Eppendorf TransferMan4 r User Manual

Download

www.eppendorf.com/myeppendorf

TransferMan® 4r

Operating manual

Eppendorf

and the Eppendorf logo are registered trademarks of Eppendorf AG,

Hamburg, Germany.

Tran sferMan

4r, FemtoJet® and Eppendorf PiezoXpert® are registered trademarks of

Eppendorf AG, Hamburg, Germany.

Registered trademarks are not marked in all cases with ™ or ® in this manual.

No part of this publication may be reproduced without the prior permission of the copyright owner.

5193 900.013-00/072013

Tran sferMan

English (EN)

Operating manualTransferMan® 4 rseeon p.Fig.Tab.p.

English (EN)Operating manual

Table of contents

1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.3.1 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.3.2 Control board. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.4 Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

2.5 Joystick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.5.1 Proportional range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.5.2 Dynamic range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.5.3 Direction of movement of the joystick. . . . . . . . . . . . . . . . . . . . . . 16

2.5.4 Direction of movement of the rotating wheel . . . . . . . . . . . . . . . . 17

2.5.5 Joystick key functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

2.6 Working range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

2.7 Resulting speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.3 Warning signs on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.1.1 If there is any damage, make a claim . . . . . . . . . . . . . . . . . . . . . . 22

4.1.2 Delivery incomplete . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.1.3 Microscope adapter assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.3 Mounting overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.3.1 Module (X, Y, Z). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

4.3.2 Z-module holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4.3.3 Slider . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4.3.4 Angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

4.3.5 Swivel joint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Table of contents

Tran sferMan

English (EN)

4.4 Mounting the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

4.4.1 Mounting the Z-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

4.4.2 Mounting the Y-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

4.4.3 Mounting the X-module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

4.4.4 Mounting the angle head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

4.5 Inserting the capillary holder into the angle head . . . . . . . . . . . . . . . . . . . . 31

4.6 Inserting the capillary into the capillary holder . . . . . . . . . . . . . . . . . . . . . . 31

4.6.1 Inserting Femtotips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

4.7 Setting the injection angle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

4.8 Aligning the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

4.8.1 Aligning the height . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

4.8.2 Aligning the depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

4.8.3 Aligning the width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

4.8.4 Aligning the angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

4.9 Entering mounting parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

4.9.1 Microscope and adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

4.9.2 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

4.9.3 Angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

4.10 Converting the swivel joint for left side mounting . . . . . . . . . . . . . . . . . . . . 36

4.11 Converting the angle head for left side mounting . . . . . . . . . . . . . . . . . . . . 39

4.12 Connect the motor module to the control board. . . . . . . . . . . . . . . . . . . . . . 40

4.13 Setting installation parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

4.13.1 First set-up wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

4.14 Connecting an external device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

4.14.1 Connecting the FemtoJet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

4.14.2 Connecting the PiezoXpert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

4.14.3 Connecting the computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

4.14.4 Connecting two devices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.1 Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.1.1 Application display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.1.2 Display of coordinates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

5.1.3 Menu display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

5.2 Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.2.1 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.2.2 Application – Cell transfer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.2.3 Application – ICSI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.2.4 Application – DNA injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

5.2.5 Application – Basic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

5.2.6 Application – My application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

5.3 Main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

5.4 Navigating the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

5.4.1 Entering or changing parameters . . . . . . . . . . . . . . . . . . . . . . . . . 54

Table of contents

Tran sferMan

English (EN)

6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.1 Switching the device on or off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.1.1 Switching on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.1.2 Switching the device off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.2 Activating or deactivating the control board. . . . . . . . . . . . . . . . . . . . . . . . . 55

6.2.1 Activating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.2.2 Deactivating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.3 Defining the start screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.3.1 Defining the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.3.2 Defining the selected application . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.4 Replacing the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.4.1 Manually positioning the capillary . . . . . . . . . . . . . . . . . . . . . . . . 57

6.4.2 Automatically positioning the capillary . . . . . . . . . . . . . . . . . . . . . 57

6.5 Changing the sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

6.6 Changing the size of the working range . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

6.6.1 Change parameters using the selection dial . . . . . . . . . . . . . . . . . 58

6.6.2 Changing parameters in the menu . . . . . . . . . . . . . . . . . . . . . . . . 59

6.7 Shifting the movement range of the capillary. . . . . . . . . . . . . . . . . . . . . . . . 59

6.7.1 Extending the movement range into the dynamic range . . . . . . . 59

6.7.2 Uncoupling and resetting the joystick. . . . . . . . . . . . . . . . . . . . . . 59

6.8 Capillary positions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

6.8.1 Saving a position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

6.8.2 Move to the position using the softkey . . . . . . . . . . . . . . . . . . . . . 60

6.8.3 Move to the position using the joystick button . . . . . . . . . . . . . . . 61

6.8.4 Deleting a stored position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.8.5 Overwriting a stored position . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.9 Vertical limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.9.1 Defining the lower limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.9.2 Deleting the lower limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.9.3 Defining the upper limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.9.4 Deleting the upper limit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

6.10 Horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

6.10.1 Defining the horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

6.10.2 Deleting the horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

6.11 Speed function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

6.11.1 Speed menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

6.11.2 Setting the parameter for Speed . . . . . . . . . . . . . . . . . . . . . . . . . . 64

6.12 Step injection function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

6.12.1 Step injection menu and parameters. . . . . . . . . . . . . . . . . . . . . . . 65

6.12.2 Executing the Step injection function . . . . . . . . . . . . . . . . . . . . . . 66

Table of contents

Tran sferMan

English (EN)

6.13 Home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.13.1 Home menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.13.2 Setting the parameter for Home . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.13.3 Move the capillary out with the home key . . . . . . . . . . . . . . . . . . 67

6.13.4 Move the capillary back with the home key . . . . . . . . . . . . . . . . . 67

6.13.5 Setting the offset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

6.13.6 Terminate the home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

6.14 Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

6.14.1 Clean menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

6.14.2 Setting the parameter for Clean . . . . . . . . . . . . . . . . . . . . . . . . . . 69

6.14.3 Execute the Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

6.14.4 Terminate the Clean function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

6.15 PiezoXpert function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

6.15.1 PiezoXpert menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . 70

6.15.2 Execute the PiezoXpert function . . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16 Installation function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16.1 Installation menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16.2 Installation parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16.3 Control board parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

6.16.4 Joystick parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

6.16.5 Module parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

6.17 Function function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.17.1 Function menu and parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.17.2 Executing Zero coordin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.17.3 Executing Center motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.17.4 Executing User default . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

6.18 Softkeys function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

6.18.1 Softkeys menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

6.18.2 Execute Softkeys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

6.18.3 Execute Joystick key . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

6.19 Change appl. function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

6.19.1 Change appl. menu and parameters . . . . . . . . . . . . . . . . . . . . . . . 76

6.19.2 Defining the selected application as the start screen . . . . . . . . . . 77

6.19.3 Defining the application as the start screen . . . . . . . . . . . . . . . . . 77

6.20 Service function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

6.20.1 Service menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

6.20.2 Execute the Selftest function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

6.21 Resetting parameters to the factory settings . . . . . . . . . . . . . . . . . . . . . . . . 78

6.21.1 Perform reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

6.21.2 Carrying out a reset in the menu . . . . . . . . . . . . . . . . . . . . . . . . . . 78

6.22 Remote computer control of the TransferMan 4r . . . . . . . . . . . . . . . . . . . . . 78

Table of contents

Tran sferMan

English (EN)

7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.1 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.2 Capillary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.3 Control board and display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.4 Joystick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.1.5 Software and parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.2.1 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.2.2 Error. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

8 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.1 Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.3 Service and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

8.4 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

9.2 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

9.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.1 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.3 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.4 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

11 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

11.1 TransferMan 4r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

11.2 Accessories for TransferMan 4r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

11.3 Tools for TransferMan 4r. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

11.4 Microscope adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

11.5 Accessories for adapters for microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

11.6 Capillaries for research applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

11.7 Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

11.8 Universal capillary holder and accessories. . . . . . . . . . . . . . . . . . . . . . . . . . 91

11.9 CellTram and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

11.10 FemtoJet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

11.11 FemtoJet express. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

11.12 Accessories for FemtoJet/FemtoJet express . . . . . . . . . . . . . . . . . . . . . . . . . 93

11.13 PiezoXpert. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

Operating instructions

Tran sferMan

English (EN)

1 Operating instructions

1.1 Using this manual

 Read this operating manual completely before using the device for the first time.

Please also note the operating instructions for the accessories, if applicable.

 This operating manual is part of the product. Thus, it must always be easily accessible.  Enclose this operating manual when transferring the device to third parties.  If this manual is lost, please request another one. You will find the current version on

our webpage www.eppendorf.com/worldwide

1.2 Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1 Danger symbols

Hazard point Cuts

Material damage Electric shock

Biohazard

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WAR NIN G Can lead to severe injuries or death.

CAUTION Can lead to light to moderate injuries.

ATTENTION May lead to material damage.

1.3 Symbols used

Depiction Meaning

Actions in the specified order

 Actions without a specified order

• List:

Text Display or software texts

Additional information

2 Product description

2.1 Delivery package

Quantity Description

1X-module

1Y-module

1Z-module

1 YZ-connector

1 Swivel joint

1 Angle head

1 Control board

1Mains/power cord

1Cable sheathing

1 Operating manual

2.1.1 Tools

Quantity Description

7 Allen key

1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6 mm

1 3 mm Allen torque key

1 1.3 mm Allen key

1 Tool bag

Product description

Tran sferMan

English (EN)

2.1.2 Accessories

Quantity Description

1 Connecting cable for FemtoJet/FemtoJet express

2 Positioning aids for capillary holders

1 Spare parts kit

1Label

2.2 Features

The micromanipulator TransferMan 4r has been especially developed for work processes that require intuitive movement of the capillary.

The TransferMan 4r combines the classical benefits of a mechanical system with the benefits of an accurate electrically driven system.

The capillary is controlled by a joystick. The joystick has an inner (proportional) and an

Product description

Tran sferMan

English (EN)

outer (dynamic) movement range. In the inner range, the joystick movement is transferred directly to the capillary. In the outer range, a greater forwards or backwards movement of the joystick results in an acceleration of the capillary movement. The movement ranges enable moving to any position in the working range of the micromanipulator.

Proportional movement is suitable for all working techniques that require intuitive, sensitive handling, e.g., intracytoplasmic sperm injection (ICSI) and the transfer of stem cells into blastocysts.

The software control provides predefined applications, freely programmable softkey functions, a freely programmable application and the storage of different positions in all space coordinates.

2.3 Main illustration

The motor module is mounted on a special microscope adapter or on a free-standing stand (magnetic). The control board is mechanically separated from the motor module.

Abb. 2-1:TransferMan 4r — right side mounting

Fig. 2-1: TransferMan 4r — right side mounting

1 Motor module 2 Control board

Product description

Tran sferMan

English (EN)

2.3.1 Motor module

The motor module is made up of three modules. The capillary can be moved in all three spatial axes due to the layout of the modules. The X-module with the capillary can be swung out of the work area using the swivel joint. The injection angle of the capillary can be set to any angle on the angle head.

Abb. 2-2:Motor module

Fig. 2-2: Motor module

1Z-module

2YZ-connector

3 Angle head

4Y-module

5Swivel joint

6X-module

Product description

home

fine

coarse

Tran sferMan

English (EN)

2.3.2 Control board

The control board contains the key pad, the display and the joystick, and, to the side, the selection dial. The direction of movement and the speed of the joystick are transferred to the capillary. The responsitivity of the movement and the size of the working range are predefined in the software setting. On the control panel, the working range can be selected and individually changed using the selection dial.

Abb. 2-3:Control board - Front

Fig. 2-3: Control board - Front

1Joystick

Proportional and dynamic movement

2Display

3Control panel

4 Selection dial

For increasing or reducing the working range

Abb. 2-4:Control board - rear

X-Axis

Y-Axis

ext. Device

Z-Axis

SERVICE

Fig. 2-4: Control board - rear

Product description

Tran sferMan

English (EN)

1Mains power switch On/Off

2 Connection for an external device

Foot control, FemtoJet, FemtoJet express or PiezoXpert

3 Connection for a Y-module

4 Connection for an X-module

5 Connection for a Z-module

6 Service interface

7 Mains/power connection

8Fuse

Product description

Tran sferMan

English (EN)

2.4 Control panel

With the keys on the control panel you can switch on the control board and select the size of the working range. Via the softkeys you can open the applications, execute the functions, navigate the menu and set the parameters.

Abb. 2-5:Control panel

Fig. 2-5: Control panel

1 home key

Moves the capillary out of the working range to a defined position

2Display

Displays the software

3 standby key

Switches the control board on or off or cancels automatic movements

4Softkeys 1 – 5

For selecting the application, triggering the function, navigating or setting parameter values

fine

x–fine

homecoarse

5 menu key

Opens the menu

6 fine/x-fine key

Sets the medium or small working range

7 coarse key

Sets the large working range

Product description

Tran sferMan

English (EN)

2.5 Joystick

The joystick moves the capillary in all three spatial axes. The joystick movement is transferred directly to the capillary in the proportional range. In the dynamic range, the movement of the capillary is accelerated the further the joystick is moved forwards and backwards.

Abb. 2-6:Joystick

Fig. 2-6: Joystick

1Joystick key

2Swivel

2Lower part

Controls movements in the X and Y-axis

Controls movements in the Z-axis

Abb. 2-7:Movement ranges of the joystick

Fig. 2-7: Movement ranges of the joystick

1 Proportional range 2 Dynamic range

Product description

Tran sferMan

English (EN)

2.5.1 Proportional range

In the proportional range, the capillary will move as quickly or slowly as the joystick is moved. The travel of the capillary is also proportional to the distance that the joystick has been moved forwards and backwards. The movement of the capillary will stop as soon as the joystick is no longer moved or when the position is reached at which the joystick was stopped. There is a noticeable stop on the outer limit of the proportional range. This stop is in a narrow zone in which moving the joystick sideways does not result in any further movement of the capillary.

The size of the proportional range depends on the working range selected.

2.5.2 Dynamic range

After the stop, the dynamic range of the joystick begins: when the joystick is moved against the spring-loaded stop, the capillary starts moving in the direction of the joystick movement. The movement stops when the joystick is released and it drops back into the zone due to the spring force of the stop. The speed of the capillary in the dynamic range is increased dynamically by a stronger pressure against the stop.

The size of the dynamic range is limited by the movement range of the modules (X, Y and Z).

2.5.3 Direction of movement of the joystick

The joystick can be moved along the horizontal plane. This controls the motors of the X-module and the Y-module. The joystick can be moved in one axis at a time or in a combination of axes.

Abb. 2-8:Movement in the X and Y-axis

 Move the capillary in the horizontal

direction (X and Y-axis).

Fig. 2-8: Movement in the X and Y-axis

Product description

Tran sferMan

English (EN)

2.5.4 Direction of movement of the rotating wheel

The rotating wheel on the joystick moves the motor module in the vertical axis. This activates the motor of the Z-module.

Abb. 2-9:Movement in the Z-axis

 Move the capillary in the vertical

direction (Z-axis).

Fig. 2-9: Movement in the Z-axis

2.5.5 Joystick key functions

Abb. 2-10:No movement of the capillary

 Uncouple the joystick from the motor

module.

 Trigger functions (e.g., switch between

saved positions).

Fig. 2-10: No movement of the capillary

2.6 Working range

There are three working ranges within the movement range of the joystick. For each working range, a radius with a resulting speed ratio is preset. The radius can be set using the selection dial on the control board and in the Speed menu.

Working ranges:

• coarse – for a large working range

• fine – for a medium working range

• x-fine – for a small working range

Product description

Tran sferMan

English (EN)

Abb. 2-11:Large working r ange –

Fig. 2-11: Large working range – coarse

Abb. 2-12:Medium working ra nge –

Fig. 2-12: Medium working range – fine

Abb. 2-13:Small working ran ge –

 Move capillary over a large distance.  Position capillary roughly and quickly.

 Move capillary over a medium distance.  Position capillary precisely.

 Move capillary over a very short

distance.

 Position capillary very precisely and

slowly.

 Active for x-fine working range larger

than 0.

Fig. 2-13: Small working range – x-fine

Product description

Tran sferMan

English (EN)

2.7 Resulting speed

In the inner (proportional) range, the speed of the capillary is dependent on the speed at which the joystick is moved forwards and backwards and the size of the set working range. If the joystick is moved forwards and backwards at the same speed with, e.g., the setting fine or coarse, the resulting speed with the smaller fine working range is less than with the larger coarse range.

The speed of the outer (dynamic) range is coupled to the selected working range. The coupling factor (Dyn-factor) can be changed and adjusted in the Installation menu. Since the speed results from the working range, the settings for Coarse fine and x-fine can be adjusted in the Speed menu.

Safety

Tran sferMan

English (EN)

3Safety

3.1 Intended use

The TransferMan 4r has been designed and manufactured for use in biological, chemical and physical research. It is used for the precise positioning of microcapillaries and similar tools as well as for the transfer of very small sample volumes.

The TransferMan 4r has been designed and manufactured for research use only.

The TransferMan 4r is intended exclusively for indoor use and for operation by qualified staff.

3.2 Warnings for intended use

WARNING! Risk of injury due to flying capillaries and glass splinters.

If exposed to high pressures, capillaries may detach themselves from the grip heads and become projectiles. Capillaries can crack as a result of incorrect handling.

 Wear protective goggles.  Never aim capillaries at people.  Use capillaries with an outer diameter that matches the grip head

specifications.

 Always mount / dismount capillaries when they are depressurized.  Mount the capillary correctly in the grip head.  Do not touch the capillary with the Petri dish or other objects.

CAUTION! Risk of cuts from broken capillaries.

Capillaries are made of glass and are very fragile.

 Wear your personal protective equipment (PPE).  Always mount capillaries depressurized.  Never aim capillaries at people.  Handle the capillaries very carefully.

NOTICE! Mechanical damage to the motor modules.

Excessive load leads to increment errors or destruction of the drive.

 Do not drive the modules against mechanical obstructions.  Do not hold any objects near the modules.  Load the motor module with a maximum of 100 g.

WARNING! Damages to health due to infectious liquids and pathogenic germs.

 When handling infectious liquids and pathogenic germs, observe the national

regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes.

 Wear personal protective equipment.  Consult the "Laboratory Biosafety Manual" (Source: World Health

Organization, Laboratory Biosafety Manual, as amended) for comprehensive regulations on the handling of risk group II germs or biological materials.

3.3 Warning signs on the device

Warning symbol Meaning

Warns of the risk of injury caused by capillary tips

Warns of the danger of crushing on the motor module

Tran sferMan

Safety

English (EN)

Read the operating manual

3.4 Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

• The device is not used in accordance with the operating manual.

• The device is used outside of its intended use.

• The device is used with accessories or consumables which are not recommended by Eppendorf.

• The device is maintained or repaired by people not authorized by Eppendorf.

• The user makes unauthorized changes to the device.

Installation

Tran sferMan

English (EN)

4Installation

4.1 Preparing installation

NOTICE! Damage to the control board as a result of incorrect handling.

 Grasp the control board on the housing.  Do not lift the control board using the joystick.  Never place the control board on the joystick.

Keep the packaging and the transport securing devices for later transport or storage.

Do not operate the device if there is visible damage to the device itself and/or to its packaging.

1. Check the packaging for damage.

2. Carefully remove the motor module and the control board from the packaging.

3. Check that everything is included in the delivery.

4. Check the modules, the control board and the accessories for damage.

4.1.1 If there is any damage, make a claim

1. Contact customer service.

4.1.2 Delivery incomplete

1. Contact customer service.

4.1.3 Microscope adapter assembly

The microscope adapter is not included in the scope of delivery and must be ordered separately.

1. Assemble the microscope adapter in accordance with the assembly instructions for the microscope adapter.

4.2 Selecting the location

Select the location for the device according to the following criteria:

• Mains connection in accordance with the name plate.

• A bench with a horizontal and even work surface which is designed to support the weight of the device.

• A mat or table that is cushioned against vibrations.

• The location is protected from direct sunlight and drafts.

The mains/power switch and cutting unit of the mains/power line must be easily accessible during operation (e.g, residual current circuit breaker).

4.3 Mounting overview

5 6

8910

Abb. 4-1:Overview for right-hand side mounting

Fig. 4-1: Overview for right-hand side mounting

Installation

Tran sferMan

English (EN)

1 Z-module holder

For left side mounting

2Microscope adapter

3 Z-module holder

For right side mounting

4Z-module

5Y-module

6Swivel joint

7X-module

8 Universal capillary holder

(not included in the delivery package)

9Angle head

10 YZ-connector

Installation

Tran sferMan

English (EN)

4.3.1 Module (X, Y, Z)

Abb. 4-2:Y-module

Fig. 4-2: Y-module

1Cable

2Module identification

3Movable rail

4Scale

Movement range of the rail

5Fixed rail

4.3.2 Z-module holder

Abb. 4-3:Z-module holder, front and back

Fig. 4-3: Z-module holder, front and back

Installation

Tran sferMan

English (EN)

1Slider

2Screw

Attach Z-module

4.3.3 Slider

Abb. 4-4:Slider

Fig. 4-4: Slider

1Slider

2Lock washer

3Screw

Attach Z-module holder to the adapter

4 Stop angle

3Flat washer

4Screw

Installation

Tran sferMan

English (EN)

4.3.4 Angle head

Abb. 4-5:Angle head

Fig. 4-5: Angle head

1 Identification

For setting the angle

2Knurled screw

For setting the injection angle

3 Universal capillary holder

(not included in the delivery package)

4 Positioning aid

5Knurled screw

For fixing the universal capillary holder

4.3.5 Swivel joint

Abb. 4-6:Swivel joint for right side mounting

Installation

Tran sferMan

English (EN)

6 7

Fig. 4-6: Swivel joint for right side mounting

1Slider

Y-module

2Turntable

3Allen screws

4Slider

X-module

5Stop plate

6Upper joint

7Mounting mark

| stands for left side mounting || stands for right side mounting

8Lower joint

4.4 Mounting the motor module

The motor module can be mounted on the right-hand or left-hand side of the microscope adapter. The following describes mounting on the right-hand side. For mounting on the left-hand side, the swivel joint and the angle head must be modified.

The motor module is mounted as standard on an inverse microscope. It can also be mounted on a universal stand. Mounting on a universal stand is described in the corresponding manual.

Installation

Tran sferMan

English (EN)

4.4.1 Mounting the Z-module

Prerequisites

• 3 mm Allen torque key

1. Push the Z-module holder onto the microscope adapter and tighten.

2. Push the fixed rail onto the Z-module holder until it reaches the stop angle and tighten the screw. The connecting cable faces to the rear.

4.4.2 Mounting the Y-module

Prerequisites

• 3 mm Allen torque key

Installation

Tran sferMan

English (EN)

1. Push the YZ-connector onto the fixed rail of the Y-module and tighten.

2. Push the Y-module with the YZ-connector onto the Z-module and tighten.

4.4.3 Mounting the X-module

Prerequisites

• 3 mm Allen torque key

1. Push the swivel joint onto the fixed rail of the X-module and tighten.

Installation

Tran sferMan

English (EN)

4.4.4 Mounting the angle head

Prerequisites

• 3 mm Allen torque key

2. Push the swivel joint with the X-module onto the Y-module and tighten.

1. Push the angle head onto the X-module.

2. Slightly tighten the angle head.

Installation

Tran sferMan

English (EN)

4.5 Inserting the capillary holder into the angle head

Prerequisites

• A universal capillary holder from Eppendorf is available.

• A capillary holder (4 mm diameter) from a different manufacturer is available.

1. Loosen the knurled screw on the angle head.

2. Insert the capillary holder into the clamp.

3. Align the capillary holder in such a way that the capillary tip is located approx. 20 mm above and approx. 20 mm from the outside of the operating point.

4. Place the positioning aid on the capillary holder and tighten.

5. Tighten the knurled screw.

4.6 Inserting the capillary into the capillary holder

WARNING! Risk of injury due to flying capillaries and glass splinters.

If exposed to high pressures, capillaries may detach themselves from the grip heads and become projectiles. Capillaries can crack as a result of incorrect handling.

 Wear protective goggles.  Never aim capillaries at people.  Use capillaries with an outer diameter that matches the grip head

specifications.

 Always mount / dismount capillaries when they are depressurized.  Mount the capillary correctly in the grip head.  Do not touch the capillary with the Petri dish or other objects.

NOTICE! Mechanical damage to the motor modules.

Excessive load leads to increment errors or destruction of the drive.

 Do not drive the modules against mechanical obstructions.  Do not hold any objects near the modules.  Load the motor module with a maximum of 100 g.

Installation

Tran sferMan

English (EN)

Standard capillary: Only use the 0 grip head with capillaries with an outer diameter of 1.0 mm to 1.1 mm. If you would like to use other capillaries, order the matching grip head.

1. Push the capillary into the grip head until it reaches the stop and tighten the grip head.

4.6.1 Inserting Femtotips

1. Remove the grip head.

2. Screw the adapter for Femtotips into the universal capillary holder.

3. Screw the Femtotip into the adapter and tighten.

4.7 Setting the injection angle

1. Turn the knurled screw to set the injection angle.

Installation

Tran sferMan

English (EN)

4.8 Aligning the motor module

Centrally align the modules in order to be able to use the full movement range of the modules.

4.8.1 Aligning the height

1. Undo the screw on the YZ-connector.

2. Align the Y-module on the scale of the Z-module and tighten the screw.

4.8.2 Aligning the depth

1. Undo the screw on the swivel joint.

2. Align the X-module on the scale of the Y-module and tighten the screw.

Installation

Tran sferMan

English (EN)

4.8.3 Aligning the width

4.8.4 Aligning the angle head

1. Undo the screw on the Z-module holder.

2. Align the Z-module on the scale of the microscope adapter and tighten the screw.

1. Undo the screw on the angle head.

2. Align the angle head on the scale of the X-module.

4.9 Entering mounting parameters

To facilitate remounting the mounting parameters can be recorded.

1. Enter the mounting parameters in the tables.

4.9.1 Microscope and adapter

Name Type

Microscope

Adapter

Attachment side of the motor module

Installation

3 4

Tran sferMan

English (EN)

4.9.2 Motor module

Position Description Position [mm]

1 Z-module holder on the adapter

2 Z-module on the Z-module holder

3 YZ-connector on the Z-module

4 YZ-connector on the Y-module

5 Swivel joint on the Y-module

6 Swivel joint on the X-module

7 Angle head on the X-module

4.9.3 Angle head

Name Position [mm] Degrees

Capillary holder

Injection angle

Installation

Tran sferMan

English (EN)

4.10 Converting the swivel joint for left side mounting

Prerequisites

• 2 mm Allen key

• Right side mounting marks (||) are aligned above each other

1. Rotate the lower joint until both Allen screws are accessible.

2. Unscrew the Allen screws.

Tran sferMan

English (EN)

3. Remove the stop plate.

4. Rotate the lower joint back.

5. Rotate the upper joint by 180°. The sliders must be at a 90° angle to each other.

Installation

Tran sferMan

English (EN)

6. Align the left side mounting marks (I).

7. Fit the stop plate in such a way that the pins sit in the holes of the turntable.

8. Rotate the swivel joint by 180°.

9. Screw down the stop plate.

• Left side mounting marks (|) are aligned above each other.

10.Check the position of the joints.

• The sliders must be at a 90° angle to each other.

90°

4.11 Converting the angle head for left side mounting

Prerequisites

• 1.3 mm Allen key

1. Undo the set screw and pull the knurled screw off the spindle.

Installation

Tran sferMan

English (EN)

Installation

Tran sferMan

English (EN)

2. Rotate the angle head by 180°.

3. Push the knurled screw onto the spindle end and fasten it with the set screw.

4. Turn the knurled screw until the desired

angle is set.

4.12 Connect the motor module to the control board

WARNING! Risk from incorrect supply voltage

 Only connect the device to power supplies which correspond with the

electrical requirements on the name plate.

 Only use sockets with a protective earth (PE) conductor and suitable power

cable.

NOTICE! Damage to the control board as a result of incorrect handling.

 Grasp the control board on the housing.  Do not lift the control board using the joystick.  Never place the control board on the joystick.

Installation

Tran sferMan

English (EN)

NOTICE! Material damage from incorrect connections.

 Only electrical connections may be made to devices described in the

operating manual.

 Other connections are permitted only following consultation and agreement

with Eppendorf AG.

 Only connect devices that meet the safety requirements defined in

IEC 60950-1.

NOTICE! Short circuit caused by incorrect installation.

 Failure to observe the order of steps may result in a short circuit.

Prerequisites

• TransferMan 4r is switched off.

• The power cable is disconnected.

1. Connect the module (X,Y,Z) plug with the ports on the control board.

2. Tighten the fixing screws on the plug manually.

3. Connect the power cable.

4. Switch on the mains switch.

5. Set the installation parameters. You can use the software wizard First set-up or the

Installation menu to set the Side and Angle parameters.

4.13 Setting installation parameters

Installation parameters must be set:

• for the initial installation

•after a reset

The following settings are defined:

• Mounting side of the motor module

• Operating angle for the capillary

• Center motors

• Adjust motors

•Set date

• Dynamic movement range of the joystick

Installation

Tran sferMan

English (EN)

4.13.1 First set-up wizard

Prerequisites

• The micromanipulator is switched on.

1. Select the First set-up application.

2. Select the mounting side.

3. Select Next.

4. Set the operating angle.

5. Select Next.

Installation

Tran sferMan

English (EN)

6. Select Execute to move all motors to the middle position.

7. Select Next.

8. Insert the capillary holder into the angle head.

9. Select Next.

10.Manually align the modules.

11.Select Next.

12.Remove the capillary holder.

13.Insert the capillary into the capillary holder.

14.Insert the capillary holder with the capillary into the angle head.

15.Finely adjust the position of the capillary holder and the modules.

16.Select Next.

Installation

Tran sferMan

English (EN)

17.Set the time and the date.

18.Select Next.

19.Switch the dynamic movement range of the joystick on or off.

20.Select Next.

21.Select Enter.

• The installation is complete and the modules have been adjusted.

• The application screen shows My application.

Installation

Tran sferMan

English (EN)

4.14 Connecting an external device

The following devices can be connected to the control board:

• EppendorfFemtoJet

• EppendorfPiezoXpert

•Computer

4.14.1 Connecting the FemtoJet

Prerequisites

• Devices are switched off.

The operation is described in the manual for the FemtoJet.

1. Connect the FemtoJet to the port for external devices.

2. Switch on the FemtoJet. The initialization phase starts.

3. Switch on the control board.

• After completion of the initialization phase, the status message Injector ready appears on the application screen.

4.14.2 Connecting the PiezoXpert

Prerequisites

• Devices are switched off.

The operation is described in the manual for the PiezoXpert.

1. Connect the PiezoXpert to the port for external devices.

2. Switch on the PiezoXpert. The initialization phase starts.

3. Switch on the control board.

• After completion of the initialization phase, the status message PiezoXpert ready appears on the application screen.

Installation

Tran sferMan

English (EN)

4.14.3 Connecting the computer

Prerequisites

• A data cable is available.

• Devices are switched off.

Control with a computer is described in the Cell Technology · PC Control manual.

1. Connect the data cable to the port for external devices.

2. Connect the computer to the data cable.

3. Switch on the control board.

4.14.4 Connecting two devices

Prerequisites

• Y-connector is available.

• Devices are switched off.

Two devices can be connected with a Y-connector.

The following combinations are possible:

• Computer and FemtoJet

• FemtoJet and PiezoXpert

1. Connect the Y-connector to the port for external devices.

2. Connect the device combination.

3. Connect the devices.

• After initialization, status messages appear on the application screen.

Software

Tran sferMan

English (EN)

5 Software

5.1 Display

The display shows current settings, e.g., the selected working range, the position of the motors and the defined limits.

5.1.1 Application display

Abb. 5-1:Display layout - ICSI application example

Fig. 5-1: Display layout - ICSI application example

1 Status line with working range

2 Active application with speed bars

3 Connected device

4 Softkey status fields

5Softkeys

6 Function of the joystick key

7 Display of coordinates

8 Display of defined limits

Software

1 2

Tran sferMan

English (EN)

5.1.2 Display of coordinates

Abb. 5-2:Display of coordinates

Fig. 5-2: Display of coordinates

1 Motor limit stop

+ = positive, - = negative

2Axis is switched off

3Lower limit (Z-axis Limit) reached

4Display of lower limit

5 Downward limit is switched on

5.1.3 Menu display

Abb. 5-3:Menu and parameter display

Fig. 5-3: Menu and parameter display

Software

Tran sferMan

English (EN)

1 Navigation path

2Menu

3 Arrow up/down softkey

Navigation and changing parameters.

4 Enter softkey

For confirming input, executing the function, saving parameters

5 Arrow left/right softkey

Navigation

6 Parameter

Software

Tran sferMan

English (EN)

5.2 Applications

Abb. 5-4:Application screen

Fig. 5-4: Application screen

Application selection

• Select the application

• Store the main application

5.2.1 Application parameters

Predefined softkeys of the different applications.

• Pos 1 – Save the X, Y and Z values of the capillary position. Switch the position using the joystick key.

• Pos 2 – Save the X, Y and Z values of the capillary position. Switch the position using the joystick key.

• Pos 3 – Save the X, Y and Z values of the capillary position. Switch the position using the joystick key.

• Y off – Switch off the movement of the capillary in the Y-axis. Prevents moving sideways during injection.

• Z-axis Limit – Set the lower limit for vertical capillary movement.

• Axial – Switch on the capillary movement along the mounting bracket.

Software

Tran sferMan

English (EN)

5.2.2 Application – Cell transfer

This application is suitable for, e.g., stem cell transfer, polar body biopsy and similar applications.

Abb. 5-5:Application 1

Fig. 5-5: Application 1 Cell transfer

Parameter selection

• Double-click on joystick key – switch position

• Save positions 1, 2 and 3

• Freely program softkey

• Set lower limit (Z-axis Limit)

5.2.3 Application – ICSI

This application is suitable for intracytoplasmic sperm injection.

Abb. 5-6:Application 2

Fig. 5-6: Application 2 ICSI

Parameter selection

• Double-click on joystick key – switch position

• Save positions 1 and 2

• Freely program softkey

• Switch off control of movement axis (Y-axis)

• Set lower limit (Z-axis Limit)

Software

Tran sferMan

English (EN)

5.2.4 Application – DNA injection

This application is suitable for injection movements along the horizontal plane (e.g., pronucleus injection).

Abb. 5-7:Application 3

Fig. 5-7: Application 3 DNA injection

Parameter selection

• Double-click on joystick key – switch position

• Save positions 1 and 2

• Freely program softkey

• Switch off control of movement axis (Y-axis)

• Set lower limit (Z-axis Limit)

5.2.5 Application – Basic

This application is suitable for injection movements into larger organisms (e.g., Drosophila embryos).

Abb. 5-8:Application 4

Fig. 5-8: Application 4 Basic

Parameter selection

• Double-click on joystick key – switch position

• Save position 1

• Freely program softkey

• Switch on axial movement of Z-axis

• Freely program softkey

• Set lower limit (Z-axis Limit)

Software

Tran sferMan

English (EN)

5.2.6 Application – My application

For this application no softkeys are preprogrammed. The application can be individually programmed.

Abb. 5-9:Application 5

Fig. 5-9: Application 5 My application

Parameter selection

• Freely program joystick key

• Freely program all softkeys

5.3 Main menu

Abb. 5-10:Main menu

Fig. 5-10: Main menu

Menu Parameter

Speed Set the working range and the speed parameters

Step injection Set the injection parameters

Home Set the parameters for the Home movement

Clean Set the parameters for the Clean movement

PiezoXpert Set the parameters for an optional device

Installation Set the device parameters

Function Execute the device function

Softkeys Program the softkeys

Change appl. Change the selected application or activate the application screen

Service Execute the service function on a user basis

Software

Tran sferMan

English (EN)

5.4 Navigating the menu

Abb. 5-11:Software navigation

Fig. 5-11: Software navigation

Navigate the menu using the arrow keys. Pressing Enter will confirm the selection. You can switch between menus and submenus with the arrow keys to the left and to the right.

5.4.1 Entering or changing parameters

Parameters can be changed in the menu using the arrow keys, the selection dial or the rotating wheel on the joystick.

Abb. 5-12:Changing the parameters

 Change values with the upwards or

downwards arrow keys.

 Change values using the selection dial.  Change values using the upper part of

the joystick.

 Save with Enter.

Fig. 5-12: Changing the parameters

6Operation

WARNING! Electric shock due to damage to device or mains cable.

 Only switch on the device if the device and mains cable are undamaged.  Only use devices that have been properly installed or repaired.  In case of danger, disconnect the device from the mains supply by pulling the

power plug from the device or the mains socket or, by using the isolating device intended for this purpose (e.g., emergency stop switch in the laboratory).

Do not move the joystick immediately after power-on. Wait until the initialization is completed. The completion of initialization is indicated by the display switching to the operating state.

6.1 Switching the device on or off

6.1.1 Switching on the device

1. Switch on the device at the mains power switch.

• The motor module and the control board are switched on.

• The device runs through an initialization phase.

• The application screen will appear.

6.1.2 Switching the device off

1. Switch off the device at the mains power switch.

The motor module and the control board are deenergized.

Operation

Tran sferMan

English (EN)

6.2 Activating or deactivating the control board

6.2.1 Activating the control board

Prerequisites

• The display shows STANDBY.

1. Press the standby key.

• The keys, joystick, selection dial and softkeys are activated.

• The display shows the application screen.

6.2.2 Deactivating the control board

The step motors slowly mo ve to the next parking position. This prevents the motors falling back to the parking position and the capillary jumping.

1. Press the standby key.

• The keys, joystick and selection dial are deactivated

Operation

Tran sferMan

English (EN)

• The display shows STANDBY.

• Current movements are stopped.

• The motor module remains switched on so that the step motors keep their current position.

6.3 Defining the start screen

An application can be selected as the default application. The micromanipulator then starts with the defined application. The application screen with all the applications can be redefined in the Start display menu.

6.3.1 Defining the application

1. Press and hold the softkey of the desired application for 3 seconds.

• The micromanipulator starts with the defined application.

6.3.2 Defining the selected application

1. In the Change appl. menu, select the Start display submenu.

2. Confirm with Execute.

3. Close the menu.

• The micromanipulator starts with the selected application.

6.4 Replacing the capillary

Prerequisites

• The capillary is depressurized.

WARNING! Risk of injury due to flying capillaries and glass splinters.

If exposed to high pressures, capillaries may detach themselves from the grip heads and become projectiles. Capillaries can crack as a result of incorrect handling.

 Wear protective goggles.  Never aim capillaries at people.  Use capillaries with an outer diameter that matches the grip head

specifications.

 Always mount / dismount capillaries when they are depressurized.  Mount the capillary correctly in the grip head.  Do not touch the capillary with the Petri dish or other objects.

Operation

Tran sferMan

English (EN)

CAUTION! Risk of injury from capillaries

Capillaries can easily penetrate your skin.

 After changing a capillary, swivel it immediately back to the working range.

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

1. Move the capillary out of the working range using the home key.

2. Swing the X-module forwards.

3. Undo the grip head on the capillary holder.

4. Carefully pull the capillary out of the grip head.

5. Push the new capillary into the grip head until it reaches the stop and tighten the grip head.

6. Swing the X-module back.

6.4.1 Manually positioning the capillary

Suitable when using capillaries of different lengths (e.g., self-pulled capillary).

1. Press Back manual.

2. Manually position the capillary in the working range.

6.4.2 Automatically positioning the capillary

Suitable when using industrial capillaries (e.g., Femtotips).

1. Press home.

• The capillary automatically moves back into the working range.

Operation

Tran sferMan

English (EN)

6.5 Changing the sample

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

6.6 Changing the size of the working range

6.6.1 Change parameters using the selection dial

1. Press the home key to move the capillary out of the working range.

2. Swing the X-module backwards.

3. Change the sample.

4. Swing the X-module back.

5. Press the home key to move the capillary back into the working range.

coarse

1. Press the key for the required working range on the control panel.

2. Turn the selection dial to change the

fine

value of the working range.

Operation

Tran sferMan

English (EN)

6.6.2 Changing parameters in the menu

Prerequisites

• An application has been selected.

1. Press the menu key.

2. Select the Speed menu.

3. Select the desired parameter.

4. Change the value.

6.7 Shifting the movement range of the capillary

6.7.1 Extending the movement range into the dynamic range

When the movement range of the capillary is not sufficient, the joystick can be moved into the dynamic range. By doing that you can move the capillary further into the required direction.

1. Move the joystick into the dynamic range.

2. Keep the joystick in the dynamic range until the capillary has reached the desired position.

The further you move the joystick forwards or backwards, the faster the capillary will move.

6.7.2 Uncoupling and resetting the joystick

When the current movement range of the capillary is not in the correct position, the joystick movement can be uncoupled from the capillary movement. By doing that you can reset the joystick and move the capillary further into the required direction.

1. Hold the joystick button.

2. Move the joystick in the opposite direction.

3. Release the joystick.

The capillary can now be moved further into the required direction.

6.8 Capillary positions

To secure the capillary when moving a slide and to move back to the same work position you can store the positions. Depending on the application, a maximum of five positions can be stored.

As soon as the capillary leaves a stored position, the filled circle is displayed as an empty circle, to show that this position is now stored. If no positions are stored, the status field will be empty.

Operation

Tran sferMan

English (EN)

6.8.1 Saving a position

Prerequisites

• An application has been selected.

1. Move the capillary to the required position.

2. Hold the Pos 1 softkey for approx. one second to store the working position of the capillary.

• An acoustic signal will sound.

• Pos 1 is marked.

• The stored position is displayed in the status field.

3. Move the capillary to the desired position (e.g., parking position).

4. Press and hold the Pos 2 softkey for approx. one second to store the parking position of the capillary.

• An acoustic signal will sound.

• Pos 2 is marked.

• The stored position is displayed in the status field.

6.8.2 Move to the position using the softkey

Prerequisites

• At least one position is stored.

1. Press a softkey with a stored position.

• You will move to the selected position.

• The joystick is deactivated until the position has been reached.

• The LEDs flash.

• In the softkey status field a filled circle is displayed.

If a stored position is lower than the lower limit (Z-axis Limit), the position on the Z-axis will only be approached up to the defined limit.

Operation

Tran sferMan

English (EN)

6.8.3 Move to the position using the joystick button

Prerequisites

• At least one position is stored.

•The Joystick key parameter must be set to the Switch position value.

 Press the joystick key twice.

It will move to the first position

 Press the joystick key twice.

It will move to the next position

6.8.4 Deleting a stored position

Prerequisites

• A position is stored.

1. When the position has been reached, hold the softkey.

• An acoustic signal will sound.

• The position has been cleared.

• The status field is empty

6.8.5 Overwriting a stored position

Prerequisites

• A position is stored.

1. Press the softkey for a different position. It will move to the position.

2. When the position has been reached, press and hold the softkey for the position to be overwritten.

• The old position is overwritten with the current coordinates.

3. Press the softkey.

• An acoustic signal will sound.

• In the softkey status field a filled circle is displayed.

• The stored position is displayed in the coordinate field.

6.9 Vertical limits

For the Z-axis, a lower and upper limit can be defined. This prevents the capillary coming into contact with the bottom of the Petri dish or moving against the condenser of the microscope adapter.

•Lower limit – Z-axis Limit

• Upper limit – Upper limit

Operation

Upper Limit

Menu / Installation / Upper Limit

Back

Set

Clear

X Y Z

Lim

= = =

0 0

2671

µm µm µm U

µm

Tran sferMan

English (EN)

6.9.1 Defining the lower limit

Prerequisites

• An application has been selected.

1. Position the capillary a little way above the slide.

2. Press Z-axis Limit.

• The Z-coordinate is marked with L.

• The value for the limit (Limit Z) is displayed.

• Z-axis Limit is selected.

• The capillary cannot be moved any lower.

6.9.2 Deleting the lower limit

1. Press Z-axis Limit.

• The limit is deleted.

6.9.3 Defining the upper limit

1. In the Installation menu, select the Upper limit submenu. The Upper Limit window appears.

2. Move the capillary to the top position.

3. Save the position with Set.

• The Z-coordinate is marked with U.

• The value for the limit (Lim) is displayed.

• The capillary cannot be moved any higher.

Operation

Tran sferMan

English (EN)

6.9.4 Deleting the upper limit

1. In the Installation menu, select the Upper limit submenu. The Upper Limit window appears.

2. Save the position with Clear.

• The limit is deleted.

6.10 Horizontal limit

For the X-axis, a limit can be defined for a horizontal injection. This prevents the capillary from moving through the sample.

6.10.1 Defining the horizontal limit

1. In the Installation menu, select the Angle submenu.

2. Set the injection angle to 0° and save with Enter.

3. Close the menu. The application screen now displays X-axis Limit.

4. Move the capillary to the desired final position for the X-axis.

5. Save the lateral limit with X-axis Limit.

• The X-coordinate is marked with L.

• The value for the limit (Limit X) is displayed.

• X-axis Limit is selected.

• The capillary cannot be moved any further to the side.

6.10.2 Deleting the horizontal limit

1. Press X-axis Limit. The limit is deactivated.

2. In the Installation menu, select the Angle submenu.

3. Reset the injection angle to the operating angle and save with Enter.

• The lateral limit is deleted.

• The application screen displays Back again.

Operation

Tran sferMan

English (EN)

6.11 Speed function

In the Speed menu you can set the size of the working range and the speed at which certain positions are approached or movements executed.

6.11.1 Speed menu and parameters

Abb. 6-1:Menu –

Fig. 6-1: Menu – Speed

Parameter Value Range of values Increment Standard

Coarse Set size in μm 5 – 12500 5 6000

Fine Set size in μm 5 – 2000 5 250

X-fine Set size in μm 0 – 600 1 80

Step inj. speed Set speed in μm per second 5 – 10000 5 300

Position speed Set speed in μm per second 5 – 10000 5 1500

Home speed Set speed in μm per second 5 – 10000 5 7500

6.11.2 Setting the parameter for Speed

If a value of 0 is set for the parameter X-fine, then the option of changing between the Fine and X-fine working ranges is deactivated.

1. Select the desired parameter.

2. Set the value for the parameter.

3. Close the menu.

Operation

Tran sferMan

English (EN)

6.12 Step injection function

With this function you can execute a straight injection over a defined distance. Step

injection can only be executed when a FemtoJet is connected.

6.12.1 Step injection menu and parameters

Abb. 6-2:Menu –

Fig. 6-2: Menu – Step injection

Parameter Value Range of values Increment Standard

Step injection Switch function on or off OFF/ON – OFF

Step inj. speed Set inspection speed in μm per

5 – 10000 5 300

second

Step inj. dist. Set injection path in μm 1 – 2000 1 20

Step inj. axial Switch axial movement of the

OFF/ON – On

capillary on or off

Step inj. back Switch backward movement of

OFF/ON – On

the capillary on or off

Synchr. inject. Set the time of the injection MOVE

– LIMIT

IMMEDIATE LIMIT PRESSURE

Parameter Range of values Functi on

Synchr. inject. MOVE Injection movement without injection pressure

IMMEDIATE Injection pressure starts with injection movement

LIMIT Injection pressure at the end of the injection distance

(Step inj. dist.)

PRESSURE Injection pressure without injection movement

Operation

Tran sferMan

English (EN)

6.12.2 Executing the Step injection function

Prerequisites

• A FemtoJet is connected.

• Step injection is assigned to a free softkey.

1. Activate Step injection.

2. Set Step inj. dist.

3. Set the injection time on the FemtoJet.

4. Select Step inj. dist.

5. Close the menu.

• The application screen displays Step injection.

6. Trigger the injection by selecting Inject on the FemtoJet.

• The function is executed.

6.13 Home function

The Home function quickly moves the capillary out of the work area and is suitable for a quick change of capillary.

6.13.1 Home menu and parameters

Abb. 6-3:Menu –

Fig. 6-3: Menu – Home

Parameter Value Range of values Increment Standard

Home speed Set the speed of the Home

5 – 10000 5 7500

function

Home offset Set vertical offset in μm 5 – 20000 5 0

6.13.2 Setting the parameter for Home

1. Set the speed.

2. Set the offset.

3. Close the menu.

6.13.3 Move the capillary out with the home key

Prerequisites

• The parameters in the Home menu have been defined.

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

1. Press the home key. The home key flashes.

• The capillary is moved out of the work area.

•The home key lights up.

6.13.4 Move the capillary back with the home key

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

Operation

Tran sferMan

English (EN)

1. Press the home key.

• The capillary is moved back into the work area.

•The home function is terminated.

6.13.5 Setting the offset.

For capillaries of different lengths, an offset can be set. This defines a limit for the home function.

1. Change the offset using the arrow keys.

• The value for the offset is displayed in the status field.

Operation

Tran sferMan

English (EN)

6.13.6 Terminate the home function.

1. Press Back manual. The home function is terminated.

2. Move the capillary manually using the joystick.

6.14 Clean function

With this function you can move the capillary over a defined distance out of the medium. In the process, outside contamination is removed from the capillary at the medium boundary.

6.14.1 Clean menu and parameters

Abb. 6-4:Menu –

Fig. 6-4: Menu – Clean

Parameter Value Range of values Increment Standard

Home speed Set the speed of the clean

5 – 10000 5 7500

function

Clean distance Set distance in μm 0 – 20000 5 10000

6.14.2 Setting the parameter for Clean

Set the Clean distance above the medium boundary

1. Set Home speed.

2. Set Clean distance.

3. Close the menu.

6.14.3 Execute the Clean function

Prerequisites

• Parameters are set for Clean.

• Clean is assigned to a softkey.

1. Press Clean. The screen for Clean appears.

2. Execute the function with Clean.

• The capillary is moved out of the medium with the set parameters.

Operation

Tran sferMan

English (EN)

6.14.4 Terminate the Clean function.

• The function is terminated.

• The application screen appears.

1. Press Back manual.

Operation

Tran sferMan

English (EN)

6.15 PiezoXpert function

6.15.1 PiezoXpert menu and parameters

Abb. 6-5:Menu –

Fig. 6-5: Menu – PiezoXpert

Parameter Value Range of values Increment Standard

Synchr. PiezoXp.

Switch synchronization between TransferMan 4r and

OFF/ON

PiezoXpert on or off

PiezoXp. speed Set the advance rate of the

5 – 10000 5 300 manipulator in μm/s while the PiezoXpert triggers a pulse.

PiezoXp. dist. Set the length of the movement

1 – 2000 1 20 step of the manipulator in μm while the PiezoXpert triggers a pulse.

PiezoXp. axial Axial movement on or off

ON/OFF – OFF (corresponds to the set injection angle) On = movement in the X and Y-axis. Off = movement in the X-axis

Operation

Tran sferMan

English (EN)

6.15.2 Execute the PiezoXpert function

Prerequisites

• A PiezoXpert is connected.

1. Activate Synchr. PiezoXp.

2. Set PiezoXp. speed.

3. Set PiezoXp. dist.

4. Select PiezoXp. axial.

5. Close the menu.

6. Trigger the function with Channel A on the PiezoXpert.

• The function is executed.

6.16 Installation function

The function provides settings for making fine adjustments to the motor module and the control board. Parameters in the Installation menu override identical softkey functions.

6.16.1 Installation menu and parameters

Abb. 6-6:Menu –

Fig. 6-6: Menu – Installation

6.16.2 Installation parameters

Parameter Value Range of values Increment Standard

Side Set the mounting side LEFT/RIGHT – RIGHT

Angle Set the capillary angle in

0° – 90° 1 – degrees

Operation

Tran sferMan

English (EN)

6.16.3 Control board parameters

Parameter Value Range of values Increment Standard

LCD contrast Set the display contrast 65 % – 75 % 1 70 %

LCD light Set the brightness level of the

0 % – 100 % 1 100 % display

Beeper Set the volume 0 – 100 % 1 50 %

Display

Set the coordinate display OFF/ON – On

coordin.

6.16.4 Joystick parameters

Parameter Value Range of values Increment Standard

Z Factor Acceleration factor for the

5 % – 400 % 5 100 Z-axis relative to the X and Y-axis

Dyn-mode Switch the dynamic movement

OFF/ON – On range on or off

Dyn-factor Set the dynamic factor 0 % – 1000 % 1 100 %

6.16.5 Module parameters

Parameter Value Range of values Increment Standard

Invert X-axis Reverse the direction of

OFF/ON – OFF movement of the motor

Invert Y-axis Reverse the direction of

OFF/ON – OFF movement of the motor

Invert Z-axis Reverse the direction of

OFF/ON – OFF movement of the motor

X-axis off Switch the motor of the

OFF/ON – OFF movement axis on or off

Y-axis off Switch the motor of the

OFF/ON – OFF movement axis on or off

Z-axis off Switch the motor of the

OFF/ON – OFF movement axis on or off

Upper limit Define the upper limit Execute – –

Operation

Tran sferMan

English (EN)

6.17 Function function

Function allows you to reset the parameters and coordinates and to move the modules to

the middle position.

6.17.1 Function menu and parameters

Abb. 6-7:Menu –

Fig. 6-7: Menu – Function

Parameter Value Range of values

Zero coordin. Reset all coordinates to zero Execute

Center motors Move step motors of the motor module to the middle

Execute

position

User default Reset the settings to the delivery status Execute

6.17.2 Executing Zero coordin.

1. Select the parameters and confirm with Enter.

2. Confirm Execute with Enter.

• All coordinates are reset to zero.

• Saved positions are deleted.

• Set limits are deleted.

6.17.3 Executing Center motors

Prerequisites

• No capillary holder is clamped.

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

1. Select the parameters and confirm with Enter.

2. Confirm Execute with Enter.

• X-motor and Y-motor are moved to the middle position.

Operation

Tran sferMan

English (EN)

• Z-motor is moved to a 20/80 position.

• All coordinates are reset to zero.

• Saved positions are deleted.

• Set limits are deleted.

6.17.4 Executing User default

1. Select the parameters and confirm with Enter.

2. Confirm Execute with Enter.

• All parameters are reset to the factory settings.

• The application screen appears.

6.18 Softkeys function

With this function you can assign programs to free softkeys. Assigned softkeys are indicated with a lock symbol.

6.18.1 Softkeys menu and parameters

Abb. 6-8:Menu –

Fig. 6-8: Menu – Softkeys

Parameter Value Range of values

Softkey 1 Set the function

Softkey 2 Set the function

Softkey 3 Set the function

Softkey 4 Set the function

Softkey 5 Set the function

Joystick key Set the function for the joystick key No function

Switch position Switch fine

Operation

Tran sferMan

English (EN)

Parameter Function

No function Softkey has no function

Pos 1 Save, overwrite or delete a position

Pos 2 Save, overwrite or delete a position

Pos 3 Save, overwrite or delete a position

Pos 4 Save, overwrite or delete a position

Pos 5 Save, overwrite or delete a position

Y-axis off Switch the control of the movement axis on or off

Axial Change vertical movement to axial movement

Limit Activate or deactivate the vertical or horizontal (X-axis) limit

Step injection Activate the Step injection function

Limit up Amend value for Z-axis Limit upwards

Limit down Amend value for Z-axis Limit downwards

Clean Axial movement out of the working range

Z-axis only Only control of movements in the Z-axis. The control of movements in the

X and Y-axis is disabled

LCD light Set the brightness level of the display

Beeper Set the volume

Joystick off Deactivates all joystick movements

X-axis off Switch the control of the movement axis on or off

Z-axis off Switch the control of the movement axis on or off

X-axis only Only control of movements in the X-axis. The control of movements in the

Y and Z-axis is deactivated

Y-axis only Only control of movements in the Y-axis. The control of movements in the

X and Z-axis is deactivated

Dyn-mode Activate or deactivate dynamic movement range

Parameter Range of values Function

Joystick key No function Deactivate the function of the joystick key

Switch position Double-click to go to the next saved position

Switch fine Double-click to go to the fine or x-fine working range

Operation

Tran sferMan

English (EN)

6.18.2 Execute Softkeys

1. Select the free softkey and confirm with Enter.

2. Select the desired parameter and confirm with Enter.

3. Close the menu.

• The selected parameter is assigned to the free softkey.

• The parameter appears on the application screen.

6.18.3 Execute Joystick key

Prerequisites The My application application has been selected.

1. Select Joystick key and confirm with Enter.

2. Select the desired function and confirm with Enter.

3. Close the menu.

• The function is assigned to the joystick key.

• The selected function appears on the application screen.

6.19 Change appl. function

With this function you can define each application as the start screen or reactivate the default screen.

6.19.1 Change appl. menu and parameters

Abb. 6-9:Menu –

Fig. 6-9: Menu – Change appl.

Parameter Value Range of values

Start display Reactivate start screen to select an application Execute

Application Define the selected application as the start screen Cell transfer

ICSI DNA injection Basic My application

6.19.2 Defining the selected application as the start screen

1. Select Start display.

2. Confirm with Execute.

3. Close the menu.

• The micromanipulator starts with the selected application.

6.19.3 Defining the application as the start screen

1. Select the application.

2. Confirm with Enter.

3. Close the menu.

• The micromanipulator always starts with the defined application.

6.20 Service function

6.20.1 Service menu and parameters

Abb. 6-10:Service

Operation

Tran sferMan

English (EN)

Fig. 6-10: Service

Parameter Value Range of values

Selftest Move the motor module in all three axes and output

Execute

error message

Device info Display software version Execute

Set clock Set date and time YYYY-MM-DD

00:00

Movements Show movement routes of the modules (X, Y, Z) Execute

Show errors Display last 10 error messages Execute

Demo run Save the position and move to it Execute

Operation

Tran sferMan

English (EN)

6.20.2 Execute the Selftest function

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

1. Select Selftest and confirm with Enter.

2. Confirm Execute with Enter. The screen for the Selftest function appears.

3. Execute Selftest with Start. The motors move up to the limit stops.

4. Terminate Selftest with Stop.

6.21 Resetting parameters to the factory settings

6.21.1 Perform reset

Reset all parameters to the factory settings.

1. Keep the home key pressed.

2. Switch on the device at the mains power switch. The screen for GENERAL RESET appears.

3. Execute the function with Yes.

• All parameters are now reset to the factory settings.

6.21.2 Carrying out a reset in the menu

Prerequisites

• An application has been selected.

1. Press the menu key.

2. Select the Function menu.

3. Press Enter.

4. Select User default and confirm with Execute.

• All parameters are now reset to the factory settings.

6.22 Remote computer control of the TransferMan 4r

You can control the TransferMan 4r by means of a terminal program remotely via computer. Computer control by means of a terminal program is only recommended for certain applications and is not described in this operating manual. A special operating manual for this functionality is available at the following Internet address: www.eppendorf.com

Eppendorf does not provide any support for controlling the TransferMan 4r by means of a terminal program.

7Troubleshooting

7.1 General errors

7.1.1 Motor module

Symptom/message Cause Remedy

Motor axes move in the wrong direction or do not match joystick movement.

• Motor module mounted incorrectly.

• Incorrect installation parameters entered.

• Direction of movement of axis inverted.

 Compare the installation

parameters with the structure of the motor module.

 Check the orientation and

structure of the modules.

 Check the connections of the

modules at the control board.

 Cancel the inversion of the

axis.

7.1.2 Capillary

Symptom/message Cause Remedy

Capillary moves too fast or too slow.

Capillary only moves to the side or vertically.

Capillary does not move down far enough.

• Radius of working range incorrectly set.

• Incorrect acceleration factor.

•Y-axis is disabled.

•The Z-axis Limit function is active.

• Capillary is incorrectly adjusted.

 Set the radius using the

selection dial or in the Speed menu.

 In the Installation menu, set

the value for the Dyn-factor parameter.

 The Y off function disabled.

 Deactivate the Z-axis Limit

function.

 Readjust the capillary.

Troubleshooting

Tran sferMan

English (EN)

7.1.3 Control board and display

Symptom/message Cause Remedy

Device does not respond to keystroke when Home function is active.

• Function is active.

 Press the home key again.

The capillary moves down.

 Press the Back manual

softkey.

 Move the joystick.

Troublesho oting

Tran sferMan

English (EN)

Symptom/message Cause Remedy

The display does not show anything or the device cannot be activated although the device is connected.

• The power cable or power plug is loose.

• The device is switched off.

7.1.4 Joystick

Symptom/message Cause Remedy

Outer movement range of the joystick does not work.

• Outer movement range is deactivated.

7.1.5 Software and parameters

Symptom/message Cause Remedy

Parameters are not accessible for certain operations.

–

 Check the power cable or

the power plug.

 Switch the device on.

 In the Installation menu, set

the value for Dyn-mode to

ON.

 Enter parameters again.  Carry out a reset and reset

all parameters to the works settings.

 Readjust device.

7.2 Error messages

7.2.1 Warnings

Symptom/message Cause Remedy

WARNING 1 • FemtoJet is not connected.

 Disconnect the data cable to

the FemtoJet and connect it again.

 Switch on the FemtoJet.

WARNING 3 • X-module is not connected.

 Switch off the device.  Connect the plug of the

X-module to the control board and tighten.

 Switch the device on.

WARNING 4 • Y-module is not connected.

 Switch off the device.  Connect the plug of the

Y-module to the control board and tighten.

 Switch the device on.

Troubleshooting

Tran sferMan

English (EN)

Symptom/message Cause Remedy

WARNING 5 • Z-module is not connected.  Switch off the device.

 Connect the plug of the

Z-module to the control board and tighten.

 Switch the device on.

WARNING 6 • Synchronization error with

FemtoJet during an injection.

7.2.2 Error

Symptom/message Cause Remedy

ERROR 10 – ERROR 99 • Technical error.

 Set another synchronization

for Synchr. inject..

 Set the synchronization

IMMEDIATE, LIMIT or PRESSURE.

 Switch the device off and

back on.

 Reset the parameters to the

factory settings.

 Contact Eppendorf Service.

Maintenance

Tran sferMan

English (EN)

8 Maintenance

8.1 Replacing fuses

DANGER! Electric shock.

 Switch off the device and disconnect the power plug before starting

maintenance or cleaning work.

The fuse holder is located between the mains connection socket and the mains power switch. The fuses may only be replaced with the same type of fuse.

1. Disconnect the mains plug.

2. Pull the fuse holder out completely.

3. Replace the defective fuse.

4. Insert the fuse holder.

8.2 Cleaning

DANGER! Electric shock as a result of penetration of liquid.

 Switch off the device and disconnect the power plug before starting cleaning

or disinfection work.

 Do not allow any liquids to penetrate the inside of the housing.  Do not spray clean/spray disinfect the housing.  Only plug the device back in if it is completely dry, both inside and outside.

NOTICE! Damage from the use of aggressive chemicals.

 Do not use any aggressive chemicals on the device or its accessories, such as

strong and weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.

 If the device has been contaminated by aggressive chemicals, immediately

clean it by means of a mild cleaning agent.

Prerequisites

• Clean at least every 4 weeks.

1. Wipe the painted parts and the aluminum surfaces with a cloth and mild detergent.

2. Wipe with a dry cloth afterwards.

Maintenance

Tran sferMan

English (EN)

8.3 Service and maintenance

Application-specific service and safety inspections are not required

The Eppendorf AG service team is available to service and certify your device.

Service provisions:

•Service

• Operational qualification (OQ) according to manufacturer's specifications

• Electrical safety testing according to country-specific regulations

•Software update

Information on the services offered can be found on our webpage: www.eppendorf.com/ epservices.

8.4 Disinfection/decontamination

Choose the disinfection method which corresponds to the legal regulations and guidelines in place for your range of application. If you have any questions on cleaning, disinfection, decontamination, or the cleaning agents to be used, please contact Eppendorf AG Application Support. The relevant contact details are provided on the back of this operating manual.

Prerequisites

• All device parts are cleaned.

• A disinfectant containing alcohol (such as isopropanol or spirit) is available

1. Wipe all parts of the device with a cloth and the disinfectant.

Technical data

Tran sferMan

English (EN)

9 Technical data

Motor module

Travel, maximum 20 mm

Step motors X-module, Y-module, Z-module

Weight 2150 g

Module (X,Y,Z)

Type Step motors

Step size

< 20 nm

(calculated resolution)

Speed, maximum 10000 μm/s

Width 129 mm

Depth 51 mm

Height 36 mm

Weight 570 g

Swivel joint

Direction of rotation -45° – +90°

Capillary change Direction of rotation forwards

Sample change Direction of rotation backwards

Angle head

Operating angle 0° – 90°

Control board

Control Joystick

Working range coarse, fine, x-fine

Width 205 mm

Depth 288 mm

Height 152 mm

Weight 1800 g

9.1 Power supply

Control board

Mains connection 120 – 240 V AC, 50 – 60 Hz

Power consumption 30 W

Protection class I

Overvoltage category II (IEC 61010-1)

Micro fuse T 1.6 A, 250 V

9.2 Interfaces

Module (X, Y, Z) SubD25, female

PC/external device Serial interface

SubD9, male

Service connection USB

9.3 Ambient conditions

Environment For indoor use only.

Ambient temperature 15 °C – 35 °C

Max. relative humidity 30 % – 65 %

Degree of pollution 2 (IEC 664)

Technical data

Tran sferMan

English (EN)

Transport, storage and disposal

Tran sferMan

English (EN)

10 Transport, storage and disposal

10.1 Storage

Air temperature Relative humidity Atmospheric

pressure

In transport packaging

Without transport packaging

-25 °C – 55 °C 10 % – 95 % 70 kPa – 106 kPa

-5 °C – 45 °C 10 % – 95 % 70 kPa – 106 kPa

10.2 Decontamination before shipment

If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following:

WARNING! Risk to health from contaminated device

 Please observe the information in the decontamination certificate, which is

available as a PDF file on our webpage (www.eppendorf.com/ decontamination).

 Decontaminate all the parts you would like to dispatch.  Include the fully completed decontamination certificate in the package.

10.3 Transport

NOTICE! Damage to the control board as a result of incorrect handling.

 Grasp the control board on the housing.  Do not lift the control board using the joystick.  Never place the control board on the joystick.

Air temperature Rel. humidity Atmospheric pressure

General transport -25 °C – 60 °C 10 % – 95 % 30 kPa – 106 kPa

Air freight -40 °C – 55°C 10 % – 95 % 30 kPa – 106 kPa

Carry out the following steps before transport:

1. Dismantle the module unit before transport.

2. Attach the supplied transport securing devices according to the red supplement sheet.

3. Only use original packaging for transport.

Transport, storage and disposal

Tran sferMan

English (EN)

10.4 Disposal

In case the product is to be disposed of, the relevant legal regulations are to be observed.

Information on the disposal of electrical and electronic devices in the European Community:

Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, in the business-to-business sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. To document this, they have been marked with the following identification:

Because disposal regulations may differ from one country to another within the EU, please contact your supplier if necessary.

In Germany, this is mandatory from March 23, 2006. From this date, the manufacturer has to offer a suitable method of return for all devices supplied after August 13, 2005. For all devices supplied before August 13, 2005, the last user is responsible for the correct disposal.

Ordering Information

Tran sferMan

English (EN)

11 Ordering Information

11.1 TransferMan 4r

Order no. (International)

Order no. (North America)

Description

TransferMan 4r

5193 000.012 5193 000.020 5193 000.039 5193 000.047 5193 000.055 5193 000.063

5193000012 5193000020 5193000039 5193000047 5193000055 5193000063

Mains/Power plug Europe Mains/Power plug USA/Japan Mains/Power plug UK/Hong Kong Mains/Power plug Australia Mains/Power plug China Mains/Power plug Argentina

11.2 Accessories for TransferMan 4r

Order no. (International)

Order no. (North America)

Description

5181 070.015 920005845 Data cable

Connect FemtoJet/FemtoJet express to micromanipulator

5181 150.051 920005799 Foot control

Data cable 5181

5181 150.094 920005837

Connect PC or PiezoXpert to micromanipulator

Antivibration Pad

5181 301.009 5181 303.001 5181 305.004 5181 307.007 5181 309.000

920007945 920007953 920007961 920007970 920007988

XS S M L XL

Spare parts kit

1 slider (complete), 2 machine screws M2.5×6 (swivel joint), 2 set screws (angle head), 2

5192 071.005 5192071005

compression springs (knurled screw angle head)

Positio ning ai d

2 pieces

5192 072.001 5192072001

for universal capillary holder

11.3 Tools for TransferMan 4r

Ordering Information

Tran sferMan

English (EN)

Order no. (International)

Order no. (North America)

Description

5192 074.004 5192074004 Allen torque screwdriver

3 mm

5192 075.000 5192075000 Allen screwdriver

1.3 mm

Allen key

7 pieces

5192 076.007 5192076007

1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6mm

5192 077.003 5192077003 Toolbag

11.4 Microscope adapter

Order no. (International)

Order no. (North America)

Description

Adapter for microscope

Leica 1 DMI3000 B, 3000 M, 4000 B, 5000 B, 5000 M,

5192 301.000 5192301000

6000 B, DM IRB E, HC, DM IRE 2

Adapter for microscope

Leica 2

5192 302.007 5192302007

DM IL LED, HC

Adapter for microscope

Olympus 1

5192 306.002 5192306002

IX50, IX51, IX70, IX80, IX81

Adapter for microscope

Olympus 2

5192 307.009 5192307009

IX53, IX73

Adapter for microscope

Nikon 1 Eclipse® Diaphot 200, 300, Eclipse® Ti-E, Ti-U,

5192 316.008 5192316008

Ti-S, TE200, TE300, TE2000

Adapter for microscope

Zeiss 1

5192 311.006 5192311006

Axiovert® 100, AxioObserver A1, D1, Z1

Adapter for microscope

Zeiss 2

5192 312.002 5192312002

Axio Vert.A1

Ordering Information

Tran sferMan

English (EN)

11.5 Accessories for adapters for microscope

Order no. (International)

Order no. (North America)

Description

Universal stand

for micromanipulators

5192 325.007 5192325007

TransferMan 4m/4r, InjectMan 4

Adapter bridge

5192 321.001 5192321001

TransferMan 4m/4r, InjectMan 4

11.6 Capillaries for research applications

Order no. (International)

Order no. (North America)

Description

epT.I.P.S. Racks, Microloader

Eppendorf Quality, 2 racks of 96 tips

5242 956.003 930001007

0.5 - 20 μL, light gray, length: 100 mm

Femtotips

5242 952.008 930000035

20 pieces

Femtotips II

5242 957.000 930000043

20 pieces

TransferTip (ES)

5175 107.004 930001040

25 pieces, sterile

Piezo Drill Tip

for mouse ICSI

5175 220.005 930001091

25° angle, 6 mm rigid flange, sterile, set of 25

Piezo Drill Tip (ES)

for transfer of ES cells into blastocysts 25° angle, 6 mm rigid flange, 15 μm inner

5175 250.001 930001104

diameter, sterile, set of 25

11.7 Capillaries

Products are registered as a medical device in Europe (according to the Medical Device Directive (MDD) 93/42/EEC).

Order no. (International)

Order no. (North America)

Description

VacuTi p

5175 108.000 930001015

25 pieces, sterile

Ordering Information

Tran sferMan

English (EN)

Order no. (International)

Order no. (North America)

Description

VacuTi ps FCH

5175 240.006 5175240006

25 pieces

TransferTip-RP (ICSI)

5175 114.000 930001074

25 pieces, sterile

TransferTip-F ( ICSI)

5175 106.008 930001031

25 pieces, sterile

TransferTip-R (ICSI)

5175 113.004 930001066

25 pieces, sterile

Polar Body Biopsy Tip MML

5175 210.000 5175210000

25 pieces

Polar Body Biopsy Tip FCH

5175 230.000 5175230000

25 pieces

IMSI/TESE Tip

5175 117.000 5175117000

25 pieces, sterile

11.8 Universal capillary holder and accessories

Order no. (International)

Order no. (North America)

Description

Universal capillary holder

For holding Femtotips, Femtotips II or grip heads Including grip head 0, adapter for Femtotips and

5176 190.002 920007392

spare O-rings

Grip head 0

For microcapillaries with an outer diameter of

5176 210.003 920007414

1.0 mm to 1.1 mm, set of 2

Grip head 1

For microcapillaries with an outer diameter of

5176 212.006 920007708

1.2 mm to 1.3 mm, set of 2

Grip head 2

For microcapillaries with an outer diameter of

5176 214.009 920007716

1.4 mm to 1.5 mm, set of 2

Grip head 3

For microcapillaries with an outer diameter of

5176 207.002 920007406

0.7 mm to 0.9 mm, set of 2

O-ring set

5176 196.000 920005870

For grip heads 0-3 with removal tool

Ordering Information

Tran sferMan

English (EN)

Order no. (International)

Order no. (North America)

Description

Service kit

For grip heads and capillary holder

5176 195.004 920005888

Consisting of O-ring set, adapter for Femtotips

11.9 CellTram and accessories

Products are registered as a medical device in Europe (according to the Medical Device Directive (MDD) 93/42/EEC).

Order no. (International)

Order no. (North America)

Description

5176 000.017 920002021 CellTram Air

5176 000.025 920002030 CellTram Oil

5176 000.033 920002111 CellTram vario

Pressure tube

5176 114.004 920002081

1 m

11.10 FemtoJet

Order no. (International)

Order no. (North America)

Description

FemtoJet

programmable micro-injector with integrated

pressure supply 5247 000.013 5247 000.021 5247 000.030 5247 000.048 5247 000.056 5247 000.072 5247 000.080

5247000013 920010504 5247000030 5247000048 5247000056 5247000072 5247000080

Mains/Power plug Europe

Mains/Power plug Canada

Mains/Power plug UK / Hong Kong

Mains/Power plug Japan

Mains/Power plug South America

Mains/Power plug Australia

Mains/Power plug China

11.11 FemtoJet express

Ordering Information

Tran sferMan

English (EN)

Order no. (International)

Order no. (North America)

Description

FemtoJet express

programmable micro-injector 5248 000.017 5248 000.033 5248 000.041 5248 000.050 5248 000.076 5248 000.084

5248000017 5248000033 5248000041 920010521 5248000076 5248000084

Mains/Power plug Europe

Mains/Power plug UK / Hongkong

Mains/Power plug Japan

Mains/Power plug USA and Canada

Mains/Power plug Australia

Mains/Power plug China

11.12 Accessories for FemtoJet/FemtoJet express

Order no. (International)

Order no. (North America)

Description

Hand control

for remote-controlling the FemtoJet / FemtoJet 5252 076.010 5252076010

express

5181 150.051 920005799 Foot control

5181 070.015 920005845 Data cable

Connect FemtoJet/FemtoJet express to

micromanipulator

Pressure tube

for connecting the FemtoJet express to an external

pressure supply

Length 2.5 m, incl. 2 couplings G 1/4 inch and 1/ 5248 200.008 –

4 inch 18 NPT

Injection tubing

5246 164.004 920007431

2 m, for universal capillary holder

Ordering Information

Tran sferMan

English (EN)

11.13 PiezoXpert

Order no. (International)

5194 000.016 5194 000.024 5194 000.032 5194 000.059 5194 000.067 5194 000.075

Order no. (North America)

– 5194000024 5194000032 5194000059 5194000067 5194000075

Description

Eppendorf PiezoXpert

for piezo-assisted micromanipulation

with mains/power plug EU

with mains/power plug USA/Japan

with mains/power plug UK/Hong Kong

with mains/power plug Australia

with mains/power plug China

with mains/power plug Argentina

Tran sferMan

English (EN)

Index

Ambient conditions ................................ 85

Application screen.................................. 50

Cable....................................................... 40

Capillary

Replace ............................................. 56

Cleaning ................................................. 82

Connection

External device ................................. 45

Decontamination .................................... 86

Disinfectant ............................................ 83

Display

Coordinates ...................................... 48

Menu ................................................ 49

Parameter ......................................... 49

Disposal .................................................. 87

First set-up ............................................. 41

Function

Change appl...................................... 76

Clean................................................. 68

Function ............................................ 73

Home ................................................ 66

Installation ........................................ 71

Service............................................... 77

Softkeys............................................. 74

Speed ................................................ 64

Step injection..................................... 65

Initialization............................................ 55

Installation

First set-up ....................................... 41

Installation parameters .....................41

Selecting the location........................22

Limit

Horizontal limit .................................63

Lower limit ........................................62

Upper limit ........................................62

Vertical limit......................................61

X-limit ...............................................63

Z-Limit.........................................62, 62

Main menu ..............................................53

Overwriting

Delete ................................................61

positions......................................60, 61

Save...................................................60

Parameter input ...................................... 54

PC control

Terminal program .............................78

Power supply

Control board ....................................83

Reset .......................................................78

Sample

Change ..............................................58

Selecting the location..............................22

Service

Safety inspections .............................83

Service provisions .............................83

Software navigation ................................54

Standby ...................................................55

Index

Tran sferMan

English (EN)

Storage ................................................... 86

Switching off .......................................... 55

Switching on........................................... 55

Unpacking .............................................. 22

Upper Limit ............................................ 62

X-limit..................................................... 63

Z limit ..................................................... 62

Eppendorf TransferMan4 r User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

TransferMan® 4r

Table of contents

1 Operating instructions

1.1 Using this manual

1.2 Danger symbols and danger levels

1.2.1 Danger symbols

1.2.2 Danger levels

1.3 Symbols used

2 Product description

2.1 Delivery package

2.1.1 Tools

2.1.2 Accessories

2.2 Features

2.3 Main illustration

2.3.1 Motor module

2.3.2 Control board

2.4 Control panel

2.5 Joystick

2.5.1 Proportional range

2.5.2 Dynamic range

2.5.3 Direction of movement of the joystick

2.5.4 Direction of movement of the rotating wheel

2.5.5 Joystick key functions

2.6 Working range

2.7 Resulting speed

3Safety

3.1 Intended use

3.2 Warnings for intended use

3.3 Warning signs on the device

3.4 Information on product liability

4Installation

4.1 Preparing installation

4.1.1 If there is any damage, make a claim

4.1.2 Delivery incomplete

4.1.3 Microscope adapter assembly

4.2 Selecting the location

4.3 Mounting overview

4.3.1 Module (X, Y, Z)

4.3.2 Z-module holder

4.3.3 Slider

4.3.4 Angle head

4.3.5 Swivel joint

4.4 Mounting the motor module

4.4.1 Mounting the Z-module

4.4.2 Mounting the Y-module

4.4.3 Mounting the X-module

4.4.4 Mounting the angle head

4.5 Inserting the capillary holder into the angle head

4.6 Inserting the capillary into the capillary holder

4.6.1 Inserting Femtotips

4.7 Setting the injection angle

4.8 Aligning the motor module

4.8.1 Aligning the height

4.8.2 Aligning the depth

4.8.3 Aligning the width

4.8.4 Aligning the angle head

4.9 Entering mounting parameters

4.9.1 Microscope and adapter

4.9.2 Motor module

4.9.3 Angle head

4.10 Converting the swivel joint for left side mounting

4.11 Converting the angle head for left side mounting

4.12 Connect the motor module to the control board

4.13 Setting installation parameters

4.13.1 First set-up wizard

4.14 Connecting an external device

4.14.1 Connecting the FemtoJet

4.14.2 Connecting the PiezoXpert

4.14.3 Connecting the computer

4.14.4 Connecting two devices

5 Software

5.1 Display

5.1.1 Application display

5.1.2 Display of coordinates

5.1.3 Menu display

5.2 Applications