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Genova Nano Spectrophotometer
Micro Volume Accessory
Accessory Fitting and Operating Manual
737 555 REV A/03-12
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Safety
Please read this information carefully prior to installing or using this equipment.
1. The unit described in this manual is designed be operated only by trained personnel. Any
adjustments, maintenance and repair must be carried out as defined in this manual, by a person qualified
to be aware of the hazards involved.
2. It is essential that both operating and service personnel employ a safe system of work, in addition to
the detailed instructions specified in this manual.
3. Other than for those items defined in the maintenance procedures herein there are no user serviceable
items in this instrument. Removal of covers and attempted adjustment or service by unqualified personnel
will invalidate the warranty and may incur additional charges for repair.
4. References should always be made to the Health and Safety data supplied with any chemicals used.
Generally accepted laboratory procedures for safe handling of chemicals should be employed.
5. If it is suspected that safety protection has been impaired in any way, the unit must be made inoperative
and secured against any intended operation. The fault condition should immediately be reported to the
appropriate servicing authority.
Merci de lire attentivement ces informations avant d’installer ou d’utiliser cet appareil.
1. L’appareil décrit dans ce manuel est conçu pour être utilisé uniquement par des personnes formées.
Tout réglage, maintenance ou réparation doit être effectué comme décrit dans ce manuel, par une
personne qualifiée consciente des risques encourus.
2. Il est essentiel que les personnes utilisant et intervenant sur cet appareil respectent les règles de
sécurité de travail, en plus des instructions détaillées précisées dans ce manuel.
3. En-dehors des éléments décrits dans les procédures de maintenance ci-incluses, cet appareil ne
contient aucun élément réparable par l’utilisateur. L’enlèvement des capots et les tentatives de réglage
ou de réparation par des personnes non qualifiées invalide toute garantie et entraîne un risque de frais
de réparation supplémentaires.
4. Toujours se référer aux fiches techniques de santé et de sécurité accompagnant tout produit chimique
utilisé. Respecter les procédures de laboratoire généralement acceptées pour la manipulation en toute
sécurité des produits chimiques.
5. Si l’utilisateur suspecte qu’un problème quelconque puisse mettre en cause la sécurité, l’appareil doit
être rendu inopérant en empêchant son utilisation. Communiquer la défaillance constatée au service de
maintenance compétent.
Bitte lesen Sie diese Hinweise vor Installation oder Gebrauch dieser Ausrüstung sorgfältig durch.
1. Das in diesem Handbuch beschriebene Gerät darf nur von geschultem Personal bedient werden.
Alle Anpassungen, Wartungsarbeiten und Reparaturen müssen entsprechend der Vorgaben in diesem
Handbuch und von einer kompetenten Person, die mit den damit verbundenen Gefahren vertraut ist,
durchgeführt werden.
2. Es ist wichtig, dass sowohl das Bedienungs- als auch das Service-Personal zusätzlich zu den detaillierten
Anweisungen in diesem Handbuch ein sicheres Arbeitssystem einsetzen.
3. Mit Ausnahme der Teile, deren Wartungsverfahren in diesem Handbuch beschrieben sind, enthält
dieses Gerät keine weiteren Teile, die vom Benutzer gewartet werden können. Das Entfernen von
Abdeckungen und Versuche von hierfür unqualifiziertem Personal, Anpassungen oder Wartungsarbeiten
durchzuführen, haben zur Folge, dass die Garantie verfällt und können zusätzliche Reparaturkosten
auslösen.
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4. Es ist jederzeit auf die sicherheitsrelevanten Daten sämtlicher verwendeter Chemikalien Bezug zu
nehmen. Allgemein anerkannte Labormethoden zum sicheren Umgang mit Chemikalien sollten
eingesetzt werden.
5. Besteht der Verdacht, dass die Sicherheitsvorrichtungen in irgendeiner Weise beschädigt wurden,
muss das Gerät außer Betrieb genommen und gegen weiteren Gebrauch gesichert werden. Die Störung
sollte der zuständigen Serviceeinrichtung unverzüglich gemeldet werden.
Leggere attentamente queste istruzioni prima di installare o utilizzare il dispositivo.
1. L’unità descritta nel presente manuale è stata realizzata per essere utilizzata solo da personale che ha
ricevuto l’apposita formazione. Qualsiasi operazione di regolazione, manutenzione e riparazione deve
essere effettuata sulla base di quanto indicato nel presente manuale da personale qualificato consapevole
dei rischi connessi.
2. È fondamentale che il personale operativo e il personale addetto alla manutenzione utilizzino un
sistema di lavoro sicuro, oltre a seguire le istruzioni specificate nel presente manuale.
3. Oltre a quelli indicati nelle procedure di manutenzione, all’interno di questo dispositivo non sono
presenti altri elementi sui quali è possibile effettuare interventi. La rimozione delle protezioni e qualsiasi
tentativo di regolazione o di manutenzione posto in essere da personale non qualificato invaliderà la
garanzia. In questi casi, sarà necessario pagare un importo per le riparazioni effettuate.
4. È sempre necessario fare riferimento ai dati sulla salute e sulla sicurezza forniti con le sostanze chimiche
utilizzate. Adottare le procedure di laboratorio generalmente accettate per la gestione delle sostanze
chimiche.
5. Nel caso in cui si sospetti che la salute possa essere pregiudicata in qualsiasi modo, disattivare l’unità
per renderla inutilizzabile. Qualsiasi condizione di errore deve essere immediatamente segnalata al
responsabile per la manutenzione.
Lea esta información atentamente antes de instalar o utilizar este equipo.
1. La unidad descrita en este manual está diseñada para que solamente la utilice personal con formación.
Cualquier operación de ajuste, mantenimiento y reparación debe llevarse a cabo del modo indicado en
este manual y debe realizarla una persona cualificada que sea consciente de los peligros que implica.
2. Es fundamental que tanto los operarios como el personal de servicio utilicen un sistema de trabajo
seguro, así como las instrucciones detalladas que se especifican en este manual.
3. Cualquier elemento que no se encuentre entre los definidos en los procedimientos de mantenimiento
aquí descritos no podrá utilizarse en este instrumento. La extracción de las tapas y los intentos de ajuste
o reparación por parte de personal no cualificado invalidarán la garantía y pueden incurrir en cargos
adicionales por reparación.
4. Siempre deberían consultarse los datos sobre Salud y Seguridad que se suministran con cualquier
producto químico que se utilice. Es necesario llevar a cabo los procedimientos de laboratorio de aceptación
generalizada para la manipulación segura de productos químicos.
5. Si existe la sospecha de que las medidas protectoras de seguridad han quedado dañadas en cualquier
modo, la unidad debe inutilizarse y protegerse contra toda operación que se intente llevar a cabo.
El estado de fallo debe comunicarse inmediatamente a la autoridad de servicio de mantenimiento y
reparación pertinente.
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Contents
Page
SECTION 1 - INTRODUCTION 3
1.1 MICRO VOLUME ACCESSORY DESCRIPTION 3
1.2 MICRO VOLUME ACCESSORY SPECIFICATION 3
1.3 UNPACKING 4
SECTION 2 – ACCESSORY LAYOUT AND INSTALLATION 5
2.1 ACCESSORY LAYOUT 5
2.2 ACCESSORY REMOVAL 5
2.3 ACCESSORY INSTALLATION 7
2.4 INITIALISATION 7
SECTION 3 – ACCESSORY SETTINGS 8
3.1 ACCESSING THE MICRO VOLUME ACCESSORY SETTINGS 8
3.2 PATH LENGTH SELECTION 9
3.2.1 Known Path Length Measurements 9
3.2.2 Unknown Path Length Measurements 9
SECTION 4 – PERFORMING MICRO VOLUME MEASUREMENTS 10
4.1 PIPETTING SAMPLES ONTO THE MICRO VOLUME READ HEAD 10
4.2 SAMPLE RECOVERY OR REMOVAL 11
SECTION 5 – CALIBRATION OF THE MICRO VOLUME ACCESSORY 12
5.1 CALIBRATION SOLUTIONS (035 092) 12
5.2 ACCESSORY CALIBRATION PROCEDURE 12
5.2.1 Micro Volume Accessory Settings 12
5.2.2 Calibration Standard - Data Entry 12
5.2.3 Accessory Calibration and Verification 13
SECTION 6 – CLEANING AND DECONTAMINATION OF THE MICRO VOLUME ACCESSORY 16
6.1 ACCESSORY CLEANING 16
6.2 ACCESSORY DECONTAMINATION 16
6.3 READ HEAD RECONDITIONING 16
SECTION 7 – ACCESSORIES 16
7.1 ACCESSORIES 16
SECTION 8 – MAINTENANCE AND SERVICE 17
8.1 ROUTINE MAINTENANCE 17
8.2 SERVICE 17
SECTION 9 – TROUBLESHOOTING 18
9.1 ACCESSORY ERROR CODES 18
9.1.1 Calibration Procedure Error Codes 18
9.1.2 Verification Check Error Codes 18
9.2 TROUBLESHOOTING GUIDE 19
9.3 TECHNICAL SUPPORT 19
SECTION 10 – GLOSSARY OF ICONS 20
SECTION 11 – CHEMICAL COMPATIBILITY 21
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SECTION 1 - INTRODUCTION
1.1 MICRO VOLUME ACCESSORY DESCRIPTION
The Genova Nano is a UV/visible spectrophotometer dedicated to life science analysis. This
spectrophotometer incorporates a micro volume sample measurement accessory that allows sample
volumes as low as 0.5μl to be analysed. The Genova Nano spectrophotometer is pre-programmed with
methods to determine DNA concentrations and purity ratios using wavelengths recorded at 260, 280
and 230nm, with an optional correction at 320nm and the Bradford, Lowry, Biuret, BCA and Direct UV
methods for protein analysis. When the micro volume accessory is removed, the Genova Nano reverts to
a standard Genova Plus spectrophotometer with measurement modes for photometrics, concentration,
multi-wavelength, spectrum scanning, quantitation and kinetics.
This life science spectrophotometer uses icon driven software and has an improved navigation system
for easy and intuitive usability.
1.2 MICRO VOLUME ACCESSORY SPECIFICATION
Genova Nano
Wavelength
Range 198 to 1000nm
Resolution 1nm
Accuracy ± 2nm
Repeatability ± 0.5nm
Spectral bandwidth 5nm
Photometrics
Absorbance range -0.300 to 2.500A
(10mm path length equivalent = -15.0 to 125.0A)
Accuracy ±2%@ 1A
Absorbance Precision Between 0 and 1A = <0.005, 1 to 2A = 2%, above 2A = 4%
dsDNA Detection Limit (0.5mm) 2.0 ng/μl
Maximum
Concentration (0.20mm) 6000 ng/μl
Stray light <0.5% at 340nm and 220nm
Other
Weight 1.5kg
Path lengths 0.2mm & 0.5mm
Measurement time <6.5s
Sample size 0.5 to 5.0μl
Operating Temperature 10 to 40°C
Operating Humidity 0 to 80% non-condensing
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1.3 UNPACKING
The Genova Nano is delivered with the micro volume accessory pre-installed and securely packaged in
the spectrophotometers sample chamber. The protective packaging must be removed before the
instrument is first initialised.
Micro volume
protective
packaging
(Remove)
Fig 1.3 – Micro Volume Accessory Unpacking
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SECTION 2 – ACCESSORY LAYOUT AND INSTALLATION
2.1 ACCESSORY LAYOUT
Linkage Connection
Upper Read Head
Path Length
Drive Motor
Lower Read Head
Fig 2.1 – Micro Volume Accessory Layout
2.2 ACCESSORY REMOVAL
The micro volume accessory will be pre-installed when purchased as part of the Genova Nano
spectrophotometer. The accessory is optically matched to the spectrophotometer in which it is originally
installed. Users are therefore not able to transfer the micro volume accessory to other Genova Plus/Nano
spectrophotometers without the assistance of trained service personnel.
The micro volume accessory should not be removed or re-installed un-necessarily as this may
affect the accuracy of the accessories optical alignment.
The following steps must be followed to remove and re-install the micro volume accessory into the
Genova Nano spectrophotometer.
Read Head Cover
Side Covers
Remove the front panel from the sample chamber
by loosening screws 1 and 2 until the front panel
can be lifted out in the forward direction.
1 2
Remove front panel
▼
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Side
Covers
Remove the side covers by lifting them out
Screws 3 & 4
Remove the outer screws 3 and 4.
Screws 5 & 6
Linkage connection
Read Head Cover
Ensure that the inner screws 5 and 6 are not
adjusted/removed.
Disconnect the linkage connection from the bracket
found on the underside of the spectrophotometer lid
using the supplied Allen key and remove the read head
cover.
View with linkage disconected and read head cover
removed.
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Finally disconnect the power and communication cable
from the underside of the accessory PCB.
Rotate the
accessory to the
right to remove.
Carefully lift the micro volume accessory from the spectrophotometer sample chamber by rotating the accessory towards the right hand side so that the highlighted
detector optic is not knocked during the removal of the
accessory.
Detector optic
Finally disconnect the power and communication
cable from the underside of the
accessory PCB.
2.3 ACCESSORY INSTALLATION
The micro volume accessory is installed by reversing the procedure described in Section 2.2.
2.4 INITIALISATION
Connect the power supply unit to the power inlet socket on the rear panel of the instrument and
connect to the mains socket. Turn the power on at the mains and switch the instrument on using the
power switch on the rear of the instrument.
The instrument will initially check for firmware updates and then perform several power-on tests before
displaying the main menu:
Fig 2.2 – Genova Nano Main Menu
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SECTION 3 – ACCESSORY SETTINGS
Micro Volume Accessory
icon
A
260
Calibration
Standard Value
Tick icon
Micro Volume Accessory
icon
A
330
Calibration
Standard Value
Calibration Standard
Path Length Value
3.1 ACCESSING THE MICRO VOLUME ACCESSORY SETTINGS
When installed, the micro volume accessory icon is displayed in the bottom right hand corner of the
screen in each measurement mode.
The micro volume accessory settings are accessed by pressing the key below the micro volume accessory
icon.
The micro volume accessory settings allow the user to select the required path length for a measurement
and calibrate the accessory using a standard solution with known absorbance values at 260 and
330nm.
Micro Volume Accessory icon
0.2mm Path
Length Setting
0.5mm Path
Length Setting
Auto Path
Length Setting
Path Length
Calibration
Current Calibration
Factors
Fig 3.1 – Micro Volume Accessory Settings Menu
Accessory
Alignment Mode
Tick Icon
Calibration
A
260
Stanadard Value
Calibration
A
330
Stanadard Value
Calibration
Standard Path
Length Value
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3.2 PATH LENGTH SELECTION
3.2 PATH LENGTH SELECTION
3.2.1 Known Path Length Measurements
If the required measurement path length is known it
can be selected by pressing the button adjacent to
the 0.2mm or 0.5mm path length setting icons. The
selected setting is indicated by an icon with a black
background. Once the required path length setting
has been selected press the button adjacent to the
tick icon to confirm.
3.2 PATH LENGTH SELECTION
3.2.1 Known Path Length Measurements
If the required measurement path length is known it
can be selected by pressing the button adjacent to
the 0.2mm or 0.5mm path length setting icons. The
selected setting is indicated by an icon with a black
background. Once the required path length setting
has been selected press the button adjacent to the
tick icon to confirm.
3.2.2 Unknown Path Length Measurements
If the required measurement path length is not
known the auto path length setting can be selected
by pressing the button adjacent to the auto path
length setting icon. Once the auto path length setting
has been selected press the button adjacent to the
tick icon to confirm.
3.2.1 Known Path Length Measurements
3.2.2 Unknown Path Length Measurements
If the required measurement path length is known
it can be selected by pressing the button adjacent
to the 0.2mm or 0.5mm path length setting icons.
The selected setting is indicated by an icon with a
black background. Once the required path length
setting has been selected press the button adjacent
to the tick icon to confirm.
If the required measurement path length is not
known the auto path length setting can be
selected by pressing the button adjacent to the
auto path length setting icon. Once the auto path
length setting has been selected press the button
adjacent to the tick icon to confirm.
The auto path length measurement setting will firstly measure a sample using the 0.5mm path length
setting. If the measured photometric value is within range, the value will be displayed on screen and
no more measurements are taken. If however the measured value is over-range, the sample will be remeasured using the 0.2mm path length setting.
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SECTION 4 – PERFORMING MICRO VOLUME MEASUREMENTS
For detailed descriptions of the measurement modes that are available on the Genova Nano
spectrophotometer please refer to the supplied Genova Plus operating manual.
4.1 PIPETTING SAMPLES ONTO THE MICRO VOLUME READ HEAD
The Genova Nano spectrophotometer is designed to measure sample volumes ranging from 0.5μl to
5.0μl.
Jenway recommends that users should, if possible, use at least 2µl of sample for their
measurements.
1. With the read head mechanism open, pipette the
liquid to be analysed onto the centre of the lower
read head.
Pipetting a sample onto the read head
Read head in rest position
2. Close the lid of the spectrophotometer. This will
lower the read head assembly down onto the path
length drive motor. The upper read head will now
be in the rest position, 2mm above the lower read
head.
3. When a measurement is initiated, the path length
drive motor lowers the upper read head to the
specified measurement distance, the photometric
measurement is taken and the upper read head is
then returned to the rest position.
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Read head in measurement position
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4.2 SAMPLE RECOVERY OR REMOVAL
Once a measurement is complete, the sample solution can be recovered from the lower read head with
a suitable pipette or removed from the read heads by cleaning with a suitable lint free cloth.
1. Open the lid of the spectrophotometer. The read
head mechanism will open to allow access to the
upper and lower read heads.
2. The sample can be recovered by carefully drawing
the liquid that is retained on the lower read head
into a clean pipette.
3. Both read heads should then be wiped with a lint
free cloth.
4. More rigorous cleaning may be required after the measurement of high concentration samples or
samples that pose a contamination risk. See section 6 for further details.
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SECTION 5 – CALIBRATION OF THE MICRO VOLUME
SECTION 5 – CALIBRATION OF THE MICRO VOLUME ACCESSORY
Jenway recommends that the micro volume accessory is calibrated every 6 months. A set of
calibration solutions (Part code 035 092) are supplied with the Genova Nano spectrophotometer.
5.1 CALIBRATION SOLUTIONS (035 092)
The supplied calibration solution set consists of
two vials.
1. Matrix Blank (White)
2. 10x ref - Calibration Standard (Blue)
A calibration certificate is supplied that details the
certified absorbance values of the calibration
standard and the path length at which these values
were determined.
5.2 ACCESSORY CALIBRATION PROCEDURE
5.2.1 Micro Volume Accessory Settings
The micro volume accessory settings can be
accessed by pressing the key below the Micro
Volume Accessory icon in any of the Genova
Nano’s operating modes.
SECTION 5 – CALIBRATION OF THE MICRO VOLUME ACCESSORY
Jenway recommends that the micro volume accessory is calibrated every 6 months. A set of
calibration solutions (Part code 035 092) are supplied with the Genova Nano spectrophotometer.
5.1 CALIBRATION SOLUTIONS (035 092)
The supplied calibration solution set consists of
two vials.
1. Matrix Blank (White)
2. 10x ref - Calibration Standard (Blue)
A calibration certificate is supplied that details the
certified absorbance values of the calibration
standard and the path length at which these values
were determined.
5.2 ACCESSORY CALIBRATION PROCEDURE
5.2.1 Micro Volume Accessory Settings
The micro volume accessory settings can be
accessed by pressing the key below the Micro
Volume Accessory icon in any of the Genova
Nano’s operating modes.
5.2.2 Calibration Standard - Data Entry
1. Enter the A260 and
A330 DNA standard
solution values from the supplied calibration
certificate into the
accessory settings by
pressing the key adjacent to the Calibration
Standard Value icon.
ACCESSORY
Jenway recommends that the micro volume accessory is calibrated every 6 months. A set of calibration
solutions (Part code 035 092) are supplied with the Genova Nano spectrophotometer.
5.1 CALIBRATION SOLUTIONS (035 092)
The supplied calibration solution set consists of two
vials.
1. Matrix Blank (White)
White
5.2 ACCESSORY CALIBRATION PROCEDURE
5.2.1 Micro Volume Accessory Settings
label
1
2
Blue
label
2. 10x ref - Calibration Standard (Blue)
A calibration certificate is supplied that details the
certified absorbance values of the calibration standard
and the path length at which these values were
determined.
The micro volume accessory settings can be accessed
by pressing the key below the Micro Volume Accessory
icon in any of the Genova Nano’s operating modes.
Micro Volume Accessory icon
5.2.2 Calibration Standard - Data Entry
1. Enter the A260 and A330 DNA standard solution
values from the supplied calibration certificate
into the accessory settings by pressing the key
adjacent to the Calibration Standard Value icon.
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2. Select the digit to be changed using the keys
at the bottom of the screen. Use the keys
adjacent to the arrow
icons to increase or
decrease the number. Press the key adjacent
to the tick icon to save any changes.
2. Select the digit to be changed using the keys
at the bottom of the screen. Use the keys
adjacent to the arrow
icons to increase or
decrease the number. Press the key adjacent
to the tick icon to save any changes.
3. Enter the stated path length (1.0mm for the
supplied calibration standard solution set) for
the DNA standard solution values, in mm, into
the
accessory settings by pressing the key
adjacent to the Calibration Standard Path
Length Value icon.
2. Select the digit to be changed using the keys
at the bottom of the screen. Use the keys
adjacent to the arrow
icons to increase or
decrease the number. Press the key adjacent
to the tick icon to save any changes.
3. Enter the stated path length (1.0mm for the
supplied calibration standard solution set) for
the DNA standard solution values, in mm, into
the
accessory settings by pressing the key
adjacent to the Calibration Standard Path
Length Value icon.
4. Select the digit to be changed using the keys at the bottom of the screen. Use the keys
adjacent to the arrow icons to increase or decrease the number. Press the key adjacent to the
tick icon to save any changes.
5.2.3 Accessory Calibration and Verification
If an error message is displayed during the accessory calibration and verification procedure,
refer to Section 9 for further details.
1. The accessory calibration sequence is initiated
by pressing the key
adjacent to the Path
Length Calibration icon.
2. Select the digit to be changed using the keys
at the bottom of the screen. Use the keys
adjacent to the arrow
icons to increase or
decrease the number. Press the key adjacent
to the tick icon to save any changes.
3. Enter the stated path length (1.0mm for the
supplied calibration standard solution set) for
the DNA standard solution values, in mm, into
the
accessory settings by pressing the key
adjacent to the Calibration Standard Path
Length Value icon.
4. Select the digit to be changed using the keys at the bottom of the screen. Use the keys
adjacent to the arrow icons to increase or decrease the number. Press the key adjacent to the
tick icon to save any changes.
5.2.3 Accessory Calibration and Verification
If an error message is displayed during the accessory calibration and verification procedure,
refer to Section 9 for further details.
1. The accessory calibration sequence is initiated
by pressing the key
adjacent to the Path
Length Calibration icon.
2. The Air Measurement icon will be displayed.
Use a lint free cloth to clean the upper and
lower read heads, close the instrument lid and
press the key adjacent to the tick icon to
continue. Pressing the key
adjacent to the
cross icon will return the instrument to the
micro volume accessory settings menu screen.
2. Select the digit to be changed using the keys at
the bottom of the screen. Use the keys adjacent
to the arrow icons to increase or decrease the
number.
Press the key adjacent to the tick icon to save any
changes.
3. Enter the stated path length (1.0mm for the
supplied calibration standard solution set) for the
DNA standard solution values, in mm, into the
accessory settings by pressing the key adjacent
to the Calibration Standard Path Length Value
icon.
4. Select the digit to be changed using the keys at the bottom of the screen. Use the keys adjacent to
the arrow icons to increase or decrease the number. Press the key adjacent to the tick icon to save
any changes.
5.2.3 Accessory Calibration and Verification
If an error message is displayed during the accessory calibration and verification procedure,
refer to Section 9 for further details.
1. The accessory calibration sequence is initiated
by pressing the key adjacent to the Path Length
Calibration icon.
2. The Air Measurement icon will be displayed. Use
a lint free cloth to clean the upper and lower read
heads, close the instrument lid and press the key
adjacent to the tick icon to continue. Pressing
the key adjacent to the cross icon will return
the instrument to the micro volume accessory
settings menu screen.
The unit takes five dark and light readings at
260 and 330nm, at both the 0.5 and 0.2mm
pathlengths.
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The unit takes five dark and light readings at
260 and 330nm, at both the 0.5 and 0.2mm
pathlengths.
3. The Blank Solution Measurement icon will be
displayed. Open the instrument lid and pipette
a 2.0μl aliquot of the matrix blank solution onto
the lower read head. Close the instrument lid
and press the key adjacent to the tick icon to
initiate the blank measurement.
The unit takes five dark and light readings at
260 and 330nm, at both the 0.5 and 0.2mm
pathlengths.
3. The Blank Solution Measurement icon will be
displayed. Open the instrument lid and pipette
a 2.0μl aliquot of the matrix blank solution onto
the lower read head. Close the instrument lid
and press the key adjacent to the tick icon to
initiate the blank measurement.
4. The unit takes five readings at 260 and 330nm,
at both the 0.5 and 0.2mm pathlengths.
5. If the measured values are within the required tolerances, the Passes Test icon will be
displayed. Press the button adjacent to the Tick icon to continue or the button adjacent to the
Cross icon to cancel the calibration process.
6. The Calibration Solution Measurement icon will
be displayed. Open the instrument lid and use
a lint free cloth to clean the read heads.
7. Pipette a 2.0μl aliquot of the calibration solution
onto the lower read head. Close the instrument
lid and press the key adjacent to the tick icon to
The unit takes five dark and light readings at
260 and 330nm, at both the 0.5 and 0.2mm
pathlengths.
3. The Blank Solution Measurement icon will be
displayed. Open the instrument lid and pipette
a 2.0μl aliquot of the matrix blank solution onto
the lower read head. Close the instrument lid
and press the key adjacent to the tick icon to
initiate the blank measurement.
4. The unit takes five readings at 260 and 330nm,
at both the 0.5 and 0.2mm pathlengths.
5. If the measured values are within the required tolerances, the Passes Test icon will be
displayed. Press the button adjacent to the Tick icon to continue or the button adjacent to the
Cross icon to cancel the calibration process.
6. The Calibration Solution Measurement icon will
be displayed. Open the instrument lid and use
a lint free cloth to clean the read heads.
7. Pipette a 2.0μl aliquot of the calibration solution
onto the lower read head. Close the instrument
lid and press the key adjacent to the tick icon to
initiate the calibration solution measurement.
8. The unit takes five readings at 260 and 330nm,
at both the 0.5 and 0.2mm pathlengths.
9. If the measured values are within the required tolerances, the Passes Test icon will be
displayed. Press the button adjacent to the Tick icon to continue or the button adjacent to the
Cross icon to cancel the calibration process.
10. The Calibration Solution Measurement icon will
be displayed for a second time
. Open the
instrument lid and use a lint free cloth to clean
the read heads.
11. Pipette a 2.0μl aliquot of the calibration solution
onto the lower read head. Close the instrument
3. The Blank Solution Measurement icon will be
displayed. Open the instrument lid and pipette
a 2.0μl aliquot of the matrix blank solution
onto the lower read head.
Close the instrument lid and press the key
adjacent to the tick icon to initiate the blank
measurement.
4. The unit takes five readings at 260 and 330nm,
at both the 0.5 and 0.2mm pathlengths.
5. If the measured values are within the required tolerances, the Passes Test icon will be displayed. Press
the button adjacent to the Tick icon to continue or the button adjacent to the Cross icon to cancel
the calibration process.
6. The Calibration Solution Measurement icon
will be displayed. Open the instrument lid and
use a lint free cloth to clean the read heads.
7. Pipette a 2.0μl aliquot of the calibration
solution onto the lower read head. Close the
instrument lid and press the key adjacent to
the tick icon to initiate the calibration solution
measurement.
8. The unit takes five readings at 260 and 330nm,
at both the 0.5 and 0.2mm pathlengths.
9. If the measured values are within the required tolerances, the Passes Test icon will be displayed. Press
the button adjacent to the Tick icon to continue or the button adjacent to the Cross icon to cancel
the calibration process.
10. The Calibration Solution Measurement icon
will be displayed for a second time. Open the
instrument lid and use a lint free cloth to clean
the read heads.
11. Pipette a 2.0μl aliquot of the calibration
solution onto the lower read head. Close the
instrument lid and press the key adjacent to
the tick icon to initiate the calibration solution
measurement.
12. The unit takes five readings at 260 and 330nm, at both the 0.5 and 0.2mm path lengths.
13. If the measured values are within the required tolerances, the Passes Test icon will be displayed and
the instrument will calculate the new path length calibration factors. Press the button adjacent
to the Tick icon to continue or the button adjacent to the Cross icon to cancel the calibration
process.
14. The new calibration factor values must now be verified by re-measuring the air, blank and standard
values.
14
Page 17
12. The unit takes five readings at 260 and 330nm, at both the 0.5 and 0.2mm path lengths.
13. If the measured values are within the required tolerances, the Passes Test icon will be
displayed and the instrument will calculate the new path length calibration factors. Press the
button adjacent to the Tick icon to continue or the button adjacent to the Cross icon to cancel
the calibration process.
14. The new calibration factor values must now be verified by re-measuring the air, blank and
standard values.
15. The Air Measurement icon will be displayed.
Open the instrument lid and use a lint free
cloth to clean the read heads. Close the
instrument lid and press the key adjacent to
the tick icon to initiate the blank
measurement.
12. The unit takes five readings at 260 and 330nm, at both the 0.5 and 0.2mm path lengths.
13. If the measured values are within the required tolerances, the Passes Test icon will be
displayed and the instrument will calculate the new path length calibration factors. Press the
button adjacent to the Tick icon to continue or the button adjacent to the Cross icon to cancel
the calibration process.
14. The new calibration factor values must now be verified by re-measuring the air, blank and
standard values.
15. The Air Measurement icon will be displayed.
Open the instrument lid and use a lint free
cloth to clean the read heads. Close the
instrument lid and press the key adjacent to
the tick icon to initiate the blank
measurement.
16. The unit takes three dark and light readings at
260 and 330nm, at both the 0.5 and 0.2mm
pathlengths.
17. The Blank Solution Measurement icon will be
displayed. Open the instrument lid and pipette
a 2.0μl aliquot of the matrix blank solution
onto the lower read hea
d. Close the
instrument lid and press the key adjacent to
the tick icon to initiate the blank
measurement.
15. The Air Measurement icon will be displayed.
20. The Calibration Solution Measurement icon
will be displayed. Open the instrument lid and
use a lint free cloth to clean the read heads
Open the instrument lid and pipette a 2.0μl
aliquot of the calibration solution onto the
lower read head. Close the instrument lid and
press the key adjacent to the tick icon to
initiate the calibration solution measurement.
21. The unit takes three readings at 260 and 330nm, at both the 0.5 and 0.2mm pathlengths.
22. If the measured values are within the required tolerances, the Passes Test icon will be
displayed. Press the button adjacent to the Tick icon to continue or the button adjacent to the
Cross icon to cancel the calibration process
23. The instrument display will return to the micro
volume accessory settings screen and the
new path length calibration factors will be
shown.
20. The Calibration Solution Measurement icon
will be displayed. Open the instrument lid and
use a lint free cloth to clean the read heads
Open the instrument lid and pipette a 2.0μl
aliquot of the calibration solution onto the
lower read head. Close the instrument lid and
press the key adjacent to the tick icon to
Open the instrument lid and use a lint free cloth
to clean the read heads. Close the instrument
lid and press the key adjacent to the tick icon to
initiate the blank measurement.
16. The unit takes three dark and light readings at
260 and 330nm, at both the 0.5 and 0.2mm
pathlengths.
17. The Blank Solution Measurement icon will be
displayed. Open the instrument lid and pipette
a 2.0μl aliquot of the matrix blank solution onto
the lower read head. Close the instrument lid
and press the key adjacent to the tick icon to
initiate the blank measurement.
18. The unit takes three readings at 260 and 330nm,
at both the 0.5 and 0.2mm pathlengths.
19. If the measured values are within the required tolerances, the Passes Test icon will be displayed.
Press the button adjacent to the Tick icon to continue or the button adjacent to the Cross icon to
cancel the calibration process.
20. The Calibration Solution Measurement icon will
be displayed. Open the instrument lid and use
21. The unit takes three readings at 260 and 330nm, at both the 0.5 and 0.2mm pathlengths.
22. If the measured values are within the required tolerances, the Passes Test icon will be displayed.
Press the button adjacent to the Tick icon to continue or the button adjacent to the Cross icon to
cancel the calibration process.
a lint free cloth to clean the read heads Open
the instrument lid and pipette a 2.0μl aliquot
of the calibration solution onto the lower read
head. Close the instrument lid and press the key
adjacent to the tick icon to initiate the calibration
solution measurement.
23. The instrument display will return to the micro
volume accessory settings screen and the new
path length calibration factors will be shown.
15
Page 18
SECTION 6 – CLEANING AND DECONTAMINATION OF THE
MICRO VOLUME ACCESSORY
6.1 ACCESSORY CLEANING
Wiping the sample from both the upper and lower read heads upon completion of each sample
measurement with a lint free cloth is usually sufficient to prevent sample carryover and avoid residue
buildup. Although generally not necessary, water aliquots can be used to clean the measurement
surfaces after the measurement of particularly high concentration samples to ensure no residual sample
is retained on either read head. After measuring a large number of samples, however, it is recommended
that the areas around the upper and lower pedestals be cleaned thoroughly. This will prevent the wiping
after each measurement from carrying previous samples onto the measurement pedestals and affecting
low-level measurements. A final cleaning of all surfaces with deionised water is also recommended after
the user’s last measurement.
6.2 ACCESSORY DECONTAMINATION
If decontamination is necessary, a sanitising solution, such as a 0.5% solution of sodium hypochlorite
(1:10 dilution of common commercial bleach solutions – freshly prepared), can be used to ensure that
no biologically active material is present on the measurement read heads. The read head fittings are
made from stainless steel and are resistant to most common laboratory solvents. See Section 11 -Solvent
Compatibility for full details.
6.3 READ HEAD RECONDITIONING
The Bradford reagent as well as other buffers containing surfactants can “un-condition” the measurement
read head surfaces so that the liquid does not form a stable sample droplet. If this occurs, “buff” the
read head surfaces by rubbing each measurement surface aggressively with a dry laboratory wipe 30-40
times. This will “re-condition” the surface allowing the sample droplet to form.
SECTION 7 – ACCESSORIES
7.1 ACCESSORIES
Part Code Description of Accessory
035 092 DNA calibration standard
16
Page 19
SECTION 8 – MAINTENANCE AND SERVICE
8.1 ROUTINE MAINTENANCE
Ensure the external surfaces of the unit are clean and free from dust. The sample area should always be
kept clean and any accidental spillage should be wiped away immediately.
8.2 SERVICE
Our dedicated service team are on hand to help in the unlikely event that your Jenway equipment
develops a fault. Please contact them by one of the following means with a clear description of the
problem:
E-mail: service@bibby-scientific.com
Tel: +44 (0) 1785 810475
Fax: +44 (0) 1785 810471
On occasion it may be necessary for your equipment to be sent back to our Service Department for
repair. In this case please contact the Service Department for a reference number which you should
include with your faulty equipment. Please also ensure you include a clear description of the fault and a
completed copy of our Decontamination Certificate. This is available as a downloadable pdf file at www.
jenway.com, or contact us and we will be happy to fax you a copy. Please clearly mark the package for
the attention of the Service Department and post to the following address:
Bibby Scientific Ltd
Beacon Road
Stone
Staffordshire
ST15 0SA
United Kingdom
All replacement parts are guaranteed for 1 year and where ever possible, returned equipment is turned
around in 10 working days.
17
Page 20
SECTION 9 – TROUBLESHOOTING
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Calibration solution reading, 0.2mm path length, outside of
acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Calibration solution reading, 0.2mm path length, outside of
acceptable range
Duplicate calibration solution readings, 0.5mm path length,
outside of acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Calibration solution reading, 0.2mm path length, outside of
acceptable range
Duplicate calibration solution readings, 0.5mm path length,
outside of acceptable range
Duplicate calibration solution readings, 0.2mm path length,
outside of acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Calibration solution reading, 0.2mm path length, outside of
acceptable range
Duplicate calibration solution readings, 0.5mm path length,
outside of acceptable range
Duplicate calibration solution readings, 0.2mm path length,
outside of acceptable range
0.2mm path length factor outside of acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Calibration solution reading, 0.2mm path length, outside of
acceptable range
Duplicate calibration solution readings, 0.5mm path length,
outside of acceptable range
Duplicate calibration solution readings, 0.2mm path length,
outside of acceptable range
0.2mm path length factor outside of acceptable range
0.5mm path length factor outside of acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Calibration solution reading, 0.2mm path length, outside of
acceptable range
Duplicate calibration solution readings, 0.5mm path length,
outside of acceptable range
Duplicate calibration solution readings, 0.2mm path length,
outside of acceptable range
0.2mm path length factor outside of acceptable range
0.5mm path length factor outside of acceptable range
Blank solution reading, 0.5mm path length, outside of
acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Calibration solution reading, 0.2mm path length, outside of
acceptable range
Duplicate calibration solution readings, 0.5mm path length,
outside of acceptable range
Duplicate calibration solution readings, 0.2mm path length,
outside of acceptable range
0.2mm path length factor outside of acceptable range
0.5mm path length factor outside of acceptable range
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Calibration solution reading, 0.2mm path length, outside of
acceptable range
Duplicate calibration solution readings, 0.5mm path length,
outside of acceptable range
Duplicate calibration solution readings, 0.2mm path length,
outside of acceptable range
0.2mm path length factor outside of acceptable range
0.5mm path length factor outside of acceptable range
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Symbol
Issue
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Calibration solution reading, 0.2mm path length, outside of
acceptable range
Duplicate calibration solution readings, 0.5mm path length,
outside of acceptable range
Duplicate calibration solution readings, 0.2mm path length,
outside of acceptable range
0.2mm path length factor outside of acceptable range
0.5mm path length factor outside of acceptable range
Blank solution reading, 0.5mm path length, outside of
acceptable range
Blank solution reading, 0.2mm path length, outside of
acceptable range
Calibration solution reading, 0.5mm path length, outside of
acceptable range
Calibration solution reading, 0.2mm path length, outside of
acceptable range
9.1 ACCESSORY ERROR CODES
If an error code is displayed it will be accompanied by a clipboard icon and a number to indicate the
cause of the error. The first time that an error is displayed, it will be possible to repeat the erroneous
measurement. If the same error message is shown after the repeat measurement, the complete calibration process will need to be repeated. The table below shows the error codes:
9.1.1 Calibration Procedure Error Codes
Error Code Symbol Issue
1 Blank solution reading, 0.5mm path length, outside of acceptable range
2 Blank solution reading, 0.2mm path length, outside of acceptable range
3 Calibration solution reading, 0.5mm path length, outside of acceptable
range
4 Calibration solution reading, 0.2mm path length, outside of acceptable
range
5 Duplicate calibration solution readings, 0.5mm path length, outside of
acceptable range
6 Duplicate calibration solution readings, 0.2mm path length, outside of
acceptable range
7 0.2mm path length factor outside of acceptable range
8 0.5mm path length factor outside of acceptable range
9.1.2 Verification Check Error Codes
9 Blank solution reading, 0.5mm path length, outside of acceptable range
10 Blank solution reading, 0.2mm path length, outside of acceptable range
11 Calibration solution reading, 0.5mm path length, outside of acceptable
range
12 Calibration solution reading, 0.2mm path length, outside of acceptable
range
18
Page 21
9.2 TROUBLESHOOTING GUIDE
Issue Solution
Unable to achieve zero absorbance or Ensure that there is not a sample on the micro volume
100% transmittance when calibrating accessories read head.
Ensure the instrument lid is closed before and during
the calibration.
Ensure the lamp is working – if the lamp has failed
please request service assistance.
Unable to achieve a reading Ensure the correct path length is being used.
when measuring a sample
transmitted through the sample.
Ensure the lamp is working.
9.3 TECHNICAL SUPPORT
Jenway have a dedicated Technical Support team made up of experienced scientists who are on hand to
help with any applications advice and questions you may have about our products and how to use them.
If you require any technical or application assistance please contact the team at:
E-mail: jenwayhelp@bibby-scientific.com.
Phone: +44 (0)1785 810433
Fax: +44 (0)1785 810405
Ensure the sample isn’t too dense so that light is not
19
Page 22
SECTION 10 – GLOSSARY OF ICONS
Mode ICON Description
Common Back key
Common Tick icon - Done/yes
Common Cross icon – Cancel/no
Common Arrow icon – Move down, decrease
Common Arrow icon – Move up, increase
Settings 0.2mm path length setting / selected
Settings 0.5mm path length setting / selected
Settings Auto path length setting / selected
Settings Path length calibration
Settings 260nm Calibration standard absorbance value
Settings 330nm Calibration standard absorbance value
Settings Calibration standard path length value
Settings Micro volume accessory alignment
Calibration Air measurement
Calibration Blank solution measurement
20
Calibration Calibration solution measurement
Calibration Passes test
Calibration Fails test
Page 23
SECTION 11 – CHEMICAL COMPATIBILITY
Assay Chemical Concentration
BCA Sodium bicinchoninate 1%*
BCA Sodium carbonate 2%*
BCA Sodium tartrate 0.16%*
BCA Sodium hydroxide 0.1M*
BCA Sodium bicarbonate 0.95%*
BCA Copper (II) sulphate 0.08%
Biuret Sodium potassium tartrate 0.9%*
Biuret Copper (II) sulphate 0.3%*
Biuret Potassium iodide 0.5%*
Biuret Sodium hydroxide 0.08M
Lowry Sodium carbonate 1.6%
Lowry Copper (II) sulphate 0.032%
Lowry Sodium potassium tartrate 0.016%
Lowry Sodium dodecyl sulphate 0.08%
Lowry Sodium hydroxide 0.08M
Lowry Folin reagent (lithium and sodium 0.04N*
molybdotungstophosphate solution)
Bradford Coomassie brilliant Blue G-250 0.01%*
Bradford Ethanol 4.75%*
Bradford Phosphoric acid 8.5%*
Bradford Sodium hydroxide 0.1M
DMSO 10%
Acetonitrile OK
Methanol OK
2-Propanol OK
*Highest concentration
21
Page 24
UK
Bibby Scientific Ltd.
Beacon Road, Stone
Staffordshire ST15 0SA
United Kingdom
Tel: +44 (0)1785 812121
Fax: +44 (0)1785 810405
e-mail: sales@bibby-scientific.com
www.bibby-scientific.com
North & South America
Bibby Scientific US Inc.
t/a Techne Inc.
3 Terri Lane, Suite 10
Burlington, NJ 08016 USA
Toll Free (in NA): 800-225-9243
Tel: +1 609 589 2560
Fax: +1 609-589-2571
e-mail: labproducts@techneusa.com
www.techneusa.com
Middle East
Bibby Scientific Middle East Ltd.
PO Box 27842, Engomi 2433
Nicosia
Cyprus
Tel: +357 22 660 423
Fax: +357 22 660 424
e-mail: sales@bibby-scientificme.com
France
Bibby Scientific Limited
Bâtiment Le Deltaparc Parc Silic PN2
7 rue du Canal
BP 55437 VILLEPINTE
95944 ROISSY Charles de Gaulle
France
Tel: +33(0)148 63 78 03
Fax: +33(0)148 63 78 01
e-mail: ventes@bibby-scientific.com
www.bibby-scientific.com
Italy
Bibby Scientific Italia Srl
Via Alcide de Gasperi 56
20077 Riozzo di Cerro al Lambro
Milano Italia
Tel: +39 (0)2 98230679
Fax: +39 (0)2 98230211
e-mail: marketing@bibby-scientific.it
www.bibby-scientific.it
Asia
Bibby Scientific - Singapore
Prudential Tower, Level 26
30 Cecil Street
Singapore 049712
Tel: +65 6631 2976
Fax: +44 (0)1785 810405
e-mail: info@bibby-scientific.com
www.bibby-scientific.com
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