UVP GelDoc-Ite Imager User Manual

GelDoc-It

Imager

Installation and User Instructions

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UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

81-0334-01 Rev B

GelDoc-Ite Imager 2

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Introduction

The GelDoc-It® Imager enables easy, efficient and economical documentation and analysis of fluorescent
and non-fluorescent gels, membranes, film, plates and assays. Use UVP’s VisionWorks
Analysis software to capture images, to save images for later quantitative analysis, or for image
enhancement for publication purposes.

The GelDoc-It
FluorCam 220 camera with 1.3MP and optical zoom for ideal image capture. In addition, the Imager’s front
Viewer Window provides a look into the imaging darkroom, while the two side doors offer easy access to the
imaging surface for sample movement or excisi on.

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Imager contains a built-in UV transilluminator, standard ethidium bromide filter, and a

®

LS Acquis ition and

GelDoc-Ite Imager 3

White Light Power Switch

Darkroom Door Lock Knob

Filter Tray (located beneath

Bromide Filter

UV-Blocking Gel Viewer

Integrated UV

FluorCam 220 Camera and

Side Access Doors

UV Power Switch

UV Selector Knob

System Components

Refer to the packing slip and pictured components for specific parts and com pon ents included with the
system.

Zoom Lens

the camera) with Ethidium

Window

(on 2UV™ and 3UV™

models) or Intensity Selector

Knob (Single UV Models)

Transilluminator

Specifications

Power Requirements: 100/115V, 50/60Hz; 3.1 Amps at 120 Volts
230V, 50/60Hz; 1.55 Amps at 230 Volts
Mains supply voltage fluctuations are not to exceed 10 percent of the

nominal supply voltage

Pollution Degree: 2
Installation Category: II
Altitude: Up to 2000m
Ambient Temperature: 5°C to 40°C
Humidity: Maximum relative humidity of 80% for temperatures up to 31°C,

decreasing linearly to 50% maximum relative humidity at 40°C

Minimum Computer Requirements:
Processor: Pentium class, 1.6 GHz or higher
RAM: 1 GB (2GB Preferred)
Display: 1024 x 768
Operating System: Microsoft Windows XP Service Pack 2 or Newer (32-bit or 64-bit)
Internet Browser: Microsoft Internet Explorer 6.0 or later
Computer USB Ports: Computer must come equipped with a minimum of one USB port;

additional peripherals (mouse, keyboard, printer, etc.) may require
additional USB ports.

GelDoc-Ite Imager 4

Fluorescent Focus Target

Camera and Lens

The GelDoc-Ite Imager is equipped with a FluorCam 220 camera, a scientific-grade monochrome CCD
camera with a resolution of 1.3 megapixels (1280x1024). The FluorCam 220 utilizes a USB 2.0 PC
connection and is equipped with an 8-48mm optical zoom lens.

All camera settings are factory pre-set for optimum performance when viewing gels, films or
membranes under low light level conditions. Contact UVP Technical Support prior to making any
adjustments to the camera settings.

VisionWorksLS Acquisition and Analysis Software

The GelDoc-Ite is configured with VisionWorksLS Software for acquisition and analysis of gels, plates
and membranes. The software features image enhancement, complete analysis tools and reporting
capabilities, and is ideal for users who require robust image acquisition and analysis functions.

Ethidium Bromide (EtBr) Emission Filter

The ethidium bromide (50mm square) UV-blocking bandpass interference filter blocks UV and IR
radiation emitted from the transilluminator. The filter is placed in the Filter Tray located beneath the
camera/lens assembly. The filter allows visualization of fluorophores from 580-630nm, targeting the
ethidium bromide emission peak of 605nm.

Additional filters are available for other specific fluorophores, including custom filters. Filters can also be
removed when imaging non-fluorescent media (including protein gels, colony plates, etc.) in order to
produce brighter images. Contact UVP for filter ordering information.

Darkroom

The GelDoc-Ite Imager’s darkroom features includ e:

 Integrated UV transilluminator (available in 3UV, 2UV and single UV models)
 Epi (overhead) LED white light
 UV-Blocking Gel Viewer Window built into the darkroom door
 Two side doors for easy access to the imaging surface
 Single position emission filter tray
 UV safety interlock switch to disable UV transillumination when darkroom door is opened

Transilluminator

The GelDoc-Ite Imager is configured with UVP’s 3UV, 2UV or single UV Benchtop transilluminator
models. Refer to the packing slip for the specific transilluminator model included with your system.

Fluorescent Focus Target

The UVP Fluorescent Focus Target fluoresces when placed
on a UV transilluminator or when exposed to overhead UV.
The Target provides sharp fluorescent images to aid in
adjusting the lens and camera settings for ideal imag ing
results.