UVP BioDoc-It System User Manual

BioDoc-It® Imaging System

Installation and User Instructions

UVP, LLC	Ultra-Violet Products Ltd.
2066 W. 11th Street	Unit 1, Trinity Hall Farm Estate
Upland, CA 91786	Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone]	Phone: [phone]
Fax: [phone]	Fax: [phone]

Web Site: www.uvp.com

81-0301-01 Rev H

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System Introduction

BioDoc-It® Imaging System

The BioDoc-It Imaging System enables simple documentation of fluorescent and non-fluorescent gels, membranes, blots, film plates and assays with the ability to save images to a USB storage device for later quantitative analysis or enhancement for publication.

Images are saved in 8-bit TIFF or JPEG formats, allowing saved images to be read by most PC or MAC programs. The BioDoc-It Imaging System is a cost effective solution for capturing quality images in a compact standalone package, as no external computer is required.

The BioDoc-It comes in two configurations:

BioDoc-It 220 Imaging System includes a high resolution, 1.3 megapixel camera, 8-48mm zoom lens and side access doors.

BioDoc-It 210 Imaging System includes a 0.3 megapixel camera and an 8-48mm zoom lens.

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System Components

BioDoc-It Components

Refer to the packing slip and pictured components for parts included with the system.

8” LCD Touch Screen with Tilt Capability

USB Drive

Wide Access

Door

UV Gel Viewer

Window

FluorCam 210 or 220 Camera and Zoom Lens

Filter Tray (located under the camera) with EtBr Filter

Main System Power Button

White Light and Darkroom

Power Switches

UV to White Light Converter Plate (order separately)

Side Access Doors (on BioDoc-It 220 models)

Transilluminator

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System Specifications

Camera

BioDoc-It 220 Models:

The BioDoc-It 220 is equipped with a FluorCam 220

Camera (resolution of 1.3MP).

BioDoc-It 210 Models:

The BioDoc-It 210 is equipped with a FluorCam 210

Camera (resolution of 0.3MP).

Both the FluorCam 220 and FluorCam 210 use a USB 2.0 PC connection. All camera settings are factory pre-set for optimum performance when viewing gels, films, or membranes under low light level conditions. Contact UVP Technical Support before making any adjustments to the camera settings.

Lens

The BioDoc-It is equipped with an 8-48mm zoom lens fitted with a close-up diopter and step-up ring, allowing the camera to be held in a fixed position. The diopter is for focusing on objects at the focus length of the BioDoc-It Imaging System’s darkroom.

Built-In Computer

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Internal Storage: 60GB or more

USB 2.0 Ports: 1 front, 4 in the back

USB Storage Device Capacity: 1GB (minimum)

Software: TS software

Ethidium Bromide Filter (EtBr)

The Ethidium Bromide (EtBr) filter (50mm sq) UV blocking band pass interference filter blocks UV and IR radiation emitted from the transilluminator. The filter is placed in the filter tray below the camera/lens assembly.

The filter allows visualization of fluorophores from 580-630nm, targeting the EtBr emission peak of 605nm.

The EtBr filter can be substituted for other specific fluorophore filters or removed when imaging nonfluorescent media (protein gels, colony plates, etc.) in order to produce brighter images.

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Darkroom

Darkroom features include:

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UV gel viewer window Overhead white light

Wide access door with UV safety interlock switch

Transilluminator

A transilluminator is included with the BioDoc-It system. UVP offers a variety of transilluminators, from benchtop models with 8-watt, single wavelength and single intensity to models with multiple wavelengths and high/low intensities. UVP also offers the high-end FirstLight®, a highly uniform, 302nm UV transilluminator.

LCD Touch Screen

The display is an 8-inch VGA color touch screen with tilt adjustment connected to the darkroom cabinet. The touch screen allows the user to preview, snap, save and print images, as well as select certain preference options, without the need to use an external mouse or keyboard. However, should the user desire, a mouse and/or keyboard (not included) can be used with the BioDoc-It.

A stylus pen is included for increased pointer control for selecting software options on the touch screen.

UVP Focus Target

The UVP Focus Target fluoresces when placed on a transilluminator or when exposed to overhead UV. The target provides sharp, fluorescent images to aid in adjusting the focus of the camera.

Removable USB Stick

The removable USB stick, included with the system, has 8GB memory (minimum) and connects to the system to allow for saving and transferring of images.

UVP Focus Target

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Optional Equipment

Refer to the Replacement Parts and Accessories section of this manual for optional equipment part numbers.

Thermal Printer

The Thermal Printer provides archive quality, 256 grayscale prints and five optional cost-effective print sizes.

Thermal Printer

Converter Plates

The UV/White Converter Plate allows imaging of non-fluorescent stained media with an ultraviolet transilluminator. The converter plate is specially coated to convert 302nm UV output to white light.

The Visi-Blue™ Converter Plate (not shown) converts UV to 460470nm and is designed for use with blue excitation samples such as SYBR Green, SYPRO Orange and GFP stains.

UV/White Converter Plate