UVP iBox Scientia Small Animal Imaging System User Manual

Ultra-Violet Products Ltd.

iBox® Scientia™
Small Animal Imaging System

Instruction Manual

UVP, LLC

2066 W. 11th Street, Upland, CA 91786
Tel: [phone]
Fax: [phone]

Web Site: www.uvp.com

Tel: [phone] Fax: [phone]

Nuffield Road, Cambridge CB4 1TG UK

Unit 1, Trinity Hall Farm Estate

81-0304-01 Rev J

iBox Scientia Small Animal Imaging System 2

Introduction

The iBox Small Animal Imaging System is designed to automate research with one-touch, pre-s et or user­defined PC controls for accurate, repeatable imaging of small animals.

The iBox system incorporates the light tight darkroom, BioLite and matched emission/excitation green
fluorescent protein (GFP) and red fluorescent protein (RFP) filter sets.

The darkroom has a UV blocking window, built-in overhead 365nm UV, Visi-Blue™ 480nm and white light,
five-position emission filter array, and a software controlled power lift.

The iBox system comes equipped with a warming plate with internal heating elements that keep the surface
of the warming plate at a constant 37°C.

The BioLite offers an eight position filter wheel, six position intensity selector, and a direct lighting source
using fiber optic bundles to tightly control the output spectrum for consistent, repeatable measurements.

The iBox offers a highly sensitive CCD came r a for in vivo applications and supplies real time, live preview
images.

iBox Small Animal Imaging System

shown with the Automated BioLite MultiSpectral Source

iBox Scientia Small Animal Imaging System 3

Components

The iBox Imaging System is comprised of the following equipment:

 Darkroom Cabinet
 Automated Filter Wheel with Emission Filters (GFP & RFP)
 CCD Camera and Lens:

o OptiChemi™ 610 with either 50mm f/1.2 Fixed or 28mm f/1.8 Fixed Lens
o BioChemi™ 500 with either 50mm f/1.2 Fixed or 28mm f/1.8 Fixed

 Gel Viewer
 Motorized Lift
 Epi Illumination
 BioLite MultiSpectral Source with Excitation Filters (GFP & RFP)
 Warming Plate
 VisionWorksLS Software

Check the packing slip for a complete equipment list. The system may additionally include a computer and
transilluminator.

Darkroom Cabinet

The iBox Imaging System is manufactured of aluminum construction and fabricated to provide a light-tight
chamber.

Darkroom Dimensions: 17.5W x 17.5D x 32H inches (445 x 445 x 813 mm)
Note: Camera cover dimensions additional

Emission and Excitation Filters

Green Florescent Protein (GFP) and Red Fluorescent Protein (RFP) emission and excitation filters are
included. The darkroom can accommodate five emission filters in its filter wheel. The BioLite MultiSpectral
Source is designed to accommodate up to eight excitation filters.

BioLite™ MultiSpectral Source

The BioLite Automated Multispectral Source is a 150-Watt quartz halogen visible light source designed for
use with ferrule fiber optic bundles. It features a closed optical path to tightly control the output spectrum,
allowing consistent repeatable measurements with superior signal to noise. The BioLite features a 6 position
dimmer permitting variable intensity. The integrated filter wheel accommodates up to 8 excitation filters.

Warming Plate

The warming plate houses internal heating elements which connect inside the iBox and keep the surface of
the warming plate at a constant 37°C.

CCD Camera and Lens

The CCD camera, housed in the top of darkroom, generates extremely fast optics and high sensitivity for
capturing high resolution images. The camera’s motorized lens is controlled by the VisionWorksLS software.

Gel Viewer

The viewer in the darkroom door opens for safe viewing of samples. The acrylic window allows viewing
without exposure to ultraviolet radiation.

Motorized Lift

The motorized lift, controlled by the software, can be finely adjusted within its 10 inch range.

iBox Scientia Small Animal Imaging System 4

Epi Illumination

Overhead lighting includes 365nm UV, Visi-Blue 480nm and white light. Lighting is controlled via the
software interface.

Minimum Computer Requirements

Processor: Pentium 4, 1.6 GHz or higher
RAM: 2GB (4GB Preferred)
Display: 1024 x 768
Operating System: Microsoft Windows XP Service Pack 2 or Newer (32-bit or 64-bit)
Internet Browser: Microsoft Internet Explorer 6.0 or later
Computer USB Ports: Computer must come equipped with a minimum of four USB ports; additional

peripherals (mouse, keyboard, printer, etc.) may require additional USB ports.

Anesthesia Kit (Optional)

The optional anesthesia system is an optional component designed to safely anesthetize up to five small
animals at a time inside the darkroom. The patented non-rebreathing technology safely prevents backflow of
gases into the darkroom. Anesthesia units are available with either isoflurane or sevoflurane, as well as with
or without oxygen regulators. Contact UVP for ordering information.

iBox Scientia Small Animal Imaging System 5

1. Double click the VisionWorks

2. To activate the software,

5. Complete all required information

Software Installation

Install the VisionWorks®LS Software

1. Insert the VisionWorksLS CD (not network CD) into the computer.

2. Click on the “Install” button for VisionWorksLS.

3. Click OK, Next, then agree to “I accept terms of licensing agreement”, then Next. Leave all the
defaults. Then click Next, Next, Install, and finally Finish.

Registering the Software

Open the Software

software icon on the desktop.

registration is required. To
immediately activate the software
through the internet, choose On
the fly activation. If the computer
is not connected to the internet,
please follow the instructions for
Offline activation or call UVP to
register the software.

3. Click Next to continue.

4. Already have an activat ion ID is
useful when reloading the
software after receiving an initial
activation code.

on the form.

6. Fill out the Serial Number located
on the CD. The number should be
four sets of six numbers.

7. Click onto Get Activation No. and
then click onto Activate when the
Activation Number appears in the
box.