YSI 600DW-B User Manual

6-SERIES
DRINKING

WATER
SYSTEMS

600DW-B Sonde
6920DW Sonde
650 MDS Display/Logger
6500 Process Monitor

Environmental
Monitoring
Systems

SAFETY NOTES

TECHNICAL SUPPORT AND WARRANTY INFORMATION

Contact information for technical support and warranty information on YSI’s Environmental
Monitoring Systems products can be found in Section 8, Warranty and Service Information.

COMPLIANCE

When using the YSI 6-Series sondes in a European Community (CE) country, please be aware
that electromagnetic compatibility (EMC) performance issues may occur under certain
conditions, such as when the sonde is exposed to certain radio frequency fields.

If you are concerned with these issues, consult the Declaration of Conformity that was enclosed
with your instrument. Specific conditions where temporary sensor problems may occur are listed
in this document.

If you are unable to locate the Declaration of Conformity that was shipped with your instrument,
contact your local YSI representative, or YSI Customer Service in Yellow Springs, Ohio for a
copy of the document. See Section 8, Warranty and Service Information for contact
information.

SPECIFICATIONS

For general specifications for all YSI Environmental Monitoring Systems products included in
this manual, please see Appendix M, Specifications.

GENERAL SAFETY CONSIDERATIONS

For Health and Safety issues concerning the use of the calibration solutions with the sondes,
please see Appendix A, Health and Safety.

NOTICE

Information contained in this manual is subject to change without notice. Effort has been made to
make the information contained in this manual complete, accurate, and current. YSI shall not be
held responsible for errors or omissions in this operations manual.

WARNING:

When caring for your sonde, remember that the sonde is sealed at the factory, and there is never a
need to gain access to the interior circuitry of the sonde. In fact, if you attempt to disassemble the
sonde, you would void the manufacturer's warranty.

TABLE OF CONTENTS

SECTION 1 INTRODUCTION

1.1 ABOUT YSI 1-1

1.2 HOW TO USE THIS MANUAL 1-1

1.3 UNPACKING AND INSPECTION 1-2

SECTION 2 6920DW AND 600DW-B SONDES

2.1 GETTING STARTED 2-1

2.2 CONNECTING YOUR SONDE 2-2

2.3 PREPARING THE SONDE FOR USE 2-5

2.4 ECOWATCH FOR WINDOWS – GETTING STARTED 2-18

2.5 SONDE SOFTWARE SETUP 2-18

2.6 GETTING READY TO CALIBRATE 2-26

2.7 TAKING READINGS 2-39

2.8 USING ECOWATCH TO UPLOAD AND ANALYZE DATA 2-45

2.9 SONDE MENU 2-59

2.10 CARE, MAINTENANCE, AND STORAGE 2-95

SECTION 3 650 MDS DATA LOGGER

3.1 INTRODUCTION 3-1

3.2 GETTING STARTED 3-1

3.3 SETTING UP THE 650 3-18

3.4 SONDE MENU INTERFACE 3-21

3.5 LOGGING DATA WITH THE 650 3-27

3.6 MANAGING 650 FILES 3-46

3.7 UPLOADING DATA FROM SONDES 3-51

3.8 USING GPS WITH 650 3-51

3.9 USING THE 650 BAROMETER 3-53

3.10 UPGRADING 650 SOFTWARE 3-55

3.11 TROUBLESHOOTING 3-56

3.12 FERRITE BEAD INSTALLATION 3-57

3.13 SAFETY CONSIDERATIONS 3-58

3.14 650 MDS SPECIFICATIONS 3-61

SECTION 4 ECOWATCH FOR WINDOWS

4.1 INTRODUCTION 4-1

4.2 DATA ACQUISITION AND ANALYSIS 4-7

4.3 ECOWATCH MENU 4-12

SECTION 5 PRINCIPLES OF OPERATION

5.1 CONDUCTIVITY 5-1

5.2 SALINITY 5-2

5.3 TOTAL DISSOLVED SOLIDS (TDS) 5-2

5.4 OXIDATION REDUCTION POTENTIAL (ORP) 5-3

5.5 pH 5-4

5.6 TEMPERATURE 5-5

5.7 FREE CHLORINE 5-5

5.8 NITRATE 5-9

5.9 AMMONIUM AND AMMONIA 5-10

5.10 CHLORIDE 5-12

5.11 TURBIDITY 5-14

5.12 CHLOROPHYLL 5-16

5.13 RHODAMINE WT 5-23

SECTION 6 TROUBLE SHOOTING

6.1 CALIBRATION ERRORS 6-1

6.2 SONDE COMMUNICATION PROBLEMS 6-2

6.3 SENSOR PERFORMANCE PROBLEMS 6-3

SECTION 7 COMMUNICATION

7.1 OVERVIEW 7-1

7.2 HARDWARE INTERFACE 7-1

7.3 RS-232 INTERFACE 7-2

7.4 SDI-12 INTERFACE 7.2

SECTION 8 WARRANTY AND SERVICE INFORMATION

8.1 LIMITATIONS OF WARRANTY 8-1

8.2 AUTHORIZED SERVICE CENTERS 8-2

8.3 CLEANING INSTRUCTIONS 8-2

APPENDIX A HEALTH AND SAFETY A-1

APPENDIX B REQUIRED NOTICE B-1

APPENDIX C ACCESSORIES AND CALIBRATION STANDARDS C-1

APPENDIX D EMC PERFORMANCE D-1

APPENDIX E SPECIFICATIONS E-1

APPENDIX F QUICK START GUIDE TO DRINKING WATER APPLICATIONS F-1

APPENDIX G USING YOUR DW SONDE WITH A YSI 6500 PROCESS MONITOR G-1

APPENDIX H FREQUENTLY ASKED QUESTION ABOUT DW SYSTEMS H-1

Introduction Section 1

SECTION 1 INTRODUCTION

1.1 ABOUT YSI INCORPORATED

From a three-man partnership in the basement of the Antioch College science building in 1948, YSI has
grown into a commercial enterprise designing and manufacturing precision measurement sensors and
control instruments for users around the world. Although our range of products is broad, we focus on four
major markets: water testing and monitoring, health care, bioprocessing, and OEM temperature
measurement.

In the 1950s, Hardy Trolander and David Case made the first practical electronic thermometer using a
thermistor. This equipment was developed to supply Dr. Leland Clark with a highly sensitive and precise
temperature sensor for the original heart-lung machine. The collaboration with Dr. Clark has been critical
to the success of the company. In the 1960s, YSI refined a Clark invention, the membrane covered
polarographic electrode, and commercialized oxygen sensors and meters which revolutionized the way
dissolved oxygen was measured in wastewater treatment plants and environmental water. Today,
geologists, biologists, environmental enforcement personnel, officials of water utilities and fish farmers
recognize us as the leader in dissolved oxygen measurement.

In the 1970s, YSI again worked with Clark to commercialize one of his many inventions, the enzyme
membrane. This development resulted in the first practical use of a biosensor, in the form of a membrane
based on immobilized glucose oxidase, to measure blood sugar accurately and rapidly. In the next few
years, this technology was extended to other enzymes, including lactate oxidase for applications in
biotechnology, health care, and sports medicine.

In the early 1990s, YSI launched a line of multi-parameter water monitoring systems to address the
emerging need to measure non-point source pollution. Today we have thousands of instruments in the field
that operate with the push of a button, store data in memory, and communicate with computers. These
instruments (described in this manual) are ideal for profiling and monitoring water conditions in industrial
and wastewater effluents, lakes, rivers, wetlands, estuaries, coastal waters, and monitoring wells. If the
instrument has ‘on board’ battery power, it can be left unattended for weeks at a time with measurement
parameters sampled at your setup interval and data securely saved in the unit's internal memory. The fast
response of YSI’s sensors make the systems ideal for vertical profiling, and the small size of some our
sondes allows them to fit down 2-inch diameter monitoring wells. All of YSI’s multi-parameter systems for
surface and groundwater feature the YSI-patente d Rapid Pu l s e D i s s o l v e d O xygen Se nsor, whi ch exhib its
low-stirring dependence and provides accurate results without an expensive, bulky, and power-intensive
stirrer.

YSI has established a worldwide network of sel l i ng partners in 54 countries that includes laboratory sup pl y
dealers, manufacturers' representatives, and YSI’s sales force. A subsidiary, YSI UK, distributes products
in the United Kingdom, a sales office in Hong Kong supports YSI’s distribution part ners in Asia Pacific,
and YSI Japan supports distribution pa rt ners in Japan.

Through an employee stock ownership plan (ESOP), every employee is one of the owners. In 1994, the
ESOP Association named YSI the ESOP Company of the Year. YSI is proud of its products and are
committed to meeting or exceeding customers' expectations.

1.2 HOW TO USE THIS MANUAL

The manual is organized to let you quickly understand and operate the YSI 6-Series drinking water (DW)
systems. However, it cannot be stressed too strongly that informed and safe operation is more than just

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 1-1

Introduction Section 1

knowing which buttons to push. An unde rstanding of the principles of operation, calibration techniques,
and system setup is necessary to obtain accurate and meaningful results.

Because of the many features, configurations and applications of these versatile products, some sections of
this manual may not apply to the specific system you have purchased.

If you have any questions about this product or its application, please contact YSI’s customer service
department or authorized dealer for assistance. See Section 8, Warranty and Service Information for
contact information.

1.3 UNPACKING AND INSPECTION

Inspect the outside of the shipping box for damage. If any damage is detected, contact your shipping carrier
immediately. Remove the equipment from the shipping box. Some parts or supplies are loose in the
shipping box so check the packing material carefully. Check off all of the items on the packing list and
inspect all of the assemblies and components for damage.

If any parts are damaged or missing, contact your YSI representative immediately. If you purchased the
equipment directly from YSI, or if you do not know from which YSI representative your equipment was
purchased, refer to Section 8, Warranty and Service Information for contact information.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 1-2

Sondes Section 2

SECTION 2 6920DW AND 600DW-B SONDES

2.1 GETTING STARTED

The 6920DW and 600DW-B-B Drinking Water Monitoring Systems are multi-parameter, water quality
measurement, and data collection systems. Their
using a flow cell and a free chlorine sensor is provided with each instrument since this parameter is very
important in assessing the quality of drinking water. However, these instruments can also be used in a
variety of research, assessment, and regulatory compliance applications without a flow cell as long as the
user realizes that the performance of the free chlorine sensor will be significantly compromised under these
conditions. The primary difference between the “DW” instruments and those provided by YSI for surface
and ground water applications is that the oxygen sensor for the standard sondes has been replaced by a free
chlorine sensor in the 6920DW and 600DW-B. The free chlorine sensor also requires a different circuit
board which is resident in the instrument and this means that “DW” sondes are not capable of dissolved
oxygen measurement and, vice versa, standard sondes are not capable of free chlorine measurement even
though the probes themselves will fit in either of the instruments. Section 2 concentrates on how to set up
operate the “DW” sondes. For the purpose of terminology in this manual it should be realized that a sonde
is a torpedo-shaped water quality monitoring device that is placed in the water to gather water quality data.
Sondes may have multiple probes. Each probe may have one or more sensors that read water quality data.

The following list contains parameters that your sonde may measure. See Appendix E Specifications for
the specific parameters of each sonde.

• Free Chlorine

• Conductivity

• Specific Conductance

• Salinity

• Total Dissolved Solids

• Resistivity

• Temperature

• pH

• ORP

• Turbidity

• Nitrate-N

• Ammonia-N

• Ammonium-N

• Chloride

• Chlorophyll

• Rhodamine WT

This section is designed to quickly familiarize you with the hardware and software components of the
“DW” sondes and their accessories. You will then proceed to probe installations, cable connections,
software installation and finally basic communication with your Sonde. Diagrams, menu flow charts and
basic written instructions will guide you through basic hardware and software setup.

primary application is the monitoring of drinking water

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-1

Sondes Section 2

θ

θ

θ

θ

θ

2.2 CONNECTING YOUR SONDE

There are a number of ways in which you may connect the sondes to various computers, data collection
devices and VT-100 terminal emulators. To utilize the configuration that will work best for your
application, make sure that you have all of the components that are necessary. The following list and
diagrams provide a few possible configurations.

 Sonde to Lab Computer (recommended for initial setup)
 Sonde to Data Collection Platform
 Sonde to Portable Computer
 Sonde to YSI 650 MDS Display/Logger

Sonde to Lab Computer

DB-9

Power Supply*
6651: 220 VAC
6038: 110 VAC

MS-8

6095B

Adapter

Field Cable

You will need...

+

+

-

-

YSI 6920

YSI 6920

Sonde

Not required if you us e

*

sonde battery power.

Sonde
Field Cable
Computer with Com Port
6095B MS-8/DB-9 Adapter
Power Supply *

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-2

Sondes Section 2

Y

Y

Sonde to Data Collection Platform

6096 MS-8 Adapter with Flying Leads

MS-8

Field Cable

+

+

--

SI

692

Sonde

0

Sonde to Portable Computer

DB-9

DCP

You will need...

T

Sonde

T

Field Cable

T

6096 Adapter with leads

T

Data Collection Platform

MS-8

Field Cable

Sonde

6095B

Adapter

++

--

SI
692
0

You will need...

T

Sonde

T

Field Cable

T

Computer with Com Port

T

6095B MS-8/DB-9 Adapter

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-3

Sondes Section 2

Y

Y

r

Sonde to 650 Display/Logger

Environ

mental

Monitor

ing

YS

System

s

I

610-DM

650 MDS

MS-8

Field Cable

Sonde

Sonde to 6500 Process Monito

You will need...

T

Sonde

+

+

-

-

S

S

I

I

69

69

20

20

YSI 650 operates on C-cells or rechargeable batteries.

Sonde to 650 Display/Logger

T

Field Cable

T

650 MDS Display/Logger

You will need...

Sonde

-

-

Field Cable
650 MDS Display/Logger

YSI 6500 operates on 90-230 V AC Power.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-4

Sondes Section 2

D

2.3 PREPARING THE SONDE FOR USE

To prepare the sonde for calibration and operation, you need to install probes (sensors) into the connectors
on the sonde bulkhead. In addition to probe installation, you need to install a new membrane on the YSI
6572 Free Chlorine Probe. It is highly recommended that you install the free chlorine membrane before
installing the probe onto the bulkhead. The next step is providing power for the sondes, through batteries or
AC, and then connecting a cable. The four steps necessary for getting your sonde ready for use are listed
below.

Step 1 Installing the Free Chlorine Membrane – Section 2.3.1
Step 2 Installing the Probes – Section 2.3.2
Step 3 Supplyi n g Po w e r – Section 2.3.3
Step 4 Connecting a Cable – Section 2.3.4

2.3.1 STEP 1 - INSTALLING THE FREE CHLORINE PROBE MEMBRANE

The 6572 Free Chlorine probe is shipped with a protective cap on the sens o r tip w hich must be removed
before proceeding . Handle the probe with care. It is very important not to drop the probe and/or gouge the
probe tip. See Section 2.10.2, Probe Care and Maintenance, for information on how often the membrane
should be replaced.

Unpack the YSI 6572 Free Chlorine Probe Kit and follow the instructions below

FREE CHLORINE SENSOR ELECTROLYTE PREPARATION

Open the 6573 membrane kit which was shipped with your instrument and prepare the electrolyte solution.
Dissolve the KCl in the dropper bottle by filling it to the neck with deionized or distilled water and shak ing
until the solids are fully dissolved. After the KCl is dissolved, wait a few minutes until the solution is free
of bubbles.

1.

FREE CHLORINE SENSOR MEMBRANE INSTALLATION

Remove the protective Teflon membrane and O-ring from the sensor end of the YSI 6572 Free Chlorine
probe. CAUTION: The Teflon membrane MUST be removed for proper sensor function.

Remove the protective cap is installed on the connector end of the probe.

ADD DI OR DISTILLE

WATER

2.

PROT ECT IVE CA P

.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-5

Sondes Section 2

Locate the 6035 Reconditioning Kit which was supplied with your 6572 Free Chlorine Probe. Use one of
circular sanding disks the 6035 Reconditioning Kit to sand the face of the 6572 Probe according to the
following instructions.

• Hold the probe in a vertical position, place a sanding disk under your thumb, and stroke the probe

face in a direction parallel to the platinum electrode (located between
the two silver electrodes). The motion is similar to that used in
striking a match. Usually 10-15 strokes of the sanding disk are
sufficient to remove black deposits on the silver electrodes.
However, in extreme cases, more sanding may be required to
regenerate the original silver surface.

• Rinse the probe tip well with purified water to remove dust from

sanding.

CAUTION: You must sand your free chlorine sensor as described
above prior to installing a membrane to insure the surface tension
for probe surface/membrane interface.

Locate the YSI 6574 O-ring Installation Kit which was shipped with your sonde. Note that the kit contains
two items – a circular plate with a threaded hole and a cylindrical tool with a screwdriver-style handle as
shown in the picture below.

Thread the nut on the end of the 6572 probe into the base plate as shown in the picture below.

Place an o-ring from the 6573 membrane kit in the groove at the bottom of the tool as shown in the picture
below left.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-6

Sondes Section 2

Apply a few drops of KCl solution to the tip of the probe as shown in the picture above right. The fluid
should completely fill the small moat around the electrodes and form a meniscus on the tip of the sensor.

Remove a
below.

CAUTION: The blue sheets are simply protective spacers and CANNOT be used as
membranes for the free chlorine sensor.

As shown in the pictures below, lay the membrane on the probe tip that contains the electrolyte so that the
probe tip is approximately in the middle of the membrane.

O-Ring in Groove

white membrane sheet from the package in the 6573 membrane kit as shown in the picture

White Membrane

Meniscus

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-7

Sondes Section 2

As shown in the picture below, place the bottom of the o-ring installation tool over membrane/probe fa ce
and press down securely.

As shown in the picture below left, roll the o-ring off of the end of the installation tool so that it resides in
the o-ring groove of the probe. Be sure to hold onto the O-ring prior to removing the installation tool so
that it does not pop out of the groove. Then, as shown below right, remove the installation tool and make
certain that the o-ring is centered in the groove on probe end.

As shown in the picture below, use a razor blade or knife to trim away the excess membrane material.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-8

Sondes Section 2

The pictures below show a properly installed free chlorine membrane. Note that, because the membrane
will not stretch, there may be some points where the membrane is slightly “bunched up” in the o-ring
groove. This effect is normal and will not affect sensor function. Also note that the presence of bubbles
under the membrane is also normal and should not affect probe function

`

After installing the membrane, either proceed
above in the Quick Start Guide or store the probe in a beaker of tap water (NOT deioinized or distilled
water) as shown in the picture below to prevent drying out. Be sure to place the probe GENTLY into the
beaker to avoid damaging the membrane integrity.

immediately to the sensor activation protocol described

Note the presence of a bubble
after installation. This is
acceptable for a free chlorine
membrane.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-9

Sondes Section 2

P

NOTE CAREFULLY: Users who are accustomed to installing a Teflon membrane on a YSI dissolved
oxygen sensor should note that the methods recommended for the Teflon installation are not likely to work
well for
oxygen membrane installation techniques which are outline in the manuals for various YSI instruments,
including standard 6-series sondes utilizing the 6562 Rapid Pulse DO sensor, are NOT RECOMMENDED
for the free chlorine membrane installation.

the free chlorine membrane because this membrane cannot be stretched. Therefore, dissolved

2.3.2 STEP 2 - INSTALLING THE PROBES

Remove the calibration cup from your sonde by hand as shown below to expose the bulkhead.

TRANSPORT CU

BULKHEAD W ITH
PROBE PO RT PLU G S

REMOVING THE PORT PLUGS

Using the long extended end of the probe installation tool supplied in the YSI 6570
Maintenance Kit, remove the port plugs. Save all the port plugs
for possible future use.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-10

Sondes Section 2

There are a variety of probe options for the sondes. The figures below
and to the right illustrate the uses of the common tool for port plug
removal. Note that this tool will also be used to install the various
probes.

If the tool is misplaced or lost, you may use 7/64” and 9/64” hex keys as
substitutes.

DO, COND., &
pH/ORP PO RT

OPTIC
PORT PLUG

PLUGS

INSTALLATION

TOOL

INSTALLATION

NOTE: You may need pliers to remove the ISE port

plugs, but do not use pliers to tighten the ISE probes.
Hand-tighten only.

Now refer to the figures below to find the probe locations
in your sonde.

600DW-B SONDE BULKHEAD

 6572 Free Chlorine = 3-pin connector

 6560 Conductivity/Temperature = 6-pin connector
 6561 pH = 4-pin connector
 6565 Combo pH/ORP = 4-pin connector
 6566 Fouling Resistant pH//ORP = 4 pin connector
 6561FG pH (Flat Glass) = 4-pin connector
 6565FG pH/ORP (Flat Glass) = 4-pin connector

TOOL

FREE
CHLORINE
6572

ISE PORT PLUG

CONDUCTIVITY/
TEMPERATURE
6560

ALL ISE
PROBES

PLIERS

(SLI P JA WS)

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-11

Sondes Section 2

6920DW SONDE BULKHEAD

 6572 Free chlorine probe = 3-pin connector
 6560 Conductivity/Temperature = 6-pin connector
 6561 pH = 4-pin connector
 6565 Combo pH/ORP = 4-pin connector
 6566 Fouling Resistant pH//ORP = 4 pin connector
 6561FG pH (Flat Glass) = 4-pin connector
 6565FG pH/ORP (Flat Glass) = 4-pin connector
 6882 Chloride Probe = leaf spring connector
 6883 Ammonium Probe = leaf spring connector
 6884 Nitrate Probe = leaf spring connector
 6026 Turbidity Probe, Wiping = 8 pin connector
 6136 Turbidity Probe, Wiping = 8 pin connector
 6025 Chlorophyll Probe, Wiping = 8 pin connector
 6130 Rhodamine WT Probe, Wiping = 8 pin connector

ISE1/ISE2

pH/ORP

TURBIDITY
CHLOROPHYLL
RHODAMINE WT

ISE3

COND/TEMP

3

4

ISE4

FREE

E

CHLORIN

MOUNTING SCREW

1 OF 2

5

ISE5

LUBRICATE O-RINGS

Apply a thin coat of O-ring lubricant, supplied in the YSI 6570 Maintenance Kit, to the O-rings on the
connector side of each probe that is to be installed.

CAUTION: Make sure that there are NO contaminants
between the O-ring and the probe. Contaminants that are present

LUBRICATE O -RINGS

under the O-ring may cause the O-ring to leak
when the sonde is deployed.

NOTE: Before installing any probe into the sonde bulkhead, be sure that the probe port is free of

moisture. If there is moisture present, you may use compressed air to blow out the remaining moisture.

INSTALLING THE TURBIDITY, CHLOROPHYLL, AND RHODAMINE WT PROBES

If you have a turbidity, chlorophyll, and/or rhodamine WT probe, it is recommended that the optical
sensors be installed first. If you are not installing one of these probes, do not remove the port plug, and go
on to the next probe installation.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-12

Sondes Section 2

E

All optic probes, 6026 and 6136 turbidity, 6025

OPTIC

chlorophyll, and 6130 rhodamine WT are installed in the
same way. Install the probe into the center port, seating the

PROBE

pins of the two connectors before you begin to tighten.
Tighten the probe nut to the bulkhead using the short
extended end of the tool supplied with the probe. Do not
over-tighten.

INSTALLATION

TOOL

CAUTION: Be careful not to cross-thread the probe nut.

Note that the YSI 6920DW sondes can accept only a single
turbidity, chlorophyll, or rhodamine WT probe.

INSTALLING THE FREE CHLORINE PROBE, CONDUCTIVITY/TEMP AND pH/ORP PROBES

Insert the probe into the correct port and gently rotate the
probe until the two connectors align.

The probes have slip nuts that require a small probe
installation tool to tighten the probe. With the connectors
aligned, screw down the probe nut using the long extended
end of the probe installation tool. Do not over-tighten.

CAUTION: Do not cross thread the probe nut.

PROBE INSTALLATION

TOOL

INSTALLING THE ISE PROBES

The Ammonium, Nitrate and Chloride ISE probes do
nuts and should be installed without tools. Use only your fingers
to tighten. Any ISE probe can be installed in any of the three
ports labeled “3”, “4”, and “5” on the sonde bulkhead. Be sure to
remember which sensor was installed in which port so that you
will later be able to set up the sonde software correctly.

not have slip

IMPORTANT: Make sure that the probe nut or probe body
of the ISE probes are seated directly on the Sonde Bulkhead.
This will ensure that connector seals will not allow leakage.

SCREW IN AND TIGHTEN WITH FINGERS.

PROBE BODY TO SEAT

NITRATE OR AMMONIUM

INSERT ISE PROBE,

ON BULKHEAD

PROBE NUT TO SEAT

ON BULKHEAD

CL2 PROB

ISE PROBE

ISE PROBE

DO PROBE

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-13

Sondes Section 2

INSTALLING THE PROBE GUARD

Included with each sonde is a probe guard although customers will seldom use this item for drinking water
studies. The probe guard protects the probes during some calibration procedures and should be used if the
sonde were ever used in field studies that did not involve the flow cell. Once the probes are installed,
install this guard by aligning it with the threads on the bulkhead and turn the guard clockwise un til secure.

CAUTION: Be careful not to damage the free chlorine membrane during installation of the probe guard.
The figure below shows the YSI 6920DW probe guard; the guard for the 600DW-B is similar.

TURN CLOCKWISE BY

HAND TO SECURE

PROBE GUARD

BULKHEAD

(PROBES INSTALLED)

2.3.3 STEP 3 - POWER

The YSI 6920DW and 600DW-B sondes have internal batteries or can run on external power.

In addition, if you have purchased a YSI 650 MDS display/logger or 6500 Process Monitor, attaching your
sonde to these instruments will allow your sonde to be powered from the batteries of the 650 or the AC
power of the 6500.

POWER FOR LAB CALIBRATION

Using a YSI 6038 (110 VAC) or 6651 (100-240VAC) Power Supply to power the sondes in the lab is
often convenient and extends battery life. In addition, AC power will likely be available for many drinking
water studies and the 6651/6038 may be the most efficient method of providing power for long term
monitoring studies. After attaching the four-pin connector from the power supply to the instrument cable
(see below), simply plug the power supply into the appropriate AC outlet.

See Section 2.2, Connecting Your Sonde, for specific information on cables, adapters and power supplies
required for connecting your sonde to vario us de vi ces.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-14

Sondes Section 2

θ

θ

θ

θ

θ

The system configuration best suited for
initial setup is shown in the figure at the
right.

Power Supply*
6651 230V AC
6038: 110 VAC

Sonde to Lab Computer

DB-9

6095B

Adapter

MS-8

INSTALLING BATTERIES

*

Field Cable

Sonde

Not r e q u ir e d if you use
sonde battery power.

++

--

YSI 6920

YSI 6920

Yo u w ill n e ed ...

Sonde
Field Cable
Computer with Com Port
6095B MS-8/DB-9 Adapter
Power Supply *

The 6920DW and 600DW-B can also be powered by internal batteries and a set of batteries is supplied
with each of these sondes. See the sections below for instructions for battery installation

INSTALLING BATTERIES INTO THE YSI 600DW-B

To install 4 AA-size alkaline batteries into the sonde, refer to the following directions and the figure below.

Grasp the cylindrical battery cover and unscrew by hand in a counterclockwise direction. Then slide the
battery lid up and over the bulkhead connector. Insert batteries, paying special attention to polarity.
Labeling on the battery compartment posts describes the orientation. It is usually easiest to insert the
negative end of battery first and then “pop” the positive terminal into place.

Check the O-ring and sealing surfaces for any contaminants
that could interfere with the O-ring seal of the battery
chamber.

Lightly lubricate the o-ring on the outside of the battery
cover. DO NOT lubricate the internal o-ring.

Return the battery lid and tighten by hand.

DO NOT OVER-TIGHTEN.

BULKHEAD
CONNECTOR
WITH CAP

SONDE BODY
(NOT SHOWN)

BAIL

SCREW ON
BATTERY CAP

AA BATTERIES x 4

(NOTE POLARITY)

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INSTALLING BATTERIES INTO THE 6920DW

To install the 8 AA-size alkaline batteries into the sonde, refer to
the following directions and the adjacent figures.

BULKHEAD CONNECTOR
WITH CAP

Position the bail so that it is perpendicular to the sonde and use it as
a lever to unscrew the battery cap by hand. Then slide the battery lid
up and over the bulkhead connector.

Insert batteries, paying special attention to polarity. Labeling on the
top of the sonde body describes the orientation.

BATTERY CAP

BAIL

SONDE BODY

GRASP BAIL WITH HAND.

TURN COUNTERCLOCKW ISE TO LOOSEN.

F

Check the O-rings and sealing surfaces for any contaminants that could interfere with the seal of the battery
chamber.

Lightly lubricate the o-rings on the bottom of the threads and on the
connector stem as shown in the figure at the right.

BATTERY CAP

BAIL

Return the battery lid and tighten by hand. DO NOT OVER­TIGHTEN.

BULKHEAD

CONNECTOR

-

+

+

-

O-RINGS

2.3.4 STEP 4 - CONNECTING A FIELD CABLE

As shown in the picture at left, the 6920DW and
600DW-B have a sonde-mounted cable connector
for attachment of cables. Two types of cables are
available – a “field cable” in various lengths up to
200 feet and a short 6067B laboratory calibration
cable.

To attach a field cable to the sonde connector,
remove the waterproof cap from the sonde
connector and set it aside for later reassembly
during deployment or storage. Then connect your
field cable to the sonde connector.

A built-in “key” will ensure proper pin alignment.
Rotate the cable gently until the “key” engages and
then tighten the connectors together by rotating
clockwise. Attach the strain relief connector to the
sonde bail. Rotate the strain relief connector nut to
close the connector's opening.

FIELD CABLE
CONNECTOR

REMOVE

WATERPROOF CAP

SONDE

CONNECTOR

STRAIN RELIEF

CONNECTOR

BAIL

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Sondes Section 2

The other end of the field cable is a military-style 8-pin connector (MS-8). Through use of a YSI 6095B
MS-8 to DB-9 adapter, the sonde may be connected to a computer for setup, calibration, real-time
measurement, and uploading files.

This MS-8 connector also plugs directly into the 650 MDS display/logger or the 6500 Process Monitor.
These instruments contain microprocessors with software which provides the ability to set up and calibrate
your sonde sensors in a similar way to that of a terminal interface to a PC.

As an alternative to the field cable for interface to a PC, you may use a YSI 6067B calibration cable for
laboratory interaction with the sonde. In this case, simply plug the proper end of the cable into the sonde
connector and attach the DB-9 connector of the cable to the Com port of your computer. Note that the
6067B will not interface to a 650 Display/Logger of 6500 Process Monitor

For drinking water studies involving only internal logging of data or real-time display of data on a PC using
EcoWatch for Windows software, the 6067B may be the cable of choice.

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Sondes Section 2

2.4 ECOWATCH FOR WINDOWS -GETTING STARTED

This section will describe how to get started with EcoWatch for Windows, but detailed information is
provided in
software. It is recommended that you thoroughly read Section 4 or use the Help function for a
comprehensive understanding of EcoWatch for Windows.

2.4.1 INSTALLING ECOWATCH FOR WINDOWS

EcoWatch for Windows software must be used with an IBM-compatible PC with a 386 (or better)
processor. The computer should also have at least 4MB of RAM and Windows Version 3.1 or later.

Place the EcoWatch for Windows compact disk in your CD ROM drive. Select

d:\setup.exe at the prompt. Press Enter or click on “OK” and the display will indicate that EcoWatch is

proceeding with the setup routine. Simply follow the instructions on the screen as the installation proceeds.

2.4.2 RUNNING ECOWATCH FOR WINDOWS

Section 4, EcoWatch for Windows, or a convenient Windows Help section that is part of the

Start, then Run and type

To run EcoWatch for Windows, simply select the EcoWatch icon on your desktop or from the Windows
Program Menu. For help with the EcoWatch program, see
the software.

Section 4, EcoWatch or use the Help section of

2.4.3 ECOWATCH FOR WINDOWS SETUP

To setup the EcoWatch software for use with a sonde, select the sonde icon
the

proper Com port (1 or 2) to which your sonde is connected. If the default setting is correct, it does not

need to be changed. Click “

From the
it is not, select 9600 from the list and press

From the
for the EcoWatch for Windows menus.

Comm Menu, select the Settings option to check the baud rate. The baud rate should be 9600. If

Settings Menu, select the Font/Color and Background Color options to choose a color scheme

OK” to open a terminal window.

Enter.

on the toolbar, and then

2.5 SONDE SOFTWARE SETUP

There are two sets of software at work in any YSI environmental monitoring system. One is resident in the
sonde and the other is associated with the interface device (a PC, 650 Display/Logger, or 6500 Process
Monitor). If you are using a PC for your initial sonde setup as recommended, you will be using EcoWatch
for Windows software which was installed as described above and you should follow the instructions in the
section below to prepare your DW sonde for use. If you are using a 650 for sonde interface, see Section 5
for setup and interface instructions. If you are using a 6500 for sonde interface, see the manual which was
supplied with this instrument.

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Sondes Section 2

For PC-based sonde interface, run EcoWatch for Windows and click on the sonde icon, from the
toolbar Then select the proper Com port and confirm by clicking
below will appear indicating connection to the sonde as shown in the figure below. Type “

# sign, press Enter, and the sonde Main menu will be displayed.

OK. A window similar to that shown

Menu” after the

If your sonde has previously been used, the
communication is established. In this case simply proceed as described below. You will not be required to

Menu”.

type “

If you are unable to establish interaction with the sonde, make sure that the cable is properly connected. If
you are using external power, make certain that the YSI 6038 or 6651 power supply or other 12 vdc source
is properly working. Recheck the setup of the Com port and other software parameters. Also refer to

Section 6, Troubleshooting.

The sonde software is menu-driven. You select functions by typing their corresponding numbers. You do
not need to press

Enter after choosing a selection. Type the 0 or Esc key to return to the previous menu.

Main menu (rather than the # sign) may appear when

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Sondes Section 2

Sonde Main Menu

------------------Main----------------­1-Run 5-System
2-Calibrate 6-Report
3-File 7-Sensor
4-Status 8-Advanced

Select option (0 for previous menu):

Sonde Menu Flow Chart

SONDE MENU FLOW CHART

Sonde

1. Run

2. Calibrate

3. File

4. Status

5. System

6. Report

7. Sensor

8. Advanced

1. Discrete sample

2. Unattended sample

Date and Time
Battery Voltage
Available Memory
Logging Status

1. Date & Time

2. Comm Setup

3. Page Length

4. Instrument ID

5. SDI-12 Addr ess

1. Cal Constants

2. Setup

3. Sensor

4. Data Filter

1. Conductivity

2. DO %

3. DO mg/L

4. Others

1. (Y) Date

2. (Y) Time

3. (Y) Temp, C

4. ( ) Temp, F

MORE

1. Directory

2. Upload

3. Quick Upload

4. View File

5. Quick View File

6. Delete All Files

7. Test Memory

1. (Y) Temp

2. (Y) Cond

3. (Y) DO

4. ( ) ISE1 pH

MORE

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Sondes Section 2

SYSTEM SETUP

At the Main menu, select

System. The System Setup menu will be displayed.

System Setup Menu

1-Date & time
2-Comm setup
3-Page length=25
4-Instrument ID=YSI Sonde
5-Circuit board SN:00003001
6-GLP filename=00003001
7-SDI-12 address=0
8-(*)English
9-( )Fran?ais
A-( )Deutsch

Select option (0 for previous menu):

Select 1-Date & time. An asterisk will appear next to each selection to confirm the entry. Press 4 and 5 to
activate the date and time functions. Pay particular attention to the date format that you have chosen when
entering date. You must use the 24-hour clock format for entering time. Option 4- ( ) 4 digit year may be
used so that the date will appear with either a two or four digit year display. If you do not enter the correct
year format (8/30/

98 for 2-digit, 8/30/1998 for 4 digit) your entry will be rejected.

-----------Date & time setup----------­1-(*)m/d/y 4-( )4 digit year
2-( )d/m/y 5-Date=08/11/98
3-( )y/m/d 6-Time=11:12:30

Select option (0 for previous menu):

4-Instrument ID from the System setup menu to record the instrument ID number (usually the

Select
instrument serial number), and press
serial number of your sonde. This will make sure that any data that is collected is associated with a
particular sonde. Note that the selection
resident in your sonde (not the entire system as for Instrument ID). Unlike the
cannot change the
explained in Section 2.9.5

Circuit Board SN. The 6-GLP filename and 7-SDI-12 address selections will be

Enter. A prompt will appear which will allow you to type in the

5-Circuit Board SN shows the serial number of the PCB that is

Instrument ID, the user

Press Esc or 0 to return to the System setup menu.

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Sondes Section 2

At the bottom of the menu choose the language you prefer for the sonde software. For example, press

7- ( ) English to use the sonde with English menus.

1-Date & time
2-Comm setup
3-Page length=25
4-Instrument ID=YSI Sonde
5-Circuit board SN:00003001
6-GLP filename=00003001
7-SDI-12 address=0
8-(*)English
9-( )Fran?ais
A-( )Deutsch

Select option (0 for previous menu):

Then press

Esc or 0 again to return to the Main menu.

------------------Main----------------­1-Run 5-System
2-Calibrate 6-Report
3-File 7-Sensor
4-Status 8-Advanced

Select option (0 for previous menu):

ENABLING SENSORS

To activate the sensors that are in your sonde, select

Sensor from the Sonde Main menu.

------------Sensors enabled-----------­1-(*)Time
2-(*)Temperature
3-(*)Conductivity
4-(*)Free Cl2
5-(*)ISE1 pH
6-(*)ISE2 Orp
7-(*)ISE3 NH4+
8-(*)ISE4 NO3­9-( )ISE5 NONE
A-(*)Turbidity 6026

Select option (0 for previous menu):

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Note that the exact appearance of this menu will vary depending upon the sensors that are available on your
sonde. Enter the corresponding number to enable the sensors that are installed on your sonde. An asterisk
indicates that the sensor is enabled.

When selecting any of the ISE or Optical ports, a submenu will appear. When this occurs, make a selection
so that the sensor corresponds to the port in which the sensor is physically installed. Only ORP can be
enabled as ISE2. Optic T and Optic C generate a submenu on selection. Each optical port can have one of
four probes (6026 turbidity, 6136 turbidity, chlorophyll, or rhodamine WT) installed as indicated b y the
submenus.

After all installed sensors have been enabled, press

Esc or 0 to return to the Main Menu.

ENABLING PARAMETERS

In order for a specific parameter to be displayed:

1. The sensor must first be enabled as described above.

2. That parameter must be activated in the Report Setup menu described below.

Select Report from the Main menu. A Report Setup menu similar to the one shown below will be
displayed.

--------------Report setup------------­1-(*)Date m/d/y D-(*)Orp mV
2-(*)Time hh:mm:ss E-(*)NH4+ N mg/L
3-(*)Temp C F-( )NH4+ N mV
4-(*)SpCond mS/cm G-( )NH3 N mg/L
5-( )Cond H-(*)NO3- N mg/L
6-( )Resist I-( )NO3- N mV
7-( )TDS J-(*)Cl- mg/L
8-( )Sal ppt K-( )Cl- mV
9-(*)Cl2 mg/L L-(*)Turbid NTU
A-(*)Cl2chrg
B-(*)pH
C-( )pH mv

Select option (0 for previous menu):

Note that the exact appearance of this menu will vary depending upon the sensors that are available and
enabled on your sonde. The asterisks (*) that follow the numbers or letters indicate that the parameter will
appear on all outputs and reports.
to the parameter.

Note also that since a 6026 turbidity probe was selected in the Sensor menu above, the units of turbidity are
presented as “turbid NTU”. If a 6136 turbidity probe had been selected, the units of turbidity would be
presented as “turbid+ NTU”. This new designation is designed to differentiate the data from the two
sensors types in later analysis.

To turn a parameter on or off, type the number or letter that corresponds

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Sondes Section 2

For parameters with multiple unit options such as temperature, conductivity, specific conductance,
resistivity and TDS, a submenu will appear as shown below for temperature, allowing selection of desired
units for this parameter.

--------------Select units------------­1-(*)NONE
2-( )Temp C
3-( )Temp F
4-( )Temp K

Select option (0 for previous menu): 2

After configuring your display with the desired parameters, press

Esc or 0 to return to the Main menu.

CHECKING ADVANCED SETTINGS

Select

Advanced from the Main menu. The following menu will be displayed.

----------------Advanced-------------­1-Cal constants
2-Setup
3-Sensor
4-Data filter

Select option (0 for previous menu):

Select

Setup from the Advanced menu.

-------------Advanced setup----------­1-(*)VT100 emulation
2-( )Power up to Menu
3-( )Power up to Run
4-( )Comma radix
5-(*)Auto sleep RS232
6-(*)Auto sleep SDI12
7-( )Multi SDI12
8-( )Full SDI12

Select option (0 for previous menu): 0

Make sure that, other than Auto sleep RS232, all entries are activated or deactivated as shown above.

For sondes which will be used in sampling studies where the user is present and observes readings in real-

Auto sleep RS232 should usually be “off’. For sondes that will be used in unattended monitoring

time,

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Sondes Section 2

studies, Auto sleep RS232 should usually be “on”. This is described in detail in Section 2.9, Sonde Menu.
When this setup is verified, press

3-Sensor from the Advanced menu and make certain that the entries are identical to those shown

Select
below.

Esc or 0 to return to the Advanced menu.

------------Advanced sensor----------­1-TDS constant=0.65
2-Cl2 temp co %/C=2.5
3-Cl2 warm up sec=60
4-( )Wait for Cl2
5-Wipes=1
6-Wipe int=1
7-SDI12-M/wipe=1
8-Turb temp co %/C=0.3
9-(*)Turb spike filter
A-Chl temp co %/C=0
B-( )Chl spike filter

Select option (0 for previous menu):

The display under
sensors that are installed in your unit. For example, if you do not have a chlorophyll probe, the last two
entries (which are relevant only to chlorophyll) will not appear.

When this setup is verified, press
the choices in the Advanced menu, see
menu.

3-Sensor may be different from the one shown in the example above, depending on the

Esc or 0 to return to the Advanced menu. For a detailed explanation of

Section 2.9.8, Advanced. Press Esc or 0 to back up to the Main

------------------Main----------------­1-Run 5-System
2-Calibrate 6-Report
3-File 7-Sensor
4-Status 8-Advanced

Select option (0 for previous menu):

The sonde software is now set up and ready to calibrate and run.

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Sondes Section 2

2.6 GETTING READY TO CALIBRATE

2.6.1 INTRODUCTION

HEALTH AND SAFETY

Reagents that are used to calibrate and check this instrument may be hazardous to your health. Take a
moment to review health and safety information in Appendix A of this manual. Some calibration standard
solutions may require special handling.

PREPARING THE 6572 FREE CHLORINE PROBE FOR CALIBRATION

Before calibration of the free chlorine sensor can be carried out, it needs to be “activated” according to the
following instructions:

• Remove the 6572 free chlorine probe from the sonde bulkhead (if it has been installed previously).

Make certain that a membrane has been installed correctly as documented in Section 2.3 above.

• Prepare a diluted solution of commercial chlorine bleach consisting of 1 part bleach to 200 parts

purified water (distilled or deionized). For example, mix 1 mL of chlorine bleach with 200 mL of
purified water.

• Place the free chlorine probe in the diluted bleach and leave for approximately 60 seconds.

CAUTION: Do not leave the probe in bleach for longer than 1 minute or the break-in time for the
probe will be excessive.

• Rinse the sensor tip and membrane with purified water and set aside in a beaker of tap (NOT deionized

or distilled) water. DO
TIME.

Now follow the instructions below for calibration of the other sensors.

CONTAINERS NEEDED TO CALIBRATE A SONDE

The calibration cup that comes with your sonde serves as a calibration chamber for all calibrations except
free chlorine and minimizes the volume of calibration reagents required. However, if you are using the
6026 or 6136 turbidity, the 6025 chlorophyll, or the 6130 rhodamine WT probes, you should visually verif y
proper movement of the wiper mechanism before beginning the calibration procedures.

Instead of the calibration cup, you may use laboratory glassware to perform many calibrations. If you do
not use a calibration cup that is designed for the sonde, you are cautioned to do the following:

9 Perform all calibrations with the Probe Guard installed. This protects the probes from possible physical

damage.

9 Use a ring stand and clamp to secure the sonde body to prevent the sonde from falling over. Much

laboratory glassware has convex bottoms.

NOT REINSTALL THE PROBE IN THE SONDE BULKHEAD AT THIS

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Sondes Section 2

9 Insure that all sensors are immersed in calibration solutions. Many of the calibrations factor in

readings from other probes (e.g., temperature probe). The top vent hole of the conductivity sensor must
also be immersed during calibrations.

CALIBRATION TIPS

1. The key to successful calibration is to insure that the senso rs are completely

submersed when calibration values are entered. Use recommended volumes
when performing calibrations.

2. For maximum accuracy, use a small amount of previously used calibration

solution to pre-rinse the sonde. You may wish to save old calibration
standards for this purpose.

3. Fill a bucket with ambient temperature water to rinse the sonde between

calibration solutions or perform the calibration near a sink where the probes
can be rinsed from the tap.

4. Have several clean, absorbent paper towels or cotton cloths available to dry

the sonde between rinses and calibration solutions. Shake the excess rinse
water off of the sonde, especially when the probe guard is installed. Dry off
the outside of the sonde and probe guard. Making sure that th e sonde is dry
reduces carry-over contamination of calibrator solutions and increases the
accuracy of the calibration.

5.

If you are using laboratory glassware for calibration, you do not need to

remove the probe guard to rinse and dry the probes between calibration
solutions. The inaccuracy resulting from simply rinsing the probe
compartment and drying the outside of the sonde is minimal.

6. Make certain that port plugs are installed in all ports where probes are not

installed. It is extremely important to keep these electrical connectors dry.

USING THE CALIBRATION CUP

Follow these instructions to use the calibration cup for calibration procedures with the 6920DW and
600DW-B sondes..

9 Ensure that a gasket is installed in the gasket groove of the calibration cup bottom cap, and that the

bottom cap is securely tightened.
portions of the bottom cap and tube.

9 Remove the probe guard, if it is installed.
9 Inspect the installed gasket on the sonde for obvious defects and if necessary, replace it with the extra

gasket supplied.

9 Screw cup assembly into place on the threaded end of sonde and securely tighten. N

tighten as this could cause damage to the threaded portions of the bottom cap and tube.

9 Sonde calibration can be accomplished with the sonde upright or upside down. A separate clamp and

stand, such as a ring stand, is required to support the sonde in the inverted position.

Note: Do not over-tighten as this could cause damage to the threaded

ote: Do not over

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Sondes Section 2

NOTE CAREFULLY: When calibrating a turbidity sensor for use with the 6920DW, you can use either the

standard calibration cup (with certain qualifications) or an optional extended length calibration cup. Please see
the section below which describes the special calibration recommendations for this sensor.

RECOMMENDED VOLUMES OF CALIBRATION REAGENTS

The approximate volumes of the reagents are specified below for both the upright and upside down
orientations.

Table 1 6920DW Sonde

Probe to Calibrate Upright Upside Down

Conductivity 200 mL 200 mL
pH/ORP 100 mL 250 mL

ISE 125 mL 275 mL
Turbidity, Chlorophyll, Rhodamine WT

w/ Standard Calibration Cup*
Turbidity, Chlorophyll, Rhodamine WT

w/ Extended Calibration Cup*

Table 2 600DW-B Sonde

Probe to Calibrate Upright Upside Down

Conductivity 50 mL 50 mL
pH/ORP 25 mL 50 mL
* See section below for special instructions dealing with calibration of the turbidity sensor with the two
types of calibration cup.

40 mL N/A

220 mL N/A

CALIBRATION OF THE 6136 TURBIDITY SENSOR

When used with the 6920DW sonde, the 6136 can be calibrated using two types of vessels:

• The standard calibration cup which was supplied with the sonde and which minimizes the volume of

calibration suspension required.

• An extended calibration cup which is designed to provide both the greatest accuracy and convenience,

but which requires the use of higher volumes of calibration suspension.

If you choose to calibrate with the standard calibration cup, you should enga ge only ONE THREAD when
screwing the calibration cup onto the sonde in order to keep the turbidity probe face as far as possible from
the calibration cup bottom to avoid interference. Even with these techniques, there will still be a small
interference from the bottom of the calibration cup that will cause your field turbidity readings to appear up
to 1 NTU lower than the actual reading. In many cases, this small error is negligible in surface water
studies, but can be important for drinking water studies where turbidity is generally very low. For
example, a true turbidity of 0.5 NTU could appear as a value of –0.5 NTU for drinking water and this is a
significant error.

The easiest way to avoid this error due to interference from the bottom of the calibration cup is to purchase an
optional, extended-length calibration cup for your 6920DW sonde (YSI Item Number 655488). Use of these
calibration cups completely eliminates bottom interference even when the calibration cup is completely
threaded onto the sonde. The only drawback to the use of the extended length cup is that its use requires
significantly higher volumes of calibration suspension. This factor is generally of no concern if you are

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Sondes Section 2

reusing the standard, but can be significant if the standard must be discarded after use according to your
protocol.

For drinking water studies with the 6920DW and 600DW-B sondes where the turbid ity is usually very low,
YSI highly recommends the second method described above, i.e. the one utilizing the extended length
calibration cups. If you wish to purchase these lengthened calibration cups, the parts to order are shown
below and listed in the

Extended Calibration Cup Sleeve for the 6920DW – YSI Item # 655488

Appendix C Accessories and Calibration Standards:

2.6.2 CALIBRATION PROCEDURES

CAUTION: Before beginning the calibration of any sensors, make certain that the 6572 free chlorine
probe is REMOVED from the sonde bulkhead and stored in purified water. Then be sure to install
a port plug in the vacated free chlorine port. Exposure of the free chlorine sensor to some of the
reagents used to calibrate the other sensors can temporarily compromise the performance of the
sensor.

The following calibration procedures are for the most commonly used sensors. For detailed information on
all calibration procedures, refer to Section 2.9.2, Calibrate.

To ensure more accurate results, you can rinse the calibration cup with water, and then rinse with a small
amount of the calibration solution for the sensor that you are going to calibrate. Discard the rinse solution
and add fresh calibrator solution. Use tables 1-4 to find the correct amount of calibrator solution.

1. Carefully immerse the probes into the solution and rotate the calibration cup to engage several

threads. YSI recommends supporting the sonde with a ring stand and clamp to prevent the sonde
from falling over.

2. With the proper cable, connect the sonde to a PC, access EcoWatch for Windows and proceed to

the Main menu (for information on how to run EcoWatch for Windows software, see

2.4.2, Running EcoWatch Software)

---------------Calibrate-------------­1-Conductivity 6-ISE3 NH4+
2-Free Cl2 7-ISE4 NO3-
3-Pressure-Abs 8-Optic T-Turbidity-6026
4-ISE1 pH 9-Optic C-Chlorophyll
5-ISE2 ORP

Select option (0 for previous menu):

3. Note that the exact appearance of this menu will vary depending upon the sensors that are
available and enabled on your sonde. To select any of the parameters from the Calibrate menu,
input the number that is next to the parameter. Once you have chosen a parameter, some of the
parameters will have a number that appears in parentheses. These are the default values and will

Section

. From the sonde Main menu, select 2-Calibrate.

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Sondes Section 2

be used during calibration if you press Enter without inputting another value. Be sure not to
accept default values unless you have assured that they are correct. If no default value appears,
you must type a numerical value and press

4. After you input the calibration value, or accept the default, pr ess
appear on the screen. Carefully observe the stabilization of the readings of the parameter that is
being calibrated. When the readings have been stable for approximately 30 seconds, press
to accept the calibration. The calibrated value is bolded on the example screen on the following
page.

5. Press Enter to return to the Calibrate menu, and proceed to the next calibration.

Enter.

Enter. A real-time display will

Enter

CALIBRATION EXAMPLE

The example below for calibration of specific conductance, is designed to demonstrate the general calibration
protocol for all parameters. From the Calibrate menu, press
shown.

1-Conductivity and the following display will be

------------Cond calibration-----------

1-SpCond
2-Cond
3-Salinity

Select option (0 for previous menu): 1

Select 1-SpCond (the generally-recommended method for calibration of a conductivity sensor), t he followi ng
prompt will appear which calls for your numerical input.

Enter SpCond in mS/cm (10):

As noted above, the number in parentheses is the default value of this parameter and will be used in the
calibration if only
the calibration of all parameters, but for some sensors, such as pH, no default values are provided. In these
cases, the user must input a numerical value and then press

Enter is pressed without typing in another value. Similar prompts will be displayed duri ng

Enter.

After the calibration value is input and Enter is pressed, a real-time display similar to the following will then
appear on the screen..

Date Time Temp SpCond Sal Cl2 Cl2 chrg pH Battery
mm/dd/yy hh:mm:ss C mS/cm ppt mg/L volts

------------------------------------------------------------------------------

To calibrate, press <Enter> when the readings are stable.

05/05/97 08:39:51 20.83 9.602 8.837 1.04 8.5 7.06 10.2

Note that all parameters that hav e been enabled will appear - not just the o ne being ca librated at the mo men t.
The user should carefully observe the stabilization of the readings of the parameter that is being calibrated

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Sondes Section 2

and, when the readings are stable for approximately 30 seconds, press Enter to implement the calibration and
the following message will appear.

Calibrated. Press <Enter> to continue.

NOTE: If an ERROR message appears, begin the calibration procedure again. Be certain that the value you

enter for the calibration standard is correct. Also see Section 8, Troubleshooting for more information on
error messages.

CAUTION: Be certain to immerse the entire sonde in solution standards for calibration of all
parameters. Most calibrations require readings not only from the sensor being calibrated but also
from the temperature sensor.

Specific

start-up calibration procedures for all sensors that commonly require calibration are
provided in the following paragraphs of this section. Remember that these are basic protocols
designed to get the user up and running with your 6-series sonde. A more-detailed discussion of
sensor calibration can be found in Section 2.9.2.

CONDUCTIVITY

This procedure calibrates conductivity, specific conductance, salinity, and total dissolved solids.

Place the correct amount (see Tables 1-4) of 10 mS/cm conductivity standard (YSI 3163 is recommended)
into a clean, dry or pre-rinsed calibration cup.

Before proceeding insure that the sensor is as dry as possible. Ideally, rinse the conductivity sensor with a
small amount of standard that can be discarded. Be certain that you avoid cross-contamination of standard
solutions with other solutions. Make certain that there are no salt deposits around the pH/ORP probe,
particularly if you are employing standards of low conductivity.

Carefully immerse the probe end of the sonde into the solution. Gently rotate and/or move the sonde up
and down to remove any bubbles from the conductivity cell. The probe must be completely immersed past
its vent hole. Using the recommended volumes from the table in the previous subsection should insure that
the vent hole is covered.

Allow at least one minute for temperature equilibration before proceeding.

From the Calibrate menu, select

SpCond

standard you are using (mS/cm at 25°C) and press
appear on the screen and will change with time as they stabilize.

Observe the readings under Specific Conductance or Conductiv ity and when they show no significant
change for approximately 30 seconds, press
accepted and prompt you to press

Rinse the sonde in tap or purified water and dry the sonde.

to access the specific conductance calibration procedure. Enter the calibration value of the

Conductivity to access the Conductivity calibration procedure and then 1-

Enter. The current values of all enabled sensors will

Enter. The screen will indicate that the calibration has been

Enter again to return to the Calibrate menu.

NOTE: The YSI conductivity system is very linear over its entire 0-100 mS/cm range. Therefore, it is

usually not necessary to use calibration solutions other than the 10 mS/cm reagent recommended above for
all environmental applications from low conductivity freshwater to seawater. YSI does offer the 3161

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-31

Sondes Section 2

(1 mS/cm) and 3165 (100 mS/cm) conductivity standards for users who want to assure maximum accuracy
at the high and low ends of the sensor range. Users of the 1 mS/cm standard should be particularly careful
to avoid contamination of the reagent. In fact, because of contamination issues, YSI does not recommend
using standards less than 1 mS/cm. For example:

pH 2-POINT

Using the correct amount of pH 7 buffer standard (see Tables 1-4) in a clean, dry or pre-rinsed calibration
cup, carefully

immerse the probe end of the sonde into the solution. Allow at least 1 minute for

temperature equilibration before proceeding.

From the Calibrate menu, select ISE1 pH to access the pH calibration choices and then press 2- 2-Point.

Enter and input the value of the buffer (7 in this case) at the prompt. Press Enter and the current

Press
values of all enabled sensors will appear on the screen and change with time as they stabilize in the
solution. Observe the readings under pH and when they show no sign ificant change for approximately 30
seconds, press

After the pH 7 calibration is complete, press
the sonde in water and dry the sonde before proceedi n g to the next st ep.

Using the correct amount (see Tables 1-4) of an additional pH buffer standard into a clean, dry or pre­rinsed calibration cup, carefully
minute for temperature equilibration before proceeding.

Enter and input the value of the second buffer at the prompt. Press Enter and the current values of

Press
all enabled sensors will appear on the screen and will change with time as they stabilize in the solution.
Observe the readings under pH and when they show no significant change for approximately 30 seconds,

Enter. After the second calibration point is complete, press Enter again, as instructed on the screen,

press
to return to the Calibrate menu.

Rinse the sonde in water and dry. Thoroughly rinse and dry the calibration containers for future use.

NOTE: The majority of environmental water of all types has a pH between 7 and 10. Therefore, unless

you anticipate a pH of less than 7 for your application, YSI recommends a two point calibration using pH 7
and pH 10 buffers.

The next calibration instructions are only for the 6920DW sonde. If you do not have this sonde, you

may skip to 2.7, Taking Readings.

AMMONIUM (NH

WARNING: AMMONIUM AND NITRATE SENSORS CAN ONLY BE USED AT DEPTHS OF LESS

THAN 50 FEET (15 METERS). USE OF THE SENSORS AT GREATER DEPTHS IS LIKELY TO
PERMANENTLY DAMAGE THE SENSOR MEMBRANE.

The calibration procedures for ammonium, nitrate or chloride are similar to pH except for the reagents in
the calibration solutions. Suggested values for calibrants are 1 and 100 mg/L of either ammonium-nitrogen

-N) or nitrate-nitrogen (NO3-N). Suggested values for calibrants are 10 and 1000 mg/L of Chloride

(NH

4

-

(Cl

).

: The following procedure requires one portion of the high concentration calibrant and two portions

NOTE

of the low concentration calibrant. The high concentration solution and one of the low concentration

Enter. The display will indicate that the calibration is accepted.

Enter again, as instructed on the screen, to continue. Rinse

immerse the probe end of the sonde into the solution. Allow at least 1

+

) , CHLORIDE (Cl- ) and NITRATE (NO

4

-

) 3-POINT

3

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-32

Sondes Section 2

solutions should be at ambient temperature. The other low concentration solution should be chilled to less
than 10°C prior to beginning the procedure.

Place the proper amount of 100 mg/L standard (1000mg/l for chloride) into a clean, dry or pre-rinsed
transport cup. Carefully

immerse the probe end of the sonde into the solution. Allow at least 1 minute for

temperature equilibration before proceeding.
Select

Ammonium, Nitrate, or Chloride to access the appropriate calibration choices. Then select 3-3-

Point

. Press Enter and input the concentration value of the standard as requested. Press Enter and the

current values of all enabled sensors will appear on the screen and will change with time as they stabilize in
the solution. Observe the readings under NH4
approximately 30 seconds, press

Enter.

+

, NO3-, or Cl- . When they show no significant change for

After the first calibration point is complete, proceed as instructed on the screen, to continue. Rinse the
sonde in water and dry the sonde prior to the next step.

Place the proper amount of 1 mg/L standard for Ammonium or Nitrate (10 mg/l for Chloride) into a clean,
dry or pre-rinsed transport cup. Carefully

immerse the probe end of the sonde into the solution. Allow at

least 1 minute for temperature equilibration before proceeding.

Press

Enter and input the concentration value of the standard as requested.

Enter and the current values of all enabled sensors will appear on the screen and will change with

Press
time as they stabilize in the solution. Observe the readings under NH4
show no significant change for approximately 30 seconds, press

+

, NO3-, or Cl-.and when they have

Enter.

After the second value calibration is complete, press
1 mg/L standard (10 mg/L for the chloride) into a clean, dry or pre-rinsed calibration cup. Carefully

Enter to continue. Place the proper amount of chilled

immerse the probe end of the sonde into the solution. Allow at least 5 minutes for temperature
equilibration before proceeding.

Enter and input the concentration value of the standard as requested.

Press
Press

Enter and the current values of all enabled sensors will appear on the screen and will change with

time as they stabilize in the solution. Observe the readings under NH4
no significant change for approximately 30 seconds, press
After the third value calibration is complete, press

Enter to return to the Calibrate menu.

Enter.

+

, NO3-. or Cl-. and when they show

Thoroughly rinse and dry the calibration cups for future use.

CALIBRATION TIP: HOW TO AVOID DRIFT DURING CALIBRATION OF THE NH4+, NO3- AND

Cl- PROBES AFTER pH CALIBRATION. Exposure to the high ionic content of pH buffers can cause a
significant, but temporary, drift in these ISE pro bes (ammonium, nitrate and chloride probes). Therefore,
when calibrating the pH probe, YSI recommends that you use one of the following methods to minimize
errors in the subsequent readings:

¾ Calibrate pH first, immersing all of the probes in the pH buffers. After calibrating pH, place the

probes in 100 mg/L nitrate or ammonium standard or 1000 mg/L chloride standard and monitor the
reading. Usually, the reading starts low and may take as long as 30 minutes to reach a stable value.
When it does, proceed with the calibration.

¾ When calibrating pH, remove ISE modules from the sonde bulkhead and plug the ports. After pH

calibration is complete, replace the ISE sensors and proceed with their calibration with no stabilization
delay.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-33

Sondes Section 2

TURBIDITY 2-POINT

Select Optic T (or Optic C) –Turbidity-6136 from the Calibrate Menu and then 2-2-Point.

NOTE: Before calibrating your 6136 turbidity sensor, pay particu lar attention to the following cautions:

• To properly calibrate YSI turbidity sensors, you MUST use standards that have been prepared

according to details in Standard Methods for the Treatment of Water and Wastewater (Section 2130 B).
Acceptable standards include (a) formazin prepared according to Standard Methods; (b) dilutions of
4000 NTU formazin concentrate purchased from Hach; (c) Hach StablCal
denominations; and (d) AMCO-AEPA standard s prepared specifically for the 6026 and 6136 by either
YSI or approved vendors who are listed on the YSI website (www.ysi.com). STANDARDS FROM
OTHER VENDORS ARE
USE WILL LIKELY RESULT IN BOTH CALIBRATION ERRORS AND INCORRECT FIELD
READINGS.

For AMCO-AEPA standards, the value entered by the user during the calibration protocol is

•

DIFFERENT depending on which sensor (6026 or 6136) is being calibrated. This reflects the
empirically determined fact that 6026 and 6136 sensors that have been calibrated to the same value in
the primary standard formazin, will have different responses in suspensions of the AEPA-AMCO
beads. This effect is likely due to the larger optical cell volume of the 6136. Thus, for example, the
label of the YSI 6073 turbidity standard bottle indicates that the value of the standard is

when used for calibration of the 6026 sensor, but 123 NTU when used to calibrate the 6136.

Note that the phenomenon of a sensor-specific formazin/AEPA-AMCO ratio is well known for sensors
other than the 6026 and 6136.

NOT APPROVED FOR THE YSI TURBIDITY SYSTEM AND THEIR

TM

standards in various NTU

100 NTU

• When calibrating a 6136 turbidity sensor you MUST use either a modified calibra tion cup arrangement, an

extended length calibration cup or laboratory glassware with the probe guard installed for the calibration.
See section 2.6.1 above for details. Failure to carry out the calibration properly can result in inaccurate
readings, particularly water of very low turbidity.

• Before proceeding with the calibration, be certain that the probe compartment of the sonde has been

cleaned and is free of debris. Solid particles from this source, particularly those carried over from past
deployments, will contaminate the standards during your calibration protocol and cause either
calibration errors and/or inaccurate field data.

• One standard must be 0 NTU, and this standard must be calibrated first.

To begin the calibration, place the correct amount (see Tables 1-5) of 0 NTU standard (clear deionized or
distilled water) into the calibration cup provided with your sonde. Immerse the sonde in the water
the value 0 NTU at the prompt, and press
you to determine when the readings have stabilized. Activate the wiper 1-2 times by pressing

Optics

as shown on the screen, to remove any bubbles. After stabilization is complete, press Enter to

“confirm” the first calibration and then, as instructed, press

Enter. The screen will display real-time readings that will allow

Enter to continue.

Dry the sonde carefully and then place the sonde in the second turbidity standard (100 NTU is
suggested) using the same container as for the 0 NTU standard. Input the correct turbidity value in
NTU, press Enter, and view the stabilization of the values on the screen in real-time. As above,
activate the wiper with the “3” key or manually rotate the sonde to remove bubbles. After the
readings have stabilized, press Enter to confirm the calibration and then press Enter to return to the
Calibrate menu.

NOTE CAREFULLY: If you are calibrating a 6136 turbidity sensor you MUST use either a modified

calibration cup arrangement or laboratory glassware with the probe guard installed for the calibration. See

. Input

3-Clean

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-34

Sondes Section 2

section 2.6.1 above for details. Failure to carry out the calibration properly can result in inaccurate readings,
particularly in water of very low turbidity.

Thoroughly rinse and dry the calibration cups for future use.

CHLOROPHYLL 1-POINT

Select Optic C -Chlorophyll from the Calibrate Menu, Chl µg/L and then 1-1 point.

NOTE:

chlorophyll concentration in µg/L, allowing quick and easy fluorescence measurements that are only semi­quantitative with regard to chlorophyll. However, the readings will reflect changes in chlorophyll from site
to site, or over time at a single site.

To increase the accuracy of your chlorophyll measurements, follow the 2-point or 3-point calibration
protocols outlined in

Before making any field readings, carefully read Section 5.12, about chlorophyll that describes practical
aspects of fluorescence measurements.

This procedure will zero your fluorescence sensor and use the default sensitivity for calculation of

Section 2.9, Sonde Menu.

To begin the calibration, place the correct amount of clear deionized or distilled water, from Tables 1, 2 and
3, into the YSI clear calibration cup provided. Immerse the sonde in the water. Input the value 0 µg/L at
the prompt, and press
when the readings have stabilized. Activate the wiper 1-2 times by pressing
the screen to remove any bubbles from the sensor. After stabilization is complete, press Enter to
“confirm” the calibration and then, as instructed, press

Thoroughly rinse and dry the calibration cups for future use. For additional information related to
calibrating the chlorophyll sensor, see Sections 5.12.

Enter. The screen will display real-time readings that will allow you to determine

3-Clean Optics as shown on

Enter to return to the Calibrate menu.

RHODAMINE WT 2-POINT

Select Rhodamine from the Calibrate Menu and then 2-2-Point.

NOTE: One standard must be 0 ug/L in rhodamine WT, and this standard must be calibrated first.

To begin the calibration, place the correct amount (see Tables 1-4) of 0 standard (clear

distilled water) into the calibration cup provided with your sonde and immerse the sonde in the water.
Input the value 0 ug/L at the prompt, and press Enter. The screen will display real-time readings that
will allow you to determine when the readings have stabilized. Activate the wiper 1-2 times by
pressing 3-Clean Optics as shown on the screen, to remove any bubbles. After stabilization is
complete, press Enter to “confirm” the first calibration and then, as instructed, press Enter to
continue.

Dry the sonde carefully and then place the sonde in the second rhodamine WT standard (100 ug/L is
recommended) using the same container as for the 0 ug/L standard. Input the correct rhodamine WT
concentration in ug/L, press Enter, and view the stabilization of the values on the screen in real-time.
As above, activate the wiper with the “3” key or manually rotate the sonde to remove bubbles. After
the readings have stabilized, press Enter to confirm the calibration and then press Enter to return to
the Calibrate menu.

deionized or

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-35

Sondes Section 2

Thoroughly rinse and dry the calibration cups for future use.

FREE CHLORINE 1-POINT

The 6572 free chlorine sensor MUST be calibrated in situ, i.e. in flowing drinking water whose free
chlorine content has been determined by an independent method. In many cases, the independent method
involves the use of the DPD Colorimetric Method as defined in Section 4500-Cl G of Standard Methods for
the Examination of Water and Wastewater. Relatively inexpensive colorimeters, such as the YSI Models
9000 and 9100 and the Hach Model 5870000, are available for carrying out the DPD method to d e termine
the free chlorine in the effluent of the flow cell associated with the 6920DW or 600DW-B drinking water
studies.

After activation of the sensor according to the instruction in Section 2.6.1 above and AFTER the calibration
of all other sensors present, reinstall the free chlorine probe in the sonde bulkhead after removing the port
plug. Make certain that no moisture is present in the port before installing the probe and, if moisture is
present from the previous calibration procedures, use compressed air to dry the port.

After installing the free chlorine sensor in the sonde bulkhead, locate the flow cell which you purchased
with your drinking water sonde and install the sond e in the flow cell according to the instruction supplied
with the flow cell and as provided below:

• Attach inlet and outlet hoses to the barbed fittings supplied with the flow cell and then insert the

fittings into the proper ports of the flow cell. Make certain that the hoses are attached very snugly
to the barb fittings to prevent any possibility of catastrophic leakage. If necessary, standard hose
clamps can be used to secure the hoses to the barbed fittings.

• Connect the inlet line of the flow cell to a drinking water tap and run the outlet line to a drain.

Make certain that the inlet line is securely attached to the tap and that the outline line will not pop
out of the drain under flow conditions.

NOTE CAREFULLY: Make certain that the inlet line is attached to the bottom fitting of the
flow cell and the

• Turn on the water with a flow rate of 500-1000 mL/minute (500 mL/min mini mu m).

• Break in the sensor by running it continuously in flowing tap water by one of the following

methods:

• If using a PC with EcoWatch for sonde interface, start a Discrete Sample study at a 4 second

interval as described below and continue the study for 2-3 hour s while displaying the readings
on the PC monitor.

• If using a 650 Display/Logger for sonde interface, choose the Sonde Run selection from the

Main menu and continue the study for 2-3 hours while displaying the readings on the 650
screen.

• If using a 6500 Process Monitor for sonde interface, simply allow the system to run for 2-3

hours while displaying the readings on the 6500 screen.

During the 2-3 hour break-in period, the Free Chlorine and Chlorine Charge readings should drop
significantly and show reasonable stability for periods of 5 minutes.

drain line is attached to the top fitting.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-36

Sondes Section 2

NOTE: At the end of the break-in period, the Cl2 Charge value should have a value between zero (0)
and 35. If the Cl2 charge value is greater than 35, a new membrane should be installed and the
activation and break-in process should be repeated.

The free chlorine system should be calibrated using the same basic methods employed for other sonde
sensors. The important thing to remember is to calibrate your sensor versus a flow cell effluent
mode which you will be using to measure free chlorine. There are four basic modes of operation as
described below with their corresponding calibration instructions:

Stand-alone applications where data is being logged only to the internal memory of the

1.

sonde at a user selected interval.

RS232 feature is activated in the
to 60 seconds in the
input the free chlorine value from the DPD analysis of the flow cell effluent, and th e unit will
automatically calibrate after the selected warm up time. This method is described in greater detail
below.

Applications where the sonde is attached to a YSI 6500 Process Monitor utilizing the Sample

2.

Advanced|Sensor menu. With these software entries in place, the user will

and Hold feature of the sonde software.
and Hold

6500 front panel simultaneously to access this menu). Then select the desired interval for data to
be sent to the 6500. Exit to the main display mode of the 6500 by pressing the
repetitively and then press the
entry, enter the value from the DPD analysis of the effluent and press
again to confirm the calibration. See Section 4 of the 6500 manual for detailed calibration
instructions.

Applications where the sonde is attached to a YSI 6500 Process Monitor and is outputting

3.

feature from the Setup sonde menu of the 6500 (Press the Esc and Enter keys of the

data continuously to the 6500.

6500 as described above and make certain that the Sample and Hold feature is
exit to the main display mode of the 6500 by pressing the
key on the 6500 front panel. Highlight the free chlorine sensor entry, enter the value from the
DPD analysis of the effluent and press
and, when stable, press the
manual for detailed calibration instructions. Note that this mode of operation is NOT
RECOMMENDED by YSI due to sensor maintenance issues which are explained in detail in
Appendix G of this manual.

For this mode of operation, make certain that the Autosleep

Advanced|Setup menu and that the Chlorine warm-up time is set

For this mode of operation, first activate the Sample

Esc key

Cal key on the 6500 front panel. Highlight the free chlo ri ne sensor

Enter. Then press Enter

For this mode of operation, access the Sonde setup menu of the

INACTIVE. Then

Esc key repetitively and press the Cal

Enter. Observe the free chlorine readings in real-time

Enter key to confirm the calibration. See Section 4 of the 6500

4. Applications where the sonde is attached to a PC and data is being displayed to the

computer monitor using the Real-Time mode of Ecowatch for Windows.

operation, your must calibrate the free chlorine sensor before activating the Real-Time EcoWatch
mode or after activating the Real-time feature of EcoWatch. Access the sonde menu after
connection of the sonde to a PC or 650 Display logger and make certain that the Autosleep RS232
feature is activated in the
seconds in the
the free chlorine value from the DPD analysis of the flow cell effluent, and the unit will
automatically calibrate after the selected warm up time. Following calibration, attach the sonde to
the PC and click on the Real-Time entry in the top toolbar (Do NOT click on the sonde icon in the
lower toolbar). The Real-Time study will start automatically with data displayed graphically on
the PC monitor and logged to the hard drive of the PC under the file name which you have
selected. See Section 4.3.5 of this manual for detailed instruction in using the EcoWatch Real­Time mode.

Details of the calibration for the simple
below. The initial instructions involving the setup of the flow cell/sonde system are also valid for
calibration in the other modes of operation.

Advanced|Sensor menu. With these software entries in place, the user will input

Advanced|Setup menu and that the Chlorine warm-up time is set to 60

Unattended sample mode of operation (#1 above) are provided

For this mode of

in the

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-37

Sondes Section 2

For the
has been underway for 2-3 hours to assure the stabilization of the free chlorine sensor in the flowing tap
water. The setup of the logging study (or Unattended Sampling) is described in detail in

UNATTENDED SAMPLING

to assure stability prior to calibration, see

To calibrate the free chlorine sensor, carry out the following steps:

Unattended sample mode, the free chlorine sensor should be calibrated AFTER a logging study

Section 2.7

and 2.9.1 Run UNATTENDED SAMPLING. To view the logged data

Section 2.9.3 File.

• Make certain that the flow of water through the flow cell is 500-1000 mL per minute (minimum

500 mL/min) and that the flow is set to the rate at which you will carry out your monitoring study.

• Interface to the sonde using a 650 display/logger, a PC running EcoWatch for Windows, or a 6500

Process Monitor. If you are using a PC or the 650 Display/Logger, select

2-Setup and make certain that Autosleep RS232 is active as shown in the box below.

8-Advanced and then

-------------Advanced setup----------­1-(*)VT100 emulation
2-( )Power up to Menu
3-( )Power up to Run
4-( )Comma radix
5-(*)Auto sleep RS232
6-(*)Auto sleep SDI12
7-( )Multi SDI12
8-( )Full SDI12

Select option (0 for previous menu): 0

• Set up your Unattended Sampling study as described above and allow readings to be logged to

internal memory for 2-3 hours. Using the
inspect the free chlorine readings and after they are stable proceed with the calibration as
described below. If you are using a 6500 Process Monitor, observe the real-time readings on the
display for 2-3 hours and make certain they are stable before proceeding with the calibration.

• Collect a sample of water from the outlet hose of the flow cell and immediately analyze it for free

chlorine using an independent method , e.g. , t he DP D method with a YSI 9100 Photometer.

• If using a PC or 650 Display/Logger for sonde interface, return to the Main menu using the

key and select

• NOTE: If using a 6500 Process Monitor for the sonde interface, perform the calibration according

to the instructions in Section 4 of the 6500 manual.

• At the prompt, input the independently determined free chlorine value and press

display similar to that shown below will appear on either the computer monitor or the 650 display.
The calibration will occur automatically after the countdown on the screen reaches zero.

2-Calibrate and then 4-Free Cl2.

File|View command as described in Section 2.9.3,

Esc

Enter. A

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-38

Sondes Section 2

===================================================================
Temp SpCond Sal DOsat Cl2 Depth pH NH4+ N NO3- N Turbid
C mS/cm ppt % mg/L feet mg/L mg/L NTU

------------------------------------------------------------------­Stabilizing: 38

• After the warm-up time is complete, the readings just before and just after calibration are

displayed. When you press

Enter, the screen returns to the Free Chlorine Calibration menu.

2.7 TAKING READINGS

After you have (1) enabled the sensors, (2) set the report to show the parameters that you want to see, and
(3) calibrated the sensors, you are now ready to take reading s .

There are two basic approaches to sampling, Discrete and Unattended. Using discrete sampling, the sonde
is connected via a communication cable to a PC or terminal. The sampling frequency is likely to be rapid
(seconds) in order to obtain a representative sampling as you move from site to site. Readings will probably
be logged to several different files.

Unattended sampling is normally done with sondes that have internal batteries as do the 600DW-B and the
6920DW. The sampling frequency is likely to be longer (minutes). A sonde is typically deployed for days
or weeks at a time, and readings will be logged to a single file. The communication cable may be
disconnected and internal battery power used to operate the sonde. Alternatively, the sonde may connect
via SDI-12 communication to a data collection platform (DCP). Sondes without batteries need to be
connected to an external power source for unattended sampling.

NOTE: The 600DW-B and 6920DW are designed around the long-term, continuous monitoring of
drinking water if flow cell applications. Thus, for most studies data will either be stored at relatively
long sample intervals (5-60 minutes) to the internal memory of the sonde using the Unattended mode
or the sonde will be attached to a 6500 Process Monitor and the data will be output in 4-20 mA or
Modbus format and stored in a SCADA system or similar external logger. Thus, while the Discrete
sample mode may be required in some set-up and troubleshooting operations, most studies will be
done using the Unattended mode.

1-Run from the Main menu to begin taking readings or to set/verify the parameters required for a

Select
study. There are two options in the Run menu as shown below.

---------------Run setup--------------­1-Discrete sample 2-Unattended sample

Select option (0 for previous menu): 1

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Sondes Section 2

DISCRETE SAMPLING

NOTE THAT THIS FUNCTION WILL NORMALLY BE USED IN DRINKING
WATER STUDIES ONLY DURING THE BREAK-IN OF THE SENSOR AFTER
RECONDITIONING.

Select 1-Discrete sample from the Run menu. The Discrete sample menu will be displayed.

------------Discrete sample-----------­1-Start sampling
2-Sample interval=4
3-File=
4-Site=
5-Open file

Select option (0 for previous menu):

Select

2-Sample Interval to type a number that represents the number of seconds between samples. The

maximum sample interval is 32767 seconds (9+ hours). The factory default sample interval is 4 seconds
and works best for most discrete sampling applications. See section 2.9.1 for more details.

Select 3-File to enter a filename with a maximum of 8 characters. This is the file to which you will log
readings.

If you started sampling without entering a filename, the default name NONAME1 will be assigned to your
file. Whenever you press 1-LOG last sample or 2-LOG ON/OFF from the menu, NONAME1 will b e
opened during sampling. If this happens, and you want to restart the file with a different name, press

5-Close file and rename the file.

Select

4-Site to assign a site name with a maximum of 31 characters. This allows you to enter the name of

the site where you are sampling.

When you select
finished logging data to the file, press

Now select

After the initial sampling time interval has passed, (e.g. 4 seconds in the example above), sequential lines
of data (similar to that shown below) will appear on the screen.

5-Open File, a file is opened and the number 5 changes to Close File. When you are

5-Close File and number 5 changes back to Open File.

1-Start sampling to start discrete sampling.

YSI Incorporated Drinking Water Monitoring Systems Operations Manual 2-40

Sondes Section 2

============================================================
Temp Sal DO% pH Depth pH NH4+ N NO3- N Turbid
C ppt %SAT feet mg/L mg/L NTU

-----------------------------------------------------------­*** 1-LOG last sample 2-LOG ON/OFF, 3-Clean optics ***

23.54 0.00 96.5 8.20 1.001 5.20 0.853 0.522 0.3
*** LOG is ON, hit 2 to turn it OFF, 3-Clean optics ***

23.53 0.00 96.5 8.20 1.001 5.20 0.856 0.520 0.3

23.53 0.00 96.5 8.20 1.000 5.20 0.854 0.521 0.3

23.53 0.00 96.5 8.20 1.000 5.19 0.852 0.522 0.3
*** 1-LOG last sample 2-LOG ON/OFF, 3-Clean optics ***

23.53 0.00 96.5 8.20 1.000 5.19 0.852 0.522 0.3
Sample logged.

The following prompt will appear just below the screen header:

1-LOG last sample, 2-LOG ON/OFF, 3-Clean optics.

By entering 1–LOG last sample, a single line of data can be logged to sonde memory and the following
message will be displayed:

By entering
displayed:

By entering
wiper will clean the optical surface
optical probe was installed and enabled.

Select

2-LOG ON/OFF, a set of data can be logged to memory and the following message will be

LOG is ON, hit 2 to turn it OFF, 3-Clean optics. Press 2 again to terminate logging.

3-Clean optics, if your unit has an optical (turbidity, chlorophyll, or rhodamine WT) probe, the

Esc or press 0 to exit discrete sampling.

Sample logged.

. The 3-Clean optics portion of the prompt will only appear if an

UNATTENDED SAMPLING

2-Unattended Sampling from the Run menu. The Unattended sample menu will be displayed. Use

Select
the following example to understand the unattended sampli ng option.

Example: You are going to deploy the sonde for 2 weeks, collecting a set of

readings every 15 minutes. You start at 6:00 PM on July 17, 1996 and end the
sampling at 6:00 PM on July 31, 1996. The site is Clear Lake, near the spillway,
and you want to log all of the readings to a single file CLRLAKE3.

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------------Unattended setup----------­1-Interval=00:15:00
2-Start date=07/17/96
3-Start time=18:00:00
4-Duration days=365
5-File=
6-Site=
7-Bat volts: 11.6
8-Bat life 25.1 days
9-Free mem 41.3 days
A-1st sample in 8.10 minutes
B-View params to log
C-Start logging

Follow the prompts on this screen to prepare your 600XLM, 6600, 6920 or 600 OMS (battery version)
sonde for unattended deployment as described below:

To verify and/or correct the time and date enter 4-Status or 5-System menu from the Main menu.
You may enter the correct date and time from either of these submenus.

 Select 1-Interval and enter the desired time between samples (e.g. 15 minutes in the screen

above). Use the 24-hour clock format to enter interval.

 Select 2-Start Date and 3-Start Time to set the time that data will begin to log to sonde

memory. If you do not make any change to these entries, then the study will automatically
begin at the next integral time interval, once you have pressed C-Start logging.

Example: If the current time is 17:20:00 and your sample interval is 15 minutes, logging

will automatically begin at 17:30:00.

It is better to start the study prior to taking the unit to the field so that you can confirm that
readings are being saved to memory. If you should desire to start the instrument at the site at 6:00
PM as noted in the above example, change the Start Time to 18:00:00.

 Select 4-Duration and set the length of the study in days. The default value is 365 days (which is

longer than most deployments). In most cases, you will either want to stop the unattended study
manually or allow the batteries to be expended. It is wise to set the duration to a value longer than the
anticipated deployment. If you cannot retrieve the sonde at the expected time due to factors beyond
your control such as weather or illness, data will continue to be acquired as long as battery power is
present.

 Select 5-File and enter a name of no more than 8 characters that will be used by your external

computer to identify the study. Be sure to use ONLY alpha/numeric characters.

 Select 6-Site and enter a site name of no more than 31 characters. This filename will appear in your

sonde file directory, but will not be used to identify the file after transfer to your computer.

 Check 7-Battery to make certain that the voltage is suitable for the length of the study that you are

about to begin. No change can be made to this item via the software.

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 Skip B-View params to log in this initial test study. This feature will be explained in detail in Section

2.9,

Sonde Menu.

After making the above entries, the sonde software will automatically calculate the expected battery life,
and the time it will take for the sonde memory to be filled. This information is displayed on the screen for
your consideration as items 8, 9, and A. If the battery life or the free memory capacity will be exceeded
sooner than the duration, you may want to make some changes to th e entries. For example, you can free up
memory in the sonde by uploading all existing data from the sonde memory to your PC and then deleting
them out of the sonde (see 3-File from Main Menu). You may want to change the batteries for longer
battery life. You can lengthen the sampling interval to extend both battery life and memory capacity.

Review the screen below, which now displays the entries made above and reflects your logging conditions.

------------Unattended setup----------­1-Interval=00:15:00
2-Start date=07/17/96
3-Start time=18:00:00
4-Duration days=365
5-File=clrlake3
6-Site=Clear Lake at Spillway
7-Bat volts: 11.6
8-Bat life 25.1 days
9-Free mem 41.3 days
A-1st sample in 4.10 minutes
B-View params to log
C-Start logging

Once you press

-------------Start logging------------­Are you sure?
1-Yes
2-No

Select option (0 for previous menu):

Select

1-Yes and the screen will change.

C-Start logging, a screen will appear to request confirmation.

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----------------Logging---------------­1-Interval=00:15:00
2-Next at 07/17/96
3-Next at 18:00:00
4-Stop at 07/31/96
5-Stop at 18:00:00
6-File=clrlake3
7-Site=Clear Lake at Spillway
8-Bat volts: 11.7
9-Bat life 25.5 days
A-Free mem 41.3 days
B-Stop logging

Select option (0 for previous menu):

The display now shows the next date and time for logging, and the stop date and time for the logging study.
Most importantly, note that the bottom command now shows B-Stop logging, a confirmation that the
logging has indeed been initiated.

The Unattended study will terminate when the duration you specified has expired or the batteries are
expended. If you want to terminate sooner, simply select

B-Stop logging. Select 1-yes and return to the Unattended setup menu.

2-Unattended sample from the Run menu, then

Stop logging?
1-Yes
2-No

Select option (0 for previous menu):

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2.8 USING ECOWATCH TO CAPTURE, UPLOAD AND
ANALYZE DATA

CAPTURE

EcoWatch for Windows can be used to capture data in real-time to your PC’s hard drive or to a floppy disk.
To utilize this function, interface the sonde to your PC via a COM port, run EcoWatch for Windows, and
follow the step by step instructions below.

1. Click on the sonde icon

2. From the

3. Make sure that the sample interval is set to the correct value. If it is not, change it to the correct

value.

4. Close the terminal window by clicking on the

EcoWatch for Windows.

5. Open the

transferred. Name the file, making sure that the name has extension
the file is in the Data subdirectory of the ECOWWIN directory.

6. Click

rate you selected. The data will be automatically plotted with autoscaling and saved as a .DAT file
at your chosen selection.

7. To terminate the study, open the

Main sonde menu press 1-Run and then 1-Discrete Sample.

Real-Time menu, click on New and select the location where you want to data

OK. After EcoWatch sets up the sonde for the study, data transfer will begin at the sample

, choose the proper Com port, and confirm.

X in the upper right hand corner. Do not close

.RT. The default location for

Real-Time menu, choose Close, and click OK.

UPLOAD DATA

EcoWatch for Windows software is reporting and plotting software for use with the YSI 6-Series sondes.
Instructions for installing this software were included in
also be used to upload and view data logged to sonde memory during either discrete or unattended
sampling.

If the sonde was deployed unattended (without a cable), clear debris and water from the bulkhead connector
cap. Then remove the cap and connect the calibration cable (or field cable and adapter) from the sonde to
your computer.

You may now retrieve data files using the following procedure. Remember these are files in the sonde
directory, not files in the EcoWatch directory.

Section 2.1, Getting Started. This program can

Run EcoWatch software on your PC and select the
interface window (like that shown below) will appear. If a “#” prompt appears instead of the Main sonde
menu, type “menu” at the prompt to generate the display as shown. Then press
handling options.

Sonde icon from the menu bar. A terminal

3-File to view data

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1-Directory to view all files currently stored in the sondes flash disk memory, the screen below

Select
shows 6 files. All data files (.dat extension) coul d be uploaded to EcoWatch for viewing or plott i ng, but
you do not need to upload all files in the directory. The file with the .glp extension contains the calibration
record of the sonde. It is fully described in Sections 2.9.2 and 2.9.3 below. Details of any of the studies
can be obtained by pressing the number key associated with the file in the Directory.

Filename Samples

1-BRIDGE1.dat 19
2-BRIDGE2.dat 27
3-UPLAKE.dat 33
4-CLRLAKE2.dat 167
5-DWNLAKE2.dat 31
6-00003001.glp 3

Select option (0 for previous menu): 4

Select

2-Upload to view file lists in memory, and upload the data to PC-based software.

Prior to upload, a “
to upload. You may select

Time window” display appears that will allow you to select portions of the logged data

1-Proceed to upload all logged data from the dates and times displayed.

-----------------File-----------------

­1-Directory 4-View file
2-Upload 5-Quick view file
3-Quick Upload 6-Delete all files

Select option (0 for previous menu): 1

-------------File details--------

-----­1-View file
2-File:CLRLAKE2.dat
3-Samples: 167
4-Bytes: 4421
5-First:08/23/2001
6-First:08:33:40
7-Last :08/23/2001
8-Last :09:04:20
9-Interval:00:00:10
A-Site:Clear Lake

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--------------Time window-------------­1-Proceed
2-Start date=08/14/96
3-Start time=18:00:00
4-Stop date=08/28/96
5-Stop time=11:00:00

Select option (0 for previous menu):

Select

1-Proceed. Choose the appropriate file transfer protocol (in this example, PC6000) and a status box

will show the progress of the upload. Verification of a successful transfer is indicated when all of the
requested data have been transferred to the C:\ECOWIN\DATA subdirectory of your PC and automatically
assigned a “.DAT” extension.

---------------File type--------------­1-PC6000
2-Comma & ' ' Delimited
3-ASCII Text

Select option (0 for previous menu):

Select

3-Quick Upload to transfer the last logged file in its entirety to your computer. As noted above, the

file will then be resident in the C:\ECOWIN\DATA subdirectory.

4-View File to examine the data in any file currently stored in the sondes flash disk memory. You

Select
will first view the same screen as viewed in the Directory menu. From this menu choose the file of interest,
then, using the Time window menu, choose the dates and/or times of interest. If you choose dates or times
that are not within the designated start and stop times, no data will be displayed.

You also may choose the entire file. You can use the
time. Use the

Esc key to stop the view.

Space Bar to stop and restart the scrolling at any

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Select 5-Quick view file to view the last page of data from the last data file in flash disk memory. This
feature is particularly useful to quickly review any recently acquired data so that system performance can
be assessed.

6-Delete all files to IRREVERSIBLY remove all files (INCLUDING the .glp file that contains

Select
calibration information) from the sonde flash disk memory. It is critical not to use this option until all
relevant data from sonde memory is transferred to your computer via one of the upload options. There is a
verification screen that appears, so that pressing the number 6-key does not immediately delete all files.

REPORTING AND PLOTTING DATA WITH ECOWATCH

There are many features in EcoWatch related to viewing, plotting, manipulating and reporting data
collected from a 6-Series sonde. EcoWatch includes a Windows Help section for convenient reference that
describes all of the software’s features. This section of the manual describes the most commonly used
functions of the program and Section 4,
software.

For the purposes of describing and demonstrating EcoWatch plotting, reporting and data manipulation
capabilities, we use the file SAMPLE.DAT available on the compact disk that was enclosed with your 6­series sonde. This file will be copied to your hard drive during EcoWatch installation and should be
available to you for the instructions below.

When you are ready to move beyond the example SAMPLE.DAT file and analyze data collected from the
sonde, locate and open the appropriate .DAT file from your data upload exercise. These data are normally
found at C:\ECOWWIN\DATA\. An example filename is 1097CM01.DAT.

NOTE: In the instructions below that refer to clicking the mouse button, we always refer to the left mouse

button unless otherwise specified.

OPENING A DATA FILE

If EcoWatch is not running, open the program by double clicking on the EcoWatch icon. Click on
view a drop-down menu similar to the one shown in the figure below. From this menu click on
locate the drive and directory where SAMPLE.DAT (or your file of interest) resides. Alternatively, if you
have been using EcoWatch during setup and checkout, you may be able to click on the file of your choice
in the most recently opened files.

Once the SAMPLE.DAT file is open you should see a plot, as shown in the figure below, which graphically
represents seven days of sonde data for six different water quality parameters plotted as a function of date
and time. Each set of data is autoscaled to allow you to see the minimum and maximum values for each
parameter during the one-week study.

EcoWatch for Windows, is a detailed manual for EcoWatch

File to

Open, then

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Opening a File

Some daily variations may be noticed in parameters such as dissolved oxygen, pH and temperature in this
particular study. This is fairly typical in many natural bodies of water. Note also that conductivity is low
at both ends of the graph. You may notice similar perturbations in some of the other readings as well. In
this example, the sonde was not in the water for a short time at the beginning and end of the study. Not
only can you see exactly when the sonde went in and out of the water, but the bottom graph shows the
depth at which the sonde was deployed.

Notice also that you now see a new set of menu items in the top line. Some of these functions are
specifically related to viewing and manipulating data. Next we will examine some of the viewing options.

VIEWING DATA

To look at some of the viewing options, click on
Bar are turned on (check mark). In addition, the 4-Digit Year expression is checked. Also note that a
check mark is just left of the Graph choice. When a check is next to Graph, all data are expressed
graphically in the opening window.

To show data in both graphical and data table format, highlight the Table menu item, then click or press

Enter. The graphical portion of the window becomes compressed, and the data table becomes visible. If

you then click on
that both functions are turned on. You may use your mouse to scroll up/down and left/right to view data.

View again, both the Graph and Table items have check marks to their left, indicating

View (see figure below). Note that the Toolbar and Status

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Viewing Options

It may be somewhat awkward to scan the data table in this manner; therefore you have the option to turn
off the graphical representation and allow the table to fill the window. See the figure below. Notice now
that when you click on

Viewing the Data in Table Format

View, the Graph item is no longer checked.

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Viewing features such as Grid, Marker, Zoom In, Zoom Out and Unzoom are all available when you
activate the
EcoWatch.

Statistics and Study functions of EcoWatch are shown in the figure be low. Both provide overview

The
information related to the study data. The
deviation information for each parameter activated. The
the design of the study including sample interval, date/time, number of samples, sensor identification and
parameters reported. The Statistics and Study windows may be activated over either graph or table
presentations.

To view either of these windows, click on
window opens on top of the table or graph, similar to what is shown in the figure below. Only one of these
windows may open at one time. To continue, you must close the Statistics or Study window to return to the
graph or table and activate the top line menu again.

As before practice viewing the functions mentioned above to gain more familiarity with these features.

Graph function. Give each a try as you practice and learn more about the many features of

Statistics function lists minimum, maximum, mean and standard

Study function provides useful information about

View, highlight the desired function and click again. The

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Statistics and Study Information

Next, with the Statistics and Study windows closed, return to the

Graph.

Using the right mouse button, click at any point on the graph. A dotted vertical line appears along with
specific data values in boxes to the left of the displayed graphs, as shown in the figure below. You can
hold down the right mouse button and move the mouse to scan the entire graph that is displayed in the
window. The values in the boxes change as you move the mouse. This feature is very useful for
quantifying specific data without the need to open the data table and scroll through what may be thousands
of data points. Note also that the exact time and date change to let you know specifically when an event of
interest occurred.

View menu, close Table and activate

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Viewing the Data with Right-Button Mouse Function

CHANGING DISPLAY FORMATS USING SETUP

Beyond selecting data viewing options such as table format or graphical format, you may also customize
your data displays. For example, you may change the order in which parameters are viewed, you may add
and delete parameters, you may change plot appearance using different interval times and different units,
and you may change the x-axis if you prefer a parameter other than date or time.

The Top Line menu selection that allows you to select some of these parameter changes is

Setup, then Parameters. From here there are four submenus that allow you to Add/Remove parameters,

change Units, change sample interval and/or x-axis (Attributes) and change the Names of the parameters
you have assigned, as shown in the figure below.

Setup. Click on

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Changing the Appearance of a Graph or Table

If you are displaying the graph, you may change the appearance by changing font, font style, size and text
color. You may also change page color, trace color and graph background color. You may assign a custom
2-line title for the graph, and finally, you may display 1 trace or 2 per set of axes. For display of table
formatted results you may change font, font style, size and text color. In addition, you may change table
color and highlight color.

The menu structure is easy to follow. Try some changes to gain familiarity with these
options.

Setup display

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CHANGING DISPLAY FORMATS USING ‘GRAPH’ FUNCTION

The top line menu labeled
graphical format. You may be able to more clearly understand an event by zooming in/out, centering an
event of interest, and setting limits to focus in on a specific area of the graph. In addition to modifying
along the x-axis, you may also manually scale the y-axis. This may allow you to discard a noise spike and
obtain better resolution of events unrelated to the noise. Functions like Autoscale, Redraw and Cancel
Limits are all used to “undo” some of the customization functions. You will see some of these functions in
the figures below.

One very commonly used function is Limit Data Set. If you choose this function by clicking on the
highlighted item as shown below, you then use the mouse to move your cursor to the left limit of an area of
interest, click once, then move the mouse to the right limit of interest and click again. The result will be a
close up look at the specific area of the graph you have defined.

Selecting a Subset of Data within a Graph

Graph, as the name suggests, can be used to examine critical events within the

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Using Limit Data Set to Display a Subset of Data

To return to the full set of data again, click on
any graph or table, or even a subset expression as shown above, you may use the
‘copy’ the graph in the active window to the “Clipboard”. You can then ‘paste’ this graph to the Windows
application program of your choice. You may also be able to print graphs and tables as described in the
next section.

Graph, then Cancel Limits. If you desire a hard copy of

Edit, Copy command to

SAVE, IMPORT, EXPORT AND PRINT COMMANDS

File function in the top line menu, you can save a particular presentation version of a data file and

Under
give the presentation a customized name. For example, you might want to call the expanded plot in the
figure above “DIURNAL” using the File|Save Data Display command. This presentation will be saved
with your data file and can be recalled in further by using the
also export your custom data display as a spreadsheet compatible file (.CDF or .TXT) or print it to a
compatible printer. You may also create a custom report format using the File|Report command. See the
figure below for the
more about these features.

File menu, which shows these commands. Use the Window’s Help function to learn

File|Load Data Display command. You can

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Saving, Exporting, Printing and Related Functions

EXAMPLE OF CUSTOMIZING A SUBSET OF SAMPLE.DAT

To conclude this section we have used a few of the many tools available in EcoWatch to demonstrate how
you might use this powerful plotting and reporting program to express study results. We encourage you to
try some of the tools and learn more about EcoWatch by using the Window’s Help function, which is
available when the EcoWatch program is running.

Using SAMPLE.DAT we decided that some of the data were not of particular interest, so using top line
menu item
set. Note that we have not deleted this information from the file, but rather we are choosing not to display
it. You can always return to this function and add original data back. Under the same
function, we have selected
data was collected every 15 minutes, the change to a 60 minute interval helps to smooth out the graph and
average out any short term “noise” events.

Next, we again select
typed in a name (Clear Lake Study #2) and below that we typed the parameters that are shown in the graph.
Just below Title Page, we clicked on 2 Traces per Graph. This combines adjacent parameters which is
sometimes useful in parameter and event evaluation. For example, in the second graph shown in the figure
below, you see that DO concentration and pH seem to track rather closely and change in a diurnal rhythm.
In actuality, when DO levels drop in a natural body of water, CO
which leads to lower pH readings. DO rises again during th e day due to ph ot osynthesis, CO
pH increases again. The final plot after making these changes in shown in the figure below.

Setup, then Parameters, then Add/Remove, we removed ORP and Depth results from the data

Parameters

Attributes and changed the Average Interval from the default 0 to 60. Since

Setup, then Graph. From the functions available, we first selected Title Page… and

often builds up forming carbonic acid

2

then falls and

2

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Customizing a Graph from SAMPLE.DAT

Finally, we selected
presentation can be immediately recalled in the future.

As you become more familiar with EcoWatch for Windows, the plotting, analysis and reporting functions
can be accomplished easily and quickly. Practice with all of the functions and, again, do not forget to use
Window’s Help for more detail, or see Section 4,

File|Save Data Display and gave the custom plot the name “4PARAM” to that the

EcoWatch for Windows.

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2.9 SONDE MENU

The functions of the sondes are accessible through the sonde menu. The sonde menu structure makes it
simple and convenient to select functions. This section provides a description of the menus and their
capabilities.

When moving between menus within the sonde software structure, use the
previous menu. To exit menus and return to the sonde command line (the # sign), press 0 or Esc until the
question “
to the Main sonde menu, type
“menu” after the “#” and press

To save power, the sondes will power down automatically if no interaction from the keyboard occurs for
approximately 60 seconds when an Autosleep function (RS232 or SDI12) is activated in the Advanced

Setup menu. When the software is in this “sleep” mode, the first subsequent keystroke simply “wakes it

up” and has no visible effect on the display. The next keystroke after the unit is “awakened” will be input
to the software in the intended manner. Thus, if you press a key after the sonde has been inactive for some
time and nothing seems to happen, press the key again.

To gain experience with the sonde menus, select the sonde icon in EcoWatch for Windows and press

Enter. See the figure below for the Sonde Menu Flow Chart.

In the following subsections you will learn about the functions of the various menu items and when to use
them. The discussion of the menu and submenu functions is organized in numerical order, beginning with

Section 2.9.1 RUN.

0 or Esc to back up to the

Exit menu (Y/N)?” appears. Type Y and the command prompt (“#”) will be shown. To return

menu and press Enter. To return to the Main menu of the sonde, type

Enter.

IMPORTANT MESSAGE!

WHAT IF THERE IS NO RESPONSE TO A KEYSTROKE?

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2.9.1 RUN

Select 1-Run from the Main menu to begin taking readings or to set/verify the parameters required for a
study. There are two options in the Run menu.

---------------Run setup--------------­1-Discrete sample
2-Unattended sample

Select option (0 for previous menu): 1

DISCRETE SAMPLING

Discrete sampling is usually used in short term, spot sampling applications when the user is present at the
site and the unit is attached to a data logger or laptop PC. It could be used in the vertical profiling of a lake
or river where the user lowers the sonde incrementally into the water, and data acquired for a few minutes
at each depth. In this section, you will learn how to use the sonde menu structure to open and close files,
set the interval between samples, start the sampling operation, and log data to the internal memory of the
sonde.

1-Discrete sample from the Run menu. The Discrete sample menu will be displayed.

Select

------------Discrete sample-----------­1-Start sampling
2-Sample interval=4
3-File=
4-Site=
5-Open file

Select option (0 for previous menu):

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Select 1 – Start sampling option to start discrete sampling. After the initial sampling time interval has
passed (4 seconds in the screen above), sequential lines of data will appear on the screen.

=============================================================================

Date Time Temp SpCond Sal Cl2 Depth pH Turbid Battery

mm/dd/yy hh:mm:ss C mS/cm ppt mg/L feet NTU volts

-----------------------------------------------------------------------------­*** 1-LOG last sample 2-LOG ON/OFF, 3-Clean optics***
05/05/97 10:04:40 21.57 0.009 0.00 0.95 -0.293 7.55 0.3 10.2
05/05/97 10:04:44 21.57 0.009 0.00 0.96 -0.300 7.55 0.3 10.3
*** LOG is ON, hit 2 to turn it OFF, 3-Clean optics***
05/05/97 10:04:48 21.58 0.009 0.00 0.95 -0.302 7.55 0.3 10.3
05/05/97 10:04:52 21.58 0.009 0.00 0.95 -0.302 7.55 0.3 10.3
05/05/97 10:04:56 21.58 0.009 0.00 0.95 -0.303 7.55 0.3 10.2
05/05/97 10:05:00 21.58 0.009 0.00 0.96 -0.303 7.55 0.3 10.3
*** 1-LOG last sample 2-LOG ON/OFF , 3-Clean optics***
05/05/97 10:05:04 21.58 0.009 0.00 0.96 -0.304 7.55 0.3 10.3
Sample logged.
05/05/97 10:05:08 21.58 0.009 0.00 0.95 -0.305 7.55 0.3 10.3

The following prompt will appear just below the screen header:

1-LOG last sample, 2-LOG ON/OFF, 3-Clean optics

• By entering 1–LOG last sample, A single line of data can be logged to sonde memory and the

following message will be displayed:

• By entering

be displayed:

• By entering

probe, the wiper will clean the optical surface
appear if an optical probe was installed and enabled.

Esc or press 0 and return to the Discrete Sampling menu.

Select

2-LOG ON/OFF, a set of data can be logged to memory and the following message will

LOG is ON, hit 2 to turn it OFF, 3-Clean optics. Press 2 again to terminate logging.

3-Clean optics, if your unit has an optical (turbidity, chlorophyll or rhodamine WT)

Sample logged.

. The 3-Clean optics portion of the prompt will only

From the Discrete Sampling menu, select 2-Sample Interval to type a number that represents the number

seconds between samples. The maximum sample interval is 32767 seconds (9+ hours). The factory

of
default sample interval is 4 seconds and works best for most discrete sampling applications. The shortest
possible sample interval for obtaining new free chlorine readings is 4 seconds. If a smaller interval is
selected, then the Cl2 readings remain constant for the number of samples necessary to fill 4 seconds.

NOTE: If your have used your sonde with a 650 MDS data logger, the Sample In terval automatically be

changed to 0.5 seconds.

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Sampling Faster Than 0.5 Seconds

For special applications, your sonde is capable of faster sampling. The only limitation is a reduction of the
number of sensors selected. To determine the maximum sampling frequency for your sensor setup, divide
36 by the number of enabled sensors in addition to the free chlorine sensor.

Example:

If you enable any three sensors plus DO, divide 36 by 3 to obtain 12 samples/second (12 Hz) or 0.083
seconds between samples as the maximum sampling frequen cy. Remember that for sample intervals less
than 4 seconds, free chlorine readings are updated only every 4 seconds. Thus, at 12 Hz, the display will
show 48 identical Cl2 readings before a change occurs. Note also that at a faster sampling rate, consecutive
readings are closer together, the threshold for any one sensor is less likely to be exceeded, and the data
filter is less likely to disengage. For these applications you may want to adjust your threshold settings
accordingly. See Section 2.9.8 for more information on the data filter.

Select 3-File to enter a filename with a maximum of 8 characters. You will log readings to the filename
that you enter.

If you started sampling without entering a filename, the default name NONAME1 will be assigned to your
file. Whenever you press
opened during sampling. If this happens, and you want to restart the file with a different name, press

Close file

Select
the site where you are sampling.

When you select
finished logging data to the file, press
start logging the

Now select 1-Start sampling to start discrete sampling.

After the initial sampling time interval has passed, sequential lines of data will appear on the screen and
you can log single or sequential data points and wipe the surface of your optical probe as described above.

and rename the file.

4-Site to assign a site name with a maximum of 31 characters This allows you to enter the name of

5-Open File, a file is opened and the number 5 changes to Close File. When you are

Open File designation changes automatically to Close File.

1-LOG last sample or 2-LOG ON/OFF from the menu, NONAME1 will be

5-

5-Close File and designation changes back to Open File. When you

UNATTENDED SAMPLING

This option is used for long-term deployment of the battery-powered sonde when the user is not present at
the site. Prior to this selection, the sonde is usually attached to a computer in a laboratory and set up to
automatically log data to sonde memory at a fairly long time interval (15-60 minutes). The unit is then
taken to the field site and left for an extended time period (30 – 60 days). When the study is complete, or
the batteries are expended, the unit is brought back to the laboratory and the data file is uplo aded to a
computer. Quality assurance checks are performed prior to redeployment.

Select

2-Unattended Sampling from the Run menu. The Unattended sample menu will be displayed. Use

the following example to understand the unattended sampli ng option.

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------------Unattended setup----------­1-Interval=00:15:00
2-Start date=07/17/96
3-Start time=18:00:00
4-Duration days=14
5-File=clrlake3
6-Site=Clear Lake at Spillway
7-Bat volts: 9.1
8-Bat life 21.2 days
9-Free mem 18.9 days
A-1st sample in 8.10 minutes
B-View params to log
C-Start logging

Follow the prompts on this screen to prepare your 600XLM, 6600, 6920, or 600 OMS (battery version)
sonde for unattended deployment as described below:

• Verify that the current time and date are correct to ensure that your unattended sampling study begins

and ends when you desire. To verify correct the time and date, return to the Main menu and enter

or 5-System. You may enter the correct date and time from either of these submenus.

Status

• Select

• Select

It is better to start the study prior to taking the unit to the field so that you can confirm that readings are
being saved to memory and carry out initial quality assurance measurements. However, if your study
demands that you start the instrument at the site at 6:00 PM, change the Start Time to 18:00:00.

1-Interval and enter the desired time between samples. Use the 24-hour clock format to enter

interval.

2-Start Date and 3-Start Time to set the time that data will begin to log to sonde memory. If

you do not make any change to these entries, then the study will automatically begin at the next
integral time interval once you have pressed

Example: If the current time is 17:20:00 and your sample interval is 15 minutes, logging will

automatically begin at 17:30:00.

C-Start logging.

4-

• Select 4-Duration and set the length of the study in days. The default value is 365 days (which is

longer than most deployments). In most cases, you will either want to stop the unattended study
manually or allow the batteries to be expended. It is wise to set the duration to a value longer than the
anticipated deployment. If you cannot retrieve the sonde at the expected time due to factors beyond
your control such as weather or illness, data will continue to be acquired as long as battery power is
present.

• Select

• Select

• Check

5-File and enter a name of no more than 8 characters that will be used by your external

computer to identify the study. Be sure to use ONLY alpha/numeric characters.

6-Site and enter a site name of no more than 31 characters. This filename will appear in your

sonde file directory, but will not be used to identify the file after transfer to your computer.

7-Battery to make certain that the voltage is suitable for the length of the study that you are

about to begin. No change can be made to this item via the software.

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• Select B-View Parameters to log to confirm that your sensor and report setups are configured

correctly as described in Sections 2.9.6 and 2.9.7. An example screen is shown below.

-------------Params to log------------­1-Temp C 6-Orp mV
2-Cond mS/cm 7-NH4+ N mg/L
3-Cl2 mg/L 8-NO3- N mg/L
4-Cl2chrg 9-Turbid NTU
5-pH A-Battery volts

Select option (0 for previous menu):

In some cases, the
calculation of the items that you have selected in the Report setup.

Example: f specific conductance is selected in the Report setup, it does not appear under
Parameters to log because it is calculated from Conductivity and Temperature. In all but a few

cases, the proper configuration of
sensor is enabled.

There are several items that must be activated in the Report setup so they will be available from the file
after upload. These special parameters are: Cl2 Chg, pH mV, NH4
Fluorescence %FS. If you want to log any of these parameters to your data file, be certain that they are
active in the Report setup before you begin the unattended study.

After making the above entries, the sonde software will automatically estimate the expected battery life,
and the time it will take for the sonde memory to be filled. This information is displayed on the screen for
your consideration as items 9 and A. If the battery life or the free memory capacity will be exceeded
sooner than the duration, you may want to make some changes to th e entries. For example, you can free up
memory in the sonde by uploading all existing files from the sonde memory to your PC and then deleting
them from the sonde (see
battery life. You can lengthen the sampling interval to extend both battery life and memory capacity.

View params to log screen only identifies the raw parameters that are used in the

Parameters to log will be automatically set up as long as the

+

mV, NO3- mV, Cl -mV, and

3-File from Main Menu). You may want to change the batteries for longer

The predicted battery life is an estimate only. The temperature of the site and the brand of batteries used
can affect battery life. It is recommended to recover the sonde earlier than the predicted battery life, and to
use new batteries for each deployment.

Once you press

C-Start logging, the following screen will appear.

-------------Start logging------------­Are you sure?
1-Yes
2-No

Select option (0 for previous menu):

Select

1-Yes and the screen will change.

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----------------Logging---------------­1-Interval=00:15:00
2-Next at 07/17/96
3-Next at 18:00:00
4-Stop at 07/31/96
5-Stop at 18:00:00
6-File=clrlake3
7-Site=Clear Lake at Spillway
8-Bat volts: 9.0
9-Bat life 21.2 days
A-Free mem 18.9 days
B-Stop logging

Select option (0 for previous menu):

The display now shows the next date and time for logging and the stop date and time for the logging study.
Most importantly, note that the bottom command now shows
logging has indeed been initiated.

The Unattended study will terminate when the duration you specified has expired or the batteries are
expended. If you want to terminate sooner, simply select

B-Stop logging. Select 1-Yes and return to the Unattended setup menu.

B-Stop logging, a confirmation that the

2-Unattended sample from the Run menu, then

Stop logging?
1-Yes
2-No

Select option (0 for previous menu):

2.9.2 CALIBRATE

All of the sonde sensors (except temperature) require periodic calibration to assure high performance.
However, the calibration protocols for free chlorine are significantly different depending on whether the
sonde is being set up for spot sampling or longer term unattended monitoring studies. This difference is
user-selectable and is required primarily because the optimal performance of the free chlorine sensor
cannot be attained unless the control of this sensor varies from short term to long term applications.

For spot sampling it is best to pulse the sensor continuously during the Run mode to attain the most
accurate results and optimize the response time. However, this continuous pulsing is not ideal for longer
term logging studies in which the sonde data is captured to sonde memory or to a data collection platform
at much less frequent intervals (e.g. 15 minutes). Continuous pulsing not only shortens the time between
required probe maintenance, but consumes more power. With proper selection of the “Auto sleep” option
(discussed in detail in
run continuously or “go to sleep” between samples to minimize free chlorine probe wear and conserve
power. The effect of this choice on the user interface relative to free chlorine calibration is significant as
described below:

Section 2.9.8, Advanced Menu), the user can configure the sonde software to either

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Sondes Section 2

 If “Auto sleep” is deactivated, the sonde runs continuously no matter what sample interval has been

selected. Under these conditions, the user retains manual control of the free chlorine calibration
routine, viewing the stabilization of the readings in real time and confirming the calibration with
keyboard entries.

 If “Auto sleep” is activated, the sonde will ‘warm up’ the sensors for the period of time selected for

the free chlorine sensor. Under these conditions, the user loses manual control of the free chlorine
calibration routine. Free chlorine will
Cl2 sensor has expired. In this mode of calibration, you do not observe stabilization of the readings in
real time, but instead will observe a countdown of the warm up period followed by a message
indicating that the free chlorine calibration is complete.

automatically calibrate after the selected time for warm up of the

Only the calibration of free chlorine is affected by whether “Auto sleep” is on or off; the user retains
manual control of the calibration of all other parameters regardless of the “Auto sleep” setting. Once a
particular warm up time (in seconds) has been utilized in free chlorine calibration, the length of that time
should not be changed during a study. A new calibration should be performed whenever the value of the
warm up time is altered.

From the Main sonde menu select 2-Calibrate. The Calibrate menu will be displayed. Only the enabled
parameters will be available for calibration.

---------------Calibrate-------------­1-Conductivity 6-ISE3 NH4+
2-Free Cl2 7-ISE4 NO3­3-Pressure-Abs 8-ISE5 Cl­4-ISE1 pH 9-Turbidity
5-ISE2 ORP A-Chlorophyll

Select option (0 for previous menu):

CONDUCTIVITY

Select 1-Conductivity to calibrate the conductivity probe and a second menu will offer you the options of
calibrating in specific conductance, conductivity, or salinity. Calibrating any one option automatically
calibrates the other two. After selecting the option of choice (specific conductance is normally
recommended), you will be asked to enter the value of the standard used during calibration. Be certain that
the units are correct. After pressing
confirm the calibration when the readings are stable by pressing
as instructed, press

Enter again to return to the Calibrate menu.

Enter, you will be able to follow the stabilization of the readings and

Enter as instructed on the screen. Then,

FREE CHLORINE WITH FOR UNATTENDED STUDIES WITH INTERNAL LOGGING OF DATA

NOTE CAREFULLY: The 6572 free chlorine sensor MUST be calibrated in situ, i.e. in flowing drinking

water whose free chlorine content has been determined by an independent method. In many cases, the
independent method involves the use of the DPD Colorimetric Method as defined in Section 4500-Cl G of
Standard Methods for the Examination of Water and Wastewater. Relatively inexpensive colorimeters,
such as the YSI Models 9000 and 9100 and the Hach Model 5870000, are available for carrying out the
DPD method to determine the free chlorine in the effluent of the flow cell associated with the 6920DW or
600DW-B drinking water studies.

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In most cases, the free chlorine sensor should be calibrated AFTER a logging study has been underway for
2-3 hours to assure the stabilization of the free chlorine sensor in the flowing tap water. The setup of the
logging study (or Unattended Sampling) is described in detail in

SAMPLING

prior to calibration, see

To calibrate the free chlorine sensor carry out the following steps:

Following the calibration of all other sensors present, reinstall the free chlorine probe in the sonde bulkhead
after removing the port plug. Make certain that no moisture is present in the port before installing the
probe and, if moisture is present from the previous calibration procedures, use compressed air to dry the
port.

• Break in the sensor according to the instructions in Section 2.6 above.

• Locate the flow cell which was supplied with your 6920DW or 600DW-B and attach it to the sonde

according to the instructions that were supplied with the flow cell. Note that for flow cell studies on
drinking water with the 600DW-B, YSI recommends that users purchase a 655488 Extended
Calibration Cup Sleeve and substitute the extended length sleeve from the calibration cup for the
shorter sleeve in the flow cell kit. The extra length will eliminate any bottom interference on the
turbidity readings and will be compatible with calibration of the turbidity sensor using the extended
length calibration cup.

• Attach inlet and outlet hoses to the barbed fittings supplied with the flow cell and then insert the

fittings into the proper ports of the flow cell. Make certain that the hoses are attached very snugly to
the barb fittings to prevent any possibility of catastrophic leakage. If necessary, standard hose clamps
can be used to secure the hoses to the barbed fittings.

• Connect the inlet line of the flow cell to a drinking water tap and run the outlet line to a drain.

• Turn on the water to the cell so that the flow is 500-1000 mL per minute (minimum 500 mL/min)

• Set up your Unattended Sampling study as described above and allow readings to be logged to

• Collect a sample of water from the outlet hose of the flow cell and immediately analyze it for free

• Interface to the sonde using a 650 display/logger or a PC running EcoWatch for Windows.

and 2.9.1 Run UNATTENDED SAMPLING. To view the logged data to assure stability

Section 2.9.3 File.

Make certain that the inlet line is securely attached to the tap and that the outline line will not pop
out of the drain under flow conditions.

and allow water to flow through the cell for at least 1 hour before proceeding.

internal memory for 2-3 hours. Using the
inspect the free chlorine readings and after they are stable proceed with the calibration as
described below.

chlorine using an independent method , e.g. , t he DP D method with a YSI 9100 Photometer.

Choose
shown in the box below.

8-Advanced and then 2-Setup and make certain that Autosleep RS-232 is active as

File|View command as described in Section 2.9.3,

Section 2.7 UNATTENDED

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-------------Advanced setup----------­1-(*)VT100 emulation
2-( )Power up to Menu
3-( )Power up to Run
4-( )Comma radix
5-(*)Auto sleep RS232
6-(*)Auto sleep SDI12
7-( )Multi SDI12
8-( )Full SDI12

Select option (0 for previous menu): 0

Return to the Main menu using the
At the prompt, input the independently determined free chlorine value and press Enter. A display similar

to that shown below will appear on either the computer monitor or the 650 display. The calibration will
occur automatically after the countdown on the screen reaches zero.

Esc key and select 2-Calibrate and then 4-Free Cl2.

===================================================================
Temp SpCond Sal Cl2 Cl2 Chrg Depth pH NH4+ N NO3- N Turbid
C mS/cm ppt mg/L feet mg/L mg/L NTU

------------------------------------------------------------------­Stabilizing: 38

After the warm-up time is complete, the readings just before and just after calibration are displayed. When
you press

Enter, the screen returns to the Free Chlorine Calibration menu.

FREE CHLORINE WITH SONDE ATTACHED TO 6500 PROCESS MONITOR

See Appendix G. Using Your Drinking Water Sonde with a YSI

6500 Process Monitor

Hold feature.

for enhanced calibration instructions and a detailed description of the Sample and

NOTE CAREFULLY: The 6572 free chlorine sensor MUST be calibrated in situ, i.e. in flowing drinking

water whose free chlorine content has been determined by an independent method. In many cases, the
independent method involves the use of the DPD Colorimetric Method as defined in Section 4500-Cl G of
Standard Methods for the Examination of Water and Wastewater. Relatively inexpensive colorimeters,
such as the YSI Models 9000 and 9100 and the Hach Model 5870000, are available for carrying out the
DPD method to determine the free chlorine in the effluent of the flow cell associated with the 6920DW or
600DW-B drinking water studies.

In most cases, the free chlorine sensor should be calibrated AFTER it has been attached to the 6500 Process
Monitor for 2-3 hours to assure the stabilization of the free chlorine sensor in the flowing tap water. See

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the manual which was supplied with your 6500 for the setup of the overall system and a de scription of the
displayed information.

To calibrate the free chlorine sensor using a 6500, carry out the following steps:

• Following the calibration of all other sensors present, reinstall the free chlorine probe in the sonde

bulkhead after removing the port plug. Make certain that no moisture is present in the port before
installing the probe and, if moisture is present from the previous calibration procedures, use
compressed air to dry the port.

• Break in the sensor according to the instructions in Section 2.6 above.

• Locate the flow cell which was supplied with your 6920DW or 600DW-B and attach it to the sonde

according to the instructions that were supplied with the flow cell. Note that for flow cell studies on
drinking water with the 600DW-B, YSI recommends that users purchase a 655488 Extended
Calibration Cup Sleeve and substitute the extended length sleeve from the calibration cup for the
shorter sleeve in the flow cell kit. The extra length will eliminate any bottom interference on the
turbidity readings and will be compatible with calibration of the turbidity sensor using the extended
length calibration cup.

• Attach inlet and outlet hoses to the barbed fittings supplied with the flow cell and then insert the

fittings into the proper ports of the flow cell. Make certain that the hoses are attached very snugly to
the barb fittings to prevent any possibility of catastrophic leakage. If necessary, standard hose clamps
can be used to secure the hoses to the barbed fittings.

• Connect the inlet line of the flow cell to a drinking water tap and run the outlet line to a drain.

Make certain that the inlet line is securely attached to the tap and that the outline line will not pop
out of the drain under flow conditions.

• Turn on the water to the cell so that the flow is 500-1000 mL per minute (minimum 500 mL/min)

and allow water to flow through the cell for at least 1 hour before proceeding.

• Connect the drinking water sonde to the 6500 using a YSI 609X field cable. Make sure that

readings are displayed on the screen in real-time.

• Activate the Sample and Hold feature of the software by pressing the

6500 front panel simultaneously and then selecting the
the feature, use the arrow keys to set the sample interval.

• Observe the displayed free chlorine readings for 2-3 hours to assure that they are stable. Then

proceed with the calibration as described below.

• Collect a sample of water from the outlet hose of the flow cell and immediately analyze it for free

chlorine using an independent method , e.g. , t he DP D method with a YSI 9100 Photometer.

• Press the “Cal” key on the 6500 face plate and then select Free Cl2. Press

the correct chlorine value in mg/L as determined by the DPD test. Press
will occur automatically. See the 6500 manual for additional details about the calibratio n
procedure.

Setup sonde menu. After activation of

Esc and Enter keys on the

Enter and then input

Enter and the calibration

pH

When selecting 4–ISE1 pH, you will be given the choice of 1-point, 2-point, or 3-point calibrations.

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Select the

1-point option only if you are adjusting a previous calibration. If a 2-point or 3-point calibration

has been performed previously, you can adjust the calibration by carrying out a one point calibration.
Immerse the sonde in a buffer of known pH value and press
value of the solution. Press

Enter again, and the screen will display real-time readings that will allow you

to determine when the pH and temperature readings have stabilized. Pressing
calibration. Then, as instructed, press

Enter again to return to the Calibrate menu. This calibration

Enter. You will be prompted to type in the pH

Enter will confirm the

procedure adjusts only the pH offset and leaves the previously determined slope unaltered.

Select the

2-point option to calibrate the pH probe using only two calibration standards. In this procedure,

the pH sensor is calibrated using a pH 7 buffer and pH 4 buffer. A two point calibration procedure (as
opposed to a 3-point procedure) can save time if the pH of the media being monitored is known to be either
basic or acidic. For example, if the pH of a pond is known to vary between 5.5 and 7, a two-point
calibration with pH 7 and pH 4 buffers is appropriate. Three point calibration with an additional pH 10
buffer will not increase the accuracy of this measurement since the pH is not within this higher range.

To begin the calibration, immerse the sonde in one of the buffers and enter the actual pH value. Press

Enter, and the screen will display real-time readings that will allow you to determine when the pH sensor

has stabilized. Pressing
the sonde in the second pH buffer, input the pH value, press
on the screen in real time. After the readings have stabilized, press
as instructed, press

Enter will confirm the calibration. Following the instructions on the screen, place

Enter, and view the stabilization of the values

Enter to confirm the calibration. Then,

Enter again to return to the Calibrate menu.

Select the

3-point option to calibrate the pH probe using three calibration solutions. In this procedure, the

pH sensor is calibrated with a pH 7 buffer and two additional buffers. The 3-point calibration method
assures maximum accuracy when the pH of the media to be monitored cannot be anticipated. The
procedure for this calibration is the same as for a 2-point calibration, but the software will prompt you to
select a third pH buffer to complete the 3-point procedure.

ORP

Select 5-ISE2 ORP to calibrate the ORP sensor. Immerse the sonde in a solution with a known oxidation
reduction potential value (we recommend Zobell solution) and press
the ORP value of the solution. Press

Enter, and monitor the stabilization of the ORP and temperature

readings. After no changes occur for approximately 30 seconds, press
Then, as instructed, press

Enter again to return to the Calibrate menu.

Enter. You will be prompted to enter

Enter to confirm the calibration.

The following calibrations are for the 6920DW sonde only. If you do not have this sonde, skip to
Section 2.9.6, Report.

AMMONIUM

When selecting 6–ISE3-NH4+, you will be given the choice of 1-point, 2-point, or 3-point calibrations for
your ammonium (NH

Select the 1-point option only if you are adjusting a previous calibration. If a 2-point or 3-point calibration
has been performed previously, you can adjust the calibration by doing a one point calibration. Immerse
the sonde in any solution of known ammonium concentration and press
type in the NH

4

will display real-time readings that will allow you to determine when the NH4
Pressing

Enter will confirm the calibration.

Select the

2-point option to calibrate the NH

approximately the temperature of your environmental sample. In this procedure, the NH

+

) sensor.

4

+

value (in mg/L of NH4-N) of the solution you are using. Press Enter again, and the screen

+

probe using only two calibration standards that are both at

4

Enter. You will be prompted to

+

readings have stabilized.

+

sensor is usually

4

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calibrated using solutions that contain 1 and 100 mg/L of NH4-N . Be certain that the calibration solution
and sensor are thermally equilibrated prior to entering NH

+

values.

4

To begin the calibration immerse the sonde in the 1 mg/L standard, press
and again press
the sensor has stabilized. Pressing
the screen, place the sonde in the second NH
again press
have stabilized, press

Enter. The screen will display real-time readings that will allow you to determine when

Enter will confirm the first calibration. Following the instructions on

+

standard, press Enter, input the correct concentration value,

4

Enter, and view the stabilization of the values on the screen in real time. After the readings

Enter to confirm the calibration. Then, as instructed, press any key to return to the

Enter, input the NH

-N value,

4

Calibrate menu.

Select the

3-point option to calibrate the NH

+

probe using three calibration solutions, two at ambient

4

temperature and one at a temperature significantly different from ambient. The 3-point calibration method
should be used to assure maximum accuracy when the temperature of the media to be monitored cannot be
anticipated. The procedure for this calibration is the same as for a 2-point calibration, but the software will
prompt you to place the sonde in the additional solution to complete the 3-point procedure. Be certain that
the calibration solution and sensor are thermally equilibrated prior to proceeding with the calibration. The
recommended order of calibration standards is (1) 100 mg/L standard at ambient temperature, (2) 1 mg/L
standard at ambient temperature, and (3) 1 mg/L standard at a different temperature (usually lower) than
ambient. For best results, insure a temperature difference of at least 10 C° between the two 1 mg/L
standards.

NOTE: YSI strongly recommends the use of the 3-point protocol to ensure the best possible performance

from all ISE sensors (ammonium, nitrate, and chloride)

NITRATE

When selecting 7–ISE4 NO3, you will be given the choice of 1-point, 2-point, or 3-point calibrations for
your nitrate (NO
calibrant values are in mg/L of NO

NOTE: YSI strongly recommends the use of the 3-point protocol to ensure the best possible performance

from all ISE sensors (ammonium, nitrate, and chloride)

CHLORIDE

-

) sensor. The procedure is identical to that for the ammonium sensor, except that the

3

-N instead of NH4-N.

3

When selecting 8–ISE5-CL-, you will be given the choice of 1-point, 2-point, or 3-point calibrations for
your chloride (Cl
calibrant values are in mg/L of Cl instead of NH
standards for chloride that are 10 times greater than for ammonium and nitrate. Thus, the low calibration
value should be 10 mg/L and the high calibration value should be 1000 mg/L Cl-. The difference is due to
the fact that the effect of contamination of standards from inadvertent leakage of chloride ion from either
the free chlorine probe or the reference junction of the pH probe will be less significant at higher
concentrations.

NOTE: YSI strongly recommends the use of the 3-point protocol to ensure the best possible performance

from all ISE sensors (ammonium, nitrate, and chloride)

OPTIC TURBIDITY

-

) sensor. The procedure is identical to that for the ammonium sensor, except that the

-N. IMPORTANT: We recommend that the user employ

4

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NOTE: Before calibrating your 6026 or 6136 turbidity sensor, pay particular attention to the following

cautions:

• To properly calibrate YSI turbidity sensors, you MUST use standards that have been prepared

according to details in Standard Methods for the Treatment of Water and Wastewater (Section 2130 B).
Acceptable standards include (a) formazin prepared according to Standard Methods; (b) dilutions of
4000 NTU formazin concentrate purchased from Hach; (c) Hach StablCal
denominations; and (d) AMCO-AEPA standard s prepared specifically for the 6026 and 6136 by either
YSI or approved vendors who are listed on the YSI website (www.ysi.com). STANDARDS FROM
OTHER VENDORS ARE
USE WILL LIKELY RESULT IN BOTH CALIBRATION ERRORS AND INCORRECT FIELD
READINGS.

For AMCO-AEPA standards, the value entered by the user during the calibration protocol is

•

DIFFERENT depending on which sensor (6026 or 6136) is being calibrated. This reflects the
empirically determined fact that 6026 and 6136 sensors that have been calibrated to the same value in
the primary standard formazin, will have
beads. This effect is likely due to the larger optical cell volume of the 6136. Thus, for example, the
label of the YSI 6073 turbidity standard bottle indicates that the value of the standard is

when used for calibration of the 6026 sensor, but 123 NTU when used to calibrate the 6136.

Note that the phenomenon of a sensor-specific formazin/AEPA-AMCO ratio is well known for sensors
other than the 6026 and 6136.

• The details below are relevant to the calibration of both turbidity sensors offered by YSI – 6026 and

6136. However, to calibrate a 6136 sensor, you should have Version 2.16 or later installed in your
sonde.

NOT APPROVED FOR THE YSI TURBIDITY SYSTEM AND THEIR

different responses in suspensions of the AEPA-AMCO

TM

standards in various NTU

100 NTU

• If you are calibrating a 6136 turbidity sensor you MUST use either a black bottomed calibration cup

arrangement or laboratory glassware with the probe guard installed for the calibration. See section 2.6.1
above for details. Failure to carry out the calibration properly can result in inaccurate readings,
particularly water of very low turbidity.

• Before proceeding with the calibration, be certain that the probe compartment of the sonde has been

cleaned and is free of debris. Solid particles from this source, particularly those carried over from past
deployments, will contaminate the standards during your calibration protocol and cause either
calibration errors and/or inaccurate field data.

When selecting Optic T (or OpticC)-6026-Turbidity (or 6136-Turbidity), there will be a choice of 1­point, 2-point, or 3-point calibrations for your turbidity sensor.

1-point option is normally used to zero the turbidity probe in 0 NTU standard. Place the sonde in clear

The
water (deionized or distilled) with no suspended solids, and input 0 NTU at the screen prompt. Press
and the screen will display real-time readings that will allow you to determine when the turbidity readings
have stabilized. Press Enter after the readings have stabilized to confirm the calibration and zero the
sensor. Then, as instructed, press any key to return to the Calibrate menu.

Select the
one of the standards
for the water to be monitored. For example, if the water to be evaluated is known to be low in turbidity, an
appropriate choice of standards might be 0 and 10 NTU. However, for ge neral purpose measurements an
appropriate choice of standards is usually 0 and 100 NTU.

To begin the calibration, immerse the sonde in the 0 NTU standard, as instructed, and press
mandatory that the 0 NTU standard be calibrated first. The screen will display real-time readings that will
allow you to determine when the readings have stabilized. Pressing

2-point option to calibrate the turbidity probe using only two calibration standards. In this case,

must be clear water (0 NTU) and the other should be in the rang e of kno wn turbidity

Enter. It is

Enter will confirm the first calibration.

Enter

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Following the instructions on the screen, place the sonde in the second turbidity standard, input the correct
turbidity value in NTU, press
After the readings have stabilized, press
key to return to the Calibrate

Select the
point procedure, one of the standards
sensors, we recommend that the other two standards have turbidity values of 100 and 1000 NTU.
However, the user can select any values that are deemed appropriate. The procedure for this calibration is
the same as for a 2-point calibration, but the software will prompt you to place the sonde in the additional
solution to complete the 3-point procedure.

For all turbidity calibration procedures, be certain that the standard and sensor are thermally equilibrated
prior to proceeding with the calibration.

For further information related to setting up, calibrating and running turbidity measurements, refer to

Section 5.11.

3-point option for maximum accuracy over the entire range of 0 to 1000 NTU. As for the 2-

Enter, and view the stabilization of the values on the screen in real-time.

Enter to confirm the calibration. Then, as instructed, press any

menu.

must be 0 NTU. Because of the linearity characteristics of the

OPTIC CHLOROPHYLL

When selecting
fluorescence sensor (

or 3-point

If you have selected
of fluorescence in the sample being measured. These values could then be converted into actual chlorophyll
concentrations in µg/L by using a post-calibration procedure, after the chlorophyll content of grab-samples
taken during a sampling or monitoring study has been analyzed in a laboratory. This determination can
involve conducting the extractive analysis procedure described for chlorophyll in Methods for the
Examination of Water and Wastewater or by carrying out an in situ measurement of chlorophyll using a
commercial benchtop fluorometer.

Fluor zero option is used to zero the fluorescence probe in a medium that is chlorophyll-free. Place

The
the sonde in clear water, and input 0 at the screen prompt. Press Enter and the screen will display real­time readings that will allow you to determine when the fluorescence readings have stabilized. Press
after the readings have stabilized to confirm the calibration and zero the sensor. Then, press any key to
return to the Calibrate menu.

If you select
options. The 1-point selection is normally used to zero the fluorescence probe in a medium that is
chlorophyll-free. If you use this method, you will either choose to utilize the default sensitivity for
chlorophyll in the sonde software or to update a previous multipoint calibration. Usually you will place the
sonde in clear water, and input 0 µg/L at the screen prompt. After pressing
real-time readings allowing you to determine when the chlorophyll readings have stabilized. Press
after the readings have stabilized to confirm the calibration and zero the sensor. Then, as instructed, press
any key to return to the Calibrate menu.

Note: For the 2-point and 3-point calibrations described below, standards of known fluorescence are

required. Two general types of standards can be used: (a) phyto pl an kt o n sus pensions of known chlorophyll
content, and (b) dye solutions whose fluorescence can be correlated to that of chlorophyll. The user is
responsible for determining the chlorophyll content of phytoplankton suspensions, either by employing the
extractive analysis procedure described in Standard Methods for the Examination of Water and
Wastewater, or by analyzing the suspension in situ using a laboratory fluorometer. See Section 5,

Principles of Operation of this manual for more information about chlorophyll standards.

10-Optic Chlorophyll, there will be a choice of zeroing the full scale range of the

Fluor Zero), or calibrating with actual chlorophyll standards (µg/L 1-point, 2-point,

).

Fluor %FS as a parameter in Report Setup, the sonde will report only relative values

Enter

Chl µg/L in the initial calibration routine, there will be a choice of 1-point, 2-point, or 3-point

Enter the screen will display

Enter

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Select the 2-point option to calibrate the chlorophyll probe using only two calibration standards. In this
case, one of the standards must be clear water (0 µg/L) and the other should be in the range of a known
chlorophyll content of the water to be monitored. For example, if the water to be evaluated is known to be
low in chlorophyll, an appropriate choice of standards might be 0 and 10 µg/L. However, for general­purpose measurements an appropriate choice of standards is usually approximately 100 µg/L.

To begin the calibration, immerse the sonde in the 0 µg/L standard, as instructed, and press
mandatory that the 0µg/L standard be calibrated first. The screen will display real-time readings that will
allow you to determine when the readings have stabilized. Pressing
Following the instructions on the screen, place the sonde in the second chlorophyll standard, input the
correct value in µg/L, press
the readings have stabilized, press
return to the Calibrate menu.

Select the
point procedure, one of the standards must be 0 µg/L. The user can select any values for the second and
third standards that are deemed appropriate. The procedure for this calibration is the same as for a 2-point
calibration, but the software will prompt you to place the sonde in the additional solution to complete the 3­point procedure.

For all chlorophyll calibration procedures, be certain that the standard and sensor are thermally equilibrated
prior to proceeding with the calibration

CAUTION: To be assured of the accuracy for field measurements made with your chlorophyll sensor, you

must either initially calibrate the sensor using a phytoplankton suspension of known chlorophyll content or
post calibrate you sensor in a spreadsheet by comparing the fluorescence values obtained in field studies
with those obtained by laboratory analysis for chlorophyll on grab samples collected during the field study.
The use of chemical dye standards for “calibration” of the sensor may not result in accurate correlation
between fluorescence and actual chlorophyll readings and is designed primarily to evaluate the sensor for
drift. See Section 5. Principles of Operation for more information on the proper calibration of your
chlorophyll sensor.

3-point option for maximum accuracy over the entire range of 0 to 400 µg/L. As with the 2-

Enter, and view the stabilization of the values on the screen in real-time. After

Enter to confirm the calibration. Then, as instructed, press any key to

Enter will confirm the first calibration.

Enter. It is

OPTIC RHODAMINE WT

When selecting Rhodamine, there will be a choice of 1-point, 2-point, or 3-point calibrations for your
rhodamine WT sensor.

1-point option is normally used to zero the rhodamine probe in 0 ug/L standard. Place the sonde in

The
clear water with no suspended solids, and input 0 ug/L at the screen prompt. Press
will display real-time readings that will allow you to determine when the rhodamine readings have
stabilized. Press Enter after the readings have stabilized to confirm the calibration and zero the sensor.
Then, as instructed, press any key to return to the Calibrate menu.

Select the
case, one of the standards
rhodamine concentration projected for the study. For general purpose measurements an appropriate choice
of standards is usually 0 and 100 ug/L.

To begin the calibration, immerse the sonde in the 0 ug/L standard, as instructed, and press
mandatory that the 0ug/L standard be calibrated first. The screen will display real-time readings that will
allow you to determine when the readings have stabilized. Pressing Enter will confirm the first calibration.
Following the instructions on the screen, place the sonde in the second rhodamine standard, input the
correct rhodamine value in ug/L, press

2-point option to calibrate the rhodamine probe using only two calibration standards. In this

must be clear water (0 ug/L) and the other should be in the range of the predicted

Enter, and view the stabilization of the values on the screen in real-

Enter and the screen

Enter. It is

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time. After the readings have stabilized, press Enter to confirm the calibration. Then, as instructed, press
any key to return to the Calibrate

Select the
procedure, one of the standards
point calibration, but the software will prompt you to place the sonde in the additional solution to complete
the 3-point procedure.

For all rhodamine calibration procedures, be certain that the standard and sensor are thermally equilibrated
prior to proceeding with the calibration.

3-point option for maximum accuracy over the entire range of 0 to 200 ug/L. As for the 2-point

menu.

must be 0 NTU. The procedure for this calibration is the same as for a 2-

REESTABILISHING DEFAULT CALIBRATION – “UNCAL” COMMAND

If, for any reason, you want to return to the factory de fault settings (slo pe and offset) fo r any parameter,
follow the instructions below:

• Activate any calibration screen (1-point, 2-point, or 3-point) where a numerical entry is requested. In

the example below, a 2-point turbidity calibration was selected.

• Instead of entering a numeric value, type the word “uncal” and press

• An “uncal” entry at any calibration prompt will reset both slope and offset for this parameter to the

factory default settings.

NOTE: When using the 650 MDS as the sonde interface device, the “uncal” operation is performed by

holding the
details.

Escape key down and then pressing the Enter key. See Section 3 of this manual for more

---------Turbidity calibration--

------­1-1 point
2-2 point
3-3 point

Select option (0 for previous
menu): 2
Enter 1st Turbid NTU:uncal

Enter.

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CALIBRATION RECORD – THE GLP FILE

When any sensor is calibrated, most 6-series sondes will automatically create a file in sonde memory that
provides details of the calibration coefficients before and after the calibration. The file will have a .glp
extension and will have the Circuit Board Serial # as the default filename. The file can be viewed by
following the path File|Directory|File Details|View from the Main sonde menu.

------------------File----------------­1-Directory 4-View file
2-Upload 5-Quick view file
3-Quick Upload 6-Delete all files

Select option (0 for previous menu): 1

Filename Samples
1-BRIDGE1.dat 19
2-BRIDGE2.dat 27
3-UPLAKE.dat 33
4-CLRLAKE2.dat 167
5-DWNLAKE2.dat 31

6 - 00003001.glp 39

-------------File details-------------­1-View file
2-File:00003001.glp
3-Samples: 39
4-Bytes: 790

Pressing 1-View file will show the calibration record for the sonde. An example is shown below:

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m/d/y hh:mm:ss S/N Type Value
08/23/2001 17:07:50 00003001 Conductivity gain 1.000000
08/23/2001 17:07:50 00003001 Cl2 gain 1.000000
08/23/2001 17:07:50 00003001 pH gain (pH-7)*K/mV -5.05833
08/23/2001 17:07:50 00003001 pH offset (pH-7)*K 0.000000
08/23/2001 17:07:50 00003001 ORP offset mV 0.000000
08/23/2001 17:07:50 00003001 TDS constant 0.650000
08/23/2001 17:07:50 00003001 Turb Offset 0.000000
08/23/2001 17:07:50 00003001 Turb A1 500.0000
08/23/2001 17:07:50 00003001 Turb M1 500.0000
08/23/2001 17:07:50 00003001 Turb A2 1000.000
08/23/2001 17:07:50 00003001 Turb M2 1000.000
08/23/2001 17:07:50 00003001 Chl Offset 0.000000
08/23/2001 17:07:50 00003001 Chl A1 100.0000
08/23/2001 17:07:50 00003001 Chl M1 100.0000
08/23/2001 17:07:50 00003001 Chl A2 200.0000
08/23/2001 17:07:50 00003001 Chl M2 200.0000
08/23/2001 17:07:50 00003001 Fluor Offset 0.000000
08/23/2001 17:07:50 00003001 Cl2 gain 1.103424

The data in the display shows a new sonde (Circuit Board # 00003001) which has just had its free chlorine
sensor calibrated. Note that the initial values for all parameters are the default settings. Only the last entry
(Cl2 gain) has been affected by the calibration of the free chlorine sensor. If the conductivity sensor is now
calibrated, the new conductivity gain value is now automatically appended to the record as shown below:

m/d/y hh:mm:ss S/N Type Value
08/23/2001 17:07:50 00003001 Conductivity gain 1.000000
08/23/2001 17:07:50 00003001 Cl2 gain 1.000000
08/23/2001 17:07:50 00003001 pH gain (pH-7)*K/mV -5.05833
08/23/2001 17:07:50 00003001 pH offset (pH-7)*K 0.000000
08/23/2001 17:07:50 00003001 ORP offset mV 0.000000
08/23/2001 17:07:50 00003001 TDS constant 0.650000
08/23/2001 17:07:50 00003001 Turb Offset 0.000000
08/23/2001 17:07:50 00003001 Turb A1 500.0000
08/23/2001 17:07:50 00003001 Turb M1 500.0000
08/23/2001 17:07:50 00003001 Turb A2 1000.000
08/23/2001 17:07:50 00003001 Turb M2 1000.000
08/23/2001 17:07:50 00003001 Chl Offset 0.000000
08/23/2001 17:07:50 00003001 Chl A1 100.0000
08/23/2001 17:07:50 00003001 Chl M1 100.0000
08/23/2001 17:07:50 00003001 Chl A2 200.0000
08/23/2001 17:07:50 00003001 Chl M2 200.0000
08/23/2001 17:07:50 00003001 Fluor Offset 0.000000
08/23/2001 17:07:50 00003001 Cl2 gain 1.103424
08/23/2001 17:23:13 00003001 Conductivity gain 0.979114

Note that the default value of the conductivity “Value” is 1.00 in the .glp format shown above. This
relative number is equivalent to a real cell constant of 5.00 which is provided in the

Advanced|Cal

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Constants and is described in Section 2.9.8. All other values in the .glp file are equivalent to those shown

Advanced|Cal Constants menu.

in the

CAUTION: Calibration records for all sensors will automatically be stored in the .glp file until the Delete
All Files

including the .glp (calibration record) file will be lost. Therefore, it is extremely important to remember to
upload the .glp file to a PC or a 650 Display/Logger prior to deleting files from the sonde. See Section

2.9.3 for instructions on the upload and viewing of the .g lp file.

command is used from the File menu. However, if the Delete command is issued, all files,

2.9.3 FILE

Selections from the File menu allow the user to access data that has been stored in the sonde flash disk
memory. Select

------------------File----------------­1-Directory 4-View file
2-Upload 5-Quick view file
3-Quick Upload 6-Delete all files

Select option (0 for previous menu): 1

Select

1-Directory to view all files currently stored in sonde flash disk memory. The screen below shows

5 files of varying sizes. To examine the details of each file, press the associated number (e.g., 4 for
CLRLAKE2.dat) and an additional screen will be displayed as shown below that quantifies the time of the
deployment, the sample interval, and the site where the sonde was used. In addition, the data in the file can
be viewed by using the

Filename Samples

1-BRIDGE1.dat 19
2-BRIDGE2.dat 27
3-UPLAKE.dat 33
4-CLRLAKE2.dat 167
5-DWNLAKE2.dat 31
6-00003001.glp 3

Select option (0 for previous menu): 4

Select

2-Upload to view file lists in memory (same as shown above) and then upload the data to a PC or to

the YSI 650 MDS Display/Logger. The uploaded data can then be processed with YSI EcoWatch for
Windows to allow data manipulation and to easily generate reports, plots, and statistics. Three formats for
file transfer are available: PC6000, Comma & Quote Delimited, and ASCII text.

3-File from the Main menu.

1-View file command

-------------File details--------

-----­1-View file
2-File:CLRLAKE2.dat
3-Samples: 167
4-Bytes: 4421
5-First:08/23/2001
6-First:08:33:40
7-Last :08/23/2001
8-Last :09:04:20
9-Interval:00:00:10
A-Site:Clear Lake

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• PC6000 format will transfer the data so that it will be compatible with the EcoWatch for Windows

(supplied with your sonde) softwa re package. YSI recommends data transfer in this format since it is
significantly more rapid than other transfer options. If this data is required in Comma & Quote
Delimited and/or ASCII formats, the user can quickly generate data in these formats using the Export
function in EcoWatch for Windows.

Comma & Quote Delimited format is commonly used to generate files that can be imported into

•

spreadsheet software in your PC, where you can perform custom data analysis. A comma is still the
delimiter, but the radix is corrected to a period. In most cases you should set the Page Length to 0
before using this type of upload format. See the Windows Help section in EcoWatch for information
on setting the page length.

ASCII Text is another alternative to transferring data directly to your computer into spreadsheet or

•

other PC-based software.

Prior to upload, a “Time window” display appears to allow you the option to select portions of the logged
data to upload. You may select

--------------Time window-------------­1-Proceed
2-Start date=07/17/96
3-Start time=12:00:00
4-Stop date=07/31/96
5-Stop time=12:00:00

Select option (0 for previous menu):

Select

1-Proceed. Choose the appropriate file transfer protocol. A status box will appear in the lower right

quadrant of the screen. Verification of a successful transfer is indicated when all of the requested data are
transferred.

---------------File type--------------­1-PC6000
2-Comma & ' ' Delimited
3-ASCII Text

Select option (0 for previous menu):

0 or Esc to return to the File menu.

Press

When you select the
only the most recent flash disk file is uploaded and it is uploaded in its entirety. You still must choose the
transfer format from the three options provided.

4-View File to examine the data in any file currently stored in sonde flash disk memory. You will

Select
first view the same screen as viewed in the Directory menu. From this menu choose the file of interest,
then, using the Time window menu, choose the dates and/or times of interest. If you choose dates or times
that are not within the designated start and stop times, no data will be displayed.

3–Quick Upload option, the same operation as 2-Upload is performed, except that

1-Proceed to upload all data logged from the dates and times shown.

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You may choose the entire file. Use the
key to cancel the view.

Select the
This feature is particularly useful in quickly reviewing recently acquired data at field sites so that system
performance can be assessed.

Select
.glp file that may contain valuable calibration data). It is critical not to use this option until all relevant data
from sonde memory has been transferred to your computer via one of the upload options. There is a
verification screen that appears, so that pressing the 6 key does not immediately delete all files at this point.

The management of the .glp calibration record file that is automatically stored in sonde memory and is
described in Section 2.9.2 above is similar to that for data files. However, there are some differences in the
upload procedure of which the user should be aware. When uploading a .glp file there will be a choice of
three upload protocols as shown below:

The binary upload choice should ONLY be used when uploading the .glp file to a 650 Display/Logger; if
the binary protocol is used in a direct upload to PC, a .glp file will indeed be transferred, but it will not be
possible to open it using the current YSI software or any other text editor. Thus, during a direct transfer of
the .glp file to your PC, either the CDF or ASCII formats should be used. The result will be the conversion
of the file with a .glp extension in the sonde to one with a .txt extension which is now stored in the
ECOWWIN\DATA subdirectory of your PC. For example, the file 00003001.glp in the sonde will become

00003001.txt on transfer to PC and the file will be in a format which can easily be viewed and edited using
Notepad or other word processing software.

5–Quick View File option to view the last page of data from the last data file in sonde memory.

6–Delete all files to IRREVERSIBLY remove all files from the sonde memory (INCLUDING the

---------------File type--------------­1-Binary
2-Comma & '' '' Delimited
3-ASCII Text

Select option (0 for previous menu):

Space Bar to alternately stop and to resume scrolling. Use the Esc

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2.9.4 STATUS

Select 4-Status from the Sonde Main menu to obtain general information about the sonde and its setup.

-----------------Status---------------­1-Version:1.00
2-Date=07/22/96
3-Time=09:04:28
4-Bat volts: 9.0
5-Bat life 21.2 days
6-Free bytes:129792
7-Logging:Inactive

Select option (0 for previous menu):

• Select 1-Version to identify the specific version of sonde software loaded in the sonde. This number is

especially useful if you are calling YSI Customer Service. It may also be useful to you if you are
comparing 2 or more sondes purchased at different times.

• Select

2-Date and 3-Time to display current date and time in 24-hour format. This is not a “live”

display, but may be updated by pressing the 2 or 3 key again. This may be useful for viewing or
setting real time. You may correct date or time from this submenu, by entering the corrected date or
time as described in System setup. However, you cannot alter date format from this screen.

• In options 4, 5, and 6, you can view battery voltage, battery life, and available free memory in your

sonde to help you evaluate whether the current setup is appropriate to complete an active logging or to
begin a study in which you have defined your logging parameters. If you press key 4, 5 or 6 before
you exit this screen, it may change, since this triggers the Status screen to update information.

• 7-Logging provides one of two messages, active or inactive, indicating whether your sonde is in the

unattended logging mode. This logging status indicator is not relevant to logging in the discrete mode,
since there is no way to enter the Status screen without stopping discrete logging.

0 or Esc to exit the Status screen and return to Main menu.

Press

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2.9.5 SYSTEM

5-System from the Sonde Main menu to set the date and time, customize the sonde communication

Select
protocol, adjust how information appears on the screen, and enter an instrument identification number and a
GLP file designation.

1-Date & time
2-Comm setup
3-Page length=25
4-Instrument ID=YSI Sonde
5-Circuit board SN:00003001
6-GLP filename=00003001
7-SDI-12 address=0
8-(*)English
9-( )Fran?ais
A-( )Deutsch

Select option (0 for previous menu):

Select Date & time.

-----------Date & time setup----------­1-(*)m/d/y 4-( )4 digit year
2-( )d/m/y 5-Date=08/11/98
3-( )y/m/d 6-Time=11:12:30

Select option (0 for previous menu):

4 and 5 to activate the date and time functions. Pay particular attention to the date format that you

Press
have chosen when entering date. Use the 24-hour clock format for entering time. Option
may be chosen to have the date appear with a two or four digit year.

Press 0 or Esc to return the System Setup menu.

Select

2-Comm setup From the System Setup menu.

4- ( ) 4 digit year

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---------------Comm setup------------­1-(*)Auto baud 5-( )2400 baud
2-( )300 baud 6-( )4800 baud
3-( )600 baud 7-(*)9600 baud
4-( )1200 baud

Select option (0 for previous menu): 0

The default is 9600, but you may change it to match your host communication interface protocol by typing
in the corresponding number, 1 through 7. An asterisk confirms the selection. Auto baud may be selected
along with any of the choices. The Auto baud option allows the sonde to recognize and adjust to the
received characters and we recommend that it is activated.

NOTE:

EcoWatch for Windows, Comm Settings. If you do not adjust the baud rate in th e PC software, the sonde
will not be able to communicate with the computer or any display/logger and your system will appear to be
"locked-up."

Select 3-Page length from the System Setup menu and press Enter. This will allow you to control how
many lines of data are sent to your display before a new header is shown. The smaller the page number, the
fewer the lines of data will be transmitted to your display between headers. However, if you set the page
length to zero (0), only the initial header will be displayed. In many cases, a page length of zero is the
preferred configuration if you choose to upload your data in an ASCII or CDF format. See

File Menu,
NOTE: The header itself takes 4 lines. Therefore, if the page length is set to 25, there will be 21 lines of

data and one header. Any page length less than 5 will result in no header being transmitted.

Select
instrument serial number), and press
serial number of your sonde. This will make sure that any data that is collected is associated with a
particular sonde.

The
entire system as for Instrument ID). Unlike the

SN

Select
calibrate any of the sonde sensors. The default designation for the GLP file is the Circuit Board SN and
this name can be retained if you wish. See section 2.9.3 for general information on the GLP file.

Select
9 and then press
fully described in
communication protocol netwo rk .

Finally, select your language of choice for the sonde menus. Then press
Main menu.

If you change the baud rate, exit the sonde interaction and immediately change the baud rate in

Section 2.9.3,

for more details.

4-Instrument ID from the System setup menu to record the instrument ID number (usually the

Enter. A prompt will appear which will allow you to type in the

5-Circuit Board SN entry shows the serial number of the PCB that is resident in your sonde (not the

Instrument ID, the user cannot change the Circuit Board

.

6-GLP filename to enter a name for the file that is automatically generated and appended as you

7-SDI-12 address from the System Setup menu to change the value. Input a number between 0 and

Enter to confirm the selection. The SDI-12 default address is zero (0). This feature is

Section 7, Communication and only utilized if the unit is to operate in a SDI-12

Esc or 0 again to return to the

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2.9.6 REPORT

The Report menu allows you to configure all reports displayed by the sonde software. You will be able to
select which parameters and units of measure that are displayed during operation.

6-Report from the sonde Main menu. The following menu, or a similar menu, will be displayed.

Select
The parameters listed depend on both the sensors available and enabled on your sonde. Therefore your
screen may not be identical to that shown below.

Select

Report from the Main sonde menu to setup the report section. The Report Setup menu will be

displayed.

-------------Report setup-------------­1-(*)Date A-( )Cl2 chrg
2-(*)Time hh:mm:ss B-( )pH
3-(*)Temp C C-( )pH mV
4-(*)SpCond uS/cm D-(*)Orp mV
5-( )Cond E-( )PAR1
6-( )Resist F-( )PAR2
7-( )TDS G-(*)Turbid NTU
8-( )Sal ppt H-(*)Chl ug/L
9-(*)Free Cl2 I-( )Fluor %FS

Select option (0 for previous menu):

The asterisks (*) that follow the numbers or letters indicate that the parameter will appear on all outputs
and reports.

Select option. The parameters “PAR1” and “PAR2” are associated with a special sonde equipped with a

sensor for Photosynthetically Active Radiation (PAR) which can be purchased from the YSI
Massachusetts, but is not likely to be useful in drinking water studies See
contact information if you have questions about this sensor.

Note that the units of turbidity are automatically presented as “turbid NTU” if a 6026 sensor has been
selected and “turbid+ NTU” if a 6136 sensor has been selected. The different designations are designed to
differentiate the data from the two sensors types in later analysis.

For parameters with multiple unit options such as temperature, conductivity, specific conductance,
resistivity and TDS, a submenu will appear as shown below, allowing selection of desired units for this
parameter.

--------------Select units------------­1-(*)NONE
2-( )Temp C
3-( )Temp F
4-( )Temp K

Select option (0 for previous menu): 2

To turn a parameter on or off type the number or letter, that corresponds to the parameter, after

Section 8 of this manual for

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After configuring your display with the desired parameters, press Esc or 0 to return to the Main menu.

Even if all of the sensors are enabled, the measurements for those sensors will not appear on your display
unless the parameter is selected in Report setup. In order for a specific parameter to show up on a report:

1. The sensor must first be enabled (turned on).

2. That parameter must be activated in the Report setup.
In the above example, if the appropriate sensors have been activated in the Sensor setup section, the

following parameters will be displayed to the computer screen or captured to a computer or data collection
platform when the sonde is sampling: Temperature in C, Specific Conductance in uS/cm, Free chlorine in
mg/L, pH, ORP in millivolts, Turbidity in NTUs and Chlorophyll in ug/L. Date and time will also be
displayed.

NOTE: Do not attempt to memorize or associate a number or letter with a particular parameter. The

numbering scheme is dynamic and changes depending on the sensors which have been enabled.

The following list is a complete listing of the abbreviations utilized for the various parameters and units
available in the Report setup menu.

Parameter Description
Date Day/Month/Year (format selectable)

Time Hour:Minute:Second (24-hour clock format)
Temp C Temperature in degrees Celsius
Temp F Temperature in degrees Fahrenheit
Temp K Temperature in degrees Kelvin
SpCond mS/cm Specific Conductance in milliSiemens per centimeter
SpCond uS/cm Specific Conductance in microSiemens per centimeter
Cond mS/cm Conductivity in milliSiemens per centimeter
Cond uS/cm Conductivity in microSiemens per centimeter
Resist MOhm*cm Resistivity in MegaOhms * centimeter
Resist Kohm*cm Resistivity in KiloOhms * centimeter
Resist Ohm*cm Resistivity in Ohms * centimeter
TDS g/L Total dissolved solids in grams per liter
TDS kg/L Total dissolved solids in kilograms per liter
Sal ppt Salinity in parts per thousand (set to local barometer at calibration)
Free Cl2 mg/L Free chlorine concentration in mg/L
Cl2 chrg Free chlorine sensor charge
pH pH in standard units
pH mV millivolts associated with the pH reading
Orp mV Oxidation reduction potential value in millivolts
NH4+ N mg/L Ammonium Nitrogen in milligrams/liter
NH4+ N mV Ammonium Nitrogen in millivolt reading
NH3 N mg/L Ammonia Nitrogen in milligrams/liter
NO3- N mg/L Nitrate Nitrogen in milligrams/liter
NO3- N mV Nitrate Nitrogen in millivolt reading
Cl- mg/L Chloride in milligrams/liter
Cl- mV Chloride in millivolt reading
Turbid NTU Turbidity in nephelometric turbidity units from 6026 sensor
Turbid+ NTU Turbidity in nephelometric turbidity units from 6136 sensor
Chl ug/L Chlorophyll in micrograms/liter
Fluor %FS Fluorescence in percent Full Scale
Rhod ug/L Rhodamine WT in micrograms/liter

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2.9.7 SENSOR

The Sensor menu allows you to Enable or Disable (turn on or off) any available sensor and, in some cases,
to select the port in which your sensor is installed.

From the Sonde Main menu select

------------Sensors enabled-----------­1-(*)Time 7-( )ISE3 NONE
2-(*)Temperature 8-( )ISE4 NONE
3-(*)Conductivity 9-( )ISE5 NONE
4-(*)Free Cl2 A-( )Optic
5-(*)ISE1 pH B-( )Battery
6-(*)ISE2 Orp

Select option (0 for previous menu):

Note that the exact appearance of this menu will vary depending upon the sensors available on your sonde.

When a particular sensor is active, an asterisk will appear in the parentheses associated with the selection.
In this example the time, temperature, conductivity, free chlorine, pH, ORP, and battery sensors are
enabled. To disable a sensor, simply press the number of the active sensor or port, and the asterisk will
disappear.

For the ISE and Optic selections, press the appropriate number, and then enable or disable the sensor using
the submenu choices. Be certain that the appropriate sensor is “enabled” in the submenu according to the
sonde bulkhead port in which it is physically installed. For example, if an ammonium sensor is placed in
the port labeled “3” on the bulkhead, enable the sensor as ISE3 in the menu structure.

The following screen is the submenu selection structure for ISE3, ISE4 and ISE5.

--------------Select type-------------­1-( )ISE3 NH4+
2-( )ISE3 NO3-
3-( )ISE3 Cl-
4-( )ISE3 PAR1

Select option (0 for previous menu):

As noted above, the ISE3 PAR1 selection is used in a special instrument mated to a sensor for
Photosynthetically Active Radiation that is available from YSI Massachusetts but is not likely to be useful
in drinking water studies. See

A submenu similar to that below will appear if either “Optic T” or “Optic C” is chosen as a sensor.

7-Sensor and the following display will appear.

Section 8 for contact information if you have questions about this sensor.

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--------------Select type-------------­1-(*)Optic-T(or C) Turbidity-6026
2-( )Optic-T(or C) Turbidity-6136
2-( )Optic-T(or C) Chlorophyll
3-( )Optic-T(or C) Rhodamine

Select option (0 for previous menu):

Any available optical probe can be installed in any optical port on YSI 6-series sondes. The single optical
port in the 6920DW will be designated “Optic T” in the software even though the port is not physically
labeled with a “T” on the bulkhead.

2.9.8 ADVANCED

From the Sonde Main menu select 8-Advanced to display the sensor calibration constants, additional setup
options, sensor coefficients and constants, and digital filtering options. The parameters listed depend on
both the sensors installed and the sensors enabled, therefore your screen may not be identical to those
shown below.

----------------Advanced-------------­1-Cal constants
2-Setup
3-Sensor
4-Data filter

Select option (0 for previous menu): 1

Select

1-Cal constants to display the calibration constants, as shown in the following example. Note that

values only appear for the

enabled sensors.

-------------Cal constants------------­1-Cond:5 B-NO3 A:2.543
2-Cl2 gain:1.3048 C-Cl J:99.5
3-mV offset:0 D-Cl S:-0.195
4-pH offset:0 E-Cl A:2.543
5-pH gain:-5.05833 F-Turb Offset:0
6-NH4 J:51.2 G-Turb A1:500
7-NH4 S:0.195 H-Turb M1:500
8-NH4 A:1.092 I-Turb A2:1000
9-NO3 J:99.5 J-Turb M2:1000
A-NO3 S:-0.195

Select option (0 for previous menu): 0

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The following table provides the default value, operating range, and comments relative to the calibration
constants. Error messages will appear during calibration if values are outside the indicated operating range
unless the designation is “not checked”.

Parameter Default Operating range Comments

Cond: 5 4 to 6 Traditional cell constant
Cl2 gain: 1 0.5 to 2.0
mV offset: 0.0 -100 to 100
pH offset: 0.0 -400 to 400
pH gain: -5.0583 -6.07 to -4.22
NH4 J 51.2 Not checked
NH4 S 0.195 0.15 to 0.217
NH4 A 1.092 Not checked
NO3 J 99.5 Not checked
NO3 S -0.195 -0.217 to -0.15
NO3 A 2.543 Not checked
Cl J 99.5 Not checked
Cl S -0.195 -0.217 to -0.15
Cl A 2.543 Not checked
Turb Offset 0 -10 to 10
Turb A1 500 0.6 to 1.5 Range is ratio of M1 to A1
Turb M1 500
Turb A2 1000 0.6 to 1.5 Range is ratio of (M2-M1) to (A2-A1)
Turb M2 1000
Chl Offset 0 -30 to 20
Chl A1 500 0.6 to 1.5 Range is ratio of M1 to A1
Chl M1 500
Chl A2 1000 0.6 to 1.5 Range is ratio of (M2-M1) to (A2-A1)
Chl M2 1000
Rhod Offset 0 -10 to 10
Rhod A1 500 0.6 to 1.5 Range ratio of M1 to A1
Rhod M1 500
Rhod A2 1000 0.6 to 1.5 Range is ratio of (M2-M1) to (A2-A1)
Rhod M2 1000

To reset a calibration cell constant, access the sonde Calibrate menu. Then select the sensor and type
“UNCAL” instead of the value. This action will change that sensors calibration constants back to the
factory default.

From the Advanced menu, select 2-Setup to display miscellaneous options. Type the appropriate number to
activate/deactivate any of the displayed features.

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-------------Advanced setup----------­1-(*)VT100 emulation
2-( )Power up to Menu
3-( )Power up to Run
4-( )Comma radix
5-(*)Auto sleep RS232
6-( )Auto sleep SDI12
7-( )Multi SDI12
8-( )Full SDI12
9-( )Sample and Hold

Select option (0 for previous menu): 0

1-(*)VT100 emulation.

to send escape sequences to clear the screen which in turn results in an improved display. Usually this
feature should be activated, but, if your terminal or terminal emulator shows odd characters at the
beginning of each menu title, then turn this item off. With the feature off, the sonde will send several
carriage returns and line feeds to 'clear' the display. The number of <cr>'s and <lf>'s is determined by the
page length setting.

2-( )Power up to Menu. When this item is enabled, the sonde will go directly to menu mode when power

is applied to the sonde. If the command line mode is not useful for your applications, then enabling this
item will negate the need to type “Menu” and Enter at the # prompt to access the Main sonde menu.
Activation of this item is NOT recommended except for special applications.

3-( )Power up to Run. When this item is enabled the sonde will start sampling and output data as soon

as power is applied to the sonde. If “Power up to menu” is also enabled, the sonde will first enter the menu
mode and then the run mode where it will start sampling. If “Power up to menu” is not enabled, the sonde
will go to command line mode and then start the run function. With “Power up to Run” disabled, the sonde
will wait for your command at power up (in either command line or menu mode) as set by “Power up to
Menu”. If you are using your sonde for Unattended sampling, do not activate this mode. Activation of this
item is NOT recommended except for special applications.

4-( )Comma radix. When this item is enabled, the sonde will replace decimal points with commas when

printing numbers. NOTE: Regardless of this setting, SDI-12 'D' commands will still respond using a
decimal point.

5-(*)Auto sleep RS232. Activation of this feature enables a power savings system when communicating

with the sonde in RS-232 mode. When enabled, power is only applied to the sensors during sampling or
calibration. The most important aspect of this feature is its effect on the free chlorine protocol as described
in Section 2.9.2. For this reason, this feature should be activated for long term monitoring studies in the
RS-232 communication mode and deactivated for sampling studies where the user is present and the sonde
runs continuously. Even with Autosleep inactive, the sonde will “sleep” after one minute with no
communications.

6-(*)Auto sleep SDI12.

with the sonde in SDI-12 mode. This is basically the same as item 5 above except that it is used in
communication via the SDI-12 interface. Also, the sonde will “sleep” in about 100 milliseconds in the
absence of communication, rather that waiting one minute in the Auto sleep RS-232 mode.

Activate this option for VT100 terminal emulation. This feature allows the sonde

Activation of this feature enables the power savings system when communicating

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7-( ) Multi SDI12. Modifies the SDI12 protocol as follows: (1) No SDI12 service request will be issued.

(2) Break commands will not cause a measurement reading to be aborted. Normally, you should leave this
feature “off”.

8-( ) Full SDI12

SDI-12 Verifier. Disabling this feature will allow the unit to be more fault tolerant and will save some
power. We recommend that you leave this feature “off”.

9-( ) Sample and Hold

water sonde is attached to a YSI Process Monitor. When “Sample and Hold” is active, the 6500 display
and the SCADA output of the 6500 becomes equivalent to the internal memory of the sonde while an
Unattended study is running. In the Unattended mode, the sonde “sleeps” between periodic samples with
the interval between samples defined by the user. This method of operation results the extension of both
the sonde battery life (not important in 6500 applications) and the time between required senso r
maintenance procedures (which is importa nt to 6500 drinking water applicati ons) . Aft er a ct i vat i on of the
feature another entry will appear automatically which will allow you to input your sample interval which
defines how often data is transmitted to the 6500 display. This new sample interval will automatically
become the sample interval for the
change the interval until

sample mode will also change to the Sample and Hold interval. In this case, however, you will be able to

change the Unattended Sample interval, but with the caveat that the
change, i.e., the
and the software assures that this will be the case.

REMEMBER: DO NOT ACTIVATE THIS FEATURE UNLESS (A) YOUR SONDE IS ATTACHED
TO A 6500 AND (B) YOU HAVE READ THE DETAILED DESCRIPTION OF THE FEATURE IN

APPENDIX G. USING YOUR DRINKING WATER SONDE WITH A 6500 PROCESS MO N ITOR .

3-Sensor to display and change user-configurable constants as shown in the following display. Type

Select
the appropriate number to change to these parameters.

. Enabling this feature forces full SDI-12 specification in order to pass the NR Systems

. This feature is designed to be activated ONLY for studies in which your drinking

Run|Discrete sample mode of operation and you will not be able to

Sample and Hold is deactivated. In addition, the interval in the Run|Unattended

Sample and Hold interval will also

Unattended sample interval and the Sample and Hold interval MUST be synchronized

------------Advanced sensor----------­1-TDS constant=0.65
2-Cl2 temp co %/C=2.5
7-Cl2 warm up sec=60
8-(*)Wait for Cl2
9-Wipes=1
A-Wipe int=1
B-SDI12-M/wipe=1
C-Turb temp co %/C=0.3
D-(*)Turb spike filter
E-Chl temp co %/C=0

Select option (0 for previous menu):

NOTE: The number of items on this menu depends greatly on the sensors that are available and enabled on

your sonde. Below we describe every possible item on this menu. Your sonde probably may not have
every item described below.

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To edit one of the following menu items choose the number or letter that corresponds to it.

TDS constant=0.65

Cl2 temp co=2.5 %/C This selection allows you to input the free chlorine temperature coefficient. Do

Cl2 warm up=60 This selection allows you to set the amount of time allowed for Cl2 warm up in seconds.

(*)Wait for Cl2 When this feature is enabled, the sonde is forced to wait for the Cl2 warm up time to

Wipes=1 If a turbidity, chlorophyll, or rhodamine WT sensor is enabled, this selection will

Wipe Int=1 In applications where a turbidity, chlorophyll, or rhodamine WT probe is in stalled in the

This selection allows you to set the constant used to calculate TDS. TDS in g/L is

calculated by multiplying this constant times the specific conductance in mS/cm. This
item will only appear if the conductivity sensor is enabled in the “Sensors enabled”
menu. See Section 5, Principles of Operation for m ore in fo rmation on the TDS
parameter.

not change this value unless you consult YSI Customer Service. This item will only
appear if the free chlorine sensor is enabled in the Sensors menu.

Normally the default value of 60 seconds is adequate for most applications. However,
there may be certain situations in which greater free chlorine accuracy can be attained by
increasing this time. Consult YSI Customer Service if you feel that your Cl2 warm up
time is incorrect. This item will only appear if the free chlorine sensor is enabled in the
Sensors menu.

expire before displaying any readings. Note that in SDI12 mode or while calibrating the
Cl2 sensor, the warm up time is used regardless of the activation of this item. Disabling
this item allows you to see data without having to wait during the Cl2 warm up time.

Under normal operating conditions, this item should be turned off. If you are using

the sonde with a data logger in RS232 mode and will be turning the sonde “on ” and “off”
for each sample, then you may want to enable this item so that only stable Cl2 data are
recorded. This item will only appear if the Cl2 sensor is enabled in the Sens ors menu.

determine the number of cleaning cycles which will occur when the wiper is activated
manually or automatically. Since the wiper functions bidirectionally, a selection of “1”
results in two passes of the wiper over the optical face. In most applications, a single
cleaning cycle is adequate to keep the optical surface free of bubbles and fouling.
However, in particularly harsh environments additional cleaning cycles may be needed
and can be selected here. This item will only appear if a turbidity, chlorophyll, or

rhodamine WT sensor is enabled in the Sensors menu.

sonde and the instrument is collecting data in the SDI-12 communication mode, the wiper
mechanism of the probe should be activated automatically in a periodic manner to clean
the optical surface for fouling and bubbles. The value entered at this selection is the
number of minutes
“5” and the instrument is in the Run mode, the wiper will activate every 5 minutes with
no manual input. This item will only appear if a turbidity, chlorophyll, or rhodamine WT
sensor is enabled in the Sensors menu.

The value of Wipe Int is sometimes overridden when the instrument is set up in the
Unattended sampling mode. Under these conditions, the wiper will be automatically
activated at the interval assigned in the Unattended setup rather than that assigned in
Wipe Int. Thus, in an Unattended study setup at a 15 minute sampling interval, the wiper
will be activated only once every 15 minutes rather than at the indicated Wipe Int of 1
minute.

between each automatic cleaning cycle. Thus, if Wipe Int is set to

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CAUTION: If Wipe Int is set to zero, then no wiping will occur either in Discrete or
Unattended Sampling. Make certain that Wipe Int is set to some finite value prior to
setting up an Unattended study or no automatic cleaning will occur.

SDI12-M/Wipe=1 This is the number of wiping cycles when the sonde is in SDI12 mode. The wiper for

the 6026 and 6136 turbidity, 6025 chlorophyll, and 6130 rhodamine WT sensors will
automatically wipe each time this many SDI12 “M” commands have been issued. If this
value is set to zero, then no automatic wiping will occur. This item will only appear if a
turbidity, chlorophyll, or rhodamine WT sensor is enabled in the Sensors menu.

Turb temp co %/C=0.3 This entry sets the coefficient for the temperature compensation of turbidity

readings from the 6026 and 6136 sensors. The default values of 0.3 (6026) and 0.6
(6136) should not be changed by the user without consulting YSI Customer Service. This
item will only appear if a turbidity sensor is enabled in the Sensor menu.

(* ) Turb Spi

Chl temp co %/C=0.0 This entry sets the coefficient for the temperature compensation of chlorophyll

From the Advanced menu, select
activate/deactivate any of the displayed features.

Sondes with
following menu.

ke Filter When this item is activated, the output of the turbidity sensor is mathematically

processed to minimize the effect of unusual (or “bad”) readings on the overall data
presentation. In most cases, these “spike” events are the result of the chance passage of
large suspended particles across the probe optics just at the time a reading is taken.
Activation of this option generally results in a better display of the “average” turbidity of
the water under examination and its use is recommended for most sampling and
unattended applications. This item will only appear if a turbidity sensor is enabled in the
Sensors menu.

readings from the 6025 sensor. The default value of zero should only be changed by the
user after establishing the temperature compensation factor for the phytoplankton sample
in question. See Section 5.12 of this manual for more information. This item will only
appear if a chlorophyll sensor is enabled in the Sensors menu.

4-Data filter to display filtering options. Type the appropriate number to

no optical (turbidity, chlorophyll, or rhodamine WT) probes enabled will display the

------------Data filter setup---------­1-(*)Enabled
2-( )Wait for filter
3-Time constant=4
4-Threshold=0.001

Select option (0 for previous menu):

If any optical probe (turbidity, chlorophyll, or rhodamine WT)
follows.

is enabled, then the menu will appear as

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------------Data filter setup---------­1-(*)Enabled
2-( )Wait for filter
3-Time constant. . .
4-Threshold. . .

Select option (0 for previous menu):

Choosing 3-Time Constant will display the following:

------------Time constant ---------­1- Turbid=12
2- Chl=12
3- Other=4

Select option (0 for previous menu):

Recommended settings for time constants are turbidity 12, rhodamine WT 12, chlorophyll 12, and “other”

4. Note that the time constant can be set independently for turbidity, chlorophyll, and rhodamine WT and
that time constant choices are only available for activated sensors. Rhodamine WT would appear in the
display below if it had been activated as a sensor. All “other” sensors use the same time constant as shown
below.

Choosing

Setting thresholds is done in the same manner. Recommended threshold settings are 0.01 for turbidity, 1.0
for chlorophyll, 1.0 for rhodamine WT, and 0.001 for “other”.

The following descriptions provide additional information about the Data Filter feature.

1-(*) Enabled. Activating this item will result in data filtering according to the values set in (2), (3), and

(4).

2-(*) Wait for filter. If this feature is activated, readings will be available for output only after the unit has

warmed up for a time period equal to the
example, if you are operating in SDI12 mode and want to average the data over a particular period of time.
In such a case, you would not want the filter to be engaging and disengaging, so the value of the
should be set to a large value like 1. This feature should not be activated for normal use of the sonde.

4-Threshold will display the following:

---------------Threshold-------------­1-Turbid=0.01
2-Chl=1
3-Other=0.001

Select option (0 for previous menu):

Time Constant plus an extra 4 seconds. This feature is useful, for

Threshold

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