Leica ST4040 User Manual
Instruction Manual
Leica ST4040 V1.4 - Rev A, English – 06/2009
Always keep this manual near the instrument.
Read carefully prior to operating the instrument.
Leica ST4040
Linear Stainer
IMPORTANT NOTE
The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge
and state-of-the-art technology as we understand it following thorough investigation in this
field. We are under no obligation to update the
present manual periodically and on an ongoing
basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we
exclude liability as far as permissible according
to the national legal system applicable in each
individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to
compliance with statements or other information in this manual.
Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our prod-
ucts. These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is it
possible to continuously improve the technology and manufacturing techniques used in our
products.
This document is protected under copyright
laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
Issued by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: +49 6224 143-0
Fax: +49 6224 143-268
Internet: http://www.microsystems.com
Leica ST4040 – Linear Stainer
Serial No.: .............. ......................................................
Year of Manufacture: ...............................................
Country of Origin: Federal Republic of Germany
3
Table of Contents
IMPORTANT NOTE ........................................................................................................................................................................................... 3
1. Important Information .......................................................................................................................................................................... 6
1.1 Symbols used in this manual and ....................................................................................................................................................... 6
1.2 Designated use ...................................................................................................................................................................................... 7
2. Safety ....................................................................................................................................................................................................... 8
2.1 Safety instructions ................................................................................................................................................................................ 8
2.1.1 Transport and installation ................................................................................................................................................................... 8
2.1.2 Operating the instrument ..................................................................................................................................................................... 9
2.1.3 Cleaning and maintenance ............................................................................................................................................................... 10
3. Installation ........................................................................................................................................................................................... 11
3.1 Site requirements ................................................................................................................................................................................ 11
3.2 Unpacking the instrument .................................................................................................................................................................. 12
3.2.1 Repacking the instrument ................................................................................................................................................................. 12
3.3 Installing the instrument ..................................................................................................................................................................... 13
3.4 Standard delivery ‘basic instrument’ and general accessories ................................................................................................. 14
3.4.1 Standard delivery ................................................................................................................................................................................ 14
Basic instrument ST4040 - single load model ................................................................................................................................ 14
Basic instrument ST4040 - double load model (for double row staining) ................................................................................. 14
3.4.2 General accessories .......................................................................................................................................................................... 15
3.5 Electrical connections ........................................................................................................................................................................ 16
3.5.1 Adjusting the voltage selector .......................................................................................................................................................... 16
3.5.2 Connecting the power chord to the ................................................................................................................................................. 17
3.6 Installing the accessories .................................................................................................................................................................. 17
3.6.1 Installing the water inlet hose .......................................................................................................................................................... 17
3.6.2 Installing the drain hose .................................................................................................................................................................... 17
3.6.3 Installing the exhaust air hose ...........................................................................................
3.6.4 ..................................................................................................................................................................... Placing the instrument in its
........................................................................................................................................................................................................................
..................................................................................................................................................................................... permanent position
18
3.6.5 Connecting the inlet hose to the tap................................................................................................................................................ 18
3.6.6 Leveling the instrument ..................................................................................................................................................................... 18
3.6.7 Inserting the activated carbon filter ................................................................................................................................................ 19
3.7 Plugging the power chord into
...................................................................................................................................................................................................... the wall outlet
19
.............................................................. 17
4. Installation of the Optional Load/Unload Station ......................................................................................................................... 20
4.1 Site requirements ................................................................................................................................................................................ 20
4.2 Unpacking the optional load / unload station ....................................................................................................................... 20
4.2.1 Repacking the optional load / unload station ...................................................................................................................... 20
4.3 Standard delivery and accessories - optional load / unload station .......................................................................................... 21
4.3.1 Standard delivery - optional load station ......................................................................................................................................... 21
Accessories - optional load station ................................................................................................................................................. 21
4.3.2 Standard delivery - optional unload station ................................................................................................................................... 21
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Instruction Manual V 1.4 – 06/2009
Table of Contents
Accessories - optional unload station ............................................................................................................................................ 21
4.4 Installation ............................................................................................................................................................................................ 22
4.4.1 Installing the optional load station ................................................................................................................................................... 22
4.4.2 Installing the optional unload station ..................................................................................................................................................
on the right of the basic instrument ..................................................................................................................................................... 25
4.5 Changing the guide ramps for slide rack carriers ......................................................................................................................... 28
5. Instrument Properties ........................................................................................................................................................................ 29
5.1 Technical Data ..................................................................................................................................................................................... 29
6. Operation .............................................................................................................................................................................................. 30
6.1 Setting up the instrument ................................................................................................................................................................... 30
6.2 Preparing the instrument for staining: inserting and filling the stations .................................................................................... 30
6.3 Staining in two rows ........................................................................................................................................................................... 31
6.3.1 Doubling the specimen throughput ................................................................................................................................................. 31
6.3.2 Carrying out two different, but matching, staining protocols simultaneously ......................................................................... 31
6.3.3 Carrying out two different staining protocols (two subsequent single loads) ......................................................................... 31
6.4 Control panel functions ...................................................................................................................................................................... 32
6.5 Switching on mains and selecting direction of travel and alarm volume.................................................................................. 33
........................................................................................................................................................................................................................... 33
6.5.1 Selecting direction of travel .............................................................................................................................................................. 33
........................................................................................................................................................................................................................... 33
6.5.2 Selecting alarm volume ..................................................................................................................................................................... 34
........................................................................................................................................................................................................................... 34
6.6 Switching on mains without selecting direction of travel/alarm volume .................................................................................. 34
........................................................................................................................................................................................................................... 34
6.6.1 Stand-by mode .................................................................................................................................................................................... 34
........................................................................................................................................................................................................................... 34
6.7 Programming .................................................................................................................
6.8 Staining ................................................................................................................................................................................................. 36
6.8.1 Selecting a program ........................................................................................................................................................................... 36
........................................................................................................................................................................................................................... 36
6.8.2 Preparing and inserting the slide racks .......................................................................................................................................... 37
6.8.3 Starting the selected program .......................................................................................................................................................... 38
........................................................................................................................................................................................................................... 38
6.9 Removing the slide racks ................................................................................................................................................................... 39
6.8.4 Interrupting a program ....................................................................................................................................................................... 39
........................................................................................................................................................................................................................... 39
6.9.1 Removing the last slide rack ............................................................................................................................................................. 40
........................................................................................................................................................................................................................... 40
6.10 Removing the slide racks in instruments equipped with optional unload station ................................................................... 40
........................................................................................................................................................................................................................... 40
6.11 Finishing work ..................................................................................................................................................................................... 41
....................................................................... 35
........................................................................................................................................................................................................................... 41
7. Trouble Shooting ................................................................................................................................................................................. 42
8. Cleaning and Maintenance .............................................................................................................................................................. 44
8.1 Cleaning ................................................................................................................................................................................................ 44
8.2 Maintenance ........................................................................................................................................................................................ 46
Leica ST4040 – Linear Stainer
9. Warranty and Service ........................................................................................................................................................................ 47
5
1. Important Information
The instruction manual for the Leica
ST4040 includes chapters on the following
subjects:
Chapter 1 Structure of this manual
• Table of contents
• Important information on this
manual
Chapter 2 Safety
• Read this chapter before
operating the instrument
Chapter 3 Installation
• Unpacking and installation
• Standard delivery, accessory
range
Chapter 4 Installation
Optional load / unload station
• Unpacking and installation
• Standard delivery, accessory
range
Chapter 5 Instrument features
• Technical data
Chapter 6 Operation
• Controls
• Setup and daily use
Chapter 7 Trouble Shooting
• Operating errors
• Trouble shooting
Chapter 8 Cleaning and Maintenance
Chapter 9 Warranty and Service
Chapter 10 EC Declaration of Conformity
Appendix 1 Staining protocols Leica ST4040
1.1 Symbols used in this manual and their meaning
Warnings appear in a grey box and are
marked by a warning triangle.
Notes
i.e. important user information appear
in a grey box and are marked by an information symbol.
(5)
(Fig. 5)
Instrument type:
All information given in this instruction manual
applies only to the instrument type indicated on
the title page.
A name plate indicating the instrument serial
number is attached to the back of the instrument.
Required information for all enquiries:
For any enquiries please specify:
• Instrument type
• Serial number
Figures in brackets refer to item numbers in drawings or to the drawing itself.
6
Instruction Manual V1.4 – 06/2009
1. Important Information
General
This instruction manual includes important instructions and information related to the operating safety and maintenance of the instrument.
The instruction manual is an important part of
the product. It must be read carefully before
first using the instrument and must always be
kept with the instrument.
It will be necessary to add appropriate instructions to this instruction manual, if imposed by
existing national regulations or by laws on accident prevention and environmental protection in
the country of the operating authority.
Read this instruction manual carefully before
attempting to use or operate the instrument.
Please pay particular attention to the
safety instructions in chapter 2. Please
do read this information, even if you
are familiar with the operation and use
of Leica products.
1.2 Designated use
The Leica ST4040 is an automated linear stainer
for the preparation of histological and cytological routine stainings.
It is designed for use in pathology laboratories,
and only for performing the following tasks:
• Staining of thin sections of tissue specimens
or of cytological samples, attached to microscope slides
Any use of the instrument other than its designated use is considered improper.
Leica ST4040 – Linear Stainer
7
2. Safety
2.1 Safety instructions
This instrument has been built and tested in accordance with the following safety regulations
on electrical measuring, control, regulating and
laboratory devices.
In order to maintain this condition and to ensure
safe operation, the operator must observe the
instructions and warnings contained in this instruction manual.
For current information about applicable standards, please refer to the CE declaration of conformity on our Internet site:
www.histo-solutions.com
2.1.1 Transport and installation
• Do not operate the instrument in rooms
where risk of explosion exists!
• Do not expose the instrument to direct sunlight (windows)!
• Do not install the instrument above a heater!
• Install the instrument on an even laboratory
bench which must be absolutely level!
• Two people are needed to lift / carry the instrument!
• Before connecting the instrument to mains,
make sure the correct voltage setting,
matching the nominal voltage at the site of
installation, has been selected!
• Upon installing the drain hose make sure
there is a gradient from the drain outlet to
the waste pipe.
• To protect the user from hazardous solvent
fumes, make sure to operate the instrument
either with the activated carbon filter or the
exhaust air hose!
8
Instruction Manual V1.4 – 06/2009
2.1.2 Operating the instrument
2. Safety
• The instrument may only be operated by
skilled personnel. It may only be operated
according to its designated use and in accordance with the instructions given in this
manual!
• While working with reagents (filling / emp-tying the reagent stations, working on the instrument while the lid/s is/are open) appropriate protective gear (lab coat, gloves, safety goggles) must be worn!
• Make sure to operate the instrument ei-ther
with the activated carbon filter or with the
exhaust air hose (--> Chapter 3.6, ‘Installing
the accessories’). Even when the instrument
is operated according to its designated use,
hazardous solvent fumes develop, which are
damaging to the operator’s health and do
also pose a risk of fire!
• Risk of fire, when working with an open
flame (Bunsen burner) immediately next to
the instrument (solvent fumes)! - Therefore,
keep a safety distance of 1 meter!
• If not only the water tap but also the ball
valve has been closed (--> e.g. when setting
up the instrument), the tap water flow rate
has to be adjusted when starting a program
(see also chapters 3.6 and 6.8.3).
• The flow rate must not be too fast, to en-sure
that the specimens remain firmly at-tached
to the slide surface!
• If a staining program is to be interrupted for
an extended period of time, do not leave any
slide racks in the tap water sta-tions, in order
to prevent them from drying out!
• As soon as the alarm is triggered, immediately remove the completed slide rack from the
last station or from the unload container of
the optional unload station! Otherwise, immersion times for the remaining slide racks
will be prolonged!
• In case of emergency switch off mains and
unplug the power chord!
• If ‘Alarm off’ is selected (not recommended!), the instrument must be observed constantly, to make sure all slide racks are removed immediately when reaching the last
station!
Leica ST4040 – Linear Stainer
9
2. Safety
2.1.3 Cleaning and maintenance
• Only technical service engineers authorized
by Leica may access the internal components of the instrument for service and repair.
Exception: Changing the activated carbon filter is the only maintenance task to be carried out by the user.
• Prior to cleaning the instrument, always
switch off mains and unplug the power
chord!
• Dispose of used reagents according to the
laboratory regulations in force in your country!
• Spilled solvents (reagents) have to be wiped
away immediately! - In case of long-term exposure, the lid surfaces are only conditionally resistant to solvents!
• The painted surfaces and the control panel
are not resistant to xylene or acetone!
• When handling cleaning detergents, follow
the instructions of the manufacturer and
make sure all applicable laboratory regulations are complied with!
• When cleaning the instrument, no liquid may
enter in contact with any of the electrical
connections or get into the interior of the instrument!
• Wash the tap water and reagent stations in
the dishwasher at a temperature of max. +65
°C. - Use a standard detergent for laboratory
dishwashers.
At any rate avoid washing the stations at
higher temperatures (e. g. in industrial diswashers which run at a temperature of
+85 °C), as the stations may become deformed!
• For cleaning the instrument, do not use any
one of the following: alcohol, detergents
containing alcohol (window cleaner!), abrasive cleaning powders, solvents containing
xylene or acetone!
• To clean the lids, control panel and housing,
use mild household detergents; - see safety
instruction above for non-appropriate ingredients!
10
Instruction Manual V1.4 – 06/2009
3.1 Site requirements
The installation site has to meet the following
requirements:
• Stable laboratory bench, exactly level, at
least 1.60 m wide and 60 cm deep.
• Tap water supply at a distance of max. 2 m
and drain at a distance of max. 1.50 m from
the corresponding inlet and outlet at the
back of the instrument.
- Please bear in mind: The connections are
located at the extreme left on the back of the
instrument.
• Fume hood at a distance of max. 3.50 m from
the instrument, if the instrument is to be operated with the exhaust air hose (otherwise
the instrument must be operated with the
activated carbon filter)!
• Vibration-free floor.
3. Installation
Do not operate the instrument in rooms
where risk of explosion exists!
Do not expose the instrument to direct
sunlight (windows)!
Do not install the instrument above a
heater!
• Sufficient open space (70 cm) above the lab-
oratory bench, to ensure trouble-free opening of the lids.
• Stable ambient temperature of +10 °C to +35
°C.
• Relative air humidity of maximum 80%, non-
condensing.
• No other instruments which cause vibra-
tions installed nearby.
Leica ST4040 – Linear Stainer
11
3. Installation
3.2 Unpacking the instrument
The unpacking instructions for all Leica instruments are located in a transparent protective envelope on the outside of the instrument transportation crates.
3.2.1 Repacking the instrument
We recommend keeping the original crate and
packing material in store, in case the instrument needs to be repacked in the future.
The drawing below shows the design of the
original crate and packing material. The numbers indicate the sequence of disassembling
and reassembling the crate.
9
8
7
6
5
12
4
3
2
1
Instruction Manual V1.4 – 06/2009
3. Installation
3.3 Installing the instrument
• To lift, grip the instrument by the transport
handles.
Two people are needed to lift / carry
the instrument, since it weighs 73 kg
(see ‘Technical data’, chapter 5.1)!
• Install the instrument on the designated
bench.
• Unscrew the transport handles.
• Remove the plastic protective cover from the
instrument.
• Check all delivered parts against the packing
list to verify whether the delivery is complete
- see chapter 3.4 ‘Standard delivery ‘basic
instrument’ and general accessories’).
Leica ST4040 – Linear Stainer
• For all further installation steps, see chapters 3.5 ‘Electrical connection’ and 3.6 ‘Installing the accessories’.
13
3. Installation
3.4 Standard delivery ‘basic instrument’ and general accessories
3.4.1 Standard delivery
Basic instrument ST4040 - single load model
• Basic instrument with three-piece lid
• 27 Reagent stations, plastic
• 4 tap water stations, complete assy. with inlet valve
• 1 Drain hose for waste water, 2 m long
• 1 Tap water inlet hose, 2.50 m long,
complete with 3/4“ connection for water tap and replacement gasket
• 2 Lids for reagent stations
• 1 Cover for second row
• 1 Instruction manual ................................................................ 14 0474 80001
• 1 Tool set, including:
• 1 Single-ended open-jaw wrench, size 27
• 1 Single-ended open-jaw wrench, size 13
• 1 Screw driver, 5.5 x 200 mm
• 1 Screw driver, 3 x 50 mm
• 1 Water tap adapter for 1/2“ water taps
• 3 Disposable plastic films, solvent-resistant, for control panel
• 1 Set of power chords:
• Euro
•UK
• USA
14
Basic instrument ST4040 - double load model (for double row staining)
To use a single load model for double row staining, order the appropriate number (may vary - depends on individual staining applications) of the accessories listed below (see chapter 3.4.2 - ‘General
accessories’ for details):
• Reagent stations
• Tap water stations
• Lids for reagent stations
• Slide racks
• Slide rack carriers
Instruction Manual V 1.4 – 06/2009
3.4.2 General accessories
• Reagent stations, plastic
• Tap water stations, complete assy. with inlet valve
• Leica slide racks, metal
• Sakura slide racks, plastic
• Adapter for large slides
• Drain hose, 4 m long
• Tap water inlet hose, 2.50 m long,
• Lids for reagent stations
• Slide rack carriers for Leica slide racks
• Slide rack carriers for Medite/Hacker slide racks
• Slide rack carriers for Sakura slide racks
• Storage container for slide rack carriers, fits on instrument housing
• Activated carbon filter
• Exhaust air hose, 2 m long
• Exhaust air hose, 4 m long
• Disposable plastic films, solvent-resistant, for control panel (set of 10)
• Optional load station, left*
• Optional load station, right*
• Optional unload station, left*
• Optional unload station, right*
• Leica CV5000 adapter for slide racks:
3. Installation
complete with 3/4“ connection for water tap
• CV5000 slide rack adapter, removable:
- slides onto Leica slide racks for coverslipping in Leica CV5000.
- up to Leica CV5000 s/nos.
• CV5000 stationary slide rack adapter:
- for permanent installation in Leica CV5000 for using Leica
ST4040 slide racks in CV5000.
- starting from Leica CV5000 s/nos. > CV026096)
≤ CV026096
Leica ST4040 – Linear Stainer
To order accessories, please contact your Leica local sales organization for the latest literature / current order numbers on the full
range of Leica ST4040 accessories.
*) For accessories for optional load / unload station, please refer to
chapters 4.3.1 and/or 4.3.2 respectively!
15
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