UVP GelDoc-It TS3, ChemiDoc-It TS3 Installation And User Instructions Manual

TS3

GelDoc-It

®

& ChemiDoc-It

Installation and User Instructions

TS3

®

Imagers

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]
Web Site: www.uvp.com

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and ChemiDoc-It

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Table of Contents

Table of Contents .......................................................................................................................................... 2

1.0 Introduction ........................................................................................................................................... 4

2.0 Safety Instructions ................................................................................................................................ 5

2.1 General Notes .............................................................................................................................. 5

2.2 Safety Instructions – Operation and Maintenance ...................................................................... 5

3.0 System Components ............................................................................................................................ 6

3.1 Specifications ............................................................................................................................... 7

3.2 Built-In Touch Screen .................................................................................................................. 7

3.3 Cameras and Lenses ................................................................................................................... 8

3.4 Ethidium Bromide (EtBr) Emission Filter ..................................................................................... 8

3.5 Darkroom ..................................................................................................................................... 8

3.6 Fold Down Tray for Blots ............................................................................................................. 9

3.7 Transilluminator ........................................................................................................................... 9

3.8 LCD Touch Screen ...................................................................................................................... 9

3.9 VisionWorks touch ....................................................................................................................... 9

3.10 Fluorescent Focus Target ............................................................................................................ 9

3.11 Optional Equipment ................................................................................................................... 10

4.0 Setup Instructions and Installation ..................................................................................................... 12

4.1 Components .............................................................................................................................. 12

4.2 Connecting the Transilluminator and Accessories .................................................................... 12

4.3 Installing the Camera, Brackets and Cables ............................................................................. 13

5.0 Operating the System ......................................................................................................................... 16

5.1 Powering Up the System ........................................................................................................... 16

5.2 Preparing the Transilluminator for Use ...................................................................................... 16

6.0 Setting up VisionWorks touch ............................................................................................................ 17

6.2 Connecting to a Network ........................................................................................................... 17

6.2 Installing Drivers or Additional Software .................................................................................... 17

6.4 Setting Preferences ................................................................................................................... 17

7.0 Image Capture .................................................................................................................................... 20

7.1 Preparing for Image Capture ..................................................................................................... 20

7.2 Capturing an Image ................................................................................................................... 21

7.3 Image Capture Settings ............................................................................................................. 23

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7.4 Running Saved Templates ........................................................................................................ 28

8.0 Image Enhancement and Analysis ..................................................................................................... 30

8.1 Using the Gallery to Access Images .......................................................................................... 30

8.2 Compositing for Applications ..................................................................................................... 32

8.3 Additional Features .................................................................................................................... 35

9.0 Service Procedures ............................................................................................................................ 39

9.1 Return Procedure ...................................................................................................................... 39

9.2 Replacement Parts and Accessories ......................................................................................... 39

9.3 Troubleshooting ......................................................................................................................... 40

9.5 Technical Support ...................................................................................................................... 41

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and ChemiDoc-It

1.0 Introduction

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Imagers 4

The GelDoc-It
documenting fluorescent gel images.

In addition to gel documentation capabilities, the ChemiDoc-It
cooled, scientific-grade CCD camera allowing users to capture and document chemiluminescent
blot images, including Western blots.

The TS3 Imagers are self-contained, light-tight imaging systems with a built-in 15.6” touch screen
and computer and VisionWorks touch software for image acquisition and enhancement.

The ChemiDoc-It
placement, built-in overhead epi white lighting, a UV transilluminator and a five-position slide-out
emission filter wheel with an ethidium bromide (EtBr) emission filter included as standard. Save
images to the internal hard drive, to an external USB storage device or to a network drive for later
quantitative analysis or enhancement for publication.

UVP’s full package VisionWorks® Acquisition and Analysis software is included* with all TS3
Imagers for image analysis and quantitation of gels, blots and colony plates. VisionWorks
requires an external computer and cannot be operated directly on the TS3’s integrated computer.

*System/software configurations may vary by country. Contact UVP or authorized distributor for
details.

TS3

Imager is a high resolution imaging system capable of capturing and

TS3

Imager is equipped with a

TS3

darkroom, has a fold-down chemiluminescence tray with multiple levels for

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General warning

Caution: ultraviolet radiation

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and ChemiDoc-It

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Imagers 5

2.0 Safety Instructions

2.1 General Notes

For your own safety, please read this chapter carefully before operating. Observe all safety notes listed in
this user manual.

Symbols and signal words used

The user manual uses the following symbols and signal words to indicate safety hazards or warnings.

2.2 Safety Instructions – Operation and Maintenance

The system is designed for function, reliability, and safety. The unit may include shortwave UV, which is a
powerful source of UV radiation that will cause damage to unprotected eyes and skin.

For your own safety observe the following notes:

 The device must only be operated if all protective equipment is present, and the system is

properly installed and fully functional.

 Free access to the power plug and main power switch on the back of the unit must be ensured.
 Disconnect the power supply before servicing the system
 For UV protection and to extend the life of the UV transilluminator, the system incorporates a

customizable transilluminator shutoff timer built into the software. For additional information,
refer to Touch Screen Interface in this manual.

Caution: Do not override the UV safety switch located on the darkroom without the use of
protective equipment and face shields.

Caution: Shortwave UV (254 nm) is a powerful source of UV radiation that will cause
damage to unprotected eyes and skin. Before operating any unit, ensure all personnel in

the area are properly protected and that instructions for use of this equipment are followed.

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Camera and Lens
Located at Top of

Darkroom

15.6” Touch Screen

Computer Power

Button

USB Port

Built-In Overhead

White Lights

Five Position Slide­Out Emission Filter

Wheel

Transilluminator on

Roll-Out Tray

UV Safety Interlock

Switch & Override

BioLite™ Access Port

Power Indicator Lights

Fold down tray for

blots

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and ChemiDoc-It

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3.0 System Components

Refer to the packing slip and pictured components for specific parts and components included
with the system.

GelDoc-ItTS3 & ChemiDoc-ItTS3

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General Technical Data

Power Requirements

100/115V, 50/60Hz; 3.1 Amps at 120 Volts
230V, 50/60Hz; 1.55 Amps at 230 Volts
Mains supply voltage fluctuations are not to exceed 10

percent of the nominal supply voltage

Pollution Degree

2

Installation Category

II

Altitude

Up to 2000m

Ambient
Temperature

5°C to 40°C

Humidity

Maximum relative humidity of 80% for temperatures
up to 31°C, decreasing linearly to 50% maximum
relative humidity at 40°C

General Technical Data

Operating System

Windows® 8.1, 64-Bit

Connectivity Ports

1 USB (side of system)
6 connections for USB, VGA, Ethernet (rear of

system)

Wireless Networking
Capability

802.11 b/g/n

USB Flash Drive
Capacity

2 GB (minimum)

Internal Hard Drive

320GB (minimum)

Software

VisionWorks touch (touch screen)

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and ChemiDoc-It

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3.1 Specifications

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3.2 Built-In Touch Screen

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3.3 Cameras and Lenses

GelDoc-It

TS3

: The GelDoc-It

camera with a resolution of 5.0MP with USB 3.0 PC interface.

ChemiDoc-It

TS3

: The ChemiDoc-It
scientific-grade monochrome CCD camera. All cameras offer Peltier cooling and full 16-bit file bit
depth:

The BioChemi 515 camera has 2.1MP resolution (with zoom lens) and a 4.1MP (with
fixed lens) with Peltier cooling to -57°C from ambient. The BioChemi 515 has a peak
quantum efficiency of 50%.

The MegaCam 815 camera has 8.1 MP resolution with Peltier cooling to -57°C from
ambient. The MegaCam 815 has a peak quantum efficiency of 50%.

All camera settings are factory pre-set for optimum performance when viewing gels, films or
membranes under low light level conditions. Contact UVP Technical Support prior to making any
adjustments to internal camera settings.

TS3

is equipped with the GelCam 315 camera, a monochrome CMOS

TS3

is equipped with the BioChemi™ 515 or MegaCam 815

3.4 Ethidium Bromide (EtBr) Emission Filter

The ethidium bromide (50mm) UV-blocking bandpass interference filter blocks UV and IR
radiation emitted from the transilluminator. The filter is placed in the slide-out filter wheel
assembly on the side of the Imager. The filter allows visualization of fluorophores from 580­630nm, targeting the ethidium bromide emission peak of 605nm.

Additional filters are available for other specific fluorophores, including custom filters. Filters can
also be removed when imaging non-fluorescent media (including chemiluminescent blots, protein
gels, colony plates, etc.) in order to produce brighter images. Contact UVP for ordering
information.

3.5 Darkroom

The darkroom is light tight to provide optimal imaging conditions. Darkroom features include:

 Fold down tray for imaging closer to the lens (ChemiDoc-It TS3 only)
 Stylus for operating touch screen computer
 Epi (overhead) LED white light
 Roll-out transilluminator tray
 Five position motorized emission filter wheel
 Brackets and built-in power ports for installing optional epi UV modules
 Brackets for installing optional high-intensity halogen or xenon BioLite epi light guides
 Built-in power jumper cable for transilluminator
 Power cord for optional LED White Light Plate
 UV safety interlock switch with manual override to disable UV transillumination and

optional UV epi illumination when darkroom door is opened

Caution: Do not override the UV safety switch without the use of protective equipment
and face shields.

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Fluorescent Focus Target

VisionWorks touch software

Fold down tray

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and ChemiDoc-It

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3.6 Fold Down Tray for Blots

The ChemiDoc-It
tray located inside the darkroom. The tray folds down and
can slide up and down for multiple levels of placement.

TS3

Imager is equipped with a fold down

3.7 Transilluminator

The GelDoc-It
transilluminator models. UVP offers a variety of transilluminator configurations, including
Benchtop models with multiple wavelengths and variable intensities, as well as the highly uniform,
patented FirstLight transilluminator.

TS3

and ChemiDoc-It

Note: For UV protection and to extend the life of the UV transilluminator, the system
incorporates a customizable transilluminator shutoff timer built into the software. For
additional information, refer to the Lighting section of this manual.

3.8 LCD Touch Screen

The GelDoc-It
screen computer. The touch screen allows the user to perform a variety of tasks, including
previewing, capturing, saving and printing images, as well as selecting preference options,
without the use of an external mouse or keyboard. The screen can be pressed by finger or with
the included stylus.

TS3

and ChemiDoc-It

TS3

Imagers can accommodate UVP’s Benchtop and FirstLight®

TS3

Imagers contain a fully integrated 15.6-inch color touch

For users who prefer not to use the touch screen interface, an external keyboard and mouse are
included as standard. These components can be plugged into any available system USB ports.

3.9 VisionWorks touch

Image acquisition and hardware functions for the GelDoc-

TS3

It

and ChemiDoc-It

TS3

Imagers are controlled by using
the VisionWorks touch software interface. In addition to
image preview, capture and save functions, VisionWorks
touch controls camera and lens functions (exposure,
aperture, focus and zoom, where applicable), image
printing, lighting and filter selection, and other user
settings.

3.10 Fluorescent Focus Target

The UVP Fluorescent Focus Target fluoresces when
placed on a UV transilluminator or when exposed to
overhead UV. The Target provides sharp fluorescent
images to aid in adjusting the lens and camera settings for
ideal imaging results.

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Thermal Printer

Epi UV Module

LED White Light Plate

BioLite MultiSpectral Light Source

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3.11 Optional Equipment

UVP offers a variety of optional equipment to support the needs of varying laboratory
environments. Refer to Replacement Parts and Accessories at the end of this manual for
optional equipment part numbers.

BioLite MultiSpectral Light Source

The BioLite™ MultiSpectral Light Source utilizes high

intensity xenon or halogen lighting in conjunction with
fiber optic guides to a supply powerful, directed
illumination to a variety of fluorescent stained samples.
A wide range of excitation and emission filters enable
a wide spectral range of wavelengths, including red,
green, blue and NIR.

Thermal Printer

The thermal printer provides archive quality, 256
grayscale prints and five optional cost-effective print
sizes.

UV Modules for Overhead Lighting

A set of two 4-watt ultraviolet modules can be
connected inside the darkroom to provide epi UV
illumination. These modules can be switched on or off
in the Lighting menu within VisionWorks touch and
can also be removed from the darkroom and used as
standalone handheld lamps (as seen in the image to
the right).

LED White Light Plate

The LED White Light Plate emits high uniformity with
less than 5% coefficient of variance (CV). Plug the
LED White Light Plate directly into the power supply
within the darkroom and select “White Light Plate”
under the Lighting menu within VisionWorks touch.

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UV/White Converter Plate

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and ChemiDoc-It

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Converter Plates

An alternate to the LED White Light Plate, the
UV/White Converter Plate allows imaging of non­fluorescent stained media with an ultraviolet
transilluminator. The converter plate is specially coated
to convert 302nm UV to white light rather than using a
separate white light box.

The Visi-Blue™ Converter Plate (not shown)
converts UV to a safe 460-470nm wavelength
designed for use with blue excitation samples and
SYBR Green, SYPRO Orange and GFP stains.

VisionWorks® Analysis Software

All TS3 systems include* a copy of VisionWorks software for analysis of gels, plates and
membranes. The software features image enhancement, complete analysis tools and
reporting capabilities, and is ideal for users who require image analysis functions in addition
to the standard image acquisition capabilities of the VisionWorks touch.

NOTE: VisionWorks software must be installed and operated on an external computer.

*System/software configurations may vary by country. Contact UVP or authorized distributor for details.

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4.0 Setup Instructions and Installation

4.1 Components

When unpacking the GelDoc-It

 GelDoc-It

TS3

or ChemiDoc-It

 Camera, lens, mount, brackets and hardware
 Stylus
 Ethidium bromide (EtBr) emission filter
 Transilluminator
 Power cables
 USB flash drive (8 GB minimum)
 Keyboard and mouse
 VisionWorks Software on a flash drive (if included; varies by country)
 Supporting documentation
 Black chemi tray for blots (ChemiDoc-It TS3 only)
 Gel tray and gel ruler (GelDoc-It TS3 only)

When unpacking and setting up the darkroom, two people are required to move the darkroom.

TS3

and ChemiDoc-It

TS3

system

TS3

, the following items will be included:

Place the darkroom on a flat surface which can provide adequate support for up to 100 pounds.

WARNING: Do not attempt to perform any setup procedures while the system is
plugged in or powered on unless otherwise instructed.

Do not install the system in areas with high moisture, dust or high temperatures. Keep
the equipment away from motors or any other large magnetic equipment. This system is

designed for indoor use only.
Do not position the system so that it is difficult to access the power cable and operate

the main power switch at the back of the unit.

4.2 Connecting the Transilluminator and Accessories

1. Inside the darkroom, place the transilluminator on the roll-out tray. Connect the

transilluminator to the internal power jumper cable. Ensure that the green power switch on
the front of the transilluminator unit is in the ON position and that the desired wavelength or
intensity is selected.

Note: For UV protection and to extend the life of the UV transilluminator, the system
incorporates a customizable transilluminator shutoff timer built into the software. For
additional information, refer to the Lighting section of this manual.

2. If installing the LED White Light Plate, place the plate on top of the UV transilluminator and

connect the power cord coming from inside the darkroom to the back of the Plate.

3. If installing epi UV modules, place the modules in the brackets located at the top of the

darkroom. Plug the modules into the outlets provided inside the darkroom. Place the power
switches located on the modules in the ON position.

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4.3 Installing the Camera, Brackets and Cables

The camera will come equipped with a bracket, mount, brass thumb screws and black screws.

Note: Not all cameras, brackets and bases will look exactly as pictured below.

4.3.1 Installing the Bracket and Mount

1. Install the bracket and mount unit to the top of the darkroom using black screws and
brass thumbscrews.

4.3.2 Installing the Camera and Lens

After installing the bracket and mount unit, the camera and lens must be mounted to the
top of the darkroom.

1. If attached, pull the lens cover off the camera (do not unscrew).

2. Mount the camera making sure that the white label stating Front is facing the front of
the darkroom.

3. Secure the camera and lens to the bracket and mount unit, using brass
thumbscrews.

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