This instruction manual is for the Olympus Motorized System Microscopes Models BX61 and BX62.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of
this microscope, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future
reference.
A X 7 6 1 1
Page 2
Page 3
CONTENTS
Correct assembly and adjustments are indispensable to allow the microscope manifest its full performance. If you
are going to assemble the microscope by yourself, please read chapter 7, “ASSEMBLY” (pages. 29 to 32).
BX61
IMPORTANT — Be sure to read this section for safe use of the equipment. —
3-1 Base ................................................................................................................................................................................................................. 8-10
1 Voltage Indication2 Transmitted/Reflected Light Switch
3 Light Preset Switch4 Using the Filters
3-7 Objectives with Correction Collar ............................................................................................................................... 21
1-3
4-5
6-7
8-21
4 TROUBLESHOOTING GUIDE
5 SPECIFICATIONS
6 OPTICAL CHARACTERISTICS <<UIS2 (UIS) Series>>
ASSEMBLY
7
PROPER SELECTION OF THE POWER SUPPLY CORD...................................................................
—
See this section for the replacement of the light bulb. —
22-24
25-26
27-28
29-32
33-34
Page 4
This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive.
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the equipment.
Page 5
IMPORTANT
Each of these microscopes employs a UIS2/UIS (Universal Infinity System) optical design, and should
be used only with modules designed for the BX2 series (which belong to the Olympus BX series
microscopes) and objectives/eyepieces for the UIS series.
For the applicable module, please consult Olympus or the catalogues. Less than optimum performance
may result if inappropriate accessories are used.
Configuration of Instruction Manuals
Since these microscopes are expandable to a variety of systems, separate instruction manuals are provided
so that the user has to read only the manuals according to the user’s own system.
1. After the equipment has been used in an observation of a specimen that
is accompanied with a potential of infection, clean the parts coming in
contact with the specimen to prevent infection.
· Moving this product is accompanied with the risk of dropping the
specimen. Be sure to remove the specimen before moving this
product.
· In case the specimen is damaged by erroneous operation, promptly
take the infection prevention measures.
· The product becomes unstable if its height is increased by an accessory
mounted on it. In this case, take anti-toppling measures to prevent the
specimen from being dropped when the product topples down.
2. Install the microscope on a sturdy, level table or bench so as not to block
the air vents on the underside of the base.
Do not place the microscope on a flexible surface, as this could result in
blocking the air vents and cause overheating or a fire.
3. If a foreign object is caught during motorized focusing operation, there
will be an error in the focusing block and the motorized focusing operation will be suspended.
Recovery procedure
· If there is no error in motorized operation, the caught object can be
removed by turning the focusing knob.
· If there is an error in motorized operation, the focusing knob becomes
inoperable. Disassemble the relevant modules to remove the caught
object. Replace the relevant modules afterward.
· Turn off the power and then on again. The system will restart unless
there is a malfunction in the motor.
4. To activate temporary stop during focusing operation, turn the focusing
knob (or dial) on the microscope frame (in either direction) or press any of
the FOCUS control buttons ( , , F/C and ESC) (except during PC
communication).
When the main switch of the BX-UCB control box is set to “ I ” (ON), the
focusing operation starts automatically as part of initialization (this operation consists of temporary lowering and then returning to the original
position of the stage). If any of the operations mentioned above is performed, an emergency stop will also occur. In this case, set the main
switch to “ ” (OFF) and then “ I ” (ON) again.
1
Page 6
Fig. 1
²
@
5. When lowering the stage by pressing the stage DOWN button @, be
careful not to have your hand caught between the bottom ² of the condenser and the base.
6. The surfaces of the lamp housing on the rear of the microscope will
become extremely hot during operation. When installing the microscope,
make sure to allow ample free space (10 cm or more) around and in
particular above the lamp housing.
7. When installing the microscope, route the power cord away from the
lamp housing. Should the power cord come in contact with the hot lamp
housing, the power cord could melt and cause electric shock.
8. To avoid potential shock hazards and burns when replacing the light
bulb, set the main switch ³ to “ ” (OFF) then disconnect the power cord
from the wall outlet in advance. Whenever you replace the bulb during
use or right after use, allow the lamp housing | and bulb to cool before
touching. (Fig. 2)
|
³
Fig. 2
Designated
haiogen bulbs:
# The microscope also incorporate a fuse (this should be
replaced by the manufacturer or an authorized agent).
9. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
this instruction manual. If the proper power cord is not used, product
safety performance cannot be warranted.
10.
Always ensure that the grounding terminal of the microscope and that
of the wall outlet are properly connected. If the equipment is not grounded,
Olympus can no longer warrant the electrical safety performance of the
equipment.
11.
Never insert metallic objects into the air vents of the microscope frame
as this could result in electrical shock, personal injury and equipment
damage.
When using the U-FWR motorized filter wheel for reflected light observa-
12.
tion, remove the push ring driver from the position where it is attached by
magnetism in advance to prevent it from dropping.
If the driver drops in a certain place, an electric shock and/or burns may result.
12V100WHAL (PHILIPS 7724)
12V50WHAL-L (LIFE JC)
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner.
SymbolExplanation
2
Indicates that the surface becomes hot, and should not be touched with bare hands.
Before use, carefully read the instruction manual. Improper use could result in personal injury to
the user and/or damage to the equipment.
l
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Warnings
Warning engraving/stickers are placed at parts where special precaution is required when handling and using the
microscope. Always heed the warnings.
Warning engraving
position
Lamp housing
(Warning against high temperature)
Page 7
1 Getting Ready
Fig. 3
BX61
1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact.
2. Do not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, dust or vibrations. (For the operating conditions, refer to chapter 5, “SPECIFICATIONS”.)
3. When moving the microscope, remove the modules that may drop
including the specimen from the microscope in advance, hold it by the
hand hook on the rear and the base as shown on the left (Fig. 3) and
carry carefully (Weight: approx. 16 kg).
#Before transporting the microscope, be sure to remove the speci-
men, eyepieces and color glass filter so that they will not drop. Also
remove other modules attached to the microscope because they
increase the system weight.
# Damage to the microscope will occur if you grasp it by the stage,
focus adjustment knob or binocular section of the observation tube.
4. The BX61 series can be used with up to two intermediate attachments
including a U-CA magnification changer, U-EPA2 eyepoint adjuster, etc.
For restrictions when using two intermediate attachments, make sure to
read the instruction manuals provided with the respective intermediate
attachments.
2 Maintenance and Storage
1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and
wipe gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute
alcohol.
!Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical
equipment that is being switched on or off, which could cause ignition of a fire.
Also remember to always use it only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean
them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance.
4. When not using the microscope, set the main switch to “ ” (OFF), wait until the lamp housing has cooled down, and
cover the microscope with a dust cover before storage.
5. When disposing of the microscope, check the regulations and rules of your local government and be sure to observe
them.
3 Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The warning, caution and other notes in this manual use the following symbols.
!: Indicates that failure to follow the instructions in the warning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).
Intended use
4
This instrument has been designed to be used to observe magnified images of specimens in routine and research
applications. Do not use this instrument for any purpose other than its intended use.
3
Page 8
1
}If you have not yet assembled the microscope, read chapter 7, “ASSEMBLY” (pages 29 to 32).
} Make a photocopy of the observation procedure pages and post it near your microscope.
Š
and installation of the Z-board, refer to
page 30. For the information regarding
the AF-board, refer to the instruction
manual of the AF unit.
7
Page 12
USING THE CONTROLS
3-1 Base
ƒ
²
@
³
Fig. 4
Fig. 5
|
…
1 Voltage Indication
1. Press the Brightness adjustment button @ to increase the voltage and
make illumination brighter.
Pressing the button ² makes the illumination darker.
2. The numerals on the left of the lamp voltage LEDs ³ indicate the
approximate voltage.
#The LED may sometimes go out (temporarily) when the motor is
driving. Even in this case, there is no fluctuation in illumination
voltage.
2 Transmitted/Reflected Light Switch
The illumination can be switched between the transmitted light and reflected light by pressing the transmitted/reflected light switch |.
The indicator of the selected illumination lights.
3 Light Preset Switch
}The Light Preset switch sets the light intensity voltage to a voltage suit-
able for color photography* (the factory default is 9 V) regardless of the
current setting of the light intensity adjustment buttons.
* Achieved by engaging the built-in LBD filter.
Setting the Desired Brightness (Figs. 4 & 5)
1. Press the Light Preset switch ƒ The PHOTO indicator … lights up.
2. Press one of the light intensity adjustment buttons @ ² to set the desired brightness.
3. Press the Light Preset switch again. The indictor turns off, the brightness
returns to the original brightness but the setting made above is stored in
memory.
4. Hereafter, pressing the Light Preset switch sets the brightness to the intensity value set in step 2 above.
#Be sure to set the light intensity voltage back to 9 V when color
photography is to be performed.
}When the Light Preset switch ƒ is pressed, a note sound (short beep) is
generated at the 9 V position.
(Fig. 4)
(Fig. 4)
(Fig. 5)
8
4 Using the Filters
}You can place a filter in the light path with one of the following four
methods.
· Push in the knob of a built-in filter to engage it in the light path. (Page 9)
· Place a filter on the filter mount on the base and engage in the light path.
(Page 9)
· Insert a filter in the U-FC filter cassette, attach it on the filter mount and
engage it in the light path by sliding the filter lever. (Page 9)
· Insert a filter in the U-FWT filter wheel, attach it on the filter mount and
engage it in the light path by motorized operation (using the PC). (Refer
to the separate instruction manual.)
(Figs. 6 - 11)
Page 13
@
Fig. 6
²
³
|
Using Built-In Filters (Fig. 6)
Each filter can be engaged into the light path by pressing one of the filter
knobs @ to | in. Pressing the button again disengages the filter from the
light path.
Filter Type/Purpose
@
ND6 (Neutral Density filter for light adjustment, transmittance 6%)
²
ND25 (Neutral Density filter for light adjustment, transmittance 25%)
³LBD (for color balancing, daylight filter)
|OP (optional) filter set*
* Ask your Olympus representative to mount the filters.
BX61
ƒ
Fig. 7
Fig. 8
…
Mounting a Single Filter (Fig. 7)
A filter with a diameter of 45 mm ƒ can be placed on the filter mount on
the base. If you need to place multiple filters, please purchase the U-FC
filter cassette.
# Even when a filter cassette is used, a filter with thickness of no more
than 3 mm can still be placed on the filter mount.
Using the Filter Cassette (Figs. 8 - 11)
Loading Filters into Filter Cassette
}The filter cassette accommodates filters with a diameter of 45 mm and
thickness of 2.7 mm or less.
}The filter cassette has two filter levers on the right side and one on the left
side.
1. Move all filter levers to the OUT position except for the one belonging to
the slot into which the filter is to be inserted.
2. Slide the lever … to the IN position. Make sure that it clicks securely into
place. (Fig. 8)
3. Holding the lever in the position shown in Fig. 8, put the filter into the
cassette by inserting it in the direction indicated by the arrow.
4. Insert the other two filters in the same manner.
2. Align the key ‡ on the bottom surface of the filter cassette with the
positioning slot Š on the filter mount, then snap the filter cassette into
place from above.
3. Rotate the filter cassette to align its sides with the base. (Fig. 10)
4. Align the clamping screw † with the positioning hole ‰ on the filter
mount, then tighten the screw to fasten the filter cassette.
#When the filter cassette is installed, the condenser may hit the filter
cassette when the stage is lowered. Therefore, exercise caution
when lowering the stage with the filter cassette installed.
Fig. 11
‹
Using the Filter Cassette (Fig. 11)
Usable FiltersApplications
45ND-6, 45ND-25Neutral density filter
45G-530, 45G-533, 45IF550Green
45Y-48YellowB&W contrast filter
45O-560Orange
Up to three of the above filters can be loaded in the filter cassette. Moving the levers ‹ on the left and right sides of the cassette to the IN
position brings the corresponding filter into the light path.
10
Page 15
3-2 Focusing Block
²
@
³
Fig. 12
BX61
#If the substage is not installed in the correct position, it
may deviate from the coverage range of the damper spring
of the objective and damage the specimen.
Ensure that the top surface @ of the substage is flush with the bottom
surface of the notch ² on the mount dovetail. If they are not flush, loosen
the clamping screw ³ and lower the substage until it is stopped.
@
³
²
Fig. 13
@
|
1 Replacing the Focus Adjustment Knob
# The focus adjustment knob is installed on the right side of the micro-
scope when it left the factory.
}The focus adjustment knob is designed detachable to prevent interfer-
ence with hand during manipulation of the focus adjustment knob or X/
Y-axis knobs.
Usually attach the knob on the opposite side to the stage feed knobs.
1. Loosen the clamping screw @ and remove the focus adjustment knob
².
2. Remove the seal from the focus adjustment knob screw hole on the
other side and attach the knob by reversing the removal procedure.
3. Attach a provided seal on the screw hole | of the focus adjustment dial
³, from which the focus adjustment knob has been removed.
}The focus adjustment dial ³ can be operated with your fingertip or finger
surface at the same time as manipulating the X/Y-axis knobs.
2 F/C button
}This button switches the movement of the stage using the focusing
knob @ or dial between F (Fine) and C (Coarse). (For the purpose of
safety, this button is set automatically to F at the moment the main
switch of the BX-UCB control box is set to “ I ” (ON).)
· Each press of the F/C button ² switches F and C alternately.
(Fig. 13)
(Fig. 14)
²
Fig. 14
Stage Fine movement:0.1 mm per turn
Stage Coarse movement: 1 mm per turn
11
Page 16
²
@
Fig. 15
3 Stage UP/DOWN Buttons
!When lowering the stage, be careful not to have your hand caught
between the bottom of the condenser and the base.
· Press the stage UP button @ to raise the stage and press the stage
DOWN button ² to lower the stage.
· The stroke is 25 mm. It becomes 14 mm when the U-FWT filter wheel is
used.
· The upper limit of stage movement has been set so that the stage stops
within the coverage range of the damper spring of objective when a slide
specimen (thickness 1.2 mm) is used.
#When observing a metallurgic specimen with thickness above 1.2
mm, take care so that the stage does not hit the objective.
(Fig. 15)
@
Fig. 16
4 Stage Escape Button
When replacing the specimen, press the stage escape button @. The
stage will lower by 5 mm (in 1 sec.). Pressing the button again returns the
stage to the original height.
(Fig. 16)
12
Page 17
BX61
3-3 Stage
}The following description deals with biological specimens. When observing a metallurgic specimen, it is more convenient
to replace the special stage or slide holder with the stage plate. (For details, refer to the instruction manual for the BX-RLAA.)
²
Fig. 17
Fig. 18
@
1 Placing the Specimen
# The dimensions of the slide glass should be 26 x 76 mm with thick-
ness of 0.9 to 1.2 mm, and the cover glass should have thickness of
0.17 mm.
# When observing very large specimens, remove the slide holder and
place the specimen directly on the stage.
Microscopy with Double-Slide Holder (Fig. 17)
1. Press the stage DOWN button @ or stage escape button to lower the
stage.
2. Open the specimen holder clamping lever ² and place the specimen
slides on the stage by sliding each slide from the front.
3. After placing the slides as far as they will go, gently release the clamping
lever.
Microscopy with Single-Slide Holder (Fig. 18)
The specimen slide can easily be placed by sliding it into the slide holder
from the front.
Examining a whole specimen
Use one of the thin slide holders listed below that cause less interference
with the objectives*.
· U-HRD-4 · U-HLD-4· U-HLS-4
* The applicable objectives are ones are ones with 40X or less
magnification (except Apo series).
³
Using an Oil Immersion Objective
Adsorption of immersion oil can cause the specimen to drift. In such
cases, it is recommended to use the optional BH2-SCB-3 specimen clip
³ for oil immersion objectives. (Fig. 19)
Using an Oil Immersion Condenser
It may be effective to use the optional U-SVRO (right-hand knob) or USVLO (left-hand knob) slotted stage which prevents close contact between the specimen and stage.
Fig. 19
13
Page 18
2 Adjusting the X/Y-Axis Knob Tension
(Fig. 20)
²
|
³
@
Fig. 20
1. Hold the X-axis knob @ and slide up the Y-axis knob ² up to expose the
adjustment knobs.
2. Turning the X-axis adjustment knob ³ or Y-axis adjustment knob | clockwise (in the direction of the arrow) increases the rotation tension and
counterclockwise decreases it.
#If the tension is adjusted too tight, a creaking sound may be heard
during stage travel, and the stage stopping accuracy may be imperiled.
CAUTION
[Treatment] X-axis direction: Hold the specimen holder and move the
Rubber Stage Knob Caps (Optional)
}When the X/Y-axis knobs are fitted with the rubber caps, the knobs can
be adjusted without slipping and fine adjustment is possible by holding
the knobs with a very light force. The knob rubber caps also reduce
fatigue after long hours of operation.
The U-SHGT thick type (thickness 5 mm) and U-SHG thin type (thickness
2 mm) knob rubber caps are available.
To attach the knob rubber caps:
First fit the larger rubber knob cap to the Y-axis (upper) knob from below
it, then fit the smaller rubber knob cap to the X-axis (lower) knob from
below it.
After long hours of use, the stage guide may be deviated and the stage travel range may be decreased.
However, this is not malfunction and can be corrected
easily as described below.
stage guide to the left and right so that it hits
the stoppers.
Y-axis direction: Hold the upper stage and move it to the front
and rear so that it hits the stoppers.
14
@
Fig. 21
3 Rotating the Stage
1. Slightly loosen the stage clamping knob @.
2. The stage can be rotated both clockwise and counterclockwise while
holding the stage clamping knob.
#A click may be heard and felt during rotation. However, this is due to
the construction of the substage and does not indicate a malfunction.
}The angle of rotation varies depending on the positions of the feed knobs.
Angle of Rotation
ClockwiseCounterclockwise
Right-hand knobs230°20°
Left-hand knobs20°230°
(Fig. 21)
Page 19
BX61
²
Fig. 22
|
Fig. 23
@
ƒ
³
4 Adjusting the Stage Height
}By lowering the position of the substage, the microscope will accommo-
date specimens with maximum height of 35 mm. This is useful when
observing metallurgical specimens and other thick objects.
1. Press the stage DOWN button @ to lower the stage to the lower limit,
then remove the stage from the microscope.
2. Using an Allen screwdriver, loosen the substage bracket clamping screw
² and remove the substage.
3. Press the stage UP button ³ and raise the focusing block ƒ to where
the stopper screw | on the arm becomes visible.
4. Using the Allen screwdriver, loosen and remove the upper stopper screw
|.
5. Reattach substage bracket and stage.
}Retain the removed stopper screw | and take care not to lose it.
(Figs. 22 & 23)
15
Page 20
3-4 Observation Tube
²
Fig. 24
Fig. 25
@
1 Adjusting the Interpupillar Distance
While looking through the eyepieces, adjust for binocular vision until the
left and right fields of view coincide completely. The index dot · indicates
the interpupillary distance.
}Note your interpupillary distance so that it can be quickly duplicated.
2 Adjusting the Diopter
1. Looking through the eyepiece without the diopter adjustment ring, rotate
the coarse and fine focus adjustment knob to bring the specimen into
focus.
2. Looking through the eyepiece with the diopter adjustment ring @, turn it
to focus on the specimen. (Fig. 25)
(Figs 25 & 26)
(Fig. 24)
Fig. 26
Using a Finder Eyepiece
1. Looking through the right eyepiece with your right eye, turn the top of the
eyepiece ² until a clearly defined double crossline can be seen in the
field of view. (Figs. 25 & 26)
2. Looking through the right eyepiece, rotate the coarse and fine focus adjustment knob to bring the specimen and double crossline into simultaneous focus.
3. Looking through the left eyepiece with your left eye, turn the diopter adjustment ring @ to focus on the specimen.
Using an Super Widefield Observation Tube
The operation is similar to the above. However, as the super widefield
tube does not have the diopter adjustment ring on the left sleeve, focus
on the specimen by turning the top of the left sleeve.
3 Using the Eye Shades
When Wearing Eyeglasses
Use with the eye shades in the normal, folded-down position. This will
prevent the eyeglasses from being scratched.
When Not Wearing Eyeglasses
Extend the folded eye shades in the direction of the arrow to prevent
extraneous light from entering between the eyepieces and eyes.
(Fig. 27)
16
Fig. 27
Page 21
BX61
Fig. 28
Fig. 29
@
@
²
4 Using the Eyepiece Micrometer Disk
When the WHN10X-H (or WHN10X) eyepieces are used, an eyepiece
micrometer disk can be inserted in one of them. When the eyepiece
does not have a diopter adjustment mechanism, however, it is hard to
focus on the micrometer disk if the operator has poor eyesight. Should
that be the case, adjust the focus with eyeglasses on.
Use 24 mm dia. x 1.5 mm thick micrometer disks.
Following Fig. 28, turn the built-in micrometer-mounting frame ²
counterclockwise to remove it from the eyepiece and place a micrometer
disk.
Screw the micrometer mounting frame back into the eyepiece sleeve.
Selecting the Light Path of the
5
Trinocular Tube
Slide the light path selector knob @ to select the desired light path.
Trinocular tube
U-TR30-2
U-SWTR-3
U-TR30NIR
*
100% for binocular eyepieces
Light path selector knob position
Pushed in
Middle position
20% for binocular
eyepieces, 80% for
TV/photography
50% for binocular
eyepieces, 50% for
TV/photography
100% for TV/
photography
(Fig. 28)
(Fig. 29)
Pulled out
* With the infrared trinocular tube, infrared observation up to 1000 nm is
possible. For details, consult your Olympus representative.
17
Page 22
Fig. 30
6 Adjusting the Tilt (with the U-TBI3)
}Adjust the height and tilt of the observation tube to obtain the most
comfortable viewing position.
Holding the binocular section with both hands, raise or lower it to the
desired position.
# Never attempt to force the binocular section past the upper or lower
stop position. Applying excessive force could destroy the limiting
mechanism.
}The U-TBI3 can be used in combination with only one intermediate at-
tachment. However, no intermediate attachment can be combined when
the BX-RFAA or BX-RLAA reflected light illuminator is used.
}If you need photography using the U-TBI3, also use the U-TRU trinocular
intermediate tube.
With the U-ETBI/U-TTBI (Fig. 31)
The U-ETBI and U-TTBI are ergonomic observation tubes with normal
field, capable of the tilting adjustment as well as the adjustment of the
eyepiece position toward the front and rear (by 45 mm). The U-ETBI is the
erect image model and the U-TTBI is the inverted image model, and
both models are of the same size.
#The intermediate attachments that can be combined with the U-
TTBI are limited. For details, please contact Olympus.
(Fig. 30)
Fig. 31
18
Page 23
3-5 Condenser
BX61
@
²
Fig. 32
³
1 Centering the Condenser
1. Fit the Allen screwdriver @ into the condenser height adjustment screw
and turn the tool counterclockwise to raise the condenser to its upper
limit.
2. Focus on the specimen using the 10X objective.
# When using a swing-out condenser, move the top lens into the light
path.
3. Rotate the field iris diaphragm ring ² in the direction of the arrow so that
the diaphragm image comes inside the field of view.
4. Turn the Allen screwdriver @ to focus on the diaphragm image.
5. Fit the Allen screwdrivers into the two condenser centering screws ³
and turn them to move the iris diaphragm image to the center of the field
of view.
6. Gradually open the field iris diaphragm. The condenser is properly centered if the iris image is centered and inscribed in the field of view.
7. During actual use, open the field diaphragm slightly until its image circumscribes the field of view.
(Figs. 32 & 33)
Fig. 33
Effects of Field Iris Diaphragm (Fig. 33)
The field iris diaphragm restricts the diameter of the beam of light entering the objective and thus excludes extraneous light, improving image
contrast. The diameter of the field iris should e adjusted for objective
power to the extent that it just circumscribes the field of view. (See “Compatibility of Objectives and Condensers” on the next page.)
19
Page 24
Aperture iris
diaphragm image
Objective pupil
Fig. 34
70-80%
30-20%
²
Aperture Iris Diaphragm (Figs. 34 & 35)
· The aperture iris diaphragm determines the numerical aperture of the
illumination system. It has an effect of adjusting image resolution and
contrast. Stopping down the aperture iris diaphragm increases the depth
of focus.
· Since the contrast of microscope specimens is ordinarily low, setting the
condenser aperture iris diaphragm to between 70 and 80% of the N.A. ²
of the objective in use is usually recommended.
Adjust the ratio by removing the eyepiece and looking into the eyepiece
sleeve while adjusting the aperture iris diaphragm ring @ until the image
shown in Fig. 34 is seen.
}Using the numerical aperture scale:
You can use the condenser numerical aperture scale by adjusting the
aperture iris diaphragm ring @. (Fig. 35)
@
Fig. 35
N.A. scale
2 Compatibility of Objectives and Condensers
Objective
Magnification
1.25XApplicable to FN 22
2X
4X
10-60X
100X
* When using the U-SC3 swing-out condenser together with a 1.25X to 4X objective, fully open the condenser aperture iris
diaphragm and use the field iris diaphragm in the base as aperture diaphragm. With the 1.25X to 2X objectives, the
surroundings of the field of view may become dark.
}To obtain better illumination in photomicrography using a 1.25X to 4X objective, use of the U-ULC-2 for ultralow magnifi-
cation is recommended.
Achromat/aplanat
U-AAC
Applicable
(FN 26.5)
Applicable (FN 26.5) by moving
top lens out of the light path.*
Engage top lens in light path.
Condenser
Swing-out
U-SC3
(FN 26.5)
Motorized universal
U-UCD8A
Applicable when U-FWCO1.25X
is combined.
Applicable (FN 26.5) by moving
top lens out of the light path.
Engage top lens in light path.
(FN 26.5)
20
Page 25
3-6 Immersion Objectives
# Be sure to use the provided Olympus Immersion oil.
BX61
Fig. 36
1 Using Immersion Objectives
1. Focus on the specimen using all objectives, starting from the lowestpower objective to higher-power objectives.
2. Press the stage escape button to lower the stage, then place a drop of
provided immersion oil onto the specimen at the area to be observed.
3. Turn the revolving nosepiece to engage the immersion objective.
4. Press the stage escape button to raise the stage, then turn the focus
adjustment knob to focus on the specimen.
# Since air bubbles in the oil will affect the image quality, make sure
that the oil is free of bubbles.
a. To check for bubbles, remove the eyepiece and fully open the field and
aperture iris diaphragms, then look at the exit pupil of the objective inside
the observation tube. (The pupil should appear round and bright.)
b. To remove bubbles, turn the revolving nosepiece toward the shorter ob-
jectives and repeatedly defocus and refocus the oil immersion objective.
}If the condenser engraving shows a numerical aperture (NA) of 1.0 or
higher, the number applies only when oil is applied between the slide
glass and the top surface of the condenser. When oil is not present, the
NA is about 0.9.
5. After use, remove oil from the objective front lens by wiping with gauze
slightly moistened with absolute alcohol.
!Caution in use of immersion oil
If immersion oil enters your eyes or contacts with your skin, immediately
take the following treatment.
Eyes: Rinse with fresh water (for 15 minutes or more).
Skin: Rise with water and soap.
If the appearance of the eyes or skin is altered or pain persists, immediately see your doctor.
(Fig. 36)
3-7 Objectives with Correction Collar
}If the cover glass thickness is not 0.17 mm, the objectives cannot mani-
fest their performances. If a correction collar equipped objective is used
in this case, the difference in thickness can be compensated for by adjusting the collar.
Adjustment Procedure
· If the cover glass thickness is known, set the correction collar @ to that
@
Fig. 37
value. (Fig. 37)
· If the cover glass thickness is unknown, adjust the correction collar @
and focus adjustment knob alternately until the positioning with the highest
resolution is obtained.
# Be careful not to touch the correction collar @ when turning the
revolving nosepiece.
21
Page 26
TROUBLESHOOTING GUIDE
Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur,
please review the following list and take remedial action as needed. If you cannot solve the problem after checking the
entire list, please contact your local Olympus representative for assistance.
Problem
1. Optical System
a) Bulb does not light.Power cord of BX-UCB is unplugged.
Main switch of BX-UCB is not on.Set main switch to “ I ” (ON).–
Lamp ON-OFF switch on the base of
BX61 is not on.
Bulb is burned out.Replace bulb with a new specified
Transmitted/reflected light switch is set
to reflected light ( ).
b) Bulb operates, but field of view re-
mains dark.
c) Field of view is obscured or not
evenly illuminated
Aperture and field iris diaphragms are not
opened wide enough.
Condenser is lowered too much.Adjust the condenser height position.19
Light path selector knob is set to the
position.
Light path selector knob is in an positioned halfway.
Revolving nosepiece is not correctly engaged.
Condenser is not attached properly.Re-attach it.32
Revolving nosepiece is not attached
properly.
An objective that falls outside of the
condenser’s illumination range is used.
Condenser is not properly centered.Center the condenser.19
CauseRemedyPage
Insert power plug firmly into power
outlet.
Set the lamp ON-OFF switch to ON.
bulb.
Set the switch to (transmitted light
illumination).
Adjust them to proper sizes.
Move the knob to the or position.
Set the knob according to the observation method.
Make sure that the revolving nosepiece clicks properly into place.
Push the side dovetail all the way until it is stopped.
Use a condenser to match the purpose.
–
–
31
8
19/20
17
17
–
–
20
22
d) Dirt or dust is visible in the field of
view.
e) Visibility is poor.
· Image is not sharp.
· Contrast is poor.
· Details are indistinct.
· Image glares.
Field iris diaphragm is stopped down too
far.
Bulb is not mounted correctly.Push the pins of halogen bulb all the
Dirt/dust on the eyepieceClean thoroughly.
Dirt or the to surface of the condenser
Dirt/dust on the specimen
A non-UIS2/UIS objective is used.Use only UIS2/UIS series objectives
Condenser is lowered too far.Adjust the condenser height position.19
Aperture iris diaphragm is stopped down
too far.
Revolving nosepiece is not mounted
properly.
Open the field iris diaphragm until it
circumscribes the field.
way until the stop position.
with this microscope.
Open aperture iris diaphragm.
Push the slide dovetail all the way until
it is stopped.
19
31
27/28
20
3
–
Page 27
BX61
Problem
e) Visibility is poor.
· Image is not sharp.
· Contrast is poor.
· Details are indistinct.
· Image glares.
f ) One side of image is blurred.Revolving nosepiece is not correctly
g) Image appears to waver.Revolving nosepiece is not corrected
Correction collar on correction collar
equipped objective is not properly adjusted.
Front lens of objective is dirty.Clean objective.3
Immersion oil is not being used with an
oil immersion objective.
Immersion oil contains bubbles.Remove the bubbles.21
Recommended immersion oil is not
used.
Dirt/dust on specimen.Clean it.
Dirt/dust on condenser
Inappropriate object side or cover glass
thickness.
mounted.
Stage is not correctly mounted.Re-attach it.15
Objective is not correctly engaged in light
path.
Specimen is not correctly mounted on
stage.
mounted.
Objective is not correctly engaged in
light path.
Condenser is not properly centered.Center the condenser.19
CauseRemedyPage
While focusing, turn the correction collar to find the best position.
Use immersion oil.
Use the provided immersion oil.
Replace with glass of recommended
thickness.
Push slide dovetail all the way until it
is stopped.
Make sure that revolving nosepiece
clicks into place correctly.
Place specimen correctly on to of
stage and secure it with slide holder.
Push slide dovetail all the way until it
is stopped.
Make sure that revolving nosepiece
clicks into place correctly.
21
21
21
3
13
–
–
13
–
–
h) Field of view becomes only slightly
brighter when the voltage is raised.
2. Electrical System
a) Bulb intermittently lights and goes
out.
b) Bulb burns out almost immediately. Wrong type of bulb is being used.Use correct bulb type.31
c) All voltage indicator LEDs light and
voltage cannot be varied by pressing light intensity adjustment button.
d) Brightness does not change when
you press light intensity adjustment
button.
3. Focusing Block
a) Image cannot be focused.When adjusting stage height, you forgot
b) Stage rises too high and destroys
specimen.
Condenser is not properly centered.Center the condenser.19
Condenser is lowered too far.Adjust the condenser height position.19
Bulb is nearly burned out.Replace bulb.31
A connector is not properly connected.
Bulb is not installed.Install bulb.31
Bulb is burned out.Replace bulb.31
Lamp housing is not connected.Connect lamp housing correctly.–
Bulb is burned out.Replace bulb.
to reattach upper stopper screw.
Substage is not installed properly.Re-attach substage.
Check all connectors.
Re-attach upper stopper screw.
31
15
11
–
23
Page 28
Problem
c) Coarse adjustment will not go all
the way down.
d) Objective makes contact with speci-
men before focus is obtained.
4. Observation Tube
CauseRemedyPage
Condenser holder is too low.Raise condenser holder.
Specimen is mounted upside down.
Mount specimen correctly.
32
–
a) Field of view of one eye does not
match that of the other.
5. Stage
a) Image shifts when you touch stage. Stage is not properly mounted.Clamp stage.–
b) Specimen stops midway on the X-
axis traverse.
c) X-axis and/or Y-axis knobs are too
tight or too loose.
d) Stroke is decreased.Stage guide is deviated.Correct deviation as instructed on
Interpupillary distance is incorrect.Adjust interpupillary distance.16
Incorrect diopter adjustment.Adjust diopter.16
Different eyepieces are used on left and
right.
Your view is not accustomed to microscope observation.
Specimen is not correctly positioned.Place specimen correctly.
X-axis and/or Y-axis adjustment knobs
are set at a position where tension is too
high or too low.
Change on eyepiece to match the
other so that both sides are the same
type.
Upon looking into eyepieces, try looking at overall field before concentrating on specimen range. You may also
find it helpful to look up and into distance for a moment before looking
back into microscope.
3. Focusing mechanismDrive system: Motorized focusing using a stepping motor and ball screw.
Built-in transmitted Koehler illumination
12 V , 100 W long-life halogen bulb (pre-centered) 12 V 100 W HAL-L (PHILIPS 7724) or 12 V ,
50 W long-life halogen bulb (pre-centered) 12 V 50 W HAL-L (LIFE JC)
(Average life time: Approx. 2,000 hr. when used as directed)
Light intensity voltage range: 2 V or less to 12.0 V (continuous)
With a light intensity preset switch (voltage variation range: 2 V or less to 12.0 V )
Rated voltage of external power supply (BX-UCB): 100-120/220-240 V , 50/60 Hz, 3.5 A /1.5 A
Power consumption (BX-UCB):50–300 W (variable with number of connected modules)
(Ex.) Approx. 200 W with 100 W halogen bulb and motorized
focusing unit
Stage height movement by cross roller guides.
Finest adjustment scale: 1 μm (fine movement sensitivity 1 μm)
Resolution: 0.01 μm
Highest stage speed: 3 mm/sec.
Stroke per rotation: 0.1 mm (fine), 1 mm (coarse)
Full stroke range: 25 mm to 14 mm (when the U-FWT filter wheel is used)
· Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly
through 70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at 40°C (104°F).
· Supply voltage fluctuations; Not to exceed ±10% of the normal voltage.
· Pollution degree: 2 (in accordance with IEC60664)
· Installation/Overvoltage category: II (in accordance with IEC60664)
26
Page 31
BX61
OPTICAL CHARACTERISTICS «UIS2 (UIS) Series»
UIS series objectives not listed here can also be combined with this microscope.
The following table shows the optical characteristics of combinations of eyepieces and objectives. The figure on the right
shows the performance data engraved on the objectives.
NOTE
Refer to the latest catalogue or consult your local Olympus
representative for the updated information on the eyepieces
and objectives that can be combined with this microscope.
The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of assembly.
The BX61TRF microscope frame is assembled in the same procedures.
The module model numbers shown in the following diagram are merely the typical examples. For the modules with which
the model numbers are not given, please consult your Olympus representative or the catalogues.
# When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching any parts
or touching glass surfaces.
Assembly steps enclosed in will be detailed on the subsequent pages.
}Most assembly operations are possible by using the Allen screwdriver ( ) provided with the microscope. How-
ever, the assembly of the reflected light illuminator requires the use of the provided Allen wrench ( ) for clamping the
internal screws (to ensure the performance, we recommend that you have your Olympus representative assemble or
remove this module).
}For the electrical connections, refer to the instruction manual of the BX-UCB control box.
For the setup and installation of the Z-board, see page 30.
100 W halogen bulb
12V100WHAL-L
12V50WHAL-L
29
Page 34
7-2 Detailed Assembly Procedures
# For use in focusing control, only either the Z-board or AF control board can be installed on the BX-UCB.
Installing different boards on it may result in malfunction. When using the AF control board instead of the Z-board,
set the on-board DIP switches on the AF control board to the same settings as the following settings that are
originally intended for the Z-board.
Setup and Installation of the Z-Board
}The on-board DIP switches on the Z-board have been set up for the
BX61 or BX62 (all of switches S1, S2 and S3 are set to OFF).
²
S1S2S3
Fig. 38
@
Fig. 39
³
Changing the Settings of On-Board DIP Switches (Fig. 38)
#Leave all other DIP switches than those mentioned below in the OFF
positions.
}Switch only the DIP switches mentioned below to the ON positions.
S2 No. 4
· This replaces the functions of the F/C switching and stage escape buttons on the left side of the BX61/BX62 with the stage DOWN and UP
buttons.
S3 No. 1
· This turns off the automatic initialization and sets the remote mode which
is the default.
S3 No. 4
· When the U-FWT filter wheel is not used, this changes the focus adjustment stroke from 15 mm to 25 mm.
}When the U-LWUCD long-WD universal condenser is used, set this to
the OFF position.
Mounting the Z-Board (Fig. 39)
}Be sure to set the main switch on the BX-UCB control box @ to “ ”
(OFF) before proceeding to mounting the Z-board.
1. Loosen the 6 clamping knobs on the 2 option slot covers on the rear of
the BX-UCB and remove the covers and knobs.
2. Insert the Z-board ² into the BX-UCB by aligning the orientation of the
connector inside the BX-UCB and that of the Z-board. Push in the board
along the board rails all the way so that the connector is plugged in
securely.
3. Clamp the Z-board ² using two of the clamping knobs removed above.
Also attach one of the removed covers ³ using the other four clamping
knobs removed above.
}Store the other, removed cover in a safe place.
30
Page 35
BX61
@
²
Fig. 40
Fig. 41
ƒ
³
…
1 Installing the Halogen Bulb
}Use only the designated bulb 12V100WHAL-L (PHILIPS 7724) or
12V50WHAL-L (LIFE JC).
1. Fully loosen the lamp housing clamping screw @ on top of the lamp
housing cover with the provided Allen screwdriver.
2. Lift the lamp housing cover ² upward to remove it.
3. Turn the lamp socket ³ by 90° in the direction indicated by the arrow.
4. Holding the bulb ƒ with gloves or a piece of gauze, depress the bulb
clamping lever | and insert the bulb pins … fully into the sections † as
far as they will go.
Gently release the bulb clamping lever to the original position to secure
the bulb.
To prevent reduced bulb life or cracking, do not touch the bulb with
bare hands. If fingerprints are accidentally left on the bulb, wipe the
bulb with a soft cloth.
5. Side the lamp housing cover onto the housing base from the above.
Tighten the clamping screw @ while pressing downward on the cover.
(Fig. 40)
Caution for Bulb Replacement During Use or Right After Use
The bulb, lamp socket and areas near these will be extremely hot
during and right after use.
Set the main switch to “ ” (OFF), disconnect the power cord from
the wall outlet, then allow the old bulb and lamp housing to cool
before replacing the bulb with a new of the designated type.
(Figs. 40 - 42)
|
Fig. 42
Fig. 43
†
@
²
6 Attaching the Eyepiece
Fit the eyepiece into each sleeve as far as it goes.
# When using the U-BI30-2 binocular tube, eyepiece with built-in mi-
crometer disk cannot be attached.
# When using a finder eyepiece or an eyepiece with focusing mirror,
attach it to the right-hand eyepiece sleeve.
When doing so, make sure that the eyepiece positioning pin @ fits
into the notch ² on the eyepiece sleeve.
# The super-widefield trinocular observation tube is equipped with a
positioning notch on both eyepiece sleeves. Make sure that the
positioning pins on both eyepieces fits into the respective notches.
(Fig. 43)
31
Page 36
Fig. 44
³
²
@
9 Attaching the Condenser
}This operation necessitates the operation of the motorized focusing. Con-
nect the cables and set the main switch to “ I ” (ON) before proceeding to
the following.
1. Press the stage UP button @ to raise the stage to its upper limit.
2. Fit the Allen screwdriver into the condenser height adjustment screw ²
and turn the tool counterclockwise to lower the condenser to its lower
limit.
3. Fully loosen the condenser clamping screw ³ using the Allen screwdriver.
}When attaching the U-SC3 swing-out condenser, align the positioning
pin at the back of the condenser with the groove in the condenser sleeve.
#When attaching a condenser with swing-out top lens, swing out the
top lens out of the way before inserting the condenser.
4. Position the condenser so that the aperture scale or optical device indication plate is located in front. Gently slide the condenser along the
dovetail all the way until it is stopped.
5. Tighten the condenser clamping screw ³ and gently raise the condenser
holder to its highest position.
(Fig. 44)
32
Page 37
BX61
PROPER SELECTION OF THE POWER SUPPLY CORD
If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and
“ Certified Cord ” below:
CAUTION:
In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the
electrical safety of the equipment.
Specifications
Voltage Rating
Current Rating
Temperature Rating
Length
Fittings Configuration
125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)
6A minimum
60°C minimum
3.05 m maximum
Grounding type attachment plug cap. Opposite terminates in molded-on IEC configuration appliance coupling.
Table 1 Certified Cord
A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an
agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in
Table 1. In case you are unable to buy locally in your country the power supply cord which is approved by one of the
agencies mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in
your country.
CountryAgency
Argentina
Australia
IRAM
SAA
Certification
Mark
CountryAgency
Italy
Japan
IMQ
JET, JQA, TÜV,
UL-APEX / MITI
Certification
Mark
Austria
Belgium
Canada
Denmark
Finland
France
Germany
Ireland
ÖVE
CEBEC
CSA
DEMKO
FEI
UTE
VDE
NSAI
Netherlands
Norway
Spain
Sweden
Switzerland
United
Kingdom
U.S.A.
KEMA
NEMKO
AEE
SEMKO
SEV
ASTA
BSI
UL
33
Page 38
Table 2 HAR Flexible Cord
APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS
Approval Organization
Comite Electrotechnique Belge
(CEBEC)
Verband Deutscher Elektrotechniker
(VDE) e.V. Prüfstelle
Union Technique de l´Electricite´
(UTE)
Instituto Italiano del Marchio di
Qualita´ (IMQ)
British Approvals Service for Electric
Cables (BASEC)
N.V. KEMA
SEMKO AB Svenska Elektriska
Materielkontrollanstalter
Österreichischer Verband für
Elektrotechnik (ÖVE)
Danmarks Elektriske Materialkontroll
(DEMKO)
Printed or Embossed Harmonization Marking (May be located on
jacket or insulation of internal wiring)
CEBEC<HAR>
<VDE><HAR>
USE<HAR>
IEMMEQU<HAR>
BASEC<HAR>
KEMA-KEUR<HAR>
SEMKO<HAR>
<ÖVE><HAR>
<DEMKO><HAR>
Alternative Marking Utilizing
Black-Red-Yellow Thread (Length
of color section in mm)
BlackRedYellow
103010
301010
301030
103050
10103 0
103030
10105 0
301050
301030
National Standards Authority of Ireland
(NSAI)
Norges Elektriske Materiellkontroll
(NEMKO)
Asociacion Electrotecnica Y
Electronica Espanola (AEE)
Hellenic Organization for
Standardization (ELOT)
Instituto Portages da Qualidade
(IPQ)
Schweizerischer Elektro
Technischer Verein (SEV)
Elektriska Inspektoratet
Underwriters Laboratories Inc. (UL)SV, SVT, SJ or SJT, 3 X 18AWG
Canadian Standards Association (CSA)SV, SVT, SJ or SJT, 3 X 18AWG
<NSAI><HAR>
NEMKO<HAR>
<UNED><HAR>
ELOT<HAR>
np<HAR>
SEV<HAR>
SETI<HAR>
303050
101070
301070
303070
10109 0
103090
103090
34
Page 39
Page 40
ECREP
Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan
Wendenstraße 14-18, 20097 Hamburg, Germany
3500 Corporate Parkway, P.O. Box 610, Center Valley, PA 18034-0610, U.S.A.
One Corporate Drive, Orangeburg, NY 10962, U.S.A.
491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373
31 Gilby Road, Mount Waverley, VIC., 3149, Australia
Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.
5301
01/10
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