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Leica SM2400
Sledge microtome
Instruction manual
Leica SM2400 - Sledge microtome
V1.1 English – 12/89
Always keep this manual near the instrument!
Read carefully prior to operatimg the instrument!
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NOTE
The information, numerical data, notes and value judgments contained in this manual represent the current
state of scientific knowledge and state-of-the-art
technology as we understand it following thorough investigation in this field.
We are under no obligation to update the present
manual according to the latest technical developments, nor to provide our customers with additional
copies, updates etc. of this manual.
For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability
as far as permissible according to the national legal
system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information
in this manual.
Statements, drawings, illustrations and other information as regards contents or technical details of the
present manual are not to be considered as warranted
characteristics of our products.
These are determined only by the contract provisions
agreed between ourselves and our customers.
Leica reserves the right to change technical specifications as well as manufacturing processes without prior
notice. Only in this way is it possible to continuously
improve the technology and manufacturing techniques
used in our products.
This document is protected under copyright laws. Any
copyrights of this document are retained by Leica Microsystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any
parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Microsystems Nussloch
GmbH.
For the instrument serial number and year of manufacture, please refer to the name plate at the back of the
instrument.
© Leica Microsystems Nussloch GmbH
Published by:
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Telephone: +49 (0)6224 143-0
Fax: +49 (0)62 24 143-200
eMail: histo_info@leica-microsystems.com
Internet: http://www.histo-solutions.com
Leica SM2400 - Sledge microtome
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Fig. 1 Leica SM2400 Sledge microtome
1 Coarse height adjustment lever
2 Hand guard
3 Coarse height adjustment clamp lever
4 Tilt angle adjustment screw
5 Knife clamp screw
6 Knife holder clamp screw
7 Knife holder with knife guard
8 Knife
9 Knife guard (closed)
10 Knife block
11 Ball joint
12 Ball joint clamp
13 Specimen holder clamp lever
14 Sledge clamp
15 Stop for automatic specimen feed
16 Sledge
17 Specimen holder manual height adjustment
18 Section thickness adjustment
19 Base
20 Guide rails
4 Instruction manual V1.1 English – 12/89
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Assembly
After removing the metal bands and the carton top,
use the supplied key to unscrew the four socket head
screws (2.2)* from below.
Lift the microtome off the wooden block (2.1) and place
it on a stable workbench.
After unscrewing the socket head screw (2.3), remove
the sledge clamping block (2.4).
Clean the guide rails (1.20) with petroleum spirit and
apply the supplied oil to be found in the accessories
box (see p. 8, Cleaning and Maintenance).
*(2.2), e.g., means figure 2, component 2
1 Wooden block (for transit)
2 Microtome-block screws
3 Socket head screws
4 Sledge clamping block
Slide the knife holder (1.7) into the knife block (1.10)
and clamp with the wing nut (1.6). After loosening the
clamp lever (1.12), rotate the specimen clamp (3.4) until
the clamp screw (3.1 or 5.1) faces the operator.
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4
Abb. 2
Leica SM2400 - Sledge microtome
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Operation
Move the sledge to the front stop and clamp with the
lever (1.14). Move the coarse height adjustment clamp
lever (1.3) to the right and lower the specimen holder
(3.4) as far as possible (lever 1.1).
Clamp the specimen in the holder (screw 3.1 or 3.5).
Set the knife tilt angle (scale on left of knife holder)
using screw (1.4) before inserting the knife and clamping with the two wing nuts (1.5).
Loosen the sledge clamp (1.14).
Slide the specimen under the knife. Loosen the univer-
sal joint clamp (3.7) and align the specimen with the
knife. Retighten the clamp.
Move the specimen using lever (1.1) until it is a few
millimeters from the knife (not touching). Clamp the
specimen holder in this position by turning the lever
(4.2) to the left.
Using the manual height adjustment control (4.1), and
with to and fro movements of the sledge, trim the
specimen until the surface is flat.
Set the section thickness (4.3). Move the sledge back
to the rear stop, and then forwards under the knife to
make the actual sections.
1 Specimen clamp screw
2 Specimen sledge
3 Specimen holder
4 Specimen x/y alignment screws
5 Universal joint
6 Specimen holder clamp
7 Universal joint clamp
1
5
4
6
2
1 Manual specimen holder height adjustment
3
7
(coarse)
2 Coarse height adjustment clamp lever
3 Section thickness control
2
1
3
Fig. 3 Kardangelenk-Klemme Fig. 4
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Accessories
The universal joint and the large specimen stage can
be easily interchanged after pressing the clamp lever
(3.6) down and removing the specimen holder. The
knife holder with integrated guard can be replaced
with the acute angle version (Fig. 7).
The safety caps must be placed over the
knife ends immediately it is inserted in the
holder.
If thick specimens are to be sectioned, the knife holder
can be raised by means of spacers (Fig.7). These are
fixed to the knife blocks (1.10) using socket head
screws.
The knife ends must be covered by the supplied caps.
Fig. 5 Large (90 x 130 mm) specimen stage
Cleaning and Maintenance
The sledge guide rails (1.20) must always be kept
clean. We recommend xylene or petroleum spirit.
Cleaning is accomplished most easily by moving the
sledge to and fro several times whilst cleaning both
sides of the rails. This ensures that dirt and tissue particles which have collected under the sledge are removed.
After cleaning, the guide rails must be well oiled using
the special oil No. 601 available from us:
50 ml Order No. 0336 21783
500 ml Order No. 0336 21818
Leica SM2400 - Sledge microtome
Fig. 7 Spacer on the left knife blockAbb. 6 Acute angle knife holder
The specimen holder guide rails (3.2) should be
cleaned as follows:
Move the specimen holder to its uppermost position
using lever (1.1), then clean the rails with xylene or
petroleum spirit. No. 410 grease should then be ap-
plied.
When not in use, the microtome should be protected
from dust etc. by means of the cover.
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8. Warranty and service
Warranty
Leica Microsystems Nussloch GmbH guarantees that the contractual product delivered
has been subjected to a comprehensive quality control procedure based on the Leica inhouse testing standards, and that the product is faultless and complies with all technical
specifications and/or agreed characteristics warranted.
The scope of the warranty is based on the content of the concluded agreement. The
warranty terms of your Leica sales organization or the organization from which you have
purchased the contractual product shall apply exclusively.
Technical service information
If you require technical service or replacement parts, please contact your Leica sales
representative or dealer who sold the product.
Please provide the following information:
• Model name and serial number of the instrument.
• Location of the instrument and name of the person to contact.
• Reason for the service call.
• Date of delivery.
Decommissioning and disposal
The instrument or parts of the instrument must be disposed of in compliance with the local laws.
8 Instruction manual V1.1 English – 12/89
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