Leica DM1000 LED User Manual
Leica DM1000
Leica DM1000 LED
Operating Manual · Bedienungsanleitung · Mode d’emploi
Published June 2010 by/
Herausgegeben Juni 2010 von/
Publiée en june 2010 par :
Leica Microsystems CMS GmbH
Ernst-Leitz-Strasse 17-37
D-35578 Wetzlar (Germany)
Responsible for contents/
Verantwortlich für den Inhalt/
Responsable du contenu rédactionnel :
Peter Schmitt, Manuela Jacobsen
(Clinical Imaging, Product Management)
Holger Grasse
(Safety Offi cer according to MPG §30/
Sicherheitsbeauftragter nach MPG §30/
Responsable de la sécurité conformément à la loi
relative aux dispositifs médicaux, §30)
In case of questions, please contact the hotline/
Bei Fragen wenden Sie sich bitte an die Hotline/
Pour toute question, contacter notre service
d’assistance téléphonique :
Phone/Tel./Tél. +49 (0) 64 41-29 4253
Fax +49 (0) 64 41-29 2255
E-Mail/e-mail/courriel MQM-Hotline@leica-
microsystems.com
Leica DM1000
Leica DM1000 LED
Operating Manual
Copyrights
Copyrights
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica
Microsystems CMS GmbH.
The instructions contained in the following
documentation reflect state-of-the-art technology.
We have compiled the texts and illustrations as
accurately as possible. Nevertheless, no liability
of any kind may be assumed for the accuracy of
this manual’s contents. Still, we are always
grateful for comments and suggestions
regarding potential mistakes within this
documentation.
The information in this manual is subject to modification at any time and without notification.
4
Contents
Contents
1. Important Notes about this Manual ...... 6
2. Intended Purpose of the Microscope ... 7
3. Safety Notes ............................................... 8
3.1 General Safety Notes ............................... 8
3.2 Electrical Safety ........................................ 8
3.3 Disposal ....................................................... 9
4. Overview of the Instrument .................... 10
5. Unpacking the Microscope .................... 14
6. Assembling the Microscope .................. 16
6.1 Stage ............................................................ 16
6.2 Condenser ................................................... 18
6.3 Tube and Eyepieces .................................. 19
6.4 Objectives ................................................... 19
6.5 Light Source - Transmitted Light Axis ... 20
6.6 Components for
Fluorescence Applications...................... 21
6.6.1 Fluorescence Illuminator .............. 21
6.6.2 106z Lamp Housing ......................... 21
6.7 Analyzer and Polarizer* .......................... 24
6.8 Lambda Plate Compensator .................... 24
6.9 Optional Accessories ............................... 25
6.10 Insertion of the batteries ......................... 27
6.11 Connection to the Power Supply............ 27
7. Startup ......................................................... 28
7.1 Switching on the Microscope................. 28
7.2 Köhler Illumination .................................... 28
7.3 Checking Phase Contrast Rings ............. 29
7.4 Adjusting the Light Sources .................... 31
8.3 Focusing ...................................................... 36
8.4 Tubes....................................................... 37
8.5 Eyepieces.................................................... 38
8.6 Objectives ................................................... 39
8.7 Light Sources ............................................. 40
8.8 Aperture Diaphragm ................................. 40
8.9 Field Diaphragm ......................................... 41
9. Contrast Methods ...................................... 42
9.1 Transmitted Light ....................................... 42
9.1.1 Brightfield......................................... 43
9.1.2 Phase Contrast................................ 44
9.1.3 Darkfield ........................................... 44
9.1.4 Oblique Illumination ....................... 45
9.1.5 Polarization ...................................... 45
9.2 Fluorescence.............................................. 46
10. Measurements with the Microscope ... 47
10.1 Linear Measurements .............................. 47
10.2 Thickness Measurements ....................... 48
10.3 Differentiation of Gout / Pseudo Gout ... 49
11. Trouble Shooting ....................................... 51
12. Care of the Microscope ........................... 54
12.1 Dust Cover .................................................. 54
12.2 Cleaning....................................................... 54
12.3 Handling Acids and Bases ...................... 55
12.4 Changing Fuses.......................................... 55
13. Essential Wear and Spare Parts ............ 56
14. Retrofitting Components .......................... 57
14.1 Equipping the Condenser Disk................ 57
8. Operation .................................................... 35
8.1 Switching on............................................... 35
8.2 Stages and Object Displacement........... 35
15. Index ............................................................ 59
16. EC Declaration of Conformity ................. 60
5
1. Important Notes about this Manual
1. Important Notes about this Manual
Caution!
This operating manual is an essential component of the microscope, and must be read
carefully before the microscope is
assembled, put into operation or used.
Text symbols, pictograms and their meanings:
(1.2)
→
p. 20
!
This operating manual contains important instructions and information for the operational
safety and maintenance of the microscope and
accessories. Therefore, it must be kept and
taken care of.
Numbers in parentheses, such as "(1.2)", correspond to illustrations (in the example, figure 1,
item 2).
Numbers with pointer arrows (for example
→ p. 20), point to a certain page of this manual.
Caution!
Special safety instructions within this
manual are indicated with the triangle
symbol shown here, and have a gray
background.
Caution! The microscope and accessories can
be damaged when operated incorrectly.
Notes on the disposal of the device,
accessories and consumable materials.
Explanatory note
*
6
Item not contained in all configurations
2. Intended Purpose of the Microscope
2. Intended Purpose of the Microscope
The Leica DM1000/DM1000 LED microscope, to
which this user manual belongs, is designed for
biological routine and research applications.
This includes the examination of samples taken
from the human body with a view to provide
information on physiological or pathological
states or congenital abnormalities, or to
determine the safety and compatibility with potential recipients, or to monitor therapeutic
measures.
The above-named microscope complies with
the Council Directive 98/79/EEC concerning in
vitro diagnostics. (It also conforms to the
Council Directives 2006/95/EC concerning
electrical apparatus and 2004/108/EC
concerning electromagnetic compatibility for
use in an industrial environment.)
The manufacturer assumes no liability for
damage caused by, or any risks arising from
using the microscope for other purposes
than those for which they are intended or
not using them within the specifications of
Leica Microsystems CMS GmbH.
In such cases the conformity declaration
shall cease to be valid.
These (IVD) devices are not intended for use
in the patient environment defined by DIN
VDE 0100-710. Neither are they intended for
combining with medical devices according
to EN 60601-1. If a microscope is electrically
connected to a medical device according to
EN 60601-1, the requirements defined in
EN 60601-1-1 shall apply.
Not suitable for examining potentially
infectious specimens.
Caution!
Caution!
7
3. Safety Notes
3. Safety Notes
3.1 General Safety Notes
This safety class 1 (DM1000) or class 2
(DM1000 LED) device is constructed and tested
in accordance with
EN 61010-2-101:2002 (DM1000/DM1000 LED),
EN 61010-1:2001 (DM1000/DM1000 LED),
IEC 61010-1:2001 (DM1000/DM1000 LED),
IEC 60825-1:2007 (DM1000 LED)
EN 60825-1 + A1 + A2:2003 (DM1000 LED),
LED Class I (DM1000 LED)
safety regulations for electrical measuring, control, and laboratory devices.
Caution!
In order to maintain this condition and to ensure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.
Caution!
The devices and accessories described in
this operating manual have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar, Germany must be consulted
whenever the device is altered, modified or
used in conjunction with non-Leica components that are outside of the scope of this
manual.
3.2 Electrical Safety
General Specifications
Microscope
For indoor use only.
Supply voltage:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Over voltage category:
Pollution degree:
Specifications of the external power supply
ELPAC POWER SYSTEMS power supply,
Model: FW1812
Input: 100-240 V AC
0,5 A
47-63 Hz
Output: 12 V DC
1,5 A max.
18 W max.
Caution!
90-250 V AC, 50-60 Hz
(DM1000)
12 V DC, 1.5 A
(DM1000 LED)
40 W (DM1000)
18 W (DM1000 LED)
F 3.15 A 250 V
(DM1000)
Integrated in external
power supply unit,
not replaceable
(DM1000 LED)
15-35°C
max. 80% to 30°C
II
2
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims and product liability!
8
Use the original power supply only. Other
power supplies must not be used.
3. Safety Notes
If the original power supply fails or is
damaged, it must be replaced. Repair is not
permitted.
Original power supplies are available from
your Leica branch office or Leica dealer.
Caution!
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire inside or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!
Caution!
DM1000 only: Never use any fuses as
replacements other than those of the types
and the current ratings listed here. Using
patched fuses or bridging the fuse holder is
not permitted. The use of incorrect fuses
may result in a fire hazard.
Caution!
Protect the microscope from excessive temperature fluctuations. Such fluctuations can
lead to the accumulation of condensation,
which can damage the electrical and optical
components.
Ambient temperature: 15-35°C.
Caution!
DM1000 only: Before exchanging the fuses
or lamps, be absolutely certain to switch off
the main power switch and remove the power cable.
3.3 Disposal
To dispose of the product at the end of its service life, please contact Leica Service or Sales.
Please observe national laws and regulations,
such as those implementing and enforcing the
WEEE EU directive.
Note:
Caution!
The microscope's electrical accessory components are not protected against water.
Water can cause electric shock.
Like other electronic devices, the microscope and its accessory components must
not be disposed of as regular household
waste.
9
4. Overview of the Instrument
4. Overview of the Instrument
Specification
Contrast Methods
Transmitted Light Axis
Incident Light Axis
(optional)
Tube
Leica DM1000/DM1000 LED
• Transmitted light: brightfield, darkfield,
phase contrast, polarization
• Incident light: fluorescence
Leica DM1000: Integrated halogen illumination
Leica DM1000 LED: Integrated LED illumination
manual adjustment of
• Light intensity
• Aperture diaphragm
• Field diaphragm (only with Köhler kit)
Incident light fluorescence illuminator for eyepieces with
field number up to 20 with
• Interchangeable slide with mount for 3 filter systems
• Adjusting lens for lamp
• Light trap for the suppression of extraneous light
• BG38 blue filter and shutter, switchable
optionally with
• Fixed or variable viewing angle
• Up to 3 switching positions
• one or two camera ports
• Ergotube with height-adjustable eye level and camera port
Magnification Changer
(optional)
Objective Turret
X/Y Stage
10
• Manual
• Magnification steps: 1x; 1.5x; 2x
• Manual
• 5-fold for objectives with M25 thread
• With condenser holder
• Coaxial pinion, optional telescopable
• Controls mountable left or right
4. Overview of the Instrument
Specification
Condenser
Focusing
Leica DM1000/DM1000 LED
optionally with
• CL/PH 0.90/1.25 OIL condenser with color coding
• CLP/PH 0.85 condenser for polarization
• Achr.apl. A 0.9 (P) condenser with swivelable condenser head
• UCL 0.90/1.25 OIL universal condenser UCLP 0.85 for
polarization with 5-position light ring disk)
• UCL/P pol. universal condenser with interchangeable
condenser head and condenser disk with 6 positions
• Focus wheel for coarse and fine focusing
• Height adjustment
11
4. Overview of the Instrument
6
5
Fig. 1 Left side of the Leica DM1000 stand
1 Coarse and fine focusing
2 Condenser height adjustment
3 Brightness control
4 Field diaphragm
5 Aperture diaphragm
6 Condenser
12
4
12 3
4. Overview of the Instrument
1
2
3
4
5
Fig. 2 Right side of the Leica DM1000 stand
1 Eyepiece
2 Eyepiece tube
3 Tube
4 Objective turret with objectives
5 Specimen stage with specimen holder
6 Integrated illumination
7 On/Off switch
8 Condenser height adjustment
9 Coarse and fine focusing
10 Coaxial pinion for x/y stage movement
678910
13
5. Unpacking the Microscope
5. Unpacking the Microscope
First, carefully remove all components from the
transportation and packaging materials.
Note:
If at all possible, avoid touching the lens surfaces of the objectives. If fingerprints do appear
on the glass surfaces, remove them with a soft
leather or linen cloth. Even small traces of finger
perspiration can damage the surfaces in a short
time. See the chapter "Care of the Microscope"
p. 54, for additional instructions.
→
Caution!
Do not yet connect the microscope and peripherals to the power supply at this point!
Installation Location
Work with the microscope should be performed
in a dust-free room, which is free of vapors (oil,
chemicals etc.) and of extreme humidity. At the
workplace, large temperature fluctuations,
direct sunlight and vibrations should be avoided.
These conditions can distort measurements and
micrographic images.
Allowable ambient conditions
Temperature 15-35°C
Relative humidity maximum 80% up to 30°C
Microscopes in warm and warm-damp climatic
zones require special care in order to prevent
the build up of fungus.
See the chapter "Care of the Microscope" →
for additional instructions.
Caution!
Electrical components must be placed at least
10 cm away from the wall and away from
flammable substances.
p. 54,
14
Transport
For shipping or transporting the microscope
and its accessory components, the original
packaging should be used.
As a precaution to prevent damage from vibrations, the following components should be disassembled and packaged separately:
• Unscrew the objectives
• Remove the condenser
• Remove the coaxial pinion
• Remove the lamp housings
• Disassemble the burner of 106z lamp housing
• Remove all moving or loose parts
5. Unpacking the Microscope
15
6. Assembly
6. Assembling the Microscope
The microscope components are logically assembled in this order:
• Stage
• Condenser
• Fluorescence illuminator*
• Intermediate systems*
• Tube
• Eyepieces
• Objectives
• Lamp housings with light sources*
• Polarization equipment*
Only one commonly used screwdriver is
necessary for assembly, which is included in the
delivery package.
The tool can be stored on a magnetic retainer
on the underside of the stage at the right.
When using intermediate systems and optical
accessories, the sequence may vary.
In this case, read Chapter
"6.9 Optional Accessories" → p. 25.
6.1 Stage
Caution:
!
Before completing the stage, make sure no objectives are installed!
Remove the srew located under the stage in the
front.
Specimen Holder
Coaxial Pinion
Note:
The coaxial pinion can be mounted on the leftor right-hand side.
• First, place the flat fine focus wheel on the
side to which you intend to mount the coaxial
pinion; the wheel is held in place magnetically
(4.1); ensure that the button snaps into place.
Attach the other focus knob on the opposite side
• Loosen the lock screw (5.1) at the front lefthand side of the stage
• Slide the stage as far back as possible
• Attach the coaxial pinion with the srew (6.1)
• Return the stage to the starting position and
retighten the lock screw; after installation of
the stage control, move object guide all the
way to the left side of the instrument; keep
turning when guide has reached the end of
travel until a click noise is heard
Fig. 3 Specimen stage with specimen holder
1 Lock screws for specimen holder
1
• Place the specimen holder on the stage and
fasten it with the two screws (3.1)
16
6. Assembly
Adjusting the Focus Stop
The focus stop is preadjusted by the factory to
prevent collision with objectives. The focus stop
should be set approx. 0.3 mm higher as the focal
plane to enable focussing of the samples of different thickness.
If a readjustment is necessary, adjust as
following:
• Lower the stage by rotating 1/2 turn of the
coarse adjustment knob
• Loosen the focus stop screw on left hand side
of microscope
• Move stage to desired focal plane (preferably
approx. 0.3 mm higher)
• Tighten focus stop screw
Stage Lock*
The stage lock is mounted in the same hole as
the stage drive (in case of ErgoStages, it can be
mounted on the opposite position in addition to
stage drive).
The mounting of stage lock is similar to
mounting of the stage drive:
• Loosen the locking screw (5.1) on the left side
underside of stage and move the stage all the
way back
• Loosen the screw for coaxial pinion (6.1),
remove the coaxial pinion, and attach the
stage lock with this screw
• Loosen the stage lock screw and pull the
stage forward to the desired position
• Retighten the locking screw (5.1) underside of
stage
• Press the pinion of the stage lock against the
rack and retighten the stage lock screw
Fig. 5 Underside of stage
1 Lock screw
1
Fig. 4 Focus wheel
1 Magnetic retainer for fine focus wheel
Fig. 6 Coaxial pinion installation
1 Mounting screw for coaxial pinion
1
1
17
6. Assembly
6.2 Condenser
• If present, screw the condenser head into the
condenser
• Using the condenser height adjuster (9.3), turn
the condenser holder (fig. 8) completely
downward
• Unscrew the clamping screw for the condenser (9.2) far enough so that the condenser
can be inserted from the front
• From the front, insert the condenser into the
condenser holder as far as it will go; on the
underside of the condenser, there is an orientation pin (7.1), which must be located in the
guiding notch (8.1)
• Pull the condenser's clamping screw (9.2) so
that the condenser is locked in place
Fig. 7 Underside of condenser (example CL/PH)
1 Orientation pin
2 Auxiliary condenser lens LS
Note:
The condenser must be centered before using
the microscope.
Köhler illumination p. 28.
→
Fig. 8 Condenser holder
1 Guiding notch
1
Fig. 9 Condenser holder
1 Condenser centering bolts
2 Clamping screw for condenser
3 Condenser height adjuster
18
1
1
3
1
3
2
1
2
6. Assembly
6.3 Tube and Eyepieces
Note:
For fluorescence applications, install the
fluorescence illuminator* first → p. 21.
If available, the analyzer* (10.1) must be
inserted into the stand. This requires that the
guide key engages in the guide pin (10.2).
To mount the analyzer, the analyzer mount TL*
20 mm or 60 mm can also be placed between
stand and tube.
An intermediate tube pole* with a switchable
analyzer (on/off) and Bertrand lens is also
available as an option.
The tube is mounted to the stand either directly or
with the use of intermediate modules*.
• Loosen the clamping screw (11.1) on the
stand
• Insert the tube in the circular receptacle
(dovetail ring)
• Retighten the clamping screw (11.1)
• The eyepieces are inserted into the eyepiece
tubes on the tube
6.4 Objectives
Always only use Leica objectives of tube length ¥
(infinity)! The standard thread is M25. The
objectives should be arranged so that the
magnification increases when the objective
nosepiece is rotated counter-clockwise.
!
Attention:
Lower the specimen stage as far as possible
before assembling the objectives. Close vacant
threads in the nosepiece with dust protection
caps!
Fig. 10 Analyzer mounting
1 Analyzer
2 Orientation pin and guiding notch
3 Clamping screw
Fig. 11 Fastening the tube
1 Clamping screw
1
19
6. Assembly
6.5 Light Source for the Transmitted Light Axis
Caution!
Note:
The Leica DM1000 LED is equipped with
integrated LED illumination. The service life of
the LED is about 100.000 hours. If, despite this, it
should be necessary to change the LED, this
task must be carried out by Technical Service
only.
The following instructions in this chapter refer to
the Leica DM1000 with tungsten halogen lamp.
Caution!
Be sure that the microscope and lamp
housing are disconnected from the power
supply. Unplug the power plug and the power supply during assembly.
Light sources pose a potential irradiation
risk (glare, UV-radiation, IR-radiation).
Therefore, lamps have to be operated in
closed housings.
Replacing the Lamp of the Integrated
Illumination
The transmitted light illumination with a lowvoltage tungsten halogen lamp (fig. 12) is
integrated in the base of the microscope and is
accessible from the right-hand side.
• Remove the insert (12.2)
Caution!
The lamp may still be hot!
• Remove the lamp
Caution!
Fig. 12 Transmitted-light illumination
in Leica DM1000 microscope base
1 Tungsten halogen lamp
2 Insert
12
20
Do not remove the new lamp's dust cover
until you have installed the lamp. Avoid
fingerprints on the lamp.
• Insert the new lamp with the dust cover
straight into the socket until it stops; be sure
that the lamp is inserted straight
• Remove the lamp's dust cover
• Replace the insert (12.2)
6.6 Components for Fluorescence Applications*
6.6.1 Fluorescence illuminator*
6. Assembly
Caution!
The fluorescence illuminator is mounted before
the tube. It is fastened in place with the side
clamping screw (13.1).
6.6.2 106z Lamp Housing*
Caution!
Light sources pose a potential irradiation
risk (glare, UV-radiation, IR-radiation).
Therefore, lamps have to be operated in
closed housings.
During assembly, always unplug the power
supply unit of the 106z lamp housing from its
socket.
During assembly work on xenon burners, always wear the supplied protective gloves
and face protection (fig. 14) (risk of explosion).
Never touch the glass parts of the burner
with bare hands.
Never look directly into the beam path
(blinding hazard).
Make sure to follow the instructions and
safety notes of the lamp supplier.
Before changing lamps allow at least 30 mins
for cooling down!
Inserting the Gas Discharge Lamps* (Hg and
Xe) into the 106z Lamp Housing
Hg and Xe lamps are powered by separate
supply units.
Read the separate instruction manual provided
with these supply units.
Fig. 13 Assembly of fluorescence illuminator
1 Clamping screw
1
Fig. 14
Protective gloves and mask
The lamp housing 106z is used with various gas
discharge lamps.
21
6. Assembly
The following gas discharge lamps may be used
and require different supply units and lamp
mounts (fig. 16):
Type Typical Bulb Life
50 W high-pressure mercury burner (alternating current) 100 hours
100 W high-pressure mercury burner (direct current) 200 hours
100 W high-pressure mercury burner, type 103 W/2 (direct current) 300 hours
75 W high-pressure xenon burner (direct current) 400 hours
+) Please regard the data sheets for the burners.
• To open the 106z lamp housing, unscrew the
fastening screws (15.8) on the cover
• Remove the transport anchorage (red plastic
rod in place of the burner) in the lamp mount;
to do so, remove the lower clamp (16.1); pull
up the cooling element (16.3) and turn it to the
side; detach the lower clamp system (16.2)
and remove the transport anchorage
• Install the burner in reverse order
Caution!
Fig. 15 106z lamp housing (on the side, open)
1 Cover (raised)
2 Collector
3 Gas discharge lamp in mount
4 Reflector (mirror)
5, 6, 7 Adjusting screw for x-y reflector
8 Fastening screw for lamp mount
9 Socket for contact plug
+)
Hg 50 Burner:
After installation, the labeling must be
upright.
If a glass melt nipple is present (16a.4), position
it by turning the burner so that the nipple does
not come in the way of the beam path later, but
instead is positioned
sideways.
Xe 75 Burner:
Remove the burner's dust cover (16b.5) after
you have installed the burner.
22
1
2
4
5
3
6
7
898
6. Assembly
• Insert the lamp mount, with the burner installed, into the lamp housing and tighten it
with the screws (15.8)
• Close the lamp housing and retighten the
screws
• Place the lamp housing in the incident light
lamp housing receptacle (17.1) and fasten it
with the clamping screw on the side
• Connect the lamp housing to the external
power supply
Fig. 16 a-c Lamp mounts for gas discharge lamps
1 Upper clamping system
2 Lower clamping system
3 Cooling element
4 Nipple of the mercury 50 burner,
5 Dust cover of the mercury 75 burner
Fig. 17 Mounting the 106z lamp housing
1 Lamp housing receptacle
1
Hg 50
1
4
a
3
2
Xe 75
b
Hg 100
3
1
1
c
3
5
2
2
23
6. Assembly
6.7 Analyzer and Polarizer*
Analyzer
If the analyzer was inserted into the tube mount
before the tube assembly: (→ p. 19), no additional assembly step is required.
If an intermediate tube pole* or analyzer mount
TL* is used:
• Remove the plug cap on the left side
• Insert the analyzer into the receptacle until it
latches in place
Polarizer
• Raise the condenser to its upper stop position
• Remove the DLF filter magazine from the base
if present
• Press the polarizer holder in place (Fig. 18)
• Push the polarizer with the labeled side
upward into the lower opening
Alternative:
• Attach the polarizer holder to the underside of
the condenser holder with the left clamp
screw (19.1); remove the flip-out blue filter if
required
• Push the polarizer with the labeled side
upward into the lower opening
6.8 Lambda Plate Compensator*
• Raise the condenser to its upper stop position
• Remove the DLF filter magazine from the base
if present
• Attach the lambda plate compensator to the
base
Fig. 18 Filter holder*
with two positions
24
Fig. 19 Assembly of polarizer holder*
1 Clamping screw
1
6. Assembly
6.9 Optional Accessories
Camera*
A camera can be connected via an adapter.
• Attach the adapter to the top port of the tube
and fasten it tightly with the side clamping
screw
• Screw on the camera
Note:
The size of the camera chip and the mounting
system (B-mount, C-mount, etc.) must be
considered when choosing an adapter (see
table).
Calculation of the magnification on the monitor
The magnification M
on the monitor can be
TV
calculated with the following formula or
measured with a stage micrometer and a cm
scale:
= Objective magnification x
M
TV
factor of magnification changer* x
TV adapter magnification* x
monitor diameter
chip diameter of camera
Recorded picture diagonal in mm for
1 inch 2/3 inch 1/2 inch 1/3 inch
camera camera camera camera
Without Zoom Magnification, only for 1-Chip-Cameras:
C-mount adapter 1 x HC 16 11 8 6
C-mount adapter 0.70 x HC - 15.7 11.4 7.8
C-mount adapter 0.55 x HC - - 14.5 10.9
C-mount adapter 0.35 x HC - - - 17.1
With Zoom Magnification (Vario TV Adapter) for 1-3 Chip-Cameras:
C-mount, 0.32-1.6 x HC - - 19
+)
-5 18-3.8
B-mount (ENG), 0.5-2.4 x HC (1/2-inch) - - 16-3.3 -
+)
from zoom factor 0.42 x only!
Without Zoom Magnification, for 1-3 Chip-Cameras:
C-mount adapter 1 x - - 16 12
B-mount adapter 1 x - - 16 12
B-mount adapter 1.25 x - 17.5 - F-mount adapter 1 x - - 16 12
F-mount adapter 1.25 x - 17.5 - Plus (essential requirement): TV optics 0.5 x HC
25
6. Assembly
Ergomodule*
For raising the eye level of the tube opening, the
30 mm or 60 mm ergomodule may be used.
It is fastened in place with the side clamping
screw.
Installation of the tube on the 60 mm ergomodule: The tube has to be rotated 90° degree
(eyetubes going to the right) and rotated back
into the viewing position and tightened with the
screw.
Ergolift*
A base for the stand featuring adjuster wheels
for the base’s height and angle is available to
ensure an optimal working position.
Magnification Changer*
Optionally, a magnification changer (fig. 20) can
be used, which is manually operated. On the
knurled ring, the following magnification factors
can be set:
Viewing Attachments*
Viewing attachments featuring illuminated
pointers are available for groups of 2, 3, 4, 5 and
10 viewers respectively (other configurations on
request).
The support (21.3) must be aligned precisely.
The fade-in arrow can be moved in x and y
direction.
Fig. 21 Viewing attachment
1 Movement of light pointer in x and y direction
2 Brightness control
3 Adjustment of arm support
The external power supply (illuminated arrow) is not
illustrated.
1
2
3
1x; 1.5x; 2x
Fig. 20 Magnification
changer
26
Tracing Device*
The tracing device L3/20 (fig. 22) allows an
optical overlay of large objects (next to the
microscope) on the microscope image. This
makes it easy to draw specimens by tracing
their outlines or superimposing scales.
Fig. 22 Tracing device
1 Shutter
1
6. Assembly
6.10 Inserting the batteries (DM1000 LED only)*
The microscope can be powered by batteries if
you prefer. The batteries are automatically
charged when the microscope is connected to
the mains supply.
The microscope can be used for about 6-8 hours
in battery-operated mode.
• The battery compartment is accessed from
underneath the stand (23a.1); remove the lid
of the compartment
• Insert the batteries (order no. p. 56) as shown
on the bottom of the compartment (fig. 23b)
and close the lid
Fig. 23a Bottom of stand (DM1000 LED)
1 Lid of the battery compartment
6.11 Connection to the Power Supply
• After completing the assembly work, connect
the stand to the power supply using the power
cable supplied (fig. 23c)
• When using the lamp housing or the
external power supply unit, connect them to
the power supply, too
Fig. 23c Back of the stand (DM1000)/Type label
1 Power supply connection
1
1
Fig. 23b Bottom of stand (DM1000 LED)
1 Battery compartment open
1
Fig. 23d Back of the stand (DM1000 LED)/Type label
1 External power supply connection
1
27
7. Start-up
7. Start-up
7.1 Switching on the Microscope
• Switch on the microscope with the on/off
switch (24.1)
Caution!
After turning on the gas discharge lamp*,
the burner must be immediately adjusted.
Therefore, do not turn on the power supply*
unit yet. First, work in transmitted light in order to familiarize yourself with the microscope’s controls.
Fig. 24
1 On/Off switch
2 Focus wheel
3 Stage positioning
7.2 Köhler Illumination*
The condenser is also pre-adjusted in the
factory.
However, it may be necessary to re-adjust the
condenser in some cases. Therefore, check the
condenser centering.
The following procedure is provided for the
transmitted light brightfield illumination.
• If present click the condenser disk* into the
BF position
• If present pull the light ring slide* out of the
condenser
• Select an objective with moderate
magnification (10x-20x);
for condensers with movable condenser
heads:
Swing in the condenser top
(The condenser top is swung out for objective
magnifications < 10x.)
28
• Insert the specimen into the stage’s specimen
holder
• Focus on the specimen using the focus wheel
(24.2)
• Set the light intensity using the brightness
control (25.2)
• Close the field diaphragm (25.3) until the edge
of the diaphragm appears in the specimen
plane (26a)
21 3
7. Start-up
• Using the condenser height adjuster (25.1),
adjust the condenser until the edge of the
field diaphragm appears in sharp relief (26b)
• If the image does not appear in the middle of
the field of view (26c), the condenser must be
moved into the middle of the field of view with
the help of the two centering bolts (25.4); the
tool required for this purpose is magnetically
attached to the underside of the stage
• Open the field diaphragm just enough for it to
disappear from the field of view (26d)
Note:
The condenser height adjustment depends on
the thickness of the specimen. It may be
adjusted for different specimens.
Fig. 25
1 Condenser height adjuster
2 Brightness control
3 Field diaphragm
4 Condenser centering
7.3 Checking Phase Contrast Rings
If your microscope is equipped for the use of
phase contrast, the light rings that fit the objectives are built into the condenser disk*.
The light rings are already centered in the
factory. However, the centering should be
rechecked.
Note:
A light ring slide which is inserted into the side
of the condenser is used for condensers without
condenser disks. Centering is not required in
this case.
Note:
When swivelling in a suitable objective for
phase contrast, the corresponding light ring
must be chosen.
The objective engraving (e.g. PH 1) indicates the
corresponding light ring (e.g. 1).
Fig. 26 Köhler illumination
a Field diaphragm not focused, not centered
b Field diaphragm focused, but not centered
c Field diaphragm focused and centered,
diameter is too small, however
d Field diameter (light) = Field diameter (view)
(Köhler illumination)
12 3
4
a
b
cd
29
7. Start-up
• In the place of an eyepiece, insert the focusing telescope (fig. 27) into the observation
tube
• Swivel in the phase contrast objective with
the lowest magnification
• Focus on the specimen with the focus wheel
• Focus the ring structure (29.a) by slightly loosening the clamping ring (27.2) and moving the
eye lens (27.1)
• Retighten the clamping ring
• Select the corresponding ring diaphragm
(light ring) in the condenser.
• If the light ring and the phase ring are not
shown as arranged in fig. 29.c, the light ring
must be centered
• Insert the centering screws into the openings
provided at the rear of the condenser (28.1)
• Turn the centering screws until the dark ring
(phase ring in the objective) is congruent with
the slightly narrower bright ring (light ring in
condenser) (29 c)
• Repeat the process for all other light rings and
objectives
• Optionally remove the centering keys after the
centering procedure
Fig. 28 Light ring centering (i.e.: condenser UCL/P)
1 Centering keys
11
Fig. 27 Focusing telescope
1 Adjustable eye lens
2 Clamping ring for fixing the focus position
1
2
30
Fig. 29 Phase contrast centering procedure
PH=phase contrast ring, LR=light ring
a Condenser in bright field (BF) position
b Condenser in phase contrast (PH) position
Light ring (LR) not centered
c Light ring and phase ring centered
ab c
PH LR
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