Jenway 7200 Instruction Manual

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Spectrophotometer Model 7200

Operating Manual

720 005 REV A 07/13

Safety

Please read this information carefully prior to installing or using this equipment.

1. The unit described in this manual is designed to be operated only by trained personnel. Any adjustments, maintenance and repair must be carried out as defined in this manual, by a person qualified to be aware of the hazards involved.

2. It is essential that both operating and service personnel employ a safe system of work, in addition to the detailed instructions specified in this manual.

3. Other than for those items defined in the maintenance procedures herein there are no user serviceable items in this instrument. Removal of covers and attempted adjustment or service by unqualified personnel will invalidate the warranty and may incur additional charges for repair.

4. References should always be made to the Health and Safety data supplied with any chemicals used. Generally accepted laboratory procedures for safe handling of chemicals should be employed. Do not use hazardous or flammable substances in the instrument.

5. If it is suspected that safety protection has been impaired in any way, the unit must be made inoperative and secured against any intended operation. The fault condition should immediately be reported to the appropriate servicing authority.

6. The warning symbol alerts the user to important information about using the instrument. Read and follow the associated instructions carefully.

7. This instrument uses a UV light source. Do not look directly at the light source.

8. WARNING: If the equipment is not used in the manner specified, the protection provided by the equipment may be impaired.

9. Do not replace the detachable mains leads with inadequately rated leads.

Merci de lire attentivement ces informations avant d’installer ou d’utiliser cet appareil.

1. L’appareil décrit dans ce manuel est conçu pour être utilisé uniquement par des personnes formées. Tout réglage, maintenance ou réparation doit être effectué comme décrit dans ce manuel, par une personne qualifiée consciente des risques encourus.

2. Il est essentiel que les personnes utilisant et intervenant sur cet appareil respectent les règles de sécurité de travail, en plus des instructions détaillées précisées dans ce manuel.

3. En-dehors des éléments décrits dans les procédures de maintenance ci-incluses, cet appareil ne contient aucun élément réparable par l’utilisateur. L’enlèvement des capots et les tentatives de réglage ou de réparation par des personnes non qualifiées invalide toute garantie et entraîne un risque de frais de réparation supplémentaires.

4. Toujours se référer aux fiches techniques de santé et de sécurité accompagnant tout produit chimique utilisé. Respecter les procédures de laboratoire généralement acceptées pour la manipulation en toute sécurité des produits chimiques. Ne pas utiliser de substances dangereuses ou inflammables sur l’appareil.

5. Si l’utilisateur suspecte qu’un problème quelconque puisse mettre en cause la sécurité, l’appareil doit être rendu inopérant en empêchant son utilisation. Communiquer la défaillance constatée au service de maintenance compétent.

6. Le symbole d’alerte signale à l’utilisateur les informations importantes concernant l’utilisation de l’appareil. Lire et suivre les instructions fournies avec la plus grande attention.

7. Cet appareil utilise une source lumineuse UV. Ne pas regarder directement vers la source.

8. ATTENTION. Si l’appareil n’est pas utilisé de manière adéquate, la protection de l’appareil pourrait être impactée.

9. Ne pas remplacer le cordon d’alimentation fourni par un cordon d’alimentation de dimension électrique non adapté.

Bitte lesen Sie diese Hinweise vor Installation oder Gebrauch dieser Ausrüstung sorgfältig durch.

1. Das in diesem Handbuch beschriebene Gerät darf nur von geschultem Personal bedient werden. Alle Anpassungen, Wartungsarbeiten und Reparaturen müssen entsprechend der Vorgaben in diesem Handbuch und von einer kompetenten Person, die mit den damit verbundenen Gefahren vertraut ist, durchgeführt werden.

2. Es ist wichtig, dass sowohl das Bedienungs- als auch das Service-Personal zusätzlich zu den detaillierten Anweisungen in diesem Handbuch ein sicheres Arbeitssystem einsetzen.

3. Mit Ausnahme der Teile, deren Wartungsverfahren in diesem Handbuch beschrieben sind, enthält dieses Gerät keine weiteren Teile, die vom Benutzer gewartet werden können. Das Entfernen von Abdeckungen und Versuche von hierfür unqualifiziertem Personal, Anpassungen oder Wartungsarbeiten durchzuführen, haben zur Folge, dass die Garantie verfällt und können zusätzliche Reparaturkosten auslösen.

4. Es ist jederzeit auf die sicherheitsrelevanten Daten sämtlicher verwendeter Chemikalien Bezug zu nehmen. Allgemein anerkannte Labormethoden zum sicheren Umgang mit Chemikalien sollten eingesetzt werden. Verwenden Sie keine gefährlichen oder entzündlichen Stoffe in Verbindung mit dem Gerät.

5. Besteht der Verdacht, dass die Sicherheitsvorrichtungen in irgendeiner Weise beschädigt wurden, muss das Gerät außer Betrieb genommen und gegen weiteren Gebrauch gesichert werden. Die Störung sollte der zuständigen Serviceeinrichtung unverzüglich gemeldet werden.

6. Das Warnsymbol weist auf wichtige Informationen zur Verwendung des Geräts hin. Lesen und befolgen Sie die dazugehörigen Anweisungen sorgfältig.

7. Dieses Instrument greift auf eine UV-Lichtquelle zurück. Nicht direkt in die Lichtquelle schauen.

8. ACHTUNG: Wenn das Gerät nicht in der vorgegebenen Weise eingesetzt wird, können die Schutzfunktionen des Gerätes beeinträchtigt werden.

9. Abnehmbares Anschlusskabel nicht durch unangemessen bewertete Kabel austauschen.

Leggere attentamente queste istruzioni prima di installare o utilizzare il dispositivo.

1. L’unità descritta nel presente manuale è stata realizzata per essere utilizzata solo da personale che ha ricevuto l’apposita formazione. Qualsiasi operazione di regolazione, manutenzione e riparazione deve essere effettuata sulla base di quanto indicato nel presente manuale da personale qualificato consapevole dei rischi connessi.

2. È fondamentale che il personale operativo e il personale addetto alla manutenzione utilizzino un sistema di lavoro sicuro, oltre a seguire le istruzioni specificate nel presente manuale.

3. Oltre a quelli indicati nelle procedure di manutenzione, all’interno di questo dispositivo non sono presenti altri elementi sui quali è possibile effettuare interventi. La rimozione delle protezioni e qualsiasi tentativo di regolazione o di manutenzione posto in essere da personale non qualificato invaliderà la garanzia. In questi casi, sarà necessario pagare un importo per le riparazioni effettuate.

4. È sempre necessario fare riferimento ai dati sulla salute e sulla sicurezza forniti con le sostanze chimiche utilizzate. Adottare le procedure di laboratorio generalmente accettate per la gestione delle sostanze chimiche. Non utilizzare sostanze pericolose o infiammabili sullo strumento.

5. Nel caso in cui si sospetti che la salute possa essere pregiudicata in qualsiasi modo, disattivare l’unità per renderla inutilizzabile. Qualsiasi condizione di errore deve essere immediatamente segnalata al responsabile per la manutenzione.

6. Il simbolo di avvertenza informa l’utente sulle informazioni importanti in merito all’uso dello strumento. Leggere e seguire le istruzioni corrispondenti con cura.

7. Questo strumento utilizza una sorgente di luce UV. Non guardare direttamente la sorgente di luce.

8. AVVERTENZA: qualora il dispositivo non venga utilizzato nel modo descritto, la protezione fornita dal dispositivo stesso potrebbe risultare compromessa.

9. Non sostituire i cavi di alimentazione di rete scollegabili con cavi inadeguati.

Lea esta información atentamente antes de instalar o utilizar este equipo.

1. La unidad descrita en este manual está diseñada para que solamente la utilice personal con formación. Cualquier operación de ajuste, mantenimiento y reparación debe llevarse a cabo del modo indicado en este manual y debe realizarla una persona cualificada que sea consciente de los peligros que implica.

2. Es fundamental que tanto los operarios como el personal de servicio utilicen un sistema de trabajo seguro, así como las instrucciones detalladas que se especifican en este manual.

3. Cualquier elemento que no se encuentre entre los definidos en los procedimientos de mantenimiento aquí descritos no podrá utilizarse en este instrumento. La extracción de las tapas y los intentos de ajuste o reparación por parte de personal no cualificado invalidarán la garantía y pueden incurrir en cargos adicionales por reparación.

4. Siempre deberían consultarse los datos sobre Salud y Seguridad que se suministran con cualquier producto químico que se utilice. Es necesario llevar a cabo los procedimientos de laboratorio de aceptación generalizada para la manipulación segura de productos químicos. No utilice sustancias peligrosas o inflamables en el instrumento.

5. Si existe la sospecha de que las medidas protectoras de seguridad han quedado dañadas en cualquier modo, la unidad debe inutilizarse y protegerse contra toda operación que se intente llevar a cabo. El estado de fallo debe comunicarse inmediatamente a la autoridad de servicio de mantenimiento y reparación pertinente.

6. El símbolo de advertencia avisa al usuario de información importante relacionada con el uso del instrumento. Lea atentamente y siga las instrucciones correspondientes.

7. Este instrumento utiliza una fuente de luz UV. No mire directamente a la fuente de luz.

8. ADVERTENCIA: Si el equipo no se utiliza de la manera especificada, la protección que ofrece el aparato puede verse afectada.

9. No sustituya el cable de alimentación eléctrica con cables de voltaje inadecuado.

Contents

Page

Safety 3

SECTION 1 - Introduction 11

1.1 Instrument description 11

1.2 Instrument specification 12

SECTION 2 - Installation 13

2.1 Unpacking 13

2.2 Installation 13

2.3 Rear panel 14

2.4 Front access panel 14

2.5 Lamp access panel 15

2.6 Display 15

SECTION 3 - Theory and practice of spectroscopy measurements 16

3.1 Theory of spectroscopy measurement 16

3.2 Spectroscopy measurement 17

3.3 Good practice guidelines 17

SECTION 4 - Instrument setup 19

4.1 Navigating and screen setup 19

4.2 Instrument settings screen 19

4.3 Printing and saving 20

4.4 Heated cell accessory control 21

4.5 Date and time 22

4.6 Language 23

4.7 Software update 23

SECTION 5 - Single wavelength 25

5.1 Simple (ABS/%T) measurement mode 26

5.1.1 Method set up 26

5.1.2 Selecting a wavelength 26

5.1.3 Measurement name 27

5.1.4 Blank calibration and sample measurement 27

5.2 Optical density measurement mode 28

5.2.1 Method setup 28

5.2.2 Selecting a wavelength 29

5.2.3 Measurement name 29

5.2.4 Entering a factor 29

5.2.5 Entering concentration units 30

5.2.6 Blank calibration and sample measurement 30

5.3 Use a factor measurement mode 31

5.3.1 Method setup 32

5.3.2 Selecting a wavelength 32

5.3.3 Measurement name 32

5.3.4 Entering a factor 33

5.3.5 Units of measure 33

5.3.6 Blank calibration and sample measurement 33

5.4 Use standard(s) measurement mode 35

5.4.1 Method setup 35

5.4.2 Selecting a wavelength 36

5.4.3 Measurement name 36

5.4.4 Entering concentration units 36

5.4.5 Selecting the number of standards and replicates 37

5.4.6 Measuring standards 37

5.4.7 Load standard curve 39

5.5 Blank calibration and sample measurement 40

SECTION 6 - Spectrum 42

6.1 Method setup 42

6.1.1 Measurement name 42

6.1.2 Selecting measurement mode 43

6.1.3 Setting start and end wavelengths 43

6.2 Blank calibration and sample measurement 44

6.3 Data analysis 45

6.3.1 Spectrum zoom 45

6.3.2 Spectral points analysis 46

SECTION 7 - Kinetics 47

7.1 Method setup 47

7.1.1 Measurement name 47

7.1.2 Selecting number of wavelengths 48

7.1.3 Selecting measurement mode 48

7.1.4 Run time 48

7.1.5 Lag time (seconds) 48

7.1.6 Selecting concentration units 49

7.1.7 Selecting a wavelength and factor 49

7.2 Blank calibration and sample measurement 50

7.3 Post measurement analysis 51

SECTION 8 - Glossary of icons 52

SECTION 9 - Accessories and spare parts 54

9.1 Optional accessories 54

9.2 Connecting the accessories 54

9.2.1 External printer 54

9.2.2 Passive accessories 54

9.2.3 Active accessories 55

9.3 Spares 56

SECTION 10 - Maintenance and service 57

10.1 Routine maintenance and cleaning 57

10.2 Lamp replacement 57

10.2.1 Halogen lamp module replacement 57

10.3 Firmware update procedure 59

10.4 Service 59

SECTION 11 - Troubleshooting 60

11.1 Error codes 60

11.2 Troubleshooting guide 64

11.3 Technical support 64

SECTION 12 - Declaration of conformity 65

SECTION 1 - Introduction

1.1 INSTRUMENT DESCRIPTION

Model 7200 is a visible diode array spectrophotometer which is suited to a wide range of applications in education, quality control, environmental and clinical analysis. This spectrophotometer covers a wavelength range from 335nm to 800nm, with measurement modes for photometrics, concentration, spectrum scanning, quantitation and kinetics. This spectrophotometer has a touchscreen user interface for improved navigation.

1.2 INSTRUMENT SPECIFICATION

7200

Wavelength Range 335 to 800nm Accuracy ± 2nm Repeatability ± 2nm Spectral bandwidth 7nm

Photometrics

Absorbance -0.300 to 2.500A Accuracy +/- 0.01A at 1.0A and 546nm Stability (A) +/- 0.005A/h at 0.04A and 546nm Noise +/- 0.002A at 0.04A and +/- 0.02A at 2.0A and 546nm Stray Light at 340nm, %T <1%T according to ANSI/ASTM E387-72 Concentration Range +/- 2500 Calibration Blank with a single standard or factor Factor +/- 1000 Standard +/- 1000

Optical Density

Factor +/- 1000 Quantitation Range +/- 2500 Calibration Blank with up to 6 standards Curve fit algorithms Linear and linear through zero Kinetics Measurement Time 15 to 9999 seconds Number of wavelengths 3 Calibration Blank with a factor Display Graphical and concentration Analysis Concentration Spectrum Range 335 to 800nm Analysis Absorbance or % transmittance and up to 50 spectral

analysis points

Other

Beam height 15mm Light source Tungsten Halogen lamp Results memory Limited by attached USB memory stick Outputs USB x 2 Supply voltage/frequency 100 – 240VAC at 50 to 60Hz Power supply 12V DC, 3.8A Size (w x d x h) 212 x 422 x 120mm Weight 2.8kg Warranty 2 years on the instrument, 1 year on the lamp

SECTION 2 - Installation

2.1 UNPACKING

Remove the 7200 from the packaging and ensure the following items are included:

1. Model 7200 spectrophotometer (720 001)

2. 12V 3.8A power supply unit (M7980)

3. Mains Power leads UK plug (M7817UK), EU (M7817X6) and US (M7817X1)

4. Instruction manual (720 005)

5. Jenway Foreign Manual CD (JENMANCD)

2.2 INSTALLATION

Model 7200 is supplied ready to use.

The unit should be placed on a clean flat surface which is free from drafts and vibrations. Do not position the equipment so that it is difficult to access the power switch or disconnect the power supply unit from the mains supply.

The unit should only be used in an environment with a temperature range of 5 to 40°C and maximum relative humidity 0 to 80% for temperatures up to 31°C, decreasing linearly to 50% at 40°C. When the instrument is used for the first time or moved to a different environmental temperature, it is important to allow the instrument to equalise to the ambient temperature. Therefore allow the unit to stand for 2 hours before switching it on.

The supplied power supply unit is designed for operation on 100vAC to 240vAC input at 50 to 60Hz. Select the power lead and attach to the power supply unit. Connect the power supply unit to the power inlet socket on the rear panel of the instrument and connect to the mains socket. Ensure that the sample chamber is empty before turning the power on at the mains and switching the instrument on using the power switch on the rear of the instrument.

The instrument will perform several power-on tests and lamp calibration before displaying the main screen.

WARNING: Leaving cuvettes in the sample holder during power up will result in failure of the power on tests.

2.3 REAR PANEL

3 2

The image below shows the rear panel on the instrument:

Fig. 2.3.1 – Rear Panel

1. USB port Allows connection to a USB printer

2. PC connector Allows connection to a PC

3. Power in socket Connection socket for power supply unit

4. Rocker Switch Allows the unit to be powered on or off

3 2

1. Cuvette holders (10 x 10mm) For cuvette storage

2. Instrument lid Provides access to

sample chamber

3. Touchscreen Display User interface

4. USB memory stick slot Accepts USB stick

The image below shows the rear panel on the instrument:

Fig. 2.3.1 – Rear Panel

1. USB port Allows connection to a USB printer

2. PC connector Allows connection to a PC

3. Power in socket Connection socket for power supply unit

4. Rocker Switch Allows the unit to be powered on or off

2.4 FRONT PANEL

The image below shows the front panel of the instrument:

1. Cuvette holders (10 x 10mm) - For cuvette storage

2. Instrument lid - Provides access to sample chamber

3. Touchscreen Display - User interface

4. USB memory stick slot - Accepts USB stick

Fig. 2.4.1 – Front Panel

2.5 LAMP ACCESS PANEL

The image below shows the lamp access panel on the underside of the instrument:

Fig. 2.5.1 – Lamp Access Panel

1. Lamp Access Panel

Allows access to lamp when replacement is necessary

WARNING! Refer to section 10.2 for instructions on how to remove and replace the lamp.

The image below shows the lamp access panel on the underside of the instrument:

WARNING! Refer to section 10.2 for instructions on how to remove and replace the lamp.

2.6 DISPLAY

The instrument has a touchscreen display which enables easy setup and navigation of the instrument.

Fig. 2.6.1 – Main Screen

• Settings

• Single Wavelength Measurement Modes

• Spectrum Measurement Mode

• Kinetics Measurement Mode

SECTION 3 – Theory and Practice of Spectroscopy Measurements

A =  l c

-1cm-1

)

-1

)















Where:

is the incident light

is the transmitted light

 is the path length

-1

)













is the incident light

3.1 THEORY OF SPECTROSCOPY MEASUREMENT

UV-visible spectroscopy is the measurement of the absorbance of light at a speciﬁc wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample. The Beer-Lambert law is used to relate the absorption of light to the properties of the sample through which the light is travelling through. The Beer-Lambert law states that:

A = l c

A is the absorbance

-1cm-1

is the molar absorption coefﬁcient (l mol

c is the concentration (mol l

-1

)

l is the path length (cm)

This law shows that absorbance is linear to concentration but this is only true for low concentrations. For absorbance levels above 3 the concentration starts to move away from the linear relationship.

Transmittance is the proportion of the light which passes through the sample:

Where:

is the incident light

lt is the transmitted light l is the path length

Therefore:

Absorbance is inversely related to transmittance:

3.2 SPECTROSCOPY MEASUREMENT

There are four main components of a spectrophotometer. These are a light source to emit a high and constant amount of energy over the full wavelength range, a sample holder, a method for separating the light into discreet wavelengths and a light detector.

The optical layout of the 7200 spectrophotometer is shown below:

The whole light spectrum from the pre-aligned tungsten halogen lamp and the LED is collimated through a lens and passed through the sample. The spectrum of light then passes through a further slit and lens arrangement before being focussed onto the diffraction grating, which separates the light into discrete wavelengths. The light which has not been absorbed by the sample is measured by the photodiode array detector. The photo-diode array detector used is mounted directly onto the detector PCB and the output is used to calculate the % transmittance. The result is displayed either as % transmittance or absorbance on the instrument display.

3.3 GOOD PRACTICE GUIDELINES

1. For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in use ambient temperature and light levels should remain as constant as possible.

2. If required adherence to Standard Operating Procedures (SOP) and Good Laboratory Practice (GLP) should be monitored with regular calibration checks and a suitable Quality Control (QC) programme.

3. Ensure that there is nothing additional in the sample chamber which could block the light path during calibration and sample measurement. Do not divert the light path using a mirrored surface within the sample compartment.

Figure 3.2.1 – Diagram of light path

4. The correct selection of sample containers is imperative for accurate and reproducible results:

a) Check that the material of the sample container is compatible with the wavelengths to be used for measurement. In general glass can only be used down to 360nm or 320nm depending on quality. Standard plastic cuvettes can be used down to 320nm. Special UV versions can be used down to 260nm. Below this level quartz cuvettes must be used.

b) Plastic disposable cuvettes should only be used ONCE.

c) Glass cuvettes should be thoroughly cleaned after use. Discard when scratches become evident on optical surfaces.

d) Care should be taken when selecting semi-micro or micro cuvettes. The cuvette window on the inner chamber (the area ﬁlled with sample) must be wider than the aperture in the sample holder or light will reach the detector without passing through the sample. In this case, semi micro or micro cuvettes with self-screening black surrounds must be used or, alternative holders for these cuvettes should be used.

e) Glass test tubes and other sample tubes should be used with care. Where possible, matched tubes should be used and any index mark set to the correct position before measurements are made.

f) Ensure any sample containers used are compatible with the constituents of both the samples and standards they are to hold. Plastic cuvettes are not compatible with organic solvents.

g) All sample containers must be handled with care; by the top, bottom and non-optical surfaces only. Any ﬁnger marks evident must be removed by a suitable cleaning process.

h) Flow-through cuvettes must be selected with care and consideration for the sample type, sample volume, pumping system, rinse, sample and waste handling to be used.

5. Samples and standards should not be stored in open cuvettes or sample containers as evaporation will change the value and lead to staining of the walls which may be irreversible. If stored in stoppered and sealed cuvettes, they should be ﬁlled with little or no air space and the values regularly checked against a reference standard or quality control material.

6. Samples should be allowed to equilibrate to ambient temperature before measurement (unless a suitable temperature controlled sample holder is in use). Temperature change during measurement may cause air bubbles to form on the walls of the sample holder. This is a common cause of drift during measurement.

7. In the preparation of samples and standards high grade borosilicate glass and AR grade chemicals and reagents must be used. Good quality deionised water or other suitable solvents must be used for dissolving or diluting samples, chemicals and reagents.

8. All measurements require calibration to a blank, for maximum accuracy this should be prepared with care using the same deionised water or solvent used for dissolving or diluting the sample. Where reagents are added to the sample to produce a colour proportional to its concentration a ‘sample based’ blank should be used. In this case the blank should consist of all reagents or chemicals to be used, except the sample which will produce the colour to be measured.

9. Deviations from the Beer-Lambert Law may occur at high and low concentrations giving non-linear response during sample concentration measurements. For all new methods a linear range should be deﬁned by the preparation of a calibration curve. The quantitation mode may be used to construct such a curve against which sample results are automatically measured.

10. Cuvettes and sample holders must be ﬁlled to a minimum level which covers the light path. All Jenway spectrophotometers have a beam height of 15mm.

11. The instrument must be calibrated to zero absorbance/100% transmittance prior to taking readings. In the spectrum measurement mode a baseline scan must be performed before performing a sample scan.

SECTION 4 – Instrument Setup

4.1 NAVIGATING AND SCREEN SETUP

The main home screen is displayed below:

Model 7200 has a colour touchscreen user interface. To navigate around the spectrophotometer screen,

touch the icon or text which you want to action.

In every screen there is a back arrow which returns to the previous screen without saving any

changes.

In each measurement mode there is a home icon which returns immediately to the home screen.

4.2 INSTRUMENT SETTINGS SCREEN

The instrument settings screen is accessed by touching the instrument settings icon in the Home Screen. This screen enables access to printing and saving options, heated cell accessory control, setting date and time, language and software updates. Touch the down arrow to view the second screen.

4.3 PRINTING AND SAVING

Printing (with the accessory printer) and saving (to a USB memory stick) can be performed manually in each mode but for selected measurement modes it can also be set up be carried out automatically. Automatic saving can only be done in the single measurement modes.

Touch Printing or Saving to toggle between Manual and Automatic.

To print or save manually in any measurement mode, touch the overﬂow icon button at the bottom of

the screen.

Touching the overﬂow icon displays the options for manual printing or manual saving. Touch the save

icon to save the result to USB memeory stick. If a USB memory stick is not inserted an error message will be displayed. Touch the print icon to print the results using an external printer. If the printer is not connected, switched on or out of paper an error message will be displayed. Touching the overﬂow

icon

icon once again will cycle the screen back to the previous view, allowing blank and sample measurements

to be performed.

Once a sample reading has been performed if the results are not saved to USB memory stick a warning

message will be displayed when the mode is exited by pressing the Home icon.

Touch Continue to exit the measurement mode and the results will not be saved. Touch Return to

return to the measurement mode so that the results can be saved.

4.4 HEATED CELL ACCESSORY CONTROL

A heated 10x10mm cuvette holder is available as an optional accessory for Model 7200. This accessory

enables the temperature of the sample to be adjusted. To set up the parameters of the heated cell

accessory, touch Heated Cell in the Settings screen. Touch Use Heated Cell to toggle between Off and

On.

To adjust the temperature of the heated cell, touch Heated Cell Temperature. Touch the up or down

arrows to increase or decrease the set temperature in 0.5°C increments. The minimum temperature is

32°C, the maximum temperature is 42°C. Touch OK to conﬁrm or touch the back arrow to return to

the previous screen without saving any changes. Please note that the heated cell takes approximately 30

mins to heat a 2.5ml sample to 37°C.

4.5 DATE AND TIME

The time and date screen enables the current time and date to be set. This information will be saved on

all results and displayed on printouts.

To adjust the date touch Date and use the arrow icons to increase or decrease the number. Touch OK to conﬁrm or touch the back arrow to return to the previous screen without saving any changes.

To adjust the time touch Time and use the arrow icons to increase or decrease the number. Touch OK to conﬁrm or touch the back arrow to return to the previous screen without saving any changes.

The date format can be displayed as either European format dd/mm/yy or American format mm/dd/yy

by touching Date Format. To display the time as either a 12 hour clock or a 24 clock touch Time

Format.

4.6 LANGUAGE

The 7200 operating software can also be displayed in French. To select the required language touch Language. The software will change into French language. To return to English touch Langue. The language selected will also be displayed on the printouts. The selected language when the instrument is switched off will be loaded when the instrument is turned on.

4.7 SOFTWARE UPDATE

Update Software

The 7200 operating software can be updated via USB memory stick. The update must ﬁrst be downloaded to a USB memory stick from the Jenway website. Insert a USB device into the USB port on the front of the instrument and navigate to the Update Software option in the settings screen. Touch Update Software to begin the update.

If there is no USB memory stick inserted

the above screen will be shown.

If there is no update ﬁle on the USB memory stick the above screen will be shown.

If there is a valid update ﬁle on the USB memory stick, then Load Software becomes active.

Touch Load Software to start the upload process.

Touch Update to begin installing the new software.

At the end of Part 5 of 5, the instrument will restart and the bootloader will perform the update.

Once the update is completed touch Continue to complete the update process.

SECTION 5 – Single Wavelength

The single wavelength measurement mode provides options to measure simple absorbance and %

transmittance, optical density and concentration using a factor, single standard or quantitation curve.

Touch Simple Wavelength to display the different measurement modes:

Simple (Abs/%T)

This option allows simple measurements of absorbance or % transmittance to be performed.

Optical Density

Optical density measurement mode enables optical density measurements of cell cultures and broths

to be performed. In this mode, it is possible to use a known factor to estimate the number of cells

present.

Use a factor

This measurement mode enables concentration measurements to be performed. It is possible to use a

known factor to determine the concentration of a solution based on the measured absorbance.

Use standard(s)

This mode enables sample concentrations to be calculated using a standard curve or a single standard.

In this mode, a number of standard solutions covering a range of known concentrations are measured

at a set wavelength. The absorbance or % transmittance of these solutions are plotted to create a

standard curve with replicate standard measurements, if required. Once the standard curve has been

created, a sample of unknown concentration can be measured and the concentration calculated using

the standard curve. If only one standard is available the instrument will subsequently calculate the factor

which can be used to calculate the unknown concentration.

5.1 SIMPLE (ABS/%T) MEASUREMENT MODE

5.1.1 Method Setup

Simple (Abs/%T) enables simple measurements of absorbance and % transmittance to be performed. The sample is measured at every wavelength but only the result for the selected wavelength is displayed. There are no post measurement calculations available in this measurement mode. Touch Simple (Abs/%T) to enter this measurement mode and begin method setup.

The method parameters which can be selected are the wavelength and the measurement name. Once these have been entered touch the forwards arrow to enter the measurement screen. The back arrow will return to the previous screen and the home icon will return to the home screen. Neither of these options will save any parameters entered.

5.1.2 Selecting a Wavelength

Touch Wavelength (nm) to enter the required wavelength. Either type the wavelength required using the numeric keypad or touch delete to clear the screen and then type in the wavelength. Touch OK to conﬁrm and save or touch the back arrow to return to the previous screen without saving the entered wavelength.

5.1.3 Measurement Name

Touch Measurement Name to enter the required name for the sample. This name will be used to identify the result on any printouts or if the result is saved to USB memory stick. Either type the name required using the alphanumeric keypad or touch delete to clear the screen and then type in the name.

Please note the name is restricted to a maximum of 8 characters. Touch OK to conﬁrm and save or touch the back arrow to return to the previous screen without saving the entered name. The ABC icon will toggle between alpha and numeric options.

5.1.4 BLANK CALIBRATION AND SAMPLE MEASUREMENT

A blank calibration must be performed before a sample can be measured. Insert a cuvette containing

the blank solution into the sample chamber. Touch Blank and the instrument will perform a zero

absorbance and 100% transmittance reading.

Once the blank calibration is complete Read becomes active and the sample can be measured. As

the spectrophotometer measures the entire wavelength range in one scan, if the wavelength is

adjusted before a sample is measured the instrument doesn’t need be calibrated again at the new

wavelength.

Remove the cuvette containing the blank solution and place a cuvette containing the sample to be

measured in the sample holder. Touch Read to measure the sample. Once the measurement is

complete the photometric result will be shown on the screen. Subsequent samples can be measured

in the same way.

To adjust the wavelength there is no need to return to the method setup. Touch the screen to open

the adjustment screen. Touch the arrow icons to increase or decrease the wavelength. The

photometrics result will be adjusted accordingly. Touch the back arrow to return to the measurement

screen and the adjusted results will be displayed on the screen.

5.2 OPTICAL DENSITY MEASUREMENT MODE

The OD 600 measurement mode enables optical density measurements of cell cultures and broths to

be performed. In this measurement mode it is possible to use a known factor to estimate the number of

cells. There are no post measurement calculations available in this measurement mode. Touch Optical

Density to enter this measurement mode and begin method setup.

5.2.1 Method Setup

The method parameters which can be selected are the wavelength, the measurement name, the factor and the concentration units. Once these have been entered touch the forwards arrow to enter the measurement screen. The back arrow will return to the previous screen and the home icon will return to the home screen. Neither of these options will save any parameters entered.

5.2.2 Selecting a Wavelength

Touch Wavelength (nm) to enter the required wavelength. Either type the wavelength required using

the numeric keypad or touch delete to clear the screen and then type in the wavelength. Touch OK to

conﬁrm and save or touch the back arrow to return to the previous screen without saving the entered

wavelength.

5.2.3 Measurement Name

Touch Measurement Name to enter the required name for the sample. This name will be used to

identify the result on any printouts or if the result is saved to USB memory stick. Either type the name

required using the alphanumeric keypad or touch delete to clear the screen and then type in the

name.

Please note the name is restricted to a maximum of 8 characters. Touch OK to conﬁrm and save or

touch the back arrow to return to the previous screen without saving the entered name. The ABC icon

will toggle between alpha and numeric options.

5.2.4 Entering a factor

Touch Factor to enter the required factor. The minimum factor which can be entered is

-1000, the maximum factor which can be entered is +1000. Either type the factor required using the numeric keypad or touch delete to clear the screen and then type in the factor. Touch OK to conﬁrm and save or touch the back arrow to return to the previous screen without saving the entered factor.

5.2.5 Entering concentration units

The standard units of measure for optical density is cells/ml but these can be adjusted depending on requirements. Touch Units to adjust the concentration units. Please note that this is just a text ﬁeld and changing within a unit family e.g. mg/l and g/l will not change the concentration result.

Either type the units required using the alphanumeric keypad or touch delete to clear the screen and

then type in the units. Please note the units are restricted to a maximum of 8 characters. Touch OK

to conﬁrm and save or touch the back arrow to return to the previous screen without saving the

entered units. The 123 icon will toggle between alpha and numeric options.

5.2.6 BLANK CALIBRATION AND SAMPLE MEASUREMENT

A blank calibration must be performed before a sample can be measured. Insert a cuvette containing

the blank solution into the sample chamber. Touch Blank and the instrument will perform a zero

absorbance and 100% transmittance reading.

Once the blank calibration is complete Read becomes active and the sample can be measured. As

the spectrophotometer measures the entire wavelength range in one scan, if the wavelength is

adjusted before a sample is measured the instrument doesn’t need to be calibrated again at the new

wavelength.

Remove the cuvette containing the blank solution and place a cuvette containing the sample to be

measured in the sample holder. Touch Read to measure the sample. Once the measurement is

complete the result will be shown on the screen. Subsequent samples can be measured in the same

way.

To adjust the wavelength there is no need to return to the method setup. Touch the screen to open

the adjustment screen. Touch the arrow icons to increase or decrease the wavelength. The result

will be adjusted accordingly. Touch the back arrow to return to the measurement screen and the

adjusted results will be displayed on the screen.

5.3 USE A FACTOR MEASUREMENT MODE

The Use a factor measurement mode enables concentration measurements to be performed. In this

measurement mode it is possible to use a known factor to determine the concentration of a solution

based on the measured absorbance:

Concentration = Factor x Absorbance

If the factor isn’t known then the Use Standard(s) measurement mode must be used to calculate

concentration. Touch Use A Factor to enter this measurement mode and begin method setup.

5.3.1 Method Setup

5.3.2 Selecting a Wavelength

Touch Wavelength (nm) to enter the required wavelength. Either type the wavelength required using

the numeric keypad or touch delete to clear the screen and then type in the wavelength. Touch OK to

conﬁrm and save or touch the back arrow to return to the previous screen without saving the entered

wavelength.

5.3.3 Measurement Name

Touch Measurement Name to enter the required name for the sample. This name will be used to

identify the result on any printouts or if the result is saved to USB memory stick. Either type the name

required using the alphanumeric keypad or touch delete to clear the screen and then type in the

name.

Please note the name is restricted to a maximum of 8 characters. Touch OK to conﬁrm and save or

touch the back arrow to return to the previous screen without saving the entered name. The ABC icon

will toggle between alpha and numeric options.

5.3.4 Entering a factor

5.3.5 Units of measure

mg/l

Touch Factor to enter the required factor. The minimum factor which can be entered is

Touch Units to adjust the concentration units. Please note that this is just a text ﬁeld and changing within a unit family e.g. mg/l and g/l will not change the concentration result.

Either type the units required using the alphanumeric keypad or touch delete to clear the screen and

then type in the units. Please note the units are restricted to a maximum of 8 characters. Touch OK

to conﬁrm and save or touch the back arrow to return to the previous screen without saving the

entered units. The 123 icon will toggle between alpha and numeric options.

5.3.6 BLANK CALIBRATION AND SAMPLE MEASUREMENT

A blank calibration must be performed before a sample can be measured. Insert a cuvette containing

the blank solution into the sample chamber. Touch Blank and the instrument will perform a zero

absorbance and 100% transmittance reading.

100.0

Once the blank calibration is complete Read becomes active and the sample can be measured. As

the spectrophotometer measures the entire wavelength range in one scan, if the wavelength is

adjusted before a sample is measured the instrument doesn’t need be calibrated again at the new

wavelength.

Remove the cuvette containing the blank solution and place a cuvette containing the sample to be

measured in the sample holder. Touch Read to measure the sample. Once the measurement is

complete the result will be shown on the screen. Subsequent samples can be measured in the same

way.

To adjust the wavelength there is no need to return to the method setup. Touch the screen to open

the adjustment screen. Touch the arrow icons to increase or decrease the wavelength. The result

will be adjusted accordingly. Touching the back arrow to return to the measurement screen and the

adjusted results will be displayed on the screen.

5.4 USE STANDARD(S) MEASUREMENT MODE

The use standard(s) measurement mode enables sample concentration to be calculated using a

standard curve or a single standard of known concentration. In this mode a number of standard

solutions covering a range of known concentrations are measured at a set wavelength. The

absorbance or % transmittance of these solutions is plotted to create a standard curve with replicate

standards measurements, if required. Once the standard curve has been created a sample of unknown

concentration can be measured and the concentration calculated using the standard curve.

If there is only one standard available this measurement mode will calculate the factor and use that to

calculate the concentration.

Touch Use Standard(s) to enter this measurement mode and begin method setup.

5.4.1 Method Setup

The method parameters enable the creation of a standard curve or a previously created standard curve

can be opened to measure unknown samples against.

The method parameters which can be adjusted are the wavelength, the measurement name, the units

of measure, the number of known standards and the number of replicates. Once these have been

entered touch the forwards arrow to enter the measurement screen. The back arrow will return to the

previous screen and the home icon will return to the home screen. Neither of these options will save

any parameters entered.

5.4.2 Selecting a Wavelength

Touch Wavelength (nm) to enter the required wavelength. Either type the wavelength required using

the numeric keypad or touch delete to clear the screen and then type in the wavelength. Touch OK to

conﬁrm and save or touch the back arrow to return to the previous screen without saving the entered

wavelength.

5.4.3 Measurement Name

Touch Measurement Name to enter the required name for the sample. This name will be used to

identify the result on any printouts or if the result is saved to USB memory stick. Either type the name

required using the alphanumeric keypad or touch delete to clear the screen and then type in the

name.

Please note the name is restricted to a maximum of 8 characters. Touch OK to conﬁrm and save or

touch the back arrow to return to the previous screen without saving the entered name. The ABC icon

will toggle between alpha and numeric options.

5.4.4 Entering Concentration Units

mg/l

Touch Units to adjust the concentration units. Please note that this is just a text ﬁeld and changing within a unit family e.g. mg/l and g/l will not change the concentration result.

Either type the units required using the alphanumeric keypad or touch delete to clear the screen and

then type in the units. Please note the units are restricted to a maximum of 8 characters. Touch OK to

conﬁrm and save or touch the back arrow to return to the previous screen without saving the entered

units. The 123 icon will toggle between alpha and numeric options.

5.4.5 Selecting the Number of Standards and Replicates

The number of standards used can be changed between 1 and 6. If 1 is selected then a calibration

curve will not be created. Touch the number of standards required and touch the back arrow to return

to method setup.

Each standard can have up to 3 replicate measurements performed. If more than 1 replicate is selected

the instrument will take the average absorbance value of the replicates measured to construct the

calibration curve. Touch the number required and touch the back arrow to return to method setup.

5.4.6 Measuring Standards

Before the concentration of each known standard can be measured a blank calibration must be performed. Insert the cuvette containing the blank solution and touch Press to perform blank.

The concentration of each known standard must be entered before it is measured. Touch Set Standard 1 and use the number entry screen to enter the concentration of the standard. Touch OK to conﬁrm and save or press the back arrow to return to the measure standards screen without saving the concentration.

Insert the cuvette containing standard 1 and touch Replicate 1. The instrument will perform a reading and display the absorbance result. If more than one replicate has been selected, touch Replicate 2 to perform the second reading. Repeat if necessary for 3 replicates. Touch the forwards arrow to measure the next standard.

If only 1 standard has been selected then the factor will be displayed. This factor can be used to calculate the concentration of the unknown sample.

If more than 1 standard has been selected each additional standard must be measured in the same way. Touch Set Standard 2 and use the number entry screen to enter the concentration of the standard. Touch OK to conﬁrm and save or press the back arrow to return to the measure standards screen without saving the concentration. Insert the cuvette containing standard 2 and touch Replicate 1.

The instrument will perform a reading and display the absorbance result. Touch the forwards arrow

to measure the next standard or display the calibration curve if only 2 standards have been selected.

Once all the standards have been measured the calibration curve will be displayed. Touch Current Curve Fit to toggle between linear or linear through zero.

Linear: Concentration = Abs x A + B

Linear through zero: Concentration = Abs x A

Where: gradient of the line (A), constant (B) and correlation coefﬁcient (r2).

Touch save to save the standard curve to USB memory stick. If there isn’t a USB memory stick

inserted a warning will be displayed.

Touch the forwards arrow to enter the measurement screen.

Touch Standard Curve Name to enter the required name for the curve. This name will be used to identify the curve on the USB memory stick. Either type the name required using the alphanumeric keypad or touch delete to clear the screen and then type in the name. Please note the name is restricted to a maximum of 8 characters. Touch OK to conﬁrm and remember the entered name or touch the back arrow to return to the previous screen without remembering the entered name. The ABC icon will toggle between alpha and numeric options.

Touch OK to conﬁrm and save or touch the back arrow to return to the previous screen without

saving the entered name. If the curve has been saved the instrument will display conﬁrmation.

Touch the forwards arrow to enter the measurement screen.

5.4.7 Load Standard Curve

It is not necessary to create a new standard curve each time an unknown sample needs to be

measured. Ensure the USB memory stick is inserted into the instrument. Touch Load A Standard

Curve and a list of previously saved standard curves will be displayed. Touch the up or down arrows

to display more results on the screen. The ﬁles are saved in date and time order from most recent to

oldest. Touch the name of the required curve and the standard curve will automatically be loaded.

To return to the previous screen without loading a standard curve touch the back arrow.

Touch the forwards arrow to enter the measurement screen.

To delete a standard curve from the USB memory stick touch delete. Touch the name(s) of the curve

to be deleted. To deselect a curve touch the name of the curve again. Touch OK to conﬁrm or touch

the back arrow to return to the previous screen without loading or deleting any standard curves.

If there are no standard curves saved to the USB memory stick an error message will be displayed.

5.5 BLANK CALIBRATION AND SAMPLE MEASUREMENT

A blank calibration must be performed before a sample can be measured. Insert a cuvette containing

the blank solution into the sample chamber. Touch Blank and the instrument will perform a zero

absorbance and 100% transmittance reading.

100.0

Once the blank calibration is complete Read becomes active and the sample can be measured.

Remove the cuvette containing the blank solution and place a cuvette containing the sample to be

measured in the sample holder. Touch Read to measure the sample. Once the measurement is complete

the result will be shown on the screen. Subsequent samples can be measured in the same way.

Although this spectrophotometer measures the entire wavelength range in one scan if the wavelength

needs to be adjusted a new calibration curve must be created at the new wavelength.

SECTION 6 – Spectrum

The spectrum measurement mode enables measurements of absorbance or % transmittance over a

range of wavelengths to be performed. The absorbance or % transmittance at each wavelength is

plotted graphically. Post measurement tools such as spectral points analysis can be performed. This

operating mode can be used to partially characterise a sample. Touch Spectrum to enter this

measurement mode and begin method setup.

6.1 METHOD SETUP

The method parameters which can be selected are, the measurement name, the measurement mode and the start and end wavelengths. Once these have been entered touch the forwards arrow to enter the measurement screen. The back arrow will return to the previous screen and the home icon will return to the home screen. Neither of these options will save any parameters entered.

800

6.1.1 Measurement Name

Touch Measurement Name to enter the required name for the sample. This name will be used to

identify the result on any printouts or if the result is saved to USB memory stick. Either type the

name required using the alphanumeric keypad or touch delete to clear the screen and then type in

the name. Please note the name is restricted to a maximum of 8 characters. Touch OK to conﬁrm

and save or touch the back arrow to return to the previous screen without saving the entered name.

The ABC icon will toggle between alpha and numeric options.

6.1.2 Selecting Measurement Mode

The measurement mode can be user selected between absorbance and % transmittance. Touch

Measurement Mode to cycle between the two options.

6.1.3 Setting Start and End Wavelengths

This function enables the start and end wavelengths of the spectrum scan to be set. The 7200 has

a spectrum range from 335 to 800nm and measures the entire wavelength range simultaneously.

However the user can select the wavelength range which is displayed. Following the measurement

the start and end wavelengths can be adjusted and the spectrum across the new wavelength range

will be displayed.

Touch Start Wavelength or End Wavelength to enter the required wavelength. Either type the wavelength required using the numeric keypad or touch delete to clear the screen and then type in the wavelength. Touch OK to conﬁrm and save or touch the back arrow to return to the previous screen without saving the entered wavelength. The start wavelength must be smaller than the end wavelength.

Wavelength(s) cannot

be the same

Once the required wavelengths have been entered touch the forwards arrow to move into the measurement screen. If the start and end wavelengths entered are equal, the instrument will display a short warning before returning back to the parameters screen to allow further editing.

If the start wavelength is above the end wavelength a warning will be displayed.

Touch Swap Values to simply swap the start and end wavelengths and continue. Touch Go Back to edit the start and end wavelengths.

Once all the parameters have been measured touch the forward arrow to enter the measurement

screen.

6.2 BLANK CALIBRATION AND SAMPLE MEASUREMENT

A blank calibration must be performed before a sample can be measured. Insert a cuvette containing the blank solution into the sample chamber. Touch Blank and the instrument will before a baseline calibration.

Once the baseline has been completed Read becomes active and the sample can be measured.

Remove the blank cuvette containing the blank solution and place a cuvette containing the sample

to be measured into the sample holder. Touch Read to measure the sample.

As the entire wavelength range is measured simultaneously it is not possible to view a live spectrum

scan as the wavelengths are not measured one at a time. Once the measurement is complete the

spectrum scan will be displayed on the screen and the post measurement tools icon will become

active.

6.3 DATA ANALYSIS

Once a measurement has been completed the post measurement tools icon becomes active. Touch

the post measurement tool icon to open tools screen. This enables access to the zoom function

and the spectral points analysis function.

The zoom function enables areas of interest on the spectrum to be magniﬁed. The spectral points

analysis function enables points to be selected from the scan to analyse absorbance or %

transmittance at selected wavelengths.

6.3.1 Spectrum Zoom

Touch the zoom icon to open the zoom screen. When the spectrum zoom mode is ﬁrst entered, only Zoom in is enabled. Zoom Out, Zoom Left and Zoom Right are only available once the display is showing a zoomed in part of the scan rather than a full scan. To zoom in on a particular section of the scan, either touch the scan on the required part or touch Zoom In to zoom in centrally on the displayed portion of the scan.

The zoom bar at the top of the screen indicates both how far the scan is zoomed in and over which

portion of the original scan the zoomed in portion is from – this is indicated by the white portion of the

bar.

Touch the Zoom In icon to increase magniﬁcation or touch the Zoom Left or Zoom Right arrows

to move the magniﬁcation to a different part of the spectrum. Touch Zoom Out to return to the

original spectrum scan.

Touch the back arrow to return to the previous screen.

6.3.2 Spectral Points Analysis

The spectral points analysis function enables points to be selected from the scan to analyse the absorbance or % transmittance at a selected wavelength. Touch spectral points analysis to open the selection screen.

A solid vertical line will appear on the screen. The position of the line can be moved by touching the arrow icons or by touching the screen. The values at the top of the screen display the wavelength and photometrics result for that point. When the required point is selected touch add to table icon and the selected point will be added to the table. Once the point has been added it will be marked on the spectrum. Up to 50 points can be added to the table.

To delete all the points from the spectral points analysis table touch delete and all previously

selected points will be deleted.

To view the points in the spectral points analysis table touch the table icon. The selected points will be displayed along with the absorbance or % transmittance depending on the measurement mode selected in the method setup. The results will be displayed in numerical order from smallest to largest. To view points not displayed on the screen touch the up or down arrows.

Touch Print to print the spectral analysis points table. An error message will be displayed if an

external printer is not connected or switched on.

Failed to send

data to printer

SECTION 7 – Kinetics

The kinetics measurement mode enables the absorbance or % transmittance of an active molecule

to be measured over a period of time. For example, enzyme analysis of horseradish peroxidase. The

absorbance or % transmittance is measured at regular time intervals at a set wavelength over a period

of time. The results are plotted on a graph to show the change in absorbance or % transmittance over

time. Touch Kinetics to enter this measurement mode and begin method setup.

7.1 METHOD SETUP

The parameters which can be selected are the measurement name, the number of wavelengths, the

measurement mode, run time, lag time and end point concentration units. Once these have been

entered touch the forwards arrow to enter the measurement screen. The back arrow will return to

the previous screen and the home icon will return to the home screen. Neither of these options will

save any parameters entered.

7.1.1 Measurement Name

Touch Measurement Name to enter the required name for the sample. This name will be used to

identify the result on any printouts or if the result is saved to USB memory stick. Either type the name

required using the alphanumeric keypad or touch delete to clear the screen and then type in the

name. Please note the name is restricted to a maximum of 8 characters. Touch OK to conﬁrm and

save or touch the back arrow to return to the previous screen without saving the entered name.

The ABC icon will toggle between alpha and numeric options.

7.1.2 Selecting Number of Wavelengths

Touch Number of Wavelengths to select the required number of wavelengths. Touch 1, 2 or 3 to

select the required number of wavelengths to measure the change in absorbance or % transmittance

over time.

7.1.3 Selecting Measurement Mode

The measurement mode can be user selected between absorbance and % transmittance. Touch

Measurement Mode to cycle between the two options.

7.1.4 Run Time

Touch Run Time (Seconds) to enter the total run time for the kinetics experiment. Either type the

time required using the numeric keypad or touch delete to clear the screen and then type in the time.

Touch OK to conﬁrm and save or touch the back arrow to return to the previous screen without

saving the entered time. The minimum run time is 15 seconds, the maximum run time is 9999

seconds. Due to the size of the instrument memory the instrument can only store 100 measurement

points per wavelength. Depending on the run time entered the instrument will calculate the minimum

measurement interval, subject to a minimum interval of 15 seconds.

7.1.5 Lag time (Seconds)

Seconds

Touch Lag Time (Seconds) to enter the number of seconds that the instrument waits before

commencing measurements after Read has been touched in the measurement screen. Either type

the time required using the numeric keypad or touch delete to clear the screen and then type in the

time. Touch OK to conﬁrm and save or touch the back arrow to return to the previous screen

without saving the entered time. The minimum lag time is 0 seconds, the maximum lag time is 999

seconds.

7.1.6 Selecting Concentration Units

At the end of the kinetics run the concentration of the sample can be calculated using the end point

absorbance value:

Concentration = Factor x absorbance

The units of concentration can be entered here and will appear in the result screen after completion of

the kinetics run.

Touch Units to enter the required concentration units. Either type the units required using the

alphanumeric keypad or touch delete to clear the screen and then type in the units. Please note the

units are restricted to a maximum of 11 characters. Touch OK to conﬁrm and save or touch the back

arrow to return to the previous screen without saving the entered units. The 123 icon will toggle

between alpha and numeric options.

mg/l

7.1.7 Selecting a Wavelength and Factor

The wavelength and factor for each wavelength selected will need to be entered.

Touch Set Wavelength 1 to enter the required wavelength. Either type the wavelength required using

the numeric keypad or touch delete to clear the screen and then type in the wavelength. Touch OK to

conﬁrm and save or touch the back arrow to return to the previous screen without saving the entered

wavelength.

At the end of the kinetics run the concentration of the sample can be calculated using the end point

absorbance value:

Concentration = Factor x absorbance

Touch Endpoint Factor to enter the required factor to calculate the concentration. Either type the

factor required using the numeric keypad or touch delete to clear the screen and then type in the

factor. Touch OK to conﬁrm and save or touch the back arrow to return to the previous screen

without saving the entered factor. If you do not require the concentration to be calculated leave the

factor as a default of 1. The minimum factor which can be entered is -1000, the maximum factor

which can be entered is +1000.

Once the parameters for wavelength 1 have been entered, touch the forwards arrow to enter the

wavelength and end point factor for wavelengths 2 and 3 (if selected), or to enter the measurement

mode.

7.2 BLANK CALIBRATION AND SAMPLE MEASUREMENT

A blank calibration must be performed before a sample can be measured. Insert a cuvette containing

the blank solution into the sample chamber. Touch Blank and the instrument will perform a zero

absorbance and 100% transmittance reading.

Once the blank calibration is complete Read becomes active and the sample can be measured. As the

spectrophotometer measures the entire wavelength range in one scan, if the selected wavelength(s)

are adjusted before a sample is measured the instrument doesn’t need be calibrated again at the new

wavelength(s).

Remove the cuvette containing the blank solution and place a cuvette containing the sample to be

measured in the sample holder. Touch Read to measure the sample.

During the kinetics run the full run time in minutes will be displayed in the bottom right hand corner

of the measurement screen. Touch Run Time to toggle the display between showing the total elapsed

time of the kinetics run or showing the measurement interval count down to the next measurement

being taken.

When the instrument is not taking a measurement the kinetics run can be stopped by touching Stop. Whilst a measurement is being taken, Stop is replaced by the waiting indicator and the button is temporarily inactive to touches.

If a lag time was entered during method set up the instrument will count down the lag time before it begins taking measurements. To stop the lag time count down touch Stop.

Once the measurement is complete the Post Measurement icon becomes active. Subsequent samples can be measured in the same way.

7.3 Post Measurement Analysis

Once the kinetics run is complete it is possible to view the endpoint concentration at each selected wavelength. Touch the Post Measurement icon to display the information. Touch the wavelength to move through the selected wavelengths or touch the backwards

arrow to return to the measurement screen.

SECTION 8 – Glossary of Icons

Icon Description

Back Arrow – return to previous screen

Forwards Arrow – move to next screen

Home – returns to home screen

Instrument Settings – access to instrument settings

Print – to send results to an external printer

Save – to save results to a USB memory stick

Overﬂow – to toggle display between Blank/Read and Save /Print

Up Arrow - to move up or increase

Down Arrow - to move down or decrease

Delete – to delete entry

Space – used when naming results or standard curves

ABC

123

OK - to conﬁrm

ABC – toggles between alpha and numeric keypads

123 – toggles between alpha and numeric keypads

Standard curve selected for deletion

Post measurement analysis tool for spectrum

Icon Description

Spectral zoom

Zoom In

Zoom Out

Zoom to the right (spectrum)

Zoom to the left (spectrum)

Spectral points analysis

Add Spectral point to table

Delete all spectral analysis points or delete selected standard curves

Table – view selected spectral analysis points

Post measurement analysis tool for kinetics

SECTION 9 – Accessories and Spare Parts

9.1 OPTIONAL ACCESSORIES

Part Code Description of Accessory

SMP50/PRINTER External printer

630 204 10 x 10mm path length cuvette holder

637 071 16/24mm test tube holder

630 005 10 to 100mm path length cuvette holder

630 304 Micro-cuvette holder with reduced aperture

019 148 4GB USB memory sticks for external memory

060 084 Pack of 100 disposable cuvettes (visible)

700 000 Dust Cover

9.2 CONNECTING THE ACCESSORIES

There are two types of accessories which can be ﬁtted in the sample chamber – passive (nonpowered)

or active (powered) accessories. The range of passive accessories includes 10 x 10mm single cuvette

holder, adjustable path length (10 to 100 mm) cuvette holder, test tube holder and micro-cuvette

holder. The range of active accessories includes a heated cell. The instrument must be turned off

before any accessories are ﬁtted. There is also an external printer available for instant result print outs.

9.2.1 External Printer

Failed to send

data to printer

Please follow the instructions provided in the SMP50/PRINTER user manual for reﬁlling the

paper and ﬁtting and charging the printer batteries.

9.2.2 Passive Accessories

The external printer can be connected via the USB cable to the USB port on rear of the instrument. To turn the printer on press and hold the power button, until the green lights stop ﬂashing. If the printer isn’t detected when Print is touched or automatic printing is set up an error icon will be shown. This error message will also be shown if the printer fails to print due to no paper.

Unscrew the thumb screw to undo the passive accessory. Lift out the passive accessory. To ﬁt a different passive accessory simply place the accessory in the correct orientation, align the thumb screw and tighten to ﬁx in place.

To replace the passive accessory with an active accessory refer to section 9.2.2.

Thumb screw

Please note that if the adjustable path length cuvette holder is ﬁtted and a cuvette with a path length

greater than 1cm is used, the results need to be manually adjusted according to the Beer-Lambert

law which states that:

A is the absorbance

is the molar absorption coefﬁcient (l mol-1cm-1)

c is the concentration (mol l

l is the path length (cm)

This correction will also need to be applied if test tubes are used. The path length is the diameter

of the test tube.

9.2.3 Active Accessories

Important Notice

The heated cell holder is supplied with a more powerful power supply unit to provide the additional

power required by the heated cell accessory. When installing a heated cell holder, the original

power supply unit supplied with the instrument must be disconnected and replaced with the

power supply unit supplied with the heated cell accessory.

• Power supply unit supplied with instrument: 12vDC, 3.8A

• Power supply unit with heated cell: 12vDC, 5A

A = l c

-1

)

Failure to use the appropriate power supply unit when using the heated cell may cause damage

to the power supply unit which will not be covered by the manufacturer’s warranty.

Ensure the instrument is switched off, disconnected from the mains power and the power supply has been changed over as described above. Remove the passive accessory as described in Section 9.2.1. Place the heated cell accessory into the sample chamber. Connect the plug to the socket in the back right hand side of the chamber. The plug and socket will lock

Socket for heated cell accessory

For instructions on how to operate the heated cell accessory refer to section 4.4.

To remove the heated cell accessory ensure that the instrument is switched off and unplugged

from the mains power. Unscrew the thumb screw and disconnect the accessory power lead by

holding down the top clip on the connector and pulling the connector backwards.

If the heated cell accessory is removed it is not necessary to change the power supply unit back

to the less powerful power supply unit originally supplied with the spectrophotometer.

together as the locking tag on the top of the plug ‘clicks’ into place.

9.3 SPARES

The spare parts for the 7200 spectrophotometer are listed below:

Part Code Description

012 050 Tungsten Halogen lamp module

630 204 10x10mm path length cuvette holder

M7980 12V 3.8A power supply unit for spectrophotometer

607 167 12V 5A power supply unit for the heated cell accessory

M7817UK UK Power lead

M7817X6 EU Power lead

US7817X1 US Power lead

037 702 Paper roll for printer

SECTION 10 – Maintenance and Service

10.1 ROUTINE MAINTENANCE AND CLEANING

Ensure the external surfaces of the unit are clean and free from dust. The sample area should

always be kept clean and any accidental spillage should be wiped away immediately. To give added

protection when not in use, the unit should be disconnected from the mains supply and covered

with the optional dust cover.

In the event of accidental spillage on or within the instruments measurement compartment,

immediately switch off the equipment and disconnect from the mains supply. Dry the unit with a

cloth and leave to dry for an appropriate period before commencing use.

If the instrument needs to be cleaned ensure the equipment is switched off and disconnected from

the mains supply before cleaning. Wipe down the unit with a soft damp cloth and a mild detergent

solution. Do not use bleach or abrasives. Do not allow cleaning liquids to ingress inside the

equipment. Never immerse the unit, cables or plugs in water or any other liquids. Allow any wet

surfaces to dry before re-connecting to the mains supply and commencing use.

Servicing should only be carried out by qualiﬁed personnel.

Service personnel should be aware that the lamp, its holder and surrounding enclosure will be

extremely hot (above 200°C) during use and for a period of time after use. Before starting any

maintenance or service, switch the instrument OFF, remove from the mains supply and allow to

cool for at least 20 minutes. Wear protective gloves to prevent injury when replacing the lamp or

working within the vicinity of the lamp.

Do not work within the instrument while the lamp is ON as exposure to the high intensity light can

cause injury to your eyes.

10.2 LAMP REPLACEMENT

10.2.1 Halogen Lamp Module Replacement

Before replacing the lamp disconnect the unit from the mains supply and ensure the lamp is cool

before handling. The instrument should be switched off for at least 20 minutes. Wear ‘lint free’

protective gloves when replacing the lamp to avoid injury from burns and to prevent marking the

lamp with ﬁnger prints.

Access to the tungsten halogen lamp can be gained via the lamp access panel located on the

underside of the instrument (refer to section 2.5).

1. Remove the screws holding the lamp access panel in place.

2. Remove the lamp cover.

3. Remove the two ﬁxing screws and washers which retain the lamp assembly (ﬁg 1).

4. Detach the electrical connector (ﬁg 2).

5. Grab the two tabs on the plastic lamp assembly and remove the whole lamp assembly,

this may need to be rotated slightly to allow it to be removed (ﬁg 3).

6. Carefully remove the replacement lamp from the packaging and remove the protective

cover from the lamp assembly (ﬁg 4). Ensure that the glass portion of the lamp is not

touched as ﬁnger marks will damage the lamp resulting in a reduced performance. If

accidental damage occurs the surface of the lamp may be cleaned using propan-2-ol.

7. Insert the new lamp assembly into the same position as the one just removed. This may

need to be rotated slightly to be fully inserted. Ensure that the ﬁxing holes in the plastic

lamp assembly line up with the ﬁxing holes in the unit. If the holes do not line up then the

lamp assembly must be rotated 180 degrees. The lamp assembly must be pulled back

out to do this.

8. Fix the two screws and washers removed earlier back in position to fully secure the lamp

assembly. It must be fully pulled against the black aluminium ﬁxing plate.

9. Re couple the electrical connector.

10. Replace the lamp cover ensuring no wires are trapped and ﬁx in position with the two

screws.

11. Place the unit back in its correct orientation and ensure there are no samples or cuvettes

in the holder and that the 10x10 cuvette holder is ﬁtted.

12. Reconnect the power supply, turn the unit on and allow time for the unit to self-calibrate

with the new lamp.

Fig. 1

Fig. 3

Fig. 2

Fig. 4

10.3 FIRMWARE UPDATE PROCEDURE

The 7200 spectrophotometer allows users to update the instrument’s ﬁrmware using a USB ﬂash

drive using the following procedure:

1. Download the latest version of the instrument’s ﬁrmware from the Jenway website at

www.jenway.com/Software.asp and copy the ﬁle to a USB ﬂash drive.

2. Switch on the power to the instrument.

3. Insert the USB ﬂash drive into the USB memory stick slot of the instrument.

4. Refer to section 4.7 to complete the update procedure.

10.4 SERVICE

Our dedicated service team are on hand to help in the unlikely event that your Jenway equipment

develops a fault. Please contact them by one of the following means with a clear description of the

problem:

E-mail: service@bibby-scientiﬁc.com

Tel: +44 (0) 1785 810475

Fax: +44 (0) 1785 810471

On occasion it may be necessary for your equipment to be sent back to our Service Department for

repair. In this case please contact the Service Department for a reference number which you should

include with your faulty equipment. Please also ensure you include a clear description of the fault

and a completed copy of our Decontamination Certiﬁcate. This is available as a downloadable pdf

ﬁle at www.jenway.com, or contact us and we will be happy to fax you a copy. Please clearly mark

the package for the attention of the Service Department and post to the following address:

Bibby Scientiﬁc Ltd

Beacon Road

Stone

Staffordshire

ST15 0SA

United Kingdom

All replacement parts are guaranteed for 1 year and where ever possible, returned equipment is

turned around in 10 working days.

SECTION 11 – Troubleshooting

11.1 ERROR CODES

The table below shows the error codes and solutions:

No USB stick found

Ensure a USB memory stick is inserted into the front USB port on the instrument.

Couldn’t ﬁnd any standard curves

There are no curve ﬁles stored on the USB stick. Ensure a USB memory stick is inserted into the front USB port on the instrument when the curve is saved.

Couldn’t ﬁnd any standard

curves

An error occurred deleting one

or more ﬁles

Power up

calibration failure

An error occurred deleting one or more ﬁles

Each selected curve is individually deleted – if there is a problem deleting one or more curves, this is displayed.

Power up calibration failure

This is displayed if there is a failure when attempting a power up calibration. Touch retry to try the calibration again. If the problem persists, contact technical support or service.

retry

Remove the cuvette

Remove the

cuvette

retry

A communications timeout

has occurred

Remove the cuvette from the sample chamber and touch retry to try the calibration again. If the problem persists, contact technical support or service.

A communications timeout has occurred

If the problem persists, contact technical support or service.

Wavelength(s) cannot be the same

Wavelength(s) cannot

be the same

Start wavelength (600nm) is

above end wavelength

(500nm)

Swap Values

Go Back

The start and end wavelengths of the spectrum scan cannot be the same value. Ensure that the start wavelength is lower than the end wavelength.

Start wavelength is above end wavelength

The start wavelength of the spectrum scan cannot be above the end wavelength. Ensure that the start wavelength is lower than the end wavelength.

Standards can’t be the same

Standard 1 = 5.000 Abs

When creating a standards curve the standards used must have different concentrations.

Unable to calculate a curve

Failed to send

data to printer

Unable to calculate curve

This can happen if the line of best ﬁt is completely vertical. If either m or c calculate as inﬁnity this error will be displayed. Ensure the standard concentration values have been inputted correct and the standards have been measured in the correct order.

Failed to send data to printer

Ensure an external printer is attached using the USB port on the rear of the instrument, that the printer is turned on and has paper in it.

Unable to start

bootloader

retry

Unable to start

main application

retry

Unable to start bootloader

If the problem persists, contact technical support or service.

Unable to start main application

If the problem persists, contact technical support or service.

Unable to

peform read

Unable to perform read

If the problem persists, contact technical support or service.

Current Version

1.1.0/01.03 (14/07/16)

Version on USB

No update found on stick.

Load Software

One or more method

parameters was out of range.

File has not been opened

No update found on stick.

The USB memory stick doesn’t contain a valid software update ﬁle. Ensure that the update has been saved to the USB memory stick.

One or more method parameters was out of range. File has not been opened

When reloading a curve from USB stick, all the parameters of the curve are checked to see that they are valid. If some of the parameters are out of range the ﬁle will not be loaded. Create a new standard curve.

11.2 TROUBLESHOOTING GUIDE

Issue Solution

Unable to achieve zero absorbance or Ensure that there is not a sample in the sample

100% transmittance when calibrating chamber.

Ensure the lamp is working – if the lamp has failed

please request service assistance.

Unable to achieve a reading when Ensure the correct cuvette is being used so that light

measuring a sample isn’t being absorbed by the cuvette.

Ensure the sample isn’t too dense that light is not

transmitted through the sample.

Ensure the lamp is working. if the lamp has failed

please request service assistance.

Unable to add additional points to the The table can hold 50 points.

spectral points analysis table

Unable to save results to the USB Ensure the USB stick is inserted into the front of the

memory stick unit.

Ensure the USB memory stick has free capacity.

Ensure there is a result displayed on the screen.

Fails start up calibration Ensure that a 12V power supply is connected to the

instrument. A smaller voltage power supply will be

able to power the instrument but it will not provide

enough power to drive the lamp.

11.3 TECHNICAL SUPPORT

Jenway have a dedicated Technical Support team made up of experienced scientists who are on hand

to help with any applications advice and questions you may have about our products and how to use

them. If you require any technical or application assistance please contact the team at:

E-mail: techsupport@bibby-scientiﬁc.com

Phone: +44 (0)1785 810433

Fax: +44 (0)1785 810405

SECTION 12 – Declaration of Conformity

SECTION 12 – DECLARATION OF CONFORMITY

Bibby Scientific Ltd

Beacon Road Stone Staffordshire ST15 0SA United Kingdom Tel: +44 (0)1785 812121 e-mail: info@bibby-scientific.com www.bibby-scientific.com

Jenway 7200 Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual