UVP HM-4000 User Manual

Hybridizer, HybriLinker and

Ultra-Violet Products Ltd.

Multidizer Hybridization Ovens

Operating Instructions

HB-1000 Hybridizer HM-4000 Multidiz er HL-2000 HybriLinker

IMPORTANT: To familiarize yourself with the correct operation procedures, read these

instructions before operating your UVP Hybridizer, Multidizer or HybriLinker system.

_____________________________________________________________________________________

UVP, LLC

2066 W. 11th Street, Upland, CA 91786
Tel: [phone]
Fax: [phone]

Web Site:

Nuffield Road, Cambridge CB4 1TG UK

Tel: [phone] Fax: [phone]

www.uvp.com

Unit 1, Trinity Hall Farm Estate

81-0169-01 Rev T

Hybridization Ovens

Table of Contents

Table of Contents ........................................................................................... 2

Introduction ..................................................................................................... 3

Hybridization Oven Spe c ifications .................................................................. 4

Setup Instructions ........................................................................................... 6

Operating Instructions .................................................................................... 8

Care and Cleaning ........................................................................................ 13

Replacement Parts ....................................................................................... 14

Accessories .................................................................................................. 16

Maintenance, Repair an d Technical Assistance ........................................... 17

Hybridization Techniques ............................................................................. 18

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Hybridization Ovens

Introduction

The HB-1000 Hybridizer is a cost-effective, high-performance, 10-bottle hybridization oven with state-of-the-art
microprocessor temperature a nd variable speed controls. The HL-2000 HybriLinker™ System is designed with a
combination of hybridizer and crosslinker components. This equipment is intended to be used in the laboratory
environment.

The HM-4000 Multidizer™ System is designed as a multifunction unit with a variety of motion options. The lower
chamber operates in the same manner as the HB-1000. However, the HM-4000 has an additional upper chamber for
the use of a reciprocating shaker tray, an orbital rocker tray, or an acrylic carousel. Refer to the “Accessories” section
of this manual for ordering information.

The HB-1000 Hybridizer can be set to operate anywhere from 10 to 15 RPM, enabling consistent saturation of
samples for washing or hybridizing. Having the ability to remove the rotary carousel wheel and replace it with an
interchangeable rocker plate enables all hybridization protocols in one reliable benchtop unit. An easily removable
protective tray under the carousel allows easy clean-up of spilled media.

A touch-sensitive keypad and micropro ce ssor prov ide accurate temperature control from ambient 10°C to 99.9°C.
The keypad is located just below a large LED readout. Once the desired hybridization temperature is entered, the
readout displays the current temperature inside the chamber. The chamber environment is calibrated at 68.0°C. At
this temperature, the accuracy is +/-0.5°C. An internal circulating fan assists in uniformly distributing heating
throughout the chamber.

Two different hybridizer bottle sizes are available for use within the hybridizer using the standard carousel provided
with the unit. The rotary carousel wheel can hold up to twenty (20) 35 x 150 mm bottles or ten (10) 35 x 300 mm
bottles. In addition, combinations of both size bottles may be used at the same time. An op ti onal carousel kit for
oversized bottles is available.

There may be build up of pressure within the hybridization bottles when they are taken from ambient to hybridization
temperature. To help in relieving some of this pressure, preheat the solutions and bottles. Also, be sure bottles are
opened at the same temperature at which the hybridization was done. Do not allow the bottles to cool before opening.

To ensure the hybridization bottles remain leak proof and pressure proof, prevent temperatures above 70°C. If
temperatures above this are used without first relieving the pressure within the bottle, there is a risk that bottles will
leak and/or break due to internal pressure build-up.

If the bottles are accidentally taken above 70°C without relieving the pressure, DO NOT open the hybridizer door.
Turn off the hybridizer and allow it to cool before opening the door and checking the contents of the oven. Should the
bottles be used above 70°C, relieve the pressure by unscrewing and re-t ight ening the cap.

If using Radioactive material, see the “Decontamination” section of this manual.

The HL-2000 HybriLinker System and HM-4000 Multidizer System are designed to be carried or lifted by two
people.

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Hybridization Ovens

Hybridization Oven Specifications

HB-1000 Hybridizer Hybridization Oven

Part Numbers Volts/Hz

95-0030-01 115V/60Hz
95-0030-02 230V/50Hz
95-0030-03 100V/50Hz

Specifications

Net Weight: 43 lbs
Temperature: Ambient +10°C to 99.9°C
Temperature Stability: Inside Oven: +/-0.3°C to 68°C

Inside Bottles: +/-0.1°C to 68°C

Temperature Uniformity: Inside Oven: +/-0.1°C to 68°C

Inside Bottles: +/-0.1°C to 68°C
Temperature Display: LCD
Heating Element: 1250 watts
Rotation Speed: Off, 10 to 15 RPM
Bottle Capacity: Twenty (20) 35 x 150 mm, Ten (10) 35 x 300 mm
Dimensions: Exterior 17.5W x 16H x 16D in. (445 x 406 x 406mm)

Interior 14W x 10.75H x 10.75D in. (356 x 273 x 273mm)

HL-2000 HybriLinker System

The HybriLinker System combines a hybridization oven and a crosslinker in one space-saving unit. Each
section has separate power supplies for independent operation.

Part Numbers Volts/Hz

95-0031-01 115V/60Hz
95-0031-02 230V/50Hz
95-0031-03 100V/50Hz

Specifications

Unit Net Weight: 60 lbs
Dimensions: Exterior 17.5W x 24H x 15D in. (445 x 610 x 381mm)

Hybridization Oven

Temperature: Ambient +10°C to 99.9°C
Temperature Stability: Inside Oven: +/-0.3°C to 68°C

Inside Bottles: +/-0.1°C to 68°C
Temperature Uniformity: Inside Oven: +/-0.1°C to 68°C

Inside Bottles: +/-0.1°C to 68°C
Temperature Display: LCD
Heating Element: 1250 watts
Rotation Speed: Off, 10 to 15 RPM
Bottle Capacity: Twenty (20) 35 x 150 mm, Ten (10) 35 x 300 mm
Interior Dimensions: 14"W x 10.75"H x 10.75D (356 x 273 x 273mm)

Crosslinker

Microprocessor controls 8 watt Shortwave UV (254nm) tubes
Large LED displaying time or energy Tactile membrane keypad
Preset or user-selected UV time exposure settings Internal interlocking safety system
Preset or user-selected UV energy settings

Maximum UV energy setting: 999,900 microjoules per cm
Interior Dimensions: 11.375W x 3.75H x 11.5D in. (288 x 95 x 292 mm)

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Hybridization Ovens

HM-4000 Multidizer Hybridization Oven

Part Numbers Volts/Hz

95-0340-01 115V/60Hz
95-0340-02 230V/50Hz
95-0340-03 100V/50Hz

Specifications

Net Weight: 75 lbs (34 kg)
Temperature Display: LCD
Dimensions: Exterior 17.5W x 28.5H x 15D in. (445 x 724 x 381 mm)

Upper Chamber

Temperature: Ambient +10°C to 80°C
Temperature Stability: Inside Oven: +/-0.3°C to 68°C

Inside Bottles: +/-0.1°C to 68°C
Temperature Uniformity: Inside Oven: +/-0.1°C to 68°C

Inside Bottles: +/-0.1°C to 68°C
Heating Element: 500 Watts
Shaker Tray Speed: 54 - 106 cycles/min.
Carousel Rotation: 12 - 20 rpm

Lower Chamber

Same as the HB-1000 Hybridizer

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Hybridization Ovens

Fig. 2 Adjusting for Bottle Lengths

Wheel

Rod

Thumb

Screw

Fig. 1 Inserting Bottle Holder Carousel

Fig. 3 Inserting Bottles

Fig. 4 Offset/Skewed Bottle Positioning

Setup Instructions

Two different bottle sizes are available for use within the hybridizer. The rotary wheel carousel can hold up to twenty
(20) 35 x 150 mm bottles or ten (10) 35 x 300 mm bottles. In addition, combinations of both bottle sizes may be used
at the same time.

• To insert bottle holder carousel (Fig. 1), slide the two wheels onto the rod and tighten the thumb screws (Fig.

2). Insert the rod into the holder on the right wall of the hybridizer chamber. Then, slide the rod into the
holder on the left wall of the chamber.

• To adjust for various bottle lengths, loosen the thumb screws on the bottle clip wheel and adjus t to desir ed
width.

• To insert bottles into bottle clips, gently push the bottle into the clips (Fig. 3). Insert one side at a time for
large bottles.

• If offset/skewed bottle positioning is desired (Fig. 4), loosen the thumb screw for only one bottle clip wheel.
Slightly rotate the wheel until the required angle is obtained. Retighten the thumb screw. (Note: Offset or
skewed bottle positioning can allow for better mixture of bottle contents.)

NOTE: ALWAYS USE AN EVEN NUMBER OF BOTTLES AND LOAD BOTTLES OPPOSITE EACH OTHER TO
BALANCE THE BOTTLE HOLDER. THIS PREVENTS EXCESSIVE WEAR OF THE BEARINGS AND DRIVE
MOTOR.

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Hybridization Ovens

Reciprocating Shaker Tray

Rocker Tray

Orbital Motion Tray

Installing the Rollers and Optional Trays

To insert rollers in the hybridization oven carousel, slide the rollers into the receptacle on the left side of the chamber
first, followed by the right side.

To install the Reciprocating Shaker Tray (standard with the HM-4000), place the tray over the rollers in the
hybridization oven.

To install the Rocker Tray (optional on all units), remove the metal carousel from the chamber. Place the tray into the
chamber with the coupling arm to the right side. Place the coupling sleeve over the motor drive shaft such that the
pins on the inside of the coupling sleeve engage the slots in the motor drive shaft of the chamber. Rotate the sleeve
counter-clockwise 30° until locked in place. To remove the tray, reverse the process.

To install the Orbital Motion Tray (optional on all units), open the chamber door, remove the carousel, connect the
power cable to the orbital tray and to the power port inside the unit, and place the tray in the oven so the switch is
accessible. Turn the hybridization oven Motor and Power switches ON, and turn the orbital tray Power switch ON.

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