Olympus DP73 User Manual [ru]

INSTRUCTIONS

MICROSCOPE DIGITAL CAMERA

This instruction manual is for the Olympus Microscope Digital Camera Model DP73. To ensure the safety, obtain optimum performance and familiarize yourself fully with the use of this camera, we recommend that you study this manual thoroughly before operating the camera. For image operations including recording, editing and saving, please refer to the online manual for the cellSens / OLYMPUS Stream Software. Retain this instruction manual in an easily accessible place near the work desk for future reference.

DP73

AX8080

This device complies with the requirements of both directive 2004/108/EC concerning electromagnetic compatibility and directive 2006/95/EC concerning low voltage.

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste, but should be collected separately. Refer to your local Olympus distributor in EU for return and/or collection systems available in your country.

This equipment has been tested and found to comply with the limits for a Class A digital device,

NOTE:

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense.

FCC WARNING:

Changes or modifications not expressly approved by the party responsible for compliance

could void the user’s authority to operate the equipment.

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CONTENTS

— Be sure to read this chapter for safe use of the equipment. — 1-11

IMPORTANT

1 SYSTEM CHART ....................................................................................................................................................................................................15

2 NOMENCLATURE ...............................................................................................................................................................................................16

3 HARDWARE INSTALLATION ....................................................................................................................................................................19

Intended Use ...........................................................................................................................................................................................................................................................5

Conformity of the System .........................................................................................................................................................................................................................5

Handling Precautions ...................................................................................................................................................................................................................................13

Maintenance and Storage ......................................................................................................................................................................................................................14

2-1 DP73/DP73 (For Desktop Computer) ................................................................................................................................................................................................16

2-2 [OPTIONAL] DP-PXU PCIe Extension Unit (For Laptop Computer) ............................................................................................................18

Installing the Low-Profile Bracket ...................................................................................................................................................................................................19

Installing the PCIe Interface Board ..............................................................................................................................................................................................19

Installing the Camera Head ..................................................................................................................................................................................................................23

Connecting the Cables ..............................................................................................................................................................................................................................25

4 SOFTWARE INSTALLATION ......................................................................................................................................................................29

5 IMAGE ACQUISITION PROCEDURE ..........................................................................................................................................31

6 EXTERNAL TRIGGERING ..........................................................................................................................................................................33

7 SPECIFICATIONS ..................................................................................................................................................................................................35

9 TROUBLESHOOTING GUIDE ..............................................................................................................................................................40

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IMPORTANT

The DP73 microscope digital camera is designed to be connected to a camera adapter mounted on an Olympus UIS2/UIS series of optical microscope (not applicable to the LB series) for use in recording of microscopic magnified images at high speed and highest resolution while maintaining high picture quality and high color reproduction. The DP73 incorporates a variety of functions for supporting image recording under optimum conditions. When the DP73 microscope digital camera is used with a camera adapter or a microscope from a manufacturer other than Olympus, the optical performance may not be manifested fully.

If the equipment is used in a manner not specified by this manual, the safety of the user may be endangered. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

CAUTION

: Indicates a potentially hazardous situation which, if not avoided, may result in minor or

moderate injury or damage to the equipment or other property. It may also be used to alert against unsafe practices.

: Indicates commentary (for ease of operation and maintenance).

SAFETY PRECAUTIONS

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CAUTION

Never connect or disconnect the interface cable while the standby switch of the computer is set to ON. Otherwise, malfunction may result.

1. Before connecting or disconnecting the interface cable, make sure that the standby switch of the computer is set to OFF. When connecting the interface cable, push in the connector all the way and ensure that the connector will not slip out before setting the standby switch Do not move the computer or apply an impact to it while it is powered ON.

2. The cords and interface cables are vulnerable to bends or twists. Do not apply excessive force to them.

3. To prevent the microscope from toppling down, avoid using microscope attachments that may make the total height of the microscope above 1 meter when they are attached.

4. When installing the PCIe interface board, be sure to hold it by the edge. Never touch the board surface directly, as this will lead to malfunction.

to ON.

The area (IC) of the PCIe interface board becomes very hot during and immediately after use of the camera. Be careful never to touch them during and after use.

5. For handling of the computer, refer to the separately provided “Computer User’s Manual”.

6. Connect the power cord correctly and ensure that the grounding terminal of the power supply and wall outlet are properly connected. If the equipment is not grounded/earthed, Olympus can no longer warrant the electrical safety performance of the equipment.

7. Lay out the interface cable so that it does not contact the heat generating section such as the lamp housing of the microscope.

8. After operation or in case of abnormality, be sure to disconnect the power cord from the connector on the microscope or from the wall power outlet.

9. Never insert foreign objects into the air vents as this could result in fire and electrical shock.

Hot part

Computer and Software

The computer data may be destroyed by an unexpected event. Be sure to keep a backup of the data.

1. Olympus will not assume any liabilities for any damage incurred due to the use or non-usability of this system, including compensation for the lost data.

2. The computer used with this system should set up and run Microsoft Windows 7 Professional or Ultimate. For the OS in the computer, the user is requested to create a backup and retain it carefully. (Olympus does not support the matters related to the OS including its backup.) For details on the computer and Microsoft Windows 7, refer to their respective manuals.

3. Olympus guarantees the quality of this product in the factory shipment condition. Olympus will not assume any liabilities for the operation errors and functional faults incurred due to the alteration of the environmental setup (including BIOS change), installation of other software or addition of hardware to the computer by the user.

4. When the HDD free space reduces, the data processing speed may slow extremely or errors may occur frequently. To prevent this, delete unnecessary data files frequently. For how to delete data files, refer to the manuals for Microsoft Windows 7.

5. Never attempt to delete or rename the folders and files installed by the provided installer software. Otherwise, the software may get unable to be started up.

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6. Do not open the housing of the computer and touch the power supply or the circuit board’s heat generating section right after use as it may burn your hand. Wait until the internal temperature drops sufficiently.

7. Sharp edges inside the computer may cut your fingers, so take extra care.

8. Use a computer that complies with the safety standards of your country.

Intended Use

This device is intended to be used for the capture of digital images for non-clinical diagnostic purposes.

Conformity of the System

Restrictions in Use

1. The applicable camera adapter is the U-TV0.5XC-3, U-TV0.63XC-1-2, MVX-TV0.63XC, U-TV1XC or the combination of U-TV1X-2 and U-CMAD3. The U-TV0.5XC should not be used because it deteriorates the image flatness. A camera adapter with a magnification below 0.5X cannot be used because part of image will be obscured.

2. When the DP73 is connected to the rear port of the U-DPT(double port tube) or U-MPH(multi port head), the peripheral part of the recorded image may be deteriorated due to the optical performance of the U-DPT or U-MPH.

3. When the U-TV0.5/C-2/U-TV0.5/C-3/U-TV0.63XC-1-2(C mount camera adapter) is used, using two or more intermediate attachments* may obscure the peripheral part of the field of view or may make flare noticeable.

* Example of two intermediate attachments with BX microscope :

Reflected light illuminator + intermediate attachment equivalent to the U-CA.

4. Under fluorescent ring illumination or other AC-driven illumination such as a phase control light intensity adjusting illumination system, the following phenomena may be observed by illumination light flickering because of high light intensity and shortened exposure time :

· Flickering of the displayed image.

· Instability in exposure.

· Hatching patterns in pixel shift recording (4800 x 3600 or 2400 x 1800 pixels, 3CCD mode). However, provided that the brightness can be adjusted using the light intensity control knob or ND filters, the above phenomena may be attenuated by adjusting the brightness so that the exposure time exceeds 1/50 sec. For details on the microscope models using AC-driven illumination, contact Olympus.

5. Combinations of this product and non-Olympus microscopes have not been evaluated extensively. Non-Olympus microscopes and commercially available C-mount lenses can be used provided that they match a CCD with a size of no less than 1/1.8 inch and the lens projection length from the C-mount body attaching section is no more than 4.5 mm. However, problems due to optical adaptability, such as shading, may be observed.

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6. When the specimen has a low contrast (near transparent) or high reflectance (mirror status) and the aperture iris diaphragm is stopped down near the smallest aperture, spot flare may be noticeable.

7. When the edge of a non-transmitting object is observed under the STM6 (small measuring microscope) transmitted illumination, flare may be noticeable due to the difference in brightness between the transmitted sections (over-exposure) and non-transmitting sections (under-exposure). To reduce the flare, set a lower exposure using the exposure correction function or setting the exposure manually.

8. When a low-power objective (below 4X) is used, the peripheral part of the field of view may be obscured. In this case, use an ultralow-magnification condenser (U-ULC-2).

9. When the U-CFU (real time confocal unit) is used, it is required to set the exposure to a longer period than 1/30 sec. using the manual exposure mode and control the brightness by engaging or disengaging ND filters.

10. When the specimen has large difference in brightness, particularly when the bright part of the specimen comes on the upper part of the image, red and horizontal flare may be observed. It is noticeable when the AS is being closed. The flare will get unnoticeable when the AS is being opened, though the flare may not be completely removed.

11. When a specimen with high reflectivity is observed with reflected light brightfield observation through the beam splitter of the eyepiece/camera light path of a trinocular tube in combination with the U-TV0.5XC camera adapter, the image in the area outside the CCD’s effective image pickup area may be observed as vague ghosts in the peripheral area of the visual field of eyepiece.

12. Flare may be produced during reflected light darkfield observation under overexposure To reduce the flare, use the exposure correction function or reduce the exposure with manual exposure control.

13. During image acquisition with pixel shifting (4800 x 3600 or 2400 x 1800 pixels, 3CCD mode), the image may be disturbed if the specimen is moved.

14. If the camera or microscope is vibrated during image acquisition with pixel shifting (44800 x 3600 or 2400 x 1800 pixels, 3CCD mode), the image will be disturbed. There are several causes for the vibration which are for example the operation of keyboard/mouse on the same table where the microscope equipped with the camera is placed, or vibration from the instrument erquipped with an air cooling fan, etc.

Operating Environment

Temperature: 10 to 35°C. Humidity: 20% to 85% (without condensation). See page 39 for details.

Recommended Monitor Specifications

· A monitor with the 1280 x 1024 or larger full-color display capability.

· An Adobe RGB compatible monitor, provided that the camera head is used in the Adobe RGB mode.

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Recommended Computer Specifications – Desktop Computer –

1. Computer requirements

PC/AT compatible

CPU Intel Core series 1.8 GHz or greater [Core2 Duo E6400, 2.13 GHz or greater recommended] RAM 4 GB or more HDD Free space 1 GB or more Graphic PCI Express X16 VGA card with 1280 x 1024 or more and 32-bit color capability.

On-board graphic also acceptable.

Extension slot PCI Express Rev. 1.0a or later.

Half-size or low-profile PCIe board compatible (106.7 mm x 174.6 mm)

OS Windows 7 Professional/Ultimate (64 bit)

Language: English or Japanese

Power 250 W or more. (With CE marking)

* There needs to be the FDD power cable, HDD(4-pin) power cable or SATA power cable

which is not occupied.

2. CPU We do not guarantee performance if the computer uses a CPU other than or incompatible with Core series or uses a non-Intel chipset.

3. HDD free space The HDD free space (the value 1GB or more in Page 9) is the space that does not cause a special problem when the system is installed or run. The space required for saving an image file in the HDD is slightly more than 5.6 MB with a 1600 x 1200-pixel (24-bit) non-compressed image and slightly more than 50 MB with a 4800 x 3600-pixel (24-bit) non-compressed image. In consequence, the HDD should also provide a considerably large space for saving these image files. When saving movies in the HDD stack, the space required for saving a movie is about 80 MB (max.) per second. The movie recording time is limited according to the HDD free space.

4. RAM If a RAM other than a PC2700 or greater, dual-channel RAM is used, the full-size live frame rate may drop.

5. Monitor Us an Adobe RGB compatible monitor when using the camera head in the Adobe RGB mode. Optimum color reproduction is not available if the sRGB/Adobe RGB setting of the camera head does not match the setting of the monitor.

6. Sequential connection of PCIe units Up to two PCIe units including the DP73 and a PCI interface board of the DP72/DP71/DP70/DP30BW or FV1000 (FV10-ASW-V1.5 or later) can be connected in series. Series connection of the FV300/FV500 is not possible. However, their simultaneous operation is not available so it is required to select either PCI interface operation.

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7. Power supply The PCIe interface board should be powered by connecting the FDD power supply connector from the ATX power supply in the computer. If your computer does not have an available FDD power cable or the FDD power cable is too short, use the provided HDD(4-pin) to FDD power conversion cable for the power supply. And if your computer does not have an available HDD power cable (4-pin), use the provided SATA-to-HDD(4pin) power conversion adapter and HDD(4-pin)-to-FDD power conversion cable for the power supply.

Recommended Computer Specifications – Laptop Computer –

1. Computer requirements

PC/AT compatible

CPU Intel Core series 1.8 GHz or greater [Core2 Duo T7300, 2.0 GHz or greater recommended] RAM 4 GB or more HDD Free space 1GB or more Graphic On-board graphic with 1280 x 1024 or more and 32-bit color capability. Card slot ExpressCard/34 or ExpressCard/54 OS Windows 7 Professional/Ultimate (64 bit)

Language: English or Japanese

2. CPU We do not guarantee performance if the computer uses a CPU other than or incompatible with Core series or uses a non-Intel chipset.

4. RAM If a RAM other than a PC2700 or greater, dual-channel RAM is used, the full-size live frame rate may drop.

6. ExpressCard The ExpressCard tends to slip out easily from the computer. Be sure not to disconnect the ExpressCard during operation. For protection from possible damage, do not apply exessive force to the ExpressCard. Insert or remove the ExpressCard after the PC is shut down.

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Handling Precautions

1. This camera head is a precision instrument. Handle it with care and avoid subjecting it to a sudden or severe impact.

2. The image displayed on the monitor may be affected when it is used at a place close to equipment generating strong electromagnetic waves. This is not a malfunction and will not affect the actual image being recorded. To avoid interference during operation, keep the system far from any source of electromagnetic waves.

3. As the camera head is heavy, hold it carefully not to drop when mounting or storing.

4. Do not use the camera in areas where it may be subjected to direct sunlight, high temperature, humidity, dust, or vibrations. (For the operating environment conditions, see chapter 8, “SPECIFICATIONS“ on page 35.)

5. Be sure to install the PCIe Extension Unit without blocking the opening for ventilation provided on it.

6. The camera head needs to be calibrated periodically (approx. every 3 months) for the level variations caused by the influence of cosmic rays. For the calibration method, refer to the CCD Calibration in the online manual for the cellSens or OLYMPUS Stream software.

7. As a countermeasure against computer virus infection, it is recommended to install an anti-virus software on the PC. Operation speeds of the cellSens/OLYMPUS Stream may slow down depending on the anti-virus software.

Maintenance and Storage

1. To clean the lenses and other glass components, simply blow away dirt using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it with gauze slightly moistened with commercially available absolute alcohol.

CAUTION

Since the absolute alcohol is highly flammable, it must be handled carefully. To prevent fire ignition, be sure to keep it away from open flames or potential sources of electric sparks – for example, electrical equipment that is being switched on or off. Also remember to always use these chemicals only in a well-ventilated room.

2. Parts other than the glass components should be cleaned by wiping with a clean cloth. Do not use organic solvents to remove major stains. Use a soft cloth slightly moistened with a neutral detergent solution.

3. Do not disassemble any part of the camera as this could result in malfunction or reduced performance.

4. When the camera is removed from microscope for storage, be sure to put the C-mount cap included in the camera, which is necessary to protect the IR cut filter provided inside.

5. When the camera is stored, be sure to keep the C-mount portion downward, since it tends to roll over.

6. When disposing of this product, check your local regulations and rules and be sure to observe them strictly.

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1 SYSTEM CHART

DP73 / DP73WDR

External trigger cable

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Camera head

Camera interface cable

C-mount camera adapter

DP-PXU

PCIe extension box

Consult Olympus for the microscope, camera adapter and DP2-TWAIN to be used together.

PCIe interface board

Low-profile bracket

PCIe x1 cable

AC adapter

SATA-to-HDD power conversion adapter

HDD-to-FDD power conversion cable ( Not used when the DP-PXU is combined. )

ExpressCard

Desktop PC

Laptop PC

Resolution 1260 x 1024 or higher

cellSens Software

Monitor

2 NOMENCLATURE

2-1 DP73 / DP73WDR (For Desktop Computer)

Camera Head

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CAUTION

Any equipment connected to the camera head should be an Olympus-designated product or a product in compliance with the requirements of IEC60950 or CISPR22/24. If equipment other than these products is connected, Olympus cannot guarantee any performance of the camera.

Connector (P.25)

C-mount thread (P.23)

PCIe Interface Board

Trigger cable connector (P.28)

Interface cable connector (P.25)

(PCI standard half-size, low-profile size compatible)

External trigger cable (P.28)

Software

Software (optional) cellSens / OLYMPUS Stream

(DVD-ROM)

Dongle

Low-profile bracket (P.19)

SATA-to-HDD(4-pin) power conversion adapter

Clamping screws

Interface cable (P.25)

SATA-to-FDD power conversion cable (P.19)

2-2 [OPTIONAL] DP-PXU PCIe Extension Unit (For Laptop Computer)

PCIe Extension Box

PCIe cable connector (P.26)

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AC adapter connector (P.27)

ExpressCard

PCIe cable connector (P.26)

PCIe x1 cable (P.26)

Vertical installation plates (P.22)

With 2 screws

AC Adapter

Power cord connector (P.27)

3 HARDWARE INSTALLATION

Installing the Low-Profile Bracket

If the computer’s extension slot is of the low-profile specifications or

Fig. 1

CAUTION

the PCIe extension box is used, it is required to replace the existing bracket with the low-profile bracket.

Prepare flat-blade and Phillips screwdrivers and remove the “ – ” screws

Replace the existing bracket with the low-profile bracket attach screws

Do not provide excessive force when tightening the screws of PCIE I/F boad, since they may be damaged if tightened excessively.

Installing the PCIe Interface Board

DP73 / DP73WDR (For Desktop Computer) Set (Figs. 2 & 3)

and the “ + ” screw b.

a b

(Fig. 2 - Fig. 6)

· Before installing the PCIe interface board in the computer, be sure to read the instruction manual for the computer in order not to damage it.

· Be sure to turn off the computer and peripherals and unplug their power cords before installing the PCIe interface board.

· To avoid damage due to static electricity, touch an unpainted metallic surface of the computer with your hand to discharge the static electricity before installation.

· The SATA-to-HDD(4-pin) power conversion adapter is sensi- tive to excessive force. Please handle it with care.

(Fig. 1)

and

Open the housing of the computer.

1 2

Remove the clamping screw PCIe slot on the motherboard and remove the cover.

of the slot cover a of an unused

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Insert the PCIe interface board

Fig. 2

3 4

Fig. 3

board surface directly by hand, and attach the slot cover using the

clamping screw

Connect the FDD power cable supply to the power connector If your computer does not have an available FDD power cable or the FDD power cable is too short, connect the provided HDD-to-FDD power conversion cable from the computer’s HDD power cable to the power connector And if your computer does not have an available HDD(4-pin) power cable, connect the provided SATA-to-HDD(4-pin) power conversion adapter and HDD(4-pin)-to-FDD power conversion cable from the computer’s SATA power cable to the power connector PCIe interface board

Close the computer housing.

removed above.

carefully without touching the

from the computer’s power

on the PCIe interface board c.

on the

Optional DP-PXU (Figs. 4 & 5)

Using a Phillips screwdriver, remove the six screws a of the top cover of the extension box and then remove the cover. .

Remove the bracket clamping screw

Connect the PCIe interface board

connector

Connect the connector

Attach the clamping screw

to the PCIe interface board.

removed above.

with low-profile bracket to the

Fig. 4

2 5

A thermal conduction sheet cover. Remove the tapes without displacing the thermal conductive sheet (Fig. 5).

Place the top cover in the original position and attach the clamping

screws

is attached on the back of the top

and the protective sheet h carefully

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Fig. 5

Installing the PCIe Extension Box (Fig. 6)

The PCIe extension box can be installed either horizontally or vertically.

Fig. 6

Horizontal Installation

Place the PCIe extension box on the desktop so that the surface with rubber feet faces down.

Vertical Installation

Attach the provided plates a to the side panel of the extension box using provided screws

Installing the Camera Head

(Fig. 7)

Fig. 7

CAUTION

· The C-mount thread has a sharp edge that should not be touched.

· The camera head and camera adapter are precision modules. Be careful not to drop them during attaching or detaching.

The following procedure deals with the case using the U-TV1XC C-mount camera adapter.

Screw in the U-TV1XC C-mount camera adapter

thread

at the bottom of the camera head.

into the mount

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Field number 22

Fig. 8

0.63X (FN 14)

0.5X (FN 17.6)

1X (FN 8.8)

CAUTION

· If the acquired field is as shown in Fig. 8, use a camera adapter having magnification of 0.5X to 1X. (If a 0.35X camera adapter is used, the peripheral part of the image will be obscured.)

· If a C-mount camera adapter from other manufacturer than Olympus is used, the optical performance of the system may not be manifested fully.

Be careful in using other manufacturer’s C-mount camera

adapter or C-mount lens having a thread length

4.5 mm. Otherwise, the threaded section will hit the inside of the camera head and cause damage to it.

Be sure to adjust the parfocality between the C-mount camera adapter and the eyepieces. Otherwise, the image through the camera and the image through the eyepieces are not parfocal. For the parfocality adjustment method, refer to the instruction manual for the camera adapter in use.

over

Connecting the Cables

(Fig. 9 - Fig. 13)

Computer

PCIe Extension Box

Fig. 9

Fig. 10

CAUTION

· The cords and cables are vulnerable to bend or twist. Do not apply excessive force to them.

· Be sure to switch off the computer before proceeding to the connections.

· Always use the cables designated by Olympus.

· When connecting the cable, insert the connector plug in the proper direction.

· Keep the cables well away from the equipments generating high heats, such as the lamp housing of microscope.

Cable side Camera head/

Computer/

PCIe Extension Box side

Connecting the Interface Cable (Figs. 9 & 10)

Insert the connector of the interface cable all the way into the connector on both sides of the connector, and check that the interface cable will not slip out.

Insert the connector of the connector the clamping screws on both sides of the connector, and check that

the interface cable will not slip out.

of the camera head and secure the clamping screws

interface cable all the way into the

of the Computer or PCIe Extension Box and secure

Connecting the ExpressCard and PCIe x1 Cable (Fig. 11)

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To ExpressCard

Fig. 11

CAUTION

Insert the cable connectors all the way.

Insert the ExpressCard all the way into the card insertion slot* of the

laptop computer.

* ExpressCard/34 slot: Hold the card with the surface marked

“INSERT” facing up and insert. ExpressCard/54 slot: Hold the card with the surface marked “INSERT” facing up and insert it along the left edge of the slot.

Check the orientation of the connector of the PCIe x1 cable and insert it into the connector on the PCIe extension box.

Insert the connector into the ExpressCard.

Connecting the AC Cable (Fig. 12)

CAUTION

Fig. 12

· Insert the cable and power cord connectors all the way.

· Always use the power cord provided by Olympus. If no power cord is provided with the camera head, please select the proper power cord by referring to chapter “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual.

· If the equipment is not grounded/earthed, Olympus can no longer warrant the electrical safety performance of the equipment.

Insert the connector of the AC adapter into the connector on the PCIe extension box.

Insert the connector of the power cord to the connector on the AC adapter.

Insert the power cord plug into the power outlet.

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Computer

2 3

b c

PCIe Extension Box

Fig. 13

Connecting the External Trigger Cable (Fig. 13)

Insert the connector of the external trigger cable to the connector

on the PCIe interface board in the computer or the PCIe

extension box.

When using the trigger input, connect the red cable (marked “I” )

to the BNC connector.

When using the trigger output, connect the white cable (marked

CAUTION

“O” )

The external triggering is available only when the cellSens / OLYMPUS Stream software is run.

to the BNC connector.

4 SOFTWARE INSTALLATION

Software cellSens / OLYMPUS Stream

Install the software cellSens / OLYMPUS Stream by referring to the cellSens / OLYMPUS Stream instruction manual.

Image acquisition software DP2-TWAIN

When you want to use the Image acquisition software DP2-TWAIN, consult Olympus.

Before Installation

· Quit all running applications before installing cellSens or OLYMPUS Stream.

· The software cannot be installed unless the user account is registered as “computer administrator.” If the user account is registered as a “Restricted account,” change it to the “Computer administrator” account. (For the user account registration, refer to the instruction manuals for your computer.)

Trademark Information

Windows is a registered trademark of Microsoft Corporation. All other brand and product names are trademarks or registered trademarks of their respective owners.

(Applicable OS: Windows® 7)

Selection of the devices for DP-PC-S/DP-PC and DP-PCWDR

Refer to the following windows at the selection of devices in the cellSens / OLYMPUS Stream. An device list is displayed at the time of first start up of the software. The device list can be also displayed by clicking the tabs of [Acquire], [Devices] and then [Device list] on the Menu bar.

DP73

In case DP73 is used

In case DP73WDR is used

5 IMAGE ACQUISITION PROCEDURE

For the details, refer to the Instruction Manual or Help of Software you will use.

Brightfield Observation

Set up the microscope.

Set the exposure mode.

Exposure mode?

Auto

Select the area (30%, 1%,

0.1%, full-image).

Adjust the white balance.

Perform framing and focusing.

Set the exposure time.

Manual

Reconfirmation / fine adjustment as required

Exposure mode?

Auto

Move the area and correct exposure.

Adjust the white balance.

Record an image.

Save the image.

Fine-adjust the exposure time.

Manual

Fluorescence Observation

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Set up the microscope.

Set the exposure mode.

Exposure mode?

SFL Auto

Select the area (30%, 1%,

0.1%, full-image).

Adjust the black balance.

Perform framing and focusing.

Set the exposure time.

Manual

Reconfirmation / fine adjustment as required

Exposure mode?

SFL Auto

Move the area and correct exposure.

Adjust the black balance.

Record an image.

Save the image.

Fine-adjust the exposure time.

Manual

6 EXTERNAL TRIGGERING

When the cellSens / OLYMPUS Stream software is used, the DP73 can record still images or control a commercially available shutter based on an external trigger signal.

Trigger Input

· The trigger input from external equipment can be used to start still image acquisition using the cellSens / OLYMPUS Stream.

· The trigger input is detected at the edge and the cellSens / OLYMPUS Stream starts to acquire the images based on the preset exposure time. The positive and negative logics can be switched with the cellSens / OLYMPUS Stream.

· The exposure is started within 100 μsec after the trigger input.

· The trigger input is a TTL compatible signal.

: 2.0 V (min.). VIL: 0.8 V (max.).

100μsec. or less

Trigger input

Exposure

Readout & transfer to PC

Trigger Input Timing Chart (Positive logic)

Trigger Output

· The trigger output can be used to control a commercially available shutter.

· The positive and negative logics of the trigger output can be switched with the cellSens / OLYMPUS Stream.

· The trigger output is interlocked with the shutter release/close operations of the cellSens / OLYMPUS Stream.

· The external trigger signal is output by starting still image acquisition when the shutter is closed.

· The time lag from the trigger output to the start of exposure can be set between 0 and 2 sec. on the cellSens / OLYMPUS Stream.

· The trigger output is a TTL compatible signal.

: 2.4 V (min.). VOL: 0.4 V (max.).

Exposure

time lag

Trigger output

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Exposure

Readout & transfer to PC

Trigger Output Timing Chart in Still Image Recording (Positive logic)

7 SPECIFICATIONS

DP73 Specifications

* These items can be set from the cellSens / OLYMPUS Stream. ** The values of these items are variable depending on the exposure time setting and the computer operating situations.

Item Specifications

Camera system Single-CCD color camera

Pixel shifting type

Image pickup device 1/1.8-inch color CCD

Total pixels: 2.1 million pixels Effective pixels: 2.01 million pixels Pixel pitch: 4.40 μm (H) x 4.40 μm (V) Scanning method: progressive scanning

Cooling Peltier cooling

Natural air cooling CCD temperature: room temperature - 10°C (max.)

Recorded image sizes 4800 x 3600 (pixel shifting, 3CCD mode*)

2400 x 1800 (pixel shifting, 3CCD mode*) 1600 x 1200 (1 x 1, 3CCD mode)* 800 x 600 (1 x 1) 800 x 600 (2 x 2) * 3CCD mode is an application of the pixel shift function, which is the mode

to acquire the image with RGB color in every pixel.

Camera mount C-mount

Item Specifications

ISO speed ISO 100/200/400/800/1600 equivalent A/D 14 bits Exposure control Exposure modes Auto

SFL Auto

Manual AE lock Available Exposure correc-

tion Metering area Full image

Exposure time 1/44,000 to 60 sec Binning 2 x 2 Live frame rate** 1600 x 1200 (1 x 1): 15 fps

Color modes Color

Correction range: ±2.0 EV. Step: 1/3 EV

30%

0.1% The metering area is freely movable.

800 x 600 (1 x 1): 15 fps

800 x 600 (2 x 2): 27 fps

standard gray scale

custom gray scale

DP73

Item Specifications

External triggering* Input:

Random trigger input (Edge trigger mode)

Output:

Trigger output With exposure time lag (0 to 2 sec. in 10 msec. steps) TTL compatible

Image accumulation* Modes Integral, averaging

Accumulation count 64 frames (max.)

White balance modes Area-specified auto

Manual

Black balance modes Area-specified auto

Manual

Contrast modes Low

Standard

High

Linear

Sharpness filter Low

Standard

High

Wide dynamic range correction Available (only with the DP-PCWDR) Pixel shift bleaching correction Available Color space* sRGB

Adobe RGB

Item Specifications

Focus indicator Contrast bar

Line profile

Interval recording* Interval: 1 sec. to 24 h.59 m.59 sec

Number of shootable frames: 3000

Image file formats Dependent on the application software Preview quality modes Standard / Medium / High Computer interface PCI Express Rev. 1.0a or later

Compatible with half-size or low-profile size

ExpressCard/34 (also usable for ExpressCard/54)

Compatible OS RAM: 4 GB or more

OS: Windows 7 Professional/Ultimate (64 bit)

Language: English or Japanese

DP73

Item Specifications

Dimensions & weight <DP73>

Camera head

PCIe interface board 181(W) x 121(D) x 21.6(H) mm (excluding projections), approx. 200 grams Interface cable Approx. 2.8 meters External trigger cable Approx. 0.2 meter < PCIe Extension Unit> PCIe extension box 203(W) x 165(D) x 28(H) mm (excluding projections), approx. 720 grams ExpressCard 100(W) x 34(D) x 11(H) mm (excluding projections), approx. 25 grams AC adapter 61(W) x 133(D) x 41(H) mm (excluding projections), approx. 480 grams

PCIe x1 cable Approx. 1.7 meter

Storage environment -20 to 60 °C

Operating environment · Indoor use.

185.4(

) x 77.6(H) mm (excluding projections), approx. 900 grams

Cord length: Approx. 2.0 meter

100-240V 2A 50/60Hz

10 % to 90 % (without condensation)

· Altitude: max. 2000 meters

· Ambient temperature: 10° to 35°C (50° to 95° F)

· Relative humidity: 20% to 85% (without condensation)

· Supply voltage fluctuations; ±10%.

· Pollution degree: 2 (in accordance with IEC60664-1)

· Installation/Overvoltage category: II (in accordance with IEC60664-1)

9 TROUBLESHOOTING GUIDE

Under certain conditions, the performance of the camera may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact Olympus for assistance.

Problem Cause Remedy Page

The computer will not start up.

Live image is not displayed.

The camera and computer are connected improperly.

The software is installed improperly. Install the software. 29 The camera and computer are

connected improperly. The microscope illumination is off.

The microscope is not set to the camera light path. The illumination or specimen focusing is adjusted improperly.

The ISO speed or exposure time is set improperly.

Connect the camera head and computer properly.

Turn on the microscope illumination, adjust the lighting and focusing correctly, and select the camera light path.

Set the ISO speed, exposure mode, exposure time and level properly.

DP73

–

(Online

manual)

Problem Cause Remedy Page

Still images cannot be recorded.

The camera and computer are connected improperly.

The cellSens or OLYMPUS Stream is processing recording.

The cellSens / OLYMPUS Stream is processing file save, etc.

The computer memory is insufficient.

Connect the camera head and computer properly.

Wait until the recording processing completes before starting recording of the next image. In certain cases, it may be required to press the Cancel button on the status bar and record the image again.

Wait until the processing completes before starting recording of the next image.

Exit from other software before retrying recording.

(Online

manual)

(Online

manual)

–

Problem Cause Remedy Page

Picture is too bright.

Exposure correction is set in the + direction.

The metering area is set to a dark area outside the region of interest.

AE lock, which was set when the exposure time was longer than the currently required exposure time, is active.

The input highlight level adjustment is too low.

The microscope illumination is too bright.

Return the exposure correction value to 0 and set the desired exposure correction value.

Move the metering area to the area where you want to obtain optimum exposure.

Cancel AE lock.

Reset the current level adjustment and adjust the optimum level again.

Reduce the microscope illumination intensity or engage an ND filter to reduce brightness.

DP73

(Online

manual)

(Online

manual)

(Online

manual)

(Online

manual)

–

Problem Cause Remedy Page

Picture is too dark.

Exposure correction is set in the – direction.

The metering area is set to a bright area outside the region of interest.

AE lock, which was set when the exposure time was shorter than the currently required exposure time, is active.

The output highlight level adjustment is too low.

The microscope illumination is too dark.

Return the exposure correction value to 0 and set the desired exposure correction value.

Move the metering area to the area where you want to obtain optimum exposure.

Cancel AE lock.

Reset the current level adjustment and adjust the optimum level again.

Increase the microscope illumination intensity or disengage the existing ND filter to increase brightness.

(Online

manual)

(Online

manual)

(Online

manual)

(Online

manual)

–

Problem Cause Remedy Page

The colors in the picture are strange.

The area selected in white balance adjustment was improper.

The RGB balance is adjusted improperly in manual white balance adjustment.

The area selected in black balance adjustment was improper.

The screen color setting of the computer is incorrect.

Color spaces (sRBG/AdobeRGB) of the camera and the monitor are different.

Select a white area with the rectangular white balance adjustment area.

Perform manual white balance adjustment to adjust the RGB color balance to obtain optimum colors.

Select a black area with the rectangular black balance adjustment area.

Set the computer display color to 24-bit color or higher. The recommended setting is 32-bit color.

Set the Color space (sRGB/ Adbe RGB) of the camera so that it becomes identical to that of the monitor.

DP73

(Online

manual)

(Online

manual)

(Online

manual)

–

(Online

manual)

Problem Cause Remedy Page

The picture is not in focus. The specimen is not in focus

properly. The aperture iris diaphragm of the

condenser is open too wide. The field iris diaphragm is not set

properly.

Lens components of the microscope are contaminated or the cover glass on the front of the camera is stained.

The microscope and/or camera are subjected to vibration during recording.

The 4140 x 3096 and 2070 x 1548 images are not neat.

The camera is subjected to vibration during recording.

Adjust the focus correctly with the fine adjustment knob.

Close the aperture iris diaphragm a little.

Adjust the field iris diaphragm until the image circumscribes the field of view.

Clean the objective, camera adapter lens, condenser and/or window lens of the microscope, or clean the cover glass on the bottom of the camera head.

Acquire images in an environment in which the microscope and camera are not vibrated. It is effective to use an anti-vibration bench.

–

Problem Cause Remedy Page

It is impossible to open the file correctly, by the software other than cellSens/OLYMPUS Stream, in which the image acquired by 12bit color/12bit grey scale is saved. Or, the image in black color is displayed (including the icon on the window).

The cellSens or OLYMPUS Stream window is not displayed correctly or the menu characters are not displayed correctly.

External trigger signal is not output. External triggering is not enabled. Enable external triggering on the

Still image cannot be acquired using the external trigger input.

Effective data is low 12 bits out of 16 bits file format. However, your software does not accept 16 bits format or use high 8 bits for display.

The resolution setting of the screen is incorrect.

The large font has been selected for the font size of the screen.

External triggering is not enabled. Enable external triggering on the

Use cellSens/OLYMPUS Stream to open the file.

Set the resolution setting at 1280 x 1024 or more in the property of the screen.

Select a small font in the property of the screen.

cellSens / OLYMPUS Stream.

DP73

–

(Online

manual)

(Online

manual)

PROPER SELECTION OF THE POWER SUPPLY CORD

If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “Specifications” and “Certified Cord” below:

CAUTION

In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment.

Specifications

Voltage Rating Current Rating Temperature Rating Length Fittings Configuration

125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area) 6A minimum 60°C minimum

3.05 m maximum Grounding type attachment plug cap. Opposite terminates in molded-on IEC configuration appliance coupling.

Table 1 Certified Cord

A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in Table 1. In case you are unable to buy locally the power supply cord which is approved by one of the agencies mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in your country.

DP73

Country Agency

Argentina IRAM

Australia SAA Japan

Austria ÖVE Netherlands KEMA

Belgium CEBEC Norway NEMKO

Canada CSA Spain AEE

Denmark DEMKO Sweden SEMKO

Certification

Mark

Country Agency

Italy IMQ

JET, JQA, TÜV, UL Japan/METI

Certification

Mark

Country Agency

Certification

Mark

Country Agency

Certification

Mark

Finland FEI

France UTE

Germany VDE U.S.A. UL

Ireland NSAI

Switzerland SEV

United Kingdom

ASTA BSI

Table 2 HAR Flexible Cord

APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS

DP73

Printed or Embossed Harmoniza-

Approval Organization

Comite Electrotechnique Belge (CEBEC)

Verband Deutscher Elektrotechniker (VDE) e.V. Prüstelle

Union Technique de I´Electricite´ (UTE)

Instituto Italiano del Marchio di Qualita´ (IMQ)

British Approvals Service for Electric Cables (BASEC)

N.V. KEMA KEMA-KEUR <HAR> 10 30 30

SEMKO AB Svenska Elektriska Materielkontrollanstalter

tion Marking (May be located on jacket or insulation of internal wiring)

CEBEC <HAR> 10 30 10

<VDE> <HAR> 30 10 10

USE <HAR> 30 10 30

IEMMEQU <HAR> 10 30 50

BASEC <HAR> 10 10 30

SEMKO <HAR> 10 10 50

Alternative Marking Utilizing Black-Red-Yellow Thread (Length of color section in mm)

Black Red Yellow

Österreichischer Verband für Elektrotechnik (ÖVE)

<ÖVE> <HAR> 30 10 50

Danmarks Elektriske Materialkontroll (DEMKO)

National Standards Authority of Ireland (NSAI)

Norges Elektriske Materiellkontroll (NEMKO)

Asociacion Electrotecnica Y Electronica Espanola (AEE)

Hellenic Organization for Standardization (ELOT)

Instituto Portages da Qualidade (IPQ)

Schweizerischer Elektro Technischer Verein (SEV)

Elektriska Inspektoratet SETI <HAR> 10 30 90

Underwriters Laboratories Inc. (UL) SV, SVT, SJ or SJT, 3 X 18AWG Canadian Standards Association (CSA) SV, SVT, SJ or SJT, 3 X 18AWG

<DEMKO> <HAR> 30 10 30

<NSAI> <HAR> 30 30 50

NEMKO <HAR> 10 10 70

<UNED> <HAR> 30 10 70

ELOT <HAR> 30 30 70

np <HAR> 10 10 90

SEV <HAR> 10 30 90

MEMO

DP73

MEMO

DP73

2011 12 -02

Olympus DP73 User Manual [ru]

Specifications and Main Features

Frequently Asked Questions

User Manual