UVP iBox Anesthesia System User Manual

®

iBox

Anesthesia System

Instruction Manual

UVP, LLC Ultra-Violet Products Ltd.
2066 W 11th Street, Upland, CA 91786 Unit 1, Trinity Hall Farm Estate,
Tel: [phone] Nuffield Road Cambridge CB4 1TG UK
Fax: [phone] Tel: [phone] / Fax: [phone]

Web site: www.uvp.com

81-0303-01 Rev H

iBox Anesthesia System 2

IMPORTANT!

Read this manual before setting up and operating the iBox Anesthesia System!

The user should be thoroughly familiar with the contents of this manual prior to using the system with
animals.

Only technicians who are properly certified to use isoflurane/sevoflurane vaporizer and anesthetic
equipment should operate the iBox Anesthesia System.

The user/owner of this equipment shall have the sole responsibility for any damage or injury resulting from
operation that is not in accordance with the authorized instructions. This includes, but is not limited to,
operating the equipment outside of recommended safety levels, variation from specified operating
instructions and not following standard laboratory safety procedures when working with anesthetic agents
and volatile compressed gases.

The system and its components must only be modified or repaired by factory authorized ser v ice techn ici an s.
Improper modification or repair may result in danger to personnel, harm or death to animal, or equipment
damage. The user/owner of this equipment shall have the sole responsibility for any damage or injury
resulting from improper maintenance and repair that is not performed by authorized maintenance and repair
personnel.

Components that have failed in whole or in part, exhibit excessive wear, are contaminated or are otherwise
at the end of their useful life, should not be used. To ensure proper system operation, use only UVP­supplied parts for repair or replacement.

Opening the vaporizer unit by unauthorized personnel automatically voids all warranti es an d specif icat io ns.
The manufacturer assumes no responsibility for any malfunction or failure of the unit if the seal is broken.

iBox Anesthesia System 3

Introduction to the iBox Anesthesia System

The iBox Anesthesia System is designed for the anesthesia of small animals. The system is a small
portable unit that has been designed for maximum efficiency, ease of use and low anesthetic gas
consumption.

The system delivers a precisely blended mixture of oxygen and isoflurane or sevoflurane into a
plastic induction chamber, offering ultra-efficient anesthetizing of animals. The chamber attaches to
a charcoal filter canister that releases safe, filtered air into the room.

After initial anesthetizing in the host cage, the animal can be moved to the breathing
device/manifold attached to the warming plate inside the iBox Imaging System. The non­rebreathing gas delivery masks, or “nosecones,” are specifically designed for small animals. The
system allows safe controlled anesthesia during imaging procedures to keep the animal free of
movement.

Note that two styles of anesthesia kits are offered for use with UVP’s iBox Imaging System line: the
five-port and the single-port breathing device/manifold. The five-port kit is designed for use with the
iBox Scientia™ and iBox Spectra™ Small Animal Imaging Systems, while the single-port kit is
designed for the iBox Explorer™ Imaging Microscope. Most of the components are the same
between the two anesthesia kits, except for a few notable differences:

1. The five-port is able to accommodate up to five mice simultaneously, while the single-port
accommodates a single mouse.

2. The warming plate for the five-port kit is removable from the darkroom along with the
nosecone manifold, while the single-port’s warming plate is a slide-out unit attached to the
inside of the darkroom. Thus, only the single-port nosecone manifold and bracket are
removable from the darkroom.

NOTE: Anesthesia kits are designed to support the use of either isoflurane or sevoflurane agents
for anesthetization. Note that each kit is designed to accommodate either isoflurane or
sevoflurane, not both. DO NOT use one agent in a system designed for the other, as permanent
system damage will occur. Read the label on the system to determine which type of agent it is
designed to utilize.

iBox Anesthesia System 4

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Components of the iBox Anesthesia System

1) Five-Port Breathing Device/Manifold: The Five-Port Breathing Device/Manifold is designed
to maintain anesthesia in the iBox Scientia and iBox Spectra for up to five mice or one rat at a
time. The inflow tube delivers anesthet ic gas from the vaporizer unit to the nosecones, while
the outflow tube directs expended gas from the nosecones to the charcoal fi lter ca nis ter. Each
breather is specifically designed to accom mod ate cert a in ani mal s. The five-port breather will
accommodate animals weighing 0 to 500 grams.

Not Pictured: The Single-Port Breathing Device/Manifold, for use in the iBox Explorer,
attaches via thumb screws to the slide-out warming plate built into the iBox Explorer. It is
capable of maintaining anesthesia for a single mouse at a time.

2) Nosecones and Plugs: The interchangeable species-specific nosecones slip onto the collar
on each port of the breathing device/manifold. The black plugs (included) prevent release of
anesthetic gas and are to remain in the nosecones when not in use.

3) Warming Plate: In the iBox Scientia and iBox Spectra, the five-port breathing device/manifold
connects to the warming plate. Heating elements within the warming plate keep the surface of
the plate at a consistent 37°C.

Not Pictured: In the iBox Explorer, the single-port breathing dev ice/manifold connects to the
slide-out warming plate that is built into the system. Heating elements within the warming plate
keep the surface of the plate at a consistent 37°C.

4) Oxygen Regulator: The oxygen regulator, with a factory preset pressure output of 50 psi,
attaches to an industrial-grade oxygen tank with a standard CGA-540 tank connection. The
regulator’s output fitting is an oxygen-specific DISS 1240 male connection and is also available
with a CGA-870 yoke tank connection for medical-style oxygen tanks.

NOTE: Depending upon configuration, this system may or may not contain an oxygen
regulator. An alternate oxygen regulator can be used only if it has an oxygen-specific
DISS 1240 male connection and is capable of regulating pressure from 20-70 psi.
Recommended output pressure to the system is 50 psi. If your system was not configured
with an oxygen regulator, contact a lab supply company for more information.

UVP does not ship oxygen with this system. Contact a lab supply company to purchase
oxygen.

iBox Anesthesia System 5

5) Isoflurane/Sevoflurane Vaporizer: The vaporizer is a precision instrument that blends
oxygen and either isoflurane or sevoflurane to the proper ratio, and then outputs it to the
system components. The gas blend is adjusted with a large locking dial on top of the unit. The
vaporizer outputs agent concentrations from 0-5% in .5% graduati ons .

NOTE: UVP does not ship isoflurane or sevoflurane with thi s system . Contact a lab supply
company to purchase isoflurane or sevoflurane.

6) Vaporizer Stand: The vaporizer stand acts as a mount for the vaporizer and the gas delivery
mechanisms, including the inlet flow meter and the on/off valves for the breathing device and
chamber. Quick disconnects are used to attach the breathing device and chamber to the unit.

7) Induction Chamber: The induction chamber is a small acrylic plastic chamber designed to
anesthetize up to 5-6 mice or one rat at a time. The chamber includes a quick disconnect gas
inlet fitting and an exhaust outlet to a charcoal filter canister.

8) Charcoal Filter Canisters and Stand: The ReFresh charcoal filter canisters use activated
charcoal to remove isoflurane or sevoflurane from waste gas before release into the room. A
filter is installed on the chamber and breather ex haust. Filter s are to be weighed prior to initial
use and prior to each subsequent use. The charcoal filter canister should be replaced after

a weight increase of 100 grams.

iBox Anesthesia System 6

Oxygen Tank

Setup of iBox Anesthesia System

The following is a schematic diagram of the five-port iBox Scientia and iBox Spectra anesthesia system. The specific components (letters) of this schematic will be referenced parenthetically throughout this manual.

Note: The only difference between this schematic and the anesthesia system for the iBox Explorer is the replacement of a single-port breathing device/manifold in place of the five-port component shown below.

Connecting Outside the Darkroom

1. Attach the Regulator to the Oxygen Tank (Figure 1):
Be sure that the oxygen tank is secured and stable

before attaching the regulator.

 To mount the oxygen regulator (B) to the oxygen

tank, attach regulator fitting to the oxygen tank
output and tighten the black handle wheel.

Figure 1: Attach Regulator to