UVP High Performance UV Transilluminators User Manual

High Performance
UV Transilluminators

Instruction Manual

UVP, LLC Ultra-Violet Products Ltd.
2066 W 11th Street, Upland, CA 91786 Unit 1,Trinity Hall Farm Estate,
Tel: [phone] Nuffield Road Cambridge CB4 1TG UK
Fax: [phone] Tel: [phone] / Fax: [phone]

Web site: www.uvp.com

81-0278-01 Rev C

High Performance UV Transilluminators Page 2

UV Blocking Cover is
Models include a control

Power switch

UVP’s long life filters are
filter sizes

Introduction

The UVP Transilluminator offers the researcher uniform and intense sources of ultraviolet light
(radiation) in a compact package. The special design emits high intensity excitation UV wavelength
for back-illumination of transparent fluorescent materials. The 302nm UV back-illumination provides
a highly sensitive method to detect double-stranded nucleic acids that have been labeled with
fluorescent dyes such as ethidium bromide or acridine orange. Single stranded nucleic acids may
be detected, but with a lower excitation wavelength more sensitive for nucleic acid visualization
than the 365nm model.

High Performance UV Transilluminators are fitted with the exclusive 25 watt UV tubes. The
transilluminator is uniquely designed with increased UV intensity and uniformity, instant on
capabilities, no lamp flicker and reduced electrical consumption. Select models include variable
intensity with High, Medium and Low settings. The electronic ballast has a universal voltage
capabilities.

NOTE: Though the manual refers to the midrange UV waveband as 302nm, others refer to this
region as 300nm or 312nm. The spectral output of all these regions is the same.

A Word of Caution: UV Transilluminators are powerful sources of UV radiation that will cause
damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area
are properly protected. If not using the transilluminator with an imaging system darkroom, a UV
Blocking Cover should be attached to the transilluminator. Even though this cover blocks the
ultraviolet radiation emitted by the unit, UV Blocking Eyewear should be worn as well.

UVP UV Transilluminator features:

included with the
transilluminator. The cover
is hinged and adjustable to
varying heights

knob for selection of
wavelength or intensity

available in a variety of

High Performance UV Transilluminators Page 3

Specifications

Single UV High Performance Transilluminators

Single UV High Performance Transilluminators are designed with variable intensity settings.
Units are equipped with an electronic ballast and 25 watt tubes.

Width: 19.13” (486mm) Depth: 13.25” (336mm) Height: 5.63” (143mm) Height includes cover

The following models have 302nm midrange UV:

Part Wave- No. of Intensity
Model Number length Volts/Hz Filter Size Tubes Style

TFM-20V 95-0423-01 302nm 100-115V/60Hz 20 x 20 cm 4 x 25W Variable
TFM-20V 95-0423-02 302nm 230V/50Hz 20 x 20 cm 4 x 25W Variable
TFM-26V 95-0422-01 302nm 100-115V/60Hz 21 x 26 cm 4 x 25W Variable
TFM-26V 95-0422-02 302nm 230V/50Hz 21 x 26 cm 4 x 25W Variable
TFM-30V 95-0424-01 302nm 100-115V/60Hz 25 x 30 cm 4 x 25W Variable
TFM-30V 95-0424-02 302nm 230V/50Hz 25 x 30 cm 4 x 25W Variable
TFM-40V 95-0421-01 302nm 100-115V/60Hz 20 x 40 cm 4 x 25W Variable
TFM-40V 95-0421-02 302nm 230V/50Hz 20 x 40 cm 4 x 25W Variable

The following models have 365nm longwave UV:

TFL-40V 95-0420-01 365nm 100-115V/60Hz 20 x 40 cm 4 x 25W Variable
TFL-40V 95-0420-02 365nm 230V/50Hz 20 x 40 cm 4 x 25W Variable

The following models have 254nm shortwave UV:

Part Wave- No. of Intensity
Model Number length Volts/Hz Filter Size Tubes Style

TFS-20V 95-0427-01 254nm 100-115V/60Hz 20 x 20 cm 4 x 25W Variable
TFS-20V 95-0427-02 254nm 230V/50Hz 20 x 20 cm 4 x 25W Variable
TFS-26V 95-0428-01 254nm 100-115V/60Hz 21 x 26 cm 4 x 25W Variable
TFS-26V 95-0428-02 254nm 230V/50Hz 21 x 26 cm 4 x 25W Variable
TFS-30V 95-0429-01 254nm 100-115V/60Hz 25 x 30 cm 4 x 25W Variable
TFS-30V 95-0429-02 254nm 230V/50Hz 25 x 30 cm 4 x 25W Variable
TFS-40V 95-0430-01 254nm 100-115V/60Hz 20 x 40 cm 4 x 25W Variable
TFS-40V 95-0430-02 254nm 230V/50Hz 20 x 40 cm 4 x 25W Variable

High Performance UV Transilluminators Page 4

When the UV Blocking Cover is not being
used, UV light may escape through the
holes dedicated to accepting the bracket

Safety Plug

2UV High Performance Transilluminators

Units are equipped with an electronic ballast and 25 watt tubes. Four tubes of each wavelength are
installed. These models have a single intensity. The physical dimensions of all models are:

Width: 14” x Depth: 11” x Height: 4.8” (356 x 279 x 122mm)

Part Wave- No. of Intensity
Model Number length Volts/Hz Filter Size Tubes Style

TFML-20 95-0431-01 302/365nm 100-115V/60Hz 20 x 20 cm 4 x 25W Single
TFML-20 95-0431-02 302/365nm 230V/50Hz 20 x 20 cm 4 x 25W Single
TFML-26 95-0425-01 302/365nm 100-115V/60Hz 21 x 26 cm 4 x 25W Single
TFML-26 95-0425-02 302/365nm 230V/50Hz 21 x 26 cm 4 x 25W Single
TFML-30 95-0432-01 302/365nm 100-115V/60Hz 25 x 30 cm 4 x 25W Single
TFML-30 95-0432-02 302/365nm 230V/50Hz 25 x 30 cm 4 x 25W Single
TFML-40 95-0436-01 302/365nm 100-115V/60Hz 20 x 40 cm 4 x 25W Single
TFML-40 95-0436-02 302/365nm 230V/50Hz 20 x 40 cm 4 x 25W Single

Transilluminator Operation

Safety Precautions

pins of the UV Blocking Cover.

 Remove the black safety plugs from

their package

 Insert the safety plugs thr ough the

holes as shown.

Set-Up

 Place the transilluminator on a l evel work surface. Be sure that an air space exists

around the bottom of the work surface. This space allows for the proper air
circulation through the unit.

 Plug the female end of the power cord into the transilluminator. For 230 volt

models, or those requiri ng special power cord connector s, ensure that the proper
configuration of male connector or plug is properly connected to the power cord.

 Plug the male end of the p o wer cor d int o a pr o perly grounded electric al outlet. The

proper voltage of the transillum inator is found on the product information label. If
using the transillum inator with an imaging system, a j umper cable is required for
connecting to the darkroom. Refer to the imaging system documentation for
additional instructions.

 The transilluminator may be equipped with a UV Blocking Cover. Remove the

brown protective pa per fr om the cover. Ins ert t he brack et pins o n the c over in to the

High Performance UV Transilluminators Page 5

holes on the front of the transil luminator. The cover is adjus table to varying angles
for access to the filter surface.

If you are not using the tra nsilluminator with an imaging s ystem darkroom, do not
operate the unit w ithout securing the cover. If the co ver is missing, a U V Blocking
Faceshield must be worn to avoid UV exp osure to the skin. UV Blocking Eyewear
should be worn even with the cover in place to avoid accidental UV exposure.

Using the Transilluminator

Place gel/sample on the f ilter area . It is r ecomm ended to pl ace the ge ls on a Gel-Tray to
protect the f ilter sur face f rom cuts and scratc hes. It is recomm ended that gloves be worn
to avoid contact with gel and staining agents.

Press the ON/OFF switc h t o ON . The UV tubes within the un it should be glowing beneath
the filter. If us ing the tr ansil lum inator with a n im aging s ystem , the s ystem ’s main po wer is
required to be in the ON position.

When using a transilluminator with multiple UV wavelengths, dial the knob to the
appropriate wavelength setting.

When using the Variable Intensity models, use the variable intensity settings as follows:

 High: allows for UV excitation of fluorophores on gels for routine photography

and for excitation of gels with low sample concentration

 Medium: Excellent for viewing and quick single-band excision
 Low: Allows for positioning an d preparation of gels, exc ising multiple bands and

focusing for photography

After viewing/photographing the sample, turn the transilluminator off.

Cleaning and Care of the Transilluminator

Clean unit surface with a damp soft cloth or sponge. Never use abrasive cleaners (can
damage the UV filter surface).

To protect the filter glass and minimize moisture and liquids on the glass, it is
recommended that you use a UV transmitting Gel-Tray. Refer to the Replacement Parts
for ordering inform ation.

Replacing Tubes in the Transilluminator

1. Disconnect the transilluminator for the electrical supply.

2. Remove the filter cover: Use a Phillips head screwdriver to remove the four screws
on the sides of the unit. Lift the filter cover off the unit.

3. Remove the reflectors on the left and right side of the unit: Slide the reflectors up out
of the unit.

4. Remove the tube: Carefully rotate the tube and slide out of the socket. Replace with
a new tube by sliding the tube into the socket and rotating into place.

5. Insert the reflectors back into place and reattach the filter cover.

High Performance UV Transilluminators Page 6

Maintenance/Repair/Technical Assistance

Replacement Parts/Accessories

For replacement parts or components not shown here, contact UVP Cust omer Servi ce or plac e of
purchase. Please have the transilluminator model number available when you call.

Replacement Part Description Part Number

Filter Assembly (TFM-20V, TFS-20V, TFML-20) 38-0190-02
Filter Assembly (TFM-26V, TFS-26V, TFML-26) 38-0190-09
Filter Assembly (TFM-30V, TFS-30V, TFML-30) 38-0190-11
Filter Assembly (TFM-40V, TFS-40V, TFL-40V) 38-0190-10
Filter Assembly (TFML-40) 38-0190-04
Cover, UV blocking 19-0112-01
Tube, 25 Watt, 302nm midrange UV 34-0072-01 (Qty. 4 Required)
Tube, 25 Watt, 365nm longwave UV 34-0060-01 (Qty. 4 Required)
Tube, 25 Watt, 254nm shortwav e UV 34-0073-01 (Qty. 4 Required)
Ballast, electronic, 2UV models 42-0068-03 (115V)
42-0068-05 (230V)
Ballast, electronic, variable intensity models 42-0067-03 (115V)
42-0067-05 (230V)
Fuse, 3.2A, 250V, Slo-Blo 56-0002-05 (Qty. 2 Required)
Fuseholder, 5 x 20mm, RoHS 56-0023-02 (Qty. 2 Required)
Switch 53-0126-03

Accessories Description Part Number

Gel-Cutter 85-0002-01
Gel-Scooper 85-0006-01
Gel-Tray, UV Transmitting, Sm. 11.5”W x 9”D (29 x 23cm) 85-0007-01
Gel-Tray, UV Transmitting, Lg. 16.5”W x 10.5”D (42 x 27cm) 85-0005-01
Gel-Tray, High Performance UV Transmitting (25 x 26cm) 38-0296-03
Gel-Ruler, UV Fluorescing 85-0003-01
Spectacles, UV Blocking 98-0002-01
Goggles, UV Blocking 98-0002-02
Faceshield, UV Blocking 98-0002-04

High Performance UV Transilluminators Page 7

days, between 7:00 am and 5:00 pm, PST.

days between 8:30 am and 5:30 pm.

Contacting Technical Support

UVP offers expert technical support on all of our products. If you have any questions about the product’s use,
operation or repair, please contact our offices at the locations below.

Note: A Returned Goods Authorization (RGA) number must be obtained from UVP’s Customer Service prior to
returning any product.

If you are in North America, South America,
East Asia or Australia:

Call [phone] or [phone], and ask
for Technical Support during regula r busin es s

If you are in Europe, Africa, the Middle East
or Western Asia:

Call [phone], an d ask for
Customer Service during regular business

E-mail your message to: [e-mail] or

[e-mail]

Fax Technical Support at [phone]

Write to: UVP, LLC 2066 W. 11th Street, Upland,

CA 91786 USA

E-mail your message to: [e-mail]
Fax Customer Service at

[phone]
Write to: Ultra-Violet Products Ltd. Unit 1,

Trinity Hall Far Estate, Nuffield Road,
Cambridge CB4 1TG UK

Warranty

UVP, LLC warrants its Ultraviolet Transilluminators to be free of detects in materials and workmanship
for a period of two (2) years from date of purchase. The foregoing warranty of UVP shall be of no force
and effect if buyer has modified or damaged the product. Tubes and filters are warranted for 90 days.

All warranties or merchantability and fitness for any purpose and all other warranties, expressed or
implied, except those expressly set forth herein, are deemed waived and excluded.

UVP’s duty under the warranty is limited to replacement and/or repair of the defective part at the option
of UVP, LLC. UVP shall not be liable for any expenses or damages incurred by the purchaser except
as expressly set forth herein, and in no event shall UVP be liable for any special, incidental or
consequential damages of any kind. This warranty does not supersede any statutory rights that may be
available in certain countries.

UVP … Providing Quality Products for the Researcher Since 1932

From its start in 1932, UVP has become a story of growth and value fulfillment. Today, UVP services the needs
of science, industry and education throughout the world. A special significance of UVP research and
development arises from our constant communication with customers. UVP continually invites your comments
toward the improvement of UVP products or the development of custom types of ultraviolet light sources. UVP
will work with you through development and production of a product that meets your specific needs. From all of
us at UVP, Thank You.