UVP iBox Explorer Imaging Microscope User Manual

Ultra-Violet Products Ltd.
2
iBox
Explorer Imaging Microscope
®
Instruction Guide
______________________________________________________________________________
UVP, LLC
2066 W. 11th Street, Upland, CA 91786 Tel: (909) 946-3197 / (800) 452-6788 Fax: (909) 946-3597
Web Site:
Nuffield Road, Cambridge CB4 1TG UK
Tel: +44(0)1223-420022 Fax: +44(0)1223-420561
www.uvp.com
Unit 1, Trinity Hall Farm Estate
81-0342-01 Rev E
iBox Explorer2 2

Table of Contents

Table of Contents ......................................................................................................................................................... 2
Introduction .................................................................................................................................................................. 3
Safety Information ........................................................................................................................................................ 4
Components ................................................................................................................................................................. 5
Specifications ............................................................................................................................................................ 6
Darkroom Cabinet ..................................................................................................................................................... 6
Four Position Filter Tray ............................................................................................................................................ 6
CCD Camera and Objectives .................................................................................................................................... 6
Magnification and Field of View ................................................................................................................................. 6
Viewer Window .......................................................................................................................................................... 6
Motorized Sample Platform ....................................................................................................................................... 6
Warming Plate ........................................................................................................................................................... 6
VisionWorksLS Software ........................................................................................................................................... 6
BioLite Xe MultiSpectral Source ................................................................................................................................ 6
Installation .................................................................................................................................................................... 7
Installing the Software ............................................................................................................................................... 7
Registering the Software ........................................................................................................................................... 7
Installing the Hardware .............................................................................................................................................. 9
BioLite Xe MultiSpectral Light Source Setup ........................................................................................................... 10
Fiber Optic Cable Connection ................................................................................................................................. 11
Platform Stage/Warming Plate ................................................................................................................................ 12
Additional iBox Explorer
Loading Drivers ....................................................................................................................................................... 13
Operation .................................................................................................................................................................... 14
Preparing the System .............................................................................................................................................. 14
Preparing to Capture Images .................................................................................................................................. 15
Capturing Images .................................................................................................................................................... 18
Service Procedures .................................................................................................................................................... 19
Care and Cleaning .................................................................................................................................................. 19
Return Procedure .................................................................................................................................................... 19
Troubleshooting ....................................................................................................................................................... 19
Replacement Parts and Accessories ....................................................................................................................... 20
Technical Support ................................................................................................................................................... 21
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Connections .................................................................................................................... 13
iBox Explorer2 3
Introduction
The iBox Explorer2 Imaging Microscope is designed to automate research with one-touch preset or user-defined PC controls for accurate, repeatable imaging and analysis of animals and plants. The iBox Explorer tight darkroom with VisionWorks wide range of magnification settings, allowing visualization from an entire small animal down to the cellular level subcutaneously and within the body cavity of living mice.
The darkroom has a door-mounted viewing window, four-position emission filter tray with four emission filters included, and a software- and joystick-controlled imaging platform. The system comes with dual-pat h dire cte d lighti ng for exciting samples with the BioLite™ Xe MultiSpectral Light Source. The BioLite Xe offers an eight-position filter wheel, six-position intensity selector, and a direct lighting source using fiber optic bundles to tightly control the output spectrum for consistent, repeatable measurements. Two excitation filters are included, one each for the excitation of GFP (Green Fluorescent Protein) and RFP (Red Fluorescent Protein).
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The iBox Explorer stage surface at a constant 38°C. The warming plate also contains two threaded studs to accommodate a small animal anesthesi a manifold .
The iBox Explorer preview images.
Imaging Microscope comes equipped with a slide-out warming plate that maintains the platform
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includes a highly sensitive CCD camera with high magnification optics, providing real-time, live
LS Acquisition & Analysis software for automated control. The system provides a
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incorporates a light
iBox Explorer2 Imaging Microscope
iBox Explorer2 4

Safety Information

Do not use this equipment in a manner other than as stated in these instructions. This equipment is designed for use in a laboratory environment by personnel knowledgeable in safe laboratory
practices. Pro per prec autions must be taken as potential electri cal and me chan ical hazards are inherent in the use of this equipment.
CAUTION: While UVP’s warming plate is designed for the safety of users, care should be taken when operating the unit as the platfor m stage can be hot.
iBox Explorer2 5
Monochrome CCD camera and
Four position filter tray with
Access ports for
Source
Viewer window opens for
amber screen
Light tight darkroom with
wide access door
Software controlled
platform adjusts on the
Warming plate
VisionWorks®LS software
image capture & image analysis
BioLite Xe

Components

The iBox Explorer2 Imaging Microscope is comprised of the following components:
Darkroom Cabinet Joystick CCD Camera/Optics (internal and not visible) Sample Viewer Window and Internal Amber Screen Motorized Platform Slide-Out Warming Plate Emission Filters (GFP, RFP, 515 Long Pass, Neutral Density) Excitation Filters (GFP, RFP) BioLite Xe MultiSpectral Light Source VisionWorksLS Software
Please review the packing slip for a complete equipment list. System components may vary. Note: The system may also include a computer and monitor.
magnification objectives are housed inside the darkroom
BioLite Xe
MultiSpectral Light
with indicator
lights
X, Y and Z axes
RFP, GFP, 515 Long Pass and
Neutral Density filter s in clud ed
sample observation through
controls darkroom functions,
iBox Explorer2 6

Specifications

Power Requirements: 100-230V; 6.3 Amps at 120 Volts
Mains supply voltage fluctuations are not to exceed 10 percent of the nominal supply voltage
Pollution Degree: 2 Installation Category: II Altitude: Up to 2000m Ambient Temperature: 5°C to 40°C Software Requirements: Windows XP (SP2) or Windows 7 (32-bit and 64-bit versions) Humidity: Maximum relative humidity of 80% for temperatures up to 31°C, decreasing linearly
to 50% maximum relative humidity at 40°C

Darkroom Cabinet

The iBox Explorer2 is constructed of aluminum and fabricated to provide a light tight chamber. Darkroom dimensions are 17.5”W x 20”D x 42”H (44.5cm x 50.8cm x 106.7cm).

Four Position Filter Tray

The filter tray accommodates up to four removable emission filters. The iBox Explorer2 system includes RFP (Red Fluorescent Protein), GFP (Green Fluorescent Protein), 515 Long Pass and Neutral Density emission filters. VisionWorksLS software controls the selection of filters. Additional filters are available through UVP.

CCD Camera and Objectives

The high-sensitivity CCD camera and fast optics, housed in the top of darkroom, generate high resolution images. The camera and motorized optics are controlled by VisionWorksLS software.

Magnification and Field of View

The iBox Explorer2 contains a combination of relay and objective lenses, providing magnifications of 0.17x,
0.25x, 0.50x, 1.66x, 2.50x, 4.50x, 7.50x, 8.80x and 16.5x. The corresponding fields of view are 90mm2, 60mm2,
2
, 9mm2, 6mm2, 3.3mm2, 2mm2, 1.7mm2 and 0.9mm2.
30mm

Viewer Win dow

The viewer window, built into the darkroom door, opens for sample viewing.

Motorized Sample Platform

The motorized sample platform, controlled by the joystick, can be adjusted in the X, Y and Z dimensions. The platform can be moved 100mm in each direction.

Warming Plate

The warming plate creates a constant temperature of 38ºC for the warming of animal specimens. The dark background creates an ideal imaging surface while maintaining a constant temperature for the animal.

VisionWorksLS Software

The VisionWorksLS Acquisition & Analysis software controls the darkroom functions and lighting as well as the motorized optics and camera. T he softwar e is operational on both 32-bit and 64-bit Windows operating systems.

BioLite Xe MultiSpectral Source

The BioLite Xe MultiSpectral Source provides dual path excitation lighting for specimens. GFP and RFP filters come standard with the BioLite Xe, and custom filters for specif ic appl ic ations are also available. Refer to “Replacement Parts and Accessor ies” for orderi ng information.
iBox Explorer2 7

Installation

Installing the Software

Insert VisionWorksLS CD (not network CD) into the computer. Click on the Install button for VisionWorksLS. Click OK, Next, agree to “I accept terms of licensing agreement”, then Next. Leave all options in
their default settings. Then click Next, Next, Install and finally Finish.

Registering the Software

Open the Software
Double click VisionWorksLS software icon on the desktop.
To activate the software, registration is
required. To immediately activate the software online, choose On-the-Fly activation. If the computer is not connected to the Internet, select Offline activation and proceed to the following page of this manual, or call UVP to register the software.
Click Next to continue. The Already have an activation ID option
is useful when reloading the software after receiving an initial activation code.
Complete all required information on the form.
Fill out the Serial Number located on the
CD. The number should be four sets of six numbers.
Once the form is completed, click on Get
Activation No. and then click Activate
once the Activation Number appears in the box.
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