Eppendorf Tubes® 5.0 mL
Instructions for use
Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be
reproduced without the prior permission of the copyright owner.
®
Eppendorf
Biopur
Eppendorf Tubes
Flex-Tube
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and the Eppendorf logo are registered trademarks of Eppendorf AG.
®
, Eppendorf Research®, Eppendorf Reference®, Eppendorf Xplorer®,
®
, Eppendorf LoBind®, epT.I.P.S.®, ep Dualfilter T.I.P.S.® and
®
are registered trademarks of Eppendorf AG, Hamburg, Germany.
®
in this manual.
0012 581.033-00/042013
Intended use
®
Eppendorf Tubes
5.0 mL
English (EN)
1 Intended use
Eppendorf Tubes 5.0 mL are single-use tubes made from polypropylene for
preparing, mixing, centrifuging, transporting and storing solid and liquid
samples and reagents. The field of application is training, routine and research
laboratories in the areas of life sciences, industry, medical and pharmaceutical
research and, chemistry.
Operation requires skilled personnel who have been trained for the above
mentioned areas.
2 Safety notes
WARNING! Damage to health from toxic, radioactive or aggressive
chemicals as well as infectious liquids and pathogenic germs.
Observe the national regulations for handling these substances, the
biological security level of your laboratory, the material safety data
sheets and the manufacturer's application notes.
Wear personal protective equipment.
WARNING! Risk to health from escaping substances.
If the tube lids are not sealed properly or the lip seal is damaged, the
tube lid may burst open at increased temperatures.
3
Only use optically perfect and undamaged reaction tubes.
Seal the reaction tubes correctly. Do not damage the seal lip.
Observe the maximum filling volume. When opening the tubes, liquid
at the lid may splash out.
If the tube is heated >80 °C, use a clip.
Do not centrifuge any tubes which are sealed with a clip.
Make the necessary safety arrangements for the transport or storage
outside of the laboratory to prevent materials escaping from the tube.
The tubes must not be used with liquid nitrogen. The operating
temperature ranges from -86°C to +100°C. If the tubes were frozen in
liquid nitrogen, the tube lids may open up during thawing.
Safety notes
4
Eppendorf Tubes
English (EN)
®
5.0 mL
WARNING! For mixers or centrifuges: Risk of contamination if the
tube mounts are too large (wells, rotor bores, buckets).
Tubes may be destroyed and the substances they contain released.
Please note height and diameter of the tube. Only use suitable
centrifuge inserts or mixer thermoblocks
If required, use the adapter intended for this purpose.
WARNING! Risk of contamination from damaged tubes during
centrifugation.
The tubes withstand heavy loads during centrifugation. However, they
can be destroyed and release the substances they contain if used
improperly.
Close tube lids before commencing centrifugation. Open tube lids can
break off during centrifugation and damage the centrifuge.
Always observe the maximum permitted centrifugation forces.
Read the operating manual of the centrifuge used.
Please note that organic solvents can reduce the mechanical
resistance of the tubes. If in doubt, please contact the Eppendorf
Application Support (support@eppendorf.com).
WARNING! Risk of contamination from multiple use.
The tubes are intended for single use only.
Dispose of used tubes in accordance with the substances with which
they have come into contact.
WARNING! For sterile packaging: Risk of contamination due to
limited sterility of the tubes.
Tubes are only sterile in closed packaging. After five years the shelf life
expires even in closed packaging.
Only open the packaging immediately before use.
Observe the expiry date printed on the packaging.
Product description
Eppendorf Tubes
®
5.0 mL
English (EN)
CAUTION! Contamination risk when disposing of used consumables.
Dispose of used consumables in accordance with the substances with
which they have come into contact.
Observe the rules applicable to your lab.
NOTICE! Material damage from incorrect use.
Only use the product for its intended purpose as described in the
operating manual.
Ensure adequate material resistance when using chemical
substances.
If in doubt, contact the manufacturer of the product.
3 Product description
Eppendorf Tubes 5.0 mL are available in different purity grades (see p. 8).
Eppendorf Tubes 5.0 mL are equipped with a graduation as an indicator for the
filling quantity and a labeling area for identification.
Abb. 3-1: Tubes in a 5.0 mL tube rack
5
Fig. 3-1: Tubes in a 5.0 mL tube rack
Technical data
6
Eppendorf Tubes
English (EN)
®
5.0 mL
3.1 Use clip
If you are heating the tubes above 80 °C, seal them with a clip.
Do not centrifuge any tubes which are sealed with a clip.
1. Push the clip onto the tube from the
front.
2. Heat the tube at the required
temperature.
3. When the tube has cooled down,
remove the clip from the tube.
4 Technical data
Material: Polypropylene (colored, colorless)
Storage before use: Protect from direct sunlight and UV
light. Store in dry conditions at room
temperature.
Chemical resistance: refer to application no. 56: “The best
material for original Eppendorf Tubes®
and plates: properties and chemical
resistance of polypropylene"
Operating temperature: ≤80 °C, when using the clip ≤100 °C
Autoclavable: when opened, 121 °C, 20 min
Technical data
®
Eppendorf Tubes
5.0 mL
English (EN)
4.1 Maximum values for centrifuging
NOTICE! Tubes will get hot.
Depending on the the run time, g-force (rcf)/speed (rpm) and ambient
temperature, the temperature in the rotor chamber, rotor and in the
sample can increase to more than 40 °C.
Please note that the resistance to centrifugation of the tubes will
reduce.
Please note the temperature resistance of the samples.
Can be centrifuged at 4 °C – 40 °C
The Eppendorf Tubes 5.0 mL can be centrifuged with 25,000 x g:
• in a 45° fixed-angle rotor
• sample temperature of 40 °C with aqueous saline solution (1.0 g/mL density)
• when using suitable centrifuge adapters for 90 min
The mechanical strength of the tubes is reduced by the use of organic solvents.
In case of doubt, contact Eppendorf Application Support.
7
Technical data
8
Eppendorf Tubes
English (EN)
®
5.0 mL
4.2 Eppendorf purity grades
Eppendorf
Quality
Sterile PCR clean Biopur
Batch testing (certified) for the following purity criteria
Free of human DNA ■ ■
DNA-free (free of human
and bacterial DNA)
■
DNase-free ■ ■
RNase-free ■ ■
Free of PCR inhibitors ■ ■
ATP-free ■
Pyrogen-free
(endotoxin-free)
■■
Sterile (Ph.Eur./USP) ■ ■
Eppendorf Protein LoBind and DNA LoBind: consumables with the highest
recovery rates for proteins and DNA and RNA molecules. They are PCR clean,
i.e. free from RNase, DNase, human DNA and PCR inhibitors.
5 Ordering Information
Ordering Information
Eppendorf Tubes
English (EN)
®
5.0 mL
9
Order no.
(International)
Order no.
(North America)
Description
0030 119.401 0030119401 Eppendorf Tubes 5.0 mL
Eppendorf Quality, 200 pieces, clear
0030 119.460 0030119460 Eppendorf Tubes 5.0 mL
PCR clean, colorless, 200 pieces, clear
0030 119.487 0030119487 Eppendorf Tubes 5.0 mL
Sterile, 200 pieces, clear
Eppendorf Tubes 5.0 mL
Biopur, 50 pieces, clear
0030 119.479 0030119479
individually wrapped
0030 108.302 0030108302 Eppendorf Protein LoBind Tubes
5.0 mL
PCR clean, 100 pieces
0030 108.310 0030108310 Eppendorf DNA LoBind Tubes
5.0 mL
PCR clean, 200 pieces
0030 119.380 0030119380 Starter Pack Eppendorf Tubes
5.0 mL
PCR clean, 400 pieces, 2 Racks, 8
universal adapters
0030 119.509 0030119509 Tube Clip for Eppendorf Tubes
5.0 mL
10 pieces
Tube Rack
for Eppendorf Tubes 5.0 mL
0030 119.495 0030119495
2 pieces
Storage Box
for Eppendorf Tubes 5.0 mL
0030 127.943 0030127943
2 pieces
Ordering Information
10
Eppendorf Tubes
English (EN)
®
5.0 mL
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