Leica POWER MOSAIC, LAS POWER MOSAIC User Manual

LAS Power Mosaic

Get the Big Picture

LAS Power Mosaic is designed to bring a whole
new dimension to microscopy, providing you
with a unique understanding of the relationship
between microscopic features and the overall
structure of the specimen.

LAS Power Mosaic provides the ultimate in
high-resolution specimen visualisation. Scan
the entire specimen or select a specifi c region
of interest and it’s scanned at high speed. The
captured images are immediately combined to
form a seamless mosaic image.

Once the scan is complete you can relocate
effortlessly to areas of interest with a single
mouse click and can view the live microscope
image that corresponds to a chosen location.
Additionally you can zoom and pan around the
entire digitised mosaic image using easy and
intuitive “browsing” tools.

The acquired images, including the entire
mosaic can be saved for subsequent review,
discussion with colleagues or publication.

LAS Power Mosaic is ideal for both routine and
research applications and provides modes of
scanning to suit all forms of optical microscopy.
It’s specifi cally developed to be both versatile
and easy to use. This powerful LAS option
provides the microscopist with a high
performance mosaic imaging solution while
retaining conventional use of the microscope.

Z-Stack Mosaic

In addition to the standard scanning and review
capabilities, the Power Mosaic Plus module
further extends the capability using “Z-Stack”
acquisition to provide 3-D mosaic imaging and
visualisation, ideally suited to specimens with a
wide focal range.

Key benefits include:

�

Scanning and acquisition at camera frame
rates to ensure high speed and rapid
throughput

�

Tile edge merging provides highest possible
image quality

�

No restrictions on objective or imaging
method

�

Fast and accurate relocation with easy and
intuitive review tools

�

Intelligent memory management supports
mosaic sizes limited only by available disk
space

�

Fully automated focus control and tracking

�

User control of image size to ensure
suitability for publication and archiving.

�

One click calibration for easy camera
alignment

�

Optional advanced functions, including
powerful 3-D Z-Stack mosaic acquisition for
powerful focus visualisation

Features at a Glance

Power Mosaic Scanning

�

Uses triggered image capture for fast

continuous scan and acquire

�

Standard scan available using step and

acquire for low light applications

�

Image streaming for mosaic sizes only

limited by disk space

�

Additional scans can be easily added to

extend an initial scan

Scan Patterns

�

Rectangle, Circular, Annular, Cross (+ and x),

or Random

�

Overlap of tiles allows joins to be merged

smoothly

�

Camera rotation is automatically corrected

�

Create a scan pattern interactively or by

entering the exact details

Microscope Automation

�

An Oasis XY stage and Z focus control drive

board is used

�

A software joystick or Leica smart move can

be used for stage and focus movement

�

Fully compatible with Leica Microsystems

LAS confi gured microscopes handling focus,
turret, condenser and lamp controls as
available

Leica DFC Camera

�

Exposure, saturation, gain and gamma

control from LAS controls

�

Triggered acquisition from progressive scan

and DFC FX cameras for fastest scans

�

Automatic and manual white balance

�

Color or monochrome acquisition (8 or 16-bit)

�

Shading correction for smooth mosaic

results