Eppendorf TransferMan4 r User Manual

www.eppendorf.com/myeppendorf

TransferMan® 4r

Operating manual

Eppendorf

and the Eppendorf logo are registered trademarks of Eppendorf AG,

Hamburg, Germany.

Tran sferMan

4r, FemtoJet® and Eppendorf PiezoXpert® are registered trademarks of

Eppendorf AG, Hamburg, Germany.

Registered trademarks are not marked in all cases with ™ or ® in this manual.

No part of this publication may be reproduced without the prior permission of the copyright owner.

5193 900.013-00/072013

Tran sferMan

English (EN)

Operating manualTransferMan® 4 rseeon p.Fig.Tab.p.

English (EN)Operating manual

Table of contents

1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.3.1 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.3.2 Control board. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.4 Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

2.5 Joystick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.5.1 Proportional range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.5.2 Dynamic range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.5.3 Direction of movement of the joystick. . . . . . . . . . . . . . . . . . . . . . 16

2.5.4 Direction of movement of the rotating wheel . . . . . . . . . . . . . . . . 17

2.5.5 Joystick key functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

2.6 Working range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

2.7 Resulting speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.3 Warning signs on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.1.1 If there is any damage, make a claim . . . . . . . . . . . . . . . . . . . . . . 22

4.1.2 Delivery incomplete . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.1.3 Microscope adapter assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.3 Mounting overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.3.1 Module (X, Y, Z). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

4.3.2 Z-module holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4.3.3 Slider . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4.3.4 Angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

4.3.5 Swivel joint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Table of contents

Tran sferMan

English (EN)

4.4 Mounting the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

4.4.1 Mounting the Z-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

4.4.2 Mounting the Y-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

4.4.3 Mounting the X-module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

4.4.4 Mounting the angle head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

4.5 Inserting the capillary holder into the angle head . . . . . . . . . . . . . . . . . . . . 31

4.6 Inserting the capillary into the capillary holder . . . . . . . . . . . . . . . . . . . . . . 31

4.6.1 Inserting Femtotips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

4.7 Setting the injection angle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

4.8 Aligning the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

4.8.1 Aligning the height . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

4.8.2 Aligning the depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

4.8.3 Aligning the width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

4.8.4 Aligning the angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

4.9 Entering mounting parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

4.9.1 Microscope and adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

4.9.2 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

4.9.3 Angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

4.10 Converting the swivel joint for left side mounting . . . . . . . . . . . . . . . . . . . . 36

4.11 Converting the angle head for left side mounting . . . . . . . . . . . . . . . . . . . . 39

4.12 Connect the motor module to the control board. . . . . . . . . . . . . . . . . . . . . . 40

4.13 Setting installation parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

4.13.1 First set-up wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

4.14 Connecting an external device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

4.14.1 Connecting the FemtoJet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

4.14.2 Connecting the PiezoXpert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

4.14.3 Connecting the computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

4.14.4 Connecting two devices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.1 Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.1.1 Application display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.1.2 Display of coordinates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

5.1.3 Menu display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

5.2 Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.2.1 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.2.2 Application – Cell transfer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.2.3 Application – ICSI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.2.4 Application – DNA injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

5.2.5 Application – Basic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

5.2.6 Application – My application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

5.3 Main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

5.4 Navigating the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

5.4.1 Entering or changing parameters . . . . . . . . . . . . . . . . . . . . . . . . . 54

Table of contents

Tran sferMan

English (EN)

6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.1 Switching the device on or off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.1.1 Switching on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.1.2 Switching the device off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.2 Activating or deactivating the control board. . . . . . . . . . . . . . . . . . . . . . . . . 55

6.2.1 Activating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.2.2 Deactivating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.3 Defining the start screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.3.1 Defining the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.3.2 Defining the selected application . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.4 Replacing the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.4.1 Manually positioning the capillary . . . . . . . . . . . . . . . . . . . . . . . . 57

6.4.2 Automatically positioning the capillary . . . . . . . . . . . . . . . . . . . . . 57

6.5 Changing the sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

6.6 Changing the size of the working range . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

6.6.1 Change parameters using the selection dial . . . . . . . . . . . . . . . . . 58

6.6.2 Changing parameters in the menu . . . . . . . . . . . . . . . . . . . . . . . . 59

6.7 Shifting the movement range of the capillary. . . . . . . . . . . . . . . . . . . . . . . . 59

6.7.1 Extending the movement range into the dynamic range . . . . . . . 59

6.7.2 Uncoupling and resetting the joystick. . . . . . . . . . . . . . . . . . . . . . 59

6.8 Capillary positions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

6.8.1 Saving a position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

6.8.2 Move to the position using the softkey . . . . . . . . . . . . . . . . . . . . . 60

6.8.3 Move to the position using the joystick button . . . . . . . . . . . . . . . 61

6.8.4 Deleting a stored position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.8.5 Overwriting a stored position . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.9 Vertical limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.9.1 Defining the lower limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.9.2 Deleting the lower limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.9.3 Defining the upper limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.9.4 Deleting the upper limit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

6.10 Horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

6.10.1 Defining the horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

6.10.2 Deleting the horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

6.11 Speed function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

6.11.1 Speed menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

6.11.2 Setting the parameter for Speed . . . . . . . . . . . . . . . . . . . . . . . . . . 64

6.12 Step injection function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

6.12.1 Step injection menu and parameters. . . . . . . . . . . . . . . . . . . . . . . 65

6.12.2 Executing the Step injection function . . . . . . . . . . . . . . . . . . . . . . 66

Table of contents

Tran sferMan

English (EN)

6.13 Home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.13.1 Home menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.13.2 Setting the parameter for Home . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.13.3 Move the capillary out with the home key . . . . . . . . . . . . . . . . . . 67

6.13.4 Move the capillary back with the home key . . . . . . . . . . . . . . . . . 67

6.13.5 Setting the offset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

6.13.6 Terminate the home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

6.14 Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

6.14.1 Clean menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

6.14.2 Setting the parameter for Clean . . . . . . . . . . . . . . . . . . . . . . . . . . 69

6.14.3 Execute the Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

6.14.4 Terminate the Clean function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

6.15 PiezoXpert function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

6.15.1 PiezoXpert menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . 70

6.15.2 Execute the PiezoXpert function . . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16 Installation function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16.1 Installation menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16.2 Installation parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16.3 Control board parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

6.16.4 Joystick parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

6.16.5 Module parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

6.17 Function function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.17.1 Function menu and parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.17.2 Executing Zero coordin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.17.3 Executing Center motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.17.4 Executing User default . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

6.18 Softkeys function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

6.18.1 Softkeys menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

6.18.2 Execute Softkeys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

6.18.3 Execute Joystick key . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

6.19 Change appl. function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

6.19.1 Change appl. menu and parameters . . . . . . . . . . . . . . . . . . . . . . . 76

6.19.2 Defining the selected application as the start screen . . . . . . . . . . 77

6.19.3 Defining the application as the start screen . . . . . . . . . . . . . . . . . 77

6.20 Service function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

6.20.1 Service menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

6.20.2 Execute the Selftest function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

6.21 Resetting parameters to the factory settings . . . . . . . . . . . . . . . . . . . . . . . . 78

6.21.1 Perform reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

6.21.2 Carrying out a reset in the menu . . . . . . . . . . . . . . . . . . . . . . . . . . 78

6.22 Remote computer control of the TransferMan 4r . . . . . . . . . . . . . . . . . . . . . 78

Table of contents

Tran sferMan

English (EN)

7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.1 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.2 Capillary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.3 Control board and display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.4 Joystick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.1.5 Software and parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.2.1 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.2.2 Error. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

8 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.1 Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.3 Service and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

8.4 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

9.2 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

9.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.1 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.3 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.4 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

11 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

11.1 TransferMan 4r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

11.2 Accessories for TransferMan 4r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

11.3 Tools for TransferMan 4r. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

11.4 Microscope adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

11.5 Accessories for adapters for microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

11.6 Capillaries for research applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

11.7 Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

11.8 Universal capillary holder and accessories. . . . . . . . . . . . . . . . . . . . . . . . . . 91

11.9 CellTram and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

11.10 FemtoJet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

11.11 FemtoJet express. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

11.12 Accessories for FemtoJet/FemtoJet express . . . . . . . . . . . . . . . . . . . . . . . . . 93

11.13 PiezoXpert. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

Operating instructions

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1 Operating instructions

1.1 Using this manual

 Read this operating manual completely before using the device for the first time.

Please also note the operating instructions for the accessories, if applicable.

 This operating manual is part of the product. Thus, it must always be easily accessible.  Enclose this operating manual when transferring the device to third parties.  If this manual is lost, please request another one. You will find the current version on

our webpage www.eppendorf.com/worldwide

1.2 Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1 Danger symbols

Hazard point Cuts

Material damage Electric shock

Biohazard

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WAR NIN G Can lead to severe injuries or death.

CAUTION Can lead to light to moderate injuries.

ATTENTION May lead to material damage.

1.3 Symbols used

Depiction Meaning

Actions in the specified order

 Actions without a specified order

• List:

Text Display or software texts

Additional information

2 Product description

2.1 Delivery package

Quantity Description

1X-module

1Y-module

1Z-module

1 YZ-connector

1 Swivel joint

1 Angle head

1 Control board

1Mains/power cord

1Cable sheathing

1 Operating manual

2.1.1 Tools

Quantity Description

7 Allen key

1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6 mm

1 3 mm Allen torque key

1 1.3 mm Allen key

1 Tool bag

Product description

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2.1.2 Accessories

Quantity Description

1 Connecting cable for FemtoJet/FemtoJet express

2 Positioning aids for capillary holders

1 Spare parts kit

1Label

2.2 Features

The micromanipulator TransferMan 4r has been especially developed for work processes that require intuitive movement of the capillary.

The TransferMan 4r combines the classical benefits of a mechanical system with the benefits of an accurate electrically driven system.

The capillary is controlled by a joystick. The joystick has an inner (proportional) and an

Product description

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outer (dynamic) movement range. In the inner range, the joystick movement is transferred directly to the capillary. In the outer range, a greater forwards or backwards movement of the joystick results in an acceleration of the capillary movement. The movement ranges enable moving to any position in the working range of the micromanipulator.

Proportional movement is suitable for all working techniques that require intuitive, sensitive handling, e.g., intracytoplasmic sperm injection (ICSI) and the transfer of stem cells into blastocysts.

The software control provides predefined applications, freely programmable softkey functions, a freely programmable application and the storage of different positions in all space coordinates.

2.3 Main illustration

The motor module is mounted on a special microscope adapter or on a free-standing stand (magnetic). The control board is mechanically separated from the motor module.

Abb. 2-1:TransferMan 4r — right side mounting

Fig. 2-1: TransferMan 4r — right side mounting

1 Motor module 2 Control board

Product description

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2.3.1 Motor module

The motor module is made up of three modules. The capillary can be moved in all three spatial axes due to the layout of the modules. The X-module with the capillary can be swung out of the work area using the swivel joint. The injection angle of the capillary can be set to any angle on the angle head.

Abb. 2-2:Motor module

Fig. 2-2: Motor module

1Z-module

2YZ-connector

3 Angle head

4Y-module

5Swivel joint

6X-module

Product description

home

fine

coarse

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2.3.2 Control board

The control board contains the key pad, the display and the joystick, and, to the side, the selection dial. The direction of movement and the speed of the joystick are transferred to the capillary. The responsitivity of the movement and the size of the working range are predefined in the software setting. On the control panel, the working range can be selected and individually changed using the selection dial.

Abb. 2-3:Control board - Front

Fig. 2-3: Control board - Front

1Joystick

Proportional and dynamic movement

2Display

3Control panel

4 Selection dial

For increasing or reducing the working range

Abb. 2-4:Control board - rear

X-Axis

Y-Axis

ext. Device

Z-Axis

SERVICE

Fig. 2-4: Control board - rear

Product description

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1Mains power switch On/Off

2 Connection for an external device

Foot control, FemtoJet, FemtoJet express or PiezoXpert

3 Connection for a Y-module

4 Connection for an X-module

5 Connection for a Z-module

6 Service interface

7 Mains/power connection

8Fuse

Product description

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English (EN)

2.4 Control panel

With the keys on the control panel you can switch on the control board and select the size of the working range. Via the softkeys you can open the applications, execute the functions, navigate the menu and set the parameters.

Abb. 2-5:Control panel

Fig. 2-5: Control panel

1 home key

Moves the capillary out of the working range to a defined position

2Display

Displays the software

3 standby key

Switches the control board on or off or cancels automatic movements

4Softkeys 1 – 5

For selecting the application, triggering the function, navigating or setting parameter values

fine

x–fine

homecoarse

5 menu key

Opens the menu

6 fine/x-fine key

Sets the medium or small working range

7 coarse key

Sets the large working range

Product description

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English (EN)

2.5 Joystick

The joystick moves the capillary in all three spatial axes. The joystick movement is transferred directly to the capillary in the proportional range. In the dynamic range, the movement of the capillary is accelerated the further the joystick is moved forwards and backwards.

Abb. 2-6:Joystick

Fig. 2-6: Joystick

1Joystick key

2Swivel

2Lower part

Controls movements in the X and Y-axis

Controls movements in the Z-axis

Abb. 2-7:Movement ranges of the joystick

Fig. 2-7: Movement ranges of the joystick

1 Proportional range 2 Dynamic range

Product description

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English (EN)

2.5.1 Proportional range

In the proportional range, the capillary will move as quickly or slowly as the joystick is moved. The travel of the capillary is also proportional to the distance that the joystick has been moved forwards and backwards. The movement of the capillary will stop as soon as the joystick is no longer moved or when the position is reached at which the joystick was stopped. There is a noticeable stop on the outer limit of the proportional range. This stop is in a narrow zone in which moving the joystick sideways does not result in any further movement of the capillary.

The size of the proportional range depends on the working range selected.

2.5.2 Dynamic range

After the stop, the dynamic range of the joystick begins: when the joystick is moved against the spring-loaded stop, the capillary starts moving in the direction of the joystick movement. The movement stops when the joystick is released and it drops back into the zone due to the spring force of the stop. The speed of the capillary in the dynamic range is increased dynamically by a stronger pressure against the stop.

The size of the dynamic range is limited by the movement range of the modules (X, Y and Z).

2.5.3 Direction of movement of the joystick

The joystick can be moved along the horizontal plane. This controls the motors of the X-module and the Y-module. The joystick can be moved in one axis at a time or in a combination of axes.

Abb. 2-8:Movement in the X and Y-axis

 Move the capillary in the horizontal

direction (X and Y-axis).

Fig. 2-8: Movement in the X and Y-axis

Product description

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English (EN)

2.5.4 Direction of movement of the rotating wheel

The rotating wheel on the joystick moves the motor module in the vertical axis. This activates the motor of the Z-module.

Abb. 2-9:Movement in the Z-axis

 Move the capillary in the vertical

direction (Z-axis).

Fig. 2-9: Movement in the Z-axis

2.5.5 Joystick key functions

Abb. 2-10:No movement of the capillary

 Uncouple the joystick from the motor

module.

 Trigger functions (e.g., switch between

saved positions).

Fig. 2-10: No movement of the capillary

2.6 Working range

There are three working ranges within the movement range of the joystick. For each working range, a radius with a resulting speed ratio is preset. The radius can be set using the selection dial on the control board and in the Speed menu.

Working ranges:

• coarse – for a large working range

• fine – for a medium working range

• x-fine – for a small working range

Product description

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Abb. 2-11:Large working r ange –

Fig. 2-11: Large working range – coarse

Abb. 2-12:Medium working ra nge –

Fig. 2-12: Medium working range – fine

Abb. 2-13:Small working ran ge –

 Move capillary over a large distance.  Position capillary roughly and quickly.

 Move capillary over a medium distance.  Position capillary precisely.

 Move capillary over a very short

distance.

 Position capillary very precisely and

slowly.

 Active for x-fine working range larger

than 0.

Fig. 2-13: Small working range – x-fine

Product description

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2.7 Resulting speed

In the inner (proportional) range, the speed of the capillary is dependent on the speed at which the joystick is moved forwards and backwards and the size of the set working range. If the joystick is moved forwards and backwards at the same speed with, e.g., the setting fine or coarse, the resulting speed with the smaller fine working range is less than with the larger coarse range.

The speed of the outer (dynamic) range is coupled to the selected working range. The coupling factor (Dyn-factor) can be changed and adjusted in the Installation menu. Since the speed results from the working range, the settings for Coarse fine and x-fine can be adjusted in the Speed menu.

Safety

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English (EN)

3Safety

3.1 Intended use

The TransferMan 4r has been designed and manufactured for use in biological, chemical and physical research. It is used for the precise positioning of microcapillaries and similar tools as well as for the transfer of very small sample volumes.

The TransferMan 4r has been designed and manufactured for research use only.

The TransferMan 4r is intended exclusively for indoor use and for operation by qualified staff.

3.2 Warnings for intended use

WARNING! Risk of injury due to flying capillaries and glass splinters.

If exposed to high pressures, capillaries may detach themselves from the grip heads and become projectiles. Capillaries can crack as a result of incorrect handling.

 Wear protective goggles.  Never aim capillaries at people.  Use capillaries with an outer diameter that matches the grip head

specifications.

 Always mount / dismount capillaries when they are depressurized.  Mount the capillary correctly in the grip head.  Do not touch the capillary with the Petri dish or other objects.

CAUTION! Risk of cuts from broken capillaries.

Capillaries are made of glass and are very fragile.

 Wear your personal protective equipment (PPE).  Always mount capillaries depressurized.  Never aim capillaries at people.  Handle the capillaries very carefully.

NOTICE! Mechanical damage to the motor modules.

Excessive load leads to increment errors or destruction of the drive.

 Do not drive the modules against mechanical obstructions.  Do not hold any objects near the modules.  Load the motor module with a maximum of 100 g.

WARNING! Damages to health due to infectious liquids and pathogenic germs.

 When handling infectious liquids and pathogenic germs, observe the national

regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes.

 Wear personal protective equipment.  Consult the "Laboratory Biosafety Manual" (Source: World Health

Organization, Laboratory Biosafety Manual, as amended) for comprehensive regulations on the handling of risk group II germs or biological materials.

3.3 Warning signs on the device

Warning symbol Meaning

Warns of the risk of injury caused by capillary tips

Warns of the danger of crushing on the motor module

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Safety

English (EN)

Read the operating manual

3.4 Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

• The device is not used in accordance with the operating manual.

• The device is used outside of its intended use.

• The device is used with accessories or consumables which are not recommended by Eppendorf.

• The device is maintained or repaired by people not authorized by Eppendorf.

• The user makes unauthorized changes to the device.

Installation

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4Installation

4.1 Preparing installation

NOTICE! Damage to the control board as a result of incorrect handling.

 Grasp the control board on the housing.  Do not lift the control board using the joystick.  Never place the control board on the joystick.

Keep the packaging and the transport securing devices for later transport or storage.

Do not operate the device if there is visible damage to the device itself and/or to its packaging.

1. Check the packaging for damage.

2. Carefully remove the motor module and the control board from the packaging.

3. Check that everything is included in the delivery.

4. Check the modules, the control board and the accessories for damage.

4.1.1 If there is any damage, make a claim

1. Contact customer service.

4.1.2 Delivery incomplete

1. Contact customer service.

4.1.3 Microscope adapter assembly

The microscope adapter is not included in the scope of delivery and must be ordered separately.

1. Assemble the microscope adapter in accordance with the assembly instructions for the microscope adapter.

4.2 Selecting the location

Select the location for the device according to the following criteria:

• Mains connection in accordance with the name plate.

• A bench with a horizontal and even work surface which is designed to support the weight of the device.

• A mat or table that is cushioned against vibrations.

• The location is protected from direct sunlight and drafts.

The mains/power switch and cutting unit of the mains/power line must be easily accessible during operation (e.g, residual current circuit breaker).

4.3 Mounting overview

5 6

8910

Abb. 4-1:Overview for right-hand side mounting

Fig. 4-1: Overview for right-hand side mounting

Installation

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1 Z-module holder

For left side mounting

2Microscope adapter

3 Z-module holder

For right side mounting

4Z-module

5Y-module

6Swivel joint

7X-module

8 Universal capillary holder

(not included in the delivery package)

9Angle head

10 YZ-connector

Installation

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English (EN)

4.3.1 Module (X, Y, Z)

Abb. 4-2:Y-module

Fig. 4-2: Y-module

1Cable

2Module identification

3Movable rail

4Scale

Movement range of the rail

5Fixed rail

4.3.2 Z-module holder

Abb. 4-3:Z-module holder, front and back

Fig. 4-3: Z-module holder, front and back

Installation

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1Slider

2Screw

Attach Z-module

4.3.3 Slider

Abb. 4-4:Slider

Fig. 4-4: Slider

1Slider

2Lock washer

3Screw

Attach Z-module holder to the adapter

4 Stop angle

3Flat washer

4Screw

Installation

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4.3.4 Angle head

Abb. 4-5:Angle head

Fig. 4-5: Angle head

1 Identification

For setting the angle

2Knurled screw

For setting the injection angle

3 Universal capillary holder

(not included in the delivery package)

4 Positioning aid

5Knurled screw

For fixing the universal capillary holder

4.3.5 Swivel joint

Abb. 4-6:Swivel joint for right side mounting

Installation

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6 7

Fig. 4-6: Swivel joint for right side mounting

1Slider

Y-module

2Turntable

3Allen screws

4Slider

X-module

5Stop plate

6Upper joint

7Mounting mark

| stands for left side mounting || stands for right side mounting

8Lower joint

4.4 Mounting the motor module

The motor module can be mounted on the right-hand or left-hand side of the microscope adapter. The following describes mounting on the right-hand side. For mounting on the left-hand side, the swivel joint and the angle head must be modified.

The motor module is mounted as standard on an inverse microscope. It can also be mounted on a universal stand. Mounting on a universal stand is described in the corresponding manual.

Installation

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4.4.1 Mounting the Z-module

Prerequisites

• 3 mm Allen torque key

1. Push the Z-module holder onto the microscope adapter and tighten.

2. Push the fixed rail onto the Z-module holder until it reaches the stop angle and tighten the screw. The connecting cable faces to the rear.

4.4.2 Mounting the Y-module

Prerequisites

• 3 mm Allen torque key

Installation

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1. Push the YZ-connector onto the fixed rail of the Y-module and tighten.

2. Push the Y-module with the YZ-connector onto the Z-module and tighten.

4.4.3 Mounting the X-module

Prerequisites

• 3 mm Allen torque key

1. Push the swivel joint onto the fixed rail of the X-module and tighten.

Installation

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4.4.4 Mounting the angle head

Prerequisites

• 3 mm Allen torque key

2. Push the swivel joint with the X-module onto the Y-module and tighten.

1. Push the angle head onto the X-module.

2. Slightly tighten the angle head.

+ 72 hidden pages