Jenway 6300 Service manual

Jenway 6300

Spectrophotometer Service Manual

Main Index

Section 1 Introduction

Section 2 Quick Reference

Section 3 System Description

Section 4 Optical Description

Section 5 Electronic Description

Section 6 Software and Operation

Section 7 Diagnostics

Section 8 Maintenance

Section 9 Circuit Diagrams

Section 10 Assembly Diagrams

Section 11 Spare Parts List

Jenway 6300 Ser Man

Section 1

Introduction

1.0 Index to Sections

1.1 About This Manual

1.2 Using This Manual

1.3 Warnings & Safe Practice

1.4 Standards & Certification

1.5 Ordering Spares

1.6 Returning Items

1.7 Contacting Jenway Limited

Jenway 6300 Ser Man

Introduction.

1.0 Index to Sections

Section 1 – Introduction

1.0 Index to Sections

1.1

1.2

1.3

1.4

1.5

1.6

1.7

About This Manual Using This Manual Warnings & Safe Practice Standards & Certification Ordering Spares Returning Items Contacting Jenway Limited

Section 2 - Quick Reference

2.0 About ‘Quick Reference’

2.1

2.2

2.3

2.4

2.5

2.6

2.7 Test Solutions

Specification Main Sub-Assemblies Power Supply Voltages Signal Levels Error Codes Special Key Functions

Section 3 - System Description

3.1

3.2

3.3

3.4

Background Sub-Assemblies Accessories Outputs

Section 4 - Optical Description

4.1

4.2

4.3

4.4

Jenway 6300 Ser Man

Light source Grating Shutter and Filter Signal Detector

Section 5 - Electronic Description

5.1

5.2

5.3

Power Supplies Detector Circuit Microprocessor and Display

Section 6 - Software and Operation

6.0 Warning

6.1

6.2

6.3

Start Up Routine Photometrics Concentration

Section 7 – Diagnostics

7.1

7.2

7.3

7.4

Diagnostics Mode Shutter and Filter Control Lamp Control Zero Order Calibration.

Section 8 – Maintenance

8.1

8.2

8.3

8.4

8.5

8.6

8.7

8.8

Routine Maintenance Dismantling Optical Alignment Energy Levels Wavelength Calibration A to D Calibration D to A Calibration Performance Verification

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Section 9 - Circuit Diagrams

9.1 Supply Schematic 630 504

9.2 Power Supply Layout 630 504

9.3 Detector PCB Schematic 630 506

9.4 Detector PCB Layout 630 506

9.5 Microprocessor PCB Schematic 630 013

9.6 Microprocessor PCB Layout 630 013

Section 10 - Assembly Diagrams

10.1 6300 Final Assembly 630 503

10.2 6300 Lower Case Assembly 630 510

10.3 6300 Top Case Assembly 630 010

10.4 6300 Optics Assembly 630 508

10.5 6300 Rear Panel Assembly 630 012

Section 11 – Spare Parts List

11.01 Packed Instrument

11.02 Top Case Assembly

11.03 Microprocessor/display PCB

11.04 Lower Case Assembly

11.05

Jenway 6300 Ser Man

1.1 About This Manual

This manual covers the service, maintenance, calibration and repair of the Jenway Ltd model 6300 Spectrophotometer. (From serial number 5000 upwards, for lower n umbers please refer to 6300 Mk1 Manual) This manual must be used in conjunction with the Instruction Manual for this model, as many of the routine maintenance procedures detailed in the Instruction Manual are not repeated in this Service Manual.

1.2 Using This Manual

This manual is only for the use of Engineers and Technicians who have successfully completed a Jenway Ltd approved Service Training course on the Model 6300 Spectrophotometer.

Updates to thi s manual will be c irculated through the Jenway Ltd TSI (Technical Service Information) systems and to other registered users of this manual. Please complete the form at the rear of this manual to register your copy for future updates.

In practice Section 2 - Quick Reference and Section 8 – Maintenance, with the Diagrams in Section 9 and 10, wi ll be most frequently used, however it is good practice to read the complete manual initia lly and review it again periodically.

To find the information required refer to the Main Index or Index to Sections to ide ntify the relevant Section/page number required.

1.3 Warnings & Safe Practice

Always disconnect the mains supply when any covers are removed as there are voltages present inside the unit that pose the risk of electric shock at levels that are hazardous to life!

Do not look directly at the light source or allow the light beam to fall directly on the eyes, switch off or dim the lam p (as described in

Jenway 6300 Ser Man

the procedures) whenever possible and wear eye protection at ALL times.

The lamp gets very hot when in use, alwa ys allow time for it to cool down before removal. Always wear cotton gloves when removing a faulty lamp and replacing with a new one.

Finger marks, dust and condensation can quickly destroy sensitive and expensive optical components, always w ear cotton gloves when the optical b e nch is uncovered and handle any components by their edges only. Never touch optical surface s. Do not remove optical covers unless the unit is in a clean, dust and condensation free environment.

Many of the reagents, solutions and standards used for maintenance and calibration are corrosive or hazardous, ensure all precautions supplied with them are followed, where there is any doubt request a MSDS (Material Safety Data Sheet) from the supplier.

This instrum e nt can be used for analy sing a broad range of samples, do not handle them unless you are qualified to do so. Ensure that the instrument has been correctly decontaminated before working on it, specifically in areas where the instrument may have been used for clinical, biological, corrosive or radioactive samples.

1.4 Standards & Certification

No adjustments should be made to this instrument unless the test and measurement equipment, signal source or filters to be use d have a current calibration certificate that is traceable to national or international standards and that it is known that this test equipment is currently performing to the cert ified standard s. All solution s and reagents should be fresh and within any stated shelf life with a certificate of analysis.

1.5 Ordering Spares

When ordering spare parts as detailed in this manual please quote the Part Number and Description. These items should be ordered from the original supplier of the equipment or your local Jenway Limited Distributor.

Jenway 6300 Ser Man

1.6 Returning Items

Should it be necessary to return any item for any reason then this should be done through the original supplier of the equipment or your local Jenway Limited Distributor.

1.7 Contacting Jenway Limited

Before contacting Jenway Lim ited please check our we b pages for any information or updates that may be helpful to you. www.jenway.com

Emails should be sent to sales@jenway.com

Fax: +44 1371 821083

Phone: +44 1371 820122

Please note no items can be returne d to (or will be accepted by) Jenway Limited without a Returns Authorisation number (RA number) and a completed Safety Clearance and Decontamination certificate.

Jenway 6300 Ser Man

Section 2

Quick Reference

2.0 About ‘Quick Reference’

2.1 Specification

2.2 Main Sub-Assemblies

2.3 Power Supply Voltages

2.4 Signal Levels

2.5 Error Codes

2.6 Special Key Functions

2.7 Test Solutions

Jenway 6300 Ser Man

2.0 About ‘Quick Reference’

This section c ontains a selectio n of the key information that is often forgotten or difficult to find when required. Use Quick Reference as a memory jogger, but for more information check out the references to the main sections on each point.

2.1 Specification

Also see Section 1.2 of the Instruction Manual.

Wavelength R a nge 320nm to 1000nm Wavelength Resolution 1nm Wavelength Acc uracy +/-2nm Spectral Bandwidth 8.0nm Light Source Tungsten Halogen Optics Single Beam Transmittance Range; 0 to 199.9%

Resolution; 0.1%

Absorbance Range; -0.300A to 1.999A

Resolution; 0.001A

Concentration Range; -300 to 1999

Resolution; 0.1, 1

Units; ppm, mgl Factor 0 to 999.9 / 1000 to 9999 Photometric Accuracy +/-1% Photometric Noise Less than 1% Stray Light Less than 0.5%T Photometric Stab ility Better than 1% per Hour (after warm up) Readout Custom LCD Outputs Analogue (0 to 1999mV) & RS232 Serial Supply Voltages 115/230 V a.c. Power Less than 50W Dimensions 365 (w) x 272 (d) x 160 (h) mm Weight 6Kg

-1

, gl-1, M, %, blank.

Jenway 6300 Ser Man

2.2 Main Sub-Assemblies

Also see Section 11 – Sp are Parts

630 010 Top Case Assembly – includes the following…

630 025 Keypad 630 013 Display PCB

630 510 Lower case Assembly – includes the following…

630 508 Optics Assembly 630 504 Power Supply PCB 630 506 Detector PCB 060 311 Cooling Fan 010 040 Torroidal Transformer

630 508 Optics Assembly – include s the following…

012 075 Tungsten Halogen lamp 032 005 12V Solenoid 630 516 IR Filter

630 012 Rear Panel Assembly – includes the following…

016 021 2A Fuse 20 x 5mm 017 050 Mains Switch 009 123 Mains Input Socket

Jenway 6300 Ser Man

2.3 Power Supply Voltages

Also see Section 9 - Circuit Diagrams

Before commencing more complex fault finding it is important to check all the internally generated supply voltage s are correct. The following list is a useful guide to help quickly check these are functioning correctly. Not all the points where these voltages can be measured are given and where the voltage is stated as unregulated variations may occur. In general regulated supplies should vary by no more than +/-5% from their nominal value.

Tungsten Lamp Supply

, 12V dc regulated and set by VR1, measure at SK9 pin 5 with respect to SK9 pin 6 on the power supply PCB and at the terminals on the lamp base with the lamp fitted.

Solenoid and Fan Supplies

, 12V dc regulated and pre-set, measure at SK1 pin 1 with respect to SK1 pin 2 on the power supply PCB and on th e solenoid and fa n terminals.

Digital Supply

, 5V dc regulated and pre-set, measure at SK5 pin 1 with respect to SK5 pin 2 or on SK1 pin 1 with respect to SK1 pin 2 on the Display PCB.

Stepper Motor Drive

, 30V dc unregulated, also acts as unregulated supply for lam p, solenoid and fan supplies, measure between Star1 and Star 2 on power supply PCB.

DAC Supply,

+/- 10V dc supplies. +10V dc generated on the RS232 Interface, IC101, on the Display PCB, measure between pins 2 (positive) and 15 (0V) on IC101 on the Display PCB.

-10V dc generated on the RS232 Interface, IC101, on the Display PCB, measure between pins 8 (negative) and 15 (0V) on IC 101 on the Display PCB.

Jenway 6300 Ser Man

2.4 Signal Levels

Also see Section 7.2 - Shutter and Filter and 8.3 – Energy Levels

All analogue signal processing is dealt with on the Detector PCB. The output from the detector is shown in the Diagnostics mode as a Voltage, in mV. For more information see Section 5.2 - Detector Circuit and Sect ion 7.1 – The Dia gnostics Mode .

This voltage display can be used to check lamp energy (ageing), the correct functioning of the IR Stray Light filter as well as the Dark Shutter.

320nm Energy

, In the Diagnostics Mode (see Section 7.1 Diagnostics Mode) set the wavelength to 320nm, close the Dark Shutter by pressing the right arrow key . Allow the mV reading to stabilise and rec ord the stable val ue.

Open the dark shutter by pressing the right arrow key and the mV reading must increase by more than 4mV from that recorded above.

If not the lamp sh ould be changed, if it still has not improved then the condition and alignment of the optical components should be checked.

Dark Current

, Set wavelength to 320nm, Dark Shutter closed, IR

stray light filter closed, Voltage Display should be zero +/- 6mV.

If not and no light leaks are obvious (damaged seals around sample chamber, lid not closing fully , damaged or poorly fitted casework etc) then the detector or detector PCB may be faulty.

720nm Output

stray light filter open, Voltage Display must not be greater than 3600mV.

If greater than 3600mV check the lamp, lamp supply voltage, other power supply levels and detector PCB.

Jenway 6300 Ser Man

, Set wavelength to 720nm, Dark Shutter open, IR

2.5 Error Codes

See also Section 3.1 and 3.2 of the Instruction Manual

A number of Error Codes are generated that relate to various fault conditions, these are detailed below with a brief description of some of the most common causes for these errors.

Err 1

, Dark level too high during a functional calibration. In normal operation the dark shutter closes during an operator instigated calibration sequence to ensure that the detector output is below a threshold level. The calibration is aborted and Err 1 indicated if the detector ou t put is abo ve this t hre shol d lev el. The most likely cause is that the sample chamber door has been left open or was opened during the calibration sequence, it may also occur due to a faulty detector PCB.

Err 2

, Light level too low during a functional calibration. This may be caused by the sample/calibrant being too op tically dense (dark), it may also be caused by lamp failure as well as the use of plastic or glass cuvettes at wavelengths where these materials will absorb a high proportion of the light energy.

Err 3

, Standard out of range in a concentration measurement. This may be because the standard is too optically dense at the wavelength selected or it may be too similar to the blank. Dillution of the sample may be applicable for the former or the wavelength for the later method may require adjustment.

Err 4

, No zero order (white) light found during wavelength calibration in start up routine. This error is given at the end of the wavelength calibration routine, indicating that the test was partly successful (see Section 6.1 Start Up Routine). Hence this error is normally caused by low energy levels due to lamp ageing or contamination/deterioration of other optical components. Also ensure that a sample or cuvette has not been left in th e sam ple chamber during the start up tests and that there is no splashing or spillage on the exit and detector lenses.

Err 5

, No zero order (white) light found during wavelength calibration in start up routine. This error is given during the wavelength calibration routine, indicating that even the initial threshold level was not achieved. Possible causes are; incorrect

Jenway 6300 Ser Man

lamp fitted, cuvette/sample or other obstruction in the light path through the sample chamber as well as a possible optical component failure or misalignment.

Err 6

, No dark level found in start up routine. Leavi ng the sample chamber open during the start up routine or opening it once the procedure has started can cause this error. It can a lso indicate the failure of the dark shutter solenoid, the solenoid drive or the associated control circuits.

Err 7

, Grating position sensor not detected. This error occurs during the wavelength calibration procedure in the start up routine and is generated at the beginning of this procedure. The grating is driven in a clockwise direction until the attached vane breaks the path of an opto-coupler. This is effectively the ‘end-stop’ and the point from which auto-calibra tion will always start. Failure to detect this point may be due to a faulty opto-coupler, wavelength drive from the power supply PCB, a faulty motor or mechanical coupling.

2.6 Special Key Functions

There are a number of special key functions for use by trained engineers, do not use them unless you are fully conversant with all the procedures these invoke.

Power On Reset

. Hold the <Enter> key depressed while turning on the power. This clears the operator set parameters held in nonvolatile memory and is useful in correcting many software conflicts. As th is procedure by passes the start up routine the instrument must be re-booted before use.

Analogue Output Calibration

. Hold the <up arrow> key depressed while turning on the power. This enables fine calibratio n of the analogue output against internally generated reference voltages. See Section 8.6.

View Start Up Routine.

Hold the <Cal> key depressed while turning on the power. This enables the detector output and grating position to be monitore d on the dis pla y during t he Start Up Routine.

Jenway 6300 Ser Man

A to D Calibration.

Hold the <Print> key depressed while turning on the power. This enables the A to D converter on the detector PCB to be calibrated against an external precision voltage source. See Section 8.5.

Diagnostics Mode.

Hold the <right arrow> key depressed while turning on the power. The main display shows the signal from the detector in mV and the resolution of the wavelength display is increased to 0.5nm. See Section 7. 1. Further special key functions are invoked in the diagnostics mode as follows;

Further presses of this key will toggle the dark

shutter open and closed. See Section 7.2.

Alternate presses of this key will reduce the lamp

voltage to 5V (dim) and then return it to 12V. See Section 7.3.

<Cal>

This key is used to set the wavelength display to 0.0 with the grating in a position where white light (zero order) is reflected on to the detector. A second press of this key is required to confirm the setting before resuming the normal diagnostic functions. See Section 7.4.

<print>

Alternate presses of this key will toggle the Infra-red filter

in and out of the light path. See Secti on 7.2.

2.7 Test Solutions

1. Holmium Perchlorate – 5% w/v solution of Holmium Oxide in 1.4N Perchloric acid, this will give absorbance maxima at 361.4, 416.1,

451.1, 485.3, 536.5 and 640.5nm.

2. Potassium Dichromate – 100.0mg/l in 0.005M Sulphuric Acid (use the Sulphuric Acid as the blank). This will give an Absorbance value of

1.071 at 350nm . Potassium Dichromate – 50.0mg/l in 0.005M Sulphuric Acid (use the Sulphuric Acid as the blank). This will give an Absorbance value of

0.536 at 350nm .

3. Sodium Nitrate – 50g/l in de io n ise d wate r, sho uld gi ve les s tha n 0.1% Transmittance at 340nm.

All these solutions are hazardous and the manufacturer/suppliers safety precautions should be carefully followed at all times in preparation, use and storage.

Jenway 6300 Ser Man

System Description

3.1 Background

3.2 Sub-Assemblies

Section 3

3.3 Accessories

3.4 Outputs

Jenway 6300 Ser Man

3.1 Background

The model 6300 is a single beam, visible spectrophotometer with Absorbance, Transmission and Concentration measurement modes. It is a direct replacement for the earlier model 6100.

This manual covers the service, maintenance an d repair of all units with a serial number greater than 5000. For the service, maintenance and repair of units with serial numbers less than 5000 please refer to the 6300 Mark 1 Service Manual.

3.2 Sub-Assemblies

The model 6300 spectrophotometer can easily be broken down into sub-assemblies for the purposes of repair or repla cement. All the PCBs are easily removed, see Section 8.2 – Dismantling. The monochromator is also a replaceable sub-assembly. There is a range of sampling accessories that can be easily fitted and removed from the sample chamber.

See Section 2.2 for details of the main sub-assemblies and Section 11 for details of other spare parts. The foll owi ng para gra p h lists th e sampling accessories available.

3.3 Accessories

The following sampling accessories are a vailable, where necessary additional service information for these accessories is available on request. The development of other sampling acce ss or ies is continuous, please check current brochures or to-date information.

632 001 External Sipper Pump (230V) 632 031 External Sipper Pump (115V) 634 001 4 Position Cell Changer 630 020 Test Tube Holder (13mm diameter) 630 021 Test Tube Holder (25mm diameter) 630 022 Test tube Holder (16mm diameter) 630 005 20 to100mm Single Cell Holder 648 001 Water Heated Single Cuvette Holder

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for up-

Jenway 6300 Ser Man

Other accessories include:

543 001 External 40 Column Printer 542 009 Interface Cable Kit 630 028 Dust Cover 037 201 Water/refrigerant Circulator for use with 648 001

3.4 Outputs

The 6300 has both analogue and RS232 outputs.

Details of the level of the analogue output for the different ranges that may be selected on the instruments is given in Section 6.3 of the Instruction Manual.

Pin configuration for the RS232 socket is given in Section 6.2 of the Instruction Manual. Section 6.1 of the Instruction Manual gives details of the various ASCII codes that may be transmitted to the 6300 to enable complete remote control from a terminal or PC.

Jenway 6300 Ser Man

Optical Description

4.1 Light Source

4.2 Grating

4.3 Shutter and Filter

Section 4

4.4 Signal Detector

Jenway 6300 Ser Man

4.1 Light Source

The 6300 uses a single Tungsten Halogen lamp to cover the full wavelength range from 320 to 1000nm. This is a pre-focussed lamp selected for the accurate position of the filament, making realignment after replacement unnecessary.

The use of lamps other than those supplied by Jenway Ltd (part number 012 075) will cause error codes to be displaye d and result in erroneous results. See Section 4. 2 of the Instructi on Manual.

4.2 Grating

From the lamp the light passes through a slit and lens assembly then onto a flat holographic grating with 1200 lin e s per millimetre.

The grating is directly coupled to the stepper motor, w hich is under microprocessor control. An opto-coupler mounted just behind the grating acts as an ‘end stop’ when the sensor vane connected to the grating mount blocks the l ight path acros s it.

4.3 Shutter and Filter

The diffracted spectrum then passes through a further slit a nd lens arrangement after which the light beam can be blocked by a shutter switched in an d out by an electronically operated solenoid.

Before passing into the sample chamber the monochromatic light passes through an infra-red filter when wavelengths below 360nm are selected. Similar to the shutter this filter is switched in or out by an electronically operated solenoid under microprocessor control. .

4.4 Signal Detector

An S1133 photo-diode is used as the detector on the 6300, it is mounted directly onto the dete ctor PCB, behind the lens block

The detector PCB carries out all of the analogue signal processing, including the A to D conversion, and has its individual calibration data stored in a n on-board E Section 5.2 – Detector Circu it.

PROM. For more information see

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