UVP HybriCycler User Manual

HybriCycler Hybridization Oven

Operating Instructions

IMPORTANT: Please read these instructions before operating your UVP HybriCyclerTM

system to familiarize yourself with operation procedures.

81-0187-01 Rev C

HC-3000 HybriCycler Hybridization Oven 2

Introduction

The HybriCycler Hybridization Oven is designed as a compact alternative for
hybridization requirements. The HybriCycler Oven features microprocessor operation for
precise mixing capability. This space-saving design is manufactured with a 3/8” thick
beta-blocking acrylic and polycarbonate cylinder providing 360° view of the bottles. The
HybriCycler Oven housing is manufactured with a scratch-resistant, powder-painted
epoxy finish for easy cleaning and maintenance.

The HybriCycler operates at 11 RPM, enabling consistent saturation of samples,
whether is for washing or hybridizing. Having the ability to remove the carousel allows
for additional bottle size capacity. An easily removable protective tray under the rocker
wheel allows easy clean-up of spilled media.

A touch sensitive keypad and microprocessor control accurate temperature output from
ambient +10
desired hybridization temperature is entered, the readout displays the current
temperature inside the chamber. The HybriCycler Oven features:

o

C to 80oC. The keypad is located just below a large LED readout. Once the

Temperature Display

Power Controls

Carousel Chamber

Bottle Carousel

Spill Tray

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HybriCycler Hybridization Oven Hardware

The HybriCycler Oven is comprised of:

Hybridization Chamber

The space-saving Hybridization Chamber
is manufactured of 3/8” thick beta-blocking
acrylic providing a 360° view of the bottles.

Bottle Carousel

The cartridge-style bottle carousel is
manufactured of ¼” thick polycarbonate.
The carousel has the capacity to hold

• Four 35x300mm bottles

• Eight 35x150mm bottles

• Eight 50ml bottles

or one oversize bottle 70x300mm by
removing the carousel.

Spill Tray

The drawer-style spill tray is designed to
contain spills that leak from any of the
bottles within the Hybridization Chamber.
The spill tray has a fluid capacity of 300ml.

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Set-Up Instructions

This equipment is not intended for interconnection with any other devices. Use of this equipment
other than intended may create a safety hazard and/or malfunction.

1. Plug the female end of the power cord

to the connection on the back of the
HybriCycler.

2. Insert your Hybridization bottle(s) into

the bottle carousel. Start by sliding the
bottle into the bottle carousel’s entry
hole at either end of the bottle
carousel.

WARNING: Do not insert a bottle into the
center tube. To do so would block air flow
and may result in a safety hazard and or
malfunction.

3.

When the end of the hybridization
bottle is about to meet the bottle
carousel’s exit hole on the opposite
end, push the bottle down toward the
center of the bottle carousel, which
secures the bottle into the securing
rings. Then slide the hybridization
bottle through the bottle carousel’s exit
hole.

4. Open the HybriCycler’s door by

pressing down on the rear portion of
spring-loaded door latch. Slide the
bottle carousel into the hybridization
chamber ensuring that the front disk is
inserted into the groove of the carousel
guide. Close the door by squeezing the
door against the hybridization chamber
until the spring-loaded door latch
secures.

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Operation

HybriCycler functions:

• The “Power” switch turns the
HybriCycler ON or OFF.

• The “Motor” switch turns the bottle
rotation ON or OFF.

• The “Temperature Control” displays
and controls the temperature
setting within the hybridization
chamber.

1. To operate the unit, press the “Power”
switch to the ON position.

2. Set the “Temperature Control” to the
required temperature by pressing
either the “Up” or “Down” keypad arrow
buttons to raise or lower the displayed
degree (displayed in degrees Celcius).
This setting is adjustable from ambient

o

+10

C to 80.0°C. See Temperature
Control Instructions on page 6 for more
details.