Eppendorf twin.tec PCR Plates User Manual

Instructions for useEppendorf twin.tec® PCR Platesseeon p.Fig.Tab.p.

English (EN)Instructions for use

Instructions for use

Eppendorf twin.tec
English (EN)

®

PCR Plates

Before using the consumables for the first time, read these instructions for use and the
operating manual of the device that you use the consumables with.

1 Product description

The Eppendorf twin.tec PCR Plate is a single-use polycarbonate/polypropylene plate
allowing trained specialists to perform PCR and cycle sequencing experiments. It is used
in training, routine and research laboratories in the bioscientific, industrial and chemical
fields. Evaporation is prevented with the aid of a variety of sealing systems.

The Eppendorf twin.tec real-time PCR Plates differ from Eppendorf twin.tec PCR Plates by
their white wells. They are intended for real-time PCR.

The Eppendorf twin.tec microbiology PCR Plates have additional sterility features. Besides
the quality criterion PCR clean, these plates are tested to be free of bacterial DNA, ATP
and pyrogen. Gassing with ethylen oxide is used as sterilization method.

2 Safety

WARNING! Damage to health from toxic, radioactive or aggressive chemicals as
well as infectious liquids and pathogenic germs.

 Observe the national regulations for handling these substances, the biological

security level of your laboratory, the material safety data sheets and the
manufacturer's application notes.

 Wear personal protective equipment.
 For full instructions regarding the handling of germs or biological material of

risk group II or higher, please refer to the "Laboratory Biosafety Manual"
(Source: World Health Organization, current edition of the Laboratory Biosafety
Manual).

WARNING! Risk to health from escaping substances.

 Only use optically perfect and undamaged twin.tec PCR Plates.
 Observe the maximum filling volume. Liquid may splash out when the selected

sealing system is removed.

 Seal the twin.tec PCR Plates prior to centrifugation. Observe the instructions in

the device operating manual.

 Store and transport the twin.tec PCR Plates in a way that prevents material from

escaping from the twin.tec PCR Plates.

 Do not use the twin.tec PCR Plates as cryo tubes.

Instructions for use

2

Eppendorf twin.tec
English (EN)

®

PCR Plates

 Take appropriate measures to avoid contaminations after opening the bag containing

PCR clean consumables.

 Reagents, consumables contaminated with reagents and materials used for cleaning

and disinfecting should be disposed of in accordance with laboratory regulations.

3 Application

For PCR instructions please refer to reagent manufacturers' work specifications or lab
protocols.

3.1 Filling

Maximum filling volume:

• twin.tec PCR Plate 96, semi-skirted and unskirted: 250 µL

• twin.tec PCR Plate 96, skirted and unskirted, low profile: 150 µL

• twin.tec PCR Plate 384: 45 µL

• twin.tec real-time PCR Plate 384: 40 µL

 Fill the twin.tec PCR Plates directly or place them in an appropriate stand for filling.

3.2 Sealing

 You can use cap strips, adhesive PCR foils and films or heat sealing foils and films to

seal the twin.tec PCR Plates.
We recommend heat sealing with Eppendorf Heat Sealing Foil or Eppendorf Heat
Sealing Film as the ideal protection against evaporation.

Perfect sealing of the PCR Plates allows an oil-free PCR reaction in a minimum volume of
up to 10 µL (96-well PCR Plates) or 5 µL (384-well PCR Plate).

3.3 Real-time PCR

An application for plates with white wells is to enhance the signal recovery and thus the
detection limit in real-time PCR applications. This is the case with a dye which has a low
concentration or low fluorescence intensity. Generally plates with white, signal amplifying
wells can cause irradiation - depending on the device - if the concentration of the dye is
too high. In this case, you should reduce the concentration of the probe or of the dye.