UVP iBox Spectra Small Animal Imaging System User Manual

iBox

Spectra™

Small Animal Imaging System

®

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

81-0304-03 Rev D

iBox Spectra Imaging System 2

Table of Contents

Table of Contents ......................................................................................................................................................... 2

Introduction .................................................................................................................................................................. 3

System Components ................................................................................................................................................... 4

Optional Equipment ................................................................................................................................................... 6

Setup Instructions ........................................................................................................................................................ 7

Components .............................................................................................................................................................. 7

Installing the Warming Plate ...................................................................................................................................... 7

Installing Emission Filters .......................................................................................................................................... 8

Installing the BioLite MultiSpectral Light Source ........................................................................................................ 8

Using the System ....................................................................................................................................................... 10

Powering Up the System ......................................................................................................................................... 10

Operating the TS2 Software Interface ..................................................................................................................... 10

Selecting Emission Filters ....................................................................................................................................... 11

Using the Overhead (Epi) Lighting .......................................................................................................................... 11

Using the Warming Plate ......................................................................................................................................... 11

Using the Sample Viewer Window .......................................................................................................................... 11

Image Focusing ....................................................................................................................................................... 11

Setting the White Balance ....................................................................................................................................... 11

Service Procedures .................................................................................................................................................... 13

Return Procedure .................................................................................................................................................... 13

Replacement Parts and Accessories ....................................................................................................................... 13

Troubleshooting ....................................................................................................................................................... 13

Technical Support ................................................................................................................................................... 14

iBox Spectra Imaging System 3

Introduction

The iBox Spectra Small Animal Imaging System is a high resolution imager designed as a quick
screening device for accurate, repeatable imaging of small animals. The self-contained im ag ing sy ste m
contains a built-in 15.6” touch screen and computer as well as a light-tight darkroom with TS2 Software for
image acquisition. In addition, the iBox Spectra comes equipped with a warming plate to help maintain the
animal at a constant 37°C. The iBox offers a color camera and motorized lens for in vivo applications,
providing real-time, live prev ie w images.

Included as standard with the system is a Manual BioLite which offers a direct lighting source using fiber
optic bundles to tightly control the output spectrum for consistent, repeatable measurements. The Manual
BioLite utilizes matched excitation (455-495nm) and emission (515 long pass) filters for visualization of
small animals.

The darkroom has a sample viewer window built into the darkroom door, integrated overhead epi white
lighting for sample placement, and a motorized, five-position slide-out emission filter wheel. Images can be
saved to the internal hard drive, to an external USB storage device or to a wired or wireless network drive
for later analysis or enhancement for publication.

iBox Spectra Imaging System 4

Camera and Lens

Hood

15.6” LCD Touch

Main Power Button

Wide Access Door

USB and SD Card

Access Port

Built-In Overhead

White Lights

Five Position Slide-

Wheel

Sample Viewer

Window

Safety Interlock

Switch & Override

BioLite™ Access

Power Indicator

Lights

Roll-Out

System Components

Refer to the packing slip and pictured components for specific parts and com pon ents included with the
system.

Built Into Darkroom

Warming Plate Tray

Specifications

Power Requirements: 100/115V, 50/60Hz; 3.1 Amps at 120 Volts
230V, 50/60Hz; 1.55 Amps at 230 Volts
Mains supply voltage fluctuations are not to exceed 10 percent of the

nominal supply voltage

Pollution Degree: 2
Installation Category: II
Altitude: Up to 2000m
Ambient Temperature: 5°C to 40°C
Humidity: Maximum relative humidity of 80% for temperatures up to 31°C,

decreasing linearly to 50% maximum relative humidity at 40°C

Built-In Touch Screen Computer

Operating System: Windows® 7, 64-Bit
Connectivity Ports: 2 USB, 1 SD/SDHC/MMC (side of system)
4 USB, VGA and Ethernet (rear of system)
Wireless Networking Capability: 802.11 b/g/n
USB Flash Drive Capacity: 2 GB (minimum)
Internal Hard Drive: 250 GB (minimum)
Software: TS2 (Touch Screen) Software

Screen with Built-In

Computer

Out Emission Filter

iBox Spectra Imaging System 5

TS2 Software Screen

Camera and Lens

The iBox Spectra is equipped with the MultiCam 310C camera, a scientific-grade color CCD camera
with a resolution of 2.0MP (1600x1200). The MultiCam 310C is equipped with a 12.5-75mm f/1.2 zoom
lens.

All camera settings are factory pre-set for optimum performance when viewing samples under low light
conditions. Contact UVP Technical Support prior to making any adjustm ents to the camera settings.

Darkroom

The darkroom is light tight to provide optimal imaging conditions. Darkroom features include:

 Epi (overhead) LED white light
 Roll-out warming plate tray
 Sample viewer window built into the darkroom door
 Five position emission filter wheel
 Brackets and built-in power ports for installing optional epi illumination lamps
 Safety interlock switch with manual override to disable UV when darkroom door is opened

LCD Touch Screen

The iBox Spectra contains a fully integrated 15.6-inch color touch screen. The touch screen allows the
user to perform a variety of tasks, including previewing, capturing, saving and printing images as well as
selecting preference options, without the use of an external mouse or keyboard.

For users who prefer not to use the touch screen interface, an external keyboard and mouse are
included as standard. These components can be plugged into any available system USB port.

Warming Plate

The warming plate houses electrical heating elements which maintain the surface of the warming plate
at a constant temperature of 37°C to keep animals warm during the imaging process. The warming
plate plugs into a dedicated port inside the iBox darkroom.

BioLite™ MultiSpectral Light Source

The Manual BioLite Multispectral Source is a 150-Watt quartz halogen visible light source designed for
use with ferrule fiber optic bundles. It features a closed optical path to tightly control the output
spectrum, allowing consistent and repeatable measurements with superior signal to nois e. T he BioLit e
features a six-position dimmer permitting variable intensity. The user inserts the excitation filter into the
slot at the front of the BioLite to control the wavelength of light emitted.

TS2 Software

Image analysis and hardware functions for the iBox Spectra
are controlled by the TS2 (Touch Screen) Software interface.
In addition to image preview, capture and save functions, the
TS2 Software controls camera and lens functions (exposure,
aperture, focus and zoom), image printing, lighting and filter
selection, and other user preference s.

For additional information, refer to the TS2 Software User
Manual included with the system or available online at
http://uvp.com/manuals.