outside of the page so that you can thumb through the pages to quickly locate a chapter or a procedure.

The footers contain the title of the manual, the name of the product and the page number.

Notes

This manual contains a certain number of notes that are used to emphasize a procedure or certain information.

Air IDEAL® 3P User’s Manual 1-1

How to use this manual

Glossary

ABS Acrylonitrile butadiene styrene.

Aerobiocollector or Air sampler

CFU

Instrument used to collect and count viable bacterial and fungal particles in a known and precise volume of air.

Number of Colony Forming Units read on the agar plate.

The number of CFU corresponds to the number of clusters that have grown on the agar.

Delayed start-up

MPN

Time between pressing the START button and the motor starting up.

Most Probable Number of microorganisms collected. Statistical correction of the CFU value (FELLER’s law).

NiMH Nickel Metal Hydride.

PVDF Polyvinylidene fluoride.

Ra coefficient

Roughness factor of a surface. It is the arithmetic mean of all profile deviations, positive or negative, compared to the mean line.

Sampling grid

Perforated and calibrated plastic device. The number of orifices, their diameter and arrangement, guarantee a CFU count (positive clusters) and a flow of air corresponding to the motor setting.

Time-delay 2 seconds required by the software to record a parameter.

1-2 Air IDEAL® 3P User’s Manual

Typographic conventions

These conventions are used in the different chapters of the manual.

How to use this manual

Typographic conventions

• Press

A bullet point is used to denote an action to be performed.

ABC 123

This typography is used to represent messages which appear on the display.

The Air IDEAL 3P keypad

There are 5 keys on the Air IDEAL 3P keypad. (See page 3-4 of the manual).

In this manual, the keys are referred to by their individual names, enclosed in angle brackets "< >".

Air IDEAL® 3P User’s Manual 1-3

A

2 Functional description

Presentation

“Pharmaceutical manufacturing evolves from an art to a science”.

This sentence alone from the FDA Guideline “Pharmaceutical cGMP for the 21st century – A Risk-Based Approach” summarizes the current revolution in the Pharmaceutical industry.

Conscious of these changes and remaining attentive to its customers, bioMérieux decided to improve its Air IDEAL 3P aerobiocollector in order to best respond to these new needs.

Air IDEAL 3P was thus developed and validated in order to provide a tool to the pharmaceutical industry that would guarantee a scientifically proven method of air sampling.

Air IDEAL 3P evidently remains perfectly suited to the enumeration of airborne microorganisms in less demanding work environments such as agribusiness.

In addition and in order to continue its universal application, Air IDEAL 3P is still available in two versions:

− one for the use of culture media in 90 mm diameter Petri dishes,

− the other designed for use with 65 or 70 mm plates.

The aspiration flow-rate of Air IDEAL 3P is calibrated at 100 L/min with an impact velocity of less than 20 m/s. According to good sterilization practices, the sampling grids can be sterilized in an autoclave, see section "Sterilization of grids" on page 5-1.

Operating principle

Air IDEAL 3P is an impaction aerobiocollector, used to detect the presence of viable

microorganisms in the environment to be tested by precise sampling of a given volume of air. Air is taken up with a turbine through a grid surface. The acceleration of airflow results in the impaction of airborne microorganisms on the agar. Passage of the air through the grid filters out particles, thereby facilitating the enumeration of CFU (colony forming units) after incubation of the medium.

A reading and statistical correction table is used to convert the number of CFU to the most probable number of microorganisms collected per m

ir inlet

Microorganisms

Impaction

Nutrient medium

3

of air.

Calibrated orifice

ir jets

90 or 65/70 mm diameter Petri dishes

ir outlet

Figure 2-1: Principle of the impaction biocollector

Air IDEAL® 3P User’s Manual 2-1

Functional description

Presentation

Performance

The performance characteristics of an aerobiocollector depend on its capacity to collect microorganisms in the air without compromising their viability during impaction on the agar. This property can be obtained only with a perfect compromise between the high aspiration velocity leading to effective collection, and a sufficiently low impaction velocity to guarantee the revivification of collected microorganisms.

Air IDEAL 3P was developed in close cooperation with aeraulics experts in order to optimize this ratio.

Since the industry has increasing needs for scientifically proven methods, bioMérieux commissioned two recognized independent organizations* biological efficiency of Air IDEAL 3P.

Collection efficiency validated according to the ISO 14698 standard

Air IDEAL 3P was third party validated by the Health Protection Agency (UK) to meet the

requirements of ISO 14698-1 for the control of clean rooms. Both the physical and biological efficiencies of the equipment have been validated according to this standard.

Physical efficiency testing approach

The physical efficiency of an air sampler for collecting airborne bacteria is evaluated by comparison with a membrane filter sampler. Uniform particles of different diameters containing bacterial spores of Bacillus subtilis var niger were generated in a controlled room. The physical efficiency of Air IDEAL 3P was determined by comparison with the membrane filtration standard operating side-by-side.

*

to validate the physical and

Biological efficiency testing approach

Air sampler inefficiency can either be due to a failure of the sampler to capture particles containing micro-organisms (physical loss), or to inactivation of viable micro-organisms during collection, so that formation of visible colonies on agar will not occur (biological loss).

To address this point, Air IDEAL 3P was evaluated for recovery of a mixture of Bacillus subtilis (standard indicator for physical loss) and Staphylococcus epidermidis (standard indicator for biological loss). The ratio of S. epidermidis / B. subtilis for the test samplers was divided by the ratio obtained with the reference standard membrane filter sampler to give a comparative biological efficiency.

Use in glove boxes

In order to be used to test glove boxes, the design and materials of Air IDEAL 3P had to be entirely reviewed in order to optimize system air tightness.

In addition and in order to guarantee the optimal operation of Air IDEAL 3P in this application, the system underwent a complete validation in a glove box (SKAN AG, model ARIS glove box).

Applications

Air IDEAL 3P enables the precise and reproducible sampling of air. The volumes taken can

be set in 10 L steps up to a maximum volume of 2000 L.

This sampling range enables Air IDEAL 3P to be used in all types of environments, from sterile zones to more contaminated surroundings and in all applications, e.g. qualification of sterile rooms or daily monitoring.

*

CETIAT: Centre Technique des Industries Aérauliques et Thermiques/Technical Center of Aeraulic and Thermal Industries

HPA: Health Protection Agency - Porton Down - Wiltshire SP4 0JG Salisbury - UK

Domaine Scientifique de la Doua, 69603 Villeurbanne, France

2-2 Air IDEAL® 3P User’s Manual

3 Using Air IDEAL 3P

Description

Air IDEAL 3P is supplied in a carrying case. Inside the case there is a rigid lid which can be

used as a small work surface [dimensions 22.7 x 13.9 cm (8.9 in. x 5.4 in.)].

Air IDEAL 3P is available in two versions:

− for 90 mm agar plates (product No. 96302)

− for 65/70 mm agar plates (product No. 96304)

 Each reference includes:

− an Air IDEAL 3P aerobiocollector fitted with a protective cover.

− 5 sampling grids

− an AC/DC charger-adapter (see page 3-9) for the internal battery.

− 4 jack protection caps.

− a hook for suspending the instrument.

− Documentation:

• User’s manual

• Product certificate

• Adjustment certificate

• Control certificate

Note: For product No. 96303 only, a stainless steel blade is supplied for use with 70 mm diameter Petri

dishes.

 Accessories:

− 65/70 mm diameter additional sampling grid (product No. 96304)

− 90 mm diameter additional sampling grid (product No. 96309)

− Aluminum telescopic tripod with ball joint (product No. 96308)

− Sterile box for transport and incubation of 65 mm plates – Kit of 10 (product No. 96301)

*

− Sterile box for transport and incubation of 90 mm plates – Kit of 10 (product No. 96311*

)

*

Availability: consult bioMérieux

Air IDEAL® 3P User’s Manual 3-1

Using Air IDEAL 3P

Description

Consumables

Air IDEAL 3P is adapted to all types of Petri dishes available on the market: 65, 70 and

90 mm.

Moreover, the use of a large range of ready-to-use culture media – irradiated or classic – developed and manufactured by bioMérieux, the reference in this field, enables you to obtain the best possible performance from Air IDEAL 3P.

The media comply with specific industrial and hospital environmental controls.

Culture media for general use

− 20 TSA 90 mm plates (product No. 43011)/100 TSA 90 mm (ref. 43019)

− 20 PCA agar 90 mm plates (product No. 43558)

− 20 Sabouraud dextrose agar 90 mm plates (product No. 43555)

− 20 Sabouraud dextrose chloramphenicol agar 90 mm plates (product No. 43596)

− 20 count-Tact agar 65 mm plates (product No. 43501)

− 20 count-Tact TSA agar 65 mm plates (product No. 43582)

− 20 count-Tact Sabouraud dextrose chloramphenicol agar 65 mm plates (product No.

43580)

Irradiated media

− 100 TSA irradiated 90 mm plates (product No. 43557)

− 20 TSA irradiated 90 mm plates (product No. 43131)

− 100 TSA with neutralizing agents 90 mm plates (product No. 43562)

− 20 Sabouraud dextrose irradiated agar 90 mm plates (product No. 43554)

− 20 Sabouraud dextrose chloramphenicol irradiated agar 90 mm plates (product No.

43595)

− 20 count-Tact irradiated agar 65 mm plates (product No. 43491)

− 100 count-Tact irradiated agar 65 mm plates (product No. 43492)

− 20 count-Tact Sabouraud dextrose chloramphenicol agar 65 mm plates (product No.

43581)

− 20 count-Tact 3P irradiated agar 65 mm plates (product No. 43691)

− 100 count-Tact 3P irradiated agar 65 mm plates (product No. 43699)

3-2 Air IDEAL® 3P User’s Manual

Identification of the instrument

Front face of Air IDEAL 3P

Using Air IDEAL 3P

Description

Figure 3-1: Identification of the instrument

Figure 3-2: Front face

Air IDEAL® 3P User’s Manual 3-3

Using Air IDEAL 3P

A

Description

Rear face of Air IDEAL 3P

Handle

ir outlet

Threading for tripod

Keypad

Jack socket

Figure 3-3: Rear face

Figure 3-4: Keypad

3-4 Air IDEAL® 3P User’s Manual

Configuration for 65, 70 or 90 mm Petri dishes

Using Air IDEAL 3P

Description

Sampling grid for 65, 70 mm Petri dishes

Count-Tact® agar plate

Sampling grid for 90 mm Petri dishes

90 mm Petri dish

Figure 3-5 : Diameter of Petri dishes

Air IDEAL® 3P User’s Manual 3-5

Using Air IDEAL 3P

Description

Stainless steel strip for attachment

Seal

Aspiration

orifice

Tripod assembly

Figure 3-6: Sampling head and grid – 90 mm Petri dishes

Figure 3-7: Tripod assembly

3-6 Air IDEAL® 3P User’s Manual

General specifications

Environmental conditions

Normal storage conditions............................................................................-20° to + 50°C

Inside use in an air class zone, sterile room or glove box.

Normal conditions of use ......................................................... 0 to 40 °C (32 °F to 104 °F)

Relative humidity ..................................................................................................0

Maximum installation altitude.............................................................................. < 2 000 m

Standards applicable ....................................................................... See product certificate

The instrument must be transported in its specific case.

Physical features

Dimensions

Instrument alone Instrument in its case

Height 128 mm

Width 146 mm

Depth 208 mm

(5.03 in.) 301 mm (11.8 in.) (5.75 in.) 250 mm (9.84 in.) (8.18 in.) 400 mm (15.75 in.)

Using Air IDEAL 3P

to 95%

Mass

Instrument alone Instrument in its case Shipping carton

≈ 1,3 kg ≈ 5,6 kg ≈ 7.1 kg

Materials

∗ ABS shell ∗ Polycarbonate keypad ∗ PVDF sampling grid ∗ Stainless steel mounting strips and screws ∗ Elastomer jack protection caps

Characteristics of materials

− Shock resistance: shell and keypad.

− Chemical resistance: to most standard disinfectants (70% isopropanol, hydrogen

peroxide, 70% ethanol, quaternary ammoniums).

− Thermal resistance: sampling grid that can be autoclaved using Good Sterilization Practices (18 minutes at 134°C in water saturated vapor).

Note: With increasing numbers of autoclaving cycles, the grids become increasingly yellow-gray, but this has

no effect on grid performance.

− Surface finish: Ra coefficient of grid = 0.14 micron.

− Fire resistance: ABS V0 shell (highest grade of fire resistance).

Air IDEAL® 3P User’s Manual 3-7

Using Air IDEAL 3P

General specifications

Technical characteristics

Keypad

− Key control.

Display

− Liquid crystal display (2 x 16 characters).

Sampling grid

− 1/8

− Possibility of sampling on 90 mm 65/70 mm diameter Petri dishes (specific sampling

grids and fixing strips).

User interface

− 5 buttons for access to all Air IDEAL 3P functions.

− An LCD screen is used to program the instrument and follow its operation. Messages are

in English.

− Additional information on instrument operation is given as audible signals.

th

of aturn screw-on sampling grid.

Ergonomics

− Ergonomic handle (right hand/left hand).

− Possibility of stable sampling with the unit in four positions (see page 3-12).

− Possibility of suspending Air IDEAL 3P with a hook, especially in a glove box.

− Optionally, Air IDEAL 3P can be mounted on a telescopic tripod equipped with a ball

joint enabling the sampling axis to be orientated through an angle of 0° to 90° (from horizontal to vertical), and its height to be adjusted (between 0.7 and 2.50 meters).

3-8 Air IDEAL® 3P User’s Manual

Electrical characteristics

Voltage ...................................................................................... 7.2 VDC nominal

Power supply.......................................................................................... 1 A max.

Air IDEAL 3P is designed to run on a battery (6 NiMH batteries connected in series, each with a nominal voltage of 1.2 V).

Air IDEAL 3P can also be powered and/or recharged using one of the adapters indicated or an equivalent one.

CAUTION! The AC-DC power supply adapter must have the following characteristics:

- Voltage: 12 VDC

- Power: 1.5 A max.

- Plug mod. Jack 12.0 x 2.1 mm

-

AC input voltage corresponding to the characteristics of the power supply in the

country where the instrument is installed.

Using Air IDEAL 3P

General specifications

Note: It is recommended to use the adapter provided with Air IDEAL 3P. You will find the details of its

characteristics below.

POWER SUPPLY UL/CSA

INPUT............................................ 120 VAC/60Hz 0.8 A max. USA STD plug

OUTPUT....................................................... 12 VDC 1.5 A max. JACK plug.

POWER SUPPLY complying with standards

INPUT................................... 230 VAC/50Hz 0.8 A max. European STD plug

OUTPUT....................................................... 12 VDC 1.5 A max. JACK plug.

Functional specifications

Flow-rate

− 100 ± 6.5 liters per minute regardless of the grid used.

− Flow-rate measured and adjusted according to the bioMérieux quality control protocol

reference 96302-protocol.

Autonomy

− More than or equal to 4 hours, enabling at least 24 consecutive 1000 l samples to be taken.

− Specification valid for a new battery not having undergone a thermal shock or a prolonged period of inactivity.

Charge time

It takes 14 hours to fully charge the battery.

Sound level

< 50 dB.

Security

Male plug outlet serves as the power supply sectioning device.

Air IDEAL® 3P User’s Manual 3-9

Using Air IDEAL 3P

Unpacking Air IDEAL 3P

The instrument is supplied in a carrying case and box.

 When opening the box:

• Make sure you have all the items described in the packing list.

• Keep the shipping carton in case you need to ship the instrument back to bioMérieux.

CAUTION! Any damage directly or indirectly resulting from the transport of the instrument

without adequate containers will not be covered by the warranty or maintenance contract.

DANGER ! Do not use the instrument in an explosive atmosphere as sparks could cause an

Packing list

explosion.

− an Air IDEAL 3P aerobiocollector fitted with a protective cover.

− 5 sampling grids

− a hook for suspending the instrument

− 4 jack protection caps

− an AC/DC charger-adapter (see page 3-9) for the internal battery.

− Documentation:

• User’s manual

• Product certificate

• Adjustment certificate

• Control certificate

Figure 3-8: Air IDEAL 3P in its carrying case

3-10 Air IDEAL® 3P User’s Manual

Recommendations for installation and use

• Install the instrument on a flat, perfectly horizontal surface, on its tripod or by its hook.

• Avoid locations directly exposed to sunlight, excessive heat, damp or dust.

• The instrument must not be used near strong sources of electromagnetic interference.

• Do not use the instrument with its original protective cover cap, but with a grid.

IMPORTANT! When the instrument is used for the first time, it is imperative to perform 1 battery

charge-discharge cycle.

•

Charge the battery for 14 hours.

•

Discharge the battery by performing successive sampling (see page 3-25

"Battery autonomy test (Menu 6)").

•

Recharge for 14 hours.

•

Make sure the grid holes are not blocked.

Using Air IDEAL 3P

Air IDEAL® 3P User’s Manual 3-11

Using Air IDEAL 3P

Sampling positions

 





Figure 3-9: Sampling positions

IMPORTANT! Air IDEAL 3P is delivered with a hook for suspending it in a glove box during

decontamination phases. Even though sampling can be done while suspended, it is preferable to position Air IDEAL 3P along the axis of one-way flow at the air outlets. This configuration is the worst case scenario since the air sampled has swept the entire volume of the glove box before emerging. Do not obstruct the air outlet during use to respect operating parameters.

3-12 Air IDEAL® 3P User’s Manual

Screwing on the sampling grid

CAUTION! Do not insert any foreign objects into the aspiration orifice located under the grid (see

pages 3-6).

 To screw on the sampling grid easily,

• Position the grid on the threading of the shell and turn it clockwise by 1/8

without forcing or pressing it.

Using Air IDEAL 3P

Screwing on the sampling grid

th

of a turn

Figure 3-10: Screwing on the sampling grid

Air IDEAL® 3P User’s Manual 3-13

Using Air IDEAL 3P

Electrical power supply

Air IDEAL 3P operates self-sufficiently through an internal nickel metal hybrid battery pack.

Air IDEAL 3P can also run connected to a charger/adapter.

Operation on internal battery

The microprocessor manages and displays the available autonomy at all times, on the basis

of a theoretical autonomy of 4 hours for sampling cycles of 1000 liters. In the case of

sampling cycles of less than 1000 liters, it is advisable to put the battery on charge as soon

Low battery signals

CAUTION! During this battery low phase, Air IDEAL 3P remains in its normal operating condition

as the flashing symbol appears:

.

 When the autonomy has expired, the following low battery signals are output:

Visual signal on the welcome message display:

Flashing symbol

Visual signal on the sample in progress display:

Flashing symbol

Audible signal each time the instrument is turned on: 2 long beeps

until the sampling cycle in progress is finished. Before performing a new sampling cycle, recharge the battery.

3-14 Air IDEAL® 3P User’s Manual

Charging

Using Air IDEAL 3P

Electrical power supply

 To charge the battery,

• Use the charger supplied or one with the same specifications.

• During the charging phase, Air IDEAL 3P can be either switched on or switched off.

• Connect the charger to the Air IDEAL 3P jack connector after removing the protection

cap.

• Connect the charger to the power outlet.

The display indicates:

The normal time required to completely recharge a discharged battery pack is 14 hours.

It is possible to perform sampling when the battery is being charged on the main power supply.

 At the end of the operation,

• Disconnect the charger from the power outlet.

• Disconnect the charger from the Air IDEAL 3P jack connector.

• Install the protection cap.

• Check that the available autonomy displayed is again 4 hours.

IMPORTANT! If Air IDEAL 3P is not used for more than 10 days, the battery must be totally

recharged (14 hours).

Operation using the power line

Connection to the power line is carried out in the same way as for charging the internal battery.

The display indicates:

• Press the <START> or <MENU> button to return to the welcome message.

Then

• Press the <START> button to start (the volume sampled will be the last recorded).

or,

• Select the sample volume by means of MENU 1 or MENU 2, and then start by pressing

the <START> button.

 To turn off,

• Disconnect the charger from the Jack socket of Air IDEAL 3P.

During operation on the power line, the internal battery is on charge.

IMPORTANT! During sampling with Air IDEAL 3P running off the main power supply, do not

disconnect the instrument from the main power supply as there is a risk of it switching off and the sampling cycle in progress would be definitively lost.

Automatic switch off

In order to save the available charge on the internal battery, the unit switches off automatically after 5 minutes of inactivity.

Air IDEAL® 3P User’s Manual 3-15

Using Air IDEAL 3P

Programming

Air IDEAL 3P enables samplings to be programmed and monitored.

This section describes how to program the instrument.

Programming characteristics

− Sample volumes adjustable from 5 to 10 liters in 1-liter steps.

− Sample volumes adjustable from 10 to 2000 liters in 10-liter steps.

− Automatic selection of last sample volume used.

− Storage of 4 sample volumes in the memory.

− Delayed start-up (maximum 60 minutes).

• 0 to 60 seconds in 1-second steps

• 1 minute to 60 minutes in 1-minute steps

− Sequenced sampling.

Sampling controls

− Volume counter.

− Volume of air sampled remains on display after sampling is interrupted.

− Possibility of interrupting and resuming a sample in progress.

− End of sample buzzer (6 short beeps).

− Count-down of time.

Additional information

− Symbol displayed and audible alarm (2 long beeps) at the end of battery autonomy.

− Automatic stop and message displayed if the protective cover was not removed.

− Display of remaining battery life (indicative value).

− Automatic switch-off of display after 5 minutes of inactivity.

− Language: English.

3-16 Air IDEAL® 3P User’s Manual

Air IDEAL 3P menus

Using Air IDEAL 3P

Programming

Turning on

WELCOME MESSAGE

Last sample taken. Remaining autonomy.

MENU 1:

Selection of one of 4 sample volumes among the 4 volumes preprogrammed in menu 3.

MENU 2: Programming 1 sample volume not in memory.

MENU 3: Modification of the 4 pre-programmed sample volumes.

MENU 4: Programming delayed start-up

MENU 5: Programming sequenced sampling.

MENU 6: Battery autonomy test.

Turning off

Air IDEAL® 3P User’s Manual 3-17

Using Air IDEAL 3P

Turning on Air IDEAL 3P

 To turn on Air IDEAL 3P,

• Press <ON/OFF>.

The display indicates:

Note: The symbol is displayed if a delayed start-up is programmed.

− ZZZL corresponds to the last sample volume recorded.

XHXX corresponds to remaining autonomy.

−

 If the symbol

• Recharge Air IDEAL 3P.

appears:

 If the last sample did not terminate correctly (sampling stopped before the end, protection

cap in place of the grid, motor blocked), the following is displayed:

In this case, press the <MENU> key to display the welcome message.

DANGER! Do not insert any foreign objects into the motor compartment when the instrument

is running.

Turning off Air IDEAL 3P

 To turn off Air IDEAL 3P,

• Press <ON/OFF>.

or

• Wait 5 minutes.

3-18 Air IDEAL® 3P User’s Manual

Selection of one of the 4 pre-programmed sample volumes (Menu 1)

 Factory programming memorizes the following 4 volumes in Air IDEAL 3P: 100, 500, 1000,

2000 liters.

 To select menu 1,

• Press <MENU>.

The display indicates:

• Press the < + > button to select 1 of the 4 volumes memorized.

Using Air IDEAL 3P

Turning off Air IDEAL 3P

Example:

 To select stored volume No. 2 from menu 1,

• Press < + > twice.

• Wait 2 seconds.

Note: If you did not wait for the end of the first timeout, in other words if you pressed another button before the

2 seconds, the new value selected (

YYY) is not recorded. The initial value (ZZZ) remains in memory.

The value YYY selected is recorded.

The display indicates:

 There are 3 possibilities:

• Start sampling immediately by pressing the <START> button.

• Wait: the selected sampling can be started at a later time.

• Press the <ON/OFF> button to turn off the instrument. The volume selected will be

displayed the next time it is turned on.

Air IDEAL® 3P User’s Manual 3-19

Using Air IDEAL 3P

Turning off Air IDEAL 3P

Programming a sample volume not in memory (Menu 2)

 To select menu 2,

• Press <MENU> twice.

The display indicates:

 To program a new volume,

• Press < + > or <- > until the desired volume is displayed.

IMPORTANT! It is imperative to press the

volume.

After a 2-second delay, the volume displayed is recorded.

The display indicates:

< + > or < - > buttons immediately to program a new

 There are 3 possibilities:

• Start sampling immediately by pressing <START>.

• Wait: the selected sampling can be started at a later time.

• Press the <ON/OFF> button to turn off the instrument. The volume selected will be

displayed the next time it is turned on.

Modification of the 4 pre-programmed sample volumes (Menu 3)

 To select menu 3,

• Press <MENU> 3 times.

The display indicates:

• Wait 2 seconds.

The display indicates:

3-20 Air IDEAL® 3P User’s Manual

Using Air IDEAL 3P

Turning off Air IDEAL 3P

To program a new volume for program 1,



• Press < + > or <- > until the desired volume is displayed.

IMPORTANT! It is imperative to press the

volume.

After a 2 second delay, the volume displayed is recorded.

The display indicates:

Note: If the <MENU>, < + > or < - >, buttons are not pressed, each of the 4 pre-programmed volumes is

displayed every 2 seconds.

< + > or < - > buttons immediately to program a new

 To program a new volume of program 2,

• Press < + > or < - > until the desired volume is displayed.

IMPORTANT! It is imperative to press the < + > or < - > buttons immediately to program a new

volume.

After a 2-second delay, the volume displayed is recorded.

The display indicates:

 To program a new volume for program 3,

• Press < + > or < - > until the desired volume is displayed.

IMPORTANT! It is imperative to press the

volume.

After a 2-second delay, the volume displayed is recorded.

The display indicates:

< + > or < - > buttons immediately to program a new

Air IDEAL® 3P User’s Manual 3-21

Using Air IDEAL 3P

Turning off Air IDEAL 3P

 To program a new volume for program 4,

• Press < + > or < - > until the desired volume is displayed.

IMPORTANT! It is imperative to press the

volume.

< + > or < - > buttons immediately to program a new

After a 2-second delay, the volume displayed is recorded.

The display indicates:

The screen the returns to the welcome message.

Note: If the < + > or < - > buttons are not pressed, the pre-programmed volumes are sequentially displayed

without modification.

 Possibility of going directly to "MENU 4", once selected volumes are modified:

END" is displayed on the screen,

When "

• Immediately press <MENU>.

Menu 4 is displayed.

3-22 Air IDEAL® 3P User’s Manual

Programming delayed start-up (Menu 4)

 Delayed start-up can be programmed for up to 60 minutes:

− 0 to 60 seconds in 1-second steps

− 1 minute to 60 minutes in 1-minute steps.

 To select menu 4,

• Press <MENU> 4 times.

The display indicates:

 To program a delay value,

• Press < + > or < - > until the desired delay value is displayed.

Using Air IDEAL 3P

Turning off Air IDEAL 3P

IMPORTANT! It is imperative to immediately press

After 2-seconds, the delay value is recorded.

The display indicates:

The screen returns to the welcome message.

The delay is recorded: the symbol

 There are 3 possibilities:

• Start sampling immediately by pressing <START>.

• Wait: the selected sampling can be started at a later time.

• Press the <ON/OFF> button to turn of the instrument. The volume selected will be

displayed the next time it is turned on.

< + > or < - > to program a new volume.

appears in the welcome message.

Air IDEAL® 3P User’s Manual 3-23

Using Air IDEAL 3P

Turning off Air IDEAL 3P

Programming sequenced sampling (Menu 5)

Menu 5 is used to sample a selected volume several times.

 This sequencing is defined with 3 parameters:

− The unit volume (of each sequence). This volume is defined with menus 1 and 2 (see pages 3-19 and 3-20).

− The number of sequences. This number is included between 2 and 5.

− The time interval between each sequence. It is included between 10 minutes and 4 hours, in 10-minute steps.

 Total sampling time must be less than 5 hours or else an error message appears.

 To select menu 5,

• Press <MENU> 5 times.

st

The following message appears and the 1

line flashes:

The unit volume is shown on the lower left and cannot be modified in this menu.

 To modify the number of sequences,

• Press < + > or < - > until the desired number is displayed.

• Press <MENU> once the number of sequences is selected.

The "INT X HXX" (INTERVAL ) field flashes.

• Press < + > or < - > to define the time interval between each sequence.

IMPORTANT! Check that the remaining battery autonomy is sufficient to carry out complete

sampling (base: 10 min/1000 liters).

3-24 Air IDEAL® 3P User’s Manual

Using Air IDEAL 3P

Turning off Air IDEAL 3P

 Once sequenced sampling has been correctly programmed,

• Press the <START> button to start sampling.

The total duration of sequences and intervals must not exceed 5 hours or else an error message appears:

Then, the screen automatically returns to Menu 5.

At this time, parameters (number of sequences/duration of the interval) must be corrected in order to enable sampling.

IMPORTANT! A sequenced sampling is run from the specific menu, without returning to the main

menu.

If the last sampling was sequenced, the menu 5 screen is displayed when Air IDEAL 3P is turned on.

Battery autonomy test (Menu 6)

This menu is used to verify that the battery is still compliant with specifications of new material (autonomy ≥ 4 hours).

The battery autonomy test can be performed using a volume chosen by the operator. 1000 liters is the reference volume for verifying that specifications have been respected (4 hours of battery life for 1000-liter samples).

If the volume generally used is, for example, 100 liters, then it is wiser to perform the test on 100 liter volumes.

 To check battery autonomy:

− Note the autonomy displayed when the instrument is turned on.

− Using Menu 1, select a 1000 liter sample volume.

− Go to Menu 6.

• Press the <START> button to run the battery discharge cycle.

 To select menu 6,

• Press <MENU> 6 times.

The display indicates:

 The battery discharge cycle can be stopped as follows:

• Press <STOP>.

 To resume at a later time,

• Press <START>.

CAUTION! If you wait more than 5 minutes, Air IDEAL 3P will turn off.

Air IDEAL® 3P User’s Manual 3-25

Using Air IDEAL 3P

Starting sampling

CAUTION! Before starting, check that Air IDEAL 3P is fitted with a sampling grid and not its

protective cover otherwise it could undergo irreversible damage which is not covered by the bioMérieux warranty.

 If the protective cover remains on Air IDEAL 3P, the following alarm message is displayed

• Remove the protection cover.

• Install the grid.

• Press <MENU> to return to the welcome message.

 To start sampling,

 Turn on Air IDEAL 3P,

• Press <ON/OFF>.

after several seconds and sampling stops automatically:

The display indicates:

• Press <START/STOP>.

 During sampling, the display indicates:

− The

− The last sample volume recorded is automatically displayed (shown as

SAMPLE VOL display flashes during the sampling phase and the volume counter

is displayed.

ZZZL).

3-26 Air IDEAL® 3P User’s Manual

Using Air IDEAL 3P

Starting sampling

A buzzer indicates the end of sampling (6 short buzzes) and the display returns to the original message with automatic correction of the remaining battery life.

 If a delayed start-up has been programmed, the display indicates:

TIMED flashes during the count-down phase.

Note: The motor stops 2 liters before the total volume has been sampled.

 Last sampling not terminated:

If Air IDEAL 3P was turned off during sampling, the following message appears the next time the instrument is turned on:

CAUTION! In this case, the user will take all necessary precautions to deal with this interrupted

sampling.

• Press <MENU> to return to the welcome message.

Air IDEAL® 3P User’s Manual 3-27

Using Air IDEAL 3P

Starting sampling

Stopping the motor

Note: It is always possible to stop a sampling operation under way by pressing <START/STOP>.

Note: If stoppage during sampling is more than 5 minutes, Air IDEAL 3P will turn off.

• Press <START/STOP>.

If the motor is stopped during the program, i.e. during the delayed start-up count-down phase or during the sampling phase, the value displayed freezes and the display continues to flash.

 To resume sampling,

• Press <START/STOP>.

If the motor is restarted after being stopped during a program, the program will resume from the point where it was stopped.

3-28 Air IDEAL® 3P User’s Manual

Setting Air IDEAL 3P parameters (summary diagram)

Using Air IDEAL 3P

4 x

1 x

Menu 1 : Selection of one of

the 4 sampling volumes

among the 4 pre-programmed

Press < + > or < - > until the desired volume in memory is

volumes.

displayed.

("Preset volume")

2 x

Menu 2 : Selection of a

sampling volume not in

memory.

Press < + > or < - > until the desired volume is displayed.

("CHOSEN VOLUME")

3 x

Menu 3 : Modification of the 4

pre-programmed sampling

volumes

"PROGRAM 1, 2, 3 et 4

Press < + > or < - > until the chosen volume is displayed.

Air IDEAL® 3P User’s Manual 3-29

Using Air IDEAL 3P

Setting Air IDEAL 3P parameters (summary diagram)

4 x

Menu 4 : Programming

delayed start-up.

5 x

Menu 5 : Programming

sequences samplings.

6 x

Menu 6 : Battery autonomy

test

Select delay before starting

Press < + > or < - > until the

delay value is displayed.

Press < + > or < - > until the desired number is displayed.

1 x

Press < + > or < - > until the

desired time is displayed.

Select number of sequences

Select time between sequences

3-30 Air IDEAL® 3P User’s Manual

4 Procedure

Principle

Implementation and following of a procedure to measure aerobiocontamination corresponds to a process based on prevention, which involves:

• Evaluating the current level and standard of hygiene and controls.

• Selecting the critical areas to be controlled.

• Establishing a reference level and an alert level for each of the critical points.

• Developing a sampling plan.

• Preparing a document for recording air sampling results.

• Preparing a plan for corrective action in case of deviation.

The result of sampling should provide information on the level of risk, global hygiene conditions and environment.

Procedure

The procedure must be adapted to the actual conditions in which air sampling will take place (contaminated areas, clean areas, sterile areas, etc.)

When strictly followed, the procedure guarantees good quality sampling and should include:

− Operators’ qualifications.

− Names of qualified operators.

− Hygiene of operators (clothes, hands...).

− Protocol for disinfection of the air sampler.

− Detailed steps of the procedure.

Air IDEAL® 3P User’s Manual 4-1

Procedure

How to obtain a good quality sample

Precautions of use

• Check the condition of the instrument and sampling grid.

• When unscrewing and screwing on the sampling grid avoid touching the perforated zone.

The choice of medium depends on the area to be controlled and the type of microorganism to be isolated.

The agar in 90 mm Petri dishes, must be at least 2.5 mm thick at the center and have a flat surface. It should not present dehydration or humidity droplets.

• During sampling, avoid any unnecessary movement, do not pass in front of the

instrument or cough etc.

• Begin by collecting from low contaminated areas.

• Collect several samples in each zone in order to obtain results that can be used for

statistical studies.

• Clean the instrument after use and sterilize sampling grids.

• Recharge the battery if necessary.

• Place the instrument in its carrying case and store it in a suitable place.

Sampling

• Turn on the instrument.

• Check the remaining battery life.

• Place the instrument on a flat surface (work bench, table...) in a vertical or horizontal

• Select the sample volume according to the critical area to be controlled.

• Remove the protective cover or the sampling grid.

• Place the Petri dish with its cover between the attachment clips and make sure it is

• Remove the Petri dish cover and place it on a clean surface.

• Screw on the sampling grid corresponding to the type of plate used.

• Perform sampling rapidly.

• The instrument indicates the end of sampling ( 6 buzzes).

• Unscrew the sampling grid.

• Carefully remove the Petri dish without touching the agar.

• Put the cover back on the Petri dish used.

• Put the protective cover back on.

• Mark the date, time and sample location on the Petri dish.

Incubation and reading

position, or set it up on its tripod.

correctly positioned.

The Petri dishes must be placed in the incubator as rapidly as possible.

After incubation, read as follows:

− Count the number of C.F.U (Colony Forming Units) which have grown and refer to the reading table for the final result (see page 7-1).

− If results are not acceptable, proceed with colony identification to orientate corrective action.

4-2 Air IDEAL® 3P User’s Manual

Sampling plan

Procedure

Sampling plan

The sampling plan must be drawn up very carefully and followed strictly. The aim is to guarantee that the values obtained are comparable. Any discrepancy between values should reveal a variation in aerobiocontamination.

A sampling plan must include:

− the critical points to be controlled

− the following must be mentioned for each point controlled:

• the time and frequency,

• the microorganism(s) to be isolated,

• the media used,

• the revivification conditions,

• the sample volume,

• the number of samples per area controlled,

• the reference level and the alert level,

In practice, the alert level can be fixed at 3 times the reference level.

• sampling conditions (temperature, hygrometry, staff numbers, activity...).

Recording and evaluating results

The person in charge of controlling the quality of air collects the results recorded.

− Results which give expected values are validated.

− If unacceptable results are obtained, corrective action may be necessary.

The document for recording results must include:

− date and time of sample,

− operator’s name,

− critical point controlled,

− the situation of the control in relation to the activity (production cycle, pre or post

operative, etc.),

− detailed stages of the procedure,

− level of internal activity (number of people present, number of lines running, etc.),

− thermohygrometric conditions (real or reference),

− microorganism to be isolated,

− culture medium used,

− incubation conditions (duration, temperature),

− date and time of incubation,

− date and time of reading,

− reference level and alert level,

− result: acceptable/unacceptable,

− decision: validation/ corrective action.

Air IDEAL® 3P User’s Manual 4-3

5 Maintenance

Preventive maintenance

CAUTION! No preventive maintenance needs to be performed by the user.

If an anomaly occurs on your instrument, call bioMérieux or your local distributor. Do not open the shell of the instrument.

Dispose of the battery in a suitable container.

Routine servicing

The routine servicing operations to be carried out by the user are:

− Battery recharging.

− Cleaning and disinfection.

− Sterilization of the sampling grid.

Cleaning and decontamination

All parts, including the case (inside and outside), can be cleaned with soapy water, rinsed and dried.

The stainless steel strip for attachment of Petri dishes can be dismantled for cleaning.

IMPORTANT! Do not use concentrated peracetic acid, acetone or chlorinated solvents (chloroform,

etc.) on the shell or keyboard.

Sterilization of grids

Air IDEAL 3P is delivered with a set of 5 grids. They can be sterilized by autoclaving at

134°C for 18 min. bioMérieux has validated that the grid specifications and performance are not altered after autoclaving up to:

- 40 times at 134°C for 18 minutes

- 200 times at 121°C for 20 minutes

After autoclaving 14 times or more, the grid may begin to turn yellow and may become difficult to screw onto the instrument.

IMPORTANT! Do not sterilize under a flame.

Decontamination of the external part

All the external parts of Air IDEAL 3P can be decontaminated with most usual disinfectants (70% isopropanol, hydrogen peroxide, 70% ethanol, quaternary ammonium).

Decontamination of the air circuit

bioMérieux has validated the bacteriological efficacy of air circuit decontamination with ClearKlens IPA

Decontamination of the air circuit

Decontamination of the instrument according to this protocol with ClearKlens IPA (Johnson Diversey) has been validated. A summary of this validation is available on request.

®

(Johnson Diversey).

 Decontamination protocol:

• Spray twice with 70% isopropyl alcohol with the motor off: one spray at the air inlet, the

other at the outlet.

• Allow to react for 15 minutes before using the instrument.

Air IDEAL® 3P User’s Manual 5-1

Maintenance

Routine servicing

Decontamination in a glove box

Air IDEAL 3P was specially developed for use in production or control glove boxes. The

suitability of Air IDEAL 3P for use in glove boxes was evaluated by the SKAN ag "Center of Competence for Isolator Technology".

 Two parameters were tested:

− The capacity of Air IDEAL 3P to withstand standard decontamination cycles in gloves boxes.

− The capacity of a standard decontamination cycle to disinfect the different types of materials that compose the Air IDEAL 3P.

A summary of this validation is available on request.

Airflow control

The airflow control must be performed once a year from the date of the last control or adjustment.

IMPORTANT! The airflow control must be done by a qualified person who has all the necessary

equipment to perform this task and to enter the data into the Air IDEAL 3P.

5-2 Air IDEAL® 3P User’s Manual

6 Troubleshooting

Error message/signal Possible cause Recovery procedure

REMOVE PROTECTION

COVER

ERROR

TIME > 5 H

MOTOR

JAMMED

The protection cover was not removed. Install a grid.

The total duration of sequences and intervals is too long.

Mechanical incident that blocks the motor.

Repeat programming.

Inspect the black screen under the Petri dish attachment strip. Press <MENU> to return to the welcome message.

Air IDEAL® 3P User’s Manual 6-1

7 Appendix

Using the reading table

The following reading tables indicate the most probable number of microorganisms collected per plate (MPN collected) with respect to the number of agglomerates of colonies counted on the agar (CFU read).

The MPN value is calculated from the CFU count, using FELLER’s law. This statistical correction corresponds to the random passing of bacteria through the orifices of the grid.

It quantifies for each “visited” orifice, (i.e. for each cluster counted) and as a function of the total number of clusters counted, the most probable number of bacteria which make up the cluster concerned, i.e. the number of bacteria having passed through the same orifice.

The application of the statistical correction assumes that the CFU count on the agar concerns the number of colony clusters, i.e. the number of orifices with a positive impact, without distinguishing, within a given cluster, the number of confluent colonies of which it is made up.

In order to determine the most probable number of microorganisms collected per cubic meter, the most probable number of microorganisms collected per plate (MPN

collected) must be multiplied by 1000 and divided by the volume sampled in liters.

Example: Volume of air sampled = 50 liters

CFU count: 120 MPN value: 159, 380905 Result expressed as the MPN collected per cubic meter = 159 x 1000 / 50 = 3188

Information on FELLER’s law

The formula for FELLER’s law is the following:

MPN = N .( 1/N + 1/N-1 + 1/N-2 + ………. + 1/N-CFU+1)

where:

MPN = most probable number of bacteria having passed through the orifices of the grid

N= number of orifices on the grid.

CFU = colony forming units, value obtained by the laboratory.

In the case of a sampling grid for which the passage of a particle through a given orifice of the grid is purely random, there is a probability that, during a sampling cycle, several particles pass through the same orifice and are therefore counted as a single and unique CFU, while other orifices are not passed through by any particles.

The closer the CFU count obtained by the laboratory is to N, (total number of orifices on the grid), the higher the probability is.

Moreover, the notion of probability density arises:

Example: for a given CFU value:

− there is a probability p2 that the same orifice is passed through by 2 particles

− there is a probability p3 that the same orifice is passed through by 3 particles

− there is a probability pi that the same orifice is passed through by i particles etc…

Air IDEAL® 3P User’s Manual 7-1

Appendix

Reading tables

IMPORTANT! In practice, and whenever possible, to minimize statistical correction, it is

Reading tables

The probability pi decreases as i increases.

It is therefore particularly relevant to apply the statistical correction using FELLER’s law when high CFU values are read, i.e. close to N, i.e. in the case of agar plates almost completely saturated with colonies.

recommended to adjust the sample volume so that the CFU count does not exceed

100.

CFU

count

1 1 37 39,770782 73 85,202311 109 140,067796

2 2,003788 38 40,933063 74 86,582519 110 141,766514

3 3,011392 39 42,100464 75 87,969954 111 143,476191

4 4,022843 40 43,273030 76 89,364690 112 145,196971

5 5,038168 41 44,450808 77 90,766807 113 146,928997

6 6,057399 42 45,633844 78 92,176381 114 148,672418

7 7,080565 43 46,822184 79 93,593494 115 150,427385

8 8,107697 44 48,015878 80 95,018225 116 152,194051

9 9,138825 45 49,214973 81 96,450657 117 153,972575

10 10,173982 46 50,419519 82 97,890875 118 155,763115

11 11,213197 47 51,629564 83 99,338962 119 157,565836

12 12,256504 48 52,845161 84 100,795006 120 159,380905

13 13,303935 49 54,066359 85 102,259094 121 161,208491

14 14,355523 50 55,293211 86 103,731317 122 163,048769

15 15,411300 51 56,525769 87 105,211764 123 164,901916

16 16,471300 52 57,764087 88 106,700528 124 166,768113

17 17,535557 53 59,008218 89 108,197703 125 168,647546

18 18,604105 54 60,258218 90 109,703385 126 170,540403

19 19,676979 55 61,514142 91 111,217670 127 172,446878

20 20,754215 56 62,776047 92 112,740659 128 174,367167

21 21,835848 57 64,043990 93 114,272451 129 176,301474

22 22,921913 58 65,318028 94 115,813148 130 178,250003

23 24,012448 59 66,598221 95 117,362856 131 180,212966

24 25,107490 60 67,884629 96 118,921679 132 182,190578

25 26,207075 61 69,177312 97 120,489727 133 184,183059

26 27,311241 62 70,476332 98 122,067108 134 186,190635

27 28,420028 63 71,781750 99 123,653934 135 188,213536

28 29,533473 64 73,093631 100 125,250320 136 190,251998

29 30,651617 65 74,412039 101 126,856380 137 192,306261

30 31,774498 66 75,737039 102 128,472234 138 194,376574

31 32,902158 67 77,068698 103 130,098001 139 196,463188

32 34,034636 68 78,407081 104 131,733803 140 198,566362

33 35,171976 69 79,752259 105 133,379766 141 200,686362

34 36,314217 70 81,104300 106 135,036016 142 202,823459

35 37,461403 71 82,463274 107 136,702683 143 204,977931

36 38,613577 72 83,829254 108 138,379898 144 207,150062

Corrected

MPN

CFU

count

Corrected

MPN

CFU

count

Corrected

MPN

CFU

count

Corrected

MPN

7-2 Air IDEAL® 3P User’s Manual

Appendix

Reading tables

CFU

count

145 209,340144 176 288,153475 207 400,832773 238 600,854201

146 211,548478 177 291,131003 208 405,401739 239 610,669016

147 213,775369 178 294,142367 209 410,050862 240 620,861324

148 216,021131 179 297,188344 210 414,783004 241 631,461324

149 218,286089 180 300,269739 211 419,601186 242 642,502990

150 220,570571 181 303,387386 212 424,508594 243 654,024729

151 222,874919 182 306,542148 213 429,508594 244 666,070184

152 225,199481 183 309,734919 214 434,604748 245 678,689231

153 227,544613 184 312,966627 215 439,800826 246 691,939231

154 229,910685 185 316,238232 216 445,100826 247 705,886600

155 232,298072 186 319,550732 217 450,508989 248 720,608822

156 234,707163 187 322,905162 218 456,029823 249 736,197057

157 237,138356 188 326,302598 219 461,668120 250 752,759557

158 239,592059 189 329,744156 220 467,428990 251 770,426224

159 242,068695 190 333,230999 221 473,317879 252 789,354796

160 244,568695 191 336,764332 222 479,340606 253 809,739411

161 247,092504 192 340,345413 223 485,503397 254 831,822744

162 249,640581 193 343,975550 224 491,812921 255 855,913653

163 252,213397 194 347,656105 225 498,276335 256 882,413653

164 254,811436 195 351,388500 226 504,901335 257 911,858098

165 257,435198 196 355,174214 227 511,696207 258 944,983098

166 260,085198 197 359,014794 228 518,669891 259 982,840241

167 262,761966 198 362,911853 229 525,832053 260 1027,006907

168 265,466048 199 366,867077 230 533,193165 261 1080,006907

169 268,198007 200 370,882228 231 540,764593 262 1146,256907

170 270,958423 201 374,959151 232 548,558711 263 1234,590241

171 273,747897 202 379,099776 233 556,589014 264 1367,090241

172 276,567046 203 383,306125 234 564,870264 265 1632,090241

173 279,416508 204 387,580319 235 573,418651

174 282,296943 205 391,924581 236 582,251984

175 285,209031 206 396,341248 237 591,389915

Corrected

MPN

CFU

count

Corrected

MPN

CFU

count

Corrected

MPN

CFU

count

Corrected

MPN

Air IDEAL® 3P User’s Manual 7-3

Notes

..............................................................................................................................................................................................................................................................................................

Air IDEAL® 3P User’s Manual Notes-1

..............................................................................................................................................................................................................................................................................................

Notes-2 Air IDEAL® 3P User’s Manual

bioMerieux Air IDEAL 3P User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Revisions

Table of contents

List of figures

1 How to use this manual

Finding topics and procedures

Glossary

Typographic conventions

The Air IDEAL 3P keypad

2 Functional description

Presentation

3 Using Air IDEAL 3P

Description

General specifications

Unpacking Air IDEAL 3P

Recommendations for installation and use

Sampling positions

Screwing on the sampling grid

Electrical power supply

Programming

Turning on Air IDEAL 3P

Turning off Air IDEAL 3P

Starting sampling

Setting Air IDEAL 3P parameters (summary diagram)

4 Procedure

Principle

Procedure

How to obtain a good quality sample

Sampling plan

Recording and evaluating results

5 Maintenance

Preventive maintenance

Routine servicing

6 Troubleshooting

7 Appendix

Using the reading table

Information on FELLER’s law

Reading tables

Notes