Eppendorf TransferMan4 m User Manual
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TransferMan® 4m
Operating manual
Copyright© 2013 Eppendorf AG, Hamburg.
Eppendorf
®
and the Eppendorf logo are registered trademarks of Eppendorf AG,
Hamburg, Germany.
Tran sferMan
®
4m, FemtoJet® and Eppendorf PiezoXpert® are registered trademarks of
Eppendorf AG, Hamburg, Germany.
Registered trademarks are not marked in all cases with ™ or ® in this manual.
No part of this publication may be reproduced without the prior permission of the
copyright owner.
5191 900.016-00/072013
Table of contents
TransferMan
®
4m
English (EN)
Operating manualTransferMan® 4 mseeon p.Fig.Tab.p.
English (EN)Operating manual
Table of contents
1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.3 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.3.1 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.3.2 Control board. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.4 Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.5 Joystick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.5.1 Proportional range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.5.2 Dynamic range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.5.3 Direction of movement of the joystick. . . . . . . . . . . . . . . . . . . . . . 16
2.5.4 Direction of movement of the rotating wheel . . . . . . . . . . . . . . . . 17
2.5.5 Joystick key functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.6 Working range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.7 Resulting speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.3 Warning signs on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.1.1 If there is any damage, make a claim . . . . . . . . . . . . . . . . . . . . . . 22
4.1.2 Delivery incomplete . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.1.3 Microscope adapter assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.3 Mounting overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.3.1 Module (X, Y, Z). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
4.3.2 Z-module holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
4.3.3 Slider . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
4.3.4 Angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
4.3.5 Swivel joint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
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4.4 Mounting the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
4.4.1 Mounting the Z-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
4.4.2 Mounting the Y-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
4.4.3 Mounting the X-module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4.4.4 Mounting the angle head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
4.5 Inserting the capillary holder into the angle head . . . . . . . . . . . . . . . . . . . . 30
4.6 Inserting the capillary into the capillary holder . . . . . . . . . . . . . . . . . . . . . . 31
4.6.1 Inserting Femtotips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
4.7 Setting the injection angle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
4.8 Aligning the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.8.1 Aligning the height . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.8.2 Aligning the depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.8.3 Aligning the width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
4.8.4 Aligning the angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
4.9 Entering mounting parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
4.9.1 Microscope and adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
4.9.2 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
4.9.3 Angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
4.10 Converting the swivel joint for left side mounting . . . . . . . . . . . . . . . . . . . . 36
4.11 Converting the angle head for left side mounting . . . . . . . . . . . . . . . . . . . . 39
4.12 Connect the motor module to the control board. . . . . . . . . . . . . . . . . . . . . . 40
4.13 Setting installation parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
4.13.1 First set-up wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
5 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
5.1 Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
5.1.1 Application display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
5.1.2 Display of coordinates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
5.1.3 Menu display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.2 Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.2.1 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
5.2.2 Application – Cell transfer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
5.2.3 Application – ICSI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.2.4 Application – DNA injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.2.5 Application – Basic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.2.6 Application – My application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.3 Main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
5.4 Navigating the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
5.4.1 Entering or changing parameters . . . . . . . . . . . . . . . . . . . . . . . . . 52
6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.1 Switching the device on or off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.1.1 Switching on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.1.2 Switching the device off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
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6.2 Activating or deactivating the control board. . . . . . . . . . . . . . . . . . . . . . . . . 53
6.2.1 Activating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.2.2 Deactivating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.3 Defining the start screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.3.1 Defining the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.3.2 Defining the selected application . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.4 Replacing the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.4.1 Manually positioning the capillary . . . . . . . . . . . . . . . . . . . . . . . . 55
6.4.2 Automatically positioning the capillary . . . . . . . . . . . . . . . . . . . . . 55
6.5 Changing the sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
6.6 Changing the size of the working range . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
6.6.1 Change parameters using the selection dial . . . . . . . . . . . . . . . . . 56
6.6.2 Changing parameters in the menu . . . . . . . . . . . . . . . . . . . . . . . . 57
6.7 Shifting the movement range of the capillary. . . . . . . . . . . . . . . . . . . . . . . . 57
6.7.1 Extending the movement range into the dynamic range . . . . . . . 57
6.7.2 Uncoupling and resetting the joystick. . . . . . . . . . . . . . . . . . . . . . 57
6.8 Capillary positions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.8.1 Saving a position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.8.2 Move to the position using the softkey . . . . . . . . . . . . . . . . . . . . . 58
6.8.3 Move to the position using the joystick button . . . . . . . . . . . . . . . 59
6.8.4 Deleting a stored position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.8.5 Overwriting a stored position . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.9 Vertical limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.9.1 Defining the lower limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
6.9.2 Deleting the lower limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
6.9.3 Defining the upper limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
6.9.4 Deleting the upper limit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6.10 Horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6.10.1 Defining the horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6.10.2 Deleting the horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6.11 Speed function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.11.1 Speed menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.11.2 Setting the parameter for Speed . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.12 Step injection function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
6.12.1 Step injection menu and parameters. . . . . . . . . . . . . . . . . . . . . . . 63
6.12.2 Executing the Step injection function . . . . . . . . . . . . . . . . . . . . . . 64
6.13 Home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
6.13.1 Home menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
6.13.2 Setting the parameter for Home . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.13.3 Move the capillary out with the home key . . . . . . . . . . . . . . . . . . 65
6.13.4 Move the capillary back with the home key . . . . . . . . . . . . . . . . . 65
6.13.5 Setting the offset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
6.13.6 Terminate the home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
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6.14 Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
6.14.1 Clean menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
6.14.2 Setting the parameter for Clean . . . . . . . . . . . . . . . . . . . . . . . . . . 67
6.14.3 Execute the Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
6.14.4 Terminate the Clean function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
6.15 PiezoXpert function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
6.15.1 PiezoXpert menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . 68
6.15.2 Execute the PiezoXpert function . . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.16 Installation function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.16.1 Installation menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.16.2 Installation parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
6.16.3 Control board parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
6.16.4 Joystick parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
6.16.5 Module parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
6.17 Function function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
6.17.1 Function menu and parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . 71
6.17.2 Executing Zero coordin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
6.17.3 Executing Center motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
6.17.4 Executing User default . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
6.18 Softkeys function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
6.18.1 Softkeys menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
6.18.2 Execute Softkeys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
6.18.3 Execute Joystick key . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
6.19 Change appl. function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
6.19.1 Change appl. menu and parameters . . . . . . . . . . . . . . . . . . . . . . . 75
6.19.2 Defining the selected application as the start screen . . . . . . . . . . 75
6.19.3 Defining the application as the start screen . . . . . . . . . . . . . . . . . 75
6.20 Service function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
6.20.1 Service menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
6.20.2 Execute the Selftest function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
6.21 Resetting parameters to the factory settings . . . . . . . . . . . . . . . . . . . . . . . . 77
6.21.1 Perform reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
6.21.2 Carrying out a reset in the menu . . . . . . . . . . . . . . . . . . . . . . . . . . 77
7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
7.2.1 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
7.2.2 Error. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
8 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
8.1 Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
8.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
8.3 Service and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
8.4 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Table of contents
TransferMan
®
4m
English (EN)
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
9.2 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
9.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
10.1 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
10.3 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
10.4 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
11 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
11.1 TransferMan 4m . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
11.2 Accessories for TransferMan 4m . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
11.3 Tools for TransferMan 4m. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
11.4 Microscope adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
11.5 Accessories for adapters for microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
11.6 Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
11.7 CellTram and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
7
Operating instructions
Tran sferMan
8
English (EN)
®
4m
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time.
Please also note the operating instructions for the accessories, if applicable.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. You will find the current version on
our webpage www.eppendorf.com/worldwide
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and
danger levels:
1.2.1 Danger symbols
Hazard point Cuts
Material damage Electric shock
Biohazard
.
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WAR NIN G Can lead to severe injuries or death.
CAUTION Can lead to light to moderate injuries.
ATTENTION May lead to material damage./Paragraph
1.3 Symbols used
Depiction Meaning
1.
2.
Actions in the specified order
Actions without a specified order
• List:
Text Display or software texts
Additional information
Product description
TransferMan
2 Product description
2.1 Delivery package
Quantity Description
1X-module
1Y-module
1Z-module
1 YZ-connector
1 Swivel joint
1 Angle head
1 Control board
1Mains/power cord
1Cable sheathing
1 Operating manual
2.1.1 Tools
Quantity Description
7 1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6 mm Allen key
1 3 mm Allen torque key
1 1.3 mm Allen key
1 Tool bag
®
4m
English (EN)
9
2.1.2 Accessories
Quantity Description
1 Connecting cable for FemtoJet/FemtoJet express
2 Positioning aids for capillary holders
1 Spare parts kit
1Label
2.2 Features
The micromanipulator TransferMan 4m has been especially developed for work processes
that require intuitive movement of the capillary.
The TransferMan 4m combines the classical benefits of a mechanical system with the
benefits of an accurate electrically driven system.
The capillary is controlled by a joystick. The joystick has an inner (proportional) and an
outer (dynamic) movement range. In the inner range, the joystick movement is
Product description
1
2
Tran sferMan
10
®
4m
English (EN)
transferred directly to the capillary. In the outer range, a greater forwards or backwards
movement of the joystick results in an acceleration of the capillary movement. The
movement ranges enable moving to any position in the working range of the
micromanipulator.
Proportional movement is suitable for all working techniques that require intuitive,
sensitive handling, e.g., intracytoplasmic sperm injection (ICSI) and the transfer of stem
cells into blastocysts.
The software control provides predefined applications, freely programmable softkey
functions, a freely programmable application and the storage of different positions in all
space coordinates.
2.3 Main illustration
The motor module is mounted on a special microscope adapter or on a free-standing
stand (magnetic). The control board is mechanically separated from the motor module.
Abb. 2-1:TransferMan 4m — right side mounting
Fig. 2-1: TransferMan 4m — right side mounting
1 Motor module 2 Control board
Product description
1
2
3
4
5
6
TransferMan
®
4m
English (EN)
2.3.1 Motor module
The motor module is made up of three modules. The capillary can be moved in all three
spatial axes due to the layout of the modules. The X-module with the capillary can be
swung out of the work area using the swivel joint. The injection angle of the capillary can
be set to any angle on the angle head.
Abb. 2-2:Motor module
11
Fig. 2-2: Motor module
1Z-module
2YZ-connector
3 Angle head
4Y-module
5Swivel joint
6X-module
Product description
home
menu
fine
fine
coarse
4
1
2
3
Tran sferMan
12
®
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English (EN)
2.3.2 Control board
The control board contains the key pad, the display and the joystick, and, to the side, the
selection dial. The direction of movement and the speed of the joystick are transferred to
the capillary. The responsitivity of the movement and the size of the working range are
predefined in the software setting. On the control panel, the working range can be
selected and individually changed using the selection dial.
Abb. 2-3:Control board - Front
Fig. 2-3: Control board - Front
1Joystick
Proportional and dynamic movement
2Display
3Control panel
4 Selection dial
For increasing or reducing the working
range
Abb. 2-4:Control board - rear
X-Axis
Y-Axis
ext. Device
Z-Axis
SERVICE
45
6
7
3
2
1
8
Fig. 2-4: Control board - rear
Product description
TransferMan
®
4m
English (EN)
13
1Mains power switch On/Off
2 Connection for an external device
Foot control, FemtoJet, FemtoJet
express or PiezoXpert
3 Connection for a Y-module
4 Connection for an X-module
5 Connection for a Z-module
6 Service interface
7 Mains/power connection
8Fuse
Product description
Tran sferMan
14
®
4m
English (EN)
2.4 Control panel
With the keys on the control panel you can switch on the control board and select the size
of the working range. Via the softkeys you can open the applications, execute the
functions, navigate the menu and set the parameters.
Abb. 2-5:Control panel
7
6
5
Fig. 2-5: Control panel
1 home key
Moves the capillary out of the working
range to a defined position
2Display
Displays the software
3 standby key
Switches the control board on or off or
cancels automatic movements
4Softkeys 1 – 5
For selecting the application, triggering
the function, navigating or setting
parameter values
fine
x–fine
menu
homecoarse
4
5 menu key
Opens the menu
6 fine/x-fine key
Sets the medium or small working range
7 coarse key
Sets the large working range
1
2
3
Product description
1
2
3
TransferMan
®
4m
English (EN)
2.5 Joystick
The joystick moves the capillary in all three spatial axes. The joystick movement is
transferred directly to the capillary in the proportional range. In the dynamic range, the
movement of the capillary is accelerated the further the joystick is moved forwards and
backwards.
Abb. 2-6:Joystick
Fig. 2-6: Joystick
15
1Joystick key
2Swivel
2Lower part
Controls movements in the X and Y-axis
Controls movements in the Z-axis
Abb. 2-7:Movement ranges of the joystick
1
2
Fig. 2-7: Movement ranges of the joystick
1 Proportional range 2 Dynamic range
Product description
Tran sferMan
16
®
4m
English (EN)
2.5.1 Proportional range
In the proportional range, the capillary will move as quickly or slowly as the joystick is
moved. The travel of the capillary is also proportional to the distance that the joystick has
been moved forwards and backwards. The movement of the capillary will stop as soon as
the joystick is no longer moved or when the position is reached at which the joystick was
stopped. There is a noticeable stop on the outer limit of the proportional range. This stop
is in a narrow zone in which moving the joystick sideways does not result in any further
movement of the capillary.
The size of the proportional range depends on the working range selected.
2.5.2 Dynamic range
After the stop, the dynamic range of the joystick begins: when the joystick is moved
against the spring-loaded stop, the capillary starts moving in the direction of the joystick
movement. The movement stops when the joystick is released and it drops back into the
zone due to the spring force of the stop. The speed of the capillary in the dynamic range is
increased dynamically by a stronger pressure against the stop.
The size of the dynamic range is limited by the movement range of the modules (X, Y and
Z).
2.5.3 Direction of movement of the joystick
The joystick can be moved along the horizontal plane. This controls the motors of the
X-module and the Y-module. The joystick can be moved in one axis at a time or in a
combination of axes.
Abb. 2-8:Movement in the X and Y-axis
Move the capillary in the horizontal
direction (X and Y-axis).
Fig. 2-8: Movement in the X and Y-axis
Product description
TransferMan
English (EN)
2.5.4 Direction of movement of the rotating wheel
The rotating wheel on the joystick moves the motor module in the vertical axis. This
activates the motor of the Z-module.
Abb. 2-9:Movement in the Z-axis
Move the capillary in the vertical
direction (Z-axis).
Fig. 2-9: Movement in the Z-axis
2.5.5 Joystick key functions
Abb. 2-10:No movement of the capillary
Uncouple the joystick from the motor
module.
Trigger functions (e.g., switch between
saved positions).
®
4m
17
Fig. 2-10: No movement of the capillary
2.6 Working range
There are three working ranges within the movement range of the joystick. For each
working range, a radius with a resulting speed ratio is preset. The radius can be set using
the selection dial on the control board and in the Speed menu.
Working ranges:
• coarse – for a large working range
• fine – for a medium working range
• x-fine – for a small working range
Product description
Tran sferMan
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English (EN)
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Abb. 2-11:Large working r ange – coarse
Fig. 2-11: Large working range – coarse
Abb. 2-12:Medium working ra nge – fine
Fig. 2-12: Medium working range – fine
Abb. 2-13:Small working ran ge – x-fine
Move capillary over a large distance.
Position capillary roughly and quickly.
Move capillary over a medium distance.
Position capillary precisely.
Move capillary over a very short
distance.
Position capillary very precisely and
slowly.
Active for x-fine working range larger
than 0.
Fig. 2-13: Small working range – x-fine
Product description
TransferMan
®
4m
English (EN)
2.7 Resulting speed
In the inner (proportional) range, the speed of the capillary is dependent on the speed at
which the joystick is moved forwards and backwards and the size of the set working
range. If the joystick is moved forwards and backwards at the same speed with, e.g., the
setting fine or coarse, the resulting speed with the smaller fine working range is less than
with the larger coarse range.
The speed of the outer (dynamic) range is coupled to the selected working range. The
coupling factor (Dyn-factor) can be changed and adjusted in the Installation menu. Since
the speed results from the working range, the settings for Coarse fine and x-fine can be
adjusted in the Speed menu.
19
Safety
Tran sferMan
20
English (EN)
®
4m
3Safety
3.1 Intended use
The TransferMan 4m has been designed and manufactured for use in the field of
intracytoplasmic sperm injection (ICSI) in human reproductive medicine. Therefore, the
TransferMan 4m is a medical device in accordance with Directive 93/42/EEC of the
European Union. The TransferMan 4m is intended exclusively for indoor use and for
operation by qualified staff.
3.2 Warnings for intended use
WARNING! Risk of injury due to flying capillaries and glass splinters.
If exposed to high pressures, capillaries may detach themselves from the grip
heads and become projectiles.
Capillaries can crack as a result of incorrect handling.
Wear protective goggles.
Never aim capillaries at people.
Use capillaries with an outer diameter that matches the grip head
specifications.
Always mount / dismount capillaries when they are depressurized.
Mount the capillary correctly in the grip head.
Do not touch the capillary with the Petri dish or other objects.
CAUTION! Risk of cuts from broken capillaries.
Capillaries are made of glass and are very fragile.
Wear your personal protective equipment (PPE).
Always mount capillaries depressurized.
Never aim capillaries at people.
Handle the capillaries very carefully.
NOTICE! Mechanical damage to the motor modules.
Excessive load leads to increment errors or destruction of the drive.
Do not drive the modules against mechanical obstructions.
Do not hold any objects near the modules.
Load the motor module with a maximum of 100 g.
WARNING! Damages to health due to infectious liquids and pathogenic
germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.
Wear personal protective equipment.
Consult the "Laboratory Biosafety Manual" (Source: World Health
Organization, Laboratory Biosafety Manual, as amended) for comprehensive
regulations on the handling of risk group II germs or biological materials.
3.3 Warning signs on the device
Warning symbol Meaning
Warns of the risk of injury caused by capillary tips
Warns of the danger of crushing on the motor module
TransferMan
English (EN)
Safety
®
4m
21
Read the operating manual
3.4 Information on product liability
In the following cases, the designated protection of the device may be compromised.
Liability for any resulting property damage or personal injury is then transferred to the
operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
Installation
Tran sferMan
22
English (EN)
®
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4 Installation
4.1 Preparing installation
NOTICE! Damage to the control board as a result of incorrect handling.
Grasp the control board on the housing.
Do not lift the control board using the joystick.
Never place the control board on the joystick.
Keep the packaging and the transport securing devices for later transport or
storage.
Do not operate the device if there is visible damage to the device itself and/or to
its packaging.
1. Check the packaging for damage.
2. Carefully remove the motor module and the control board from the packaging.
3. Check that everything is included in the delivery.
4. Check the modules, the control board and the accessories for damage.
4.1.1 If there is any damage, make a claim
1. Contact customer service.
4.1.2 Delivery incomplete
1. Contact customer service.
4.1.3 Microscope adapter assembly
The microscope adapter is not included in the scope of delivery and must be ordered
separately.
1. Assemble the microscope adapter in accordance with the assembly instructions for the
microscope adapter.
4.2 Selecting the location
Select the location for the device according to the following criteria:
• Mains connection in accordance with the name plate.
• A bench with a horizontal and even work surface which is designed to support the
weight of the device.
• A mat or table that is cushioned against vibrations.
• The location is protected from direct sunlight and drafts.
The mains/power switch and cutting unit of the mains/power line must be easily
accessible during operation (e.g, residual current circuit breaker).
4.3 Mounting overview
1
2
3
5 6
7
4
8910
Abb. 4-1:Overview for right-hand side mounting
Fig. 4-1: Overview for right-hand side mounting
Installation
TransferMan
English (EN)
®
4m
23
1 Z-module holder
For left side mounting
2Microscope adapter
3 Z-module holder
For right side mounting
4Z-module
5Y-module
6Swivel joint
7X-module
8 Universal capillary holder
(not included in the delivery package)
9Angle head
10 YZ-connector
Installation
Y
1
2
3
4
5
Tran sferMan
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®
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English (EN)
4.3.1 Module (X, Y, Z)
Abb. 4-2:Y-module
Fig. 4-2: Y-module
1Cable
2Module identification
3Movable rail
4Scale
Movement range of the rail
5Fixed rail
4.3.2 Z-module holder
2
3
1
4
1
2
3
4
Abb. 4-3:Z-module holder, front and back
Fig. 4-3: Z-module holder, front and back
Installation
TransferMan
English (EN)
®
4m
25
1Slider
2Screw
Attach Z-module
4.3.3 Slider
Abb. 4-4:Slider
Fig. 4-4: Slider
1Slider
2Lock washer
3Screw
Attach Z-module holder to the adapter
4 Stop angle
3Flat washer
4Screw
Installation
Tran sferMan
26
English (EN)
4.3.4 Angle head
Abb. 4-5:Angle head
®
4m
Fig. 4-5: Angle head
1 Identification
For setting the angle
2Knurled screw
For setting the injection angle
3 Universal capillary holder
(not included in the delivery package)
1
2
5
4 Positioning aid
5Knurled screw
For fixing the universal capillary holder
3
4
4.3.5 Swivel joint
Abb. 4-6:Swivel joint for right side mounting
Installation
TransferMan
®
4m
27
English (EN)
5
1
6
7
2
3
8
4
Fig. 4-6: Swivel joint for right side mounting
1Slider
Y-module
2Turntable
3Allen screws
4Slider
X-module
5Stop plate
6Upper joint
7Mounting mark
| stands for left side mounting
|| stands for right side mounting
8Lower joint
4.4 Mounting the motor module
The motor module can be mounted on the right-hand or left-hand side of the microscope
adapter. The following describes mounting on the right-hand side. For mounting on the
left-hand side, the swivel joint and the angle head must be modified.
The motor module is mounted as standard on an inverse microscope. It can also
be mounted on a universal stand. Mounting on a universal stand is described in
the corresponding manual.
Installation
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®
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4.4.1 Mounting the Z-module
Prerequisites
• 3 mm Allen torque key
1. Push the Z-module holder onto the
microscope adapter and tighten.
2. Push the fixed rail onto the Z-module
holder until it reaches the stop angle and
tighten the screw.
The connecting cable faces to the rear.
4.4.2 Mounting the Y-module
Prerequisites
• 3 mm Allen torque key
Installation
TransferMan
®
4m
English (EN)
1. Push the YZ-connector onto the fixed rail
of the Y-module and tighten.
2. Push the Y-module with the
YZ-connector onto the Z-module and
tighten.
29
4.4.3 Mounting the X-module
Prerequisites
• 3 mm Allen torque key
1. Push the swivel joint onto the fixed rail
of the X-module and tighten.
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