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Instructions for useEppendorf twin.tec® PCR Platesseeon p.Fig.Tab.p.
English (EN)Instructions for use
Instructions for use
Eppendorf twin.tec
English (EN)
®
PCR Plates
Before using the consumables for the first time, read these instructions for use and the
operating manual of the device that you use the consumables with.
1 Product description
The Eppendorf twin.tec PCR Plate is a single-use polycarbonate/polypropylene plate
allowing trained specialists to perform PCR and cycle sequencing experiments. It is used
in training, routine and research laboratories in the bioscientific, industrial and chemical
fields. Evaporation is prevented with the aid of a variety of sealing systems.
The Eppendorf twin.tec real-time PCR Plates differ from Eppendorf twin.tec PCR Plates by
their white wells. They are intended for real-time PCR.
The Eppendorf twin.tec microbiology PCR Plates have additional sterility features. Besides
the quality criterion PCR clean, these plates are tested to be free of bacterial DNA, ATP
and pyrogen. Gassing with ethylen oxide is used as sterilization method.
2 Safety
WARNING! Damage to health from toxic, radioactive or aggressive chemicals as
well as infectious liquids and pathogenic germs.
Observe the national regulations for handling these substances, the biological
security level of your laboratory, the material safety data sheets and the
manufacturer's application notes.
Wear personal protective equipment.
For full instructions regarding the handling of germs or biological material of
risk group II or higher, please refer to the "Laboratory Biosafety Manual"
(Source: World Health Organization, current edition of the Laboratory Biosafety
Manual).
WARNING! Risk to health from escaping substances.
Only use optically perfect and undamaged twin.tec PCR Plates.
Observe the maximum filling volume. Liquid may splash out when the selected
sealing system is removed.
Seal the twin.tec PCR Plates prior to centrifugation. Observe the instructions in
the device operating manual.
Store and transport the twin.tec PCR Plates in a way that prevents material from
escaping from the twin.tec PCR Plates.
Do not use the twin.tec PCR Plates as cryo tubes.
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Instructions for use
2
Eppendorf twin.tec
English (EN)
®
PCR Plates
Take appropriate measures to avoid contaminations after opening the bag containing
PCR clean consumables.
Reagents, consumables contaminated with reagents and materials used for cleaning
and disinfecting should be disposed of in accordance with laboratory regulations.
3 Application
For PCR instructions please refer to reagent manufacturers' work specifications or lab
protocols.
3.1 Filling
Maximum filling volume:
• twin.tec PCR Plate 96, semi-skirted and unskirted: 250 µL
• twin.tec PCR Plate 96, skirted and unskirted, low profile: 150 µL
• twin.tec PCR Plate 384: 45 µL
• twin.tec real-time PCR Plate 384: 40 µL
Fill the twin.tec PCR Plates directly or place them in an appropriate stand for filling.
3.2 Sealing
You can use cap strips, adhesive PCR foils and films or heat sealing foils and films to
seal the twin.tec PCR Plates.
We recommend heat sealing with Eppendorf Heat Sealing Foil or Eppendorf Heat
Sealing Film as the ideal protection against evaporation.
Perfect sealing of the PCR Plates allows an oil-free PCR reaction in a minimum volume of
up to 10 µL (96-well PCR Plates) or 5 µL (384-well PCR Plate).
3.3 Real-time PCR
An application for plates with white wells is to enhance the signal recovery and thus the
detection limit in real-time PCR applications. This is the case with a dye which has a low
concentration or low fluorescence intensity. Generally plates with white, signal amplifying
wells can cause irradiation - depending on the device - if the concentration of the dye is
too high. In this case, you should reduce the concentration of the probe or of the dye.
Page 3
Instructions for use
Eppendorf twin.tec
®
PCR Plates
English (EN)
4 Technical data
Material Polycarbonate, polypropylene (wells), autoclavable
Eppendorf PCR clean Certified free of RNase, DNase, human DNA and PCR inhibitors*.
Eppendorf LoBind Consumables with the highest recovery rates for DNA and RNA
Centrifugability Centrifugable up to 2 250 × g with PCR plate adapter in a
Storage Protect from direct sunlight and UV light. Store dry at room
Operational
temperature
* Lot-specific certificates are available at www.eppendorf.com or
certificates@eppendorf.de.
(121 °C, 20 min) in the non-sealed state.
molecules. PCR clean.
swing-bucket rotor.
twin.tec PCR Plate 96, unskirted, divisible: Centrifuge a maximum
of two plate segments per bucket and place them in the center of
the PCR plate adapter.
temperature.
-80 °C to +120 °C.
5 Ordering Information
Order no.
(International)
0030 128.648 951020401
0030 128.575 951020303
0030 133.307
0030 133.358
0030 133.366
0030 133.374
0030 133.412 0030133412
0030 132.513 951022015
Order no.
(North America)
0030133307
0030133358
0030133366
0030133374
Description
twin.tec PCR Plate 96, skirted
wells colorless, 25 pieces
low profile, clear
twin.tec PCR Plate 96, semi-skirted
wells colorless, 25 pieces
standard profile, clear
twin.tec PCR Plate 96 unskirted
wells colorless, 20 pieces
low profile, clear
low profile, divisible, clear
standard profile, clear
divisible, clear
twin.tec Adapter for LC 480
for PCR Plate 96, unskirted
twin.tec real-time PCR Plate 96 skirted
wells white, 25 pieces
white
3
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Instructions for use
Eppendorf twin.tec
English (EN)
®
PCR Plates
Order no.
(International)
Order no.
(North America)
Description
twin.tec real-time PCR Plate 96 semi-skirted
wells white, 25 pieces
0030 132.548 951022055
white
twin.tec real-time PCR Plate 96 unskirted
wells white, 20 pieces
0030 132.700 0030132700
low profile, white
twin.tec microbiology PCR Plate 96 skirted
wells colorless, 10 pieces
0030 129.300 0030129300
clear
twin.tec microbiology PCR Plate 96 semi-skirted
wells colorless, 10 pieces
0030 129.326 0030129326
clear
twin.tec PCR Plate 384
wells colorless, 25 pieces
0030 128.508 951020702
clear
twin.tec real-time PCR Plate 384
wells white, 25 pieces
0030 132.734 0030132734
white
twin.tec microbiology PCR Plate 384
wells colorless, 10 pieces
0030 129.342 0030129342
clear
twin.tec PCR Plate 96 LoBind skirted
wells colorless, 25 pieces
0030 129.512 0030129512
clear
twin.tec PCR Plate 96 LoBind semi-skirted
wells colorless, 25 pieces
0030 129.504 0030129504
clear
More ordering information regarding color variants of Eppendorf twin.tec PCR Plates,
Eppendorf PCR Film & Foil, Eppendorf PCR Tubes, PCR Tube Strips and PCR Cap Strips
(real-time and standard PCR):
www.eppendorf.com.
Patents
U.S. Patent No. 6,340,589
U.S. Patent No. 7,347,977
U.S. Patent No. 7,767,153
and the Eppendorf logo are registered trademarks of Eppendorf AG.
®
Your local distributor: www.eppendorf.com/contact
Eppendorf AG · 22331 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com
©2013 · Eppendorf
0012 573.893-03/082013
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