UVP ChemiDoc-It 2 Imagers User Manual

GelDoc-It®2 & ChemiDoc-It®2 Imagers

Installation and User Instructions

UVP, LLC	Ultra-Violet Products Ltd.
2066 W. 11th Street	Unit 1, Trinity Hall Farm Estate
Upland, CA 91786	Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone]	Phone: [phone]
Fax: [phone]	Fax: [phone]

Web Site: www.uvp.com

81-0220-01 Rev N

GelDoc-It2 and ChemiDoc-It2 Imagers	2
Table of Contents
Table of Contents.........................................................................................................................................................	2
Introduction ..................................................................................................................................................................	3
System Components ...................................................................................................................................................	4
Minimum Computer Requirements............................................................................................................................	4
Cameras....................................................................................................................................................................	5
Optional Equipment...................................................................................................................................................	6
Setup Instructions........................................................................................................................................................	8
Components..............................................................................................................................................................	8
Connecting the Power Cables...................................................................................................................................	8
Installing the Emission Filter......................................................................................................................................	8
Camera Setup ...........................................................................................................................................................	9
Software Installation ................................................................................................................................................	12
Using the System .......................................................................................................................................................	15
Powering Up the System.........................................................................................................................................	15
Using the Filters ......................................................................................................................................................	15
Using the Transilluminator.......................................................................................................................................	16
Using the Overhead (Epi) Lighting ..........................................................................................................................	16
Using the UV Gel Viewer Window ...........................................................................................................................	16
Capturing Images and Using Templates..................................................................................................................	17
Image Focusing.......................................................................................................................................................	17
Capturing Images....................................................................................................................................................	17
Using Templates .....................................................................................................................................................	18
Service Procedures....................................................................................................................................................	20
Return Procedure....................................................................................................................................................	20
Replacement Parts and Accessories.......................................................................................................................	20
Troubleshooting.......................................................................................................................................................	20
Care and Cleaning ..................................................................................................................................................	21
Technical Support ...................................................................................................................................................	21

GelDoc-It2 and ChemiDoc-It2 Imagers

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Introduction

The GelDoc-It2 Imager is designed as a high resolution imager capable of capturing, documenting and analyzing fluorescent gel images.

In addition to gel documentation capabilities, the ChemiDoc-It2 Imager is equipped with a cooled, scientificgrade CCD camera allowing users to capture, document and analyze chemiluminescent blot images, including Western blots.

The GelDoc-It2 and ChemiDoc-It2 Imagers incorporate a light-tight darkroom with VisionWorks®LS software for image acquisition and analysis functions. The darkroom has a UV-blocking gel viewer window, built-in overhead epi white lighting, a UV transilluminator and a four-position emission filter wheel with an ethidium bromide (EtBr) emission filter included as standard.

GelDoc-It2 and ChemiDoc-It2 Imagers

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System Components

Refer to the packing slip and pictured components for parts included with the system.

Four Position

Emission Filter

Selector

Access port for optional BioLite™ MultiSpectral Light Source

Epi Overhead

White Light

UV-Safe Gel

Viewer

Window

Darkroom

Access Door

Camera and Lens Kit (actual configuration may be different than shown in the photo)

Lighting and UV

Timer Switches

UV Safety

Interlock Switch

LED White Light

Plate (Optional)

Roll-Out

Transilluminator

Minimum Computer Requirements

Processor:	Pentium class, 1.6 GHz or higher
RAM:	2 GB (4 GB Preferred)
Display:	1024 x 768
Operating System:	Microsoft Windows XP Service Pack 2 or Newer (32-bit or 64-bit)
Internet Browser:	Microsoft Internet Explorer 6.0 or later
Computer USB Ports:	Computer must come equipped with a minimum of three USB
	ports; additional peripherals (mouse, keyboard, printer, etc.) may
	require additional USB ports.

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Cameras

GelDoc-It2: The GelDoc-It2 is equipped with the GelCam 310 camera, a scientific-grade monochrome CCD camera with a resolution of 2.0MP (1600x1200) with USB 2.0 PC interface.

ChemiDoc-It2: The ChemiDoc-It2 is equipped with the BioChemi™ 510, OptiChemi™ 610 or

MegaCam 810 scientific-grade monochrome CCD camera. All cameras are Peltier cooled and offer full 16-bit file bit depth:

The BioChemi 510 camera has 2.1MP resolution with Peltier cooling to -35°C from ambient. The BioChemi 510 has a peak quantum efficiency of 50% and is capable of binning from 1x1 to 8x8.

The OptiChemi 610 camera has 3.2MP (2184x1472) resolution with Peltier cooling to -50°C from ambient. The OptiChemi 610 has a peak quantum efficiency of 86% and is capable of binning from 1x1 to 10x10.

The MegaCam 810 camera has 8.1 MP (3296x2472) resolution with Peltier cooling to -35°C from ambient. The MegaCam 810 has a peak quantum efficiency of 50% and is also capable of binning from 1x1 to 8x8.

All camera settings are factory pre-set for optimum performance when viewing gels, films or membranes under low light level conditions. Contact UVP Technical Support prior to making any adjustments to the camera settings.

Lens Kit (Motorized or Manual)

The zoom lens used with the GelCam 310 and BioChemi 510 cameras comes in two configurations:





The motorized lens is controlled via VisionWorksLS Software. The manual lens requires manual user adjustment.

Some lenses may be fitted with a close-up diopter. The diopter is used for focusing on objects at the focal length of the GelDoc-It2/ChemiDoc-It2 darkroom.

Ethidium Bromide (EtBr) Emission Filter

The ethidium bromide (50mm square) UV-blocking bandpass interference filter blocks UV and IR radiation emitted from the transilluminator. The filter is placed in the filter wheel below the camera assembly. The filter allows visualization of fluorophores from 580-630nm, targeting the ethidium bromide emission peak of 605nm.

Additional filters are available for other specific fluorophores. Filters can also be removed when imaging non-fluorescent media (including chemiluminescent blots, protein gels, colony plates, etc.) in order to produce brighter images. Contact UVP for ordering information.

Darkroom

The darkroom is light tight to provide optimal imaging conditions. Darkroom features include:

Overhead white light

Roll-out transilluminator tray

UV-safe gel viewer window built into the darkroom door

Side pocket for storage of materials

Four position emission filter wheel

Brackets for installing optional UV handheld lamps for epi (overhead) UV illumination

Built-in power ports for transilluminator and optional overhead UV handheld lamps

Built-in power cord for optional LED White Light Plate

UV safety interlock switch with manual override to disable UV transillumination and optional UV epi illumination when darkroom door is opened

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Transilluminator

The GelDoc-It2 and ChemiDoc-It2 Imagers can accommodate UVP’s Benchtop and FirstLight transilluminator models. UVP offers a variety of transilluminator configurations, including Benchtop models with multiple wavelengths and variable intensities as well as the highly uniform, patented FirstLight® transilluminator. Refer to the packing slip for the transilluminator included with your system.

Note: For UV protection and to extend the life of the UV transilluminator, the system incorporates a tenminute transilluminator shutoff timer. The timer shuts off the transilluminator after ten minutes of operation. A push button on the front of the darkroom allows the user to reset the timer. Alternately, the timer can also be reset by opening and closing the darkroom door.

Fluorescent Focus Target

The UVP Fluorescent Focus Target fluoresces when placed on a UV transilluminator or when exposed to overhead UV. The Target provides sharp fluorescent images to aid in adjusting the lens and camera settings for ideal imaging results.

Fluorescent Focus Target

Optional Equipment

UVP offers a variety of optional equipment to support the needs of varying laboratory environments. Refer to “Replacement Parts and Accessories” at the end of this manual for optional equipment part numbers.

Thermal Printer

The thermal printer provides archive quality, 256 grayscale prints and five optional cost-effective print sizes.

Thermal Printer

UV Handheld Lamps for Overhead Lighting

Two 4-watt ultraviolet lamps can be connected inside the darkroom to provide epi UV illumination. These lamps can be switched on or off using the switch on the front of the darkroom and can also be removed from the darkroom and used as standalone handheld lamps (as seen in the image to the right).

UV Lamp

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LED White Light Plate

The LED White Light Plate emits high uniformity with less than 5% coefficient of variance (CV). Plug the LED White Light Plate directly into the power supply within the system darkroom, or store it in the exterior darkroom side pocket when not in use.

LED White Light Plate

Converter Plates

An alternate to the LED White Light Plate, the UV/White Converter Plate allows imaging of non-fluorescent stained media with an ultraviolet transilluminator. The converter plate is specially coated to convert 302nm UV output to white light rather than using a separate white light box or plate.

The Visi-Blue™ Converter Plate (not shown) converts UV to a safe 460-470nm blue wavelength designed for use with blue excitation samples and SYBR Green, SYPRO Orange and GFP stains.

UV/White Converter Plate