Leica Sp9000 User Manual

Instruction Manual

Leica SP9000 V2.2 English – 08/2007
Always keep this manual near the instrument.

Read carefully prior to operating the instrument.

Leica SP9000

Automatic knife sharpener

IMPORTANT NOTE

The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and state­of-the-art technology as we understand it follow­ing thorough investigation in this field. We are un­der no obligation to update the present manual
periodically and on an ongoing basis according to
the latest technical developments, nor to provide
our customers with additional copies, updates etc.
of this manual.

For erroneous statements, drawings, techni­cal illustrations etc. contained in this manual
we exclude liability as far as permissible ac­cording to the national legal system applicable
in each individual case. In particular, no liabil­ity whatsoever is accepted for any financial loss
or consequential damage caused by or related
to compliance with statements or other infor­mation in this manual.

Statements, drawings, illustrations and other
information as regards contents or technical
details of the present manual are not to be con­sidered as warranted characteristics of our

products. These are determined only by the
contract provisions agreed between ourselves
and our customers.
Leica reserves the right to change technical
specifications as well as manufacturing pro­cesses without prior notice. Only in this way is
it possible to continuously improve the tech­nology and manufacturing techniques used in
our products.
This document is protected under copyright
laws. Any copyrights of this document are re­tained by Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – in­cluding any electronic systems and media –
requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.

For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.

© LeicaBiosystems Nussloch GmbH

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany

Telephone:[phone]
Facsimile: [phone]
eMail: [e-mail]
Internet : http://www.histo-solutions.com

Leica SP9000 – Automatic knife sharpener

Serial no.: ....................................................................

Year of manufacture: .................................................

Country of origin: ...Federal Republic of Germany

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Table of contents

1. Important Notes .............................................................................................................. 5

1.1 Symbols used in this manual and their meaning ............................................................... 5

1.2 Designated use / misuse .......................................................................................................... 6

1.3 Selection and qualification of personnel ............................................................................. 6

2. Safety................................................................................................................................. 7

2.1 General information on instrument design and safe handling ....................................... 7

2.2 Safety instructions for handling the instrument ................................................................. 7

3. Instruments properties ................................................................................................. 8

3.1 Technical Data ........................................................................................................................... 8

3.2 General Description .................................................................................................................. 8

3.3 Overview - SP9000 ..................................................................................................................... 9

4. Installation..................................................................................................................... 10

4.1 Check list .................................................................................................................................... 10

4.2 Installing the instrument......................................................................................................... 11

5. Coarse honing procedure ........................................................................................... 13

5.1 . Inspect for presence and size of nicks .............................................................................. 13

5.2. Put honing plate on holder .................................................................................................... 14

5.3. Apply coarse abrasive to the plate ..................................................................................... 14

5.4. Attach knife ............................................................................................................................... 15

5.5. Centre the knife using a ruler ................................................................................................ 15

5.6 Set coarse honing knob ......................................................................................................... 16

5.7 Set Timer at 30 minutes .......................................................................................................... 16

5.8 Push the blue coarse button on the control panel .......................................................... 16

5.9 Remove knife carefully ........................................................................................................... 16

5.10 Clean knife - inspect condition ............................................................................................. 17

5.11 Clean glass plate - continue Coarse Honing if necessary............................................. 17

6. Fine coarse procedure ............................................................................................... 18

7. Redressing hone glass plates .................................................................................. 21

8. Trouble shooting .......................................................................................................... 26

9. Warranty and service ................................................................................................. 27

10. EC Declaration of Conformity .................................................................................... 28

Peoples Republic of China......................................................................................... 29

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Instruction manual V 2.2 – 08/2007

1. Important Notes

The individual chapters of the
Leica SP9000 instruction manual:

Chapter 1 Important notes

• Table of contents

• Important information on this
manual

Chapter 2 Safety

• Make sure to read this
chapter before operating the
instrument!

Chapter 3 Instrument properties

• Technical Data

• Overview

Chapter 4 Installation

• Standard delivery

• Unpacking and installing the
instrument

Chapter 5 Coarse honing procedure

• Controls

• Working with the instrument

Chapter 6 Fine coarse procedure
Chapter 7 Redressing hone glass plates

1.1 Symbols used in this manual and their meaning

Warnings
appear in a grey box and are marked
by a warning triangle:

Useful notes,
i.e. important user information appear
in a grey box and are marked by an in­formation symbol:

(5)
(Fig.5)

Instrument type:

All information given in this instruction manual
applies only to the instrument type indicated on
the title page.

A name plate, indicating the instrument serial
number, is attached to the back of the instrument.

Figures in brackets refer to item num­bers in drawings or to the drawings
themselves.

Chapter 8 Trouble shooting

Chapter 9 Warranty and service

Leica SP9000 – Automatic knife sharpener

Required information for all inquiries:

For any inquiries please specify:

• Instrument type

• Serial number

5

1. Important Notes

General

This instruction manual includes important instructions and information related
to the operating safety and maintenance of the instrument.

The instruction manual is an important part of the product. It must be read
carefully before using the instrument for the first time and must always be
kept with the instrument.

If additional requirements, which exceed the scope of this manual, are im­posed by regulations and/or laws on accident prevention and environmental
protection in the country of operation, appropriate instructions for compli­ance with such requirements must be added to this manual.

Read this instruction manual carefully before attempting to work on or oper­ate the instrument.

1.2 Designated use / misuse

Please pay particular attention to chapter 2 (safety features,
safety instructions).
– Please read this information, even if you are already familiar
with the operation and use of other Leica products.

- The Leica Automatic Knife Sharpener is specifically designed to quickly and
accurately hone microtome knives to a precise and fine cutting edge. Addi­tionally, provision has been made to eliminate the tedious task of manually
redressing the honing plates. In total, the new sharpener will provide you
with greatly increased efficiency in honing, convenience in redressing plates,
and a considerable amount of valuable time saved.

- The instrument may only be operated according to the instructions con­tained in this manual.

- Any other use of the instrument is considered contrary to its designated use.

1.3 Selection and qualification of personnel

- The automatic knife sharpener SP9000 may only be operated by
trained laboratory personnel.

- Prior to starting work with the instrument, all laboratory personnel des­ignated to operate the instrument must carefully read the present instruc­tion manual and must be familiar with all technical features of the instru­ment.

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Instruction manual V 2.2 – 08/2007

2.1 General information on instrument design and safe handling

This instrument has been built and tested in accordance with the safety
regulations for electrical measuring, control, regulating and laboratory
devices.

In order to maintain this condition and ensure safe operation, the opera­tor must observe all the instructions and warnings contained in this in­struction manual.

For current information about applicable standards, please refer to the
CE declaration of conformity on our Internet site:

www. histo-solutions.com

2.2 Safety instructions for handling the instrument

2. Safety

Potential hazard

Caution: risk of injury when touch­ing the knives and blades as these
are extremely sharp.

Warning: Avoid touching live parts
under any circumstances!

2.2 Safety instructions for hand­ling the instrument

Correct behavior

Make sure to handle knives and blades very cau­tiously!
Never touch the cutting edge of knives and
blades!
Do not leave knives, blades and bladed knife hold­ers unprotected.

The instrument cover may only be removed by
qualified service personnel!
Before removing the cover, ensure that the instru­ment is unplugged.

Leica SP9000 – Automatic knife sharpener

7

3. Instruments properties

3.1 Technical Data

Power supply : Overall Dimensions :

Model 930 is 115 V / 60 Hz Width : 370 mm
Model 940 is 230 V / 50 Hz Height : 460 mm

3.2 General Description

Your technical skill is the key to the successful performance of any labora­tory equipment. While the Leica Microtome Knife Sharpener is efficient and
simple to operate, the results you get depend entirely on your knowledge of
how badly damaged the knife is and how to determine when your knife is to
be sharpened.
Consequently, careful microscopic examination of the edge and facets is
necessary before you start to resharpen, so you can decide on the amount
of coarse honing required. Also, when you complete the coarse and fine hon­ing procedure, it is recommended that the knife be examined microscopi­cally, to assure yourself that good results have been obtained.

Depth : 540 mm

The unit is designed to sharpen Leica Histostat knives up to 250
mm in length. Do not attempt to sharpen any Leica knife with the
Knife Sharpener that has been previously sharpened by another
method unless it has first been factory reconditioned by Leica.

See later section on reconditioning for information on restoring knives, elimi­nating large nicks, etc.

While under certain specific conditions competitive knives may be
sharpened on the Leica Knife Sharpener, optimum results cannot
be assured.

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Instruction manual V 2.1 – 04/2003

3.3 Overview - SP9000

Plastic cover

Glass Plate

Glass plate
thumbscreen

3. Instrument properties

Knife holder

Hone glass plate
holder

Timer

Plate positional
dial

Off Lap Fine Coarse

Leica SP 9000 – Automatic knife sharpener

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