Leica SM2000 R
Sliding Microtome
Instruction Manual
Leica SM2000R – V2.1 English – 03/2004
Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
1. Important information
Serial No. ...................................................................
Year of manufacture ................................................
Manufactured in: .. Federal Republic of Germany
The model, serial number and year of manufacture are
specified on the nameplate on the left of the instrument.
The information, numerical data, notes and value judgments contained in this manual represent the current
state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional
copies, updates etc. of this manual.
For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability as far as permissible according to the national
legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or
related to compliance with statements or other information in this manual.
Statements, drawings, illustrations and other information as regards contents or technical details of the
present manual are not to be considered as warranted
characteristics of our products. These are determined
only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical specifications as well as manufacturing processes without
prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products.
This document is protected under copyright laws. Any
copyrights of this document are retained by Leica
Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any
parts thereof) by means of print, photocopy, microfiche,
web cam or other methods – including any electronic
systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the name plate at the back of
the instrument.
© Leica Biosystems Nussloch GmbH
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17-19
D-69226 Nussloch
Germany
Telephone: 0 62 24/143-0
Telefax: 0 62 24/143-200
eMail: histo_info@leica-microsystems.com
Homepage: http://www.histo-solutions.com
Leica SM2000 R – Sliding Microtome
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2. Table of contents
1. Important information ........................................................................................................................ 3
2. Table of contents ................................................................................................................................ 4
3. Safety instructions for handling the instrument........................................................................... 5
3.1 Safety devices ....................................................................................................................................... 5
4. Technical data..................................................................................................................................... 7
5. General description............................................................................................................................ 8
5.1 Leica SM2000R - Overview ................................................................................................................................ 8
5.2 General description...........................................................................................................................................10
5.3 Standard delivery ................................................................................................................................ 10
6. Unpacking and Setup....................................................................................................................... 11
6.1 Unpacking ........................................................................................................................................................... 11
6.2 Site requirements ..............................................................................................................................................11
6.3 Setup ...................................................................................................................................................................12
6.4 Mounting the accessories................................................................................................................. 13
7. Operating the instrument ................................................................................................................ 14
7.1 Mounting and orienting the specimen ...........................................................................................................14
7.2 Clearance angle adjustment on the knife holder .........................................................................................14
7.3 Inserting the knife or disposable blade holder ............................................................................................. 14
7.4 Trimming ............................................................................................................................................................. 15
7.5 Sectioning ............................................................................................................................................ 15
8. Trouble shooting ............................................................................................................................... 16
9. Cleaning and maintenance ............................................................................................................. 17
9.1 Cleaning .............................................................................................................................................................. 17
9.2 Maintenance........................................................................................................................................ 17
10. Accessories ....................................................................................................................................... 18
10.1 Disposable blade holder ...................................................................................................................................18
10.2 Section waste tray ............................................................................................................................................18
10.3 Distance plate for knife holder ......................................................................................................... 18
11. Ordering information ....................................................................................................................... 19
12. Warranty and service ...................................................................................................................... 20
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Instruction manual V2.1 – 03/2004
3. Safety instructions for handling the instrument
3.1 Safety devices
Fig. 5.1
14
The safety devices on the instrument and accessory equipment must not be removed or
modified!
The instrument incorporates the following safety devices: Knife guard (14) on the knife holder, knife sledge
locking knob (23) and magnetic knife sledge immobilizer (24).
Knife guard on the knife holder
Prior to manipulating the knife or specimen,
or changing the knife or specimen, and during breaks, always cover the cutting edge
with the knife guard (14)!
Knife sledge locking knob
Prior to changing the specimen or knife and
before transporting the instrument, lock the
knife sledge with the locking screw (23).
Fig. 5.2
24
23
Magnetic knife sledge immobilizer
Caution! The knife sledge movement is extremely smooth. It may occur that the sledge
moves during section removal.
To prevent the sledge from moving accidentally, pull it to the front limit of the slideway,
where it is held in position by the magnetic
knife sledge immobilizer (24), before removing the section.
Leica SM2000 R – Sliding Microtome
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3. Safety instructions for handling the instrument
Transport and setup
• The instrument must be transported in an
upright position!
• Before transporting the instrument, the
knife sledge must be locked with the locking screw (23, Fig. 4.2)!
• Do not carry the instrument by holding it at
movable parts!
• The safety devices on the instrument and
accessory equipment must not be removed
or modified!
Operating the instrument
• Take care when handling microtome
knives and disposable blades!
The cutting edge is extremely sharp and
may cause severe injury!
• Never leave knives and knife holders with
a mounted knife or blade lying around! Always put the knives back into the knife
case when not in use!
Cleaning
• Before cleaning, remove the knife or disposable blade!
• Do not use solvents that contain acetone
or xylene!
• Ensure that no liquids enter the interior of
the instrument when cleaning!
• When using detergents, please comply
with the safety precautions of the manufacturer!
Maintenance
• Only qualified and authorized service personnel may access the internal components of the instrument for service and repair!
• Do not place a knife on a table with the
cutting edge facing upwards!
• Never try to catch a falling knife!
• Caution! The knife sledge movement is extremely smooth. It may occur that the
sledge moves during section removal.
To prevent the sledge from moving accidentally, pull it to the front limit of the
slideway, where it is held in position by
the magnetic knife sledge immobilizer (24),
before removing the section.
• Always clamp the specimen first clamping before the knife!
• Prior to manipulating the knife or specimen, or changing the specimen or knife,
and during breaks, always cover the cutting edge with knife guard and lock the
knife sledge with the locking knob!
• Always wear protective glasses when
sectioning brittle specimens!
Risk of splintering!
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Instruction manual V2.1 – 03/2004
Type SM 2000 R
Sliding microtome
Operating
temperature range: +10°C to +40°C
Section thickness
setting: 1-60 μm
0-2 μm in 0.5 μm increments
2-10 μm in 1 μm increments
10-20 μm in 2 μm increments
20-60 μm in 5 μm increments
Automatic
specimen advance: 0-30 μm
Overall feed: 40 mm
Dimensions and weight
Width: 300 mm
Depth: 440 mm
Height: 330 mm
4. Technical data
Weight
(incl. knife holder): 25.5 kg
Leica SM2000 R – Sliding Microtome
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