UVP GelDoc-It TS Imaging System User Manual

GelDoc-It™ TS Imaging System

Installation and User Instructions

UVP, LLC	Ultra-Violet Products Ltd.
2066 W. 11th Street	Unit 1, Trinity Hall Farm Estate
Upland, CA 91786	Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone]	Phone: [phone]
Fax: [phone]	Fax: [phone]
	Web Site: www.uvp.com
	81-0259-01 Rev. K

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Introduction

The GelDoc-It TS System is designed as a self-contained imaging system with a built-in computer, no external computer required. The system enables capture and documentation of fluorescent gels with the ability to save images to the internal system or to an external USB storage device for later quantitative analysis or enhancement for publication.

System Components

Components

Refer to the packing slip and pictured components for parts included with the system.

LCD Touch Screen

Main Power Switch

USB Drive

Viewport

Computer

Operating System:

Processor:

USB storage device capacity:

Internal storage (hard disk):

USB 2.0 ports:

Software Options:

Gel Camera with Lens Kit (hidden behind the LCD touch screen)

Filter Tray (located under the camera) with EtBr Filter

Power switches

UV safety Override

Transilluminator on roll-out tray

Windows XP 1.5GHz (minimum) 1G (minimum) 60GB (minimum)

1 front, 4 in the back

TS software (supplied only with the manual lens)

VisionWorks®LS software (supplied with the motorized lens)

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Camera

GelCam 310 Camera, CCD monochrome model with a resolution of 1600 x 1200 with a USB 2.0 PC interface. All camera settings are factory pre-set for optimum performance when viewing gels, films, or membranes under low light level conditions. UVP Technical Support should be contacted before making any adjustments to the camera settings.

Lens Kit (Motorized or Manual)

The zoom lens comes in two configurations.

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The motorized version is controlled via the computer.

The manual version needs manual adjustment from the user.

Both lenses are fitted with a close-up diopter. The diopter is used for focusing on objects at the focus length of the GelDoc-It TS System.

Ethidium Bromide Filter (EtBr)

The Ethidium Bromide (EtBr) Filter (50mm sq.) UV blocking band pass interference filter blocks UV and IR radiation emitted from the transilluminator. The filter is placed in the filter tray below the camera assembly. The filter allows visualization of fluorophores from 580-630nm, targeting the EtBr emission which peaks at 605nm. The EtBr filter can be substituted for other specific fluorophore filters or removed when imaging non-fluorescent media (protein gels, colony plates, etc.) in order to produce brighter images.

Darkroom

The darkroom is light tight for optimum imaging conditions. The darkroom features:

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Overhead white light

Roll out tray for the transilluminator Gel viewer window

Side pocket for storage of materials

Brackets for installing optional UV lamps for overhead UV illumination Built in power cord for an optional LED white light tray

Transilluminator

A transilluminator may be included with the system; refer to the packing list. The GelDoc-It TS System can accommodate UVP’s Benchtop and FirstLight transilluminator models. UVP offers a variety of configurations, from the benchtop models with 8-watt, single wavelength and single intensity to multiple wavelengths and high/low intensities, or the high-end FirstLight transilluminator.

Note: For UV protection, there is a 10-minute timer installed in the system. The timer shuts off the transilluminator after 10 minutes of operation. A switch on the front of the darkroom allows the user to reset the timer. When the darkroom door is opened, this automatically resets the timer.

LCD Touch Screen

The display is a 15-inch VGA color touch screen connected to the darkroom cabinet with tilt adjustment. The touch screen allows the user to preview, snap, save and print images, as well as select certain preference options, without the need of an external mouse or keyboard.

A stylus pen is included for increased pointer control in selecting software options on the touch screen.

Focus Target

The focus target fluoresces when placed on a transilluminator or when exposed to overhead UV. The target provides sharp, fluorescent images to aid in adjusting the focus of the camera.

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Optional Equipment

Refer to the ordering information section of this manual for optional equipment part numbers.

Thermal Printer

The thermal printer provides archive quality, 256 grayscale prints, and five optional cost-effective print sizes.

UV Handheld Lamps for Overhead lighting	Printer

A set of two 4-watt ultraviolet lamps can be connected inside the darkroom for UV epi-illumination. The lamps can be switched On/Off using the switch on the front of the darkroom.

Converter Plates

The White Light Tray plugs into the darkroom for uniform white light transillumination.

The UV/White Converter Plate allows imaging of nonfluorescent stained media with an ultraviolet transilluminator. The converter plate is specially coated to convert the 302nm UV output to white light, rather than using a separate white light box.

The Visi-Blue Converter Plate converts UV to 460-470nm designed for use with blue excitation samples and SYBR green, SYPRO orange and GFP stains.

Transilluminators

If the system does not include a transilluminator (check the packing slip), contact UVP for ordering information or go to UVP’s web site at www.uvp.com. A large selection of transilluminators is available from UVP. These units include compact 8-watt models to our high performance FirstLight Transilluminator.

VisionWorksLS Analysis Software

UV Lamp

UV/White Converter Plate

The GelDoc-It TS System can be configured with VisionWorks®LS software for analysis of gels, plates, and membranes. The software features image enhancement, complete analysis tools and reporting capabilities.

Mouse and Keyboard

An external keyboard and mouse can be connected to the GelDoc-It system. These components connect to any available USB port in the back of the darkroom.

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Set-up Instructions

Components

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Camera with zoom lens, bracket, filter, cables GelDoc-It TS darkroom

Windows XP operating system and software to control the GelDoc-It TS USB storage device, 64MB (minimum)

Transilluminator (may be ordered separately)

WARNING: DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE EQUIPMENT IS CONNECTED TO ANY POWER SUPPLY

CAUTION

Do not install the system in places with high moisture, dust, or high temperature. Do not use any oil or petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that the system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic equipment apparatus.

Installing the Filter

To install the 50mm sq Ethidium Bromide (EtBr) filter:

1.Carefully remove the filter from the protective plastic case, holding the filter at the edges to prevent fingerprints.

2.The filter tray is located under the camera bracket on the right side top of the darkroom. Slide the tray open.

3.Place the filter inside the filter tray so that the text on the edge of the filter is positioned so the letters are right side up when facing you.

Additional filters are available. Refer to Accessories for ordering information.