3B SCIENTIFIC® PHYSICS
Monocular Course Microscope Model 300 LED (115 V) 1013366
Monocular Course Microscope Model 300 LED (230 V) 1013127
Instruction Manual
07/13 ALF
1 Eyepiece
2 Tube
3 Revolver with objectives
4 Object stage
5 Condensor with iris
diaphragm and filter holder
6 Lamp housing
7 Illumination control
8 Mains switch
9 Adjustment knob for
mechanical stage
10 Adjustment knob for fine
and coarse focusing
11 Object guide
12 Lock screw for object stage
13 Head lock screw
14 Stand
1. Safety notes
• For power supply use only electrical sockets
with ground contact.
2. Description, technical data
The monocular course microscope allows twodimensional viewing of objects (thin sections of plant
or animal specimen) in 40x to 1000x magnification.
The microscope 1013366 is for operation with a
mains voltage of 115 V (±10%), and the
1013127 unit is for operation with 230 V (±10%).
Stand: Robust, all metal stand with arm permanently connect to the base. Focussing by means
of separate knobs for coarse and fine adjustment located on either side of the stand and
operated by rack and pinion drive with ball bearings, adjustable stopper for protecting the object
slides and objective
Tube: Monocular inclined 45°, head rotation 360°
Eyepiece: Widefield eyepiece WF 10x 18 mm
Objectives: Revolver with 4 DIN achromatic
objectives 4x / 0.10, 10x / 0.25, 40x / 0.65, 100x
/ 1.25 oil (with specimen protection)
Magnification: 40x, 100x, 400x, 1000x
Object stage: x-y cross table, 125 x 130 mm
with object guide and coaxial adjustment knobs
perpendicular to the object stage, adjustment
range 70 x 30 mm
2
Illumination: Adjustable LED lighting integrated
in base
Power supply: Via plug-in power supply 6 V
DC, 200 mA
Mains connection: 1013366: 115 V, 50/60 Hz;
1013127: 230 V, 50/60 Hz
Condenser: Abbe condenser N.A.1.25 NA 0.65
with iris diaphragm , filter holder and blue filter,
1
2
,
focussed via rack and pinion drive
Dimensions: 220 x 154 x 359 mm³ approx.
Weight: 4.5 kg approx.
3. Unpacking and assembly
The microscope is packed in a molded styrofoam container.
• Take the container out of the carton remove
the tape and carefully lift the top half off the
container. Be careful not to let the optical
items (objectives and eyepieces) drop down.
• To avoid condensation on the optical compo-
nents, leave the microscope in the original packing to allow it to adjust to room temperature.
• Using both hands (one around the pillar and
one around the base), lift the microscope from
the container and put it on a stable desk.
• The objectives will be found within individual
protective vials. Install the objectives into the
microscope nosepiece from the lowest
magnification to the highest, in a clockwise
direction from the rear.
• Put the head onto the top of the stand and
tighten the head-lock-screw. Insert the eyepiece into the tube.
4. Operation
• Set the microscope on a level table.
• Place the object to be observed in the center
of the object plate. Use the clips to fasten it
into place. Make certain that the specimen is
centered over the opening in the stage.
• Connect the mains cable to the net and turn
on the switch to get the object illuminated.
• Make certain that the specimen is centered
over the opening in the stage.
• To obtain a high contrast, adjust the back-
ground illumination by means of the iris diaphragm and the variable illumination control.
• Rotate the nosepiece until the objective with
the lowest magnification is pointed at the
specimen. There is a definite “click” when
each objective is lined up properly.
NOTE: It is best to begin with the lowest power
objective. This is important to reveal general
structural details with the largest field of view
first. Than you may increase the magnification
as needed to reveal small details. To determine
the magnification at which you are viewing a
specimen, multiply the power of the eyepiece by
the power of the objective.
• Adjust the coarse-focusing-knob which
moves the stage up until the specimen is focused. Be careful that the objective does not
make contact with the slide at any time. This
may cause damage to the objective and/or
crack your slide.
• Adjust the fine-focusing-knob to get the im-
age more sharp and more clear.
• Colour filters may be inserted into the filter
holder for definition of specimen parts. Swing
the filter holder out and insert colour filters.
• Use the knobs of the mechanical stage to
move the slide side-, back- and forwards.
The vernier provides acc urate loc ation of the
specimen area.
• Always turn off the light immediately after
use.
• Be careful not to spill any liquids on the mi-
croscope.
• Do not mishandle or impose unnecessary
force on the microscope.
• Do not wipe the optics with your hands.
• Do not attempt to service the microscope
yourself.
5. Storage, cleaning, disposal
• Keep the microscope in a clean, dry and
dust free place.
• When not in use always cover the micro-
scope with the dust cover.
• Do not expose it to temperatures below 0°C
and above 40°C and a max. relative humidity of over 85%.
• Always unplug the mains plug before clean-
ing or maintenance.
• Do not clean the unit with volatile solvents or
abrasive cleaners.
• Do not disassemble objective or eyepieces
to attempt to clean them.
• Use a soft linen cloth and some ethanol to
clean the microscope.
• Use a soft lens tissue to clean the optics.
• The packaging should be disposed of at
local recycling points.
• Should you need to
dispose of the equipment itself, never throw
it away in normal domestic waste. Local
regulations for the disposal of electrical
equipment will apply.
3B Scientific GmbH • Rudorffweg 8 • 21031 Hamburg • Germany • www.3bscientific.com
Subject to technical amendments
© Copyright 2013 3B Scientific GmbH