3B Scientific Monocular Course Microscope 300 User Manual

3B SCIENTIFIC® PHYSICS

Monocular Course Microscope Model 300 (115 V) 1003270
Monocular Course Microscope Model 300 (230 V) 1003271

Instruction Manual

03/13 ALF

1 Eyepiece
2 Tube
3 Revolver with objectives
4 Object stage
5 Condensor with iris

diaphragm and filter holder
6 Lamp housing
7 Mains switch
8 Adjustment knob for

mechanical stage
9 Adjustment knob for fine

and coarse focusing
10 Object guide
11 Lock screw for object stage
12 Head lock screw
13 Stand

1. Safety notes

• For power supply use only electrical sockets

with ground contact.

2. Description, technical data

The monocular course microscope allows two­dimensional viewing of objects (thin sections of plant
or animal specimen) in 40x to 1000x magnification.

The microscope 1003270 is for operation with a
mains voltage of 115 V (±10%), and the
1003271 unit is for operation with 230 V (±10%).

Stand: Robust, all metal stand with arm perma­nently connect to the base. Focussing by means
of separate knobs for coarse and fine adjust­ment located on either side of the stand and
operated by rack and pinion drive with ball bear­ings, adjustable stopper for protecting the object
slides and objective

Tube: Monocular inclined 45°, head rotation 360°

Eyepiece: Widefield eyepiece WF 10x 18 mm
Objectives: Revolver with 4 DIN achromatic

objectives 4x / 0.10, 10x / 0.25, 40x / 0.65, 100x
/ 1.25 oil (with specimen protection)

Magnification: 40x, 100x, 400x, 1000x
Object stage: x-y cross table, 125 x 130 mm

with object guide and coaxial adjustment knobs
perpendicular to the object stage, adjustment
range 70 x 30 mm

Illumination: 5 W fluorescent lamp incorporated
in the base integrated in base, with a converging
lens in lamp shaft, power supply 115 V resp.
230 V 50/60 Hz

Condenser: Abbe condenser N.A.1.25 NA 0.65
with iris diaphragm , filter holder and blue filter,
focussed via rack and pinion drive

Dimensions: 220 x 154 x 359 mm³ approx.
Weight: 4.5 kg approx.

2

2

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1

3. Unpacking and assembly

The microscope is packed in a molded styro­foam container.

• Take the container out of the carton remove

the tape and carefully lift the top half off the
container. Be careful not to let the optical
items (objectives and eyepieces) drop down.

• To avoid condensation on the optical compo-

nents, leave the microscope in the original pack­ing to allow it to adjust to room temperature.

• Using both hands (one around the pillar and

one around the base), lift the microscope from
the container and put it on a stable desk.

• The objectives will be found within individual

protective vials. Install the objectives into the
microscope nosepiece from the lowest
magnification to the highest, in a clockwise
direction from the rear.

• Put the head onto the top of the stand and

tighten the head-lock-screw. Insert the eye­piece into the tube.

4. Operation

4.1 General information

• Set the microscope on a level table.

• Place the object to be observed in the center

of the object plate. Use the clips to fasten it
into place. Make certain that the specimen is
centered over the opening in the stage.

• Connect the mains cable to the net and turn

on the switch to get the object illuminated.

• Make certain that the specimen is centered

over the opening in the stage.

• Adjust the aperture of the iris diaphragm to

get the background brightness suitable for a
high contrast image.

• Rotate the nosepiece until the objective with

the lowest magnification is pointed at the
specimen. There is a definite “click” when
each objective is lined up properly.

NOTE: It is best to begin with the lowest power
objective. This is important to reveal general
structural details with the largest field of view
first. Than you may increase the magnification
as needed to reveal small details. To determine
the magnification at which you are viewing a
specimen, multiply the power of the eyepiece by
the power of the objective.

• Adjust the coarse-focusing-knob which

moves the stage up until the specimen is fo­cused. Be careful that the objective does not
make contact with the slide at any time. This
may cause damage to the objective and/or
crack your slide.

• Adjust the fine-focusing-knob to get the im-

age more sharp and more clear.

• Colour filters may be inserted into the filter

holder for definition of specimen parts. Swing
the filter holder out and insert colour filters.

• Use the knobs of the mechanical stage to

move the slide side-, back- and forwards.
The vernier provides acc urate loc ation of the
specimen area.

• Always turn off the light immediately after use.

• Be careful not to spill any liquids on the mi-

croscope.

• Do not mishandle or impose unnecessary

force on the microscope.

• Do not wipe the optics with your hands.

• Do not attempt to service the microscope

yourself.

4.2 Changing the fuse

• Turn off the power switch and unplug the

mains plug.

• Unscrew the fuse holder on the back of the

stand base with a screwdriver.

• Replace the fuse and reinsert the holder in

its socket.

5. Storage, cleaning, disposal

• Keep the microscope in a clean, dry and

dust free place.

• When not in use always cover the micro-

scope with the dust cover.

• Do not expose it to temperatures below 0°C

and above 40°C and a max. relative humid­ity of over 85%.

• Always unplug the mains plug before clean-

ing or maintenance.

• Do not clean the unit with volatile solvents or

abrasive cleaners.

• Do not disassemble objective or eyepieces

to attempt to clean them.

• Use a soft linen cloth and some ethanol to

clean the microscope.

• Use a soft lens tissue to clean the optics.

• The packaging should be disposed of at

local recycling points.

• Should you need to

dispose of the equip­ment itself, never throw
it away in normal do­mestic waste. Local
regulations for the dis­posal of electrical
equipment will apply.

3B Scientific GmbH • Rudorffweg 8 • 21031 Hamburg • Germany • www.3bscientific.com

Subject to technical amendments

© Copyright 2013 3B Scientific GmbH