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Eppendorf Tubes®
Instructions for use
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Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be
reproduced without the prior permission of the copyright owner.
Eppendorf
Biopur
Eppendorf Xplorer
ep Dualfilter T.I.P.S.
®
and the Eppendorf logo are registered trademarks of Eppendorf AG.
®
, Eppendorf®, Eppendorf Research®, Eppendorf Reference®,
®
, Eppendorf Tubes®, Eppendorf LoBind®, epT.I.P.S.®,
®
and Flex-Tube® are registered trademarks of Eppendorf
AG, Hamburg, Germany.
Registered trademarks are not marked in all cases with ™ or
®
in this manual.
0012 557.019-04/062013
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Intended use
Eppendorf Tubes®
English (EN)
Instructions for useDraftEppendorf Tubes®seeon p.Fig.Tab.p.
English (EN)Instructions for use
1 Intended use
Eppendorf Tubes are single-use tubes made from polypropylene for preparing,
mixing, centrifuging, transporting and storing solid and liquid samples and
reagents. The field of application is training, routine and research laboratories in
the areas of life sciences, industry, medical and pharmaceutical research and,
chemistry.
Operation requires skilled personnel who have been trained for the above
mentioned areas.
2 Safety notes
WARNING! Damage to health from toxic, radioactive or aggressive
chemicals as well as infectious liquids and pathogenic germs.
Observe the national regulations for handling these substances, the
biological security level of your laboratory, the material safety data
sheets and the manufacturer's application notes.
Wear personal protective equipment.
WARNING! Risk to health from escaping substances.
If the tube lids are not sealed properly or the lip seal is damaged, the
tube lid may burst open at increased temperatures.
3
Only use optically perfect and undamaged reaction tubes.
Seal the reaction tubes correctly. Do not damage the seal lip.
Observe the maximum filling volume. When opening the tubes, liquid
at the lid may splash out.
Make the necessary safety arrangements for the transport or storage
outside of the laboratory to prevent materials escaping from the tube.
The tubes must not be used with liquid nitrogen. The operating
temperature ranges from -86°C to +100°C. If the tubes were frozen in
liquid nitrogen, the tube lids may open up during thawing.
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Safety notes
4
Eppendorf Tubes®
English (EN)
WARNING! Risk of contamination from damaged tubes during
centrifugation.
The tubes withstand heavy loads during centrifugation. However, they
can be destroyed and release the substances they contain if used
improperly.
Close tube lids before commencing centrifugation. Open tube lids can
Always observe the maximum permitted centrifugation forces.
Read the operating manual of the centrifuge used.
Please note that organic solvents can reduce the mechanical
WARNING! Risk of contamination from multiple use.
The tubes are intended for single use only.
Dispose of used tubes in accordance with the substances with which
WARNING! For sterile packaging: Risk of contamination due to
limited sterility of the tubes.
Tubes are only sterile in closed packaging. After five years the shelf life
expires even in closed packaging.
break off during centrifugation and damage the centrifuge.
resistance of the tubes. If in doubt, please contact the Eppendorf
Application Support (support@eppendorf.com).
they have come into contact.
Only open the packaging immediately before use.
Observe the expiry date printed on the packaging.
CAUTION! Contamination risk when disposing of used consumables.
Dispose of used consumables in accordance with the substances with
which they have come into contact.
Observe the rules applicable to your lab.
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Product description
Eppendorf Tubes®
English (EN)
NOTICE! Material damage from incorrect use.
Only use the product for its intended purpose as described in the
operating manual.
Ensure adequate material resistance when using chemical
substances.
In case of any doubts, turn to the manufacturer of this product.
3 Product description
Eppendorf Tubes are available in the following product variations:
• Eppendorf Safe-Lock Tubes: tubes with lid latch for additional protection
against unintentional opening during tempering.
• Eppendorf Tubes 3810X and Flex-Tubes: standard tubes which meet high
requirements with regard to relative centrifugal force, temperature and
chemical resistance. They are easy to open and close.
Eppendorf Tubes are available in different purity grades (see p. 8).
Eppendorf Tubes are equipped with a graduation as an indicator for the filling
quantity and a labeling area for identification.
Abb. 3-1: Tubes in a 1.5/2.0 mL tube rack
5
Fig. 3-1: Tubes in a 1.5/2.0 mL tube rack
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Technical data
6
Eppendorf Tubes®
English (EN)
4 Technical data
Material: Polypropylene (colored, colorless)
Storage before use: Protect from direct sunlight and UV light. Store in
dry conditions at room temperature.
Chemical resistance: refer to application no. 56: “The best material for
original Eppendorf Tubes® and plates: properties
and chemical resistance of polypropylene"
Operating temperature: -86 °C - 100 °C
Autoclavable: when opened, 121 °C, 20 min
4.1 Maximum values for centrifuging
NOTICE! Tubes will get hot.
Depending on the the run time, g-force (rcf)/speed (rpm) and ambient
temperature, the temperature in the rotor chamber, rotor and in the
sample can increase to more than 40 °C.
Please note that the resistance to centrifugation of the tubes will
reduce.
Please note the temperature resistance of the samples.
Can be centrifuged at 4 °C – 40 °C
The tubes can be centrifuged with the maximum g-forces (rcf) listed in the table
below under the following conditions:
• in a 45° fixed-angle rotor
• sample temperature of 40 °C with aqueous saline solution (1.0 g/mL density)
• when using suitable centrifuge adapters for 90 min
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Technical data
Eppendorf Tubes®
English (EN)
7
Vessel Eppendorf
Biopur, sterile Protein LoBind
Quality,
PCR-clean,
DNA LoBind
Eppendorf
0.5 mL 30,000 × g 22,000 × g 18,000 × g
Safe-Lock Tubes
1,5 mL 30,000 × g 22,000 × g 18,000 × g
2.0 mL 25,000 × g 22,000 × g 18,000 × g
Eppendorf
1.5 mL 30,000 × g –
3810X Tubes
The mechanical strength of the tubes is reduced by the use of organic solvents.
In case of doubt, contact Eppendorf Application Support.
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Technical data
8
Eppendorf Tubes®
English (EN)
4.2 Eppendorf purity levels
Sterile +
PCR clean
Eppendorf
Quality
Lot testing (certified) for the following purity criteria
Human-DNA-free ■ ■ ■
DNA-free (Human +
Bacteria-DNA-free)
DNase-free ■ ■ ■
RNase-free ■ ■ ■
PCR-Inhibitor-free ■ ■ ■
ATP-free ■
Pyrogen-free
(Endotoxin-free)
Sterile (Ph.Eur./USP) ■ ■ ■
Sterile PCR clean
■■■
Biopur
■
Eppendorf Protein LoBind and DNA LoBind: consumables with the highest
recovery rates for proteins and DNA and RNA molecules. They are PCR clean,
i.e. free from RNase, DNase, human DNA and PCR inhibitors.
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5 Ordering Information
5.1 Spare parts
Ordering Information
Eppendorf Tubes®
English (EN)
9
Order no.
(International)
0030 121.023
0030 121.708
0030 121.112
0030 121.120
0030 121.139
0030 121.147
0030 121.155
0030 120.086
0030 121.694
0030 120.159
0030 120.167
0030 120.175
0030 120.183
0030 120.191
0030 120.094
0030 121.686
0030 120.205
0030 120.213
0030 120.221
0030 120.230
0030 120.248
0030 121.570
Description
Eppendorf Safe-Lock Tube 0.5 mL
500 pieces
clear
assortment
yellow
red
blue
green
amber
Eppendorf Safe-Lock Tube 1.5 mL
1,000 pieces
clear
assortment
yellow
red
blue
green
amber
Eppendorf Safe-Lock Tube 2.0 mL
1,000 pieces
clear
assortment
yellow
red
blue
green
amber
Eppendorf Safe-Lock Tube 0.5 mL
50 pieces
Biopur, individually sealed
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Ordering Information
10
Eppendorf Tubes®
English (EN)
Order no.
(International)
Description
Eppendorf Safe-Lock Tube 1.5 mL
100 pieces
0030 121.589
Biopur, individually sealed
Eppendorf Safe-Lock Tube 2.0 mL
100 pieces
0030 121.597
Biopur, individually sealed
Eppendorf Safe-Lock Tube 0.5 mL
500 pieces, clear
0030 123.301
PCR clean
Eppendorf Safe-Lock Tube 1.5 mL
1,000 pieces, colorless
0030 123.328
PCR clean
Eppendorf Safe-Lock Tube 2.0 mL
1,000 pieces, colorless
0030 123.344
PCR clean
Micro test tube 3810X 1.5 mL
1,000 pieces
0030 125.150
clear
Micro test tube 3810X 1.5 mL
1,000 pieces, clear
0030 125.215
PCR clean
0030 108.094 Eppendorf Protein LoBind Tubes 0.5 mL
PCR clean, 100 pieces
0030 108.116 Eppendorf Protein LoBind Tubes 1.5 mL
PCR clean, 100 pieces
0030 108.132 Eppendorf Protein LoBind Tubes 2.0 mL
PCR clean, 100 pieces
0030 108.035 Eppendorf DNA LoBind Tubes 0.5 mL
PCR clean, 250 pieces
0030 108.051 Eppendorf DNA LoBind Tubes 1.5 mL
PCR clean, 250 pieces
0030 108.078 Eppendorf DNA LoBind Tubes 2.0 mL
PCR clean, 250 pieces
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