Read this operating manual completely before using the device for the first time.
Please also note the operating instructions for the accessories, if applicable.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. You will find the current version on
our webpage www.eppendorf.com/worldwide
1.2Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and
danger levels:
1.2.1 Danger symbols
Hazard pointCuts
Material damageElectric shock
Biohazard
.
1.2.2 Danger levels
DANGERWill lead to severe injuries or death.
WAR NIN GCan lead to severe injuries or death.
CAUTIONCan lead to light to moderate injuries.
ATTENTIONMay lead to material damage.
1.3Symbols used
Depiction Meaning
1.
2.
Actions in the specified order
Actions without a specified order
• List:
Text Display or software texts
Additional information
2Product description
2.1Delivery package
QuantityDescription
1X-module
1Y-module
1Z-module
1YZ-connector
1Swivel joint
1Angle head
1Control board
1Mains/power cord
1Cable sheathing
1Operating manual
2.1.1 Tools
QuantityDescription
7Allen key
1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6 mm
13 mm Allen torque key
11.3 mm Allen key
1Tool bag
Product description
Tran sferMan
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English (EN)
9
2.1.2 Accessories
QuantityDescription
1Connecting cable for FemtoJet/FemtoJet express
2Positioning aids for capillary holders
1Spare parts kit
1Label
2.2Features
The micromanipulator TransferMan 4r has been especially developed for work processes
that require intuitive movement of the capillary.
The TransferMan 4r combines the classical benefits of a mechanical system with the
benefits of an accurate electrically driven system.
The capillary is controlled by a joystick. The joystick has an inner (proportional) and an
Product description
1
2
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outer (dynamic) movement range. In the inner range, the joystick movement is
transferred directly to the capillary. In the outer range, a greater forwards or backwards
movement of the joystick results in an acceleration of the capillary movement. The
movement ranges enable moving to any position in the working range of the
micromanipulator.
Proportional movement is suitable for all working techniques that require intuitive,
sensitive handling, e.g., intracytoplasmic sperm injection (ICSI) and the transfer of stem
cells into blastocysts.
The software control provides predefined applications, freely programmable softkey
functions, a freely programmable application and the storage of different positions in all
space coordinates.
2.3Main illustration
The motor module is mounted on a special microscope adapter or on a free-standing
stand (magnetic). The control board is mechanically separated from the motor module.
Abb. 2-1:TransferMan 4r — right side mounting
Fig. 2-1:TransferMan 4r — right side mounting
1 Motor module2 Control board
Product description
1
2
3
4
5
6
Tran sferMan
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2.3.1 Motor module
The motor module is made up of three modules. The capillary can be moved in all three
spatial axes due to the layout of the modules. The X-module with the capillary can be
swung out of the work area using the swivel joint. The injection angle of the capillary can
be set to any angle on the angle head.
Abb. 2-2:Motor module
11
Fig. 2-2:Motor module
1Z-module
2YZ-connector
3 Angle head
4Y-module
5Swivel joint
6X-module
Product description
home
menu
fine
fine
coarse
4
1
2
3
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2.3.2 Control board
The control board contains the key pad, the display and the joystick, and, to the side, the
selection dial. The direction of movement and the speed of the joystick are transferred to
the capillary. The responsitivity of the movement and the size of the working range are
predefined in the software setting. On the control panel, the working range can be
selected and individually changed using the selection dial.
Abb. 2-3:Control board - Front
Fig. 2-3:Control board - Front
1Joystick
Proportional and dynamic movement
2Display
3Control panel
4 Selection dial
For increasing or reducing the working
range
Abb. 2-4:Control board - rear
X-Axis
Y-Axis
ext. Device
Z-Axis
SERVICE
45
6
7
3
2
1
8
Fig. 2-4:Control board - rear
Product description
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English (EN)
13
1Mains power switch On/Off
2 Connection for an external device
Foot control, FemtoJet, FemtoJet
express or PiezoXpert
3 Connection for a Y-module
4 Connection for an X-module
5 Connection for a Z-module
6 Service interface
7 Mains/power connection
8Fuse
Product description
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2.4Control panel
With the keys on the control panel you can switch on the control board and select the size
of the working range. Via the softkeys you can open the applications, execute the
functions, navigate the menu and set the parameters.
Abb. 2-5:Control panel
7
6
5
Fig. 2-5:Control panel
1 home key
Moves the capillary out of the working
range to a defined position
2Display
Displays the software
3 standby key
Switches the control board on or off or
cancels automatic movements
4Softkeys 1 – 5
For selecting the application, triggering
the function, navigating or setting
parameter values
fine
x–fine
menu
homecoarse
4
5 menu key
Opens the menu
6 fine/x-fine key
Sets the medium or small working range
7 coarse key
Sets the large working range
1
2
3
Product description
1
2
3
Tran sferMan
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English (EN)
2.5Joystick
The joystick moves the capillary in all three spatial axes. The joystick movement is
transferred directly to the capillary in the proportional range. In the dynamic range, the
movement of the capillary is accelerated the further the joystick is moved forwards and
backwards.
Abb. 2-6:Joystick
Fig. 2-6:Joystick
4r
15
1Joystick key
2Swivel
2Lower part
Controls movements in the X and Y-axis
Controls movements in the Z-axis
Abb. 2-7:Movement ranges of the joystick
1
2
Fig. 2-7:Movement ranges of the joystick
1 Proportional range2 Dynamic range
Product description
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2.5.1 Proportional range
In the proportional range, the capillary will move as quickly or slowly as the joystick is
moved. The travel of the capillary is also proportional to the distance that the joystick has
been moved forwards and backwards. The movement of the capillary will stop as soon as
the joystick is no longer moved or when the position is reached at which the joystick was
stopped. There is a noticeable stop on the outer limit of the proportional range. This stop
is in a narrow zone in which moving the joystick sideways does not result in any further
movement of the capillary.
The size of the proportional range depends on the working range selected.
2.5.2 Dynamic range
After the stop, the dynamic range of the joystick begins: when the joystick is moved
against the spring-loaded stop, the capillary starts moving in the direction of the joystick
movement. The movement stops when the joystick is released and it drops back into the
zone due to the spring force of the stop. The speed of the capillary in the dynamic range is
increased dynamically by a stronger pressure against the stop.
The size of the dynamic range is limited by the movement range of the modules (X, Y and
Z).
2.5.3 Direction of movement of the joystick
The joystick can be moved along the horizontal plane. This controls the motors of the
X-module and the Y-module. The joystick can be moved in one axis at a time or in a
combination of axes.
Abb. 2-8:Movement in the X and Y-axis
Move the capillary in the horizontal
direction (X and Y-axis).
Fig. 2-8:Movement in the X and Y-axis
Product description
Tran sferMan
English (EN)
2.5.4 Direction of movement of the rotating wheel
The rotating wheel on the joystick moves the motor module in the vertical axis. This
activates the motor of the Z-module.
Abb. 2-9:Movement in the Z-axis
Move the capillary in the vertical
direction (Z-axis).
Fig. 2-9:Movement in the Z-axis
2.5.5 Joystick key functions
Abb. 2-10:No movement of the capillary
Uncouple the joystick from the motor
module.
Trigger functions (e.g., switch between
saved positions).
®
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Fig. 2-10:No movement of the capillary
2.6Working range
There are three working ranges within the movement range of the joystick. For each
working range, a radius with a resulting speed ratio is preset. The radius can be set using
the selection dial on the control board and in the Speed menu.
Working ranges:
• coarse – for a large working range
• fine – for a medium working range
• x-fine – for a small working range
Product description
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Abb. 2-11:Large working r ange –
Fig. 2-11:Large working range – coarse
Abb. 2-12:Medium working ra nge –
Fig. 2-12:Medium working range – fine
Abb. 2-13:Small working ran ge –
Move capillary over a large distance.
Position capillary roughly and quickly.
Move capillary over a medium distance.
Position capillary precisely.
Move capillary over a very short
distance.
Position capillary very precisely and
slowly.
Active for x-fine working range larger
than 0.
Fig. 2-13:Small working range – x-fine
Product description
Tran sferMan
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English (EN)
2.7Resulting speed
In the inner (proportional) range, the speed of the capillary is dependent on the speed at
which the joystick is moved forwards and backwards and the size of the set working
range. If the joystick is moved forwards and backwards at the same speed with, e.g., the
setting fine or coarse, the resulting speed with the smaller fine working range is less than
with the larger coarse range.
The speed of the outer (dynamic) range is coupled to the selected working range. The
coupling factor (Dyn-factor) can be changed and adjusted in the Installation menu. Since
the speed results from the working range, the settings for Coarse fine and x-fine can be
adjusted in the Speed menu.
19
Safety
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English (EN)
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3Safety
3.1Intended use
The TransferMan 4r has been designed and manufactured for use in biological, chemical
and physical research. It is used for the precise positioning of microcapillaries and similar
tools as well as for the transfer of very small sample volumes.
The TransferMan 4r has been designed and manufactured for research use only.
The TransferMan 4r is intended exclusively for indoor use and for operation by qualified
staff.
3.2Warnings for intended use
WARNING! Risk of injury due to flying capillaries and glass splinters.
If exposed to high pressures, capillaries may detach themselves from the grip
heads and become projectiles.
Capillaries can crack as a result of incorrect handling.
Wear protective goggles.
Never aim capillaries at people.
Use capillaries with an outer diameter that matches the grip head
specifications.
Always mount / dismount capillaries when they are depressurized.
Mount the capillary correctly in the grip head.
Do not touch the capillary with the Petri dish or other objects.
CAUTION! Risk of cuts from broken capillaries.
Capillaries are made of glass and are very fragile.
Wear your personal protective equipment (PPE).
Always mount capillaries depressurized.
Never aim capillaries at people.
Handle the capillaries very carefully.
NOTICE! Mechanical damage to the motor modules.
Excessive load leads to increment errors or destruction of the drive.
Do not drive the modules against mechanical obstructions.
Do not hold any objects near the modules.
Load the motor module with a maximum of 100 g.
WARNING! Damages to health due to infectious liquids and pathogenic
germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.
Wear personal protective equipment.
Consult the "Laboratory Biosafety Manual" (Source: World Health
Organization, Laboratory Biosafety Manual, as amended) for comprehensive
regulations on the handling of risk group II germs or biological materials.
3.3Warning signs on the device
Warning symbolMeaning
Warns of the risk of injury caused by capillary tips
Warns of the danger of crushing on the motor module
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Read the operating manual
3.4Information on product liability
In the following cases, the designated protection of the device may be compromised.
Liability for any resulting property damage or personal injury is then transferred to the
operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
Installation
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English (EN)
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4Installation
4.1Preparing installation
NOTICE! Damage to the control board as a result of incorrect handling.
Grasp the control board on the housing.
Do not lift the control board using the joystick.
Never place the control board on the joystick.
Keep the packaging and the transport securing devices for later transport or
storage.
Do not operate the device if there is visible damage to the device itself and/or to
its packaging.
1. Check the packaging for damage.
2. Carefully remove the motor module and the control board from the packaging.
3. Check that everything is included in the delivery.
4. Check the modules, the control board and the accessories for damage.
4.1.1 If there is any damage, make a claim
1. Contact customer service.
4.1.2 Delivery incomplete
1. Contact customer service.
4.1.3 Microscope adapter assembly
The microscope adapter is not included in the scope of delivery and must be ordered
separately.
1. Assemble the microscope adapter in accordance with the assembly instructions for the
microscope adapter.
4.2Selecting the location
Select the location for the device according to the following criteria:
• Mains connection in accordance with the name plate.
• A bench with a horizontal and even work surface which is designed to support the
weight of the device.
• A mat or table that is cushioned against vibrations.
• The location is protected from direct sunlight and drafts.
The mains/power switch and cutting unit of the mains/power line must be easily
accessible during operation (e.g, residual current circuit breaker).
4.3Mounting overview
1
2
3
56
7
4
8910
Abb. 4-1:Overview for right-hand side mounting
Fig. 4-1:Overview for right-hand side mounting
Installation
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4r
1 Z-module holder
For left side mounting
2Microscope adapter
3 Z-module holder
For right side mounting
4Z-module
5Y-module
6Swivel joint
7X-module
8 Universal capillary holder
(not included in the delivery package)
9Angle head
10 YZ-connector
Installation
Y
1
2
3
4
5
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4.3.1 Module (X, Y, Z)
Abb. 4-2:Y-module
Fig. 4-2:Y-module
1Cable
2Module identification
3Movable rail
4Scale
Movement range of the rail
5Fixed rail
4.3.2 Z-module holder
2
3
1
4
1
2
3
4
Abb. 4-3:Z-module holder, front and back
Fig. 4-3:Z-module holder, front and back
Installation
Tran sferMan
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1Slider
2Screw
Attach Z-module
4.3.3 Slider
Abb. 4-4:Slider
Fig. 4-4:Slider
1Slider
2Lock washer
3Screw
Attach Z-module holder to the adapter
4 Stop angle
3Flat washer
4Screw
Installation
Tran sferMan
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English (EN)
4.3.4 Angle head
Abb. 4-5:Angle head
®
4r
Fig. 4-5:Angle head
1 Identification
For setting the angle
2Knurled screw
For setting the injection angle
3 Universal capillary holder
(not included in the delivery package)
1
2
5
4 Positioning aid
5Knurled screw
For fixing the universal capillary holder
3
4
4.3.5 Swivel joint
Abb. 4-6:Swivel joint for right side mounting
Installation
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4r
English (EN)
5
1
6
7
2
3
8
4
Fig. 4-6:Swivel joint for right side mounting
1Slider
Y-module
2Turntable
3Allen screws
4Slider
X-module
5Stop plate
6Upper joint
7Mounting mark
| stands for left side mounting
|| stands for right side mounting
8Lower joint
4.4Mounting the motor module
The motor module can be mounted on the right-hand or left-hand side of the microscope
adapter. The following describes mounting on the right-hand side. For mounting on the
left-hand side, the swivel joint and the angle head must be modified.
The motor module is mounted as standard on an inverse microscope. It can also
be mounted on a universal stand. Mounting on a universal stand is described in
the corresponding manual.
Installation
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4.4.1 Mounting the Z-module
Prerequisites
• 3 mm Allen torque key
1. Push the Z-module holder onto the
microscope adapter and tighten.
2. Push the fixed rail onto the Z-module
holder until it reaches the stop angle and
tighten the screw.
The connecting cable faces to the rear.
4.4.2 Mounting the Y-module
Prerequisites
• 3 mm Allen torque key
Installation
Tran sferMan
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1. Push the YZ-connector onto the fixed rail
of the Y-module and tighten.
2. Push the Y-module with the
YZ-connector onto the Z-module and
tighten.
29
4.4.3 Mounting the X-module
Prerequisites
• 3 mm Allen torque key
1. Push the swivel joint onto the fixed rail
of the X-module and tighten.
Installation
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4.4.4 Mounting the angle head
Prerequisites
• 3 mm Allen torque key
2. Push the swivel joint with the X-module
onto the Y-module and tighten.
1. Push the angle head onto the X-module.
2. Slightly tighten the angle head.
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