UVP High Performance Ultraviolet Transilluminators User Manual

Replacement Parts and Accessories

For replacement parts or components not shown here, call UVP Customer Service or place of purchase.
Please have the transilluminator model number available when you call.

Operating Instructions

High Performance Ultraviolet Transilluminators

Replacement Parts Accessories

Part Number Description Part Number Description
34-0060-01 Tube, 25 watt, 365nm longwave 85-0002-01 Gel-Cutter

34-0072-01 Tube, 25 watt, 302nm midrange 85-0006-01 Gel-Scooper
38-0189-10 Filter, Assembly for TFL-40 models 85-0005-01 Gel-Tray, UV Transmitting, Large
38-0189-02 Filter, Assembly for TFM-20 models 85-0003-01 Gel-Ruler, UV Fluorescing
38-0189-09 Filter, Assembly for TFM-26 models 98-0002-01 Spectacles, UV Blocking
38-0189-04 Filter, Assembly for TFM-40 models 98-0002-02 Goggles, UV Blocking
42-0054-01 Ballast, 115V models 98-0002-04 Faceshield, UV Blocking
42-0054-02 Ballast, 230V models 97-0015-02 UVX Radiometer
42-0054-03 Ballast, 100V models 97-0016-04 UVX-31 Sensor, 302nm
56-0002-05 Fuse, 3.2 AMP, Slo-Blo 97-0016-02 UVX-35 Sensor, 365nm

Note: The UV transmitting Gel-Tray protects the transilluminator filter glass from scratches.

Warranty

UVP, LLC warrants its ultraviolet transilluminators to be free of defects in material and workmanship for a period of two (2) years
from the date of purchase. The foregoing warranty of UVP shall be of no force and effect if buyer has modified or damaged the
product. Tubes and filters are warranted for 90 days.
All warranties or merchantability and fitness for any purpose and all other warranties, expressed or implied, except those
expressly set forth herein, are deemed waived and excluded.

IMPORTANT : Please read these instructions
before operating your UVP Transilluminator
to familiarize yourself with its operation.

Introduction

The UVP High Performance Transilluminators offer
the researcher uniform and intense sources of ultra­violet light (radiation). The special design emits high
intensity excitation ultraviolet for back-illumination of
transparent fluorescent materials. Midrange ultravio­let (302nm) provides a highly sensitive method to de­tect double-stranded nucleic acids that are labeled
with fluorescent dyes such as ethidium bromide or
acridine orange. Single stranded nucleic acids may
be detected, but with a lower excitation wavelength
more sensitive for nucleic acid visualization than the 365nm model.

Note: Though UVP refers to the midrange UV waveband as 302nm, others refer to this region as 300nm

or 312nm. The spectral output of all these regions is the same.

UVP’s duty under the warranty is limited to replacement and/or repair of the defective part at the option of UVP, LLC. UVP shall
not be liable for any expenses or damages incurred by the purchaser except as expressly set forth herein, and in no event shall
UVP be liable for any special, incidental or consequential damages of any kind. This warranty does not supersede any
statutory rights that may be available in certain countries.

UVP … Providing Quality Products for the Researcher Since 1932. From its start in 1932, UVP has become a story of growth
and value fulfillment. Today, UVP services the needs of science, industry and education throughout the world. A special
significance of UVP research and development arises from our constant communication with customers. UVP continually
invites your comments toward the improvement of UVP products or development of custom types of ultraviolet light sources.
UVP will work with you through development and production of a product that meets your specific needs. From all of us at UVP,

Thank You.

Web Site: www.uvp.com

Corporate Headquarters: UVP, LLC 2066 W. 11th Street, Upland CA 91786

Tel: [phone] or [phone] Fax: [phone]

European Sales Operations: Ultra-Violet Products Ltd. Unit 1, Trinity Hall Farm Estate,

Nuffield Rd., Cambridge CB4 1TG UK Tel: [phone] Fax: [phone]

Important Safety Information

A word of caution: All UVP High Performance Transilluminators emit powerful sources of UV radiation that
will cause damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the
area are properly protected. Personnel should protect skin and eyes by wearing ultraviolet protection eyewear,
gloves and clothing when operating UV equipment.

It is recommended the transilluminator be installed and operated in a darkroom where access and expo­sure to UV is limited while the unit is in operation. If a darkroom is unavailable, UVP offers a wide selection
of darkrooms or shroud which provide protection from accidental exposure. For product information, con­tact UVP.

Each transilluminator is shipped with an ultraviolet blocking cover. Even though this cover blocks the UV
radiation emitted by the unit, UV Blocking Eyewear should be worn as well. UVP has a complete line of UV
Blocking Eyewear: Spectacles, Goggles and Faceshield designed for this purpose. For accessory part
numbers, refer to page number 4.

81-0161-01 Rev C

Description/Specifications

Part Wave- # of Intensity

Model Number length Volt/Hz/Amp Filter Size Tubes Style

TFM-20 95-0286-01 302nm 115V/60Hz/2.0 20 x 20 cm 4 x 25W High/Low
TFM-20 95-0286-02 302nm 230V/50Hz/2.0 20 x 20 cm 4 x 25W High/Low
TFM-20 95-0286-03 302nm 100V/50-60Hz/2.0 20 x 20 cm 4 x 25W High/Low
TFM-26 95-0285-01 302nm 115V/60Hz/2.0 21 x 26 cm 4 x 25W High/Low
TFM-26 95-0285-02 302nm 230V/50Hz/2.0 21 x 26 cm 4 x 25W High/Low
TFM-26 95-0285-03 302nm 100V/50-60Hz/2.0 21 x 26 cm 4 x 25W High/Low
TFM-30 95-0302-01 302nm 115V/60Hz/2.0 25 x 30 cm 4 x 25W High/Low
TFM-30 95-0302-02 302nm 230V/50Hz/2.0 25 x 30 cm 4 x 25W High/Low
TFM-30 95-0302-03 302nm 100V/50-60Hz/2.0 25 x 30 cm 4 x 25W High/Low
TFM-40 95-0283-04 302nm 115V/60Hz/2.0 20 x 40 cm 4 x 25W High/Low
TFM-40 95-0283-05 302nm 230V/50Hz/2.0 20 x 40 cm 4 x 25W High/Low
TFM-40 95-0283-06 302nm 100V/50-60Hz/2.0 20 x 40 cm 4 x 25W High/Low
TFL-40 95-0283-01 365nm 115V/60Hz/2.0 20 x 40 cm 4 x 25W High/Low
TFL-40 95-0283-02 365nm 230V/50Hz/2.0 20 x 40 cm 4 x 25W High/Low
TFL-40 95-0283-03 365nm 1005V/50-60Hz/2.0 20 x 40 cm 4 x 25W High/Low

8. To lower the UV intensity for extended viewing or sample work, simply press the Dual Intensity
Switch to low. A drop in the intensity will now occur. The fluorescence of the stained sample at this
time should diminish. As UV radiation is not visible to the eye, the UV transmitting filter does not
allow the passage of light up to 400nm (blue). A slightly dimming of this blue color is visible at this
time. A UV measuring device with the proper UV wavelength sensor can be used to provide actual
intensity reading (see Section 8 for Radiometer information).

9. After viewing/photographing the sample, turn the unit off.

10. Clean the surface with a damp soft cloth or sponge. Never use abrasive cleaners (can damage the
UV filter surface).

Maintenance, Repair and Technical Assistance

UVP offers technical support for all of its products. If you have any questions about the product’s use,
operation or repair, please call or fax UVP Customer Service in the US at [phone] or (909) 946­3197, Fax: [phone]; In Europe/UK: [phone]; Fax: [phone].

The dimensions of the High Performance UV Transilluminators are:
Width: 19.13” (486mm) Depth:13.25” (336mm) Height: 5.63” (143mm) Height in-

cludes cover

Operation

1. Place the transilluminator on a level work surface allowing air space around the bottom of the unit
and the work surface. This space allows for the proper air circulation through the unit.

2. Plug the female end of the power cord into the transilluminator. For 230V models, or those requir­ing special power cord connectors, ensure that the proper configuration of male connector or plug
has been properly connected to the power cord.

3. Plug the male end of the power cord into a properly grounded electrical outlet. The proper voltage
of the transilluminator is found on the product information label.

4. The transilluminator comes equipped with a UV Blocking Cover. If this cover is not in place or has
been removed, do not operate the unit without securing the cover. If the cover is missing, a UV
Blocking Faceshield must be worn to avoid UV exposure to the skin. UV Blocking Eyewear should
be worn even with the cover in place to avoid accidental UV exposure. The cover is reversible and
can open away from the researcher by removing the four screws (two on both ends of the transil­luminator) and rotating the transilluminator top 180o.

Note: A Returned Goods Authorization (RGA) number must be obtained from UVP Customer
Service before returning any product.

Changing the Replacement Tubes

1. Disconnect the transilluminator from the electrical supply.

5. Place gel/sample on the filter area. Gels may be placed on a UVP Gel-Tray (see Section 8 for part
numbers) which protects the filter surface from cuts and scratches. It is recommended that gloves
be worn to avoid contact with gel and staining agents.

6. Position the Dual Intensity Switch to the High operation position.

7. Press the ON/OFF switch to ON. The UV tubes within the unit should be glowing beneath the filter
after a momentary flickering during the start-up period.

2

2. A Phillips head screwdriver is required to remove the filter cover.

3. Carefully twist the UV tubes from their sockets.

4. Fit with the proper replacement tubes (refer to Section 9 for ordering information).

Cleaning the Transilluminator

The painted surfaces and filter areas of the transilluminator should be cleaned with water, soap, and a
sponge or cloth towel. Never use abrasive cleaners, solvent-based cleaners or scouring pads. Always
disconnect the transilluminator from the electrical power prior to cleaning.